Relevant bibliographies by topics / Cytological techniques

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Cytological techniques'

Author: Grafiati
Published: 4 June 2021
Last updated: 28 July 2024

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Journal articles on the topic "Cytological techniques"

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Fives, Cassie, André Toulouse, Louise Kenny, Therese Brosnan, Julie McCarthy, and Brendan Fitzgerald. "Cytology Techniques Can Provide Insight into Human Placental Structure Including Syncytiotrophoblast Nuclear Spatial Organisation." Journal of Developmental Biology 11, no. 4 (December 15, 2023): 46. http://dx.doi.org/10.3390/jdb11040046.

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The aim of this study was to provide the first systematic description of human placental cytology appearances and to investigate syncytiotrophoblast nuclear organisation patterns using cytology techniques. Term placentas from normal pregnancies were sampled using fine-needle aspiration (FNA) and direct scrapes. Standard histological examination was also performed to exclude pathological changes in the placentas being studied. Both Papanicolaou-stained cytospin preparations and air-dried Giemsa slides from FNA provided high-quality material for cytological assessment with good cellularity. Among the key features of the cytology preparations were villous “microbiopsies” that allowed for the three-dimensional appreciation of villous branching patterns. Cytological appearances, including nuclear characteristics of villous cytotrophoblast and syncytiotrophoblast, were also well demonstrated. In microbiopsies and detached villous trophoblast sheets, complex patterns of syncytiotrophoblast nuclear organisation, not previously described cytologically, were observed, including irregular spacing of nuclei, syncytioplasm windows and linear nuclear arrangements. This study showed that placental cytology (a) provides technically excellent material for cytological evaluation, (b) confirms the presence of complex nuclear organisational patterns in the syncytiotrophoblast by eliminating the possibility of tangential sectioning artefact, (c) provides superior nuclear detail over standard histological sections and (d) may be an untapped research resource for the investigation of normal and pathological processes because of its ability to look at the placenta in a novel way and through its potential for both ex vivo and in vivo placental sampling.
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Singh, Shashikant, Prajwala Gupta, Purnima Malhotra, Minakshi Bhardwaj, and Desh Deepak. "Typical Carcinoid: Cytological Diagnosis of a Case and Mimickers in Cytological Sampling Techniques with Brief Review of Literature." Annals of Pathology and Laboratory Medicine 6, no. 3 (March 16, 2019): C32–36. http://dx.doi.org/10.21276/apalm.2329.

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Pakharukova, M. I., E. E. Lysak, K. V. Ryzhankova, M. A. Kotugina, L. N. Rebrikova, P. E. Ermolaeva, and Ya B. Beikin. "Application of cyto-histological correlation as a quality control in diagnosing cervical pathology." CLINICAL AND EXPERIMENTAL MORPHOLOGY 13, no. 1 (2024): 76–83. http://dx.doi.org/10.31088/cem2024.13.1.76-83.

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Introduction. In Russia, cervical cancer screening with cytological techniques faces a problem of quality control. Currently, it is necessary to standardize the methodology for comparing the results of morphological methods. The aim of the paper was to compare the findings of cytological and histological studies when diagnosing cervical pathology, classify discrepancies, and evaluate the effectiveness of the cytological method. Materials and methods. A comparative retrospective analysis of cytological examination followed by histological examination was carried out in 2,739 patients. Results. Both methods showed coincidence in 57.9% of cases. Minor and major discrepancies were determined in 29% and 13.1% of cases, respectively. The cytologic technique showed the following figures: sensitivity of 79.3%, specificity of 77.6%, positive prognostic value of 80%, and negative predictive value of 76.9%. When using the liquid-based cytology method, the number of true positive results was significantly higher, while that of false negative results was lower. The interval of up to 2 months between cytological and histological examinations yielded the highest rate of true positive and the lowest rate of false negative results. Conclusion. Comparing the results of morphological methods is most likely to be the best option for ensuring quality control of laboratory tests. This should be a guideline with a subsequent revision of samples in the presence of discrepancies, analysis of their causes, and evaluation of method effectiveness.
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Barnard, N. "Routine Cytological Staining Techniques: Theoretical Background and Practice." Postgraduate Medical Journal 63, no. 736 (February 1, 1987): 158. http://dx.doi.org/10.1136/pgmj.63.736.158.

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Hakso-Mäkinen, Heli, and Ivana Kholová. "New Cell Block Method to Enhance the Cellular Yield in Mucous and/or Bloody Samples." Acta Cytologica 64, no. 3 (August 30, 2019): 265–69. http://dx.doi.org/10.1159/000501817.

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Objective: Cell blocks (CBs) are used to complement cytological diagnosis and for ancillary testing. Dissatisfaction with the cellular yield of the CB is widely recognized. Various techniques have been developed to increase the diagnostic utility of CBs. Study Design: We invented a new CB technique to increase cellular yield and diagnostic accuracy suitable especially for mucous and/or bloody cytological samples. Results: The new CB technique is described in detail with illustrations and cases, where it increased the cellular yield and diagnostic accuracy. CBs prepared by this method are suitable also for ancillary techniques, namely immunocytochemistry. Conclusions: The newly described method showed a better cellular yield in mucous and/or bloody cytological specimens.
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Cecchini Gualandi, Stefano, Tommaso Di Palma, and Raffaele Boni. "Serological and Uterine Biomarkers for Detecting Endometritis in Mares." Animals 13, no. 2 (January 11, 2023): 253. http://dx.doi.org/10.3390/ani13020253.

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Serological analysis may provide relevant information on endometritis diagnostics. Therefore, mares scheduled for AI with refrigerated semen, at the time of heat signs, underwent blood and uterine fluid samplings using a swab, uterine lavage for culture analysis, and treatment with human chorionic gonadotropin to induce ovulation. After 24–28 h, the mares were inseminated and, if positive at the culture test, treated with antibiotics chosen based on the susceptibility test. Uterine cells obtained by swabs were used for cytological examination with both classical and fluorescence techniques. Blood serum and uterine fluid samples were analyzed for assessing parameters related to redox balance, inflammation, and protease regulator potential. In blood serum, total antioxidant capacity, measured as the ferric reducing ability of plasma (FRAP), was significantly lower in cytologically endometritis-positive than -negative mares. In the uterine fluid, total thiol levels (TTL), nitric oxide metabolites (NOx), protease activity and total protein content varied significantly between groups. Although the cytological examination was more capable of discriminating between endometritis-positive and -negative mares in relation to the parameters examined, no statistically significant differences emerged in terms of pregnancy rate in relation to cytological and culture diagnosis as well as in mares diagnosed as positive and negative for endometritis.
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Krogerus, Leena, and Ivana Kholová. "Cell Block in Cytological Diagnostics: Review of Preparatory Techniques." Acta Cytologica 62, no. 4 (2018): 237–43. http://dx.doi.org/10.1159/000489769.

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Objective: The cell block (CB) technique refers to the processing of sediments, blood clots, or grossly visible tissue fragments from cytological specimens into paraffin blocks that can be cut and stained by the same methods used for histopathology. The technique brings additional tissue architectural information. CB can be used for ancillary techniques such as immunocytochemistry and molecular techniques. Study Design: We reviewed the literature on the various preparatory techniques of CBs. Results: There is a wide range of preparatory techniques for CBs and no golden standard for CBs exists: tens of methods are used in various institutions. The majority of the methods are modified in house techniques with a few commercially available kits. The techniques most commonly used are the plasma/thrombin method, the agar method, and commercially available Histogel- and Cellient CB-methods. Dissatisfaction with the cellular yield of the CBs is common. Conclusions: In the CBs, the cytological material is preserved for future use, which is a tremendous advantage in the era of targeted therapy and biobanking. The CB is thus central to the future of cytology: more can be done with less material and with less invasiveness to the patient.
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Kaptilnyy, V. A. "Taking ecto- and endocervical scrapings for the cytological study." V.F.Snegirev Archives of Obstetrics and Gynecology 3, no. 2 (June 15, 2016): 92–96. http://dx.doi.org/10.18821/2313-8726-2016-3-2-92-96.

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In the article there is presented the technique of taking ecto- and endocervical scrapings for the cytological method of research. There are described in details the various techniques used in routine clinical practice. Particular attention is paid to the quality of the collecting cytological sample - technique of taking samples of cellular material. There is described the preparation to the study, readings and research goals. The accompanying photos illustrate in detail all the stages of the cytological screening of the uterine cervix.
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MUGALE, MADHAV, VIKASH SINGH, DNYANESHWAR GAVHANE, and GAGAN GOSWAMI. "Salient features of sticker tumour in dogs and its diagnosis by cytopathology and histopathology technique." Indian Journal of Animal Sciences 84, no. 4 (April 16, 2014): 379–81. http://dx.doi.org/10.56093/ijans.v84i4.39834.

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Six dogs of various ages, breeds and sex showed tumourous growth confined to extragenital regions. The study was aimed at diagnosing tumourous growth using routine technique clinically and pathologically. Cytological techniques and later the results were compared with routine histopathology. Two male Labrador dogs, 1 female Spitz and 3 male non-descript dogs with tumour masses over and around the genital organ were used. Tumour impression sample and excised tumour were used as material for the study. Fine needle aspiration cytopathology (FNAC) with various cytological stains and routine histopathology with haematoxylin and eosin staining were performed. Grossly, the tumour masses appeared as single or multiple irregular, cauliflower like and had a tendency to bleed and in almost all cases colour was pink to red. Cytologically, the tumour yielded a homogenous, sheet-like high cellular mass. Cytoplasm with punctate vacuoles, anisokaryosis with anisonucleoliosis and coarse to reticulate nuclear chromatin were prominent features. Histopathology showed sheets of round cells with nuclear and cytoplasmic variations. The study concluded that cytopathology could be used as a quick, rapid, field diagnostic technique in combination with histopathology for the diagnosis of TVTs.
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Mendes, Sarah Freygang, Grasieli de Oliveira Ramos, Elena Riet Correa Rivero, Filipe Modolo, Liliane Janete Grando, and Maria Inês Meurer. "Techniques for Precancerous Lesion Diagnosis." Journal of Oncology 2011 (2011): 1–5. http://dx.doi.org/10.1155/2011/326094.

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The development of the oral squamous cell carcinoma (OSCC) is a multistep process that requires the accumulation of multiple genetic alterations usually preceded by detectable mucosal changes, most often leukoplakias and erythroplakias. The clinical appearance of oral precancerous lesions and their degree of epithelium dysplasia suggests the malignization potential. Several techniques have been developed to improve the clinical and cytological diagnosis of oral precancerous lesions. The present paper reviews the main techniques used to improve premalignant lesion diagnosis.
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Dissertations / Theses on the topic "Cytological techniques"

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Wang, Naining. "Quantitative cellular methods in the evaluation of prostate cancer /." Stockholm, 2000. http://diss.kib.ki.se/2000/91-628-3929-2/.

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BEOLCHI, RAFAEL da S. "Adicao de complexo vitaminico em duas bioceramicas e seu efeito na regeneracao ossea." reponame:Repositório Institucional do IPEN, 2009. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11529.

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IPEN/D
Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP
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Santos, Fernanda Erci dos. ""Identificação do papilomavírus humano em gestantes adolescentes por meio da captura hibrida II:correlação com a colpocitologia oncótica convencional, em base líquida e colposcopia"." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/5/5139/tde-26052006-120533/.

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Estudo prospectivo para identificar a presença do papilomavírus humano em gestantes adolescentes por meio da captura híbrida II e correlacionar com colpocitologia oncótica convencional, em base líquida e colposcopia. O grupo constituído por 60 gestantes entre 12 a 18 anos e idade gestacional media de 23 semanas. A captura híbrida II foi positiva em 51,7%. A colpocitocologia oncótica convencional : normal em 90% e anormal em 10%. Os achados citológicos anormais: lesão intraepitelial escamosa de baixo grau em 8,3% e carcinoma invasor em 1,7%. A colpocitologia em base líquida: normal em 90% e anormal em 10%. Os achados citológicos anormais: lesão intraepitelial escamosa de baixo grau em 8,3% e lesão intraepitelial escamosa de alto grau em 1,7%. Os achados colposcópicos normais foram o epitélio escamoso normal em 20%, epitélio glandular em 18,3% e a zona de transformação normal em 40%. A zona de transformação anormal presente em 21,7%
Study delineated to identify human papillomavirus by hybrid capture II in pregnant teenagers and to correlate with conventional, liquid-based Pap test and colposcopic findings. The study group was constituted by 60 pregnant women aged between 12 and 18 years old; mean gestational age was 23 weeks. They were submitted to anamnese, Pap smear, hybrid capture technique and colposcopy. Hybrid capture II of human papillomavirus was positive in 51,7% of the cases. Conventional Pap test was normal in 90% and abnormal in 10% of the cases. Abnormal results: low-grade squamous intraepithelial lesion in 8,3% and Invasor carcinoma in 1,7%. Based-liquid Pap test was normal in 90% and abnormal in 10% of the cases. Abnormal results: 8,3% of low-grade squamous intraepithelial lesion and 1,7% of high-grade squamous intraepithelial lesion. Normal results: 20% of normal squamous epithelium, 18,3%of columnar epithelium and 40% of normal transformation zone. Abnormal transformation zone was seen in 21,7% of the cases
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MARTINI, GISELA de A. "Avaliação da citotoxicidade do Dietiltoluamida (DEET) em mexilhões Perna perna (Linnaeus, 1758) irradiados e não irradiados com radiação gama de sup(60)Co." reponame:Repositório Institucional do IPEN, 2013. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10579.

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IPEN/D
Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP
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Fraga, Thalyta Porto. "Análise citopatológica do líquor em pacientes com leucemia linfoblástica aguda." Pós-Graduação em Ciências da Saúde, 2018. http://ri.ufs.br/jspui/handle/riufs/8898.

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Introduction: The Central Nervous System (CNS) is an important site of relapse in patients with Acute Lymphoblastic Leukemia (ALL), despite increasing cure rates in the last four decades in young patients. The presence of leukemic blasts in the Cerebrospinal Fluid (CSF) presents prognostic implication and therefore defines changes in the therapeutic protocol, which is why it requires diagnostic accuracy. The emergence of new techniques for identifying CSF leukemia blasts, such as flow cytometry and molecular methods, which are more costly and not uniformly accessible for clinical use, have led to the need to reassess the role of conventional cytology as diagnostic tool. Objectives: To identify the proportion of CSF samples positive for blasts in children and adolescents with ALL, using a standardized cytology technique and with standardization of variables related to the process that could interfere with the result. DESIGN: Prospective, descriptive, uncontrolled study in which samples of CSF obtained by lumbar puncture of patients with ALL that were starting treatment were examined. CSF samples were sent to the laboratory shortly after collection, being processed and cytocentrifugated in cytofunyl in up to four hours after collection. Four slides were prepared, stained and analyzed by a pathologist and a hematologist. RESULTS: Twenty-eight patients with ALL were evaluated, with predominance of male (58.6%), immunophenotype B (82.2%) and 78.5% were stratified as high risk for relapse. Of the 205 CSF samples evaluated, 26 (12.6%) were positive for blasts and among 28 patients, 11 (39.2%) had CSF with neoplastic infiltration. Comparing the groups with and without CNS infiltration, no statistically significant difference was observed for the variables analyzed. CONCLUSIONS: Conventional cytology was effective in the identification of CNS infiltration by blasts, provided there is a vigilance of factors related to collection, processing and analysis of CSF that may interfere in the reliability of the result.
Introdução: O Sistema Nervoso Central (SNC) é importante sítio de recaída em pacientes com Leucemia Linfoblástica Aguda (LLA), apesar das crescentes taxas de cura nas últimas quatro décadas em pacientes jovens. A presença de blastos leucêmicos no Líquido Cefalorraquiano (LCR) apresenta implicação prognóstica e, portanto, define mudanças no protocolo terapêutico, razão pela qual requer acurácia diagnóstica. O surgimento de novas técnicas de identificação de blastos leucêmicos no LCR, como a citometria de fluxo e os métodos moleculares, que têm custo mais elevado e não estão uniformemente acessíveis para uso clínico, trouxe a necessidade de reavaliar-se o papel da citologia convencional como instrumento diagnóstico. Objetivos: Identificar a proporção de exames de LCR positivos para blastos em crianças e adolescentes com LLA, utilizando-se de técnica de citologia padronizada e com padronização de variáveis relacionadas ao processo que pudessem interferir no resultado. Delineamento: Estudo prospectivo, descritivo, não controlado, no qual foram examinadas amostras de LCR obtidas por punção lombar de pacientes com LLA que estavam iniciando o tratamento. As amostras de LCR foram encaminhadas ao laboratório logo após a coleta, sendo processadas e citocentrifugadas em citofunil em até no máximo quatro horas após a coleta. Quatro lâminas foram preparadas, coradas e analisadas por um patologista e um hematologista. Resultados: Foram avaliados 28 pacientes com LLA, havendo predomínio do sexo masculino (58,6%), imunofenótipo B (82,2%) e 78,5% foram estratificados como de alto risco para recaída. Dentre as 205 amostras de LCR avaliadas, 26 (12,6%) foram positivas para blastos e dentre os 28 pacientes, 11 (39,2%) obtiveram algum exame de LCR com infiltração neoplásica. Comparando-se os grupos com e sem infiltração de SNC, não se observou diferença estatisticamente significante para as variáveis analisadas. Conclusão: Citologia convencional foi efetiva na identificação de infiltração de SNC por blastos leucêmicos, desde que haja vigilância dos fatores relacionados a coleta, processamento e análise do LCR que possam interferir na fidedignidade do resultado.
Aracaju, SE
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Renesto, Adimara da Candelaria [UNIFESP]. "Efeito da riboflavina tópica exposta à irradiação ultravioleta A e inserção de segmentos de anéis corneanos intraestromais para ceratocone." Universidade Federal de São Paulo (UNIFESP), 2012. http://repositorio.unifesp.br/handle/11600/9359.

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Objetivos: 1) Avaliar se o cross-linking do colágeno corneano antes da inserção de segmentos de anéis corneanos intraestromais altera o tratamento do ceratocone. 2) Avaliar se o cross-linking do colágeno corneano modifica as características citológicas da superfície ocular em portadores de ceratocone. Métodos: Trinta e nove olhos de 31 pacientes foram alocados em 2 grupos: 19 olhos foram submetidos ao tratamento do cross-linking com riboflavina e luz ultravioleta A, e 20 olhos receberam colírio de riboflavina 0,1% (w/v) em solução de 20% dextran 4 vezes ao dia por 30 dias. Após 3 meses, todos os pacientes foram submetidos à inserção de segmentos de anéis corneanos intraestromais pela técnica do laser de femtosegundo. Avaliações foram realizadas nos momentos pré-operatório, com 1 e 3 meses após o cross-linking ou colírio de riboflavina, e também com 1, 3, 6, 12 e 24 meses após a inserção de segmentos de anéis corneanos intraestromais. Os pacientes foram submetidos aos seguintes exames: acuidade visual sem correção e acuidade visual com correção; refração manifesta; biomicroscopia; sensibilidade ao contraste; topografia corneana; Orbscan IIz; Pentacam; tonometria de aplanação; tonometria dinâmica de contorno; paquimetria ultrassônica; biomecânica da córnea; tomografia de coerência óptica; microscopia especular da córnea; citologia de impressão e mapeamento de retina. Análise de covariância e o teste de Mann-Whitney foram realizados para comparação das variáveis do estudo entre os 2 grupos. Teste de Friedman foi utilizado para avaliação da citologia de impressão antes do tratamento, e com 1 e 3 meses após o cross-linking ou colírio de riboflavina, e novamente com 6, 12 e 24 meses após a inserção de segmentos de anéis corneanos intraestromais. Resultados: Todos os pacientes foram acompanhados por 24 meses. A média e variação de idade dos pacientes foram de 28,30 ± 9,3 anos (17-55 anos) no grupo cross-linking e 30,40 ± 9,1 anos (22-55 anos) no grupo colírio de riboflavina. Não houve diferença estatisticamente significante entre os grupos em relação à acuidade visual sem correção (p=0,70) ou acuidade visual corrigida (p=0,78). Com 24 meses de seguimento, não houve diferença estatística entre os grupos em relação ao equivalente esférico (p=0,94). Também não houve diferença estatística para os 3 parâmetros topográficos (mais plano-K1 [p=0,81], mais curvo-K2 [p=0,68] e curvatura média [p=0,52]). Os exames de sensibilidade ao contraste, microscopia especular, paquimetria, tonometria e propriedades biomecânicas da córnea não apresentaram diferença estatística entre os grupos com 24 meses de seguimento. Na citologia de impressão, alguns parâmetros conjuntivais (ex.: adesividade das células epiteliais, proporção núcleo:citoplasma, nível de organização da cromatina nuclear, células caliciformes e queratinização [p≥0,001]) apresentaram diferenças estatisticamente significantes. Conclusões: O cross-linking do colágeno corneano não altera o efeito de segmentos de anéis corneanos intraestromais para ceratocone antes de sua inserção em relação à refração, topografia, sensibilidade ao contraste, microscopia especular, paquimetria, tonometria e biomecânica da córnea com 24 meses de seguimento. A comparação do escore total de citologia de impressão entre os grupos não mostrou diferença estatisticamente significante, apesar de diferenças em alguns parâmetros conjuntivais.
Purpose: To report the impression cytologic results after corneal cross-linking and insertion of intrastromal corneal ring segments for keratoconus. Methods: Thirty-nine eyes were distributed into two groups: 1) cross-linking group (patients underwent corneal cross-linking procedure), and 2) riboflavin eyedrops group (patients received riboflavin 0.1% (w/v) eyedrops in 20% dextran solution for 1 month). After 3 months, all patients underwent insertion of intrastromal corneal ring segments. Impression cytologic specimens were obtained from all eyes at baseline, at 1 month and 3 months after cross-linking or riboflavin eyedrops, and again at 6 months, 1 year, and 2 years after intrastromal corneal ring segment insertion. Results: Patients in the cross-linking group demonstrated improvement in the cell-to-cell contact of epithelial cells and the nucleusto- cytoplasm ratio on the temporal conjunctiva after treatment (P = 0.008 and P = 0.047), respectively. On the superior conjunctiva, increases in goblet cell density (P = 0.037) and level of organization of nuclear chromatin (P = 0.010) after treatment were noted. Patients in the riboflavin eyedrops group demonstrated improvement in the cellto- cell contact of epithelial cells on the superior conjunctiva after treatment (P = 0.021). On the temporal conjunctiva, an improvement in the cell-to-cell contact of epithelial cells (P < 0.001) and increases in the nucleus-to-cytoplasm ratio (P < 0.001), goblet cell density (P = 0.001), and less keratinization (P = 0.011) were noted. No changes were identified on the cornea for either group. Fisher’s exact test comparison of the impression cytologic total scores after treatment revealed no difference between groups. Conclusion: Despite changes in some conjunctival parameters (e.g., cell-tocell contact of epithelial cells, nucleus-to-cytoplasm ratio, level of organization of nuclear chromatin , goblet cell density, and keratinization), comparison of the total impression cytologic scores revealed no difference between groups.
TEDE
BV UNIFESP: Teses e dissertações
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Persson, Anders. "Affinity partitioning of membranes purification of rat liver plasma membranes and localization of phosphatidylinositol 4-kinase /." Lund : Biochemistry, Chemical Center, University of Lund, 1995. http://books.google.com/books?id=BqpqAAAAMAAJ.

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Nahas, Caio Sergio Rizkallah. "Rastreamento da displasia anal em pacientes infectados pelo HIV: há concordância entre o estregaço anal e a biópsia guiada por anuscopia de alta resolução?" Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/5/5154/tde-10072012-145651/.

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OBJETIVO: O objetivo deste estudo foi analisar a concordância entre o esfregaço anal e a biópsia guiada por anuscopia de alta resolução no diagnóstico da displasia anal em pacientes infectados pelo HIV. MÉTODO: Conduzimos uma análise transversal de pacientes infectados pelo HIV submetidos a rastreamento de displasia anal rotineiro. A concordância entre mensurações foi estimada por índice de kappa ponderado através de sistema de avaliação citológica e histológica de três categorias (normal, displasia de baixo grau, e displasia de alto grau). Estimativas de sensibilidade, especificidade e valores preditivos foram calculados através de sistema de avaliação citológica e histológica de duas categorias (ausência de displasia e displasia de qualquer grau). Estimativas foram calculadas também para a detecção de displasia de alto grau. RESULTADOS: No decorrer de um ano, 222 pacientes foram submetidos a 330 esfregaços anais seguidos de biópsias guiadas por anuscopia de alta resolução. Trezentos e onze (311) esfregaços com biópsias concomitantes foram satisfatórios. Considerando-se a histologia como padrão, a freqüência de displasia anal foi de 46%. O índice kappa ponderado para concordância entre o esfregaço anal e a biópsia foi de 0,20. Para detecção de displasia anal de qualquer grau, o esfregaço anal demonstrou sensibilidade de 61%, especificidade de 60%, valor preditivo positivo de 56% e valor preditivo negativo de 64%. Para displasia de alto grau, o esfregaço anal demonstrou sensibilidade de 16% e especificidade de 97%. CONCLUSÃO: Os resultados obtidos no presente estudo, em que comparamos os achados da citologia dos esfregaços com os achados histológicos das biópsias dirigidas pela anuscopia de alta resolução em pacientes infectados pelo HIV permitiram concluir que houve baixa concordância entre eles
Purpose: To analyze the agreement between anal Pap smear and high resolution anoscopy guided biopsy to diagnose anal dysplasia in HIV-infected patients. Methods: Cross sectional analysis of HIV-infected patients receiving anal dysplasia screening as part of routine care. Agreement between measures was estimated by weighted kappa-statistics, using 3-tiered cytologic and histologic grading system (normal, low grade dysplasia, and high grade dysplasia). Estimates of sensitivity, specificity, and predictive values were calculated using a 2-tiered cytologic and histologic grading system (without dysplasia, and with dysplasia of any grade). Estimates were also calculated for the detection of high grade dysplasia. Results: Two hundred and twenty-two patients underwent 330 anal Pap smears followed by high resolution anoscopy guided biopsies in one year period. There were 311 satisfactory Pap smears with concurrent biopsy. Considering histology the standard, the frequency of anal dysplasia was 46 percent (95 percent confidence interval: 40-51 percent). Kappa-agreement between anal Pap smear and biopsy was 0.20 (95 percent confidence interval: 0.10 0.29). Anal Pap smear showed sensitivity of 61 percent, specificity of 60 percent, positive predictive value of 56 percent, and negative predictive value of 64 percent for detection of anal dysplasia of any grade. For high grade dysplasia, anal Pap smear showed sensitivity of 16 percent, and specificity of 97 percent. Conclusion: The present study showed a low concordance between anal Pap smears and high resolution anoscopy-guided biopsy
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Vieira, Elizabeth Dell Orto. "PrevalÃncia de HPH e lesÃes intraepiteliais escamosas em gestantes." Universidade Federal do CearÃ, 2008. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=1179.

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CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior
Estudo de prevalÃncia (estudo transversal) sobre infecÃÃo por HPV em colo uterino de gestantes, avaliando o(s) subgrupo(s) de HPV mais prevalentes e a associaÃÃo quanto ao desenvolvimento de lesÃes intraepiteliais escamosas. A metodologia constituiu-se de questionÃrio aplicado diretamente a 272 gestantes, independente da idade gestacional e de estarem sintomÃticas ou nÃo na primeira consulta de prÃ-natal, alÃm da coleta de material cÃrvico-vaginal (paredes vaginais, ectocÃrvice e endocÃrvice) para realizaÃÃo de Citologia oncÃtica convencional pelo mÃtodo de coloraÃÃo proposto por Papanicolaou e do teste de Captura HÃbrida IIÂ. Procedeu-se a seguir a identificaÃÃo das lesÃes intraepiteliais escamosas e da presenÃa do vÃrus HPV de alto, baixo e mÃdio risco oncogÃnico. Os achados da Captura HÃbrida IIÂ foram correlacionados com os achados citolÃgicos, comparando-se a freqÃÃncia dos resultados anormais nos dois mÃtodos. Procedeu-se tambÃm a associaÃÃo com aspectos biossociais que pudessem interferir na infecÃÃo produzida pelo HPV e no possÃvel desenvolvimento de lesÃes intraepiteliais escamosas. Realizaram-se anÃlises de correlaÃÃo univariada e bivariada, com cÃlculo do Qui-quadrado de Pearson e valor p< 0,05. Utilizou-se tambÃm de regressÃo logÃstica para estimar a magnitude das associaÃÃes. A prevalÃncia de infecÃÃo por HPV e citologias oncÃticas anormais foi de 32,3% e 14,0 % respectivamente. Os subgrupos de HPV de alto risco foram mais prevalentes (27,6%) do que os de baixo e mÃdio risco (18,4%) e a sua associaÃÃo mostrou-se estatisticamente significativa (p<0,05). A associaÃÃo da infecÃÃo genital por HPV de alto risco com lesÃes intraepiteliais escamosas descritas na Citologia oncÃtica convencional foi mais prevalente (8,5%) do que com HPV de baixo de mÃdio risco (5,6%) e teve significÃncia estatÃstica (p<0,05)
This work studies the prevalence (cross sectional) on infection by HPV in uterine cervical of pregnant women evaluating the subgroups of HPV more prevalents and the association in relation to the development of squamous intraepithelial lesions. The methodology was constituted of questionary applied to 272 pregnant women independent of pregnant age and of being symptomatic or not in the first medical advice of prenatal besides the collect of cervico vaginal material (vaginal walls, ectocervix and endocervix) to the achievement of conventional oncotic cytology by the method of coloration proposed by Papanicolaou and the test of Capture Hybrid IIÂ. Then it was proceeded the identification of squamous intraepithelial lesions and the presence of HPV virus of high, low and medium oncogenic risk. The results of Capture Hybrid IIÂ were correlated with the cytologic discoveries comparing the frequency of anormal results in both methods. It was also proceeded the association with biosocial aspects which could interfere in the possible development of squamous intraepithelial lesions. The analysis of univariable and bivariable correlations were accomplished with calculus of Qui-squared of Pearson and value p<0,05. The logistic regression was used to estimate the importance of associations. The prevalence of infection by HPV and anormal oncotic cytologies was about 32,3% and 14,0% respectively. The sub groups of HPV of high risk were more prevalent (27,6%) than the ones of low and medium risk. (18,4%) and their association showed to be statistically significant (p< 0,05). The association of genital infection by HPV of high risk with squamous epithelial lesions described on conventional oncotic cytology was more prevalent (8,5%) than with HPV of low and medium risk (5,6%) and had statistical importance (p< 0,05)
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"Telecytopathology with mainland China." 2002. http://library.cuhk.edu.hk/record=b5891356.

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Yuan, Qin.
Thesis (M.Phil.)--Chinese University of Hong Kong, 2002.
Includes bibliographical references (leaves 112-119).
Abstracts in English and Chinese.
Acknowledgements --- p.III
Publications --- p.V
List of Abbreviations --- p.VI
List of Figures --- p.VII
List of Tables --- p.VIII
Abstract in English --- p.IX
Abstract in Chinese --- p.XII
Table of Contents --- p.XIII
Chapter CHAPTER1 I --- NTRODUCTION --- p.1
Chapter 1.1 --- What is telemedicine --- p.1
Chapter 1.2 --- What is telepathology --- p.4
Chapter 1.2.1 --- Types of telepathology system --- p.7
Chapter 1.2.2 --- Main events in the development of telepathology --- p.9
Chapter 1.2.3 --- Diagnostic accuracy in telepathology and telecytology --- p.12
Chapter 1.2.4 --- Applications of telepathology --- p.18
Chapter 1.2.4.a --- Remote primary diagnosis --- p.18
Chapter 1.2.4.b --- Remote expert consultation --- p.19
Chapter 1.2.4.c --- "Image libraries, databases and archiving" --- p.20
Chapter 1.2.4.d --- Quality assurance --- p.20
Chapter 1.2.4.e --- Remote teaching and training --- p.21
Chapter 1.3 --- Technical aspects of telepathology --- p.24
Chapter 1.3.1 --- Image presentation --- p.24
Chapter 1.3.2 --- Image acquisition and display --- p.25
Chapter 1.3.3 --- Image compression --- p.27
Chapter 1.3.4 --- Networking and line connections --- p.29
Chapter 1.3.4.a --- Public (analog) telephone network --- p.29
Chapter 1.3.4.b --- Integrated services digital network (ISDN) --- p.30
Chapter 1.3.4.c --- Computer network --- p.30
Chapter 1.3.4.d --- Asynchronous transfer mode (ATM) --- p.31
Chapter 1.4 --- Legal and ethical aspects --- p.32
Chapter 1.4.1 --- Licensure of the facility --- p.33
Chapter 1.4.2 --- Licensure of the pathologist --- p.33
Chapter 1.4.3 --- Accreditation --- p.34
Chapter 1.4.4 --- The electronic medical record: privacy --- p.35
Chapter 1.4.5 --- Malpractice liability --- p.36
Chapter 1.4.6 --- Reimbursement --- p.38
Chapter 1.4.7 --- Conclusion --- p.38
Chapter 1.5 --- Telemedicine and telepathology in China --- p.40
Chapter 1.6 --- Cytopathology practice in China --- p.42
Chapter CHAPTER2 --- OBJECTIVES OF STUDY --- p.46
Chapter CHAPTER3 --- MATERIALS AND METHODS --- p.48
Chapter 3.1 --- Case materials --- p.48
Chapter 3.2 --- Static image capture and display --- p.51
Chapter 3.3 --- Static telecytology study --- p.55
Chapter 3.4 --- Web-based tutorial program --- p.59
Chapter 3.4.1 --- Pre-tutorial evaluation --- p.60
Chapter 3.4.2 --- Cytology tutorial --- p.62
Chapter 3.4.3 --- Post-tutorial evaluation --- p.66
Chapter 3.4.4 --- Data analysis --- p.67
Chapter 3.5 --- Dynamic telecytology study --- p.68
Chapter 3.5.1 --- Equipment --- p.68
Chapter 3.5.2 --- Trial design --- p.72
Chapter 3.5.2a --- Telecytology diagnosis --- p.72
Chapter 3.5.2b --- Light microscopy diagnosis --- p.73
Chapter 3.5.2c --- Data analysis --- p.74
Chapter CHAPTER4 --- RESULTS --- p.75
Chapter 4.1 --- General information about participating cytology laboratories --- p.75
Chapter 4.2 --- Static telecytology study --- p.78
Chapter 4.2.1 --- Telecytology diagnostic agreement --- p.78
Chapter 4.2.2 --- Confidence of telecytology diagnosis --- p.80
Chapter 4.2.3 --- "The acceptance of the image quality, time required for each case" --- p.83
Chapter 4.3 --- Web-based program on cervical cytology --- p.84
Chapter 4.4 --- Dynamic telecytology study --- p.86
Chapter 4.4.1 --- Diagnostic accuracy --- p.86
Chapter 4.4.2 --- Time studies --- p.88
Chapter 4.4.3 --- Diagnostic certainty --- p.89
Chapter 4.4.4 --- Image quality --- p.91
Chapter CHAPTER5 --- DISCUSSION --- p.92
Chapter 5.1 --- Static image telecytology study linking 14 Mainland China hospitals --- p.93
Chapter 5.2 --- Problems encountered in setting up links with Mainland China --- p.97
Chapter 5.3 --- Web-based tutorial programme for remote teaching --- p.100
Chapter 5.4 --- Dynamic image telecytology study for immediate diagnosis at a distance --- p.103
Chapter 5.5 --- Limitations of this study --- p.105
Chapter 5.6 --- Concluding remarks --- p.108
REFERENCES --- p.112
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Books on the topic "Cytological techniques"

1

Boon, Mathilde E., and Johanna S. Drijver. Routine Cytological Staining Techniques. London: Macmillan Education UK, 1986. http://dx.doi.org/10.1007/978-1-349-18250-3.

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Jong, Kwiton. Laboratory manual of plant cytological techniques. Edinburgh: Royal Botanic Garden, 1997.

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S, Drijver Johanna, ed. Routine cytological staining techniques: Theoretical background and practice. Basingstoke, Hampshire: Macmillan, 1986.

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Boon, Mathilde E. Routine cytological staining techniques: Theoretical background and practice. Basingstoke: Macmillan, 1985.

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1939-, Bibbo Marluce, ed. Comprehensive cytopathology. 2nd ed. Philadelphia: Saunders, 1997.

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Cox, Guy. Optical imaging techniques in cell biology. 2nd ed. Boca Raton: Taylor & Francis/CRC Press, 2012.

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Celis, J. E., and Geri Kreitzer. Cell biology assays: Essential methods. London: Academic, 2009.

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Mary, Hannon-Fletcher, and Maxwell Perry 1958-, eds. Advanced techniques in diagnostic cellular pathology. Chichester, West Sussex: John Wiley & Sons, 2009.

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Klaus, Goerttler, Feichter G. E. 1946-, and Witte Siegfried, eds. New frontiers in cytology: Modern aspects of research and practice. Berlin: Springer-Verlag, 1988.

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John, Chad, and Wheal H. V, eds. Cellular neurobiology: A practical approach. Oxford: IRL Press at Oxford University Press, 1991.

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Book chapters on the topic "Cytological techniques"

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Negrutiu, I., and G. B. Gharti-Chhetri. "Cytological Techniques." In A Laboratory Guide for Cellular and Molecular Plant Biology, 287–363. Basel: Birkhäuser Basel, 1991. http://dx.doi.org/10.1007/978-3-0348-7502-8_5.

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Karp, Angela. "Cytological techniques." In Plant Tissue Culture Manual, 503–15. Dordrecht: Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-009-0103-2_28.

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Boon, Mathilde E., and Johanna S. Drijver. "Preparing Cytological Slides." In Routine Cytological Staining Techniques, 102–20. London: Macmillan Education UK, 1986. http://dx.doi.org/10.1007/978-1-349-18250-3_10.

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Banks, R. W. "Cytological Staining Methods." In Modern Techniques in Neuroscience Research, 1–26. Berlin, Heidelberg: Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-642-58552-4_1.

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Khasim, S. M., K. Thammasiri, S. Rama Rao, and M. Rahamtulla. "Cytological and Vital Staining Techniques." In Plant Techniques, 341–71. London: CRC Press, 2024. http://dx.doi.org/10.1201/9781003503682-19.

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Boon, Mathilde E., and Johanna S. Drijver. "Cell Biology." In Routine Cytological Staining Techniques, 3–15. London: Macmillan Education UK, 1986. http://dx.doi.org/10.1007/978-1-349-18250-3_1.

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Boon, Mathilde E., and Johanna S. Drijver. "Comparing Cells in Histology and Cytology." In Routine Cytological Staining Techniques, 16–32. London: Macmillan Education UK, 1986. http://dx.doi.org/10.1007/978-1-349-18250-3_2.

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Boon, Mathilde E., and Johanna S. Drijver. "Principles of Staining and Dyeing." In Routine Cytological Staining Techniques, 33–50. London: Macmillan Education UK, 1986. http://dx.doi.org/10.1007/978-1-349-18250-3_3.

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Boon, Mathilde E., and Johanna S. Drijver. "Fixation." In Routine Cytological Staining Techniques, 51–59. London: Macmillan Education UK, 1986. http://dx.doi.org/10.1007/978-1-349-18250-3_4.

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Boon, Mathilde E., and Johanna S. Drijver. "Nuclear Dyes." In Routine Cytological Staining Techniques, 60–67. London: Macmillan Education UK, 1986. http://dx.doi.org/10.1007/978-1-349-18250-3_5.

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Conference papers on the topic "Cytological techniques"

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"AUTOMATED COMBINED TECHNIQUE FOR SEGMENTING CYTOLOGICAL SPECIMEN IMAGES." In Mathematical and Linguistic Techniques for Image Mining. SciTePress - Science and and Technology Publications, 2007. http://dx.doi.org/10.5220/0002071402380245.

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Emilian, Onisan, Petrescu Irina, Sarac Ioan, Camen Dorin, and Stroia Ciprian. "CYTOLOGICAL DIAGNOSTIC TECHNIQUES FOR ENHANCING RESILIENCE IN BREEDING PROGRAMS." In 23rd SGEM International Multidisciplinary Scientific GeoConference 2023. STEF92 Technology, 2023. http://dx.doi.org/10.5593/sgem2023v/6.2/s25.14.

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Since the inception of modern plant breeding, especially with the discovery of heterosis and the development of hybrids, which are known for their ability to enhance crop yields, plant breeders have consistently strived to advance and identify an efficient method for developing inbred lines. In this endeavor, breeders have encountered various challenges, including the complexity of requiring multiple generations to develop inbred lines and the struggle to find effective ways to optimize this process. In our research, we aim to present a method for cytological diagnosis and the identification of high levels of inbreeding. Our study involves the analysis of sunflower genotypes, both hybrids and inbred lines, regarding the mitotic index and its correlation with leaf area and root development in an automatic rhizotron. During our research, we observed significant differences between hybrid genotypes and inbred lines in terms of the mitotic index and variations in leaf area and root development. Therefore, this research provides insight into the applicability of cytology for timely identification and elimination of inbred genetic material, thereby contributing to increased resilience and economic efficiency in breeding programs through the adoption of cytological diagnostic techniques.
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"AN APPLICATION OF A DESCRIPTIVE IMAGE ALGEBRA FOR DIAGNOSTIC ANALYSIS OF CYTOLOGICAL SPECIMENS - An Algebraic Model and Experimental Study." In Mathematical and Linguistic Techniques for Image Mining. SciTePress - Science and and Technology Publications, 2007. http://dx.doi.org/10.5220/0002071502300237.

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Adany, R., A. Kiss, J. Kappelmayer, R. J. Ablin, and L. Muszbek. "EXPRESSION OF FACTOR XIII SUBUNIT A IN DIFFERENT TYPES OF HUMAN MACROPHAGES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644651.

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In addition to plasma the presence of subunit a of blood coagulation Factor XIII (FXIIl) has been verified in platelets and megakariocytes. Most recently, we demonstrated that human peripheral blood monocytes also contain FXIII subunit a. The present study was designed 1/ to determine the stage in the maturation sequence of bone marrow monocytopoesis in which FXIII appears 2/ to establish if FXIII is retained during differentiation into macrophages 3/ to assess how general is the presence of FXIII subunit a in different types of macrophages. FXIII subunit a was immunomorphologically detected in bone marrow smears, in cytospin preparations of cells from serous cavities (pleural, peritoneal, pericardial and synovial spaces), and paraformaldehyde-fixed paraffin-embedded or frozen sections of different organs where classical types of macrophages have been described earlier (liver, lung, thymus, skin, connective tissue, prostate and developing bone) . Cells containing FXIII subunit a were intensively characterized by immunofluorescent and enzymecytochemical techniques in double and treble labeling systems. Its presence was clearly demonstrated in promonocytes of bone marrow, and in all probability, it is present in monoblasts, as well. FXIII was also found in macrophages from different serous cavities and in embryonic osteoclasts. Cells containing FXIII subunit a of connective tissue were found to be tissue histiocytes, and not fibroblasts as previously thought. Kupffer cells of the liver and Langerhans cells of the epidermis were negative supporting theories that these cells are not members of monocyte-derived macrophage cell population. Immunomorphological detection of FXIII subunit a seems to be a useful marker for labeling the continuum of monocyte/macrophage cell line from the earliest ftrais in the bone marrow to the mature forms of macrophages and might be a valuable tool in the cytological diagnosis of malignant disorders of this cell line.
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Herman, B. "APPLICATIONS OF LASER OPTICAL MICROSCOPIC TECHNIQUES IN DECIPHERING DISEASE SPECIFIC MECHANISMS AND DIAGNOSIS." In Biomedical Optical Spectroscopy and Diagnostics. Washington, D.C.: Optica Publishing Group, 2006. http://dx.doi.org/10.1364/bosd.1996.ft5.

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Accumulating evidence strongly associates human papillomavirus infection with the development of cervical cancers. However, it has also become increasingly clear that HPV infections of the cervix span a wide clinical spectrum from benign lesions to precancerous lesions, with only a minority of infections resulting in invasive cancers, although the reasons for this are not clear. Longitudinal epidemiologic studies using cytologic methods to detect HPV infection have shown that the majority of women infected with HPV will regress spontaneously. In addition, age-stratified data for rates of HPV positivity from cross-sectional studies also suggest that many women clear the infection spontaneously. These results support the concept that many women may be only transiently infected with HPV during their life span and only in women with persistent HPV infection does cervical cancer progress. In addition to persistence of HPV infection, recent epidemiological studies indicate that the amount of high-risk HPV (viral load or HPV gene copy number) in cervicovaginal epithelial cells may be a risk factor for cervical cancer. Thus, a technique which could detect, genotype and quantitate HPV in smears of cervicovaginal epithelial cells would be of major import in assessment of patient clinical status as well as in epidemiological studies relating HPV infection to cervical cancer.
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Reports on the topic "Cytological techniques"

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Fliedner Theodor M., Feinendegen Ludwig E., Meineke Viktor, and Fritz Thomas E. Responses of Cell Renewal Systems to Long-term Low-Level Radiation Exposure: A Feasibility Study Applying Advanced Molecular Biology Techniques on Available Histological and Cytological Material of Exposed Animals and Men. Office of Scientific and Technical Information (OSTI), February 2005. http://dx.doi.org/10.2172/878157.

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