Journal articles on the topic 'East Texas Lakes'

Natural decrease is no longer rare in the United States. By 1989, 34 percent of all U.S. counties had experienced at least one year of it. Natural decrease is most common in rural areas remote from metropolitan centers. Regional concentrations of natural decrease exist in the Great Plains, the Corn Belt, and East Texas with scattered pockets in the Ozark-Ouachita Uplands, Upper Great Lakes, and Florida. Natural decrease is caused by age structure distortions stimulated by protracted, age-specific migration. Although temporal variations in fertility also contribute to natural decrease, these variations are not due to below average fertility. Natural decrease is symptomatic of fundamental changes in the demographic structure of an area.

An analysis of three predecessor rain events (PREs) that occurred ahead of North Atlantic tropical cyclone (TC) Ike and east Pacific TC Lowell during 10–15 September 2008 is presented. The three PREs produced all-time daily record rainfall at many locations, including Lubbock, Texas (189.5 mm); Wichita, Kansas (262 mm); and Chicago–O’Hare, Illinois (169 mm), on 11–13 September, respectively. PRE 1 organized over Texas on 10 September with moisture from a stalled frontal boundary and the Bay of Campeche, and matured with moisture from TC Lowell. PRE 2 organized over the Texas Panhandle on 11 September with moisture from the Bay of Campeche, and developed and matured over Kansas and Missouri with moisture from TC Lowell. PRE 3 developed over Texas on 11 September, merged with and absorbed PRE 2 over Kansas and Missouri, and matured as it ingested moisture from TC Ike. All three PREs matured in the equatorward entrance region of an intensifying subtropical jet stream (STJ). Heavy rainfall with the three PREs occurred along a plume of moist air characterized by high precipitable water values that extended poleward over the central United States near the juxtaposition of the nose of a low-level jet, a region of lower-tropospheric forcing for ascent along a surface baroclinic zone, and the STJ equatorward entrance region. The cumulative upscale effect of persistent deep convection from the three PREs enhanced and “locked in” a favorable upper-tropospheric flow pattern conducive to ridge development over the Ohio Valley and STJ intensification over the central U.S. and Great Lakes region.

Abstract Cloud-to-ground lightning data from the National Lightning Detection Network are used to create a warm season (May–September) lightning climatology for the northern Gulf of Mexico coast for the 14-yr period 1989–2002. Each day is placed into one of five flow regimes based on the orientation of the low-level flow with respect to the coastline. This determination is made using the vector mean 1000–700-hPa wind data at Lake Charles and Slidell, Louisiana. Flash densities are calculated for daily, hourly, and nocturnal periods. Spatial patterns of composite 24-h and nocturnal flash density indicate that lightning decreases in an east-to-west direction over the region. Flash densities for the 24-h period are greatest over land near the coast, with relative maxima located near Houston, Texas; Lake Charles, Baton Rouge, and New Orleans, Louisiana; Biloxi, Mississippi; and Mobile, Alabama. Flash densities during the nocturnal period are greatest over the coastal waters. Lightning across the northern Gulf coast is closely related to the prevailing low-level synoptic flow, which controls the sea breeze, the dominant forcing mechanism during the warm season. Southwest flow, the most unstable and humid of the five regimes, exhibits the most flashes. In this case, sea-breeze-induced convection is located slightly inland from the coast. Northeast flow, the driest and most stable of the regimes, exhibits the least amount of lightning. The large-scale flow restricts the sea breeze to near the coastline. Geographic features and local mesoscale circulations are found to affect lightning across the region. Geographic features include lakes, bays, marshes, swamps, and coastline orientations. Thermal circulations associated with these features interact with the main sea breeze to produce complex lightning patterns over the area.

Abstract Factor V has recently been observed to act as a synergistic anticoagulant cofactor in the activated Protein C (APC) pathway; however, the importance of factor V’s anticoagulant nature in vivo is poorly understood. A novel autosomal dominant bleeding disorder, which is characterized by excessive bleeding with trauma or surgery and menorrhagia in affected women, was identified in a large family (16 affected individuals) from east Texas. Affected members had a prolongation of their PT and/or aPTT. Clinical studies indicated normal activities and levels of all coagulation factors. Linkage analysis mapped the defective gene to 1q23–24 (LODmax 7.33), which contains the gene for coagulation factor V (FV). An alteration (A2440G)in the FV gene in exon 13, which encodes the entire B-domain of the wildtype protein, segregates with disease and was not present in 62 controls. Interestingly, this alteration falls on the 5′ splice site (TA/GT) of an alternative splicing variant confirmed by RT-PCR of control liver and leukocytes. Furthermore, immunoblot analysis demonstrated that this splice variant produces a low abundance, 250kD isoform of FV in control plasma. The A2440G alteration can theoretically form a higher efficiency consensus site for splicing (TG/GT). Semi-quantitative RT-PCR confirms this improved efficiency with an increase of splicing seen in patients’ RNA from leukocytes (n=3) versus controls (n=3). This translates into an approximately 20-fold increase in the 250kD isoform of FV seen in patients’ plasma (n=6) versus controls (n=5), while wildtype FV levels remain equivalent. Co-precipitation with Vitamin-K dependent coagulation factors from patient plasma suggests that this isoform interacts with known FV-interacting proteins (Figure 1). A recombinant of this splicing event (FV-703) exhibited normal activation by thrombin and normal prothrombinase activity when compared to the wildtype recombinant. Thrombin-activated FV-703 also showed normal degradation by APC, while intact FV-703 displayed an increased sensitivity to APC that was more striking in the presence of PS (Figure 2). We hypothesize that this novel isoform has a stronger affinity for APC in the presence of PS than wildtype FV and works primarily as a cofactor in the APC pathway to heighten the overall anticoagulant activity in the bloodstream. These data indicate that A2440G up-regulates an alternatively spliced transcript of FV, and increases a FV isoform that hinders coagulation as opposed to promoting it like its wildtype counterpart. As verified by this unique mutation, this novel alternative splicing of coagulation FV is an important physiological event that can result in disease if its delicate balance is disrupted. Figure 1: Immunoblot analysis. 8% SDS-PAGE analysis of proteins eluted from aluminum hydroxide Lanes 1 and 7 are protein standard size markers. Lanes 2 and 3 are unrelated controls. Lanes 4–6 are related, unknown disease status patients without the disease haplotype. Lanes 8–10 are related, unknown affected status patients with the disease haplotype. Lanes 11–13 are affected individuals with disease haplotype. FV was detected using monolconal against the heavy chain (AHV-5146), and the Arnerrham ECF Western Blotting Detection Kit was used to develop the western blots. Mr indicates molecular range. Figure 1:. Immunoblot analysis. 8% SDS-PAGE analysis of proteins eluted from aluminum hydroxide Lanes 1 and 7 are protein standard size markers. Lanes 2 and 3 are unrelated controls. Lanes 4–6 are related, unknown disease status patients without the disease haplotype. Lanes 8–10 are related, unknown affected status patients with the disease haplotype. Lanes 11–13 are affected individuals with disease haplotype. FV was detected using monolconal against the heavy chain (AHV-5146), and the Arnerrham ECF Western Blotting Detection Kit was used to develop the western blots. Mr indicates molecular range. Figure 2: Degradation of FV by APC +/− PS. Recombinant wildtype FV and FV-703 were incubated with varying amounts of APC in the absence or presence of 100nM PS for 10 minutes at 37C. After stopping the reaction by dilution, the samples were activated with thrombin and tested in a PTase assay for undegraded FV activity. Figure 2:. Degradation of FV by APC +/− PS. Recombinant wildtype FV and FV-703 were incubated with varying amounts of APC in the absence or presence of 100nM PS for 10 minutes at 37C. After stopping the reaction by dilution, the samples were activated with thrombin and tested in a PTase assay for undegraded FV activity.

The Margaret Hunt Hill Bridge, designed by Spanish architect Santiago Calatrava, is a cable-stayed bridge over the Trinity River in Dallas, Texas. The roadway, almost 1200 ft. long, is supported by fifty-eight cables, twenty-nine on each side of the central arch, strung from the arch to the traffic median between the lanes going (roughly) east or west. The bridge first opened to vehicular traffic in March 2012 and has already been hailed as an icon of twenty-first-century Dallas.

This article examines the strategy of literary spatialization employed by colonial subjects to imaginatively engage with colonial civilizing projects. It analyzes twelve adventure stories written between the 1910s and 1920s by colonial Vietnamese reformed scholars, whose lives were impacted by the pan-Asian reform movements that swept Japan, China, and Vietnam between the 1860s and 1900s. They reflected their experiences with Enlightened civilization as they were pushing for vernacularization and modernization through translating the Chinese transculturation of Japanese texts into Latin-basedquốc ngữscript while constructing a national literature. Adventure tales and travelogues were considered suitable for aspiring writers to translatively imitate Western literature as presented in Chinese translation of Japanese texts. The authors negotiated with the French version of Enlightened Civilization by employing two East Asian literary tropes: the dangerous but exciting Rivers-and-Lakes World, where the protagonist ventures to search forvăn minh, and the peaceful and other-worldly Peach Blossom Spring utopia, where the true qualities ofvăn minhare realized. These stories reveal colonial subjects’ admiration for and anxiety regarding the Frenchmission civilisatrice, and their literary efforts to imagine a Vietnamesevăn minhthat would both impress and surpass the original models.

An annual scientific practical conference “Natural science education in a comprehensive school – 2019” took place in Aukstaitian town Kupiskis. It is known that Kupiskis is a town in the North East of Lithuania, in the eastern edge of Middle Lithuania lowland. The time of the conference is traditional – the second part of April, i.e., from 25th to 27th.. It is worth to remember that a conference “came travelling” from Little Lithuania (Lithuania Minor) to Aukstaitia (Lithuanian Highland) (In 2018, the 24th conference took place in Silutė Martynas Jankus Basic school). Thus, a conference is still “travelling”. On the first conference day, plenary reports and two section works took place. A useful plenary report was made by Eugenija Rudnickaite from Vilnius university. A fossil fuel collection was discussed and presented; some practical tips were given about the application of the collection for natural science education. The second report was made by dr. Agnaldo Arroio. The report was very actual because the actualities of modern media application in education were discussed, also there was a discussion on natural science literacy formation questions. The third report which was made by Vilnius university lecturer dr. Rita Makarskaitė-Petkevičienė, focused on the current natural science questions in primary school. The fourth report, which was made by Latvian university professor Andris Broks, completed the plenary meeting. As always, the professor analysed methodological, systemic natural science education questions. The latter report was focussed on the changes /alteration in nature, society, education and so on. Most of the conference reports were presented in the form of articles and published in the conference proceedings. One can find full texts in the database at: http://oaji.net/journal-archive-stats.html?number=1984&year=2019&issue=14379 Two conference days passed in the blink of an eye. Totally, 66 participants were enrolled in it. The work of the conference went smoothly, therefore it is worthy to thank all the members of the conference organisational committee, and especially the pro-gymnasium administration. Natural science education “journey” continues. The 26th conference will take place on 24th -25th April 2020 in Trakai Vytautas Magnus gymnasium. This gymnasium is the main partner of another conference. So, from Aukstaitia we move to Trakai, a city of Vilnius district, surrounded by lakes. What is more, Trakai is the cultural capital of Lithuania, 2020. Keywords: national conference, science education, science and technological education movement.

Environments containing significant concentration of NaCl such as salt lakes harbor extremophiles microorganisms which have a great biotechnology interest. To explore the diversity of Bacteria in Chott Tinsilt (Algeria), an isolation program was performed. Water samples were collected from the saltern during the pre-salt harvesting phase. This Chott is high in salt (22.47% (w/v). Seven halophiles Bacteria were selected for further characterization. The isolated strains were able to grow optimally in media with 10–25% (w/v) total salts. Molecular identification of the isolates was performed by sequencing the 16S rRNA gene. It showed that these cultured isolates included members belonging to the Halomonas, Staphylococcus, Salinivibrio, Planococcus and Halobacillus genera with less than 98% of similarity with their closest phylogenetic relative. The halophilic bacterial isolates were also characterized for the production of biosurfactant and industrially important enzymes. Most isolates produced hydrolases and biosurfactants at high salt concentration. In fact, this is the first report on bacterial strains (A4 and B4) which were a good biosurfactant and coagulase producer at 20% and 25% ((w/v)) NaCl. In addition, the biosurfactant produced by the strain B4 at high salinity (25%) was also stable at high temperature (30-100°C) and high alkalinity (pH 11).Key word: Salt Lake, Bacteria, biosurfactant, Chott, halophiles, hydrolases, 16S rRNAINTRODUCTIONSaline lakes cover approximately 10% of the Earth’s surface area. The microbial populations of many hypersaline environments have already been studied in different geographical regions such as Great Salt Lake (USA), Dead Sea (Israel), Wadi Natrun Lake (Egypt), Lake Magadi (Kenya), Soda Lake (Antarctica) and Big Soda Lake and Mono Lake (California). Hypersaline regions differ from each other in terms of geographical location, salt concentration and chemical composition, which determine the nature of inhabitant microorganisms (Gupta et al., 2015). Then low taxonomic diversity is common to all these saline environments (Oren et al., 1993). Halophiles are found in nearly all major microbial clades, including prokaryotic (Bacteria and Archaea) and eukaryotic forms (DasSarma and Arora, 2001). They are classified as slight halophiles when they grow optimally at 0.2–0.85 M (2–5%) NaCl, as moderate halophiles when they grow at 0.85–3.4 M (5–20%) NaCl, and as extreme halophiles when they grow at 3.4–5.1 M (20–30%) NaCl. Hyper saline environments are inhabited by extremely halophilic and halotolerant microorganisms such as Halobacillus sp, Halobacterium sp., Haloarcula sp., Salinibacter ruber , Haloferax sp and Bacillus spp. (Solomon and Viswalingam, 2013). There is a tremendous demand for halophilic bacteria due to their biotechnological importance as sources of halophilic enzymes. Enzymes derived from halophiles are endowed with unique structural features and catalytic power to sustain the metabolic and physiological processes under high salt conditions. Some of these enzymes have been reported to be active and stable under more than one extreme condition (Karan and Khare, 2010). Applications are being considered in a range of industries such as food processing, washing, biosynthetic processes and environmental bioremediation. Halophilic proteases are widely used in the detergent and food industries (DasSarma and Arora, 2001). However, esterases and lipases have also been useful in laundry detergents for the removal of oil stains and are widely used as biocatalysts because of their ability to produce pure compounds. Likewise, amylases are used industrially in the first step of the production of high fructose corn syrup (hydrolysis of corn starch). They are also used in the textile industry in the de-sizing process and added to laundry detergents. Furthermore, for the environmental applications, the use of halophiles for bioremediation and biodegradation of various materials from industrial effluents to soil contaminants and accidental spills are being widely explored. In addition to enzymes, halophilic / halotolerants microorganisms living in saline environments, offer another potential applications in various fields of biotechnology like the production of biosurfactant. Biosurfactants are amphiphilic compounds synthesized from plants and microorganisms. They reduce surface tension and interfacial tension between individual molecules at the surface and interface respectively (Akbari et al., 2018). Comparing to the chemical surfactant, biosurfactant are promising alternative molecules due to their low toxicity, high biodegradability, environmental capability, mild production conditions, lower critical micelle concentration, higher selectivity, availability of resources and ability to function in wide ranges of pH, temperature and salinity (Rocha et al., 1992). They are used in various industries which include pharmaceuticals, petroleum, food, detergents, cosmetics, paints, paper products and water treatment (Akbari et al., 2018). The search for biosurfactants in extremophiles is particularly promising since these biomolecules can adapt and be stable in the harsh environments in which they are to be applied in biotechnology.OBJECTIVESEastern Algeria features numerous ecosystems including hypersaline environments, which are an important source of salt for food. The microbial diversity in Chott Tinsilt, a shallow Salt Lake with more than 200g/L salt concentration and a superficies of 2.154 Ha, has never yet been studied. The purpose of this research was to chemically analyse water samples collected from the Chott, isolate novel extremely or moderate halophilic Bacteria, and examine their phenotypic and phylogenetic characteristics with a view to screening for biosurfactants and enzymes of industrial interest.MATERIALS AND METHODSStudy area: The area is at 5 km of the Commune of Souk-Naâmane and 17 km in the South of the town of Aïn-Melila. This area skirts the trunk road 3 serving Constantine and Batna and the railway Constantine-Biskra. It is part the administrative jurisdiction of the Wilaya of Oum El Bouaghi. The Chott belongs to the wetlands of the High Plains of Constantine with a depth varying rather regularly without never exceeding 0.5 meter. Its length extends on 4 km with a width of 2.5 km (figure 1).Water samples and physico-chemical analysis: In February 2013, water samples were collected from various places at the Chott Tinsilt using Global Positioning System (GPS) coordinates of 35°53’14” N lat. and 06°28’44”E long. Samples were collected randomly in sterile polythene bags and transported immediately to the laboratory for isolation of halophilic microorganisms. All samples were treated within 24 h after collection. Temperature, pH and salinity were measured in situ using a multi-parameter probe (Hanna Instruments, Smithfield, RI, USA). The analytical methods used in this study to measure ions concentration (Ca2+, Mg2+, Fe2+, Na+, K+, Cl−, HCO3−, SO42−) were based on 4500-S-2 F standard methods described elsewhere (Association et al., 1920).Isolation of halophilic bacteria from water sample: The media (M1) used in the present study contain (g/L): 2.0 g of KCl, 100.0/200.0 g of NaCl, 1.0 g of MgSO4.7HO2, 3.0 g of Sodium Citrate, 0.36 g of MnCl2, 10.0 g of yeast extract and 15.0 g agar. The pH was adjusted to 8.0. Different dilutions of water samples were added to the above medium and incubated at 30°C during 2–7 days or more depending on growth. Appearance and growth of halophilic bacteria were monitored regularly. The growth was diluted 10 times and plated on complete medium agar (g/L): glucose 10.0; peptone 5.0; yeast extract 5.0; KH2PO4 5.0; agar 30.0; and NaCl 100.0/200.0. Resultant colonies were purified by repeated streaking on complete media agar. The pure cultures were preserved in 20% glycerol vials and stored at −80°C for long-term preservation.Biochemical characterisation of halophilic bacterial isolates: Bacterial isolates were studied for Gram’s reaction, cell morphology and pigmentation. Enzymatic assays (catalase, oxidase, nitrate reductase and urease), and assays for fermentation of lactose and mannitol were done as described by Smibert (1994).Optimization of growth conditions: Temperature, pH, and salt concentration were optimized for the growth of halophilic bacterial isolates. These growth parameters were studied quantitatively by growing the bacterial isolates in M1 medium with shaking at 200 rpm and measuring the cell density at 600 nm after 8 days of incubation. To study the effect of NaCl on the growth, bacterial isolates were inoculated on M1 medium supplemented with different concentration of NaCl: 1%-35% (w/v). The effect of pH on the growth of halophilic bacterial strains was studied by inoculating isolates on above described growth media containing NaCl and adjusted to acidic pH of 5 and 6 by using 1N HCl and alkaline pH of 8, 9, 10, 11 and 12 using 5N NaOH. The effect of temperature was studied by culturing the bacterial isolates in M1 medium at different temperatures of incubation (4°C–55°C).Screening of halophilic bacteria for hydrolytic enzymes: Hydrolase producing bacteria among the isolates were screened by plate assay on starch, tributyrin, gelatin and DNA agar plates respectively for amylase, lipase, protease and DNAse activities. Amylolytic activity of the cultures was screened on starch nutrient agar plates containing g/L: starch 10.0; peptone 5.0; yeast extract 3.0; agar 30.0; NaCl 100.0/250.0. The pH was 7.0. After incubation at 30 ºC for 7 days, the zone of clearance was determined by flooding the plates with iodine solution. The potential amylase producers were selected based on ratio of zone of clearance diameter to colony diameter. Lipase activity of the cultures was screened on tributyrin nutrient agar plates containing 1% (v/v) of tributyrin. Isolates that showed clear zones of tributyrin hydrolysis were identified as lipase producing bacteria. Proteolytic activity of the isolates was similarly screened on gelatin nutrient agar plates containing 10.0 g/L of gelatin. The isolates showing zones of gelatin clearance upon treatment with acidic mercuric chloride were selected and designated as protease producing bacteria. The presence of DNAse activity on plates was determined on DNAse test agar (BBL) containing 10%-25% (w/v) total salt. After incubation for 7days, the plates were flooded with 1N HCl solution. Clear halos around the colonies indicated DNAse activity (Jeffries et al., 1957).Milk clotting activity (coagulase activity) of the isolates was also determined following the procedure described (Berridge, 1952). Skim milk powder was reconstituted in 10 mM aqueous CaCl2 (pH 6.5) to a final concentration of 0.12 kg/L. Enzyme extracts were added at a rate of 0.1 mL per mL of milk. The coagulation point was determined by manual rotating of the test tube periodically, at short time intervals, and checking for visible clot formation.Screening of halophilic bacteria for biosurfactant production. Oil spread Assay: The Petridis base was filled with 50 mL of distilled water. On the water surface, 20μL of diesel and 10μl of culture were added respectively. The culture was introduced at different spots on the diesel, which is coated on the water surface. The occurrence of a clear zone was an indicator of positive result (Morikawa et al., 2000). The diameter of the oil expelling circles was measured by slide caliber (with a degree of accuracy of 0.02 mm).Surface tension and emulsification index (E24): Isolates were cultivated at 30 °C for 7 days on the enrichment medium containing 10-25% NaCl and diesel oil as the sole carbon source. The medium was centrifuged (7000 rpm for 20 min) and the surface tension of the cell-free culture broth was measured with a TS90000 surface tensiometer (Nima, Coventry, England) as a qualitative indicator of biosurfactant production. The culture broth was collected with a Pasteur pipette to remove the non-emulsified hydrocarbons. The emulsifying capacity was evaluated by an emulsification index (E24). The E24 of culture samples was determined by adding 2 mL of diesel oil to the same amount of culture, mixed for 2 min with a vortex, and allowed to stand for 24 h. E24 index is defined as the percentage of height of emulsified layer (mm) divided by the total height of the liquid column (mm).Biosurfactant stability studies : After growth on diesel oil as sole source of carbone, cultures supernatant obtained after centrifugation at 6,000 rpm for 15 min were considered as the source of crude biosurfactant. Its stability was determined by subjecting the culture supernatant to various temperature ranges (30, 40, 50, 60, 70, 80 and 100 °C) for 30 min then cooled to room temperature. Similarly, the effect of different pH (2–11) on the activity of the biosurfactant was tested. The activity of the biosurfactant was investigated by measuring the emulsification index (El-Sersy, 2012).Molecular identification of potential strains. DNA extraction and PCR amplification of 16S rDNA: Total cellular DNA was extracted from strains and purified as described by Sambrook et al. (1989). DNA was purified using Geneclean® Turbo (Q-BIO gene, Carlsbad, CA, USA) before use as a template in polymerase chain reaction (PCR) amplification. For the 16S rDNA gene sequence, the purified DNA was amplified using a universal primer set, forward primer (27f; 5′-AGA GTT TGA TCM TGG CTC AG) and a reverse primer (1492r; 5′-TAC GGY TAC CTT GTT ACG ACT T) (Lane, 1991). Agarose gel electrophoresis confirmed the amplification product as a 1400-bp DNA fragment.16S rDNA sequencing and Phylogenic analysis: Amplicons generated using primer pair 27f-1492r was sequenced using an automatic sequencer system at Macrogene Company (Seoul, Korea). The sequences were compared with those of the NCBI BLAST GenBank nucleotide sequence databases. Phylogenetic trees were constructed by the neighbor-joining method using MEGA version 5.05 software (Tamura et al., 2011). Bootstrap resembling analysis for 1,000 replicates was performed to estimate the confidence of tree topologies.Nucleotide sequence accession numbers: The nucleotide sequences reported in this work have been deposited in the EMBL Nucleotide Sequence Database. The accession numbers are represented in table 5.Statistics: All experiments were conducted in triplicates. Results were evaluated for statistical significance using ANOVA.RESULTSPhysico-chemical parameters of the collected water samples: The physicochemical properties of the collected water samples are reported in table 1. At the time of sampling, the temperature was 10.6°C and pH 7.89. The salinity of the sample, as determined in situ, was 224.70 g/L (22,47% (w/v)). Chemical analysis of water sample indicated that Na +and Cl- were the most abundant ions (table 1). SO4-2 and Mg+2 was present in much smaller amounts compared to Na +and Cl- concentration. Low levels of calcium, potassium and bicarbonate were also detected, often at less than 1 g/L.Characterization of isolates. Morphological and biochemical characteristic feature of halophilic bacterial isolates: Among 52 strains isolated from water of Chott Tinsilt, seven distinct bacteria (A1, A2, A3, A4, B1, B4 and B5) were chosen for further characterization (table 2). The colour of the isolates varied from beige, pale yellow, yellowish and orange. The bacterial isolates A1, A2, A4, B1 and B5 were rod shaped and gram negative (except B5), whereas A3 and B4 were cocci and gram positive. All strains were oxidase and catalase positive except for B1. Nitrate reductase and urease activities were observed in all the bacterial isolates, except B4. All the bacterial isolates were negative for H2S formation. B5 was the only strain positive for mannitol fermentation (table 2).We isolated halophilic bacteria on growth medium with NaCl supplementation at pH 7 and temperature of 30°C. We studied the effect of NaCl, temperature and pH on the growth of bacterial isolates. All the isolates exhibited growth only in the presence of NaCl indicating that these strains are halophilic. The optimum growth of isolates A3 and B1 was observed in the presence of 10% NaCl, whereas it was 15% NaCl for A1, A2 and B5. A4 and B4 showed optimum growth in the presence of 20% and 25% NaCl respectively. A4, B4 and B5 strains can tolerate up to 35% NaCl.The isolate B1 showed growth in medium supplemented with 10% NaCl and pH range of 7–10. The optimum pH for the growth B1 was 9 and they did not show any detectable growth at or below pH 6 (table 2), which indicates the alkaliphilic nature of B1 isolate. The bacterial isolates A1, A2 and A4 exhibited growth in the range of pH 6–10, while A3 and B4 did not show any growth at pH greater than 8. The optimum pH for growth of all strains (except B1) was pH 7.0 (table 2). These results indicate that A1, A2, A3, A4, B4 and B5 are neutrophilic in nature. All the bacterial isolates exhibited optimal growth at 30°C and no detectable growth at 55°C. Also, detectable growth of isolates A1, A2 and A4 was observed at 4°C. However, none of the bacterial strains could grow below 4°C and above 50°C (table 2).Screening of the halophilic enzymes: To characterize the diversity of halophiles able to produce hydrolytic enzymes among the population of microorganisms inhabiting the hypersaline habitats of East Algeria (Chott Tinsilt), a screening was performed. As described in Materials and Methods, samples were plated on solid media containing 10%-25% (w/v) of total salts and different substrates for the detection of amylase, protease, lipase and DNAse activities. However, coagulase activity was determined in liquid medium using milk as substrate (figure 3). Distributions of hydrolytic activity among the isolates are summarized in table 4.From the seven bacterial isolates, four strains A1, A2, A4 and B5 showed combined hydrolytic activities. They were positive for gelatinase, lipase and coagulase. A3 strain showed gelatinase and lipase activities. DNAse activities were detected with A1, A4, B1 and B5 isolates. B4 presented lipase and coagulase activity. Surprisingly, no amylase activity was detected among all the isolates.Screening for biosurfactant producing isolates: Oil spread assay: The results showed that all the strains could produce notable (>4 cm diameter) oil expelling circles (ranging from 4.11 cm to 4.67 cm). The average diameter for strain B5 was 4.67 cm, significantly (P < 0.05) higher than for the other strains.Surface tension and emulsification index (E24): The assimilation of hydrocarbons as the sole sources of carbon by the isolate strains led to the production of biosurfactants indicated by the emulsification index and the lowering of the surface tension of cell-free supernatant. Based on rapid growth on media containing diesel oil as sole carbon source, the seven isolates were tested for biosurfactant production and emulsification activity. The obtained values of the surface tension measurements as well as the emulsification index (E24) are shown in table 3. The highest reduction of surface tension was achieved with B5 and A3 isolates with values of 25.3 mN m−1 and 28.1 mN m−1 respectively. The emulsifying capacity evaluated by the E24 emulsification index was highest in the culture of isolate B4 (78%), B5 (77%) and A3 (76%) as shown in table 3 and figure 2. These emulsions were stable even after 4 months. The bacteria with emulsification indices higher than 50 % and/or reduction in the surface tension (under 30 mN/m) have been defined as potential biosurfactant producers. Based on surface tension and the E24 index results, isolates B5, B4, A3 and A4 are the best candidates for biosurfactant production. It is important to note that, strains B4 and A4 produce biosurfactant in medium containing respectively 25% and 20% (w/v) NaCl.Stability of biosurfactant activities: The applicability of biosurfactants in several biotechnological fields depends on their stability at different environmental conditions (temperatures, pH and NaCl). For this study, the strain B4 appear very interesting (It can produce biosurfactant at 25 % NaCl) and was choosen for futher analysis for biosurfactant stability. The effects of temperature and pH on the biosurfactant production by the strain B4 are shown in figure 4.biosurfactant in medium containing respectively 25% and 20% (w/v) NaCl.Stability of biosurfactant activities: The applicability of biosurfactants in several biotechnological fields depends on their stability at different environmental conditions (temperatures, pH and NaCl). For this study, the strain B4 appear very interesting (It can produce biosurfactant at 25 % NaCl) and was chosen for further analysis for biosurfactant stability. The effects of temperature and pH on the biosurfactant production by the strain B4 are shown in figure 4. The biosurfactant produced by this strain was shown to be thermostable giving an E-24 Index value greater than 78% (figure 4A). Heating of the biosurfactant to 100 °C caused no significant effect on the biosurfactant performance. Therefore, the surface activity of the crude biosurfactant supernatant remained relatively stable to pH changes between pH 6 and 11. At pH 11, the value of E24 showed almost 76% activity, whereas below pH 6 the activity was decreased up to 40% (figure 4A). The decreases of the emulsification activity by decreasing the pH value from basic to an acidic region; may be due to partial precipitation of the biosurfactant. This result indicated that biosurfactant produced by strain B4 show higher stability at alkaline than in acidic conditions.Molecular identification and phylogenies of potential isolates: To identify halophilic bacterial isolates, the 16S rDNA gene was amplified using gene-specific primers. A PCR product of ≈ 1.3 kb was detected in all the seven isolates. The 16S rDNA amplicons of each bacterial isolate was sequenced on both strands using 27F and 1492R primers. The complete nucleotide sequence of 1336,1374, 1377,1313, 1305,1308 and 1273 bp sequences were obtained from A1, A2, A3, A4, B1, B4 and B5 isolates respectively, and subjected to BLAST analysis. The 16S rDNA sequence analysis showed that the isolated strains belong to the genera Halomonas, Staphylococcus, Salinivibrio, Planococcus and Halobacillus as shown in table 5. The halophilic isolates A2 and A4 showed 97% similarity with the Halomonas variabilis strain GSP3 (accession no. AY505527) and the Halomonas sp. M59 (accession no. AM229319), respectively. As for A1, it showed 96% similarity with the Halomonas venusta strain GSP24 (accession no. AY553074). B1 and B4 showed for their part 96% similarity with the Salinivibrio costicola subsp. alcaliphilus strain 18AG DSM4743 (accession no. NR_042255) and the Planococcus citreus (accession no. JX122551), respectively. The bacterial isolate B5 showed 98% sequence similarity with the Halobacillus trueperi (accession no. HG931926), As for A3, it showed only 95% similarity with the Staphylococcus arlettae (accession no. KR047785). The 16S rDNA nucleotide sequences of all the seven halophilic bacterial strains have been submitted to the NCBI GenBank database under the accession number presented in table 5. The phylogenetic association of the isolates is shown in figure 5.DICUSSIONThe physicochemical properties of the collected water samples indicated that this water was relatively neutral (pH 7.89) similar to the Dead Sea and the Great Salt Lake (USA) and in contrast to the more basic lakes such as Lake Wadi Natrun (Egypt) (pH 11) and El Golea Salt Lake (Algeria) (pH 9). The salinity of the sample was 224.70 g/L (22,47% (w/v). This range of salinity (20-30%) for Chott Tinsilt is comparable to a number of well characterized hypersaline ecosystems including both natural and man-made habitats, such as the Great Salt Lake (USA) and solar salterns of Puerto Rico. Thus, Chott Tinsilt is a hypersaline environment, i.e. environments with salt concentrations well above that of seawater. Chemical analysis of water sample indicated that Na +and Cl- were the most abundant ions, as in most hypersaline ecosystems (with some exceptions such as the Dead Sea). These chemical water characteristics were consistent with the previously reported data in other hypersaline ecosystems (DasSarma and Arora, 2001; Oren, 2002; Hacěne et al., 2004). Among 52 strains isolated from this Chott, seven distinct bacteria (A1, A2, A3, A4, B1, B4 and B5) were chosen for phenotypique, genotypique and phylogenetique characterization.The 16S rDNA sequence analysis showed that the isolated strains belong to the genera Halomonas, Staphylococcus, Salinivibrio, Planococcus and Halobacillus. Genera obtained in the present study are commonly occurring in various saline habitats across the globe. Staphylococci have the ability to grow in a wide range of salt concentrations (Graham and Wilkinson, 1992; Morikawa et al., 2009; Roohi et al., 2014). For example, in Pakistan, Staphylococcus strains were isolated from various salt samples during the study conducted by Roohi et al. (2014) and these results agreed with previous reports. Halomonas, halophilic and/or halotolerant Gram-negative bacteria are typically found in saline environments (Kim et al., 2013). The presence of Planococcus and Halobacillus has been reported in studies about hypersaline lakes; like La Sal del Rey (USA) (Phillips et al., 2012) and Great Salt Lake (Spring et al., 1996), respectively. The Salinivibrio costicola was a representative model for studies on osmoregulatory and other physiological mechanisms of moderately halophilic bacteria (Oren, 2006).However, it is interesting to note that all strains shared less than 98.7% identity (the usual species cut-off proposed by Yarza et al. (2014) with their closest phylogenetic relative, suggesting that they could be considered as new species. Phenotypic, genetic and phylogenetic analyses have been suggested for the complete identification of these strains. Theses bacterial strains were tested for the production of industrially important enzymes (Amylase, protease, lipase, DNAse and coagulase). These isolates are good candidates as sources of novel enzymes with biotechnological potential as they can be used in different industrial processes at high salt concentration (up to 25% NaCl for B4). Prominent amylase, lipase, protease and DNAase activities have been reported from different hypersaline environments across the globe; e.g., Spain (Sánchez‐Porro et al., 2003), Iran (Rohban et al., 2009), Tunisia (Baati et al., 2010) and India (Gupta et al., 2016). However, to the best of our knowledge, the coagulase activity has never been detected in extreme halophilic bacteria. Isolation and characterization of crude enzymes (especially coagulase) to investigate their properties and stability are in progress.The finding of novel enzymes with optimal activities at various ranges of salt concentrations is of great importance. Besides being intrinsically stable and active at high salt concentrations, halophilic and halotolerant enzymes offer great opportunities in biotechnological applications, such as environmental bioremediation (marine, oilfiel) and food processing. The bacterial isolates were also characterized for production of biosurfactants by oil-spread assay, measurement of surface tension and emulsification index (E24). There are few reports on biosurfactant producers in hypersaline environments and in recent years, there has been a greater increase in interest and importance in halophilic bacteria for biomolecules (Donio et al., 2013; Sarafin et al., 2014). Halophiles, which have a unique lipid composition, may have an important role to play as surface-active agents. The archae bacterial ether-linked phytanyl membrane lipid of the extremely halophilic bacteria has been shown to have surfactant properties (Post and Collins, 1982). Yakimov et al. (1995) reported the production of biosurfactant by a halotolerant Bacillus licheniformis strain BAS 50 which was able to produce a lipopeptide surfactant when cultured at salinities up to 13% NaCl. From solar salt, Halomonas sp. BS4 and Kocuria marina BS-15 were found to be able to produce biosurfactant when cultured at salinities of 8% and 10% NaCl respectively (Donio et al., 2013; Sarafin et al., 2014). In the present work, strains B4 and A4 produce biosurfactant in medium containing respectively 25% and 20% NaCl. To our knowledge, this is the first report on biosurfactant production by bacteria under such salt concentration. Biosurfactants have a wide variety of industrial and environmental applications (Akbari et al., 2018) but their applicability depends on their stability at different environmental conditions. The strain B4 which can produce biosurfactant at 25% NaCl showed good stability in alkaline pH and at a temperature range of 30°C-100°C. Due to the enormous utilization of biosurfactant in detergent manufacture the choice of alkaline biosurfactant is researched (Elazzazy et al., 2015). On the other hand, the interesting finding was the thermostability of the produced biosurfactant even after heat treatment (100°C for 30 min) which suggests the use of this biosurfactant in industries where heating is of a paramount importance (Khopade et al., 2012). To date, more attention has been focused on biosurfactant producing bacteria under extreme conditions for industrial and commercial usefulness. In fact, the biosurfactant produce by strain B4 have promising usefulness in pharmaceutical, cosmetics and food industries and for bioremediation in marine environment and Microbial enhanced oil recovery (MEOR) where the salinity, temperature and pH are high.CONCLUSIONThis is the first study on the culturable halophilic bacteria community inhabiting Chott Tinsilt in Eastern Algeria. Different genera of halotolerant bacteria with different phylogeneticaly characteristics have been isolated from this Chott. Culturing of bacteria and their molecular analysis provides an opportunity to have a wide range of cultured microorganisms from extreme habitats like hypersaline environments. Enzymes produced by halophilic bacteria show interesting properties like their ability to remain functional in extreme conditions, such as high temperatures, wide range of pH, and high salt concentrations. These enzymes have great economical potential in industrial, agricultural, chemical, pharmaceutical, and biotechnological applications. Thus, the halophiles isolated from Chott Tinsilt offer an important potential for application in microbial and enzyme biotechnology. In addition, these halo bacterial biosurfactants producers isolated from this Chott will help to develop more valuable eco-friendly products to the pharmacological and food industries and will be usefulness for bioremediation in marine environment and petroleum industry.ACKNOWLEDGMENTSOur thanks to Professor Abdelhamid Zoubir for proofreading the English composition of the present paper.CONFLICT OF INTERESTThe authors declare that they have no conflict of interest.Akbari, S., N. H. Abdurahman, R. M. Yunus, F. Fayaz and O. R. Alara, 2018. Biosurfactants—a new frontier for social and environmental safety: A mini review. Biotechnology research innovation, 2(1): 81-90.Association, A. P. H., A. W. W. Association, W. P. C. Federation and W. E. Federation, 1920. Standard methods for the examination of water and wastewater. American Public Health Association.Baati, H., R. Amdouni, N. Gharsallah, A. Sghir and E. Ammar, 2010. Isolation and characterization of moderately halophilic bacteria from tunisian solar saltern. Current microbiology, 60(3): 157-161.Berridge, N., 1952. Some observations on the determination of the activity of rennet. Analyst, 77(911): 57b-62.DasSarma, S. and P. Arora, 2001. Halophiles. Encyclopedia of life sciences. Nature publishishing group: 1-9.Donio, M. B. S., F. A. Ronica, V. T. Viji, S. Velmurugan, J. S. C. A. Jenifer, M. Michaelbabu, P. Dhar and T. Citarasu, 2013. Halomonas sp. Bs4, a biosurfactant producing halophilic bacterium isolated from solar salt works in India and their biomedical importance. SpringerPlus, 2(1): 149.El-Sersy, N. A., 2012. Plackett-burman design to optimize biosurfactant production by marine Bacillus subtilis n10. Roman biotechnol lett, 17(2): 7049-7064.Elazzazy, A. M., T. Abdelmoneim and O. Almaghrabi, 2015. Isolation and characterization of biosurfactant production under extreme environmental conditions by alkali-halo-thermophilic bacteria from Saudi Arabia. Saudi journal of biological Sciences, 22(4): 466-475.Graham, J. E. and B. Wilkinson, 1992. Staphylococcus aureus osmoregulation: Roles for choline, glycine betaine, proline, and taurine. Journal of bacteriology, 174(8): 2711-2716.Gupta, S., P. Sharma, K. Dev and A. Sourirajan, 2016. Halophilic bacteria of lunsu produce an array of industrially important enzymes with salt tolerant activity. Biochemistry research international, 1: 1-10.Gupta, S., P. Sharma, K. Dev, M. Srivastava and A. Sourirajan, 2015. A diverse group of halophilic bacteria exist in lunsu, a natural salt water body of Himachal Pradesh, India. SpringerPlus 4(1): 274.Hacěne, H., F. Rafa, N. Chebhouni, S. Boutaiba, T. Bhatnagar, J. C. Baratti and B. Ollivier, 2004. Biodiversity of prokaryotic microflora in el golea salt lake, Algerian Sahara. Journal of arid environments, 58(3): 273-284.Jeffries, C. D., D. F. Holtman and D. G. Guse, 1957. Rapid method for determining the activity of microorgan-isms on nucleic acids. Journal of bacteriology, 73(4): 590.Karan, R. and S. Khare, 2010. Purification and characterization of a solvent‐stable protease from Geomicrobium sp. Emb2. Environmental technology, 31(10): 1061-1072.Khopade, A., R. Biao, X. Liu, K. Mahadik, L. Zhang and C. Kokare, 2012. Production and stability studies of the biosurfactant isolated from marine Nocardiopsis sp. B4. Desalination, 3: 198-204.Kim, K. K., J.-S. Lee and D. A. Stevens, 2013. Microbiology and epidemiology of Halomonas species. Future microbiology, 8(12): 1559-1573.Lane, D., 1991. 16s/23s rRNA sequencing in nucleic acid techniques in bacterial systematics. Stackebrandt e., editor;, and goodfellow m., editor. Chichester, UK: John Wiley & Sons.Morikawa, K., R. L. Ohniwa, T. 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Schleifer, W. B. Whitman, J. Euzéby, R. Amann and R. Rosselló-Móra, 2014. Uniting the classification of cultured and uncultured bacteria and archaea using 16s rRNA gene sequences. Nature reviews microbiology, 12(9): 635-645

Due to recent droughty conditions in East Texas in 2010 and 2011, the water levels on the man-made lakes and reservoirs in the region have been steadily lowering. This decreasing water levels is exposing considerable areas along the lakes that not only have been underwater for considerable periods of time since the lakes were constructed, but this new land exposure is also exposing and eroding archaeological sites that are now along the new lake shore boundaries. Such is the case at Lake Jacksonville, a small lake on Gum Creek in Cherokee County, Texas, and newly recorded archaeological sites have been found along its shores. This article is a summary of efforts to document archaeological collections that have been reported from five sites at Lake Jacksonville.

Toledo Bend Reservoir is one of the largest artificial lakes in the United States and the largest reservoir in the South. The lake is approximately 65 miles long and contains over 1200 miles of shoreline in both Louisiana and Texas. Construction began in 1964 with completion of the power plant, with the subsequent filling of the lake in 1969. Archaeological investigations at Toledo Bend Reservoir on the Sabine River and tributaries in both Louisiana and Texas took place primarily took during the 1960s, with survey and excavations, sometimes of a very limited nature by the University of Texas (UT) and Southern Methodist University (SMU). Girard has continued archaeological investigations along the Louisiana side of the reservoir, however, focusing particularly on work at the James Pace site. In this article we review the nature of the material culture assemblage of the Woodland and Caddo sites at Toledo Bend Reservoir based on the collections at the Texas Archeological Research Laboratory at The University of Texas at Austin (TARL). This consists of ceramic and/or lithic artifacts from 76 different sites in Louisiana and Texas. We have also examined ceramic vessels from Woodland and Caddo burial features at several Toledo Bend Reservoir sites. Our purpose in re-examining the TARL collections from the Toledo Bend Reservoir is to better understand and characterize the material culture assemblages (primarily decorated ceramic sherds) from sites that date between ca. 2500 years B.P. and the late 17th-early 18th century A.D., particularly in light of questions concerning the cultural affiliation and cultural taxonomic relationships of the ancestral Caddo sites in this part of East Texas and western Louisiana.

During the late 1700s, the Kadohadacho (hereafter Caddo), a peaceful tribe of agriculturists and hunters, weakened by near-constant pressure from the more war-like Osage and the ravages of various epidemics, began to migrate from their traditional homeland near the Great Bend of the Red River south into northwestern Louisiana and adjacent East Texas. By the time of the Louisiana Purchase in 1803, the Caddo villages under their caddi Dehahuit were concentrated in the Sodo Lakes region west and northwest of modern-day Shreveport. Much of what we know today about the location of these settlements, and specifically Dehahuit’s village, are found in the writings of Indian agents and the field notes and plat maps of period surveyors. In this article we will examine these and other relevant documents and present a case for the location of the village of this historic figure. It is the contention of the author, based on material to be presented in the pages to follow, that Dehahuit’s village will ultimately be found to lie northwest of Waskom, Texas on the southern terraces of Paw Paw Bayou beneath the neatly manicured lawns of the Victoria Wood subdivision. The site is depicted on the 1838 American surveys of northwestern Louisiana as lying on the Natchitoches-to-Pecan Point Road on the section line between Sections 2 and 3 of T17N, R17W. In support of this argument, we will examine in this article (1) a relatively definitive 1840 statement regarding the location of Dehahuit’s village by Jehiel Brooks, long-time Caddo Agent and the individual who engineered the sale of the Caddo lands to the United States; (2) the well-known 1805 statement of Red River Agent John Sibley in which he noted that the Caddo lived some 35 miles west of the Red River on a bayou “called, by them, Sodo;” and (3) a memorial statement by the Caddo themselves regarding the re-location of some of their villages as required by Article 4 of the 1835 treaty cession.

The Boxed Springs Mound site (41UR30) is one of three major Early Caddoan (ca. A.D. 900- t 200) multiple mound centers in the Sabine River basin of northeastern Texas, the others including the Jamestown (41SM54) and Hudnall-Pirtle (41RK4) sites upstream and downstream, respectively, from Boxed Springs. It is situated on a large and prominent upland ridge projection that extends from a bluff on the Sabine River about 500 m north to where the landform merges with a broader stretch of uplands and Bienville alluvium. Sediments on the site are Trep loamy fine sand, a relatively fertile soil. The site is approximately 1.6 km west of the confluence of Big Sandy Creek and the Sabine River, but the old channels, sloughs, and oxbow lakes on both sides of the upland ridge and alluvial terrace suggest that previous channels of the Sabine River as well as Big Sandy Creek ran from north to south immediately adjacent to the site. When the Boxed Springs site was originally recorded by Sam Whiteside, an avocational archeologist from Tyler (see Walters and Haskins, this volume) in the early 1960s, it had four earthen mounds arranged around an open area or central plaza. The four mounds apparently included two low "structural" or house mounds with clay floors at the southeastern and southwestern ends of the plaza (Mounds #2 and #7 on a ca. 1962 sketch by Whiteside), one burial mound about 12 x 8 m in size and 1 m in height at the northwestern plaza edge (Mound #3), and a flat-topped mound of unknown function at the northeastern end of the plaza (Mound #6). There were borrow pits apparently visible to the east of Mound #3 and south of Mound #6, and occupation areas/midden deposits along the uplands at the southern edge of the site as well as north and northwest of Mound #3. Some years ago, while Dr. James E. Bruseth and Dr. Timothy K. Perttula were documenting a large collection of vessels and stone tools from the Boxed Springs site, they became aware of the fact that a cremation burial with associated vessels had been dug at the site. A few years later, the cremated remains from that burial were turned over to Dr. Perttula for study. In this paper, Diane E. Wilson summarizes for the first time the results of her bioarchaeological analyses of the cremated burial. With this information now available, it seemed appropriate to provide an archaeological context--as it was known--on the cremated burial, and also summarize in one place the available information on the archaeological record from the Boxed Springs site. Key to this effort was the fact that Mark Walters provided unpublished information and notes from the 1960s archaeological investigations by Sam Whiteside at the Boxed Springs site. Although it is a major Early Caddoan mound center, the archaeology of the Boxed Springs site is very poorly known. We hope that this paper on a cremated burial from the site, as well as a discussion of previous archaeological investigations at Boxed Springs, will rectify this situation to a certain extent, and also spur renewed professional archaeological interest in this very significant prehistoric Caddoan mound center.

Site 41SM91 is an ancestral Caddo habitation site on an upland landform east of the Neches River, in the area of Lake Palestine, a large reservoir constructed on the Neches River in the East Texas Pineywoods; the dam is located about 11 km south of the site. The site was found and recorded during a 1957 survey of the proposed reservoir flood pool, and Johnson described it as “a large Frankston Focus habitation site located in a cultivated field on the slope of a large hill to the east of the Neches floodplain”. A large assemblage of ceramic vessel sherds were collected from the surface of the site during the archaeological survey, and these sherds are in the collections of the Texas Archeological Research Laboratory at the University of Texas at Austin.

I fell heir to a collection of prehistoric Caddo sherds from my late Uncle Sam Whiteside and a location on a 7.5’ topographic map labeled Lake Clear. Sam Whiteside during the period from the mid-1950s to the late 1960s was very active in the East Texas area working through the old East Texas Archeological Society headquartered in Tyler, Texas. The name Lake Clear was vaguely familiar but a check of local maps failed to reveal its location. However, an older map jolted my memory. The lake had been built on east Mill Creek west of Winona, Texas, as a club lake. For some reason, the project was abandoned after a short period of time and the lake was drained and turned into pasture. Although I have never even visited the site location, but did record it in 2000, I felt that writing this article was justified in order to (1) describe this interesting collection of Caddo sherds and (2) compare one aspect of the sherds—the engraved rattlesnake motif found on several Lake Clear sherds—to other known examples in the Caddo archeological area. This was not a new idea but had been hashed around in prior years by Tim Perttula, Tom Middlebrook, and the late Jim Corbin, among others. Tim Perttula was especially helpful in sharing his notes. Perhaps the most important part of research is knowing when to stop. I realize that other examples of the rattlesnake motif in Caddo pottery will appear in the future, hopefully spurred on by this article, and if someone else would like to take up the subject where I have left off, I would be pleased.

Horizon Environmental Services, Inc. (Horizon) was selected by LJA Engineering, Inc. (LJA), on behalf of Montgomery County Municipal Utility District (MUD) No. 157, to conduct a cultural resources inventory and assessment for the proposed West Fork of the San Jacinto River South Drainage Channel Phase 3 Project. The proposed undertaking would consist of constructing a new outfall structure within an approximately 1.5-hectare (3.6-acre) area on the eastern bank of the West Fork of the San Jacinto River near Moorhead Road. The project area is located on a broad coastal flat that has been historically mined for borrow materials, and numerous artificial lakes that represent infilled borrow pits characterize the surrounding area. The project area is bounded on the southeast by the channel of the West Fork of the San Jacinto River and on the northeast by a large pond that represents an infilled borrow pit. A prominent earthen levee parallels the river channel, and a massive erosional gully runs through the southern portion of the project area. The Liberty Materials, Inc. Moorehead Wet Plant is located approximately 0.8 kilometer (0.5 mile) east of the project area. For purposes of the cultural resources survey, the project area is considered to consist of the entire 1.5-hectare (3.6-acre) outfall construction area. The proposed undertaking would be sponsored by Montgomery County Municipal Utility District (MUD) No. 157, which represents a political subdivision of the state of Texas. As such, the project falls under the jurisdiction of the Antiquities Code of Texas. In addition, the project may require the use of Nationwide Permits (NWP) issued by the US Army Corps of Engineers (USACE), Galveston District, for construction within or adjacent to any water features that meet the criteria for designation as “waters of the US” under Section 404 of the Clean Water Act and/or Section 10 of the Rivers and Harbors Act. As NWPs are federal permits, those portions of the overall project area located within the federal permit area would fall under the jurisdiction of Section 106 of the National Historic Preservation Act (NHPA) of 1966, as amended. As the proposed project represents a publicly sponsored undertaking, the project sponsor is required to provide the applicable federal agencies and the Texas Historical Commission (THC), which serves as the State Historic Preservation Office (SHPO) for the state of Texas, with an opportunity to review and comment on the project’s potential to adversely affect historic properties listed on or considered eligible for listing on the National Register of Historic Places (NRHP) and/or for designation as State Antiquities Landmarks (SAL). On March 28, 2020, Horizon archeologists Jesse Dalton and Jared Wiersema under the overall direction of Jeffrey D. Owens, Principal Investigator, performed an intensive cultural resources survey of the project area to locate any cultural resources that potentially would be impacted by the proposed undertaking. The survey was performed under Texas Antiquities Permit No. 9315. Horizon’s archeologists traversed the archeological survey area on foot and thoroughly inspected the modern ground surface for aboriginal and historic-age cultural resources. The survey area consisted of a flat, heavily modified landform situated between the West Fork of the San Jacinto River channel to the southwest and an artificial lake that represents an infilled borrow pit to the northeast. Historical aerial photographs suggest that past borrow material mining activities have extensively impacted the landform. An earthen levee runs along the edge of the landform parallel to the river channel, and a massive erosional gully passes through the southern portion of the project area. Vegetation within the project area consists primarily of short grasses, and artificial pea gravel deposits cover large portions of the modern ground surface. Overall, ground surface visibility was poor to fair (20 to 40%), though the landform that characterizes the project area appears to have been heavily disturbed and may be almost entirely man-made. In addition to pedestrian walkover, the Texas State Minimum Archeological Survey Standards (TSMASS) require a minimum of two shovel tests per 0.4 hectare (1.0 acres) for tracts between 1.2 and 4.0 hectares (3.0 and 10.0 acres) in size. As such, a minimum of seven shovel tests would be required within the 1.5-hectare (3.6-acre) project area. However, given the physiographic setting of the project area on the floodplain of the West Fork of the San Jacinto River, the extent of prior disturbances associated with historical borrow material mining, and the geomorphological potential for deeply buried Holocene-age sediments, Horizon determined that shovel testing would not likely be a productive survey technique. As such, mechanical trenching was utilized instead. Horizon excavated seven trackhoe trenches within the floodplain of the West Fork of the San Jacinto River in the northern portion of the project area. Trenches ranged from 5.0 to 7.0 meters (16.4 to 23.0 feet) in length and from to 1.5 to 5.5 meters (4.9 to 18.0 feet) in depth. Most trenches were terminated at depths of approximately 2.0 meters (6.6 feet) below surface, though two trenches were excavated to depths of 3.0 to 5.5 meters (9.8 to 18.0 feet) below surface where possible to investigate the potential for more deeply buried natural soil horizons and/or archeological deposits. Sediments observed in all trenches consisted of lenses of artificial fill materials resulting from borrow material mining activities over the years. These sediments tended to be poorly structured and unconsolidated, resulting in frequent collapses of trench walls. The water table was encountered in one trench at a depth of 1.8 meters below surface. While it is possible that some of the more deeply buried clay layers are of natural origin, the vast majority of the sediments observed were clearly artificial fill deposits. No cultural resources, prehistoric or historic-age, were observed on the modern ground surface or within any of the shovel tests excavated within the project area. As no cultural resources were observed during the survey, no cultural resources were collected. Following completion of the project, all project records will be prepared for permanent curation at the Texas Archeological Research Laboratory (TARL). Based on the results of the survey-level investigations documented in this report, no potentially significant cultural resources would be affected by the proposed undertaking. In accordance with 36 CFR 800.4, Horizon has made a reasonable and good-faith effort to identify historic properties within the project area. No cultural resources were identified within the project area that meet the criteria for designation as SALs according to 13 TAC 26 or for inclusion in the NRHP under 36 CFR 60.4. Horizon recommends a finding of “no historic properties affected,” and no further archeological work is recommended in connection with the proposed undertaking. However, human burials, both prehistoric and historic, are protected under the Texas Health and Safety Code. In the event that any human remains or burial objects are inadvertently discovered at any point during construction, use, or ongoing maintenance in the project area, even in previously surveyed areas, all work should cease immediately in the vicinity of the inadvertent discovery, and the Texas Historical Commission (THC) should be notified immediately.

Ceramic pipes are an important part of the ancestral Caddo material culture in all parts of the Caddo area from as early as ca. A.D. 800, and there are also ceramic pipes known from Woodland period sites in the Caddo area. The Caddo pipe forms known include long–stemmed (up to 61 cm in length) Red River pipes, elbow pipes of several varieties, and platform pipes. All three pipe forms are known from Caddo sites at Lake Sam Rayburn in the Angelina River basin in East Texas.

The Stover Lake site (41BW8) is an ancestral Caddo cemetery and habitation site on a natural alluvial rise in the Sulphur River floodplain, about 1.6 km east of the Lake Wright Patman dam. In 1961-1962, several collectors excavated at least 19 Caddo burials at the site and also gathered a collection of sherds from habitation contexts. Notes on the burials and their funerary offerings were provided by the collectors to the Texas Archeological Research Laboratory at The University of Texas at Austin (TARL), and 390 ceramic sherds and one stone gorget from non-burial contexts were donated to TARL by one of the collectors, Janson L. McVay.

This is the third in a series of publications that concern the documentation of prehistoric artifact-collections from sites found along the shoreline of Lake Bob Sandlin in the Big Cypress Creek basin of East Texas. These documentation efforts have demonstrated that sites at the lake have diverse temporal and spatial patterns, with an intensive Caddo occupation from the Middle (ca. A.D. 1200-1425) to Late Caddo (ca. A.D. 1430-1680) periods.

The Pearson site (41RA5) in the Blackland Prairie of East Texas is one of a number of aboriginal archaeological sites recorded during a 1957 archaeological survey of the flood pool of then proposed Lake Tawakoni on the Sabine River; the site is now inundated. The Pearson site was located on several low sandy rises across ca. 25 acres in the Hooker Creek-Sabine River floodplain, and these rises had both aboriginal and European artifacts on the surface. Johnson and Jelks, and Duffield and Jelks have argued that the Pearson site was the Tawakoni-Yscani village visited by a Spanish missionary in 1760 and part of a recently defined Norteno focus, a complex of sites apparently associated with the Wichita tribes. Schambach, by contrast, considers the Pearson site to be an 18th century Tunican entrepot.

Ancestral Caddo habitation sites are common in the upper Sabine River basin in East Texas, as well as along tributaries of the Sabine River, including Lake Fork Creek. In this article we discuss the ceramic vessel sherd assemblages from the Spoonbill site (41WD109) that was investigated in the area in the 1970s. The site is in the Lake Fork Creek basin in the immediate vicinity of Lake Fork Reservoir.

From October 2000 to February 2001, personnel from Prewitt and Associates, Inc., conducted National Register of Historic Places eligibility testing of nine sites located at Waco Lake in McLennan County, Texas. The U.S. Army Corps of Engineers, Fort Worth District, sponsored the project as a result of the proposed plan to raise the conservation pool level of the lake by 7 ft to 462 ft above mean sea level. Waco Lake is located in central McLennan County, Texas, on the west side of the City of Waco (Figure 1). It is on the Bosque River, with the dam lying ca. 6.6 km upstream from where the Bosque flows into the Brazos River. Two arms extend west and south from the main body of the lake following the North Bosque and South Bosque Rivers. Two major tributaries, Hog Creek and the Middle Bosque River, enter the southern arm from the west. No major streams feed the lake from the east. The original dam was completed in 1929, and at an elevation of 430 ft, the lake covered ca. 2,700 acres. In 1965, a new dam was finished and the level raised to 455 ft, with the new conservation pool covering ca. 7,300 acres.

In January 1995, the East Texas Archeological Society (ETAS), the U.S. Army Corps of Engineers (COE), the U.S. Forest Service (USFS), and the Texas Historical Commission (THC) agreed to cooperate in a partnership project to inventory and monitor archaeological sites along the Lake Sam Rayburn shoreline. A Letter of Intent (LOI-095-02) was formulated and signed, stating that: the participants are mutually interested in fostering integrated problem solving among heritage resource managers regarding historic preservation issues, with special emphasis on training and information sharing. The Forest Service [and the COE] will gain additional information on the condition and location of archeological sites within its ownership along the shores of Sam Rayburn Reservoir. The participants will gain experience in recording and monitoring of site conditions, gain knowledge concerning the effects of shoreline erosion and looting of sites along the shores of Sam Rayburn Reservoir. Finally, the USFS and the COE will profit from the volunteer labor provided by members of ETAS. This paper reports on inventory and monitoring project activities that took place on September 30, 1995, and October 7, 1995 along the northern end of Lake Sam Rayburn. Elaine P. Sherman was the Project Archeologist, and the work was conducted under ARPA permit no. DACW63-95-0656.

The New Hope site (41FK107) is a prehistoric site located on a toe slope landform overlooking the Big Cypress Creek valley, the latter now inundated by Lake Bob Sandlin. An old creek channel lies about 100m to the east. Previous investigations reported by Nelson and Perttula indicate that the New Hope site covers about 2.5 acres, and was occupied during Late Paleoindian, Middle-Late Archaic, Woodland, and post-A.D. 800 Caddo times. The Caddo occupation was the principal occupation of the site. Nelson and Perttula noted at least 20-30 grave-sized looter holes there, and there have been reports that prehistoric burials have eroded out along the Lake Bob Sandlin shoreline. Two vessels in a private collection from the site are small bowls (5.2-5.5 em in height and 12.3-14.4 em orifice diameters) with tab tails on opposing sides of the vessel lip. One of the vessels has five horizontal engraved lines on the rim, and the other is plain.

The Earl Jones Farm site is one of a number of Late Caddo period, Titus phase (ca. A.D 1430-1680), sites along tributaries of Lake Fork Creek in the upper Sabine River basin in East Texas, nor far from Quitman, the county seat for Wood County. This includes sites such as J. H. Reese (41WD2), L. L. Winterbauer (41WD6), 41WD19, 41WD44, Pine Tree (41WD51), Burks (41WD52), and Steck (41WD529) with habitation features, midden deposits, and family cemeteries.

The three sites discussed in this article are within the Lake Wright Patman project area on the lower Sulphur River in East Texas. Two of the sites (Clayborn Springs [41BW55] and Mill Creek [41CS125]) are along the existing shoreline and flood pool, but Swen Farm (41BW65) is mostly submerged, except that the crest of the alluvial terrace the site is on is occasionally an island in the lake. All three sites have been eroded by wave action since the creation of Lake Wright Patman in the 1950s, and the Mill Creek site is still being looted.

The Pine Tree Farm site, an ancestral Caddo site occupied during the Titus phase (ca. A.D. 1430- 1680), was recorded by Bob D. Skiles in June 1977, on the basis of investigations conducted there by Skiles and James E. Bruseth, then a graduate student at Southern Methodist University, as well as work done by Skiles in 1970. The site is on a flat upland landform (400 ft. amsl) ca. 300 m northeast of Myrtle Springs Branch, a tributary to Dry Creek in the Lake Fork Creek drainage in the East Texas Pineywoods. The Goldsmith site (41WD208) is ca. 0.4 km to the east on Dry Creek, and several kilometers below the dam at Lake Quitman. The Dry Creek drainage basin, as well as the Caney Creek arm of Lake Fork Reservoir, have clusters of Late Caddo period Titus phase sites. The main known feature at the site was a large trash midden or midden mound that was ca. 20 x 22 m in size and approximately 35 cm in thickness. The midden deposits contained many Caddo ceramic vessel sherds, animal bones, mussel shell, charcoal and other charred plant remains (hickory nut shells), and ash deposits. Most of the trash midden was excavated in 1970, but there were intact midden deposits along the western and southern part of the trash midden, and in June 1977, several small units were excavated by Skiles and Bruseth in those areas. The ancestral Caddo ceramics discussed herein come from Units 2-4 in the southern part of the trash midden, but are not all of the sherds that were recovered in that work.

The Walter Bell site (41SB50) at Lake Sam Rayburn in the Neches–Angelina river basins in the deep East Texas Pineywoods was excavated by an National Park Service team in 1957. This was a small prehistoric Caddo farmstead or hamlet with two circular houses, a portion of a third house in the area of House 2, midden deposits, and six burials. Based on the kinds of artifacts found at the site (i.e., clay elbow pipes, a high proportion of brushed utility ware sherds from Broaddus Brushed vessels, and lower proportions of Pineland Punctated–Incised vessel sherds), the Walter Bell site was apparently occupied after ca. A.D. 1450–1500, in the Late Caddo period. Four of the burials (Burials 1–3 and 6) were in close association (either inside the house and underneath the house floor) with House 1, one (Burial 4) was inside House 2, and Burial 5 was in an open area, possibly a courtyard or work area between the two Caddo houses. Funerary offerings placed with the deceased included ceramic vessels, Perdiz arrow points, conch shell beads, deer ulna tools and deer food offerings, mussel shells, and engraved bird bone flageolets.

Many documented sites on the lower Sulphur River in the East Texas Pineywoods were occupied by Caddo peoples, and there are a number of such sites at Lake Wright Patman, including better known sites such as Knight’s Bluff (41CS14) and Sherwin (41CS26). These sites appear to have been small villages with family cemeteries, occupied between ca. A.D. 1200-1400. In this article, I discuss the ceramic sherd assemblages from three less well-known Middle Caddo period occupations at other sites at Lake Wright Patman.

The Steck site (41WD529) is a 15th to early 16th century A.D. Caddo settlement situated in the far western margins of the modern Pineywoods of East Texas, in the upper Sabine River basin in Wood County. The site is specifcally situated in the uplands more than 12m above the Dry Creek foodplain, in the upper part of the Lake Fork Creek drainage basin. Two natural springs emerge from the Queen City Eocene formation immediately below the site. There are two midden deposits at the Steck site, as well as evidence for structures arranged around an open plaza in a small community. The archaeological investigations reported on in this article took place in 1976 in a ca. 9 m diameter trash midden deposit along the edge of the upland landform; the trash midden was ca. 30 cm in thickness. Available notes and analysis records have been used to reconstruct what was accomplished at the site and the kind and range of recovered artifacts.

The distinctive Caddo ceramic vessels from the lower Neches–Angelina (i.e., Lake Sam Rayburn) and the lower Sabine (i.e., Toledo Bend Reservoir) river basins are not well understood, due to current cultural phase taxonomic difficulties and poorly defined ceramic assemblages. Sites in these areas were included in the Angelina focus by Jelks, which was a “broadly defined unit encompassing the entire Caddoan [sic] sequence in the Lake Sam Rayburn locality; needs reevaluation in light of larger sample of sites which are known in the area." Perttula used the term late Angelina focus to refer to sites in these localities that date after ca. A.D. 1400, but this taxonomic terminology is also not satisfactory. The vessels from the Lake Sam Rayburn Caddo sites warrant restudy, because currently it is difficult to determine what a representative assemblage of ceramic fine ware and utility ware vessels from this part of the Neches–Angelina River basin looks like, and the differing affiliations of local Caddo groups. Few of the vessels recovered from burials at Lake Sam Rayburn have been typologically identified. The rarity of Belcher Ridged sherds in the Lake Sam Rayburn sites when compared to their frequency in Toledo Bend Reservoir sites appears to indicate that the ancestral Caddo groups that once occupied these two areas had distinctly different utility ware traditions. Furthermore, stylistically–related Titus phase and Belcher phase engraved fine wares are absent in the Lake Sam Rayburn sites, much different from the Toledo Bend Reservoir ceramic assemblages, a trend which may be indicative of differing populations of Caddo peoples living in these two locales. The Lake Sam Rayburn ceramic assemblages warrant a thorough reanalysis—paired with radiocarbon dates from organics (and organic residues on sherds and vessels) in the collections—before it will be possible to establish their temporal, stylistic, and compositional character and diversity, and explore their relationship to other ancestral Caddo ceramic traditions in East Texas and Northwest Louisiana. This article summarizes the reanalysis of the Lake Sam Rayburn ceramic vessels. There are 21 vessels in the Texas Archeological Research Laboratory, The University of Texas at Austin collections from Lake Sam Rayburn archaeological investigations. This includes: 12 vessels from Walter Bell (41SB50); one vessel from Bird Point Islands (41SB71); one vessel from the Sawmill site (41SA89); six vessels from the Wylie Price site (41SA94); and one vessel from the Blount site (41SA123).

One of the more distinctive of the decorative methods represented in the ancestral Caddo ceramic assemblages from Lake Sam Rayburn sites is sherds and vessels with incised–punctated decorative elements. This diversity in the range and character of sherds and vessels with incised–punctated decorative elements is also the case in ancestral Caddo sites on the Sabine River and tributaries in the Toledo Bend Reservoir area of East Texas and Northwest Louisiana. Jelks included the incised–punctated vessels and sherds from the Lake Sam Rayburn sites in a newly defined type: Pineland Punctated–Incised. Pineland Punctated– Incised is a grog and/or bone–tempered utility ware, and occurs primarily as beaker–shaped jars as well as ollas and deep bowls. The vessels have concentric, triangular, rectangular, and curvilinear incised zones on the rim filled with tool punctation. Ollas and bowls have design elements on the vessel bodies. At Lake Sam Rayburn sites, Pineland Punctated–Incised sherds and vessels occur in both Middle Caddo (ca. A.D. 1200–1400/1450) and Late Caddo (ca. A.D. 1400/1450–1680) contexts. Based on the analyses discussed below, incised–punctated utility wares are most abundant in later Middle Caddo period components estimated to date from ca. A.D. 1300–1400/1450, and least common in post–A.D. 1400/1450 Late Caddo period components.

As the United States expanded in the late eighteenth century and through most of the nineteenth century, much interest and question was raised over the increasing numbers of earthen mounds and earthen constructions encountered by the settlers moving westward across the southeastern woodlands. Mounds? Mound builders? Enough questions were raised about their origins that in 1881, the Division of Mound Exploration of the Bureau of Ethnology, Smithsonian Institution, was established to address and resolve these issues. The work of the Division of Mound Exploration can be considered the first "modern archeology" done in the United States. Their mound research covered the Dakotas to Texas and all points east. The final research report by Division Head, Cyrus Thomas, was published as the Twelfth Annual Report of the Bureau of Ethnology. In this report, Thomas mentions in the Gulf District that: some two or three mounds of peculiar form have been discovered in Mississippi and the Arkansas district that have not been observed elsewhere in the mound area. These may be described as earthen platforms surmounted by a conical mound or a conical mound surrounded by a terrace. Sometimes the conical mound is small in proportion to the platform and is not central...A double mound of this type, or mound with two apices, has been observed in western Mississippi. The primary purpose of this report is to make known the occurrence of a two-phase Caddoan earthen mound in Upshur County. Furthermore, this report seeks to add this site to the inventory of known archeological resources of the Cypress Creek basin. Available data relevant to the Cypress Basin and the immediate area of the site has also been summarized and reported here to suggest chronological associations for the two-phase mound.

Sometime around ca. A.D. 800, Lake Naconiche sites were no longer occupied by Woodland period groups of the Mossy Grove culture solely making sandy paste pottery or living as mobile hunting-gathering foragers. At this time, from ca. A.D. 750-800 to around A.D. 900, colder and drier conditions began to dominate the local weather. After ca. A.D. 800, were the aboriginal groups Caddo peoples or acculturated Mossy Grove folks? Some findings from the Lake Naconiche archaeological investigations at the Boyette site (41NA285) are relevant to this issue of ethnic affiliations and local, but nevertheless regional momentous, cultural changes. Putting that in context, as best as can be discerned in the archaeological records of the Woodland period occupations at the Naconiche Creek (41NA236) and Boyette sites, if there is any evidence of increasing sedentism, it is only apparent after ca. A.D. 400 or perhaps even as late as ca. A.D. 650, during the latter part of the period. Even so, these occupations were not sedentary in the sense of them being year-round occupations (as with the Caddo settlement history at Lake Naconiche) or even multiseasonal occupations. The sites do not have accumulations of midden deposits, there is no evidence for the construction of sturdy wood structures, and there are only a very modest assortment of burned rock, pit, or post hole features at the Woodland period sites. It is hard to disagree with Story’s characterization of Woodland period settlements in the general area that they reflect “intermittent encampments by a relatively small group or groups over a considerable period of time.” Woodland period sites are widely distributed on many different kinds of landforms, implying the generalized use of a wide variety of habitats for settlements as well as foraging pursuits. Without a more fine-grained Woodland period chronology for Mossy Grove culture sites in East Texas, which we are a long way from achieving, it is not possible to evaluate suggestions by Corbin that there were subtle shifts on the landscape of peoples that may have been a response to changes in subsistence (i.e., the possible growing of cultivated plants). The absence of cultigens other than squash from Woodland contexts in the Lake Naconiche paleobotanical record casts some doubt on the assertion that horticultural economies were developed during this time locally, although the number of flotation and fine-screen samples from pre-A.D. 800 contexts is still miniscule. Thus, the virtual absence of cultigens from Woodland times does not yet constitute a robust evaluation of Corbin’s suggestion. The development of sedentary life along Naconiche Creek appears to have taken place after ca. A.D. 800 by successful hunter-gatherer foragers and pottery makers, specifically amongst the earliest Caddo residents of the valley. Neither the adoption of pottery or the adoption of horticultural subsistence strategies (i.e., the cultivation of maize) appear to have been triggering events that led to the ability of these people to maintain multi-seasonal residences in the same places.

1. In this paper, I want to begin to contemplate the possibility that the concept of culture could one day be thought outside modern Western thought, via a reading of Martin Heidegger's 'Dialogue on Language between a Japanese and an Inquirer'. As we shall see, for Heidegger, the dominant position here is representationalism. And so a large part of what I want to do here is to begin to shake the concept of culture from these dominant representationalist moorings.1 Heidegger's problem with the history of Western thought may be put as follows. In this tradition, the difference between Being and beings (the ontological difference) is forgotten so that Being comes primarily to be considered in terms of beings. Beings are, in turn, considered in terms of their relations to one another, with the being called 'man' routinely standing to one privileged side of those known as 'objects'. Thereby thinking about Being is reduced to the question: how is it that man relates to objects? The problem is compounded when the answer to this question is given as: man knows objects. And it is even further compounded by the dominance throughout all of this of representational thinking: the idea that man knows objects only 'indirectly' through their representations.2 To challenge representational thinking in Heidegger's sense, then, is to challenge not just a 'way of knowing' but also the dominance of 'man' (Foucault's central uptake of Heidegger), the separation between 'man' and 'things', subject and object, and, ultimately, it is to challenge the very idea that Being is no more than the aggregate of empirically accessible beings. To find alternatives to representational thinking Heidegger looks elsewhere, or, more precisely, in turning to the pre-Socratic Greeks, elsewhen. He does this throughout his work, but most explicitly in Early Greek Thinking. Yet even this work barely distinguishes between (what we would now think of as) natural and cultural production in any clear way. Instead, it appears there as if the ontological difference itself -- the difference between Being and beings, between sheer coming-to-presence and that which happens to be present -- is of such urgent importance that it cuts across the apparently less important distinction between natural and cultural varieties of beings. As an advancement of his claims about the ontological difference (as a neglected and almost unthinkable difference today), the Heidegger of Early Greek Thinking in effect obviates the nature/culture distinction along with representational thinking. If the modern Western concept of culture, then, depends for its existence on the prior existence of a constitutive outside (such as nature) then is it possible that culture as such (whatever theory of it we hold) is irrevocably part of representationalist thinking? Is it intrinsically representationalist -- from, say, Hobbes to the present day or, indeed, in whatever past or future manifestation -- by virtue of its dependence on a culture/non-culture distinction? If this is so, again, there is a remarkable consequence for all the cultural disciplines and for cultural studies in particular. It is this: any non-representationalist approach to culture would be a contradiction in terms; so that, by virtue of it being specifically culture we are interested in, our interest will be necessarily representationalist. Outside representationalism, what we are dealing with could not be culture as such. The sorts of objects which we have, until now, thought of as cultural objects (photographs, museums, policy documents, forms of dress, music and so on) become interesting and significant outside representationalism only to the extent that they instanciate the ontological difference. We can, that is, no longer afford to think of the cultural as ontologically separate in any way. Instead, the move away from representational thinking would mean that objects of whatever kind -- 'gods and men, temples and cities, sea and land, eagle and snake, tree and shrub, wind and light, stone and sand, day and night' (Early 40) -- are effects of the distinction between coming-to-presence and merely happening to be present. And they ought to be experienced, inspected and understood for what they are, fundamentally, in this respect. What would this mean? One occasion where the later Heidegger does treat 'cultural' matters (in several senses and by, perhaps for the first and only time, going across contemporary cultures) is in his 'Dialogue on Language between a Japanese and an Inquirer'.3 This strangely readable little colloquy requires inspection, I believe, if we are to proceed any further along what the Heidegger of Early Greek Thinking calls 'the lines of usage' and into the peculiar territory beyond representational thinking.4 2. What is at stake here may be this: whether in this dialogue we are experiencing a cultural difference. Or perhaps, whether we are experiencing the presence of culture(s) at all. Put another way: the essay in question (in the form of a dialogue) may be an instance of either (a) a cultural difference or dialogue; or else of (b) an accidental similarity or monologue. Let us look briefly at both possibilities. If (a), then we have a clear lesson (on the model of the 'danger' of language): the nature of culture cannot derive from a metaphysical distinction between culture and contenders for the title of 'non-culture' (for example, physical nature or science or barbarism); for anything that was a culture would have to be already in place in order to generate such a division (or anything like it) in the first place. The name, then, of this prior condition cannot be 'culture' itself. But that is precisely how the cultural disciplines have used the term -- 'cultures', we hear, are what make the 'nature/culture' distinction -- albeit that each may do it in its own, 'culturally specific', way.5 This is why the cultural disciplines cannot imagine a culture which does not, in itself, have a deeply-seated representational concept of culture as its ultimate ground. Anthropological thinking -- in the broadest philosophical sense, not just in reference to a specific discipline -- entails the search for the other's meaning of its own (anthropology's) idea of culture. In effect, it cannot imagine a culture outside Western metaphysics but must forever translate 'different cultures' into versions of it (albeit with minor empirical differences). So if the name of the condition for the nature/culture distinction (that is, the name of the nature of culture) cannot be 'culture', instead its name must be 'representational thinking' -- at least for all cultural theory to date. The modern concept of culture's ultimate contradiction would be that it would rest upon the assumption of its own universal presence while also denying cultural universals. If (b) -- if, that is, we are not, in this dialogue, undergoing an experience with culture at all -- then the 'Japanese' is no more than a token non-European Heideggerian. He is whatever may be non-European 'in' Heidegger himself. He is a fictional device for having Heidegger's fellow Europeans (his readers) see how it is possible to think outside Western thinking -- or at least to get a glimpse of such a possibility. He is a stooge, a ploy, and -- what's more -- an 'orientalised' ploy: a classically European fictional depiction of the mysterious Orient and its inscrutable thinking. So much is at stake in how we read this work. And a number of very important issues depend on our (necessarily ethical and political) decision as to how we should read the essay. For if reading (a) prevails, cultural difference (or whatever term we decide to use to replace it and its ultimately limited horizon) is not something, in itself, of its nature or essence, that can give any comfort to notions of 'orientalism' or 'stereotypes' or any of the other tropes of fashionable (cross-)cultural criticism. Instead, the cultural itself, wherever it is predicated on Western representational thinking, is intrinsically Western thinking. There is no outside of Western culture (the Western concept of culture) for that culture to grasp -- whether it would ideally grasp it in scientific, anthropological, liberal humanist, cultural relativist, orientalist, colonialist or racist ways. These 'ways' and the differences between them have no meaning on reading (a). They are all, in effect, one way. But if reading (b) prevails, then all seems well with Western representational thinking. It has no problem because, now, all cultures would, factually, have a Western concept of culture at their core, albeit of a particular inflexion. They would all be just like 'us' in their essential metaphysics. He who recorded the different tensions or versions of this single metaphysics might be a scientist or anthropologist. He who appreciated such small variations might be a liberal humanist or a cultural relativist. He who dogmatically believed in the superiority of his own tension or difference and degraded others might be an orientalist, a colonialist or a racist. But these would be, on reading (b), but small variations along a single path. They would be like the right, left and centre lanes of a one-way street. So neither reading turns out to be very hopeful for today's cultural disciplines. The first suggests a much deeper-seated difference than those disciplines have been able to imagine hitherto; something much less easily grasped than the culture/non-culture distinction (and such that some 'cultures' are not, in and of themselves, quite that). The second suggests that the easy victories of principled cultural criticism and cultural identity politics (as well as those of less 'enlightened' positions) are grounded on the most Western of Western thinking: its representationalist theology.6 It looks as if there are only two possibilities: either culture rests upon a bed of difference that lies so deep as to remain forever outside Western thinking; or every other is ultimately, at the deepest point of difference we can think, a version of the West. But on both sides of the divide, the initial idea of culture is culture-as-presence: 'are we in the presence of an intercultural dialogue?'; or 'are we in the presence of a culture talking to itself?' If we could move even a little way from this and begin to think of culture-as-coming-to-presence (or just as 'to come', to invoke a Derridean variation on the theme), then it turns out that (a) and (b) are necessarily undecidable matters within representational thinking itself but that, as we begin to move outside it,the decision becomes irrelevant. But we must reserve this (in)decision for another occasion and proceed with the dialogue at hand. 3. To proceed, we must continue with the dialogue's attention to language and particularly to the 'danger' of speaking about it. Language, that is, has a nature but it is concealed (by the representationalist difference between the sensuous and the suprasensuous) and this concealing is a 'danger' (21). One contender for the nature of language is to take it as 'the house of Being' (22). And this prompts us to remember that the dialogue describes the two cultures as different 'houses' (5) -- different 'language realities' (24) -- so that 'the nature of language remains something altogether different for the East asian and the European peoples' (23). In fact, it is so different that the question of what language is may not be a possible one for the Japanese (23). He insists that his people 'pay no heed' to the question of the nature of language. Instead they have a word that 'says the essential being of language, rather than being of use as a name for speaking and for language' (23). So this is not a referring word but rather a 'hinting' word (24). And the 'hint' would be what the Japanese translator feels when he feels the 'wellspring' from which such different languages as German and Japanese might arise. He also describes this in terms of a 'radiance'. This 'hinting', or 'gesturing', or 'bearing' (26) must not, the Inquirer demands, be clarified into a form of 'conceptual representation' (25). Were it to be, we would miss its nature outside Western reason. There is no analytic or empirical equivalent of 'the nature of language'. To think so is itself an instance of the worst sorts of metaphysics at work. Following through the dialogue, we also find that to ask about the nature of language is also to ask the hermeneutic question in its non-standard sense;that is not as a methodological question about the means of interpreting texts but as a metaphysical question about what interpretation itself is (29-30). And this in turn has to do with 'bearing' (as in bearing a message, being a messenger -- gesturing, bearing, hinting). The so-far unannounced Japanese word for the nature of language, on the one hand, and the question of what hermeneutics is, on the other, stand together. 'Man stands in hermeneutical relation to the two-fold' (32), where 'the two-fold' is glossed as presencing (coming-to-presence) and present beings. This hermeneutic relation, however, is complex. It involves man in preserving the two-fold (32) and also in the two-fold (presencing/present) using man (33). And, obviously enough perhaps, this idea of 'use' can no longer mean empirical usage in its quasi-linguistic sense. For, as we soon learn from the rest of the essays in On the Way to Language, the linguistic arts and sciences are thoroughly representationalist since they begin with the assumption of the simple existence of present beings (forgetting coming-to-presence and language's criticality to it) and consider language, as it were, to come later as a means of, and for, their re-presentation. (And this is, I would argue, precisely the function of terms such as 'language', 'discourse', 'signification' and 'image' in, for example, cultural studies.) Nevertheless the alternative to this mistaken view of language, the alternative that Heidegger calls 'the hermeneutic relation', is agentive. In fact it is doubly so. It involves, that is, practices (of preserving and using): 'the sway of usage' (33) and 'the sway of the two-fold' (34). The Japanese claims that there is a kinship between this thinking and his (or their) own (41). This hermeneutic relation, however, is complex. It involves man in preserving the two-fold (32) and also in the two-fold (presencing/present) using man (33). And, obviously enough perhaps, this idea of 'use' can no longer mean empirical usage in its quasi-linguistic sense. For, as we soon learn from the rest of the essays in On the Way to Language, the linguistic arts and sciences are thoroughly representationalist since they begin with the assumption of the simple existence of present beings (forgetting coming-to-presence and language's criticality to it) and consider language, as it were, to come later as a means of, and for, their re-presentation. (And this is, I would argue, precisely the function of terms such as 'language', 'discourse', 'signification' and 'image' in, for example, cultural studies.) Nevertheless the alternative to this mistaken view of language, the alternative that Heidegger calls 'the hermeneutic relation', is agentive. In fact it is doubly so. It involves, that is, practices (of preserving and using): 'the sway of usage' (33) and 'the sway of the two-fold' (34). The Japanese claims that there is a kinship between this thinking and his (or their) own (41). Footnotes Representational thinking is clearly alive and well today in cultural studies -- perhaps even to the point whereby this otherwise critical discipline rarely subjects this concept to critical scrutiny. See Hall (Representation). A draft paper 'Representation and Cultural Studies' (available on request) deals with this question. Hall's Representation book lists three such forms of indirect representation: 'the production of meaning through language, discourse and image'. Two other central locations for Heidegger on culture are 'The Age of the Word Picture' and 'Science and Reflection'. Here and elsewhere, of course, Heidegger has very little time for the idea of culture and 'culturalist' explanations -- possibly because of their traditionally deep imbrication in representationalism. At times, his opposition is so vehement that we can practically hear him reaching for his gun. In Early Greek Thinking, Heidegger translates a crucial part of the Anaximander fragment as follows: '... along the lines of usage [custom, practice]: for they let order and thereby also reck belong to one another (in the surmounting) of disorder' (Early 57). A paper submitted for this issue of M/C nicely displays this in a single phrase: 'the Western cultural pattern that assigns things masculine to the cultural and things feminine to the natural' (my emphases). On this matter, see Hunter on 'Setting Limits'. References Hall, Stuart (ed). Representation: Cultural Representations and Signifying Practices. London: Sage, 1997. Heidegger, Martin. "The Age of the Word Picture." Trans. W. Lovitt. The Question Concerning Technology and Other Essays. New York: Garland, 1977. 115-54. ---. "A Dialogue on Language between a Japanese and an Inquirer." Trans. P.D. Hertz. On the Way to Language. San Francisco: Harper & Row, 1971. 1-54. [First German publication 1959] ---. Early Greek Thinking. Trans. D. Farrell Krell & F.A. Capuzzi. New York: Harper & Row, 1975. ---. "Science and Reflection." Trans. W. Lovitt. The Question Concerning Technology and Other Essays. New York: Garland, 1977. 155-82. Hunter, Ian. "Setting Limits to Culture." Nation, Culture, Text: Australian Cultural Studies. Ed. G. Turner. London: Routledge, 1993. 140-63. Citation reference for this article MLA style: Alec McHoul. "Talking (across) Cultures: Grace and Danger in the House of the European Inquirer." M/C: A Journal of Media and Culture 3.2 (2000). [your date of access] <http://www.api-network.com/mc/0005/grace.php>. Chicago style: Alec McHoul, "Talking (across) Cultures: Grace and Danger in the House of the European Inquirer," M/C: A Journal of Media and Culture 3, no. 2 (2000), <http://www.api-network.com/mc/0005/grace.php> ([your date of access]). APA style: Alec McHoul. (2000) Talking (across) cultures: grace and danger in the house of the European inquirer. M/C: A Journal of Media and Culture 3(2). <http://www.api-network.com/mc/0005/grace.php> ([your date of access]).