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Rojas, Fernandez Alejandro [Verfasser]. "Regulation of Hdm2, HdmX-mediated ubiquitination and neddylation / Alejandro Rojas Fernandez." Konstanz : Bibliothek der Universität Konstanz, 2010. http://d-nb.info/1024034968/34.
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Mpagi, Meldrick Daniel. "In Search For New p53 Regulated Genes." Wright State University / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=wright1227282714.
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Heminger, Katherine Ann. "LOSS OF HDMX LEADS TO ALTERATIONS IN GENE EXPRESSION AND INHIBITION OF CELL GROWTH IN TUMOR CELLS WITH WILD-TYPE p53." Wright State University / OhioLINK, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=wright1175282827.
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Jani, Meghna. "Transcriptional regulation of LAMB3 by p53." Wright State University / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=wright1229958362.
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Nystøyl, Bjarte Løken. "HDMI Transmitter." Thesis, Norges teknisk-naturvitenskapelige universitet, Institutt for elektronikk og telekommunikasjon, 2012. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-18483.
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HDMI is the de facto global standard for connecting HD components and bridging the gap between consumer electronics and personal computer products, making it a priority to develop efficient hand-held, battery-powered units that support the standard.This is a study into how to design a low power and high performance system that can transmit HDMI-signals to a valid HDMI-receiver. The main priority is to implement the TMDS part of a HDMI-transmitter, where parallel data is encoded and serialized at high frequencies. The theory chapters provides an orderly summary of the complex workings of the HDMI-standard, in addition to an introduction to high-performance digital circuit design. This is followed by a system specification chapter, which sets the constraints of the design and discusses the hardware requirements. The subsequent chapter first deals with the design of a straightforward, basic HDMI-transmitter, before moving on to an enhanced design process. The basic design is used as a base for discussions in regard to how effective the suggested enhancement techniques are. The improvements result in an enhanced design able to operate at 742,5 MHz and support High-Definition video at the impressive resolution of 1080p30. This is achieved by using a 180nm, low-leakage library, and the final design consists of approximately 24.000 unit-sized transistor equivalents, consuming approximately a total of 13,6 mW.
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Gu, Xun. "HDMI frame grabber." Thesis, California State University, Long Beach, 2014. http://pqdtopen.proquest.com/#viewpdf?dispub=1526915.
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The High-Definition Multimedia Interface (HDMI) is widely adopted by the modern media devices, and supporting HDMI source is required in many streaming and broadcasting solutions. This paper introduces an HDMI frame grabber design which will capture the image from an HDMI source, and then display the image on both a local HDMI display and a remote computer screen. As a result, the captured frame will be shared in the different locations across the network. The project design involves the embedded hardware/software development on a Xilinx FPGA board, and GUI/Network programming on the Microsoft .NET framework. The paper describes the embedded design to enable the HDMI and Ethernet peripherals on the FPGA board, analyzes the data format and protocol difference among the network nodes, and then introduces the method to convert and transport the data.
Keywords: Embedded Design, FPGA, HDMI, lwIP, Network Programming.
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Yu, Grace Wang. "Characterization of HDM2 domains." Thesis, University of Cambridge, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.613861.
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Čurda, Pavel. "Měřicí přístroj HDO signálů." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2019. http://www.nusl.cz/ntk/nusl-400610.
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The thesis deals with the introduction of the measuring instrument SVA - M on the market for compliance with electromagnetic compatibility and electrical safety requirements. Furthermore it deals with determination of measuring accuracy instrument SVA - M and measurement uncertainties. For the determination of accuracy there is a theoretical analysis and subsequent practical measurements.
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Bartek, Tomáš. "Řídící obvod s rozhraním HDMI pro modulární LED displeje." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2016. http://www.nusl.cz/ntk/nusl-242003.
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This work deals with modernization of information LED panels. It mainly focuses on utilizing input HDMI interface into FPGA, which controls modular LED displays, but also on ensuring professional functions such as communication with control unit, thermal security and detection of faulty LED.
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Kaltea, Eddie, and Daniel Lundgren. "Utveckling och konstruktion av analysatorverktyg för styrsignaler i HDMI-gränssnittet." Thesis, Linköping University, Linköping University, Computer Engineering, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-16789.
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Utveckling av produkter som skall stöda HDMI-standarden medför många hinder som behöver överkommas. Ett av problemen är certifiering mot standarden. Det är svårt att testa att standardens alla krav uppfylls på ett utvecklingsföretag då testutrustningen är kostsam och därför ej tillgänglig. Ett enkelt verktyg har därför utvecklats för att underlätta testning av att standarden följs.
Denna rapport inleds med en problemställning och grundläggande teori om relaterade ämnen. En förstudie följer sedan där olika sätt att lösa problemet presenteras. Sedan följer en övergripande beskrivning om hur verktyget fungerar och hur det tillverkades. I slutet på rapporten finns en efterstudie och resultat som beskriver hur verktygets utveckling har fungerat och hur resultatet från förstudien påverkat utvecklingen.
Resultatet av examensarbetet är en prototyp som går att använda för att underlätta testning av att HDMI-standarden följs i vissa avseenden.
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Mirnezami, Alexander H. F. "Investigation of a novel protein : protein interaction involving the hdm2 oncoprotein." Thesis, University of Southampton, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.399993.
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Miller, Kelly Lynn Robbins. "Induction of p53 Dependent Cellular Senescence Through HdmX Inhibition or YPEL3 Expression." Wright State University / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=wright1276791515.
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Nunes, Diego Frinhani. "Procedimento para análise de sensibilidade do Programa HDM-4." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/18/18143/tde-20062012-160119/.
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A presente dissertação teve como objetivos principais delinear um procedimento de análise de sensibilidade que possa ser utilizado de forma ampla por usuários do programa computacional de gerência de pavimentos HDM-4 e analisar a influência de modificações no cenário pré-definido no estudo de caso, notadamente considerações de projeto e de tráfego. A escolha do Método dos Efeitos Elementares foi precedida por uma revisão bibliográfica sobre análise de sensibilidade, sendo que o procedimento foi delineado com um estudo de caso que partiu de um cenário pré-definido (rodovia de pista simples e pavimento asfáltico sobre base granular) e fez a análise da variação de 61 fatores, em 300 trajetórias, totalizando 18.600 simulações no programa HDM-4. Os resultados deste estudo indicaram que a utilização de uma amostra com 30 trajetórias é adequada, pois aumentar este número não acarreta ganhos significativos e a sua diminuição ocasiona prejuízos. Com o procedimento delineado, passou-se à verificação de impactos que a modificação dos cenários pré-definidos para a análise gerariam, ou seja, se a modificação de fatores que não estão no grupo de 61 fatores em estudo interferiria no resultado final do procedimento. Foram estudadas modificações nas considerações de projeto (Taxa de Desconto Monetário Anual e Período de Análise) e nas considerações de tráfego (Percentual de Veículos Comerciais e da Taxa de Crescimento Anual do Tráfego), com os resultados evidenciando que ambas as modificações influem no resultado final da análise de sensibilidade, sendo que as modificações nas considerações de tráfego se mostraram mais influentes que as modificações nas considerações de projeto. Complementarmente, foi feita uma análise de sensibilidade pelo Método dos Efeitos Elementares, com 250 trajetórias, que identificou como os três fatores mais influentes na análise econômica do programa HDM-4, dentro do universo estudado, o Volume Diário Médio Anual de Veículos Motorizados, o Fator de Calibração para a Progressão da Irregularidade Longitudinal e o Fator de Calibração para a Razão do Número Estrutural Ajustado das Estações Úmida e Seca.This Master of Science Dissertation aimed to outline a procedure for sensitivity analysis that can be widely used by computer program of pavement management HDM-4 users. It also aimed to analyze the influence of changes in the pre-defined scenario of the case study, especially design and traffic considerations. The choice of the Elementary Effects Method was preceded by a literature review on sensitivity analysis, and the procedure was outlined with a case study that came from a pre-defined scenario (two lane standard and asphalt pavement on granular base) and variations of 61 factors, considering 300 trajectories, in a total of 18,600 simulations in HDM-4 program. The results indicated that the use of a sample with 30 trajectories is appropriate, because increasing this number does not cause significant gains and reducing it can harm the analysis. With the procedure outlined, it was checked if changes in factors that are not in the group of 61 studied factors can interfere with the end result. Thus, changes in design (Discount Rate and Period Annual Monetary Analysis) and traffic (Percentage of Commercial Vehicle and Annual Growth Rate of Traffic) considerations affected the final result the sensitivity analysis, and the changes in traffic considerations were more influential than changes in design considerations. In addition, it was conducted a sensitivity analysis by the Elementary Effects Method, with 250 trajectories, which identified as the three most influential factors in the economic analysis of HDM-4 program, within the universe studied, the Annual Average Daily Traffic of Automobiles, the Calibration Factor for the Progression of Longitudinal Roughness and the Calibration Factor for the Ratio of Adjusted Structural Number Wet and Dry Seasons.
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Cui, Xiaoyun. "Dynamics of histone modification in integration of metabolic activity and stress response." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLS474.
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Le réchauffement climatique a des effets importants et coûteux sur le climat et la production agricole. Étant sessiles, les plantes ont développé des mécanismes complexes pour percevoir les variations de température et y répondre, ainsi que pour afficher des changements spécifiques dans leur morphologie ou leur développement. Cependant, on sait peu de choses sur le mécanisme de reprogrammation de l'expression génique de la chromatine lors de la réponse de la plante à une température ambiante élevée. Les enzymes de modification de la chromatine nécessitent des produits de métabolisme intermédiaire en tant que substrats ou cofacteurs. Par exemple, les histone acétyltransférases utilisent l’acétyl-CoA en tant que donneur pour l’acétylation de la lysine et les histones déméthylases nécessitent l’a-cétoglutarate (α-KG) en tant que cofacteur. Chez les plantes, l'acétyl-CoA cytosolique est produite par l'ATP-citrate lyase (ACL) et l'α-KG est produite par les isocitrate déshydrogénases (ICDH) par différentes voies métaboliques. Cependant, il reste à déterminer si les fluctuations métaboliques affectent l'activité des enzymes de modification de l'histone et régulent l'expression des gènes lorsque la température ambiante est élevée. Dans ma thèse, j'ai contribué à analyser le rôle des histone désacétylases HDA9, HDA15 et HDA19 d'Arabidopsis dans la réponse thermique des plantes et j'ai étudié l'effet des niveaux d'acétyl-CoA sur l'état d'acétylation des histones chez Arabidopsis et le rôle de la fluctuation de l'α-KG dans le contrôle Activité de la déméthylase JmjC et croissance de la plante et expression génique à température chaude. Dans la première partie de l’étude, des analyses génétiques et moléculaires ont montré que HDA9, HDA15 et HDA19 s’adressent à différents ensembles de gènes et jouent des rôles distincts dans la réponse à la chaleur. D'autre part, nous avons constaté que lorsque le ACL était surexprimé chez les mutants gcn5, le niveau de H3K27ac était augmenté. De plus, la surexpression des ACL complétait partiellement les phénotypes gcn5. Les résultats ont démontré un lien intrinsèque entre les fluctuations de l'acétyl-CoA et les niveaux d'acétylation de l'histone H3K27 chez les plantes. Dans la deuxième partie de ma thèse, nous avons montré que la perte d’ICDH cytosolique entraînait une augmentation des niveaux de H3K4me3 et une augmentation des phénotypes mutants du gène de la déméthylase H3K4me3, JMJ14. Les analyses génétiques ont suggéré que JMJ14 et JMJ15 (une autre déméthylase H3K4me3) fonctionnaient de manière redondante pour réguler l’expression et la croissance de gènes sensibles à la chaleur des plantes et indiquaient que la mutation de cICDH affectait principalement la fonction de JMJ15. Une analyse pangénomique a révélé le rôle essentiel de JMJ14 dans l'établissement de programmes d'activation et de répression géniques de la thermomorphogenèse des plantes. JMJ14 et JMJ15 ont directement réprimé un ensemble de gènes susceptibles de jouer un rôle négatif dans le processus. Les résultats ont montré que la fluctuation des taux de métabolites régulait l'activité de l'histone déméthylase et la réponse des plantes à la chaleur. Pris ensemble, les résultats mettent en évidence l'interaction entre le métabolisme, l'épigénétique et l'adaptation des plantes au changement de l'environnement ambiantGlobal warming is having significant and costly effects on the climate and agricultural production. Being sessile, plants have evolved complex mechanisms to perceive and respond to temperature variation and display specific changes in their morphology or development. However, little is known on the chromatin mechanism of gene expression reprogramming during plant response to elevated ambient temperature. Chromatin modification enzymes require intermediary metabolism products as substrates or cofactors. For example, histone acetyltransferases use acetyl-CoA as a donor for lysine acetylation and histone demethylases require α-ketoglutarate (α-KG) as a cofactor. In plants, cytosolic acetyl-CoA is produced by ATP-citrate lyase (ACL) and α-KG is produced by isocitrate dehydrogenases (ICDH) in different metabolic pathways. However, it remains unclear whether metabolic fluctuation affects the activity of histone modification enzymes and regulates gene expression under elevated ambient temperature. In my thesis, I contributed to analyze the roles of Arabidopsis histone deacetylases HDA9, HDA15, and HDA19 in plant thermal response and I studied the effect of acetyl-CoA levels on histone acetylation status in Arabidopsis and the role of α-KG fluctuation in controlling JmjC demethylase activity and plant growth and gene expression under warm temperature. In the first part of the study, genetic and molecular analysis showed that HDA9, HDA15, and HDA19 target to different sets of genes and play distinct roles in responding to warm temperature. On the other hand, we found that when overexpressed ACL in gcn5 mutants, the H3K27ac level was increased. Additionally, ACL overexpression partially complemented the gcn5 phenotypes. The results demonstrated an intrinsic link between acetyl-CoA fluctuation and histone H3K27 acetylation levels in plants. In the second part of my thesis, we showed that loss of cytosolic ICDH resulted in increased H3K4me3 levels and enhanced mutant phenotypes of the H3K4me3 demethylase gene JMJ14. Genetic analysis suggested that JMJ14 and JMJ15 (another H3K4me3 demethylase) functioned redundantly to regulate plant thermal responsive gene expression and growth and indicated the cICDH mutation mainly affected JMJ15 function. Genome-wide analysis revealed an essential role of JMJ14 in establishing both gene activation and repression programs of plant thermomorphogenesis. JMJ14 and JMJ15 directly repressed a set of genes that are likely to play a negative role in the process. The results provided evidence that the fluctuation of metabolites levels regulates histone demethylase activity and plant response to warm temperature. Taken together, the results highlight the interplay between metabolism, epigenetics and plant adaptation to changing the ambient environment
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Mathie, Sara A. "Mechanisms underlying the resolution of HDM induced allergic airways disease." Thesis, Imperial College London, 2013. http://hdl.handle.net/10044/1/29879.
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Allergic asthma is a chronic inflammatory disease of the lung and deficiencies in pro-resolving mechanisms may contribute to the persistence of inflammation. The overall aim of this project was to establish a resolution model of house dust mite (HDM) induced allergic airway disease (AAD) and identify mediators of resolution. In our model, features of disease, induced by HDM at peak disease 4 hours, airway hyper-reactivity (AHR), Th2 lymphocytes and eosinophils remained significantly elevated 7 days after last challenge, resolving to baseline by 13 days. The levels of FoxP3+ regulatory lymphocytes also follow this pattern. However, as disease waned there was an elevation in the levels of alveolar macrophages and up regulation of the homeostatic molecule CD200R up to 13 days. Exposure to a single i.n administration of HDM in the resolved airways resulted in a rapid increase in Th2 inflammation and AHR suggesting that after resolution of HDM inflammation there is altered immune homeostasis in the lung. The pro-resolving lipid Lipoxin A4 was induced in the lung by HDM exposure and remained detectable during resolution. Depletion of alveolar macrophages during the resolution phase of allergen challenge resulted in delayed clearance of Th2 lymphocytes, airway neutrophils and interstitial macrophages. Conversely, adoptive transfer of alveolar macrophages during resolution resulted in reduced numbers of lung tissue leukocytes, specifically neutrophils and interstitial macrophages. This suggests a cross talk between these macrophage subsets and a novel interaction for pulmonary homeostasis. The anti-inflammatory peptide Annexin A1 is highly expressed by alveolar macrophages and mice deficient in Annexin A1 had enhanced AHR and Th2 immunity response to HDM. Blocking the Annexin A1 receptor FPR2 enhanced AHR and lung inflammation. Conversely, therapeutic administration of an Annexin A1 mimetic improved AHR and Th2 immunity. These studies demonstrate that Annexin A1: FPR2 pathway may be important in HDM disease and that resolution of allergic airways disease is an active process resulting in altered homeostasis of the lung.
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Bartel, Frank. "Molekulare Charakterisierung von HDM2 (MDM2) in Weichteilsarkomen - Bedeutung als Prognosefaktor und Ziel innovativer Therapiestrategien." [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=964346419.
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Schmerr, Martin J. "THE FUNCTIONAL SIGNIFICANCE OF AN ALTERNATELY SPLICED PRODUCT OF THE HDM2GENE." Ohio : Ohio University, 2007. http://www.ohiolink.edu/etd/view.cgi?ohiou1173370332.
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Liu, Yan. "The Roles of Two Different Pathways in Hypoxia: p53/HDM2 and PERK/GCN2/eIF2α." Ohio University / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1249582516.
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Steiner, Esther Susanne. "OPAC 2.0 mit Web 2.0-Technologie zum Bibliothekskatalog der Zukunft? /." Stuttgart : Hochschule der Medien, 2007. http://opus.bsz-bw.de/hdms/volltexte/2007/624/pdf/steiner_bachelorarbeit.pdf.
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Hakimi, Amirmansoor. "Plasma protein profiling for bladder cancer biomarker discovery using UPLC-HDMS^E label-free quantitation." Thesis, University of Leicester, 2013. http://hdl.handle.net/2381/28260.
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In the UK, bladder cancer is the 4th most common cancer in men and 11th most common in women. In 2010, just over 10,000 new cases were diagnosed and 4,900 deaths were recorded. At their first diagnosis, the majority of bladder cancer patients (75-85%) present with non-muscle invasive disease. In 50-70% of these patients the tumour will recur and in 10-20% of them it will progress to muscle invasive disease. Mass spectrometry based proteomics has been chosen for clinical biomarker discovery due to its ability to perform qualitative and quantitative protein profiling on clinical samples. In total 90 plasma samples were used in this study in two groups of disease and control. An optimised and evaluated UPLC-IMS-DIA-MSE label-free quantitation method was used for plasma protein profiling. To our knowledge, this is the first report investigating the biomarkers of bladder cancer incorporating label-free quantitation and UPLC-IMS-DIA-MSE methodology. To assess expression level of proteins of samples in different groups a plan consisting of four data processing packages was used. Each of the packages uses different statistical means by which to identify proteins and/or compare expression levels alteration. Optimisation of the methodology helped in the thorough investigation of the plasma proteome with coverage of up to five orders of magnitude of plasma protein concentration dynamic range. In total, 11 proteins were found as possible markers of diagnosis for bladder cancer. Four of these candidates (afamin, alpha 1-B-glycoprotein, apolipoprotein-A1 and haptoglobin) were previously reported to be urinary markers of bladder cancer. CRP was overexpressed when plasma samples from patients with low grade-Ta tumours were compared to every other sample and may be used as a diagnostic marker. Similarly, afamin and haptoglobin were overexpressed in plasma samples from patients with high grade-high stage tumours when compared to samples from patients with high grade-low stage disease.
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Liu, Yan. "The roles of two different pathways in hypoxia : p53/HDM2 and PERK/GCN2/elF2[alpha] /." View abstract, 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pqdiss&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft_dat=xri:pqdiss:3372356.
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Oliveira, Junior Manoel Carneiro de. "Papel da sinalização purinérgica nos efeitos anti-inflamatórios do treinamento aeróbio em um modelo de asma induzido por ácaro de poeira doméstica." Universidade Nove de Julho, 2017. http://bibliotecatede.uninove.br/handle/tede/1839.
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Asthma is a chronic inflammatory airway disease, in which purinergic signaling has a central role, controlling activation of structural and hematopoietic cells. Aerobic exercise (AE) present anti-inflammatory effects to the airways, but no cellular and molecular mechanisms are known. Thus, this project evaluated the effects of AE on purinergic signaling response in a model of asthma induced by house dust mite (HDM). AE was performed in a treadmill at moderate intensity, 5x/week, during 4 weeks, after 3 weeks of prior HDM administration. HDM (dermatophagoides pteronyssinus; 100mg/mouse) was administered 3x/week, during 7 weeks. The results demonstrated that AE reduced adenosine triphosphate (ATP) accumulation (p<0.001), IL-1beta, IL-4, IL-5, CXCL1/KC, IL-13, IL-17, IL-23, IL-33 and TNF-alpha (p<0.001), while increased IL-1ra, IL-2 and IL-10 in bronchoalveolar lavage (BAL). Total number of leukocytes, eosinophils, lymphocytes and neutrophils in BAL and the number of eosinophils, neutrophils and lymphocytes in the airway wall (p<0.01) were reduced by AE. Airway collagen, elastin, smooth muscle and mucus were reduced by AE (p<0.01). TGF-beta, IGF-1 and VEGF levels was reduced by AE (p<0.001). Lung mechanics (Resistance, Elastance, GTIS, HTIS, RAW) and airway hyperresponsiveness (AHR) to methacholine was ameliorated by AE (p<0.01). IL-4, IL-5 and IL-13 production by lymph nodes, splenocytes and bone marrow cells was also reduced by AE. The expression of P2X7, P2Y2 and P2Y6 by peribronchial leukocytes (p<0.01) and by airway epithelial cells (p<0.01) were reduced by AE. AE reduces asthma phenotype by inhibiting purinergic signaling in a model of HDM-induce asthma.
A asma é uma doença inflamatória crônica das vias aéreas, na qual a sinalização purinérgica tem um papel central, controlando a ativação de células estruturais e hematopoiéticas. O treinamento físico aeróbio (TFA) apresenta efeitos anti-inflamatórios nas vias aéreas, mas não são conhecidos mecanismos celulares e moleculares. Assim, este projeto avaliou os efeitos da TFA sobre a resposta de sinalização purinérgica em um modelo de asma induzida pelo ácaro da poeira doméstica (HDM). TFA foi realizada numa esteira em intensidade moderada, 5x / semana, durante 4 semanas, após 3 semanas de administração anterior de HDM. Foi administrado HDM (dermatophagoides pteronyssinus, 100 mg / camundongo) 3x / semana, durante 7 semanas. Os resultados demonstraram que TFA reduziu a acumulação de adenosina trifosfato (ATP) (p <0,001), IL-1beta, IL-4, IL-5, CXCL1 / KC, IL-13, IL-17, IL-23, IL-33 e TNF-alfa (p <0,001), enquanto aumentou IL-1ra, IL-2 e IL-10 no lavado broncoalveolar (LBA). O número total de leucócitos, eosinófilos, linfócitos e neutrófilos na LBA e o número de eosinófilos, neutrófilos e linfócitos na parede das vias aéreas (p <0,01) foram reduzidos por EA. Colágeno da via aérea, elastina, músculo liso e muco foram reduzidos por EA (p <0,01). Os níveis de TGF-beta, IGF-1 e VEGF foram reduzidos pelo EA (p <0,001). A mecânica pulmonar (resistência, elastância, GTIS, HTIS, RAW) e a hiperreatividade das vias aéreas (AHR) à metacolina foi melhorada pelo EA (p <0,01). A produção de IL-4, IL-5 e IL-13 por nódulos linfáticos, esplenócitos e células da medula óssea também foi reduzida por EA. A expressão de P2X7, P2Y2 e P2Y6 por leucócitos peribronquiais (p <0,01) e por células epiteliais das vias aéreas (p <0,01) foram reduzidas pelo TFA. TFA reduziu o fenótipo de asma inibindo a sinalização purinérgica num modelo de HDM induzindo asma.
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Johansson, Karin. "Planeringshjälpmedel för ökad tillgänglighet på grusvägnätet : en studie av datorverktyget HDM-4 /." Luleå : Luleå tekniska universitet, 2005. http://epubl.luth.se/1402-1757/2005/042.
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Johansson, Karin. "Planeringshjälpmedel för ökad tillgänglighet på grusvägnätet : en studie av datorverktyget HDM-4." Licentiate thesis, Luleå tekniska universitet, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-17550.
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I dagsläget saknas i Sverige ett verktyg som behandlar både belagda och icke-belagda vägar i arbetet med långtidsplanering av vägnätet. För att kunna göra en riktig fördelning av resurser för drift- och underhållsåtgärder över vägnätet krävs ett verktyg som kan behandla samtliga vägtyper och som ger jämförbara resultat. Genom att använda programmet "Highway Development and Management systems", HDM-4, är det möjligt att simulera nedbrytning och åtgärdseffekter för det totala vägnätet, som en enhet. Målet med detta är att kunna simulera vägars tillståndsutveckling och lönsamhet vid olika underhållsstrategier, oavsett beläggningstyp. Kan detta uppnås är förhoppningen att man utifrån en begränsad budget kan utföra det lämpligaste underhållet för att öka standarden på vägnätet. Det faktum att grusvägsmodulen i HDM-4 inte nyttjas i områden med kallt klimat, samt att nedbrytnings- och åtgärdsmodellerna är utvecklade utifrån studier gjorda i områden med totalt annat klimat än Sverige gör att dessa bör valideras innan programmet tas i bruk. Syftet med detta arbete är att genom simuleringar av tillståndsutvecklingen för ett antal grusvägar visa på brister, användningsområden och möjliga idéer till förbättringar av HDM-4. Studier av nedbrytnings- och åtgärdsmodellerna visar på brister i främst anpassningen av modellerna till vinterklimat. I simuleringar med HDM-4 antas ojämnhetsutveckling och materialförlusten från en grusväg vara lika stor över hela året. Detta kan vara rimligt i områden där vägarna inte är snötäckta och frusna stora delar av året. Detta är dock fallet i norra Sverige. Det faktum att nedbrytningen av en väg inte sker på samma sätt under vinterhalvåret som under barmarksperioden tas det heller ingen hänsyn till i modellerna. Simuleringarna visar på möjligheter att använda HDM-4 för att se trender i ojämnhetsutveckling och materialtransport för olika åtgärdsstrategier. De faktorer i modellerna som tar hänsyn till klimat går inte enkelt att anpassa till nordiska förhållanden. Detta medför att tillförlitligheten av de numeriska värdena från simuleringarna bör behandlas med försiktighet när programmet används. Försök med att endast simulera barmarksperioden har även utförts. Dessa visar på en möjlig lösning till att enbart simulera nedbrytningen och effekterna av åtgärder under barmarksperioden. Utifrån de studier av programmet som hittills har utförts finns en möjlighet att kunna använda programmet för prioritering av insatser för det svenska grusvägnätet på en översiktlig nivå. Detta utan att ändra i de ekvationer som ligger till grund för nedbrytningsmodellerna. Programmet är däremot för grovt för att använda vid studier av enstaka objekt i detalj. De variationer i IRI som återspeglar olika nivåer av åtgärder kan tydligt identifieras, vilket innebär att programmet kan användas för att urskilja trender i ojämnhetsutvecklingen för olika åtgärdsalternativ. Ytterligare studier bör utföras för att testa de idéer till ändringar som hittills kommit fram i projektet.Godkänd; 2005; 20061218 (haneit)
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Dias, Chrisanne Silvia. "Role of Stress-Induced Alternative Splicing of HDM2 in Human Tumor and Non-Tumorigenic Cell Lines." Ohio University / OhioLINK, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1164990015.
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Skorinko, Jeremy Kenneth. "Regional Mechanical Function Changes Remain after Ventricular Pacing Cessation: Evidence of Mechanical Cardiac Memory." Digital WPI, 2010. https://digitalcommons.wpi.edu/etd-theses/183.
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Every year 400,000 - 600,000 people in the United States die from sudden cardiac death. Sudden cardiac death is often caused by irregular electrical impulses, or arrhythmias, in the heart. Arrhythmias can be corrected through pharmacological therapies, device therapies, or both. One type of device therapy, pacemakers, are inserted in the heart to correct arrhythmias. After a period of ventricular pacing, cardiac memory is defined by changes in the T-wave that are persistent upon return to normal activation pathways. During ventricular pacing, regional stroke work in areas closest to the pacing electrode is significantly decreased. We hypothesize that the mechanical function in the region around the pacing site will continue to have altered mechanical function after cession of pacing, in effect showing a mechanical cardiac memory. To test the hypothesis, nine canine models were implanted with pacing electrodes in both the atrium and ventricle. After a forty- minute stabilization period, baseline data were obtained during atrial pacing. Cardiac memory was induced in five canine models through a two-hour period of ventricular pacing followed immediately by atrial pacing. The remaining canine models served as controls, undergoing atrial pacing for two hours. High- density mapper (HDM) was used to determine mechanical function in a region centered approximately 1 cm away from the pacing electrode. No differences in global function (tau, developed pressure, dP/dtmax, dP/dtmin) were found after two hours of ventricular pacing upon return to normal activation pathways. There was a significant decrease in regional stroke work in an area close to the electrode between baseline (5.7 ± 2.6 %), during ventricular pacing (-3.8 ± 0.9 %)(p<0.05) and after two hours of ventricular pacing upon return to normal activation pathways (2.4 ± 1.6 %)(p<0.05). Further, systolic area contraction was also significantly different between baseline (5.0 ± 6.6 %) and after two hours of ventricular pacing upon return to normal activation pathways (0.2 ± 7.4 %)(p<0.05). Diastolic twist and diastolic twist rates showed no significant differences. Finally, contractile principal strain increased by inducing cardiac memory (-2.6 ± 0.3 %) as compared to baseline (-1.1 ± 0.5 %)(p<0.05). These findings suggest there is a mechanical correlation to electrical cardiac memory.
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Gama, Vivian. "Regulation of the Anti-apoptotic Protein Ku70 and the Implications for Bax-Mediated Apoptosis." Case Western Reserve University School of Graduate Studies / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=case1221831227.
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Foroughi, pour Ali. "Linear Approximations for Second Order High Dimensional Model Representation of the Log Likelihood Ratio." The Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1555419601408423.
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Jonsson, Hanna. "Allergenspecifik immunterapi - Effekter hos barn med HDM-inducerad astma med eller utan rinit." Thesis, Linnéuniversitetet, Institutionen för kemi och biomedicin (KOB), 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-64918.
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Allergiska sjukdomar är kroniska tillstånd som tycks öka globalt och är vanligt förekommande i unga åldrar. Allergiska individer visar överkänslighet mot ofarliga allergiframkallande ämnen, allergener. Överkänslighetsreaktioner mot husdammskvalster -allergen (HDM) är en IgE-medierad reaktion och tycks vanligen förekomma i varma temperaturer med hög luftfuktighet. Utöver de vanliga behandlingsterapier som kan lindra de typiska allergiska symtomen: astma och rinit finns en behandling som kan modifiera immunsystemets överkänslighetsreaktioner, allergenspecifik immunterapi (ASIT). ASIT administreras subkutant eller sublingualt med upprepade doser av de specifika allergenerna som patienten visar överkänslighet mot och ökar därmed immuntolerans. Syftet med detta litteraturarbete var att undersöka ASITs effekter avseende symtomlindring, medicinering behov och lungfunktioner mätt med PEF och FEV1 hos barn med HDM-inducerad astma med eller utan rinit. Fem kliniska kontrollerande studier valdes från databasen PubMed och analyserades för att besvara arbetets syften. ASIT kontrollerades inte med placebogrupper i någon av de valda studierna, istället kontrollerades ASIT med enbart standardiserade läkemedelsbehandling av inhalerade glukokortikoider (ICS). Förbättrade symtompoäng för astma samt reducerade dygnsdoser av ICS uppnåddes med ASIT i studierna 1, 4 och 5. God astmakontroll bedömdes i studie 2 och 3 med signifikant reducerade ICS-doser hos deltagare behandlade med ASIT. I studie 1 bedömdes 13 deltagare (28,9%) med ASIT kunna avsluta behandlingen av ICS för att erhålla god astmakontroll liksom nio deltagare (20,0%) som endast fick läkemedelsbehandling. I studie 2 bedömdes 52,4 % av deltagarna som behandlades med ASIT kunna avsluta behandlingen med ICS och liksom 9,1 % av deltagarna i kontrollgruppen. Förbättrade astmasymtom uppnåddes med ASIT enligt signifikant reducerad 10 cm VAS-skala efter tre år i studie 4. Deltagarnas lungfunktioner förbättrades med ASIT i studie 1, 2, 3 och 5 avseende signifikant ökade PEF- eller FEV1-värden. Studie 4 redovisade en marginell förbättring av deltagarnas lungfunktioner med mätt FEV1 men visade ingen signifikant skillnad från start till efter tre års behandling med ASIT. Studie 4 och 5 som var de enda studierna i detta arbete som undersökte ASIT’s effekter på HDM-inducerad rinit visade signifikant reducera symtompoäng för rinit. De valda studiernas redovisade resultat stärks vid den översiktliga utvärdering då behandlingarna utgjordes på en specifik målgrupp på lika indikationer under lång tid. ASIT uppnår god symtomlindring, minskat medicineringsbehov samt förbättrade lungfunktion hos barn med mild till måttlig HDM-inducerad astma. För att öka övervägandet av ASIT som ett behandlingsalternativ till barn med HDM-inducerad astma och rinit bör fler större kontrollerade studier genomföras. Vidare undersökningar kan tänkas vara studier som jämför administreringsmetod av ASIT hos barn samt om ASIT kan motverka barns framtida överkänslighetsreaktioner.The prevalence of allergic diseases seems to increase globally and is common in childhood. Allergic individuals show hypersensitivity to harmless allergy-causing substances, allergens. Hypersensitivity to house dust mite is an IgE-mediated reaction that usually occurs in warm climates with high humidity. Common symptoms of allergy to house dust mites includes asthma and rhinitis. Treatment with allergen-specific immunotherapy can modify the immune system and thereby increase immune tolerance. ASIT (allergen-specific immunotherapy) is given in repeated doses and can be administrered subcutaneously or by sublingually drops and tablets. The purpose of this literature review was to investigate the effects of ASIT on symptomatic relief, use of medication needs and lung functions measured with PEF and FEV1 in children with HDM-induced asthma with or without rhinits. Five clinical randomized controlled studies available in full text were selected from the PubMed database and analyzed to answer the purpose of this work. All studies included children allergic to house dust mite-allergic and diagnosed with asthma. Studies 4 and 5 also included children with HDM induced rhinits. ASIT was not controlled whit placebo treatment in any of the selected studies. ASIT was controlled by standardized pharmacotherapy included inhaled glucocorticoids (ICS).Improved symptom points for asthma and reduced daily doses of ICS were achieved with ASIT in studies 1, 4 and 5 (P <0,05). Asthma control was assessed in studies 2 and 3 with significantly reduced ICS doses in patients treated with ASIT (P <0,05). In study 1, 13 participants (28,9 %) with ASIT were assessed to complete treatment of ICS to provide good asthma control, and similar results provide 9 participants (20%) who only received pharmacotherapy. Improved asthma symptoms were achieved with ASIT according to reduced 10 cm visual analog scale (VAS) after three years in study 4. In studies 1, 2, 3 and 5 childrens lungfunctions improved with ASIT regarding significantly increased PEF and FEV1 values (P<0,05). Study 4 presented a marginal improvement in participants lungfunctions but showed no significant difference from start to after three years of treatment with ASIT. ASIT in studies 4 and 5 showed significantly reducing symptom scores for rhinitis. The treatments consisted of a specific target group on equally indications during a long period of time. ASIT achieves good symptom relief, reduced medication needs, and improved lungfunctions in children with mild to moderate HDM induced asthma. Further large clinical controlled studies are needed, to evaluate ASIT as an alternative treatment of allergic reactions in children to house dust mit. Further studies that may be of interest would be comparing ASIT’s administration for children regarding safety and ASIT’s effect of being able to counteract new hypersensitivity reactions.
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Shetty, Manish. "Catalytic upgrading of biomass through the hydrodeoxygenation (HDO) of bio-oil derived model compounds." Thesis, Massachusetts Institute of Technology, 2017. http://hdl.handle.net/1721.1/114309.
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Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemical Engineering, 2017.Cataloged from PDF version of thesis.
Includes bibliographical references.
Lignocellulosic biomass is an attractive renewable source for fuels and chemicals. Of the many conversion alternatives, catalytic fast pyrolysis has emerged as an attractive technology to convert biomass into fuel additives and value-added chemicals. Current pyrolysis oils or bio-oils are incompatible with refinery streams due to their high acid, water, and water content. The key roadblock in its commercial exploitation is development of catalytic platforms for selective deoxygenation along with minimum hydrogen consumption and carbon loss. Current catalytic solutions including zeolites, and conventional hydrotreating catalysts employ high hydrogen pressures, leading to aromatic ring hydrogenation, and hydrogen consumption. This thesis focusses on developing fundamental catalytic understanding on cheaper and earth-abundant reducible transition metal oxide catalysts for selective hydrodeoxygenation (HDO) of bio-oil derived model compounds using reactivity, computation and characterization studies. The first section focuses on developing structure-reactivity relationships on bulk and supported MoO₃ catalysts for the HDO of lignin-derived model compounds. Characterization reveals that MoO₃ undergoes reduction to catalytically inactive MoO₂ at a temperature of 673 K, and stabilization of partially reduced MoO₃ surface through its partial carburization to oxycarbide phase (MoOxCyHz) at temperatures < 623 K. Thereafter, TiO₂ and ZrO₂ supports prevent the reduction of dispersed oligomeric MoOx species to catalytically inactive species, enhancing their stability. In addition, the overall catalyst reactivity inversely correlates to the maximum hydrogen consumption temperature during hydrogen temperature programmed reduction (H₂-TPR). Furthermore, a near-monolayer oligomeric MoOx dispersion on ZrO₂ support was found to be optimum for HDO reactivity. The second section focuses on developing mechanistic insights into the HDO on bulk and supported MoO₃ with the aid of density functional theory (DFT) computations and kinetic studies. DFT computations were carried out on the elementary steps for HDO of acetone-a model compound on pristine [alpha]-MoO₃ (010) surface to reveal dissociative H₂ adsorption on the (010) surface to be the rate-limiting step. Kinetic studies on MoO₃ supported on ZrO₂ reveal the differences in reaction mechanism and the nature of active sites for HDO on MoO₃/ZrO₂ as compared to bulk MoO₃. The third section focuses on generalizing the low-temperature (< 523 K) selective HDO on other reducible base metal oxides, specifically cobalt oxide and demonstrates oxides to have significantly higher reactivity than base metals for HDO. Finally, lanthanum strontium cobaltite (La₀.₈Sr₀.₂CoO₃), a perovskite oxide, was demonstrated as a novel HDO catalyst at < 523 K. Overall, this thesis provides a toolkit for developing structure-reactivity relationships on reducible metal oxides for their use as HDO catalysts.
by Manish Shetty.
Ph. D.
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Bertseva, Elena. "ICLAS-VeCSEL near 1 micronM : spectral condensation and molecular spectroscopy (HDO, C2H2, C2D2, N2O)." Université Joseph Fourier (Grenoble), 2003. http://www.theses.fr/2003GRE10090.
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Lerch, Rafael Lucca. "Previsão de irregularidade pós-recape em rodovias do RS : ajuste no modelo HDM-4." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2002. http://hdl.handle.net/10183/2981.
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Esta dissertação analisa a evolução da irregularidade longitudinal de pavimentos, devida à execução de recapes em concreto asfáltico. Consideraram-se nove trechos de rodovias, totalizando aproximadamente 265 km, com estruturas representativas da malha viária do Estado do Rio Grande do Sul. Registraram-se irregularidades antes e após o recape, e as reduções medidas foram comparadas com valores previstos pelos modelos linear e bilinear propostos pelo programa HDM-4 do Banco Mundial. As diferenças entre reduções previstas e medidas alertaram para a necessidade de ajuste dos modelos em alguns casos, levando em conta as estruturas dos pavimentos e as espessuras de recape analisadas. Propõe-se nesta dissertação valores para os parâmetros ajustáveis dos modelos, com cuja aplicação foram minimizadas as diferenças entre as reduções medidas e previstas. De forma complementar, desenvolveu-se um estudo de sensibilidade para determinar o efeito que as diferenças, por ventura existentes, entre espessuras projetadas e executadas de recape poderiam ter na redução da irregularidade. Também, por meio de uma análise estatística, obteve-se um modelo para previsão da redução da irregularidade em função da espessura do recape e da irregularidade anterior à execução do mesmo.
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Schonhoff, Christopher M. "The Regulation of nNOS During Neuronal Differentiation and the Effect of Nitric Oxide on Hdm2-p53 Binding: a Dissertation." eScholarship@UMMS, 2000. https://escholarship.umassmed.edu/gsbs_diss/57.
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Nitric oxide is a ubiquitous signaling molecule with both physiological and pathological functions in biological systems. Formed by the enzymatic conversion of arginine to citrulline, NO, has known roles in circulatory, immune and nervous tissues. In the nervous system nitric oxide has been implicated in long-term potentiation, neurotransmitter release, channel function, neuronal protection and neuronal degeneration. Much of our work has focused on yet another role for nitric oxide in cells, namely, neuronal differentiation.
During development, neuronal differentiation is closely coupled with cessation of proliferation. We use nerve growth factor (NGF)-induced differentiation of PC12 pheochromocytoma cells as a model and find a novel signal transduction pathway that blocks cell proliferation. Treatment of PC12 cells with NGF leads to induction of nitric oxide synthase (NOS). The resulting nitric oxide (NO) acts as a second messenger, activating the p21(WAF1) promoter and inducing expression of p21(WAF1) cyclin-dependent kinase inhibitor. NO activates the p21(WAF1) promoter by p53-dependent and p53-independent mechanisms. Blocking production of NO with an inhibitor of NOS reduces accumulation of p53, activation of the p21(WAF1) promoter, expression of neuronal markers, and neurite extension. To deternine whether p21(WAF1) is required for neurite extension, we prepared a PC12 line with an inducible p21(WAF1) expression vector. Blocking NOS with an inhibitor decreases neurite extension, but induction of p21(WAF1) with isopropyl-1-thio-beta-D-galactopyranoside restored this response. Levels of p21(WAF1) induced by isopropyl-1-thio-beta-D-galactopyranoside were similar to those induced by NGF. Therefore, we have identified a signal transduction pathway that is activated by NGF; proceeds through NOS, p53 and p21(WAF1) to block cell proliferation; and is required for neuronal differentiation by PC12 cells.
In further studies of this pathway, we have examined the role of MAP kinase pathways in neuronal nitric oxide synthase (nNOS) induction during the differentiation of PC12 cells. In NGF-treated PC12 cells, we find that nNOS is induced at RNA and protein levels, resulting in increased NOS activity. We note that neither nNOS mRNA, nNOS protein nor NOS activity is induced by NGF treatment in cells that have been infected with a dominant negative Ras adenovirus. We have also used drugs that block MAP kinase pathways and assessed their ability to inhibit nNOS induction. Even though U0126 and PD98059 are both MEK inhibitors, we find that U0126, but not PD98059, blocks nNOS induction and NOS activity in NGF-treated PC12 cells. Also, the p38 kinase inhibitor, SB 203580, does not block nNOS induction in our clone of PC12 cells. Since the JNK pathway is not activated in NGF-treated PC12 cells, we determine that the Ras-ERK pathway and not the p38 or JNK pathway is required for nNOS induction in NGF-treated PC12 cells. We find that U0l26 is much more effective than PD98059 in blocking the Ras-ERK pathway, thereby explaining the discrepancy in nNOS inhibition. We conclude that the Ras-ERK pathway is required for nNOS induction.
The activation of soluble guanylate cyclase and the production of cyclic GMP is one of the best characterized modes of NO action. Having shown that inhibition of NOS blocks PC12 cell differentiation we tested whether nitric oxide acts through soluble guanylate cyclase to lead to cell cycle arrest and neuronal differentiation. Unlike NOS inhibition, the inhibition of soluble guanylate cylcase does not block the induction of neuronal markers. Moreover, treatment of NGF-treated, NOS-inhibited PC12 cells with a soluble analog of cyclic GMP was unable to restore differentiation of those cells. Hence, cGMP is not a component of this pathway and we had to consider other mechanisms of NO action.
It has become increasingly evident that another manner by which NO may exert its effects is by S-nitrosylation of cysteine residues. We tested, in vitro whether nitric oxide may control p53 by S-nitrosylation and inactivation of the p53 negative regulator, Hdm2. Treatment of Hdm2 with a nitric oxide donor inhibits Hdm2-p53 binding, the first step in Hdm2 regulation of p53. The presence of cysteine or DTT blocks this inhibition of binding. Moreover, nitric oxide inhibition of Hdm2-p53 binding was found to be reversible. Sulfhydryl-sensitivity and reversibility are consistent with nitrosylation. Finally, we have identified a critical cysteine residue that nitric oxide modifies in order to disrupt Hdm2-p53 binding. Mutation of this residue from a cysteine to an alanine does not interfere with binding but rather eliminates the sensitivity of Hdm2 to nitric oxide inactivation.
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Sestini, Valéria Maria. "Uma adaptação dos modelos de deterioração do HDM III à região centro de São Paulo." Universidade de São Paulo, 1997. http://www.teses.usp.br/teses/disponiveis/18/18137/tde-28022018-143727/.
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Com o objetivo de comparar as condições do pavimento previstas pelo modelo HDM III com as observadas, foram avaliados três trechos rodoviários, divididos num total de 19 subtrechos homogêneos, na região central do estado de São Paulo. O método adotado para medir a irregularidade do pavimento nestes trechos foi o de nível e mira. As deflexões recuperáveis foram medidas com a viga Benkelman e com aparelho tipo FWD (\"Falling Weight Deflectometer\"). O levantamento da condição da superfície baseou-se na especificação de serviço DNER-ES 128/83 e no procedimento DNER-PRO 08-94. Durante todo o período de avaliação foram feitos registros em vídeo (filmagens), dos trechos selecionados. Foi feita uma contagem de tráfego em cada trecho e definido o fator de veículo com base em dados de pesagem de 1993. Com base nestes dados foram testados alguns dos modelos de deterioração do HDM III, aplicados aos casos específicos dos pavimentos que compõem os trechos avaliados. Verificada a necessidade de adaptação destes modelos à região de estudo foram definidos, através de análise de regressão, alguns fatores de deterioração para serem aplicados diretamente no modelo HDM III. Tendo em vista que o modelo HDM III utiliza a deflexão medida com a viga Benkelman, definiu-se uma correlação destes resultados com a deflexão recuperável do pavimento medida com equipamentos tipo FWD.Three highway section divided in nineteen homogeneous subsections were studied in order to compare the pavement conditions predicted by the HDM III model with the values measured \"in situ\". The roughness was measured by the road and level method and recoverable deflections with Benkelman beam and FWD (Falling Weight Deflectometer). The surface distress survey was based on DNER-ES 128/83 and DNER-PRO 08/94 standards. Television tapes were recorded during the period of evaluation. Traffic countings in each section were rnade and the equivalent standard axle load factors were stabilished, based on load data from 1993. Running the HDM III software with these data, the prediction models within the HDM III were tested. For the sections under analysis it had shown that adaptations to the deterioration factors should be done. These new deterioration factors are suggested. Since the HDM III model uses the Benkelman beam values, a correlation between FWD and Benkelman beam was established.
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Chua, Kui Hong, and kuihongchua@hotmail com. "Studies on Andrographis paniculata (Burm. f.) Nees (HDM 15) A Medicinal Native Plant of Brunei Darussalam." RMIT University. Health Sciences, 2007. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20080703.112512.
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Ethno botanical surveys have revealed that Brunei Darussalam has a rich source of tropical medicinal plants. As 80% of the country's land is covered by tropical rainforest, Brunei Darussalam may have some medicinal plants with unique characteristics of secondary metabolites. Some plants such as Catharanthus roseus (L.) G. Don and Eurycoma longifolia Jack have long been used by the local communities to treat various disease conditions. However, no research has been done in terms of the constituents or biological activities of the Brunei Darussalam medicinal plants. We have investigated the genetic variability diversity and pharmacological actions of Andrographis paniculata (Burm.f.) Nees [1] also known as Daun Pahit or Chuan Xin Lian or King of Bitters by an interdisciplinary approach, involving DNA-based RAPD and RFLP analyses, HPLC-based chemical analysis as well as cell culture and tissue-based bioassays. We have demonstrated that Andrographis paniculata extr acts exhibited a range of actions including antioxidant, anti-allergies, anti-inflammatory and anti-cancer effects. Some of the pharmacological actions of Andrographis paniculata are co-related with their active constituents Andrographolide (A) and Dehydroandrographolide (D). The study is valued not only in obtaining experimental evidence for supporting traditional use of native medicinal plants but also in establishing a platform for studying other medicinal plants in Brunei Darussalam.
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Mrawira, Mrawira Donath Massawe. "Streamlining the World Bank's Highway Design and Maintenance Standards model (HDM-III) for network-level application." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq21372.pdf.
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Klein, Frederico de Carvalho. "Análise da influência de características geométricas de rodovias nos custos dos usuários utilizando o programa HDM-4." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/18/18137/tde-11122006-161658/.
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A dissertação tem como objetivo verificar e analisar a influência das características geométricas de trechos de rodovia no custo total dos usuários, em termos de custo de operação dos veículos e custo do tempo de viagem, com a utilização do programa computacional HDM-4 (Highway Development and Management). Para esta análise, foi realizado um estudo de caso para o qual foram criados sete trechos representativos das classes de geometria consideradas pelo HDM-4 e dezenove trechos com características geométricas que permitissem uma análise de sensibilidade dos parâmetros relacionados às curvaturas vertical e horizontal das vias. Foram selecionados, ainda, quatro trechos da rodovia Washington Luiz (SP-310), representativos de rodovias com distintas características geométricas. A simulação foi realizada fixando-se um cenário de fatores que compõem o modelo HDM-4: tipo de fluxo de tráfego, classe da rodovia, composição e volume de tráfego, tipo e condição do pavimento, dentre outros. O estudo de caso permitiu a quantificação dos efeitos da geometria sobre todas as parcelas que compõem os custos dos usuários. Foram calculados os custos de terraplenagem e pavimentação para diferentes curvaturas verticais com a finalidade de comparação dos custos de melhorias no projeto de rodovias existentes com os benefícios decorrentes dessas intervenções. Os resultados obtidos mostraram que a geometria pode ter grande influência nos custos totais dos usuários e, portanto, seus efeitos devem ser considerados em projetos de construção e de manutenção de rodovias, possibilitando maiores benefícios à sociedade.The main goal of the master dissertation is to verify and to analyze the influence of geometric characteristics of road sections on highway users costs, in terms of vehicle operation costs and costs associated to travel time, using the software HDM-4 (Highway Development and Management). The study was initiated with the creation of seven sections representing the HDM-4 highway geometric classes, and nineteen sections with geometric characteristics defined to allow a sensitivity analysis of parameters associated to vertical and horizontal alignments. Four sections were selected from Washington Luiz highway (SP-310), representing highways with distinct geometric characteristics. The computer simulations were performed considering a scenario that includes: traffic flow pattern, road class, average daily traffic and traffic composition, pavement type and condition, among others. The study calculated the effects of highway geometry on each component of users costs. The costs of earth work and paving were calculated aiming at the comparison between costs of geometric improvements and benefits associated to that. The results showed that highway geometry may have a great influence on users costs and, therefore, its effects must be considered in highway construction and maintenance projects assuring more benefits to society.
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Lluncor, Yataco Gianmarco Xavier. "Aplicación del Modelo HDM en la Evaluacion de Proyectos de Carreteras en Peru: "Carreteras Bagua Chica-Flor de la Esperanza"." Bachelor's thesis, Universidad Ricardo Palma, 2012. http://cybertesis.urp.edu.pe/handle/urp/445.
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1.1 Tema
Aplicación del Modelo HDM III en la evaluación de proyectos de Carreteras en el Perú: Carretera “Bagua Chica – Flor De La Esperanza ubicada en el departamento de Amazonas”.
1.2 Marco Situacional
El presente estudio tiene por objetivo analizar la aplicabilidad del Modelo HDM III en carreteras en Perú revisando el caso del estudio de la mejor alternativa de construcción de la carretera “Bagua Chica – Flor de la Esperanza”, ya sea siguiendo una primera alternativa que es trazo del proyecto de ingeniería de detalle o la segunda alternativa que es el trazo de la variante propuesta, para la cual se evaluarán los costos de operación de los vehículos al circular por una u otra alternativa.
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Becker, Virgínia Esther Gueller. "Aplicação do modelo de Tavakoli para gerência de manutenção de pavimentos em cidade de médio porte." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/3/3138/tde-18062013-155806/.
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Este trabalho foi realizado com o objetivo de aplicar o Modelo de Tavakoli, que é um modelo de gerência de pavimentos para vias urbanas, para análise de priorização de vias urbanas em cidade de médio porte. Para isso se utilizou um estudo de campo previamente realizado pela Prefeitura Municipal de Suzano e pelo Laboratório de Mecânica de Pavimentos da Universidade de São Paulo no município de Suzano, no ano de 2007, com o qual foi criado um banco de dados das vias do município. Naquele estudo foi empregado o modelo HDM-4 do Banco Mundial para a priorização de intervenções de manutenção nas vias públicas. No presente trabalho buscou-se elaborar um estudo comparativo entre aquela solução e a solução atingida pelo modelo de Tavakoli para um mesmo conjunto de vias. Para este trabalho o Modelo de Tavakoli foi adaptado para planilhas eletrônicas e aplicado em um conjunto de seções de pavimentos, permitindo a determinação de uma lista de prioridades de intervenções. Posteriormente, essas intervenções foram simuladas para o mesmo conjunto de vias, buscando a priorização dos serviços, com o modelo HDM-4 versão 1.0. Através deste trabalho foi possível concluir que os dois critérios estudados podem ser úteis para gerenciar vias urbanas, pois priorizaram as intervenções necessárias nas vias urbanas analisadas. Foi possível verificar que o Modelo de Tavakoli se revelou mais conservador, por ser um critério fechado, com um índice de prioridades dependendo diretamente do índice da condição do pavimento e do volume diário médio de tráfego, afetando diretamente a classificação das seções. No Modelo HDM-4 a classificação das seções e os valores de irregularidade não tiveram uma sincronia, pois o HDM-4 é um modelo de critérios abertos, que permite ao usuário acrescentar vários parâmetros, e com estes foi possível reproduzir uma situação um pouco mais próxima da realidade.This study was conceived in order to apply the Tavakoli Model, which is a pavement management system for urban roads, aiming the analysis of prioritization of urban streets in a mid-sized city. A field study previously conducted by Suzano City and the Pavements Mechanics Laboratory of the University of São Paulo in Suzano City in 2007 was used, from which was created a database of the towns streets. In that study, the Highway Design and Management (HDM-4) Model of the World Bank was employed for prioritizing maintenance work on public roads. In this study it was sought to develop a comparative study between the mentioned solution and the one reached by Tavakolis Model in the same set of roads. In this research the Tavakoli Model was adapted for the spreadsheets and applied to a set of pavement sections, allowing the determination of a priority list of interventions including the specific type of intervention for each segment. Subsequently, these same types of strategies or interventions were simulated for the same set of paths, seeking the prioritization, with HDM-4 Model version 1.0. Through this research it was possible to conclude that both criteria studied can be useful for managing urban roads, because they prioritized the necessary interventions in the urban roads analyzed. The Tavakoli Model proved to be more conservative, on account of being a closed criterion, with a priority index depending directly on the pavement condition index and average daily volume of traffic, affecting the classification of the sections directly as well. In the HDM-4 Model, the classification of the sections and values of the International Roughness Index did not match, due to HDM-4 being an open criteria model, which allows the user to add several parameters, and they can lead to more realistic report.
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Alexandrov, Vadim. "An ab-initio study of vibration dynamics in hydrogen-bonded compounds and intramolecular energy flow in the HDO molecule." Diss., The University of Arizona, 2001. http://hdl.handle.net/10150/289722.
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Several new techniques were developed and applied to investigate various non-trivial aspects of vibrational motion. In particular, a novel strategy for accurate determination of vibrational energy levels in the presence of intramolecular hydrogen bonds was proposed. A small subset of convenient internal coordinates was chosen to represent a vibrational mode of interest, while the interdependence among all other coordinates was encoded in the g-matrix elements which appear in the kinetic energy operator. Individual g-matrix elements were modeled by series of shifted Gaussian functions, a newly proposed functional form that assured physically correct behavior for the entire domain of internal coordinates. The success in reproducing experimentally observed frequencies should be partly attributed to a new basis set of modified Hermite-type functions which has been introduced in this work and employed in all presented vibrational energy calculations. A novel functional representation for potential energy surfaces (PES) was also proposed in this work. The new representation, consisting of products of shifted Gaussians and shifted Morse functions, reproduces all local features near the bottom of the well and also possesses correct asympototic behavior. Determination of the functional parameters for the PES fits involved a simultaneous optimization of many linear and nonlinear parameters, and required a development of an efficient minimization routine. The new routine consisted of an iterative procedure, which treated sets of linear and nonlinear parameters separately and in successive order in each iteration. The above-mentioned set of developed tools was applied to study the intramolecular vibrational energy redistribution (IVR) in HDO molecule. The time-evolution of the O-H stretching local mode was studied by a time-propagation method based on matrix representation of the evolution operator. The energy transfer among the local modes was modeled by non-diagonal g-tensor terms in the kinetic energy operator and by three-dimensional potential energy operator. The results indicate that the O-H stretching local modes must be substantially long-lived within the chosen model. A new recursive programming technique combined with a very effective passing by reference methodology was developed and used in this study to implement the time-propagation routine. The introduced concept of a shared compound pointer in Fortran 90 automatically accounts for the changing sizes of all data structures, while working with only a single parameter, a pointer to a parent object.
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Kelly, Melissa Ann Blake Geoffrey A. "Some new measurements of the ground and v₂2=1 : states of HDO in the region 200-750 GHz /." Diss., Pasadena, Calif. : California Institute of Technology, 2003. http://resolver.caltech.edu/CaltechETD:etd-09162002-140330.
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Alekic, Anis, and Andreas Carmvall. "Displayintegrering." Thesis, Linnaeus University, School of Computer Science, Physics and Mathematics, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-2412.
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Arbetet är ett examensarbete i högskoleingenjörsutbildningen, inriktning datateknik, på Linnéuniversitet. Arcoma AB är ett företag i Växjö som utvecklar och tillverkar kompletta röntgensystem. Företaget har som mål att förbättra användarvänligheten av systemet med hjälp av en LED-display. De kan förbättra systemet genom att byta ut en enhet som styr röntgengeneratorn mot LED-displayen. Detta leder till att användaren får bättre kontroll och styrning av systemet, samtidigt som patienten behandlas.
I rapporten beskrivs implementering av både hårdvara och mjukvara till displayen, samt den tekniska bakgrunden som till exempel HDMI och LED-tekniken. Mjukvaran har testats direkt mot displayen vilket har gjort testningen intressant. För att lösa uppgiften har vi fått använda Arcomas system och utvecklingsverktyg. Programspråket som användes var framförallt C++.
Resultatet blev ett system som byggs upp av Arcomas hårdvaruplattform, vår kringelektronik och displayen där de sistnämnda byggdes in i en låda. Utöver displayen utvecklades en demoapplikation för generatorstyrning som styrs av en USB-mus. Arcoma är nöjda över resultatet då vi löst huvuduppgiften med displayen, men även löst de extrauppgifter som fanns. Idag försöker Arcoma skapa en efterfrågan för lösningen i deras nya produkter.
This abstract describes the Bachelor of Science in engineering thesis for computer science at Linnaeus University. Arcoma AB in Växjö is a company that constructs and develops complete x-ray systems. The goal of the company is to improve the usability of the system with the help of a LED display. Arcoma can improve the system by exchange the unit that controls the x-ray generator with the LED display instead. This will give the user the ability to have better overview and control of the system, while treating the patient.
This report describes the implementation of both the software and the hardware for the display, but also the technical background for some standards like HDMI and the LED technologic. The software has been tested directly to the display, and therefore made the testing interesting. We have used the Arcoma system and the development tools to solve the problem. The programming language that was used to solve the problem was mainly C++.
The result was a system that is build up by the Arcoma hardware platform, our surrounding electronics and the display where the last ones were built in a box. Aside the display a demo application was developed for controlling the generator. This applications is controlled by a USB mouse. Arcoma is pleased with the result of the main problem with the display, and they are also pleased with us solving the additional problems that existed. Arcoma wants to create a demand of this solution in their new products.
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Montoya, Goicochea Jorge Eduardo. "Implementación del Sistema de Gestión de Pavimentos con Herramienta HDM-4 para la Red Vial Nro. 5 Tramo Ancón - Huacho - Pativilca." Bachelor's thesis, Universidad Ricardo Palma. Programa Cybertesis PERÚ, 2007. http://cybertesis.urp.edu.pe/urp/2007/montoya_je/html/index-frames.html.
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Bui, Phuong Phuc Nam. "Catalytic Hydrodeoxygenation of Bio-Oil Model Compounds (Ethanol, 2-Methyltetrahydrofuran) over Supported Transition Metal Phosphides." Diss., Virginia Tech, 2013. http://hdl.handle.net/10919/52641.
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The objective of this project is to investigate hydrodeoxygenation (HDO), a crucial step in the treatment of bio-oil, on transition metal phosphide catalysts. The study focuses on reactions of simple oxygenated compounds present in bio-oil -- ethanol and 2-methyltetrahydrofuran (2-MTHF). The findings from this project provide fundamental knowledge towards the hydrodeoxygenation of more complex bio-oil compounds. Ultimately, the knowledge contributes to the design of optimum catalysts for upgrading bio-oil.
A series of transition metal phosphides was prepared and tested; however, the focus was on Ni2P/SiO2. Characterization techniques such as X-ray diffraction (XRD), temperature-programmed reduction and desorption (TPR and TPD), X-ray photoelectron spectroscopy (XPS), and chemisorption were used. In situ Fourier transform infrared (FTIR) spectroscopy was employed to monitor the surface of Ni2P during various experiments such as: CO and pyridine adsorption and transient state of ethanol and 2-MTHF reactions. The use of these techniques allowed for a better understanding of the role of the catalyst during deoxygenation.Ph. D.
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Akula, Kavitha. "Expanding the Spiroligomers Toolbox as Protein-Protein Interaction Inhibitors." Diss., Temple University Libraries, 2017. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/422281.
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ChemistryPh.D.
This work presents the application of spiroligomers as inhibitors of protein-protein interactions. After the discovery of an acyl-transfer coupling reaction by Dr. Zachary Brown, a previous graduate student of Schafmeister group, the synthesis of highly functionalized spiroligomers that mimic the helical domain of p53 was undertaken before each molecule was tested for binding to HDM2, a natural binding partner of p53. A library of molecules was synthesized on solid support that altered the stereochemistry along the spiroligomer as well as the presented functional groups. It was determined that spiroligomers enter human liver cancer cells through passive diffusion and induces a biological response in both a dose- and time-dependent manner. The synthesis of additional spiroligomer analogues achieved low micromolar to high nanomolar range activity during screening in direct and competitive binding assays. In parallel to the project above, a series of spiroligomers that mimic the side chains of the leucine zipper region of Max were synthesized in an effort to disrupt the interaction of the protein with c-Myc. The series of compounds contained various stereocenter combinations and different functional groups as before but were made in solution before testing for inhibition. Initial binding assays resulted in low micromolar activity, however, secondary assays (ELISA and cellular assays) did not confirm the inhibitory effect of spiroligomers on the c-Myc/Max heterodimer. In summary, this work illustrates that spiroligomers are capable mimics of helical peptides and can induce a biological response.
Temple University--Theses
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Serra, Pagès Mariona. "Selective EP2 agonism attenuates hdm-induced murine airway pathology and mast cell activity, and triggers intracellular inhibitory signaling in mast cells." Doctoral thesis, Universitat Autònoma de Barcelona, 2012. http://hdl.handle.net/10803/84009.
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L'asma al lèrgica és una malaltia respiratòria crònica amb una alta prevalença en
els països desenvolupats. Els tractaments actuals no aturen el procés al lèrgic
subjacent ni aconsegueixen el control dels símptomes de la malaltia. El tractament
més eficaç és l'ús de corticoesteroids, que es basen en modificacions químiques de
potents hormones endògenes antiinflamatòries. L'estudi de vies antiinflamatòries
endògenes és una estratègia experimental eficaç per descobrir noves dianes
terapèutiques potencials contra l'asma. Una d'aquestes vies endògenes és la de la
ciclooxigenasa (COX). Prostaglandina (PG) PGE2, un producte de la COX, s'ha
suggerit que exerceixen un efecte protector en els pulmons. En particular, els
estudis experimentals en pacients amb asma van revelar que la PGE2 inhalada
redueix la hiperreactivitat bronquial i la inflamació. Aquest efecte de protecció de la
PGE2 també s'ha demostrat, directament i indirectament, en ratolins sensibilitzats
amb OVA o HDM. Els mecanismes subjacents als efectes beneficiosos de la PGE2 en
l'asma no es coneixen. Una de les característiques més constants de la PGE2 és la
seva capacitat de modular l’activitat dels mastòcits in vitro. Els nostres darrers
estudis in vivo van mostrar que la PGE2 també inhibeix l’activitat dels mastòcits en
ratolins sensibilitzats a HDM i que aquest efecte modulador sobre els mastòcits és
paral lel a la sobreexpressió del receptor EP2. D’aquests resultats sorgeix la
hipòtesi que la PGE2 podria interactuar amb els receptors EP2 dels mastòcits de la
superfície bronquials i així exercir una acció protectora enfront la patologia
impulsada pels al lèrgens en contactar amb les vies respiratòries. El coneixement
precís d’aquests mecanismes sens dubte ajudarà a descobrir potencials molècules
diana contra l'asma.
L'objectiu general d'aquesta tesi és establir preclínicament la rellevança del
receptor EP2 mastocitari en l’efecte beneficiós de la PGE2 en l'asma al lèrgica, i
descobrir els mecanismes moleculars que resulten d’aquesta activació selectiva del
receptor. Per aconseguir aquest objectiu hem dut a terme diversos abordatges in
vitro i in vivo.
En primer lloc, determinar el patró d’expressió dels receptors EP de la PGE2 en
diferents poblacions de mastòcits humans i murins, i es va avaluar a partir de
llavors (a) si aquestes diferències en l’expressió relativa dels receptors de EP 1-4
influïa en la capacitat de PGE2 per modular la degranulació dels mastòcits i la
mobilització del calci i (b) si els mastòcits humans es van comportar de manera
similar als murins en diferents escenaris d'expressió dels receptors EP. Els resultats
van apuntar a EP2 com el principal contribuent a l’efecte inhibitori de la PGE2 sobre
els mastòcits murins i humans. Quan EP2 es va suggerir com a el receptor primari
de protecció, vam abordar la importància de l’activació selectiva d’EP2 (a) en la protecció de la patologia de les vies respiratòries induïda per HDM en ratolins, i (b)
la correlació d’aquesta patologia amb la capacitat de l’agonista selectiu EP2 en
prevenir l’activitat de mastòcits in vivo. Hem demostrat que un agonista selectiu
d’EP2 impedia el desenvolupament de l’AHR i la inflamació, i que aquest efecte
estava relacionat amb la capacitat d'aquesta acció de l’agonista selectiu per atenuar
l’activitat dels mastòcits de les vies respiratòries. A continuació, es van estudiar
possibles mecanismes inhibitoris de senyalització implicats en aquest efecte de
bloqueig intervingut per EP2. Hem observat que l’agonisme d’EP2 inhibeix in vivo i
in vitro, l'activitat dels mastòcits. Hem descrit que la interacció amb PGE2-EP2
inhibeix la degranulació mastocitària a través de la supressió de la mobilització de
calci intervinguda per la inhibició de la via Src-Fyn, i cAMP/PKA.
Les nostres observacions ressalten que l’eix "la PGE2" - "l’EP2 mastocitari" - "les
vies respiratòries" és una via endògena que condueix a una protecció natural contra
la patologia de les vies respiratòries induïda pels aeroal lergens i ajuda a dilucidar
els mecanismes precisos que descobreixen molècules diana objectiu de possibles
nous tractaments antiasmàtics.Allergic asthma is a chronic respiratory disease with a high prevalence in developed countries. Current treatments do not halt the underlying allergic process and do not always control the symthomps of the disease. The most effective treatment is the use of glucocorticoids, which are based on chemical modifications of potent natural endogenous anti-inflammatory hormones. Studying endogenous anti-inflammatory pathways to explore new therapeutic targets is an efficient experimental strategy to uncover potential novel targets against asthma. One of such endogeneous pathways are cyclooxygenase (COX)-mediated. Prostaglandin (PG) PGE2, a COX product, has been suggested to exert a protective effect in the lungs. Notably, experimental studies with asthma patients revealed that inhaled PGE2 reduces airway hyperresponsiveness and inflammation. This protective PGE2 effect has also been demonstrated, directly and indirectly, in mice sensitized to OVA or HDM. The mechanisms underlying the beneficial effect of PGE2 in asthma are not understood. One of the most consistent features of PGE2 is its ability to modulate mast cell activity in vitro. Our recent in vivo studies showed that PGE2 also prevents mast cell activity in HDM sensitized mice and that this mast cell modulatory effect was paralleled by EP2 receptor overexpression. These results brought up the hypothesis that PGE2 might interact with EP2 receptor on the bronchial mast cells surface to exert a protective action against allergen-driven airway pathology. The precise understanding of such mechanisms will certainly help uncover potential anti-asthma target molecules along the way. The general objective of this thesis was to establish preclinically the relevance of the mast cell EP2 receptor to PGE2 beneficial effect in allergic asthma, and to uncover molecular mechanisms resulting from this receptor selective activation. To achieve this objective we have undertaken several in vitro and in vivo approaches. We first determined the PGE2 EP receptors expression pattern on different human and murine mast cell population, and thereafter assessed (a) whether such differences in the relative expression of EP receptors 1 to 4 influenced the ability of PGE2 to modulate mast cells degranulation and calcium mobilization, and (b) whether human mast cells behaved similarly to murine mast cells under different EP receptors expression scenarios. The results pointed at EP2 as the main contributor to mediate the inhibitory effect of PGE2 on both murine and human mast cells. Once EP2 had been suggested to be the primary protective receptor, we addressed the relevance of selective EP2 activation to (a) protection against HDMinduced airway pathology in mice, and (b) correlation of such pathology to the ability of selective EP2 agonism to prevent mast cells activity in vivo. We showed that a selective EP2 agonist prevented AHR and inflammation from developing, and that such effect was linked to the ability of such selective agonistic action to attenuate airway mast cell activity. We then studied potential inhibitory signaling mechanisms involved in such EP2-mediated blocking effect. We observed that EP2 agonism inhibited in vivo and in vitro, mast cell activity. We described that the PGE2-EP2 interaction on mast cells inhibiting mast cell degranulation through the supression of calcium influxes mediated by an inhibition of the Src-Fyn pathway, and cAMP/PKA. Our observations highlight that the “PGE2”-“mast cells EP2”-“airway” axis is an endogeneous pathway leading to natural protection against aeroallergens-induced airway pathology, and helps elucidate the precise mechanisms that will uncover clue molecules to be targeted by potential novel antiasthma treatments.
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Madouri, Fahima. "Asthme allergique induit par un allergène d’acarien, House Dust Mite (HDM) : rôles de la caspase-1 et de la protéine kinase C thêta (PKC-θ)." Thesis, Orléans, 2014. http://www.theses.fr/2014ORLE2055/document.
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Des études menées au laboratoire avaient démontré un rôle critique de l’inflammasome NLRP3 dans l’asthme allergique en réponse à l’ovalbumine en absence d’adjuvant. Mes travaux de thèse ont porté sur le rôle de NLRP3 et de la caspase-1 dans un modèle murin d’inflammation pulmonaire induite par l’allergène d’acarien HDM. Nous avons montré un rôle régulateur de la caspase-1 dépendant de l’inflammasome NLRP3 et la molécule adaptatrice ASC mais pas de l’inflammasome NLRC4. Cette régulation de la réponse allergique se caractérise par une augmentation de l’infiltration des éosinophiles, de l’hyperréactivité bronchique et de la production des cytokines de type Th2 telles que l’IL-4, l’IL-5, l’IL-13 et l’IL-33 dans les poumons. Nous avons montré que les mécanismes responsables de cette régulation sont associés à l’IL-33 produite par les macrophages et que la neutralisation de l’IL-33 par administration locale de la protéine de fusion au récepteur ST2 (muST2-Fc) atténue les caractéristiques de l’asthme allergique. Ces résultats suggèrent que l’activation de la caspase-1 réduit la production d’IL-33 in vivo et régule ainsi la réponse l’inflammation pulmonaire induite par HDM et la réponse Th2. D’autre part, nous nous sommes intéressés au rôle de la Protéine Kinase C thêta (PKC-θ) dans ce même modèle d’inflammation pulmonaire. Nous avons démontré que PKC-θ joue non seulement un rôle protecteur dans l’asthme allergique mais également un rôle critique pour la prolifération et l’activation des cellules lymphoïdes innées (ILC2). D’autre part, l’inhibition de PKC-θ in vivo par administration orale de son inhibiteur spécifique C20 (BIX02656) atténue l’inflammation pulmonaire et la production d’IL-5 et d’IL-13. Nous suggérons que PKC-θ est impliquée dans la différenciation des Th2 et des ILC2 via un mécanisme dépendant des facteurs de transcription IRF4 et NFAT-1. Au total, mes travaux de thèse mettent en exergue deux molécules IL-33 et PKC-θ qui pourraient constituer des cibles thérapeutiques potentiellesStudies from our laboratory have shown a critical role of NLRP3 inflammasome in response to ovalbumin allergen. In the present study we investigate the role of NLRP3 and caspase-1 in a mouse model of pulmonary inflammation induced by HDM. We have shown a regulatory role of caspase-1 dependant of the NLRP3 inflammasome and the adaptator molecule ASC but not NLRC4. The regulation of the allergic response is characterized by an increase of eosinophilia, bronchial hyperreactivity and Th2 cytokines production (IL-4, IL-5, IL-13 and IL-33) in lungs. We have shown that mechanisms responsible of this regulation are associated with IL-33 production by macrophages and that neutralization of IL-33 by local administration of a fusion protein of the ST2 receptor (muST2-Fc) reduce characteristics of asthma. These results suggest that caspase-1 activation reduce IL-33 production in vivo regulating lung inflammation and Th2 response induced by HDM. Moreover, we investigate the role of the Protein Kinase C theta (PKC-θ) in allergic airway inflammation. We have demonstrated that PKC-θ plays a protective role in allergic asthma but is critical for the activation and proliferation of innate lymphoid cells (ILC2). In addition, in vivo inhibition by oral administration of PKC-θ specific inhibitor C20 (BIX02656) reduces pulmonary inflammation with IL-5 and IL-13 production. We suggest that PKC-θ is implicated in Th2 and ILC2 differenciation by a mechanism dependant on transcription factors IRF4 and NFAT-1. Finally, my thesis projects describe IL-33 and PKC-θ as potential therapeutic targets for allergic lung inflammation
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Filipe, Daniel V. "Modifying and Measuring the Stiffness of a Regenerative Cardiac Scaffold In Vitro." Digital WPI, 2010. https://digitalcommons.wpi.edu/etd-theses/1098.
Full textAbstract:
"The stiffness of scaffolds used in surgical ventricular restoration may play an important role in the degree to which they facilitate regeneration of functional cardiac tissue. The stiffness of the scaffold influences the phenotype of cells which are present in it as well as their ability to deform the scaffold. The goal of this study was to evaluate in vitro methods to characterize and alter the stiffness of new scaffold materials. Membrane inflation testing, an in vitro mechanical testing method, was evaluated in this study because of its ease of use and the similar mode of loading which it shares with scaffolds implanted in vivo. The structural stiffness of two scaffold materials, urinary bladder matrix and Dacron, were determined in vivo and using membrane inflation testing. Despite higher tensions and lower area stretch ratios for scaffolds tested using membrane testing, similar changes in structural stiffness between the two materials were found for both methods (5.6 ± 3.3 fold in vivo, 5.0 ± 1.0 in vitro). This finding demonstrated that membrane inflation testing is a useful in vitro method for measuring changes in structural stiffness between scaffold materials with a level of sensitivity similar to that which is measured in vivo. Membrane inflation testing was used to assess the effectiveness of altering scaffold stiffness through exposure to various cell culture conditions. Incubation of a biological membrane in cell culture media resulted in a drastic decrease in the elastic modulus from its initial value (3.55 ± 0.52 MPa) after 2 weeks (1.79 ± 0.30 MPa), 4 weeks (1.04 ± 0.09 MPa), and 10 weeks (0.014 ± 0.01 MPa). When fibroblasts were cultured on the scaffolds for 10 weeks an increase in elastic modulus (0.134 ± 0.05 MPa) over scaffolds incubated in culture media for the same amount of time was observed. The increase in elastic modulus due to the presence of fibroblasts was accompanied by an increase in the percentage of collagen in the samples (54.1 ± 5.1 % without fibroblasts, 83.2 ± 5.1 % with fibroblasts). Contrary to expectation, addition of ascorbic acid to the media to increase production of collagen by the fibroblasts resulted in a decrease in elastic modulus (0.030 ± 0.01 MPa) compared to scaffolds cultured with fibroblasts in standard media and a decrease in the amount of enzymatically degraded collagen (40.8 ± 4.7 % without ascorbic acid, 21.1 ± 3.3 % with ascorbic acid). Regeneration of cardiac tissue after a myocardial infarction is a complicated process which is influenced by a myriad of different factors. Future studies investigating the exact role which substrate stiffness has on regeneration will be essential to the development of improved cardiac scaffolds. Characterization of the stiffness of these scaffolds by membrane inflation and manipulation through exposure to cell culture conditions are powerful approaches to facilitate future studies."
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Costa, António Maria Oom de Sousa da. "Gestão de capacidade em serviços : a gestão de capacidade num Hospital de Dia." Master's thesis, 2015. http://hdl.handle.net/10400.14/21709.
Full textAbstract:
A economia dos serviços tem vindo a aumentar desde os anos 90. Estes são, hoje em dia, uma força propulsora da economia de cada país.
Dentro desta área dos serviços, a gestão de capacidade é um conceito que tem vindo a ser estudado e desenvolvido ao longo dos anos. A gestão de capacidade é crucial para os serviços, no sentido em que emprega estratégias com o objectivo de dar resposta à procura e ao mesmo tempo é responsável por manter o serviço com níveis de qualidade e eficiência constantes.
O presente estudo debruça-se sobre a gestão de capacidade num ambiente hospitalar, mais concretamente num hospital de dia médico oncológico/polivalente e tem como objectivo identificar problemas nos processos existentes e sugerir soluções.
O trabalho foi desenvolvido num contexto de estágio, que decorreu no Hospital Beatriz Ângelo durante o último quadrimestre de 2014.
A metodologia utilizada pelo autor foi a de investigação-ação (AR), o que permite desenvolver estratégias para a resolução de problemas através da colaboração dos diversos stakeholders presentes na organização.
Foi possível chegar à conclusão de que existe um problema de gestão de capacidade na área estudada. De entre as sugestões feitas destaca-se a necessidade de existir um planeamento conjunto das diversas áreas que integram os processos existentes no hospital de dia.
Espera-se com este estudo, contribuir para o desenvolvimento da literatura que engloba a Gestão de Operações e a gestão de capacidade, especialmente num contexto hospitalar.The services economy has been increasing since the 90s. They are now a driving force of the economy of each country. Within this area of services, capacity management is a concept that has been studied and developed over the years. Capacity management is crucial for the services in that it employs strategies in order to meet demand and at the same time is responsible for maintaining the service quality and efficiency levels constant. This study focuses on the capacity management in a hospital setting, specifically in an oncology/multipurpose day hospital and aims to identify problems in existing processes and suggest solutions. The study was conducted in a stage context. This took place at the Hospital Beatriz Ângelo during the last four months of 2014. The methodology used by the author was the action-research (AR), which allows developing strategies for problem solving through collaboration with various stakeholders present in the organization. It was possible to conclude that there is a capacity management issue in the study area. Among the suggestions there is the need for a joint planning of the various areas that are part of existing processes on a day basis. It is hoped that this study contributes to the development of literature which includes Operations Management and capacity management, especially in a hospital setting.
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Chou, Chiu-Chu, and 周秋菊. "Remote Lab Application Measuring HDMI system interface ---An Example HDMI." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/63203173973499667655.
Full textAbstract:
碩士國立宜蘭大學
多媒體網路通訊數位學習碩士在職專班
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This paper presents an integrated laboratory equipment and the use of remote desktop control to create a remote control laboratory to complete the amount of High Definition Multimedia Interface measurement methods, By the measurement process enables engineers to understand the changes in the general course of the measured waveform to increase the engineers to capture waveforms and increased experience to judge the correctness of measurements. Technically the use of existing operating platform - Microsoft embedded remote functionality to create a remote desktop control laboratory concept. Through the network link, Link oscilloscope and Device under Test, Rapid remote functions by setting and can quickly establish a remote desktop control laboratory to complete HDMI signal measurements. Over Remote Desktop Connection can enhance learning efficiency engineer and is up to limit the convenience of the measurement area, through a network connection to the remote desktop remote. You can get real-time data. As determine the validity of the testing process. In this experiment include a major computer, Oscilloscopes and measuring the amount of the required software, hardware and Device under Test (DUT). The computer connect network to connect the Device under Test and oscilloscope. Through the Windows embedded Remote Desktop control function. Creation a data base store experiment waveform and data. At the same time use a webcam and Remote Desktop Connection to increase the accuracy of measurements and I reduce the loss of laboratory equipment.