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Dissertations / Theses on the topic 'Protein expression in E'

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Published: 4 June 2021

Last updated: 1 February 2022

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1

Lin, Da. "Regulation of MICA and MICB expression." Thesis, University of Oxford, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.509978.

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2

Sarkar, Mohosin M. "Engineering Proteins with GFP: Study of Protein-Protein Interactions In vivo, Protein Expression and Solubility." The Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1261418776.

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3

Wong, Chung Kai. "The DIX domain protein Ccd1 inhibits JNK activation by axin and dishevelled through distinct mechanisms /." View abstract or full-text, 2004. http://library.ust.hk/cgi/db/thesis.pl?BICH%202004%20WONG.

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Thesis (M. Phil.)--Hong Kong University of Science and Technology, 2004.<br>Includes bibliographical references (leaves 60-68). Also available in electronic version. Access restricted to campus users.

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4

Koscky, Paier Carlos Roberto 1983. "Padronização da expressão heterologa e de modelo de ensaio de atividade para a proteina quinase humana S6K." [s.n.], 2009. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314787.

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Orientador: Nilson Ivo Tonin Zanchin<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia<br>Made available in DSpace on 2018-08-14T12:40:52Z (GMT). No. of bitstreams: 1 KosckyPaier_CarlosRoberto_M.pdf: 3760581 bytes, checksum: 99331529324819b59a4360d60efd9b9a (MD5) Previous issue date: 2009<br>Resumo: A quinase de 70 kDa da proteína ribossomal S6, isoforma 1 (S6K1), é uma fosfoproteína implicada na regulação de genes relacionados ao controle da tradução em mamíferos e possui uma forma nuclear (a1) e uma citoplasmática (a2). A fosforilação do seu principal alv

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5

Kane, Émilie. "Protein-protein regulation of calsequestrin expression in cardiomyocytes." Thesis, McGill University, 2012. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=107782.

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Heart failure is the leading cause of death in both men and women of Western countries. The pathophysiology of heart failure is associated with abnormalities in intracellular calcium control. Calsequestrin (CSQ2), a calcium storage protein in cardiomyocytes, is negatively regulated by the transcription factor Egr-1 thus altering calcium availability for cardiac contraction/relaxation. Here, we tested the hypothesis that the proteins complexed to Egr-1 and/or their post-translational modifications would affect regulation of CSQ2 expression. Egr-1 and Sp1 compete for binding at the CSQ2 promoter

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6

Lexander, Helena. "Protein expression in prostate cancer /." Stockholm, 2006. http://diss.kib.ki.se/2006/91-7140-617-4/.

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7

Tweedle, Elizabeth. "Protein expression in colorectal cancer." Thesis, University of Liverpool, 2011. http://livrepository.liverpool.ac.uk/4773/.

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Introduction: Colorectal cancer is the second most common UK cancer. Biomarkers which predict survival may be valuable for targeting adjuvant therapy and can provide insights into tumour biology. Small and early cancers are being diagnosed more commonly in the UK population due to the introduction of population-based colorectal cancer screening in 2005. Analysis of resected small (≤20mm across) tumours in Liverpool has established that flat and depressed morphology can predict advanced stage at presentation. Proteomic analysis of small cancers was conducted with the aim of generating biomarker

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8

Magnusson, Kristina. "Protein Expression Profiling of Cancer Biomarkers." Doctoral thesis, Uppsala universitet, Institutionen för immunologi, genetik och patologi, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-265513.

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The Human Protein Atlas project is a Swedish research initiative that uses antibody-based proteomics for large scale protein profiling in human tissues and cells. Affinity-purified antibodies are produced within the project and used for immunohistochemical staining on tissue micro arrays (TMAs) in order to map the human proteome and publish the result in a protein atlas (www.proteinatlas.org). In this thesis, TMAs were used for analysis of protein expression patterns in order to identify and explore potential biomarkers of clinical relevance. In Paper I, protein expression of SATB2 was studied

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9

Wesselhoeft, R. Alexander(Robert Alexander). "Synthetic circular RNA for protein expression." Thesis, Massachusetts Institute of Technology, 2019. https://hdl.handle.net/1721.1/122710.

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Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, 2019<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (pages 111-126).<br>Messenger RNA (mRNA) has broad potential for therapeutic and engineering applications. One fundamental limitation of mRNA is its relatively short half-life in biological systems, effected in part by rapid exonuclease-mediated degradation upon delivery. Circular RNA (circRNA), a type of single-stranded RNA with a contiguous structure that lacks the end motifs necessary for exonuclease recognition, may be resistant

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10

Anderson, Ross Calley. "Expression and characterisation of a novel poly(A)-binding protein, PABP5." Thesis, University of Edinburgh, 2010. http://hdl.handle.net/1842/5942.

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The poly(A)-binding proteins (PABPs) are a family of eukaryotic RNA-binding proteins with key roles in mRNA translation and stability. The molecular function of PABPs have been largely revealed through study of the prototypical cytoplasmic poly(A)-binding protein, PABP1. Thus, little is known regarding other PABP family members. PABP5 contains four RNA-recognition motifs characteristic of the cytoplasmic PABPs yet is structurally distinct as it lacks a portion of the C-terminus. This region contains a proline-rich section linked to a globular domain that facilitates a number of protein-protein

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11

Brazier, Hannah. "An investigation into the structural role of the CCR4-NOT complex in mRNA stability." Thesis, University of Oxford, 2017. https://ora.ox.ac.uk/objects/uuid:9ffe2809-a0d2-437e-8506-f56af0c0a495.

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The CCR4-NOT complex is a global regulator of gene expression which controls mRNA levels by removing the poly-(A) tail, a step known as deadenylation and one that constitutes the rate-limiting step in mRNA decay. The human complex is comprised of eight stably associated CNOT subunits where CNOT1 forms the scaffold onto which CNOT2-11 bind. Although much has been learnt about the CCR4-NOT complex, questions still remain. Thus, this study focused on a number of sub-complexes of CNOT subunits and associated proteins to determine the mode of interaction with a hope to explore the mechanism of dead

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12

Berkes, Charlotte Amelia. "Elucidating the mechanisms by which MyoD establishes muscle-specific gene expression /." Thesis, Connect to this title online; UW restricted, 2004. http://hdl.handle.net/1773/5071.

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13

Gunnarsson, Ida. "Deriving Protein Networks by Combining Gene Expression and Protein Chip Analysis." Thesis, University of Skövde, Department of Computer Science, 2002. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-706.

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<p>In order to derive reliable protein networks it has recently been suggested that the combination of information from both gene and protein level is required. In this thesis a combination of gene expression and protein chip analysis was performed when constructing protein networks. Proteins with high affinity to the same substrates and encoded by genes with high correlation is here thought to constitute reliable protein networks. The protein networks derived are unfortunately not as reliable as were hoped for. According to the tests performed, the method derived in this thesis does not perfo

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14

Delucchi, Anthony Benjamin. "Bacterial expression of radio-labeled recombinant proteins for studying AHR signalling." Scholarly Commons, 2001. https://scholarlycommons.pacific.edu/uop_etds/550.

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The ligand activated transcription factor Aryl Hydrocarbon Receptor (AHR) forms a DNA binding heterodimer with the Aryl Hydrocarbon Nuclear Translocator (ARNT) in response to planar aromatic hydrocarbons. In addition to AHR and ARNT there are at least three other proteins involved in AHR signaling. These proteins are the co-chaperone p23, Ara-9 and two molecules of Heat Shock Protein-90 (HSP-90). This study documents the production of Ara-9 and C∆418 (an ARNT deletion construct) in a modified thioredoxin fusion system. These proteins were expressed in a system that allowed for removal from the

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15

Nagy, Noémi. "Expression and function of the small immune adaptor protein SAP /." Stockholm, 2006. http://diss.kib.ki.se/2006/91-7357-029-X/.

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16

Byström, Jonas. "Eosinophil Cationic Protein : Expression Levels and Polymorphisms." Doctoral thesis, Uppsala University, Department of Medical Sciences, 2002. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2059.

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<p>The eosinophil cationic protein (ECP) is usually associated with the eosinophil granulocyte. In this thesis the presence and production of this protein has been studied in two other cells. The circulating monocyte was found to contain ECP mRNA and small amounts of ECP, one thousand times less than that found in the eosinophil. The production decreased by differentiation of the myelomonoblastic cell line U937 into a macrophage phenotype. Submucosal lung macrophages did not stain for ECP and alveolar macrophages did not contain ECP mRNA. The circulating neutrophil contains ECP at a level hund

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17

Gutierréz, Pablo 1977. "Structural studies of regulators of protein expression." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=85912.

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In most species, highly sophisticated global regulatory networks regulate the expression of genes in response to environmental and physiological demands. The mechanisms devised control virtually every event involved in transcription and translation, as well as influencing mRNA degradation, protein stability, protein localization, protein-protein interactions, and protein function. In order to understand the general mechanism used by several organisms to control gene expression, three regulatory proteins were investigated using nuclear magnetic resonance (NMR) techniques. The systems stu

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18

Xu, Jianhua. "Pokeweed antiviral protein gene, expression and applications." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0006/NQ27754.pdf.

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19

Rushlow, Walter James. "Characterization of CArG-binding protein gene expression." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq31096.pdf.

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20

Lisgo, Steven Newton. "Human TBX22 expression and protein-DNA interactions." Thesis, University of Newcastle Upon Tyne, 2010. http://hdl.handle.net/10443/1066.

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Cleft palate is one of the most common birth abnormalities. Figures published in 2006 by the American Centres for Disease Control and Prevention, report the incidence of those born in the United States with a cleft palate without the presence of a cleft lip (CPI) to be 6.39 for every 10000 in the three years between 1999 to 2001 and for cleft lip in association with a cleft palate (CLP) to be even greater - 10.48 per 10000 live births. In 2001, Braybrook and colleagues reported that mutations in the TBX22 gene cause X-linked cleft palate (CPX), a disease characterised by a cleft of the seconda

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21

Deacon, Sarah Elizabeth. "Protein expression and engineering of galactose oxidase." Thesis, University of Leeds, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.494592.

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22

Pratt, Kathryn Alice. "Expression of wheat gluten protein in yeast." Thesis, University of Kent, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.236722.

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23

Borland, Gillian. "CD44 : an analysis of protein isoform expression." Thesis, University of Strathclyde, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.310906.

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24

Liu, Honglei. "Protein expression in Pseudomonas putida F1 biofilms." Thesis, King's College London (University of London), 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.440481.

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25

Ford, Melanie. "Cellular prion protein expression in the mouse." Thesis, King's College London (University of London), 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.249698.

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26

Yuk, Inn Huam Yvonne. "Protein expression and glycosylation in CHO cells." Thesis, Massachusetts Institute of Technology, 2001. http://hdl.handle.net/1721.1/8201.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 2001.<br>Includes bibliographical references (p. 193-207).<br>A successful mammalian cell culture process depends on sufficient expression and correct glycosylation of the recombinant product. Low product titer and inconsistent protein glycosylation constitute two major problems frequently encountered in biopharmaceutical production. Using Chinese Hamster Ovary (CHO) cells expressing recombinant human interferon-gamma (IFN-y) as the model system, this thesis investigated strategies that can potentially enhan

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27

Zemanay, Widaad. "Altered protein expression patterns in oesophageal cancer." Doctoral thesis, University of Cape Town, 2009. http://hdl.handle.net/11427/3157.

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Includes abstract.<br>Includes bibliographical references (leaves 125-143).<br>Oesophageal squamous cell carcinoma presents a significant health burden in South Africa. It is one of the most common causes of cancer-related mortality of South African black males, as a result of its asymptomatic progression leading to late diagnosis and poor prognosis. The aim of this study was to identify membrane or membrane-associated proteins that are expressed at different levels in oesophageal tumour tissue when compared to normal tissue. The identification of such proteins would be an important step towar

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28

Yang, Xu. "Quantitative Approaches for Protein Differential Expression Analysis." Thesis, Virginia Tech, 2009. http://hdl.handle.net/10919/36172.

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In this work, tandem mass spectrometry (MS/MS)-based quantitative protocols were developed to facilitate differential protein expression analysis and biomarker discovery via a two-step sample interrogation strategy: (a) global protein profiling and differential expression analysis by spectral counting; and, (b) biomarker candidate validation by targeted screening, i.e., multiple reaction monitoring (MRM). Preliminary experiments were performed to evaluate the performance of the spectral counting method. The method proved to be applicable for proteins with spectral countsâ ¥2, and a close-to-l

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29

Morgan, Sarah V. "Tight junction protein expression in human astrocytes." Thesis, University of Sheffield, 2016. http://etheses.whiterose.ac.uk/14403/.

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Tight junctions are formed from a complex of different individual proteins. These complexes are expressed by epithelial cells and form an intercellular barrier which restricts and regulates paracellular permeability. Tight junction proteins have also been shown to be expressed in non-epithelial cells which do not form tight junctions, including astrocytes. The function(s) of these proteins within non-epithelial cells, however, remains unclear. This study aims to characterise the expression of tight junction proteins in astrocytes and investigate the function(s) of these proteins in these cells

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30

Dafas, Panagiotis A. "Frequency rule mining for effective protein-protein interaction inference from gene expression and protein structures." Thesis, City University London, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.492255.

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The experimental measurement of gene expression levels has produced preliminary results on the regulation, pathways and networks of genes in cells. Furthermore, the number of available three-dimensional folded structures of proteins increases on a daily basis. The ultimate aim of both genomics and proteomics froni the perspective of bioinformatics, is to map out all the circuits of energy and information processing in life by terms of molecular interactions in a system~tic way with minimal human intervention. In this thesis we propose a new rule mining framework for ill silico inference of pro

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31

Hallal, Samantha. "Characterisation of the zinc fingers of erythroid krüppel-like factor." Connect to full text, 2008. http://ses.library.usyd.edu.au/handle/2123/4030.

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Thesis (Ph. D.)--University of Sydney, 2009.<br>Title from title screen (viewed February 10, 2009). Submitted in fulfilment of the requirements for the degree of Doctor of Philosophy to the School of Molecular and Microbial Biosciences, Faculty of Science. Degree awarded 2009; thesis submitted 2008. Includes bibliographical references. Also available in print form.

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32

Bane, Steven Edward. "Expression and characterization of the human neurokinin 1 receptor from Escherichia coli." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 99 p, 2007. http://proquest.umi.com/pqdweb?did=1342742951&sid=1&Fmt=2&clientId=8331&RQT=309&VName=PQD.

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33

Ndabambi, Nonkululeko. "Recombinant expression of the pRb- and p53-interacting domains from the human RBBP6 protein for in vitro binding studies." Thesis, University of the Western Cape, 2004. http://etd.uwc.ac.za/index.php?module=etd&amp.

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The aim of this thesis was to produce DNA expression constructs and use them to investigate the feasibility of recombinantly expression proteins for future interaction studies between human RBBP6 and p53 and pRb proteins.

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34

Quiroga, Blas III. "Optimization of Protein Expression of an ELP-GFP Fusion Protein In Bacterial Systems." Cleveland State University / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=csu1611791577229689.

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35

Santos, Camila Oresco dos. "Fatores geneticos moduladores da gravidade clinica nas Beta-talassemias : o exemplo da proteina AHSP (Alpha Hemoglobin Stabilizing Protein)." [s.n.], 2006. http://repositorio.unicamp.br/jspui/handle/REPOSIP/313738.

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Orientador: Fernando Ferreira Costa<br>Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas<br>Made available in DSpace on 2018-08-07T17:09:12Z (GMT). No. of bitstreams: 1 Santos_CamilaOrescodos_D.pdf: 28263366 bytes, checksum: 0bb038ac0bb395e9aed9a7089336b2d0 (MD5) Previous issue date: 2006<br>Resumo: AHSP é uma proteína eritróide específica que apresenta afinidade de ligação com a-globinas, estabilizando essas moléculas e dessa forma, evitando a precipitação nos precursores eritróides e bloqueando danos celulares causados pela oxidação de cadeias globínicas.

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36

Polikandriotis, John Anastasios. "Elucidating the regulation of vascular smooth muscle alpha-actin gene expression in fibroblasts." Connect to this title online, 2004. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1078857443.

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Thesis (Ph. D.)--Ohio State University, 2004.<br>Title from first page of PDF file. Document formatted into pages; contains xiv, 177 p.; also includes graphics (some col.). Includes bibliographical references (p. 160-177).

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37

Ogut, Ozgur. "cDNA cloning, protein expression, tissue specific expression pattern and functional characterization of nebulin and nebulette, two homologous striated muscle proteins." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/mq24689.pdf.

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38

Stenberg, Leisa M. "Expression and characterization of a recombinant human factor X/protein C chimeric protein." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape8/PQDD_0015/NQ46432.pdf.

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39

Simpfendörfer, Robert. "Untersuchungen zur Expression von Protein-Tyrosinphosphatasen in Lymphocyten /." Konstanz, 2008. http://opac.nebis.ch/cgi-bin/showAbstract.pl?sys=000259293.

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40

Toufighi, Kiana 1980. "Integrative study of gene expression and protein complexes." Doctoral thesis, Universitat Pompeu Fabra, 2014. http://hdl.handle.net/10803/380907.

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Over the last several decades, the emerging ‘integrated’ view of the cell has triumphed over the ‘one gene/one protein/one function’ paradigm. This is illustrated by the biologically opposite effects of key regulatory proteins in different cell types, in established versus primary cells, and in vitro versus in vivo situations. The persistent theme throughout this dissertation is the integration of a wide range of data sources for the purpose of understanding distinct cellular contexts. We first use circadian expression data from human epidermal stem cells to discover waves of transcripts

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41

Piekny, Alisa J. "Patterns of PKC53E protein expression in Drosophila melanogaster." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp04/mq24692.pdf.

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42

Carlén, Lina. "Characterization of psoriasis lesions by protein expression profiling /." Stockholm : Karolinska institutet, 2006. http://diss.kib.ki.se/2006/91-7140-808-8/.

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43

Lundgren, Caroline. "Endometrial carcinoma : prognostic factors and protein expression profiling /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7140-002-8/.

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44

Nelson, Sarah Kay. "OSMOTIC REGULATION OF RENAL GENE AND PROTEIN EXPRESSION." Thesis, The University of Arizona, 2009. http://hdl.handle.net/10150/192556.

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45

Weeks, Stephen David. "Applications of fusion technologies in recombinant protein expression." Thesis, Lancaster University, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.428646.

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46

McNee, John James. "Expression, purification and characterisation of a knotted protein." Thesis, Imperial College London, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.286718.

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47

Nichol, Donna. "Analysis of receptor interacting protein (RIP140) gene expression." Thesis, Imperial College London, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.434341.

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48

Xu, Xiaodong. "Expression and characterization of HIV-1 envelope protein." Thesis, University of Reading, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.500515.

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We postulated that gp120 and CD4 interaction might expose cryptic epitopes on gp120 that could be immunogenic and so widen the immunogenic response. A fusion protein of a C clade strain of HIV-1 (HIVCN54) gp120 and full-length human CD4 was constructed and expressed using a recombinant baculovirus system. The protein was purified, characterized and used to immunize rabbits. The antiserum generated had an expected anti-gp120 activity and demonstrated a higher capacity than a control serum raised to gp120 alone to block b12 binding, a marker of neutralization. A formal neutralization assay howev

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49

Aboonq, Moutasem Salih. "Activity dependent neuroprotective protein (ADNP) expression and functions." Thesis, University of Liverpool, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.540017.

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50

Dwivedi, Gaurav Dutta. "Cloning and Expression of Streptococcal Recombinant Protein G." Thesis, Umeå universitet, Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet), 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-106723.

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Recombinant Protein G (rPG), an engineered form of streptococcal protein G with a theoretical molecular weight of 22.26 kDa was successfully cloned and expressed in E.coli BL 21(DE3) cells. The albumin binding domain was removed during the gene synthesis to avoid unspecific binding. This recombinant form of protein G contains only the IgG binding domains along with the 6X histidine tag at the N terminal. The removal of non-specific domains maximizes the specificity of IgG binding through the Fc region. The recombinant protein G was purified through heat treatment and using immobilized metal af

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