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Journal articles on the topic "Separateur de cellule"

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Mejstrikova, Ester, Vendula Pelkova, Michaela Reiterová, Martina Sukova, Zuzana Zemanova, Elena Vodickova, Vit Campr, et al. "Composition of Cellular Subsets by Flow Cytometry Identifies Differences Between MDS Subtypes and Aplastic Anemia but No Differences Are Identified Between Cases with and without Monosomy 7." Blood 114, no. 22 (November 20, 2009): 3802. http://dx.doi.org/10.1182/blood.v114.22.3802.3802.

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Abstract Abstract 3802 Poster Board III-738 Introduction Monosomy 7 or del(7q) are frequent cytogenetic abnormalities in children with myelodysplastic syndrome (MDS) and associates with poor prognosis. MDS globally affects all cellular subsets in bone marrow and in peripheral blood. We asked whether flow cytometry (FC) can separate individual subtypes of MDS from each other and from aplastic anemia (SAA) and whether in individual subtypes of childhood MDS can separate patients with and without monosomy 7. Patients/analyzed parameters In total we analyzed 94 children with centrally analyzed immunophenotype in the reference lab who were diagnosed and treated for MDS or SAA between 1998 and 2009. In total we analyzed 14 patients with refractory cytopenia, 37 patients with advanced forms of MDS (JMML 10, RAEB 25, CMML 2) and 43 patients with SAA. Monosomy 7/del(7q) was present in 17 patients (RC 6, JMML 3, RAEB 8). Analyzed parameters were as follows: B cells, CD10+CD19+, CD19+45dim/neg, CD19+34+, CD19/CD34 ratio, CD34+, CD117 cells, CD34+38dim/neg, CD3+, CD3+4+, CD3+8+, CD3+HLADR+. Statistics We analyzed all parameters using non parametric tests (Mann-Whitney, Kruskal Wallis) and principal component analysis (PCA). Results Principal component analysis of all analyzed patients together clearly separates advanced forms of MDS from RC and SAA, the most contributing factor being the number of CD34 and CD117+ cells. In non parametric statistics following factors significantly differ among MDS subtypes and SAA (Kruskal-Wallis): CD19, CD117, CD34, CD3, CD3+4+, CD8+ and CD3+HLADR+. RC and SAA patients are separated mainly by the number of B cells and the CD34:CD19 ratio. In addition, the following parameters differ between RC and SAA (Mann-Whitney): CD34, CD117 and CD3+HLADR+. Unlike the CD34:CD19 ratio, the number of CD19+34+ precursors does not differ between RC and SAA patients. Patients with monosomy 7 do not differ from the remaining patients when all MDS patients are analyzed together or separately in the respective subgroups (RC, non RC, JMML) by PCA or by non parametric statistics. Conclusion PCA separates advanced MDS forms from RC and SAA. Advanced forms of MDS are characterized by increased percentage of CD34+ and CD117+ cells compared to RC and SAA patients. The global reduction of B cell progenitor compartment is pronounced especially in non-JMML cases of MDS, whereas SAA patients typically present with isolated reduction of cells at early stages (CD19+34+) of B cell development. Patients with monosomy 7 cluster within the respective disease category, they do not form own cluster in PCA. Supported by MSMT VZ MSM0021620813, MZO 00064203 VZ FNM, MZO VFN2005, IGA NR/9531-3, NPV 2B06064. Disclosures: No relevant conflicts of interest to declare.
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Banani, Salman F., Allyson M. Rice, William B. Peeples, Yuan Lin, Saumya Jain, Roy Parker, and Michael K. Rosen. "Compositional Control of Phase-Separated Cellular Bodies." Cell 166, no. 3 (July 2016): 651–63. http://dx.doi.org/10.1016/j.cell.2016.06.010.

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Sharpe, P. T., R. M. Sharrard, and D. J. Watts. "Polypeptide compositions of amoebae of the cellular slime mould Dictyostelium discoideum separated by partitioning during development." Bioscience Reports 5, no. 2 (February 1, 1985): 121–27. http://dx.doi.org/10.1007/bf01117058.

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Amoebae of the cellular slime mould Dictyostelium discoideum at 8 h or 10 h development were separated into two populations by countercurrent distribution in a dextran-poly(ethylene glycol), two-phase system. Two-dimensional, polyacrylamide-gel electrophoresis was then used to separate the polypeptides from the populations of amoebae. The two populations of amoebae at 8 h development differed sn polypeptide composition as did the populations separated at 10 h development. This confirms that cell differentiation is initated in D. discoideum prior to 8 h development.
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Bian, Yue Gen, Jing Huan Su, and Xue Mei Song. "A CTCSS Approach to Mitigate Interference in VHF Cellular Network." Advanced Materials Research 926-930 (May 2014): 2763–66. http://dx.doi.org/10.4028/www.scientific.net/amr.926-930.2763.

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The VHF radio can overcome line-of-sight transmission by “repeaters,” which pick up weak signals, amplify and retransmit them on a different frequency. However, repeaters can interfere with each other unless they are far enough apart or transmit on sufficiently separated frequencies. In this paper, we propose such a system using the continuous tone-coded squelch system (CTCSS) to mitigate interference problems. This system associates to each repeater a separate sub-audible tone that is transmitted by all users who wish to communicate through that repeater. The repeater responds only to receive signals with its specific tone.
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Filippini, Ilario, Alessandro Enrico Cesare Redondi, and Antonio Capone. "Beyond Cellular Green Generation: Potential and Challenges of the Network Separation." Mobile Information Systems 2017 (2017): 1–11. http://dx.doi.org/10.1155/2017/7149643.

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This article introduces the ideas investigated in the BCG2 project of the GreenTouch consortium. The basic concept is to separate signaling and data in the wireless access network. Transmitting the signaling information separately maintains coverage even when the whole data network is adapted to the current load situation. Such network-wide adaptation can power down base stations when no data transmission is needed and, thus, promises a tremendous increase in energy efficiency. We highlight the advantages of the separation approach and discuss technical challenges opening new research directions. Moreover, we propose two analytical models to assess the potential energy efficiency improvement of the BCG2 approach.
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Bailey, LeeAnn O., Matthew L. Becker, Jean S. Stephens, Nathan D. Gallant, Christine M. Mahoney, Newell R. Washburn, Aarti Rege, Joachim Kohn, and Eric J. Amis. "Cellular response to phase-separated blends of tyrosine-derived polycarbonates." Journal of Biomedical Materials Research Part A 76A, no. 3 (March 1, 2006): 491–502. http://dx.doi.org/10.1002/jbm.a.30527.

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Bollmann, Franziska, Jan-Niklas Dohrke, Christian A. Wurm, Daniel C. Jans, and Stefan Jakobs. "Mitochondrial Protein Abundance Gradients Require the Distribution of Separated Mitochondria." Biology 10, no. 7 (June 23, 2021): 572. http://dx.doi.org/10.3390/biology10070572.

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Mitochondria are highly dynamic organelles that interchange their contents mediated by fission and fusion. However, it has previously been shown that the mitochondria of cultured human epithelial cells exhibit a gradient in the relative abundance of several proteins, with the perinuclear mitochondria generally exhibiting a higher protein abundance than the peripheral mitochondria. The molecular mechanisms that are required for the establishment and the maintenance of such inner-cellular mitochondrial protein abundance gradients are unknown. We verified the existence of inner-cellular gradients in the abundance of clusters of the mitochondrial outer membrane protein Tom20 in the mitochondria of kidney epithelial cells from an African green monkey (Vero cells) using STED nanoscopy and confocal microscopy. We found that the Tom20 gradients are established immediately after cell division and require the presence of microtubules. Furthermore, the gradients are abrogated in hyperfused mitochondrial networks. Our results suggest that inner-cellular protein abundance gradients from the perinuclear to the peripheral mitochondria are established by the trafficking of individual mitochondria to their respective cellular destination.
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Arancibia, Ramón A., and Carl E. Motsenbocker. "ENZYME ACTIVITY IN TABASCO FRUIT SEPARATION ZONES." HortScience 31, no. 5 (September 1996): 746a—746. http://dx.doi.org/10.21273/hortsci.31.5.746a.

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Red-mature Tabasco (Capsicum frutescens) fruit (`McIlhenny Select') normally separate easily at the junction of the fruit and receptacle or calyx. Differences in fruit detachment force (FDF) between two lines, one that separates readily (`McIlhenny Select') and one that does not (`Hard Pick'), have been reported previously (Motsenbocker et al., 1995). In this study, enzyme activity from the detachment area was analyzed by viscosity reduction. The reaction mixture was 0.3% pectin in 20 mm NaAc, pH 5.5, for polygalacturonase (PG) and 0.6% carboxymethyl cellulose (CMC) in 20 mm NaPO4, pH 6.0, for cellulase. Preliminary data indicated that PG and cellulase enzyme activity increased during fruit ripening in both lines. Only cellulase activity, however, correlated with FDF. In addition, the activity of both enzymes was higher in the `McIlhenny Select' line than the `Hard Pick' line at the orange and red-mature stages.
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Oftung, Fredrik, Gro Ellen Korsvold, Audun Aase, and Lisbeth M. Næss. "Cellular Immune Responses in Humans Induced by Two Serogroup B Meningococcal Outer Membrane Vesicle Vaccines Given Separately and in Combination." Clinical and Vaccine Immunology 23, no. 4 (February 10, 2016): 353–62. http://dx.doi.org/10.1128/cvi.00666-15.

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ABSTRACTMenBvac and MeNZB are safe and efficacious outer membrane vesicle (OMV) vaccines against serogroup B meningococcal disease. Antibody responses have previously been investigated in a clinical trial with these two OMV vaccines given separately (25 μg/dose) or in combination (12.5 and 12.5 μg/dose) in three doses administered at 6-week intervals. Here, we report the results from analyzing cellular immune responses against MenBvac and MeNZB OMVs in terms of antigen-specific CD4+T cell proliferation and secretion of cytokines. The proliferative CD4+T cell responses to the combined vaccine were of the same magnitude as the homologous responses observed for each individual vaccine. The results also showed cross-reactivity in the sense that both vaccine groups receiving separate vaccines responded to both homologous and heterologous OMV antigen when assayed for antigen-specific cellular proliferation. In addition, a multiplex bead array assay was used to analyze the presence of Th1 and Th2 cytokines in cell culture supernatants. The results showed that gamma interferon, interleukin-4 (IL-4), and IL-10 responses could be detected as a result of vaccination with both the MenBvac and the MeNZB vaccines given separately, as well as when given in combination. With respect to cross-reactivity, the cytokine results paralleled the observations made for proliferation. In conclusion, the results demonstrate that cross-reactive cellular immune responses involving both Th1 and Th2 cytokines can be induced to the same extent by different tailor-made OMV vaccines given either separately or in combination with half the dose of each vaccine.
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Conrad, Marcel E., Jay N. Umbreit, Elizabeth G. Moore, Lucille N. Hainsworth, Michael Porubcin, Marcia J. Simovich, Marian T. Nakada, Kevin Dolan, and Michael D. Garrick. "Separate pathways for cellular uptake of ferric and ferrous iron." American Journal of Physiology-Gastrointestinal and Liver Physiology 279, no. 4 (October 1, 2000): G767—G774. http://dx.doi.org/10.1152/ajpgi.2000.279.4.g767.

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Separate pathways for transport of nontransferrin ferric and ferrous iron into tissue cultured cells were demonstrated. Neither the ferric nor ferrous pathway was shared with either zinc or copper. Manganese shared the ferrous pathway but had no effect on cellular uptake of ferric iron. We postulate that ferric iron was transported into cells via β3-integrin and mobilferrin (IMP), whereas ferrous iron uptake was facilitated by divalent metal transporter-1 (DMT-1; Nramp-2). These conclusions were documented by competitive inhibition studies, utilization of a β3-integrin antibody that blocked uptake of ferric but not ferrous iron, development of an anti-DMT-1 antibody that blocked ferrous iron and manganese uptake but not ferric iron, transfection of DMT-1 DNA into tissue culture cells that showed enhanced uptake of ferrous iron and manganese but neither ferric iron nor zinc, hepatic metal concentrations in mk mice showing decreased iron and manganese but not zinc or copper, and data showing that the addition of reducing agents to tissue culture media altered iron binding to proteins of the IMP and DMT-1 pathways. Although these experiments show ferric and ferrous iron can enter cells via different pathways, they do not indicate which pathway is dominant in humans.
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Dissertations / Theses on the topic "Separateur de cellule"

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BONTE, PIERRE-HENRY. "Obtention simultanee de concentre plaquettaire standard et de concentre globulaire appauvri en leucocytes a l'aide de l'automate ex 30." Lille 2, 1991. http://www.theses.fr/1991LIL2M170.

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GASPARD, MARIE. "Conditions de realisation de 222 lymphaphereses chez 32 adultes et 9 enfants lors de traitement par interleukine 2 in vivo et cellules lak." Lyon 1, 1989. http://www.theses.fr/1989LYO1M435.

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Lee, Peter L. "Adipocyte mTORC1 Signaling Separately Regulates Metabolic Homeostasis and Adipose Tissue Mass, Independent of RagGTPase Activity." eScholarship@UMMS, 2018. https://escholarship.umassmed.edu/gsbs_diss/988.

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Metabolic disorders are commonly associated with obesity, a condition where excess caloric intake leads to massive adipose tissue (AT) expansion and eventual dysfunction. When adipose tissue loses its ability to store excess energy properly, lipids accumulate in non-adipose tissues such as liver, and muscle. This ectopic lipid deposition is a significant risk factor in the development of a collection of disorders described as metabolic syndrome. While metabolic syndrome is typically linked with obesity, patients who have an inability to develop adipose tissue depots (lipodystrophy) develop similar clinical outcomes. There is evidence that aberrant mTORC1 signaling may occur in both settings, and may be a factor that contributes to adipose dysfunction. I find that adipocyte specific loss of Raptor, a key mTORC1 subunit, leads to progressive lipoatrophy, and associated metabolic dysfunction including AT inflammation, hepatosteatosis, and insulin resistance. Interestingly, inhibition of autophagy, a pathway upregulated during Raptordeletion, prevents lipoatrophy but does not protect from ectopic lipid deposition and AT inflammation. These results suggest that outputs of mTORC1 in adipocytes individually regulate adipocyte storage capacity, and AT health. Furthermore, ablation of the amino acid sensing RagGTPases, thought to be necessary for mTORC1 activity, does not phenocopy Raptor KO, suggesting RagGTPase independent functions of mTORC1 in adipocytes. RagA/B deletion, however, did consistently increase Ucp1 expression in WAT, indicating a possible noncanonical role of the Rags in regulating Ucp1. Overall, these studies advance our understanding of regulation of adipose tissue metabolism, and shed light on previously unstudied nutrient specific signaling pathways in adipocytes.
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Disotell, Kevin James. "Low-Frequency Flow Oscillations on Stalled Wings Exhibiting Cellular Separation Topology." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1449162356.

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Tomečková, Andrea. "Využití Kluyveromyces marxianus k produkci bioethanolu z odpadního papíru." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2014. http://www.nusl.cz/ntk/nusl-217086.

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The diploma thesis is focused on production possibilities of bioethanol from waste paper by yeast Kluyveromyces marxianus. Waste cardboard was used as a potential substrate for bioethanol production. Several methods for cardboard preparation were introduced and compared as well as methods of fermentation. Simultaneous sacharification and fermentation and separate hydrolysis and fermentation of preprepared cardboard paper were performed in different pH buffer (4,8-7). Simultaneous sacharification and fermentation was held at a temperature of 45°C. Hydrolysis in separate hydrolysis and fermentation was performed at 50°C and fermentation at 25°C. Procedures outputs were obtained by sampling in specific time intervals and samples were analyzed by HPLC for presence and concentration glucose and ethanol. The results of the analysis have shown that the highest concentration of glucose produced by enzymatic hydrolysis was achieved by using microwaves, 2% H2SO4 and 2% NaOH pretreated paperboard at pH 4,8. The highest yield of ethanol was obtained by separate hydrolysis and fermentation of pulp pretreated by microwaves, 2% H2SO4 and 2% NaOH in pH 5,4 buffer. The method SHF proved to be more effective for the production of ethanol than SSF.
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Lin, Pei-Ying, and 林佩穎. "Modification of Bacterial Cellulose Membrane as a Separator for Lithium Ion Batteries." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/02450058122128993393.

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碩士
國立雲林科技大學
化學工程與材料工程系碩士班
101
This study attempts to propose an effective method in order to eliminate the troublesome hydroxyl groups of bacterial cellulose (BC) membrane while preserving its microfibrillar morphology for its possible application in lithium-ion battery (LIB). A fibrous acetylation method is adopted to modify BC membrane for this purpose. Furthermore, it is found that different drying methods can also lead to different morphology and have a profound influence on the physical properties of the BC separator. Under fixed compositions but different reaction durations for acetylation treatment, it is observed that acetylation reaction for 60 min (i.e. corresponding to a degree of substitution, DS = 1.65) followed by freeze drying is an optimum condition to modify a BC membrane. The corresponding membrane, referred as FA60BC, exhibits excellent properties in thermal stability (nearly zero shrinkage under 150 ℃ for 30 min), electrolyte uptake (up to 279 %), and wettability for liquid electrolyte solution. The ionic conductivity of the FA60BC membrane was 0.58 mScm-1, close to the value of 0.64 mScm-1 of the commercial Celgard 2325 membrane. A lithium-ion cell assembled with the FA60BC separator showed stable cycling performance and high rate capability. Linear sweep voltammetry (LSV) test indicates the FA60BCseparator is even superior to Celgard 2325 in electrochemical stability window. This preliminary study indicates BC membrane is very promising to act as a separator in LIB.
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Books on the topic "Separateur de cellule"

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Thomson, Robert G., and Peter Abramoff. Cellular Reproduction: Separate from Laboratory Outlines in Biology VI. W. H. Freeman, 1995.

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Thomson, Robert G., and Peter Abramoff. Cellular Respiration: Separate from Laboratory Outlines in Biology VI. W. H. Freeman, 1995.

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Pivec, Tanja, Tamilselvan Mohan, Rupert Kargl, Manja Kurečič, and Karin Stana Kleinschek. Design, Characterisation and Applications of Cellulose-Based Thin Films, Nanofibers and 3D Printed Structures: A Laboratory Manual. University of Maribor Press, 2021. http://dx.doi.org/10.18690/978-961-286-432-3.

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The introduction of the Laboratory Manual gives the theoretical bases on cellulose and its derivatives, which are used as starting polymers for the preparation of multifunctional polymers with three different advanced techniques - spin coating, electrospinning and 3D printing. In the following, each technique is presented in a separate Lab Exercise. Each exercise covers the theoretical basics on techniques for polymer processing and methods for their characterisation, with an emphasis on the application of prepared materials. The experimental sections contain all the necessary information needed to implement the exercises, while the added results provide students with the help to implement correct and successful exercises and interpret the results.
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Book chapters on the topic "Separateur de cellule"

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Edwards, N. Lawrence, Annette M. Zaytoun, and Gail A. Renard. "Separate Mechanisms for Cellular uptake of Purine Nucleotides by B- and T-Lymphoblasts." In Purine and Pyrimidine Metabolism in Man V, 463–65. New York, NY: Springer US, 1986. http://dx.doi.org/10.1007/978-1-4684-1248-2_72.

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Ashby, Barrie. "Computer Simulation of Prostaglandin-Regulated Cyclic AMP Metabolism in Platelets: Activation and Desensitization of Adenylate Cyclase Appear to be Mediated Through Separate Prostaglandin Receptors Coupled to GS and Gi." In Biology of Cellular Transducing Signals, 73–82. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4613-0559-0_8.

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Warheit, D. B., M. A. Hartsky, S. R. Frame, and C. J. Butterick. "Comparisons of Pulmonary Effects in Rats Exposed to Size-Separated Preparations of Para-Aramid Or Chrysotile Asbestos Fibres After 2-Week Inhalation Exposures." In Cellular and Molecular Effects of Mineral and Synthetic Dusts and Fibres, 285–98. Berlin, Heidelberg: Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-642-79041-6_28.

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Bołt, Witold, Barbara Wolnik, Jan M. Baetens, and Bernard De Baets. "On the Identification of $$\alpha $$ α -Asynchronous Cellular Automata in the Case of Partial Observations with Spatially Separated Gaps." In Challenging Problems and Solutions in Intelligent Systems, 23–36. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-30165-5_2.

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Magri, Mara. "Writing a Quality Management Plan." In Quality Management and Accreditation in Hematopoietic Stem Cell Transplantation and Cellular Therapy, 107–21. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-64492-5_13.

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AbstractQuality management systems (QMS) are central to FACT-JACIE accreditation standards for haematopoietic cell therapy (HCT) programmes. The quality management plan (QMP) defines the acceptable level of quality of the activities performed or of the products released by the HCT programme and describes how this level of quality in its deliverables and work processes will be ensured. An integrated HCT programme may have a single or several QMPs that addresses all aspects of the clinical, collection, and processing facilities, whilst separate organizations providing the components of an HCT programme may have distinct QMPs joined via service-level agreements (SLAs) and other arrangements. The QMP or QMPs must detail all key elements that affect the quality of recipient and donor care and cellular therapy products. The specific SOPs to be followed for each of these elements should be referenced within QMPs, and linked to the appropriate document where the details are described. The thoroughness in preparation of a QMP and its implementation reflect how quality is perceived within the HCT programme and by external inspectors in the accreditation process.
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Saltzman, W. Mark. "Tissue Barriers to Molecular and Cellular Transport." In Tissue Engineering. Oxford University Press, 2004. http://dx.doi.org/10.1093/oso/9780195141306.003.0014.

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Previous chapters have revealed the importance of molecular diffusion in tissue engineering. Molecules—and gradients of molecules—may represent the underlying mechanism of tissue induction and pattern formation (Chapter 3); growth factors—and the rate of delivery of growth factors to a cell surface—can influence the rate of cell proliferation (Chapter 4); chemoattractants can influence the rate and pattern of cell migration within a tissue space (Chapter 7). To think quantitatively about these processes, it is often helpful to think about molecular concentrations and the spatial variations in concentration that produce diffusion fluxes. This idea has been illustrated earlier in the book for specific examples such as bicoid gradient formation in the insect embryo (Section 3.3.4) and ligand diffusion to the cell surface (Section 4.3.2). Some of the basic concepts of molecular transport are also reviewed in Appendix B. But tissues are often heterogeneous structures, formed by the assembly of cells and the accumulation of matrix materials in the extracellular space. The heterogeneous composition of tissues can have a dramatic influence on local rates of molecular movement through the tissue; capillary endothelial cells prevent the diffusion of intravascular proteins into the tissue interstitial space, for example. This chapter discusses this concept and provides quantitative methods for evaluating rates of molecular movement between tissue spaces that are separated by diffusive barriers. In addition, the last section of the chapter shows how this same analysis may be useful when thinking about rates of cellular movement between tissue compartments. In multicellular organisms, thin lipid membranes serve as semipermeable barriers between aqueous compartments. The plasma membrane of the cell separates the cytoplasm from the extracellular space; endothelial cell membranes separate the blood within the vascular space from the rest of the tissue. Properties of the lipid membrane are critically important in regulating the movement of molecules between aqueous spaces. While certain barrier properties of membranes can be attributed to the lipid components, accessory molecules within the cell membrane—particularly transport proteins and ion channels—control the rate of permeation of many solutes.
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"Processing and Recognition of Images Based on Asynchronous Cellular Automata." In Advances in Computer and Electrical Engineering, 194–242. IGI Global, 2021. http://dx.doi.org/10.4018/978-1-7998-2649-1.ch007.

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This chapter discusses the use of asynchronous cellular automata with controlled movement of active cells for image processing and recognition. A time-pulsed image description method is described. Various models and structures of cellular automata for transmitting active signals are presented. The image of the figure is binarized and an active signal moves along its edges. At every moment in time, the active cell of an asynchronous cellular automaton generates a pulse signal. The shape of the generated pulse sequence describes the geometric shape of a flat figure. Methods for describing images of individual plane figures, as well as a method for describing images consisting of many separate geometric objects, are proposed. Cellular automaton is considered as an analogue of the retina of the human visual canal. The circuitry structures of cells of such asynchronous cellular automata are presented, and the software implementation of the proposed methods is also performed. Methods allow one to classify individual geometric image objects.
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Kari, Lila, and Laura F. Landweber. "Biocomputation in Ciliates." In Cellular Computing. Oxford University Press, 2004. http://dx.doi.org/10.1093/oso/9780195155396.003.0016.

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Ciliates are unicellular protists that may have arisen more than a billion years ago. They have since diverged into thousands of species, many uncharacterized, the genetic divergence among ciliates being at least as deep as that between plants and animals. Despite their diversity, ciliates are united by two common features; the presence of short threads called cilia on their surface, whose rhythmic beating causes movement and is also useful for food capture, and the presence of two types of nuclei. The macronucleus contains DNA encoding functional copies of all the genes that regulate vegetative growth and cell proliferation. The micronucleus contains encrypted versions of the macronuclear DNA, is mostly functionally inert, and is only used for sexual exchange of DNA. In this chapter we study the decryption of the macronuclear DNA from a computational perspective. When two cells mate, they exchange micronuclear information. After they separate, the old micronuclei and macronuclei degenerate, while the newly formed micronuclei develop into new macronuclei over hours or days, depending on the species. Few ciliates have so far been studied at the level of molecular genetics: Tetrahymena and Paramecium representing the Oligohymenophorans and Oxytricha (recently renamed Sterkiella), and Stylonichia and Euplotes representing Spirotrichs. The DNA molecule in each of the approximately 120 chromosomes in the micronucleus contains on average approximately 107 basepairs (bp) in Oxytricha species and approximately 18 × 106 bp in Stylonichia lemn. The size of the DNA molecules in the macronucleus is, in contrast, very small. In various Oxytricha species and S. lemnae, macronuclear DNA molecules range in size from 400 to 15,000 bp with most molecules in the 1000–8000 bp range. Macronuclear DNA sequences are derived from the micronuclear sequences through a series of DNA rearrangements as follows. The segments that together constitute a macronuclear sequence (macronuclear destined sequences or MDSs) are present as sub-sequences in the micronuclear DNA. However, in the micronuclear DNA, MDSs are interspersed with long DNA sequences (internal eliminated sequences or IESs) that are excised in the micronucleus to macronucleus differentiation.
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Das, Amit, Rakhi Dasgupta, and Angshuman Bagchi. "Overview of Cellular Computing-Basic Principles and Applications." In Handbook of Research on Natural Computing for Optimization Problems, 637–62. IGI Global, 2016. http://dx.doi.org/10.4018/978-1-5225-0058-2.ch026.

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Computers, due to their raw speed and massive computing power, have been highly used by biologists to expedite life science research whereas several computational algorithms like artificial neural network, genetic algorithm and many similar ones have been inspired by the behaviors of several biological or cellular entities. However till date both these disciplines i.e. life sciences and computer sciences have mostly progressed separately while recent studies are increasingly highlighting the impact of each discipline on the other. The chapter describes several features of biological systems which could be used for further optimizations of computer programs or could be engineered to harness necessary computational capabilities in lieu of traditional silico chip systems. We also highlight underlying challenges and avenues of implementations of cellular computing.
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Das, Amit, Rakhi Dasgupta, and Angshuman Bagchi. "Overview of Cellular Computing-Basic Principles and Applications." In Data Analytics in Medicine, 1895–920. IGI Global, 2020. http://dx.doi.org/10.4018/978-1-7998-1204-3.ch095.

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Computers, due to their raw speed and massive computing power, have been highly used by biologists to expedite life science research whereas several computational algorithms like artificial neural network, genetic algorithm and many similar ones have been inspired by the behaviors of several biological or cellular entities. However till date both these disciplines i.e. life sciences and computer sciences have mostly progressed separately while recent studies are increasingly highlighting the impact of each discipline on the other. The chapter describes several features of biological systems which could be used for further optimizations of computer programs or could be engineered to harness necessary computational capabilities in lieu of traditional silico chip systems. We also highlight underlying challenges and avenues of implementations of cellular computing.
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Conference papers on the topic "Separateur de cellule"

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Brewer, Bryson M., Mingjian Shi, Yandong Gao, Donna J. Webb, Jon F. Edd, and Deyu Li. "Microfluidic Cell Co-Culture Platform With Liquid Fluorocarbon as the Cell Separator." In ASME 2012 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/imece2012-87601.

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Cell co-culture platforms are essential tools for investigating inter-cellular communication and cellular activities among different cell populations. Microfluidic cell co-culture platforms offer several advantages over their conventional counterparts, including precise control over the cellular microenvironment, low cost, and high throughput. Previously, we have developed microfluidic devices using a hydraulically/pneumatically controlled polydimethylsiloxane (PDMS) valve barrier to separate distinct cell populations in culture, providing a means for manipulation and specific treatment of each cell type with different reagents [1]. After releasing the barrier, different cell populations can interact with each other while being observed using real-time imaging. However, the solid PDMS valve barrier is not truly reversible, as any cells/cell processes underneath the barrier will likely undergo physical damage when the valve barrier is activated.
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Khafagy, Mohammad, Amr El-Keyi, Mohammed Nafie, and Tamer ElBatt. "Degrees of freedom for separated and non-separated half-duplex cellular MIMO two-way relay channels." In ICC 2012 - 2012 IEEE International Conference on Communications. IEEE, 2012. http://dx.doi.org/10.1109/icc.2012.6364553.

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Wildman, Jeffrey, Yusuf Osmanlioglu, Steven Weber, and Ali Shokoufandeh. "Delay minimizing user association in cellular networks via hierarchically well-separated trees." In 2015 IEEE International Conference on Signal Processing for Communications (ICC). IEEE, 2015. http://dx.doi.org/10.1109/icc.2015.7248950.

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Zhao, De, Wei Wang, Zhibin Li, Kang Li, and Ruoyun Chen. "Modeling the Bicycle Passing Events on Physically Separated Bicycle Roadway Using Cellular Automaton." In 11th International Conference of Chinese Transportation Professionals (ICCTP). Reston, VA: American Society of Civil Engineers, 2011. http://dx.doi.org/10.1061/41186(421)65.

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Purwanti, Endah, Winda Trisna Wulandari, Achmad Rochliadi, Bunbun Bundjali, and I. Made Arcana. "Preparation of nanocrystalline cellulose from corncob used as reinforcement in separator for lithium ion battery." In 2015 Joint International Conference on Electric Vehicular Technology and Industrial, Mechanical, Electrical and Chemical Engineering (ICEVT & IMECE). IEEE, 2015. http://dx.doi.org/10.1109/icevtimece.2015.7496689.

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An, Seungdo, Kyung Soo Kim, Ji-Man Cho, HoJoon Park, and Ghun Hahm. "A Micromachined Magnetometer-Accelerometer." In ASME 2002 International Mechanical Engineering Congress and Exposition. ASMEDC, 2002. http://dx.doi.org/10.1115/imece2002-33304.

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A new magnetometer-accelerometer is developed with the silicon micromachining. The application areas include the cellular phones providing the third generation (3G) mobile service, and 3D game devices. The structure with a current-flowing conductor changes the vibration amplitude due to the Lorentz force. Both the magnetic field and acceleration are detected from the capacitance change and separated into each signal by the modulated frequency difference in one microstructure. A new Samsung MEMS fabrication process is developed for this sensor. The process uses a silicon-on-glass (SOG) wafer, an inverted SOG wafer, and a gold-silicon eutectic bonding for the wafer-level hermetic packaging. The test shows the equivalent noise of 1.65 mg peak-to-peak (pp) acceleration and 70 μT pp magnetic flux density. The sensor performance is enough for the 10 degree electronic display of the orientation angle for the cellular phones and portable navigators.
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Schick, K. P., S. Shapiro, G. Tuszynski, and J. Slawek. "SULFATIDES AND GLYCOLIPIDS IN PLATELETS AND ENDOTHELIAL CELLS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643641.

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Sulfatides are sulfated glycolipids which are negatively charged and thought to influence receptor mediated activities. Sulfatides have the capacity to provide a surface for the initiation of in vitro coagulation tests and these acidic lipids represent the potential biological surface for the initiation of the contact and intrinsic systems in vivo. Several sulfatides have been demonstrated in blood platelets. We have investigated sulfatides and other glycolipids in endothelial cells and platelets in order to define the cellular sources for sulfatides that would be available for influencing hemostasis. Endothelial cells were derived from primary cultures of human umbilical veins and human platelets were obtained from freshly-collected blood. Cellular lipids were extracted by the Folch method. Sulfatides and glycolipids were purified by silicic acid chromatography, separated by thin-layer chromatography, and quantitated by the assay of sphingosine. Glycolipids were also analyzed by HPLC. Globoside was found to be the predominant glycolipid in endothelial cells while lactosyl ceramide was the predominant glyco-lipid in platelets. Sulfatides were detected by two approaches: 1) Sulfatide synthesis by the incorporation of [35S]-Sulfate; 2) The specific binding of [125I]-thrombospondin and [125I]-von Willebrand’s factor (vWF) to sulfatides separated by thin-layer chromatography (TLC). Several sulfatides were identified in endothelial cells and platelets by virtue of the incorporation of [35S]-sulfate into glycolipids separated by TLC. [125I]-TSP and [125I]-vWF bound to the glycolipids that had incorporated [35S]-sulfate. [35S]-sulfate was primarily incorporated into sulfated galactosyl ceramide but both cells also synthesized complex glycolipids. TSP and vWF were shown to bind to sulfated galactosyl ceramide, a band that comigrated with glycosyl ceramide as well as with two more complex sulfatides in both cells. However, differences in sulfatide synthesis and binding of TSP to sulfatides were observed in endothelial cells from that in platelets. The study indicates that endothelial cells and platelets contain several sulfatides and thus are potential sources for sulfatides for the initiation of coagulation.
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David, Regis A., Justin L. Black, Brian D. Jensen, and Sandra H. Burnett. "Modeling and Experimental Validation of DNA Motion During Electrophoresis." In ASME 2010 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2010. http://dx.doi.org/10.1115/detc2010-28541.

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This paper describes the protocol and presents the results for DNA motion experiments using fabricated macroscale gel electrophoresis devices. Gel electrophoresis is a process used to separate/move DNA, RNA or protein molecules using an electric field through a gel matrix (electrolytic solution). In electrolytic solutions, the current conduction is due to a transport of ions (anions and cations). A better understanding of electrophoretic fundamentals allows for modeling the motion of DNA during electrophoresis. The model is validated through comparison with the experimental results. The model and experimental validation will be used to improve the process of cellular nanoinjection of DNA, currently in development in our lab.
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Pidard, D., A. Fischer, C. Bouillot, F. Ledeist, and A. T. Nurden. "INHERITED DEFICIENCIESCAN AFFECT SEPARATELY THE PLATELET MEMBRANE GLYCOPROTEIN Ilb-IIIa COMPLEX AND THE LEUKOCYTE LFA-1, Mac-1 and pl50,95 COMPLEXES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643704.

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The human platelet membrane glycoprotein (GP) IIb-IIIa complex and a family of functional leukocyte cell membrane antigens, LFA-1 (L), Mac-1 (M) andpl50,95 (X), possess knownstructural analogies. Similaritiesinclude a heterodimeric structure with a high mol. wt. αsubunit (Mr∽ 145-180 kDa) , associated nonconvalently with a lower mol. wt.β-subunit (Mr ∽ 90-95 kDa),anda partial amino acid sequence hommology between GP lib and αL or CKM. Furthermore, GP lib, α L and αM were reported to be co-expressed in murine cells transfected with a 20 kilobase human DNA fragment.To address the question of a possible genomic linkage between these related glycoproteins of different cells, we have examined patients with either ‘LFA-1 immunodeficiency disease’ or with Glanzmann's thrombasthenia (GT). S.Bo. and P.Ce. are children affected by recurrent bacterial infections since birth. Cytofluorimetric analysis using monoclonal antibodies (MAbs) for the αL, αM,αX and β subunits demonstrated that the surface expression of LFA-1, Mac-1 and pl50,95 intheir leukocytes was ≤1% of the normal values. Platelets from both patients aggregated normally and readily bound the MAbs AP-2 (anti-GP IIb-IIIa) or Tab (anti-GP IIb). Crossed immunoelectrophoresis confirmed the presenceof GP IIb-IIIa complexes, whileSDS-polyacrylamide gel electrophoresis showed a normal migration of GP IIb and GP IIIa. Patients C. Bl. and M.Ca.are typical of the type I subgroupofGT. Their platelets are unable toaggregate and contain < 1% of the normal platelet content of GP liband GP Ilia.Cytofluoro-graphy showed a normal expression of LFA-1, Mac-1 and 150,95 on the surface of lymphocytes, polymorphonuclear cells and/or monocytes of both patients. Our data thus show that despite sequence homologies and a potential genomic linkage, the cellular expression of the platelet GP IIb-IIIa complex and of the LFA-1 related leukocyte antigens may be dissociated in genetic disorders where anabnormal glycoprotein distribution may be restricted to one type of cell.
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Barrera, C., A. Arrieta, and N. Escobar. "Application of Conducting Polymer Composites With Cellulose Fibers on Water Softening." In ASME 2012 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/imece2012-89969.

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Hard water is unsuitable for industrial and domestic purposes given its high levels of calcium and magnesium divalents which generate scale, oxidation and are antagonistic of optimal performance of detergents and industrial equipment. Conventional methods for water softening generate by-products that need to be treated, which makes these methods economically and environmentally unsustainable and open the opportunity to develop new technology for this application. The ion exchange behavior during the charge and discharge processes (i.e. oxidation / reduction), of conducting polymers and the combination of these materials with other such as fibers, to develop new hybrid materials that exhibit the inherent properties of both components, has been the object of many studies in the last years. The aim of this study is to evaluate the applicability of vegetable cellulose microfibers as a base to obtain a conducting polymer composite membrane with polypyrrole and to analyze the membrane performance to remove ions dissolved in hard water. The application of conducting polymer composite on water softening is based on the use of pyrrole’s electrochemical properties jointed to the flexibility and relatively high surface areas associated with cellulose, to promote an ion exchange reaction between the composite membrane and the hard water. The cellulose membranes obtained from banana plant waste (raquis), were uniform with individual and well separated fibers. The fibers were successfully encapsulated by a continuous coating of polypyrrole through in situ oxidative chemical polymerization. The amount of polypyrrole deposited on the fiber increased with increasing concentrations of the monomer, which was easily identified through the observation of differences on the intensity of the light to dark colour shift that coated the fibers after the polymerization. The applicability of the conducting polymer composite on water softening was tested using an experimental device, finding reductions on the conductivity for hard water within 23 to 66 μs/cm after 6 hours of the assay.
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Reports on the topic "Separateur de cellule"

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Dale, M. C., and F. Hart. A low energy continuous reactor separator for the production of ethanol from starch, molasses and cellulose. Fifth quarterly report, March 16--June 15, 1995. Office of Scientific and Technical Information (OSTI), September 1995. http://dx.doi.org/10.2172/106722.

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A low energy continuous reactor separator for the production of ethanol from starch, molasses and cellulose. Fourth quarterly report to the Energy Related Inventions Program, January 16--March 15, 1995. Office of Scientific and Technical Information (OSTI), June 1995. http://dx.doi.org/10.2172/101061.

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