Academic literature on the topic 'Site directed mutagensis'

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Journal articles on the topic "Site directed mutagensis"

1

Gudynaite-Savitch, Loreta, Christelle Loiselay, Leonid V. Savitch, et al. "The small domain of cytochromeffrom the psychrophileChlamydomonas raudensisUWO 241 modulates the apparent molecular mass and decreases the accumulation of cytochromefin the mesophileChlamydomonas reinhardtii." Biochemistry and Cell Biology 85, no. 5 (2007): 616–27. http://dx.doi.org/10.1139/o07-066.

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Cytochrome f from the psychrophile Chlamydomonas raudensis UWO 241 has a lower thermostability of its c-type heme and an apparent molecular mass that is 7 kDa lower than that of the model mesophilic green alga Chlamydomonas reinhardtii. We combined chloroplast transformation, site-directed mutagensis, and the creation of chimeric fusion constructs to assess the contribution of specific domains and (or) amino acids residues to the structure, stability, and accumulation of cytochrome f, as well as its function in photosynthetic intersystem electron transport. We demonstrate that differences in t
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2

de Vries, G. E. "Site-directed mutagenesis." Trends in Plant Science 5, no. 7 (2000): 276. http://dx.doi.org/10.1016/s1360-1385(00)01699-x.

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3

Carter, P. "Site-directed mutagenesis." Biochemical Journal 237, no. 1 (1986): 1–7. http://dx.doi.org/10.1042/bj2370001.

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4

Nybo, Kristie. "Site-directed mutagenesis: colony growth." BioTechniques 50, no. 2 (2011): 87–89. http://dx.doi.org/10.2144/000113609.

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5

Weiner, M. P., and G. L. Costa. "Rapid PCR site-directed mutagenesis." Genome Research 4, no. 3 (1994): S131—S136. http://dx.doi.org/10.1101/gr.4.3.s131.

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MacDonald, Nicholas J., José M. P. Freije, Mary L. Stracke, Richard E. Manrow, and Patricia S. Steeg. "Site-directed Mutagenesis ofnm23-H1." Journal of Biological Chemistry 271, no. 41 (1996): 25107–16. http://dx.doi.org/10.1074/jbc.271.41.25107.

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7

Vallone, B., P. Vecchini, V. Cavalli, and M. Brunori. "Site-directed mutagenesis in hemoglobin." FEBS Letters 324, no. 2 (1993): 117–22. http://dx.doi.org/10.1016/0014-5793(93)81375-a.

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8

Carey, Michael F., Craig L. Peterson, and Stephen T. Smale. "PCR-Mediated Site-Directed Mutagenesis." Cold Spring Harbor Protocols 2013, no. 8 (2013): pdb.prot076505. http://dx.doi.org/10.1101/pdb.prot076505.

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9

Costa, Gina L., and Michael P. Weiner. "Rapid PCR Site-Directed Mutagenesis." Cold Spring Harbor Protocols 2006, no. 1 (2006): pdb.prot4144. http://dx.doi.org/10.1101/pdb.prot4144.

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10

Erb, Laurie, Richard Garrad, Yanjun Wang, Tom Quinn, John T. Turner, and Gary A. Weisman. "Site-directed Mutagenesis of P2UPurinoceptors." Journal of Biological Chemistry 270, no. 9 (1995): 4185–88. http://dx.doi.org/10.1074/jbc.270.9.4185.

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