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Tesi sul tema "Molekulární biologie"

Autore: Grafiati
Pubblicato: 4 giugno 2021
Ultima modifica: 13 febbraio 2022

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1

Gajdová, Barbora. "Identifikace PHA produkujících bakterií pomocí nástrojů molekulární biologie." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2017. http://www.nusl.cz/ntk/nusl-316230.

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Abstract (sommario):
This diploma thesis deals with identification of bacteria which are capable of producing polyhydroxyalkanoates (PHAs). Work included testing variety of genera including Pseudomonas, Lactobacillus, Bifidobacterium, thermophilic cultures and samples gathered from natural sources. Bacteria were investigated by molecular technique polymerase chain reaction – PCR. An amplification of the PHA synthase gene (phaC) was analyzed. In the first reaction phaC and 16S rRNA genes were tested at the same time. 16S rRNA gene is used as control for bacterial DNA and as an identification tool for natural source
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2

Tomíšek, Martin. "Charakterizace rostlinné složky vybraných potravin pomocí technik molekulární biologie a instrumentálních metod." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2020. http://www.nusl.cz/ntk/nusl-432719.

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Abstract (sommario):
The aim of this work was to compare authenticity analysis in selected food products with fruit component by using, instrumental and molekular methods. Particularly, the presence of blueberries in fruit–based foodstuffs was verified. The theoretical part is focused on the characterization, chemical composition and botanical classification of blueberries (European blueberry and Canadian blueberry). It also contains an overview of instrumental and molecular diagnostic methods that can be used for the analysis of these fruits. The experimental part focuses on the selection of a suitable method of
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3

Drlík, Radovan. "Databázový systém pro správu biologických dat." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2010. http://www.nusl.cz/ntk/nusl-237252.

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Abstract (sommario):
This thesis describes the problems of storage and management of biological data, particularly of Haloalkane Dehalogenase enzymes. Furthermore, the thesis aims at project HADES (HAloalkane DEhalogenase databaSe) initiated by protein engineering group of Loschmidt Laboratories, Masaryk University in Brno. This is a project whose main goal is simply to store, preserve and display a wide variety of proteins data. The result of this work is a flexible database system allowing easy extensibility and maintainability, which is built on technologies Apache, PostgreSQL and PHP using the Zend Framework.
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4

Sladký, Roman. "Techniky pro porovnávání biologických sekvencí." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2008. http://www.nusl.cz/ntk/nusl-235890.

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Abstract (sommario):
This work presents the building up of basic biological units DNA, RNA and proteins as well as their function. Provided data are kept in biological databases which are connected worldwide to supply preferable communication along with all kinds of available information to be used in the scientific research. The secret of alive is hidden in genes coded in sequences of nucleotides. Genes enable the creation of proteins which are made of sequences of amino-acids. The wide-spread methods of comparing these sequences are FASTA and BLAST algorithms. Their base is used for the PSProt program which is d
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5

Žigárdi, Tomáš. "Klasifikace malých nekódujících RNA." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2015. http://www.nusl.cz/ntk/nusl-234981.

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Abstract (sommario):
This masters's thesis contains description of designed and implemented tool for classification of plant microRNA without genome. Properties of mature and star sequences in microRNA duplexes are used. Implemented method is based on clustering of RNA sequences (with CD-HIT) to mainly reduce their count. Selected representants from each clusters are classified using support vector machine. Performance of classification is more than 96% (based on cross-validation method using the training data).
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6

Jurásek, Petr. "Shlukování proteinových sekvencí na základě podobnosti primární struktury." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2009. http://www.nusl.cz/ntk/nusl-236761.

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Abstract (sommario):
This master's thesis consider clustering of protein sequences based on primary structure of proteins. Studies the protein sequences from they primary structure. Describes methods for similarities in the amino acid sequences of proteins, cluster analysis and clustering algorithms. This thesis presents concept of distance function based on similarity of protein sequences and implements clustering algorithms ANGES, k-means, k-medoids in Python programming language.
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7

Trávníček, Ivan. "Molekulární biologie nádorů ledvin v klinickém využití." Doctoral thesis, 2017. http://www.nusl.cz/ntk/nusl-369100.

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Abstract (sommario):
Molecular biology of renal tumours in clinical use MUDr. Ivan Trávníček Summary Aim: To identify tissue biomarker that are predictive of the therapeutic effect of sunitinib in treatment of metastatic clear cell renal cell carcinoma (mCRCC). Material and methods: Our study included 39 patients with mCRCC; these were selected from 119 patients who received sunitinib in our hospital between the years 2005 - 2012 according to inclusion criteria of the study. Patients were stratified into two groups based on their response to sunitinib treatment; non-responders (progression), and responders (stable
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8

Kripnerová, Michaela. "Modelové systémy při studiu a výuce molekulární biologie." Master's thesis, 2019. http://www.nusl.cz/ntk/nusl-405136.

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Abstract (sommario):
Molecular biology and genetics are currently very fast developing disciplines. One of the possibilities how to increase the pupils' interest in studying of molecular biology and genetics, and to support their own curiosity is to use research-based teaching strategies. Although Czech Education tries to correspond with this progress, teaching of molecular biology and genetics is limited not only by financial and material limits but also by a lack of vocational education. The diploma thesis is divided into theoretical and practical part. The theoretical part deals with the position of molecular b
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9

Pinkr, Tomáš. "Základy molekulární biologie na základních školách pomocí hands-on aktivit." Master's thesis, 2019. http://www.nusl.cz/ntk/nusl-405181.

Testo completo
Abstract (sommario):
The aim of this study is to prepare and verify a molecular biological teaching cycle. This cycle is focused on hands-on activities, critical thinking and student's activity. A total of 99 pupils from two elementary schools (9th grade) and one multi-year grammar school (equivalent to primary schools) participated in the verification. They helped to create an attractive learning cycle from molecular biology, which can be used directly without major modifications and low cost.
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10

Blaha, Milan. "Metody molekulární a buněčné biologie ve výuce na středních školách." Master's thesis, 2012. http://www.nusl.cz/ntk/nusl-308234.

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Abstract (sommario):
Teaching of cell and molecular biology is often restricted by financial and material limitations as well as a lack of expertise. These problems may be overcome by cooperation with scientific institutions and universities. Some scientists teach select courses under the auspices of high school. Others allow students to fulfill internships at their workplaces. The first aim of this thesis is to describe the characteristics of summer school, wherein the participants are integral for providing feedback on what matters most to them. The second goal of the study is to perform four molecular biology l
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11

Vrba, Vladimír. "Uplatnění metod molekulární a buněčné biologie ve výzkumu prvoků Eimeria." Doctoral thesis, 2011. http://www.nusl.cz/ntk/nusl-312099.

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Abstract (sommario):
Eimeria is an apicomplexan parasite causing disease coccidiosis that is most prominent in poultry farming industry. This thesis is aimed to develop new molecular tools and resolve issues that would be a valuable contribution in the field from both research and industry perspective. Because immunity to Eimeria is strictly species- specific, it is important to know and recognize correctly all species that parasitize the host. Traditional diagnostic approaches rely on classical methods such as oocyst morphology determination under the microscope, measurement of prepatent period or in-vivo assessm
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12

Žifčáková, Lucia. "Molekulární biologie a ekologie rozkladu rostlinných biopolymerů v lesních ekosystémech mikroorganismy." Doctoral thesis, 2017. http://www.nusl.cz/ntk/nusl-293536.

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Abstract (sommario):
The abilities of fungi and bacteria to degrade simple and complex carbon compounds derived from different sources, such as root exudates, litter, soil organic matter or fungal mycelium were studied in this dissertation. Knowledge of functional traits, especially degradation abilities of fungi and bacteria, are important for deciphering the black box of microbial functioning in topsoil and thus aiding in modeling and predicting future directions of microbial communities development in face of global changes. Among fungal cultures form culture collection representing strains with different taxon
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13

Hanušová, Eva. "Výuka molekulární biologie na SŠ a analýza prekonceptů pojmu nukleové kyseliny." Master's thesis, 2016. http://www.nusl.cz/ntk/nusl-349411.

Testo completo
Abstract (sommario):
The theoretical part assembles methodological starting points for the research - the content and meaning of curricula documents for science education; the contemporary scientific knowledge about selected molecularly biological topics taught at secondary schools; the characteristics of student preconceptions and misconceptions, their diagnosis and importance. The practical part contains the setting of research problems and aims related to their object, presented as the content analysis of curriculum and the teaching methods concerning molecularly biological topics at a selected secondary school
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14

Voříšková, Jana. "Molekulární biologie půdních hub, podílejících se na rozkladu opadu v lesních ekosystémech." Doctoral thesis, 2013. http://www.nusl.cz/ntk/nusl-327218.

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Abstract (sommario):
In forest ecosystems, substantial part of carbon enters soil in the form of plant litter. The decomposition of litter and soil organic matter represents an important process affecting nutrient cycling and carbon balance in soils. Fungi are considered the primary decomposers in terrestrial ecosystems due to the production of wide range of extracellular enzymes that allow them to attack the lignocellulose matrix in litter. Even if fungi represent key players in organic matter decomposition, the information about the structure and diversity of their communities is still limited and the roles of i
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15

Hyliš, Miroslav. "Molekulární fylogeneze, biologie a klasifikace mikrosporidií podle modelových skupin parazitujících u Trichopter a Lepidopter." Doctoral thesis, 2007. http://www.nusl.cz/ntk/nusl-375031.

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Abstract (sommario):
RESULTS: Theresultsof thePh.D.thesiscanbesummarizedinto4 thematicareas: l ) screeningand experimentaldataconcemingthe prevalence,genusand speciesdiversíty. hostandtissuespeciťrcityand of ecological. pathofyziologicalcharactersof infectionsand of theirtransmission. 2) classificationandtaxonomystudies 3) resultsofsequencingfollowedby phylogenyanalysis 4) resultsoťcomparativestudybasedon summarizeddata Ad r) T\."enty-fourmicrosporidiafrom Trichopteraand Lepidopteraas well as 5 microsporidiafrom potentialintermediatehosts (Crustacea,Amphipoda) were isolatedand screenedusing light andelectronmicros
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16

HAJDUŠKOVÁ, Martina. "Kvantitativní detekce spirochet \kur{Borrelia burgdorferi} sensu lato v biologickém materiálu metodami molekulární biologie." Master's thesis, 2005. http://www.nusl.cz/ntk/nusl-43305.

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17

Vlasáková, Jana. "Metody molekulární biologie ve forenzní genetice jako téma odborné přednášky na SŠ gymnaziálního typu." Master's thesis, 2008. http://www.nusl.cz/ntk/nusl-288105.

Testo completo
Abstract (sommario):
The title of the thesis is "The methods of molecular biiology in the court genetics as a topic of a specialized lesson on a high school". The thesis consideres the problematics of molecular biology methods that are used by the court genetics. It describes the respective methods and techniques utilized in the criminalistic routine. The work is split into six main chapters. The first, introductive chapter includes hypothesis and sets down the goals if the thesis. The second part dwells on the methods of molecular biology. It mentions the base information on DNA, methods and technologies used in
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18

Sikora, Jakub. "Nové aspekty molekulární biologie a patologie lysosomálních střádacích onemocnění. Studie s částečným využitím Caenorhabditis elegans." Doctoral thesis, 2007. http://www.nusl.cz/ntk/nusl-271504.

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19

Janštová, Vanda. "Vliv praktické výuky na motivaci žáků středních škol ke studiu biologie." Doctoral thesis, 2016. http://www.nusl.cz/ntk/nusl-350119.

Testo completo
Abstract (sommario):
The reasons for the decline in students' interest in science have been studied for the last decades. The key question is how to stimulate this interest and motivate students and pupils to study science. Part of this thesis was testing the influence of practical courses on upper secondary school students' motivation toward studying biology. To start with, it was found out how many practical courses were taught for each biology topic at Czech upper secondary schools. Molecular biology and genetics emerged as one of the topics with the lowest number of practical courses. Therefore, molecular biol
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20

Mašínová, Tereza. "Ekologie kvasinek v lesních půdách." Doctoral thesis, 2017. http://www.nusl.cz/ntk/nusl-370864.

Testo completo
Abstract (sommario):
Microbial communities inhabiting upper soil horizons represent an important component of forest ecosystems. However, despite the evidence that yeasts represent an integral part of topsoil fungal communities, their role in forest ecosystems received so far little attention. The aims of my PhD thesis were to describe yeast communities in soil and litter of a temperate forest using high- throughput sequencing of environmental DNA, identify dominant yeast species and to explore how the composition of yeast communities reflects the biotic and abiotic factors of the environment. I also aimed to isol
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21

Krasnovský, Pavol. "Deterministické a stochastické modely v molekulární a buněčné biologii." Master's thesis, 2012. http://www.nusl.cz/ntk/nusl-305140.

Testo completo
Abstract (sommario):
This thesis presents the main methods that are used to model the time evolution of the number of molecules in a cell. Two of the main aims in cell biology are to compute first the transi- tion probability function and second the density of the invariant measure. These two problems imply a number of conditions and hence we also include the ergodic theory and theory of the invariant measure. We use two illustrative examples of the application of the previously mentioned theories. We verify the necessary and sufficient conditions for the computation of the transition probability function and the
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22

Fík, Zdeněk. "Nové trendy v buněčné a molekulární biologii karcinomů hlavy a krku." Doctoral thesis, 2014. http://www.nusl.cz/ntk/nusl-338497.

Testo completo
Abstract (sommario):
Head and neck squamous cell carcinomas are still challenging despite progress in the oncological treatment. Study of the molecular biology allows to deeply characterize tumor properties and to predict the prognosis for affected patients. Nowadays there are many drugs clinically tested in the group of targeted therapy medicine Experimental work comprised both in vitro and in situ assays, being performed thanks to the collaboration between a number of departments of the 1st Faculty of Medicine of the Charles University in Prague, Academy of Sciences of the Czech Republic, Institute of Hematology
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23

Parolek, Jan. "Evaluace vlastností polymerních konjugátů specificky vážících proteiny pro použití v molekulární biologii." Master's thesis, 2015. http://www.nusl.cz/ntk/nusl-331798.

Testo completo
Abstract (sommario):
During last three decades, a great effort was invested to the development of polymer conjugates of low molecular drugs with the aim to improve the specific targeting of drugs to diseased tissues, cells and organs. The main reason for this effort was the fact that high molecular weight copolymers have a favourite distribution profile in tissues and organisms. A linker between a polymer backbone and drug has very important role: it is possible to synthesize a biodegradable linker, which can be enzymatically hydrolyzed. Conversely, there is a possibility to synthesize an inert linker, resistant t
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24

Kastner, Jan. "Studium časné kancerogeneze u spinoceluárního karcinomu hlavy a krku." Doctoral thesis, 2020. http://www.nusl.cz/ntk/nusl-410158.

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Abstract (sommario):
Squamous cell carcinoma is the most frequent malignant tumour of head and neck. Prognostic and predictive information as an individual imprint of molecular-genetic analysis of HNSCC will help to determine the best indivicual treatment. And in case of surgical appraoch the optimal resection with adequate quality of life and long-term survival. Study of early cancerogenesis in our project is based on knowledge, that histological normal mucosa next to tumor shows preneoplastic molecular alterations. Molecular genetic changes in a histological normal mucosa harbouring a tumor may play a principal
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25

Janoušek, Josef. "Population biology of the pine needle pathogen Lecanosticta acicola (Thüm.) Syd. (Capnodiales, Ascomycota)." Doctoral thesis, 2015. http://www.nusl.cz/ntk/nusl-249316.

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Abstract (sommario):
Lecanosticta acicola is a heterothallic ascomycete that causes brown spot needle blight (BSNB) on native and non-native Pinus spp. in many regions of the world. The aim of this thesis was to elucidate the origin of L. acicola populations in Europe and consider the reproductive mode of the pathogen in affected areas. In order to study the population genetics of L. acicola, eleven polymorphic microsatellite markers were developed. In addition, mating type markers that amplify both mating type idiomorphs (MAT1-1 and MAT1-2) were designed and the protocols for their applications were optimised. Co
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