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Journal articles on the topic '16S Rdna Sequencing of P. Aeruginosa'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Iliyasu, M. Y., R. A. Bamanga, A. F. Umar, E. B. Agbo, and A. Uba. "16S rDNA Sequencing analysis in identification of some multidrug resistant (MDR) bacterial isolates from clinical specimens." Nigerian Journal of Biotechnology 36, no. 2 (2020): 158–66. http://dx.doi.org/10.4314/njb.v36i2.16.

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Accurate identification of bacterial pathogens from clinical specimens and Multidrug Resistant (MDR) characterization is a key to empirical therapy. Twelve (12) bacteria isolates from blood, urine and faecal samples were selected based on the ability to grow on Luria Bertani (LB) agar medium containing 100μg/ml ampicillin, identified by 16S rDNA PCR and sequencing. Identified isolates tagged; U01, U02, U03, U04, S08, U10 and U11 were from urine specimens, S05, S06, S07 and S12 from stool, while B09 was from blood. The isolates were screened for MDR pattern according to Kirby-Bauer disc diffusi

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Said, Layla Abdulhamed, Sawsan Saeed Hasan, and Saad L. Hamed. "Identification of Clinical Pseudomonas spp. by VITEK 2 Compact System and Species-specific Polymerase Chain Reaction Assay for Identification of Pseudomonas aeruginosa." International Journal of Drug Delivery Technology 10, no. 03 (2020): 383–88. http://dx.doi.org/10.25258/ijddt.10.3.14.

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The objective of this study was to isolate, identify, and diagnose Pseudomonas spp. from different clinical sources in Baghdad, Iraq. VITEK 2 compact system identification gram-negative bacteria (ID gNB) cards were used to confirm the identification. Polymerase chain reaction (PCR) technique and sequencing were used for recognition of the 16S rDNA gene, by two pairs of primers, universal primers (930 bp fragments) for recognition of Pseudomonas spp., and Pseudomonas aeruginosa specific species (PASS) primers (956 bp fragments) for differentiation of P. aeruginosa from other species. Amplified

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P, Beulah Rose Rani,, and Sheeba Rajakumari, DV. "Prevalence and Characterization of Disease-Causing Bacteria Isolated from Pigeons (Columba livia) in India." UTTAR PRADESH JOURNAL OF ZOOLOGY 45, no. 18 (2024): 699–704. http://dx.doi.org/10.56557/upjoz/2024/v45i184487.

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Pigeons harbour and spread numerous zoonotic diseases, including bacterial diseases caused by pathogenic bacterial communities. Pigeons are the hosts for various bacteria, including Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, and Streptococcus pyogenes. In the present study, pigeon (Columba livia) from farms and houses were subjected to determine the major causative bacterial pathogens. The samples were collected from the oral cavity, cloaca and ocular regions of pigeons. The distribution of bacteria was tested using the plate count agar method, and

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Arefin, Md Shamsul, Meftahul Jannat Mitu, Shomaia Yasmin Mitu, et al. "Mutational alterations in the QRDR regions associated with fluoroquinolone resistance in Pseudomonas aeruginosa of clinical origin from Savar, Dhaka." PLOS ONE 20, no. 2 (2025): e0302352. https://doi.org/10.1371/journal.pone.0302352.

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Bacterial DNA gyrase and topoisomerase IV are the major targets of quinolone antibiotic, and mutational alterations in quinolone resistance determining regions (QRDR) serve as major mechanism of resistance in most bacterial species, including P. aeruginosa. The present investigation was aimed to study the molecular mechanism of fluoroquinolone resistance among clinical P. aeruginosa isolated from Dhaka, Bangladesh, including alterations in target sites of the antimicrobial action. Laboratory collection of 53 P. aeruginosa were subjected to conventional cultural and biochemical characterization

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Mahgoub, Samir A., Shaza Y. A. Qattan, Salma S. Salem, et al. "Characterization and Biodegradation of Phenol by Pseudomonas aeruginosa and Klebsiella variicola Strains Isolated from Sewage Sludge and Their Effect on Soybean Seeds Germination." Molecules 28, no. 3 (2023): 1203. http://dx.doi.org/10.3390/molecules28031203.

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Phenols are very soluble in water; as a result, they can pollute a massive volume of fresh water, wastewater, groundwater, oceans, and soil, negatively affecting plant germination and animal and human health. For the detoxification and bioremediation of phenol in wastewater, phenol biodegradation using novel bacteria isolated from sewage sludge was investigated. Twenty samples from sewage sludge (SS) were collected, and bacteria in SS contents were cultured in the mineral salt agar (MSA) containing phenol (500 mg/L). Twenty colonies (S1 up to S20) were recovered from all the tested SS samples.

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Joshi, Bhavesh M., Mrugesh M. Patel, Rudra B. Parmar, N. K. Singh, and P. V. Tapre. "Molecular Characterization of Plant Growth Promoting Pseudomonas from Rhizosphere of Euphorbia caducifolia Haines." International Journal of Bio-resource and Stress Management 13, no. 11 (2022): 1240–51. http://dx.doi.org/10.23910/1.2022.3262.

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A study was conducted during kharif 2020 at the Department of Microbiology, Chimanbhai Patel College of Agriculture, S. D. Agricultural University for molecular characterization of plant growth promoting Pseudomonas isolates from rhizosphere of Euphorbia caducifolia. Twenty Pseudomonas isolates (designated as PsEc1 to PsEc20) were axenically isolated from rhizospheric soil sample of Euphorbia caducifolia using Pseudomonas Isolation Agar Base (a selective medium). These isolates were tested gram negative, rod shaped, and oxidase positive. All 20 isolates were positive for utilization of maltose

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Berebon, Dinebari P., Somtochukwu A. Evurani, Anthony A. Attama, et al. "Antimicrobial profiles of probiotic lactic acid bacteria isolated from Nigerian fermented spice condiment Okpeye (<i>Prosopis africana</i>)." Tropical Journal of Pharmaceutical Research 24, no. 2 (2025): 241–48. https://doi.org/10.4314/tjpr.v24i2.12.

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Purpose: To investigate the antimicrobial profile of lactic acid bacteria (LAB), also called friendly bacteria or probiotics, found in Nigerian fermented spice condiment Okpeye (Prosopis africana). Methods: The LAB was isolated by enrichment and serial dilution. Biochemical characterization of isolates was done using standard microbiological protocols and determined using 16S rDNA gene sequencing. The survival of LAB in low pH 2.5 and 0.3 % bile salt media were evaluated. Using the agar well diffusion assay, the cell-free supernatant (CFS) standardized to 0.5 McFarland was evaluated for antiba

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8

Buthaina, H. J., and K. A. Ahmed. "Genetic Features of Pseudomonas Aeruginosa Isolated from Skin Infections." Лабораторная диагностика. Восточная Европа 14, no. 1 (2025): 52–62. https://doi.org/10.34883/pi.2025.14.1.004.

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Introduction. The Gram-negative opportunistic pathogen Pseudomonas aeruginosa, is frequently employed in research on virulence and bacterial resistance. It is a source of nosocomial infections including burns, and surgical site infections. Burn and wound injuries disrupt the skin’s natural defenses, allowing P. aeruginosa to colonize more easily and cause infection due to exposure to matrix proteins and inflammatory factors. Purpose. To perform a genetic analysis of P. aeruginosa isolates in various skin samples (burn and wound), from immunocompromised patients in intensive care units in Nasir

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Rawi, Nurul Najihah, Mujahidah Mohd Ramzi, Nor Izzati Abd Rahman, et al. "Antifouling Potential of Ethyl Acetate Extract of Marine Bacteria Pseudomonas aeruginosa Strain RLimb." Life 13, no. 3 (2023): 802. http://dx.doi.org/10.3390/life13030802.

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Biofouling is defined as the excessive colonization process of epibiotic organisms, ranging from microfoulers to macrofoulers, on any submerged surface in water. Previous research has attempted to explore the antifouling activity of bacterial isolates due to the biofouling problems occurring worldwide. One solution is to inhibit the early stage of fouling using secondary metabolites produced by marine bacteria. This study aims to determine the antifouling activities of the marine microorganism P. aeruginosa and to characterize the bacteria isolated as a potential anti-biofouling agent. The bac

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Saidmurad, Mustafa Azm, Sawsan Sajid Al-Jubori та Mohamed Faraj Edbeib. "Prevalence of Metallo β-Lactamase Genes Among MDR Pseudomonas <i>Aeruginosa</i> Isolated from Different Sources in Baghdad". Al-Mustansiriyah Journal of Science 35, № 3 (2024): 48–57. http://dx.doi.org/10.23851/mjs.v35i3.1470.

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Background: Pseudomonas aeruginosa is a gram-negative apportunistic pathogen that has ability to couse different type of infections and charecterised by their high resistance to commonly used antibiotics via different stratiges. Objective: The objective of this study is to detect the prevalence rate of MβL gene among MDR Pseudomonas aeruginosa. Methods: Forty clinical isolates of Pseudomonas aeruginosa were obtained from different hospitials in Baghdad/Iraq. Their identification was confirmed by using 16s rDNA as a housekeeping gene (reference gene). Specific primers were used to detect 3 type

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Anjum, Hasnain, Md Shamsul Arefin, Nusrat Jahan, et al. "Roles of intrinsic and acquired resistance determinants in multidrug-resistant clinical Pseudomonas aeruginosa in Bangladesh." Bangladesh Journal of Medical Science 22, no. 3 (2023): 489–507. http://dx.doi.org/10.3329/bjms.v22i3.66960.

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Introduction: Pseudomonas aeruginosa is an ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, P. aeruginosa, and Enterobacter spp.) pathogen and one of the leading etiologies in multiple nosocomial infections. Treatment of P. aeruginosa is becoming increasingly difficult due to its ever-increasing antibiotic resistance trends. This study investigated clinical multidrug resistance (MDR) P. aeruginosa (MDR-PA), their intrinsic resistance determinants, including the presence of chromosomal AmpC β-lactamase (Ampicillinase), decreased expression of

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P. Bhosle, Nilesh. "Biodegradation of Carbendazim (CRD) by Microorganisms Isolated from Pesticides Contaminated Soil." Journal of Plant Science Research 38, no. 2 (2023): 861–74. http://dx.doi.org/10.32381/jpsr.2022.38.02.40.

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Excessive use of pesticides has been known to create hazardous to the environment, including affecting soil fertility may also impart toxicity in living organisms. Presently, there have been biological approaches implicated to reduce or eradicate pesticides through physical and chemical methods are inefficient. Microorganisms have a potential to mineralize carbendazim with the help of nutrient minimal mineral salt (MMS) medium and soil. Microorganisms were developed resistant against carbendazim MMS medium and they were identified based on its colony morphological characters and 16s rDNA gene

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Kang, Yu, Yanting Sun, Jinzhong Cui, et al. "Combined microbiome and metabolomics analysis of yupingfeng san fermented by Bacillus coagulans: insights into probiotic and herbal interactions." PeerJ 13 (April 7, 2025): e19206. https://doi.org/10.7717/peerj.19206.

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Background Yupingfeng san is a traditional Chinese medicine formula composed of siler, atractylodes, and astragalus. The herbal medicine fermentation process relies on the role of probiotics. Bacillus coagulans is a probiotic commonly used to ferment food and drugs. It produces a variety of beneficial metabolites during fermentation. However, the study on the interaction between B. coagulans and yupingfeng san is still blank. Methods During solid-state fermentation of yupingfeng san, we used metabolomics technology and 16S rDNA sequencing to analyze the differential metabolites and microbial f

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Ali Sabri Jabbar and Ahmed Majeed Abdzaid. "DNA Sequencing And Phylogenic Tree Analysis Of 16S Rrna And Bla-Oxa-50 Genes Of Carbapenem Resistant Pseudomonas Aeruginosa In Diwanyah City/ Iraq." OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan 2, no. 4 (2024): 95–104. http://dx.doi.org/10.61132/obat.v2i4.516.

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Pseudomonas aeruginosa is a major nosocomial pathogen known for its multidrug resistance, including carbapenems. This study aimed to investigate the prevalence of carbapenem resistance in P. aeruginosa of clinical sources and sequencing of 16S rRNA and blaOXA-50 genes Methods: A total of 53 P. aeruginosa isolates from burn, wound, urinary tract, and ear infections were analyzed. Antibiotic susceptibility testing identified carbapenem-resistant isolates. Polymerase Chain Reaction (PCR) detected 16S rRNA and blaOXA-50 genes, followed by DNA sequencing and phylogenetic analysis of 16S rRNA. Resul

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Drancourt, Michel, Claude Bollet, Antoine Carlioz, Rolland Martelin, Jean-Pierre Gayral, and Didier Raoult. "16S Ribosomal DNA Sequence Analysis of a Large Collection of Environmental and Clinical Unidentifiable Bacterial Isolates." Journal of Clinical Microbiology 38, no. 10 (2000): 3623–30. http://dx.doi.org/10.1128/jcm.38.10.3623-3630.2000.

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Some bacteria are difficult to identify with phenotypic identification schemes commonly used outside reference laboratories. 16S ribosomal DNA (rDNA)-based identification of bacteria potentially offers a useful alternative when phenotypic characterization methods fail. However, as yet, the usefulness of 16S rDNA sequence analysis in the identification of conventionally unidentifiable isolates has not been evaluated with a large collection of isolates. In this study, we evaluated the utility of 16S rDNA sequencing as a means to identify a collection of 177 such isolates obtained from environmen

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Islam, H.S. "Bioremediation of chromium." Islam, H.S., 2016. Bioremediation of chromium. Research Journal of Chemistry and Environment. 20 (January 1, 2016): 34–42. https://doi.org/10.5281/zenodo.8371273.

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In the present study, biosorption of Chromium by microorganisms from Coal mine effluent was investigated. The initial concentration of heavy metal Chromium (K2Cr2O7) in the effluent was 120mg/lit. The bacteria isolated from industrial effluent were identified as Bacillus megaterium and Pseudomonas aeruginosa by biochemical and 16S rDNA sequencing. The effect of pH and temperature on the biosorption capacity was investigated. Under the optimum conditions the highest uptake in pH 7 at 300C was 96% and 90% in case of Bacillus megaterium and 95 and 90% in case of Pseudomonas aeruginosa. At low tem

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Lawton, Samantha J., Allison M. Weis, Barbara A. Byrne, et al. "Comparative analysis of Campylobacter isolates from wild birds and chickens using MALDI-TOF MS, biochemical testing, and DNA sequencing." Journal of Veterinary Diagnostic Investigation 30, no. 3 (2018): 354–61. http://dx.doi.org/10.1177/1040638718762562.

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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was compared to conventional biochemical testing methods and nucleic acid analyses (16S rDNA sequencing, hippurate hydrolysis gene testing, whole genome sequencing [WGS]) for species identification of Campylobacter isolates obtained from chickens ( Gallus gallus domesticus, n = 8), American crows ( Corvus brachyrhynchos, n = 17), a mallard duck ( Anas platyrhynchos, n = 1), and a western scrub-jay ( Aphelocoma californica, n = 1). The test results for all 27 isolates were in 100% agreement between MALDI

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Hussein, Zainab T., Suaad S. Mukhlif, Mohammed Taha, and Suha Abduhakeem Ali. "Identification and Characterization of Pseudomonas aeruginosa 16S rRNA Gene Isolated from Contaminated Soil With Oil Residues." IOP Conference Series: Earth and Environmental Science 1215, no. 1 (2023): 012001. http://dx.doi.org/10.1088/1755-1315/1215/1/012001.

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Abstract Pseudomonas aeruginosa was isolated from fifty soil samples were collected from different sites of contaminated soil with oil residues in Ministry of science& technology. Morphological, biochemical tests and 16S rRNA genes sequencing was performed for bacterial isolates identification. Thirty (30) isolates of P. aeruginosa bacteria were confirmed according to morphological, biochemical tests. For molecular analysis, all isolates that tested positive for P. aeruginosa underwent amplification of the 16S rRNA gene using previously described primers that amplified a particular DNA fra

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Girdhar, Anurag, Jagdish Chinnappa, Feroze Ganaie, Vandana Govindan, Kadahalli Ravikumar, and Geetha Nagaraj. "Bacterial Profile of Middle Ear Fluid with Recurrent Acute Otitis Media Infection Using Culture Independent 16S rDNA Gene Sequencing." Journal of Pediatric Infectious Diseases 14, no. 03 (2018): 108–15. http://dx.doi.org/10.1055/s-0038-1675786.

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Background Recurrent otitis media is one of the common infections of childhood. The causative bacterial pathogen is one of the major risk factors of recurrent infection. With limited availability of Indian data, we performed this study to identify the bacterial pathogens. Materials and Methods Otitis media cases were diagnosed based on clinical criteria. Thirty-six middle ear fluid (MEF) samples were collected by tympanocentesis and cultured for pathogens. Seventy-eight per cent of the cases had three previous episodes of otitis media in the past 6 months; the remaining 22% had four episodes i

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Agbo, Ejiofor C., Obiora R. Ejinaka, Obiorah E. Okechukwu, Obeta M. Uchejeso, Jwanse I. Rinpan, and Ifeoma E. Lote-Nwaru. "Molecular Identification of Biofilm-forming Pseudomonas aeruginosa Isolates from Noncritical Surfaces of a Tertiary Healthcare Center in Abia State, Southeast Nigeria." Journal of Biochemistry, Microbiology and Biotechnology 10, no. 1 (2022): 25–30. http://dx.doi.org/10.54987/jobimb.v10i1.659.

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The presence of biofilm-forming Pseudomonas aeruginosa on noncritical surfaces in a hospital has been attributed to healthcare-associated infections (HAIs) leading to multidrug-resistant (MDR) infections. This study is aimed at identifying the molecular characteristics of Biofilm forming Pseudomonas aeruginosa found on non-critical surfaces in a hospital environment. Samples were collected using the swab technique from different noncritical surfaces surrounding hospitalized patients in different wards namely sphygmomanometers, thermometers, bed rails etc. Bacterial analysis was performed by us

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Harrigan, James, Ebbing Lautenbach, Emily Reesey, et al. "Impact of Diagnosed and Undiagnosed Respiratory Pseudomonas on VAP and VAE During Long-Term Acute Care." Infection Control & Hospital Epidemiology 41, S1 (2020): s258—s259. http://dx.doi.org/10.1017/ice.2020.823.

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Background: Clinically diagnosed ventilator-associated pneumonia (VAP) is common in the long-term acute-care hospital (LTACH) setting and may contribute to adverse ventilator-associated events (VAEs). Pseudomonas aeruginosa is a common causative organism of VAP. We evaluated the impact of respiratory P. aeruginosa colonization and bacterial community dominance, both diagnosed and undiagnosed, on subsequent P. aeruginosa VAP and VAE events during long-term acute care. Methods: We enrolled 83 patients on LTACH admission for ventilator weaning, performed longitudinal sampling of endotracheal aspi

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Subrahmanyam, G., R. Das, R. Debnath, M. Chutia, K. M. Ponnuvel, and K. Sathyanarayana. "Characterization of bacterial pathogens in Muga silkworm, Antheraea assamensis Helfer (Lepidoptera: Saturniidae)." Journal of Environmental Biology 44, no. 3(SI) (2023): 479–84. http://dx.doi.org/10.22438/jeb/44/3(si)/jeb-07.

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Aim: To identify and characterize the bacterial pathogens associated with bacteriosis in muga silkworm, A. assamensis. Methodology: The mid gut of diseased silkworm, A. assamensis was dissected and bacterial pathogens were cultured. Bacterial pathogens were identified by 16S rRNA gene sequencing. Insect bioassay studies were conducted to understand the pathogenicity of bacterial isolates. Results: Bacterial pathogens were identified as Pseudomonas aeruginosa and Bacillus mycoids. Under laboratory conditions, approximately 70% mortality of A. assamensis larvae was due to P. aeruginosa, while 50

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Alghreri, Ansam Adnan Ahmed, Kawkab Abdalla Alsaadi, and Hussein O. M. Al-Dahmoshi. "Prevalence of blaIMP-1 among P. aeruginosa isolated from patients with burn infections." Journal of Applied and Natural Science 14, no. 3 (2022): 1062–66. http://dx.doi.org/10.31018/jans.v14i3.3777.

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Carbapenems are often used as antibiotics for treating infections by multidrug-resistant Gram-negative bacteria. Pseudomonas aeruginosa is considered a major cause of health care issues associated with higher mortality and morbidity rates worldwide. This study aimed to detect the prevalence of carbapenemase gene blaIMP-1 that is produced by carbapenem-resistant P. aeruginosa isolated from burn infection patients. A total of 137 burn swabs were collected from burn patients in different hospitals in Babylon province from December 2021 to April 2022. All of the swabs were streaked on selective me

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Gu, F., Y. Li, C. Zhou, et al. "Bacterial 16S rRNA/rDNA Profiling in the Liquid Phase of Human Saliva." Open Dentistry Journal 3, no. 1 (2009): 80–84. http://dx.doi.org/10.2174/1874210600903010080.

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Human saliva can be separated by centrifugation into cell pellet and cell-free supernatant, which are called cellular phase and liquid phase in this study. While it is well documented that the cellular phase of saliva contains hundreds of oral bacteria species, little is known whether the liquid phase of saliva contains any information related to oral microbiota. In this study, we analyzed the bacterial nucleic acid contents of the liquid phase of saliva. Using primers universal to most eubacterial 16S rDNA, we detected large amounts of bacterial 16S rRNA and rDNA in the cell-free phase of sal

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Jamaluddin, Indra P., Susan H. Musa, Stalis N. Ethica, et al. "Detection of Pseudomonas aeruginosa pus wound isolate using a polymerase chain reaction targeting 16S rRNA and gyrB genes: A case from Indonesia." Narra J 4, no. 2 (2024): e774. http://dx.doi.org/10.52225/narra.v4i2.774.

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Infectious wounds on the skin surface are easily colonized by bacteria from pyogenic group that manifest as inflammation, such as Pseudomonas aeruginosa. P. aeruginosa is a Gram-negative bacterium and an opportunistic pathogen known for causing invasive state in critically ill and immunocompromised patients. The aim of this study was to detect the 16S rRNA and gyrB genes in P. aeruginosa using polymerase chain reaction (PCR) method. The sample in this study was pus isolate from a 5-year-old boy with leg wounds. The bacteria were isolated on brain heart infusion broth (BHIB) media and identifie

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Khazaal, Saba Saadoon, Yasir B. Fadhil, Mustafa Hasan Talha, and Shaymaa Khudhr Al-Alak. "Detection of some virulence genes among clinical Pseudomonas aeruginosa isolates in Baghdad." Reviews and Research in Medical Microbiology 35, no. 2 (2023): 107–11. http://dx.doi.org/10.1097/mrm.0000000000000348.

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Purpose: The objectives of the current study are to detect some important virulence genes in Pseudomonas aeruginosa, which isolated from clinical samples in Baghdad's different hospitals. Methods: A total of 40 clinical P. aeruginosa isolates were collected from different hospitals in Baghdad city. The isolates were identified using morphological tests and confirmed by conventional PCR by using specific primer for 16S rDNA gene. To extract DNA from the isolates, a genomic DNA kit was used molecular detection of two virulence genes: ompA and papC, which were amplified by PCR. Results: All 40 is

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Girsova, Natalya, Denis Erokhin, Dmitriy Vorobyov, Damir Bogoutdinov, and Tatyana Kastalyeva. "Identification of 16SrXII-A and 16SrXII-H Phytoplasma Subgroup in Russia, Using 16S rDNA NGS Analysis." Ratarstvo i povrtarstvo, no. 00 (2025): 8. https://doi.org/10.5937/ratpov61-51638.

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The RFLP analysis of the 16S rDNA revealed two distinct phytoplasma groups, 16SrXII-A and 16SrXII-H, suggesting the presence of diverse phytoplasma strains within the analyzed isolates from the Russian State Collection of Pathogenic Microorganisms. Partial 16S rDNA sequences of four phytoplasma, 'Candidatus Phytoplasma', isolates belonging to the 16SrXII group from the three plant species: common hop (Humulus lupulus L.), Indian datura (Datura metel L.) and grapevine (Vitis vinifera L.) are presented. DNA of gene coding 16S rRNA was amplified, using nested PCR with primers P1/16Sr-SR and R16F2

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Bayode, Michael Tosin, Adewale Oluwasogo Olalemi, and Babayemi Olawale Oladejo. "Multiple antibiotic resistant index and detection of qnrS and qnrB genes in bacterial consortium of urine samples from clinical settings." European Journal of Biological Research 11, no. 1 (2021): 45–56. https://doi.org/10.5281/zenodo.4304311.

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The multiple antibiotic resistant (MAR) index and detection of resistant genes in the bacterial consortium of urine samples collected from University of Medical Sciences Teaching Hospital, Akure (UNIMEDTH) was evaluated with all microbiological and biotechnological techniques employed utilizing specified standards in this study. <em>Escherichia coli </em>had the highest bacterial count (311.50 ± 0.707 CFU/ml) while <em>Staphylococcus saprophyticus </em>had the least (13.00 ± 2.828 CFU/ml). <em>Enterococcus faecalis</em>, and <em>Pseudomonas</em> <em>aeruginosa</em> isolate showed

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Costa, Jackeliny dos Santos, Thalita Priscila Peres Seabra Da Cruz, Larissa Viera Da Costa, et al. "Ocorrência de genes de resistência metalo-beta-lactamases em isolados de Pseudomonas aeruginosa MDR de otites de cão, Brasil." OBSERVATÓRIO DE LA ECONOMÍA LATINOAMERICANA 21, no. 8 (2023): 8722–34. http://dx.doi.org/10.55905/oelv21n8-049.

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A Pseudomonas aeruginosa é uma bactéria oportunista, considerada um dos principais agentes de infecção nosocomial em hospitais associada múltipla resistência a antimicrobianos. Em animais, principalmente cães, a otite é uma enfermidade de grande incidência associado a P. aeruginosa. A ocorrência de isolados multirresistentes a metalo beta lactamase com presença de genes blaIMP-1, blaVIM-2, blaSPM-1 e blaNDM-1 tem sido bem descrita em isolados humanos entretanto a ocorrência em animais são escassos. O objetivo deste trabalho foi a detecção dos genes de metalo-beta-lactamses em isolados de P. ae

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Gosal, S. K., G. S. Saroa, Y. Vikal, S. S. Cameotra, Neemisha Pathania, and A. Bhanot. "Isolation and molecular characterisation of diazotrophic growth-promoting bacteria from wheat rhizospheric soils of Punjab." Soil Research 49, no. 8 (2011): 725. http://dx.doi.org/10.1071/sr11136.

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Diazotrophs are nitrogen-fixing bacteria which possess the nifH gene that codes for the nitrogenase enzyme involved in reduction of atmospheric dinitrogen to ammonia. Seventy-two diazotrophic bacteria were isolated using eight nitrogen-free media from wheat rhizospheric soil. The diazotrophic population was found to be negatively related to soil nitrogen, whereas a positive correlation was observed with organic carbon and electrical conductivity of soil. The isolates were initially identified on the basis of cultural, morphological, and biochemical characterisation. Various diazotrophic isolat

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de Souza, Paula Araujo, Milena Cristina Silva dos Santos, Rebeca Vitória da Silva Lage de Miranda, et al. "Evaluation of Antimicrobial Resistance Patterns of Pseudomonas aeruginosa Strains Isolated among COVID-19 Patients in Brazil Typed by Fourier-Transform Infrared Spectroscopy." Life 14, no. 9 (2024): 1079. http://dx.doi.org/10.3390/life14091079.

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This study aimed to characterize Pseudomonas aeruginosa strains isolated from hospitalized patients during the COVID-19 pandemic. This was achieved using phenotypic and molecular techniques, including their antimicrobial resistance profile and biofilm formation. Eighteen strains were isolated from a hospital in Rio de Janeiro, Brazil, and identified by VITEK®2, MALDI-TOF/MS (VITEK MS® and MALDI Biotyper®), and 16S rRNA sequencing. Fourier-transform infrared (FTIR) spectroscopy, antimicrobial susceptibility testing, and biofilm formation and disinfectant tolerance tests were applied to evaluate

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Uddin, Fakhur, Muhammad Sohail, Qurban Hussain Shaikh, Mir Tahir Hussain, Kerry Roulston, and Timothy Daniel McHugh. "Verona integron encoded metallo Beta lactamase (VIM) and Vietnam extended spectrum Beta lactamase (VEB) producing Pseudomonas balearica from a clinical specimen." Journal of the Pakistan Medical Association 72, no. 4 (2022): 761–63. http://dx.doi.org/10.47391/jpma.3890.

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Pseudomonas balearica, a saprophyte found in marshy and marine habitats, is not routinely differentiated from P. aeruginosa and P. stutzeri using automated systems and hence has not been reported from clinical samples. This study describes the identification of P. balearica using MALDI-TOF-MS and 16S rDNA sequence from a patient admitted to an intensive care unit (I.C.U.). The isolate was found to be Verona integron-mediated Metallo-Beta-lactamase (V.I.M.), and Vietnam extended-spectrum Beta-lactamase (V.E.B.) producer and resistant to Ceftriaxone, Imipenem, and Tobramycin. P. balearica can be

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Homem de Mello de Souza, Helena Aguilar Peres, Libera Maria Dalla-Costa, Fernando José Vicenzi, et al. "MALDI-TOF: A useful tool for laboratory identification of uncommon glucose non-fermenting Gram-negative bacteria associated with cystic fibrosis." Journal of Medical Microbiology 63, no. 9 (2014): 1148–53. http://dx.doi.org/10.1099/jmm.0.076869-0.

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The predisposition of patients with cystic fibrosis (CF) for recurrent pulmonary infections can result in poor prognosis of the disease. Although the clinical significance in CF of micro-organisms, such as Staphylococcus aureus, Haemophilus influenzae and Pseudomonas aeruginosa, is well established, the implication of uncommon glucose non-fermenting Gram-negative bacilli (UGNF-GNB) in respiratory samples from CF patients is still unclear. Because of limitations of traditional methods used in most clinical laboratories, the accurate identification of these microbes is a challenge. Matrix-assist

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Aibuedefe, Eremwanarue Osagie, Nwawuba Stanley Udogadi, and Shittu Olalekan Hakeem. "Characterisation of the Prevailing Multidrug Pseudomonas aeruginosa Strains from Surgical Wound Using 16S rRNA Sequencing Technique." Malaysian Journal of Medical Sciences 28, no. 4 (2021): 37–49. http://dx.doi.org/10.21315/mjms2021.28.4.5.

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Background: Pseudomonas aeruginosa (P. aeruginosa) is prevalent in hospital-acquired surgical wound infections. It exhibits both innate and acquired resistance to a broad range of antimicrobials and remains a principal problem in clinical practice. Methods: In total, 284 sterile surgical wound swabs (142 each) were collected from two government hospitals: Central Hospital Benin (CHB) and University of Benin Teaching Hospital (UBTH) in Benin City, Nigeria. Pseudomonas spp. isolated from both hospitals were screened with eight different antibiotics by way of disk diffusion method. Polymerase cha

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Li, Juan, Yanchen Wen, and Xiangdong Yang. "Understanding the Responses of Soil Bacterial Communities to Long-Term Fertilization Regimes Using DNA and RNA Sequencing." Agronomy 11, no. 12 (2021): 2425. http://dx.doi.org/10.3390/agronomy11122425.

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Studies of soil DNA-based and RNA-based bacterial communities under contrasting long-term fertilization regimes can provide valuable insights into how agricultural management affects soil microbial structure and functional diversity. In this study, soil bacterial communities subjected to six fertility treatments in an alkaline soil over 27 years were investigated by 454 pyrosequencing based on 16S rDNA and 16S rRNA. Long-term fertilization showed significant influences on the diversity of the soil DNA-based bacteria, as well as on their RNA-based members. The top five phyla (Proteobacteria, Ac

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Li, Xu-Ming, Qing Lv, Ya-Jun Chen, Lu-Biao Yan, and Xin Xiong. "Association between childhood obesity and gut microbiota: 16S rRNA gene sequencing-based cohort study." World Journal of Gastroenterology 30, no. 16 (2024): 2249–57. http://dx.doi.org/10.3748/wjg.v30.i16.2249.

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BACKGROUND This study aimed to identify characteristic gut genera in obese and normal-weight children (8-12 years old) using 16S rDNA sequencing. The research aimed to provide insights for mechanistic studies and prevention strategies for childhood obesity. Thirty normal-weight and thirty age- and sex-matched obese children were included. Questionnaires and body measurements were collected, and fecal samples underwent 16S rDNA sequencing. Significant differences in body mass index (BMI) and body-fat percentage were observed between the groups. Analysis of gut microbiota diversity revealed lowe

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Wang, Zongwu, Wantao Tian, Siyuan Sun, Xing Chen, and Haifeng Wang. "Genomic and Proteomic Analysis of Pseudomonas aeruginosa Isolated from Industrial Wastewater to Assess Its Resistance to Antibiotics." Separations 10, no. 11 (2023): 549. http://dx.doi.org/10.3390/separations10110549.

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Industrial wastewater usually contains a large amount of organic and inorganic pollutants, and many microorganisms. However, the types of microorganism present in industrial wastewater are still unclear. The aim of this study was to analyze the physicochemical properties and drug resistance of Pseudomonas aeruginosa isolated from industrial wastewater containing high concentrations of sulfate compounds. Pseudomonas aeruginosa was isolated from industrial wastewater from industrial produce with high concentrations of sulfate and phosphate, and mass spectrometry identification, gene identificati

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Bathini, Nagaraju, Vishnuvardhan Reddy Sultanpuram, and Thirumala Mothe. "Isolation, identification and mutants generation of isolate 04 for its improved bioconversion of ferulic acid to vanillin." Journal of Applied Biological Sciences 19, no. 1 (2025): 23–29. https://doi.org/10.71336/jabs.1381.

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Vanillin is widely used as a food flavouring, scent ingredient in cosmetics and perfumes, as an intermediate in agrochemicals & medicines and also as a nutraceutical. Serial dilution of sediment sample yielded 32 strains, among which three isolates A2, A7, and 04 reported here were characterized at molecular level by 16S rDNA sequencing. They were identified and similar to Pseudomonas aeruginosa (94.97%), Acinetobacter baumannii (99.93%) and Lysinibacillus macroides (98.67%) respectively based on its 16S rDNA sequences. Among these three strains, isolate 04 was with high ferulic acid (FA)

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Fosch, Sonia E., Cecilia A. Ficoseco, Antonella Marchesi, Silvina Cocucci, Maria E. F. Nader-Macias, and Beatriz E. Perazzi. "Contraception: Influence on Vaginal Microbiota and Identification of Vaginal Lactobacilli Using MALDI-TOF MS and 16S rDNA Sequencing." Open Microbiology Journal 12, no. 1 (2018): 218–29. http://dx.doi.org/10.2174/1874285801812010218.

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Background:The vaginal microbiome is influenced by a wide variety of factors, including contraceptive methods.Aim:To evaluate the effect of contraceptive methods on vaginal microbiota and to compare MALDI-TOF MS and 16S rDNA sequencing for lactobacilli identification.Patients and Methods:One hundred and one (101) women consulting for birth control were included in a prospective study. Their vaginal content was sampled and analyzed once before they started using the contraceptive method of their choice, and twice after the initiation of contraception, at three months (94/101 women attended) and

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de Vries, Jacob, Faizan Saleem, Enze Li, et al. "Comparative Analysis of Metagenomic (Amplicon and Shotgun) DNA Sequencing to Characterize Microbial Communities in Household On-Site Wastewater Treatment Systems." Water 15, no. 2 (2023): 271. http://dx.doi.org/10.3390/w15020271.

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The performance of on-site wastewater treatment systems (OWTSs) can be improved by altering digester design and by manipulating environmental variables that affect microbial community composition. Community composition can potentially be assessed using high-throughput DNA sequencing, but the two most common methods of community DNA sequencing (16S and shotgun sequencing) generally yield different taxonomic identification profiles and can perform differently according to the sampled environment. To evaluate the use of these two approaches in monitoring OWTS operation, we conducted a comparative

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Noskova, Svetlana, Svetlana Ivanova, Alexander Prosekov, et al. "Study of the Antimicrobial Potential of Bacteria found in Natural Resources." Journal of Pure and Applied Microbiology 15, no. 2 (2021): 759–71. http://dx.doi.org/10.22207/jpam.15.2.28.

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Bacteriocins are of great interest as potential antimicrobial agents against various types of bacteria, fungi, and viruses. Isolates of microorganisms derived from natural sources were used in the current study, including lactic acid bacteria and other antagonistic microorganisms. The species of the microorganisms were determined using 16S rDNA and ITS nrDNA analyses. E. coli, S. enterica, S. aureus, P. aeruginosa, B. mycoides, A. faecalis, P. vulgaris, S. flexneri , L. monocytogenes, C. albicans, A. flavus, and P. citrinum were used as pathogenic and opportunistic strains. It was found that 1

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Scortichini, Marco, Maria Pia Rossi, Stefania Loreti, et al. "Pseudomonas syringae pv. coryli, the Causal Agent of Bacterial Twig Dieback of Corylus avellana." Phytopathology® 95, no. 11 (2005): 1316–24. http://dx.doi.org/10.1094/phyto-95-1316.

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Thirty-eight bacterial strains isolated from hazelnut (Corylus avellana) cv. Tonda Gentile delle Langhe showing a twig dieback in Piedmont and Sardinia, Italy, were studied by a polyphasic approach. All strains were assessed by fatty acids analysis and repetitive sequence-based polymerase chain reaction (PCR) fingerprinting using BOX and ERIC primer sets. Representative strains also were assessed by sequencing the 16S rDNA and hrpL genes, determining the presence of the syrB gene, testing their biochemical and nutritional characteristics, and determining their pathogenicity to hazelnut and oth

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AL-Zwaid, Afrah Jawad Abd, and Hussein Oleiwi Muttaleb Al-Dahmoshimoshi. "Molecular investigation of Pseudomonas aeruginosa mexAB-oprM efflux pump genes from clinical samples and their correlation with antibiotic resistance." Journal of Applied and Natural Science 14, no. 1 (2022): 140–47. http://dx.doi.org/10.31018/jans.v14i1.3240.

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Pseudomonas aeruginosa, one of the majority of common opportunistic infections, has become a public health concern, exhibiting intrinsic and acquired resistance to a wide range of antimicrobials. The present work aimed to study the correlation between the P. aeruginosa efflux pump mexAB-oprM genes and antibiotic resistance to different types of antibiotics. All 79 isolates were screened by Pseudomonas chromogenic agar, which was used as a selective medium for the isolation of P. aeruginosa. After incubation at 37°C for 24 hr, the results were confirmed by PCR using specific primer pairs for th

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Ram, Nanda, Vishal Sharma, Indu Singh Sankhla, and Kailash Agrawal. "Association of Pseudomonas syringae pv. apii with the Seed of Ajwain (Trachyspermum ammi L.)." Journal of Plant Science Research 38, no. 2 (2023): 883–92. http://dx.doi.org/10.32381/jpsr.2022.38.02.42.

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Present study aimed to isolate, identify and characterize the bacterial pathogens associated with ajwain seeds using biochemical and molecular methods. One hundred thirty-nine seed samples of ajwain were collected from various districts of Rajasthan. Twenty-four bacterial isolates were isolated and purified from collected seed samples. All strains were characterised for their biochemical activity. All isolates were tested for genetic diversity by using amplified rDNA restriction analysis (ARDRA). Selective strains were subjected for 16S rDNA sequencing and molecular phylogeny study. Most strai

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Li, Binbin, Shuji Liu, Xiaoting Chen, et al. "Dynamic Changes in the Microbial Composition and Spoilage Characteristics of Refrigerated Large Yellow Croaker (Larimichthys crocea) during Storage." Foods 12, no. 21 (2023): 3994. http://dx.doi.org/10.3390/foods12213994.

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The quality changes, dynamic changes in microbial composition, and diversity changes in large yellow croaker (Larimichthys crocea) during 4 °C refrigeration were studied using 16S rDNA high-throughput sequencing technology, and the total viable count (TVC), total volatile basic nitrogen (TVB-N), and thiobarbituric acid-reactive substances (TBARS) were determined. The results revealed a consistent increase in TVC, TVB-N, and TBARS levels over time. On the 9th day, TVC reached 7.43 lg/(CFU/g), while on the 15th day, TVB-N exceeded the upper limit for acceptable quality, reaching 42.56 mg/100 g.

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Marques, Eder, Rafaela CF Borges, and Carlos H. Uesugi. "Identification and pathogenicity of Pseudomonas cichorii associated with a bacterial blight of gerbera in the Federal District." Horticultura Brasileira 34, no. 2 (2016): 244–48. http://dx.doi.org/10.1590/s0102-053620160000200015.

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ABSTRACT: In 2013, leaf samples of gerbera plants showing symptoms of bacterial blight were collected in cut-flower high tunnels, in the settlement of núcleo rural alexandre gusmão, located in brazlândia, distrito federal, brazil. seven isolates obtained were subjected to phenotypic and molecular characterization, including pathogenicity tests, lopat, and partial sequencing of the 16s rdna gene. all isolates were gram-negative, aerobic, oxidase-positive, produced fluorescent pigment, induced hypersensitivity in tobacco leaves, used sorbitol and glutamate and were pathogenic to 24 different pla

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Liu, Zerui, Yu Guo, Chuanxin Qin, Xiaohui Mu, and Jia Zhang. "High-Throughput Sequencing Analysis Revealed a Preference for Animal-Based Food in Purple Sea Urchins." Biology 13, no. 8 (2024): 623. http://dx.doi.org/10.3390/biology13080623.

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Sea urchins play an important role in marine ecosystems. Owing to limitations in previous research methods, there has been insufficient understanding of the food sources and ecological functional value of purple sea urchins, leading to considerable controversy regarding their functional positioning. We focused on Daya Bay as the research area, utilizing stable isotope technology and high-throughput sequencing of 16S rDNA and 18S rDNA to analyze sea urchins and their potential food sources in stone and algae areas. The results showed that the δ13C range of purple sea urchins in the stone area i

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Ajao, Abdullahi, and Oluwabukola Kudirat Jimoh-Hamza. "EXPLORING THE EFFECTIVENESS OF Pseudomonas aeruginosa ISOLATES FOR BIOREMEDIATION OF CRUDE OIL-CONTAMINATED SOILS USING SOYBEAN HULL AS A BIOSTIMULANT: A FOCUS ON ETPH AND PAHs." FUDMA JOURNAL OF SCIENCES 8, no. 4 (2024): 296–302. http://dx.doi.org/10.33003/fjs-2024-0804-2575.

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The Speciated EPA-16 Polyaromatic Hydrocarbons (PAHs) in crude oil pose significant environmental and health risks due to their harmful properties. This study focused on indigenous bacteria capable of degrading Extractable Total Petroleum Hydrocarbons (ETPHs) and PAHs through a Nutrient-Amended Bioaugmentation Strategy (N-ABS). Crude oil-degrading bacteria were isolated from contaminated soil in Alakiri Community, Rivers State, Nigeria, leading to the identification of four Pseudomonas aeruginosa isolates (KUD1-4) via 16S rRNA gene sequencing. Bioremediation treatments, using both bacterial is

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Hasan, R., M. N. H. Rony, M. S. A. Sarker, M. Z. Tareq, and R. Ahmed. "Isolation, identification and characterization of bacteria from Lawachara National Park, Moulovibazar, Bangladesh." Journal of Bioscience and Agriculture Research 26, no. 02 (2020): 2211–16. http://dx.doi.org/10.18801/jbar.260220.270.

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Bacteria are essential elements of natural environments. As bacteria are the key critical components of food webs and nutrient cycles, they contribute to ecosystem functioning via mutualists and pathogens for larger species. The present study has provided substantial grounds to confirm that microbial communities present in natural environments are much more diverse. Here, we tend to study a singular environment Lawachara National Park. Total 125 bacterial strains were isolated using serial dilution method. Thirteen unique colonies were selected, cultured and characterized by gram staining and

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Usta, Mustafa, and Abdullah Güller. "Detection and Molecular Characterization of ‘Candidatus Phytoplasma Trifolii’, a Member of the Clover Proliferation Grup, Infecting Tomato Plants from Iğdır Province in Turkey." Turkish Journal of Agriculture - Food Science and Technology 8, no. 12 (2020): 2533–40. http://dx.doi.org/10.24925/turjaf.v8i12.2533-2540.3592.

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The tomato plant exhibiting leaf rolling, witches’ broom, distorted and elongated ﬂower’s sepals in Iğdır province, Turkey, was observed. Total DNA extraction was performed from the symtomatic fresh tomato sample. All DNAs were subjected to Direct and Nested polymerase chain reaction (PCR) with universal primer sets that amplified the 16S rRNA of phytoplasmas. PCR products were purified from agarose gel and cloned into the pGEM T-Easy cloning vector. Recombinant plasmids were introduced into the prokaryotic cloning bacteria by electroporation. Plasmid isolation was performed by selecting one o

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