Relevant bibliographies by topics / Agar well diffusion assay

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers
  5. Reports

Academic literature on the topic 'Agar well diffusion assay'

Author: Grafiati
Published: 3 June 2025
Last updated: 31 July 2025

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Journal articles on the topic "Agar well diffusion assay"

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Esra, Demir, and Başbülbül Gamze. "Screening of Bacteriocin Production in Lactic Acid Bacteria Isolated From Fermented Dairy Products." Biotechnology Journal International 18, no. 2 (2017): 1–9. https://doi.org/10.9734/BJI/2017/33504.

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<strong>Aims: </strong>In this study we aimed to screen LAB strains from different fermented dairy products by phenotypic and genotypic methods. <strong>Methodology:</strong> 138 LAB isolates belonging to <em>Enterococcus</em> (13,76%), <em>Lactococcus</em> (18,84%) and <em>Lactobacillus</em> (67,39%) genera from fermented dairy products were used to search for their bacteriocin production and bacteriocin encoding genes were searched. LAB isolates were screened by spot-on lawn assay method against indicator microorganisms. <em>Geobacillus stearothermophilus</em> DSMZ 22, <em>Escherichia coli</
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Chigozie, Celestina Ezeagha, Ogechukwu Ogbuebuna Jacinta, Nwanyioma Anozie Amara, Chuks Oranu Emmanuel, and Henrietta Nedum Chinenye. "Determination of the Phytochemical and Antimicrobial properties of Nauclea latifolia Root Smith. (Rubiaceae)." GSC Biological and Pharmaceutical Sciences 23, no. 2 (2023): 182–88. https://doi.org/10.5281/zenodo.8041513.

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<em>Nauclea latifolia&nbsp;</em>is a valuable medicinal plant for treating different ailments. Infectious diseases constitute a world health concern. This work describes the phytochemical analysis and antimicrobial properties of the root of&nbsp;<em>N. latifolia</em>. Collected root samples were extracted via cold maceration with methanol. The methanol crude extract was fractionated into n-hexane, ethyl acetate, butanol and aqueous fractions. Phytochemical analyses and antimicrobial activity of the methanol extract/ fractions were assessed using agar well diffusion method. Four different fract
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Loureno, Felipe Rebello, Telma Mary Kaneko, and Terezinha De Jesus Andreoli Pinto. "Validation of Erythromycin Microbiological Assay Using an Alternative Experimental Design." Journal of AOAC INTERNATIONAL 90, no. 4 (2007): 1107–10. http://dx.doi.org/10.1093/jaoac/90.4.1107.

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Abstract The agar diffusion method, widely used in antibiotic dosage, relates the diameter of the inhibition zone to the dose of the substance assayed. An experimental plan is proposed that may provide better results and an indication of the assay validity. The symmetric or balanced assays (2 2) as well as those with interpolation in standard curve (5 1) are the main designs used in the dosage of antibiotics. This study proposes an alternative experimental design for erythromycin microbiological assay with the evaluation of the validation parameters of the method referring to linearity, precis
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Zainab, Kashmala, and Hira Batool. "A Simplistic Screening Assay of Antimicrobial Compounds and Enzymatic Activity from Local Soil Microbes." RADS Journal of Biological Research & Applied Sciences 9, no. 1 (2018): 24–29. http://dx.doi.org/10.37962/jbas.v9i1.118.

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Antibiotics production is the most emerging field worldwide with a constant need for the new ones to fight the microbial resistance. In this context, the research was pursued to isolate, characterize and screen for promising antibiotic-producing microbes from local soil. The soil bacterial isolates (S1, S2, S3,S4, and S5) and fungal isolates (F1, F2, F4, F6, and F7) were selected and screened for antimicrobial activity against the Test bacteria by agar well diffusion and disc diffusion methods. Assays for Extracellular enzymes including Protease, Lipase, Lecithinase, Cellulase, and Amylase fol
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Şentürk, Esra, Sema Yakıt, and Pınar Şanlıbaba. "Antilisterial Activity by Enterococcus Species Isolated from Traditional Cheeses." Turkish Journal of Agriculture - Food Science and Technology 8, no. 7 (2020): 1593–97. http://dx.doi.org/10.24925/turjaf.v8i7.1593-1597.3503.

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In this study, it was aimed to detect the antimicrobial activity of 312 Enterococcus species against Listeria monocytogenes. Antimicrobial activity was detected by agar spot and well diffusion assay. A total of 201 enterococcal strains inhibited the growth of L. monocytogenes strains based on the agar spot test. Only 44 strains showed antimicrobial activity against L. monocytogenes strains using agar well diffusion assay. Of the 44 enterococcal strains screened, 6 E. faecium (2.99%) strains had a high antimicrobial effect against indicator L. monocytogenes strains. The antilisterial activity o
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Saranya, Shankar, and Sathiavelu Mythili. "Endophytic fungus - Albifimbria verrucaria isolated from Centella asiatica and its antioxidant and antibacterial activities." Research Journal of Biotechnology 20, no. 2 (2024): 92–98. https://doi.org/10.25303/202rjbt092098.

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The present research focuses on the isolation of endophytic fungi from the roots of Centella asiatica and analyzes the antagonistic efficiency of endophytic fungus against bacterial fish pathogens. Agar plug diffusion assay, phytochemical screening, antioxidant activity, agar well diffusion assay and minimum inhibitory and bactericidal concentration were done for the fungal endophytes. Eight endophytic fungi in total were isolated from C.asiatica roots. Among that, CAR1 exhibits maximum antibacterial action in the preliminary screening and it is subjected to an extraction procedure using diffe
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King, Thea, Gary Dykes, and Ruth Kristianti. "Comparative Evaluation of Methods Commonly Used to Determine Antimicrobial Susceptibility to Plant Extracts and Phenolic Compounds." Journal of AOAC INTERNATIONAL 91, no. 6 (2008): 1423–29. http://dx.doi.org/10.1093/jaoac/91.6.1423.

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Abstract A comparison was made to evaluate the ability of the most commonly used qualitative agar diffusion methods and a quantitative broth dilution assay to determine the antimicrobial activity of a plant extract and a variety of phenolic compounds. A disc and well diffusion technique and a microtiter broth microdilution (MBM) assay were used as antimicrobial susceptibility tests of a plant extract and several phenolic compounds against 7 bacterial species. In both the well and disc diffusion assays, the level of reproducibility was poor and a linear or logarithmic relationship did not exist
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Eissa, Dalia Essam, Engy Refaat Rashed, and Mostafa Essam Eissa. "Suitability System of Microbiological Method for Nystatin Potency Determination in the Routine Analysis Using Agar Diffusion Method." SciMedicine Journal 3, no. 4 (2021): 302–15. http://dx.doi.org/10.28991/scimedj-2021-0304-2.

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Nystatin is a polyene macrolide antifungal active which is used for the treatment of candidiasis and obtained from some species of Streptomycesbacteria. The present work describes the statistical suitability analysis for regular monitoring of the agar diffusion bioassay in a simple, inexpensive and time-saving process before potency determination. A balanced (symmetrical) two-dose parallel line assay model was applied using the agar well diffusion method for quantification of Nystatin in raw material and finished medicinal dosage form. The routine inspection methodology yielded good results an
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Gummuluri, Sriram, Venkata Teja Kavalipurapu, and Apoorva Vasundhara Kaligotla. "Antimicrobial efficacy of Novel Ethanolic Extract of Morinda Citrifolia Against Enterococcus Feacalis by Agar Well Diffusion Method and Minimal Inhibitory Concentration- An Invitro Study." Brazilian Dental Science 22, no. 3 (2019): 365–70. http://dx.doi.org/10.14295/bds.2019.v22i3.1731.

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Introduction: the long term success of root canal treatment is ultimately related to the effective debridement and disinfection of the root canal system. Hence, the irrigants play an important role in achieving the good penetrability and bactericidal activity. The present study was mainly aimed at evaluating the invitro antimicrobial efficacy of Novel Ethanolic Extract of Morinda Citrifolia by agar well diffusion and minimal inhibitory concentration. Materials and Methods:the antimicrobial efficacy of the Novel Ethanolic Extract of Morinda Citrifolia was tested using agar well diffusion and mi
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Nalini, T. J., H. N. Ramesh Babu, N. Rajeshwari, K. Y. Prathibha, and E. Keshamma. "Assay of Antimicrobial Activity of Sida acuta (Burm. F.) by Well Diffusion Method." International Journal of Current Microbiology and Applied Sciences 11, no. 9 (2022): 183–89. http://dx.doi.org/10.20546/ijcmas.2022.1109.021.

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Sida acuta is a shrub indigenous to pantropical regions. Sida acuta belongs to the mallow family, Malvaceae. The plant is widely used for its various pharmacological properties. Therefore, in the current study antifungal activity for leaf, stem and root extracts of Sida acuta were carried out. Ethanol (50%) was used successively for extraction of active principles from the dried powdered leaves, stem and root. The antifungal screening was done with two plant pathogens viz. Claviceps purpurea MTCC 2334 and Macrophemina phaseolina MTCC 10576 as test microorganisms. In the agar-well diffusion ass
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Dissertations / Theses on the topic "Agar well diffusion assay"

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Broetto, Sabrina Garcia. "Clonagem e análise da expressão de genes de proteínas de mamão papaia com atividade inibitória sobre poligalacturonases fúngicas." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/9/9131/tde-01102013-144633/.

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As proteínas inibidoras de poligalacturonases (PGIPs) presentes na parede celular são capazes de limitar o potencial destrutivo da poligalacturonase (PG) fúngica e, assim, constituem um tipo importante dentre os diversos sistemas de defesa do tecido vegetal frente à infecção fúngica. No mamão, o ataque fitopatogênico é o principal causador de danos pós-colheita, e sua alta susceptibilidade pode estar relacionada com a baixa eficácia ou pouca abundância dos meios de defesa anti-fitopatogênica. Uma vez que isso pode estar relacionado com as PGIPs e nada se conhece sobre o papel dessas proteínas
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Rodrigues, Manuela Luiza Toti. "Avaliação de métodos para testes de suscetibilidade in vitro de enxaguantes bucais frente a espécies do gênero Candida." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/60/60135/tde-17122008-185330/.

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A candidíase é uma infecção fúngica oportunista freqüente, causada por leveduras do gênero Candida. A utilização de anti-sépticos como medida complementar na higiene oral é cada vez mais difundida. Sendo assim, é de grande importância a avaliação das metodologias que são utilizadas para avaliação da atividade antifúngica. A avaliação in vitro da sensibilidade de leveduras a anti-sépticos tem sido pouco relatada e os resultados nem sempre são concordantes, pois as metodologias são divergentes. O objetivo desse estudo foi avaliar três metodologias: macro e microdiluição em caldo e difusão em ága
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Santos, Marcos Roberto dos. "DESENVOLVIMENTO E VALIDAÇÃO DE METODOLOGIAS ANALÍTICAS PARA DETERMINAÇÃO DO ITRACONAZOL MATÉRIA-PRIMA E CÁPSULAS." Universidade Federal de Santa Maria, 2008. http://repositorio.ufsm.br/handle/1/5890.

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Itraconazole is a triazole antifungical agent with a broad spectrum of activity belonging to the class of azole, indicated in the treatment of different types of mycotic infections systemic and local. Its mechanism of action is based on the ability to inhibition of synthesis of ergosterol which is a component of vital importance to the cell membrane of the fungi. This drug has only official methodology for raw material, described in British and European Pharmacopeia. In this paper, following the main guidelines for validation, were developed and validated quantitative analytical methods that c
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Whatley, Jenny J. (Jenny Johnson) 1982. "An in-vitro evaluation on the biocompatibility of resilon by the microbiota of the infected root canal utilizing an agar disc diffusion assay." Thesis, 2012. http://hdl.handle.net/1805/3093.

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Indiana University-Purdue University Indianapolis (IUPUI)<br>Resilon is a resin-based obturation material that claims to create a monoblock through bonding of RealSeal sealer to the dentin walls and to the core material. Resilon is comprised of a biodegradable polymer, polycaprolactone, and inorganic fillers. Resilon has been shown to undergo enzymatic hydrolysis by bacterial enzymes such as lipase. This study aims to demonstrate if bacteria found within the infected root canal system are capable of degrading Resilon utilizing an agar disc hydrolysis method. A 0.1-percent Resilon emulsion and
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Motara, Farhad Essop. "Determination of the antimicrobial properties of Withania somnifera and Xysmalobium undulatum plant tinctures in terms of the disc diffusion assay and the agar dilution sensitivity test." Thesis, 2007. http://hdl.handle.net/10321/326.

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Thesis (M.Tech.: Homoeopathy) - Dept. of Homoeopathy, Durban Institute of Technology, 2003 xviii, 122 leaves<br>This study was designed to investigate the antimicrobial efficacy of Withania somnifera and Xysmalobium undulatum plant tinctures in 62% ethanol in inhibiting the in vitro growth of Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus.
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Novotná, Lucie. "Teplotně závislá antimikrobiální aktivita bílkových proteinů u prekociálních a altriciálních druhů ptáků." Master's thesis, 2015. http://www.nusl.cz/ntk/nusl-343811.

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Microorganisms are considered to be crucial selective factor affecting reproductive success of birds. It is hypothesized that egg-white antimicrobial proteins and incubation behavior are the most important defense mechanisms that eliminates the risk of microbial trans-shell infection. The latest studies supposed that incubation temperatures may significantly affect the antimicrobial activity of egg white proteins. The concentration of egg white antimicrobial proteins as well as incubation patterns differ among altricial and precocial species of birds. However, experimental study testing the ef
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McCanna, David. "Development of Sensitive In Vitro Assays to Assess the Ocular Toxicity Potential of Chemicals and Ophthalmic Products." Thesis, 2009. http://hdl.handle.net/10012/4338.

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The utilization of in vitro tests with a tiered testing strategy for detection of mild ocular irritants can reduce the use of animals for testing, provide mechanistic data on toxic effects, and reduce the uncertainty associated with dose selection for clinical trials. The first section of this thesis describes how in vitro methods can be used to improve the prediction of the toxicity of chemicals and ophthalmic products. The proper utilization of in vitro methods can accurately predict toxic threshold levels and reduce animal use in product development. Sections two, three and four describe t
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Book chapters on the topic "Agar well diffusion assay"

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Hewitt, William. "The Agar Diffusion Assay – Its Quantitative Basis." In Microbiological Assay for Pharmaceutical Analysis. CRC Press, 2003. http://dx.doi.org/10.1201/b12428-2.

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Pasrija, Ritu, and Deepika Kumari. "Disk Diffusion Assay for Testing the Drug Sensitivity of Pathogenic Fungi in Agar." In Protocols for Studying Pathogenic Fungi. Springer Nature Singapore, 2025. https://doi.org/10.1007/978-981-96-7047-5_6.

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Corbett, Thomas H., Frederick A. Valeriote, Lisa Polin, et al. "Discovery of Solid Tumor Active Agents Using a Soft-Agar-Colony-Formation Disk-Diffusion-Assay." In Cytotoxic Anticancer Drugs: Models and Concepts for Drug Discovery and Development. Springer US, 1992. http://dx.doi.org/10.1007/978-1-4615-3492-1_3.

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Srivastava, Anmol, Vivek Kumar, and Vishnu Agarwal. "Antimicrobial Activity of Some Essential Oils Against Pseudomonas aeruginosa." In Proceedings of the Conference BioSangam 2022: Emerging Trends in Biotechnology (BIOSANGAM 2022). Atlantis Press International BV, 2022. http://dx.doi.org/10.2991/978-94-6463-020-6_4.

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AbstractThe emergence of multidrug resistance in bacteria due to overuse of antibiotics is becoming an important health concern in recent years, which requires development of novel alternatives to fight against these bacteria. Essential oils (EOs) are secondary metabolites that have different components and chemical compositions which may provide promising solution to the problem of rising number of drug resistant bacteria, as they can effectively kill bacteria. Here, in this study our aim is to determine the efficacy of lemongrass, rosemary, clary sage, geranium and tea tree essential oil aga
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Londonkar, Ramesh, and Maithilee Kesralikar. "In Vitro Anticancer Screening of Methanolic Extract of Stachytarpheta Mutabilis." In Proceedings of the Conference BioSangam 2022: Emerging Trends in Biotechnology (BIOSANGAM 2022). Atlantis Press International BV, 2022. http://dx.doi.org/10.2991/978-94-6463-020-6_19.

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AbstractIn therapeutic plants, phytochemicals are found in abundance. A family of verbena known as vervain (verbenaceae) consists of over 100 genera and nearly 2600 species. Among the Verbenaceae family is Stachytarpheta, a green perennial herb. The species is being examined in this study for its phytochemical, antioxidant, and anti-inflammatory properties. Stachytarpheta mutabilis methanol extracts were studied for their phytochemical screening, antimicrobial, anti-inflammatory, and anticancerous effects in the current study. Phytochemical study revealed the presence of alkaloids, flavonoids,
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Zamri, Amir Izzwan, Nor Hazwani Mohd Hasali, Muhammad Hariz Mohd Hasali, Tuan Zainazor Tuan Chilek, Fisal Ahmad, and Mohd Khairi Mohamed Zainol. "Microbiological Diversity and Properties of Stingless Bee Honey." In Advances in Environmental Engineering and Green Technologies. IGI Global, 2023. http://dx.doi.org/10.4018/978-1-6684-6265-2.ch008.

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The study was to compare and evaluate the performance of stingless bee honey (Heterotrigona itama spp.) with ordinary honey in terms of proximate composition as a comparison. Both honeys have shown diverse application and importance either traditionally and scientifically. However, due to the heightened interest on stingless bee honey, antimicrobial tests were also performed to determine the inhibition activity of stingless bee honey against food-borne pathogens using agar well diffusion assay. All three honey samples showed very good inhibitory activities (measured by inhibition zone) against
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HEWITT, WILLIAM, and STEPHEN VINCENT. "THE AGAR DIFFUSION ASSAY." In Theory and Application of Microbiological Assay. Elsevier, 1989. http://dx.doi.org/10.1016/b978-0-12-346445-3.50008-8.

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Tantray, Javeed Ahmad, Sheikh Mansoor, Rasy Fayaz Choh Wani, and Nighat Un Nissa. "Antibiotic resistance test by agar well diffusion method." In Basic Life Science Methods. Elsevier, 2023. http://dx.doi.org/10.1016/b978-0-443-19174-9.00042-8.

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Maitip, Jakkrawut, Sirikarn Sanpa, Michael Burgett, and Bajaree Chuttong. "Antimicrobial Activity From Five Species of Stingless Bee (Apidae meliponini) Honey From South East Asia (Thailand)." In Advances in Environmental Engineering and Green Technologies. IGI Global, 2023. http://dx.doi.org/10.4018/978-1-6684-6265-2.ch003.

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In Thailand, there have been limited investigations on the antibacterial properties of stingless bee honey. The purpose of this research is to investigate the physicochemical and antibacterial characteristics of five stingless bee species, including Lepidotrigona flavibasis, L. doipaensis, Lisotrigona furva, Tetragonula laeviceps species complex, and T. testaceitarsis complex from two geographical locations in Thailand: North (Chiang Mai) and Southeast (Chanthaburi). The moisture content from five species of stingless bee ranged from 27.6 to 32.0 g/100g. The range of pH in stingless bee honey
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Priya, K., M. Thenmozhi, S. Jayavarshini, and S. Varsha. "COMPARATIVE EVALUATION OF ANTIBACTERIAL EFFECTUAL CHARACTERISTICS OF TRIPLE HERBAL LEAF EXTRACTS AGAINST RESPIRATORY PATHOGENIC ISOLATES." In Futuristic Trends in Biotechnology Volume 3 Book 13. Iterative International Publishers, Selfypage Developers Pvt Ltd, 2024. http://dx.doi.org/10.58532/v3bjbt13p1ch8.

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The goal of the current study was to assess the antibacterial efficacy of herbal leaf aqueous extracts (Justicia adhatoda, Mukia maderaspatana, and Solanum triloblatum) against respiratory pathogenic isolates. The antibacterial activity of J. adhatoda's aqueous extract demonstrated a zone of inhibition of 14 mm, 15 mm, and 13 mm against E. coli, S. aureus, and K. pneumoniae, respectively, while M. maderaspatana leaf extract demonstrated a zone of inhibition of about 13 mm, 17 mm, and 21 mm against E. coli, S. aureus, and K. pneumoniae, respectively. Using the agar well diffusion method, the S.
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Conference papers on the topic "Agar well diffusion assay"

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AlBERMANI, Oruba K., Isrra Adnan Auda KHADHIM, and Nebras Mohammed SAHI. "ANTIMICROBIAL SUSCEPTIBILITY PATTERN AND THE ANTAGONISTIC EFFECT OF LACTOBACILLUS IN FIGHTING SHIGELLA SPP. ISOLATED FROM DIARRHEIC CHILDREN." In III.International Scientific Congress of Pure,Appliedand Technological Sciences. Rimar Academy, 2021. http://dx.doi.org/10.47832/minarcongress3-9.

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Background: Shigella is an major source of bacterial gastroenteritis in lack of health awareness in society. The handling of shigellos is mostly requires antibiotics. The using of probiotic of Lactic acid bacteria possess the counter effect against many dangerous bacterial pathogens which associated with gastroenteritis like Shigella spp. Aim: the purpose of this study to estimate the influence of lactic bacteria of the genus Lactobacillus on the population Shigella as a main pathogen involved in gastroenteritis in children. Patients and methods: A total of 50 stool specimens were collected du
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Happitiya, H. A. D. N. N., C. M. Nanayakkara, K. G. S. U. Ariyawansa, et al. "Antibacterial Activities of Lichen-associated Fungi in Mangrove Ecosystems in Sri Lanka as Potent Candidates for Novel Antibiotic Agents." In SLIIT International Conference on Advancements in Sciences and Humanities 2023. Faculty of Humanities and Sciences, SLIIT, 2023. http://dx.doi.org/10.54389/slzp7371.

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Antimicrobial resistance is a global threat to humans, prompting an increasing interest in exploring and developing novel antimicrobial substances derived from diverse sources. Together with the emergence of new diseases the search for novel drug leads has intensified. Less explored microbial habitats have become prime targets in mining for novel antimicrobial molecules. Secondary metabolites synthesized by lichen-associated fungi are good potential targets in this regard. Hence, this study was carried out to explore the antibacterial potential of lichen associated fungi in mangrove ecosystems
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Wang, Siqi, Colin P. McCoy, Gavin P. Andrews, Matthew Wylie, and Yi Ge. "Development of Antibacterial Carbon Dots by Using a One-Pot Microwave-Assisted Approach." In 5th World Conference on Chemistry and Chemical Engineering and 5th World Conference on Advanced Materials, Nanoscience and Nanotechnology. Eurasia Conferences, 2024. https://doi.org/10.62422/978-81-970328-7-5-018.

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Carbon dots (CDs), as emerging nanomaterials, have garnered significant attention for their exceptional properties including high biocompatibility, environmental friendliness, and versatile functionalization capabilities. These characteristics make CDs and carbon-dot-based nanomaterials promising candidates for various biomedical applications. In this study, we developed a novel microwave-assisted method to produce broad-spectrum antibacterial CDs, offering a cost-effective and green alternative to traditional synthesis. Using citric acid, ascorbic acid, tetraethylenepentamine, spermidine, and
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Saeed MOHAMMED, Luma. "STUDY THE ANTIBACTERIAL ACTIVITY OF ALCOHOLIC EXTRACT OF ALLIUM SATIVUM ON PSEUDOMONAS AERUGINOSA AND COMPARE WITH SOME ANTIBIOTICS." In V. International Scientific Congress of Pure, Applied and Technological Sciences. Rimar Academy, 2022. http://dx.doi.org/10.47832/minarcongress5-3.

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Twenty isolate of P.aerugiosa isolated from Baghdad hospitals identified morphologically and biochemical tests then vitek system.Disk diffusion method carried out to determined the sensitivity of bacterial isolates to different antibiotics like amoxicillin,ciprofloxacin, meropenem and cefotaxim by using Muller-Hinton agar, the results showed that 100% of isolates had resistance to amoxicillin,40% of resistance to cefotxim, 20% of resistance to ciprofloxacin and meropenem. Agar well method used to determined the minimal inhibitory concentrations for alcoholic extract of Allium sativum which pre
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Erza, Nurul Nadifa, Fajriati Zulfa, and Yuni Setyaningsih. "Antifungal Test of the Ethanol Extract of Brotowali Stem (Tinospora Crispa) on the Growth of Trichophyton Rubrum in Vitro." In The 7th International Conference on Public Health 2020. Masters Program in Public Health, Universitas Sebelas Maret, 2020. http://dx.doi.org/10.26911/the7thicph.05.02.

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ABSTRACT Background: Trichophyton rubrum is an infectious dermatophyte fungus which is the most common cause of dermatophytosis. Fungal resistance and the side effects of therapy are problems of antifungal agents. Phytochemical test of brotowali stem extract (Tinospora crispa) consist of flavonoids, phenols and triterpenoids which have antifungal effects. This study aimed to examine the effectiveness of brotowali stem extract (Tinospora crispa) as antifungal agent towards the growth of Trichophyton rubrum in vitro using agar well diffusion method. Subjects and Method: This was an experimental
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Kalyan, N. K., S. G. Lee, W.-T. Hum, R. Hartzell, M. Levner, and P. P. Hung. "IN VITRO STUDIES ON THE BINDING OF TISSUE-TYPE PLASMINOGEN ACTIVATOR (t-PA) AND UROKINASE (u-PA) TO LIVER MEMBRANES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643603.

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The plasminogen activators, t-PA and u-PA, are glycoproteins known to be involved in homeostasis of the blood clotting system, and thus are of potential clinical use in the treatment of thrombosis. Several in vivo studies have shown that both t-PA and u-PA are quickly removed from the blood circulation, predominantly by the liver. The mechanism by which the liver removes these proteins is not understood. To delineate this, we conducted in vitro studies of binding of PAs or their derivatives to isolated mouse liver membranes utilizing a functional assay developed in our laboratory. The assay co
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Sahoo, Debasish, Virendra Vaishnav, Tanushree Chatterjee, and Navita Gupta. "HERBAL DIETARY SUPPLEMENT – A MODERN APPROACH IN COMPLEMENTARY AND ALTERNATIVE MEDICINE (CAM) IN HEALTH CARE SCIENCE." In International Conference on Public Health and Medical Sciences. Goodwood Conferences, 2022. http://dx.doi.org/10.35912/icophmeds.v1i1.24.

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Preliminary pharmacological study of herbal based dietary supplement formulation based on extracts or whole plants derived from fruits, root, berries, macrofungus and leaves as a promising, safe and effective alternative to synthetic and pharmaceutical dietary supplements, in-vitro studies such as antibacterial, anti-oxidant and anti-inflammatory activity for extract of dietary supplements. Nutritional assessment of nutritional attributes as suggested by AOAC method, Phytochemical analysis by standard chemical procedures, Quantitative estimate Alkaloid, Flavonoid, Phenolic, Tannin. In-vitro st
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Bathany, Ce´dric, Thomas Suchyna, and Susan Z. Hua. "A Microfluidic Chip for Studying Intercellular Communication via Gap Junction Channels." In ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. ASMEDC, 2010. http://dx.doi.org/10.1115/nemb2010-13135.

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Cells in tissues and organs coordinate their activities with each other, and this communication is mainly mediated by specialized channels, called Gap Junctions. To date, our understanding of specificity of reagents and extracellular stimuli to gap junctions is very limited. Existing techniques for gap junctions assay are tedious and not convenient. We have developed a microfluidic chip that is capable of detecting chemical diffusion across gap junctions, as well as screening reagents for specific gap junction channels.
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Kumarasinghe, N. M. A., Charitha Thambiliyagodage, Madara Jayanetti, and Heshan Liyanaarachchi. "Antibacterial Activity of Zn Decorated TiO2 Nanocomposites." In SLIIT International Conference on Advancements in Sciences and Humanities 2023. Faculty of Humanities and Sciences, SLIIT, 2023. http://dx.doi.org/10.54389/usor2577.

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Bacterial infections have a significant public health impact. Infections are caused by bacteria in animals, plants as well as humans. Pathogenic bacteria can produce toxins, which are chemical poisons that interfere with cell function such as digestion of normal human enzymes, evasion of infection-fighting white blood cells, and immune clearance. Antibiotic prophylaxis is used to prevent bacterial infection. Antibiotic resistance is one of the most serious concerns in world health. Antibacterial nanoparticles are one possible answer to antimicrobial resistance. These nanomaterials not only kil
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Montana, Fajar Daniswara, Yuni Setyaningsih, and Fajriati Zulfa. "Effectiveness of Cocoa (Theobroma Cacao L.) Seed Extract on the Growth of in Vitro Malassezia Furfur." In The 7th International Conference on Public Health 2020. Masters Program in Public Health, Universitas Sebelas Maret, 2020. http://dx.doi.org/10.26911/the7thicph.05.01.

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ABSTRACT Background: Pityriasis versicolor or Tinea versicolor is a skin disease caused by the Malassezia furfur which is often found in Indonesia. People can use anti-fungal drugs to treat this disease. However, long-term use of anti-fungal drugs is relatively more expensive and can have side effects for its users. Cocoa bean husk contains flavonoids, saponins, and alkaloids which have anti-fungal effects. This study aimed to determine the antifungal effectiveness of the cocoa bean husk extract on the growth of M. furfur. Subjects and Methods: This was an experimental study using cocoa bean h
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Reports on the topic "Agar well diffusion assay"

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ปิยะธีรธิติวรกุล, สมเกียรติ, та ศิริรัตน์ เร่งพิพัฒน์. แลกติกแอซิดแบคทีเรียที่ใช้เป็นโพรไบโอติกสำหรับปลากะพงขาว Lates calcarifer : รายงานฉบับสมบูรณ์. ศูนย์เชี่ยวชาญเฉพาะทางด้านเทคโนโลยีชีวภาพทางทะเล ภาควิชาวิทยาศาสตร์ทางทะเล คณะวิทยาศาสตร์ จุฬาลงกรณ์มหาวิทยาลัย, 2005. https://doi.org/10.58837/chula.res.2005.76.

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การแยกแลกติกแอซิดแบคทีเรียจากทางเดินอาหารของปลากะพงขาว เพื่อคัดเลือกแบคทีเรียที่มีสมบัติเป็นโพรไบโอติก ด้วยเทคนิค well agar diffusion ผลพบว่า มี 5 ไอโซเลต (กำหนดให้เป็น LAB-1 - LAB-5) ที่สามารถยับยั้งการเจริญของ Aeromonas hydrophila แบคทีเรียก่อโรคในปลาได้ ผลการวิเคราะห์ปริมาณองค์ประกอบในอาหารผสมแบบเปียก พบว่า ปริมาณสารอาหารต่างๆ ในอาหารมีเพียงพอกับความต้องการของปลากะพงขาว ยกเว้นปริมาณไขมันที่ต่ำเกินไป ความเข้มข้นของ A. hydrophila ที่ทำให้ปลาตาย 50% (LC50) หลังจากที่ปลาได้รับเชื้อแล้ว 72 ชั่วโมง มีค่าเท่ากับ 7.76 log10 เซลล์/มล. และที่ 96 และ 120 ชั่วโมง มีค่าเท่ากับ 7.47 และ 7.26 log10 เซลล์/
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