Journal articles: 'Bovine herpesviruses'

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De-Giuli, Luciana, Simone Magnino, Pier Giorgio Vigo, Iris Labalestra, and Massimo Fabbi. "Development of a Polymerase Chain Reaction and Restriction Typing Assay for the Diagnosis of Bovine Herpesvirus 1, Bovine Herpesvirus 2, and Bovine Herpesvirus 4 Infections." Journal of Veterinary Diagnostic Investigation 14, no. 4 (2002): 353–56. http://dx.doi.org/10.1177/104063870201400417.

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A multiplex polymerase chain reaction (PCR) method coupled with a restriction analysis of PCR products (PCR with restriction fragment length polymorphism) was developed for the simultaneous detection of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections. The specificity, sensitivity, and practical diagnostic applicability of this method were evaluated. This assay may be also adapted to the diagnosis of suid herpesvirus 1 and equine herpesviruses 1 and 3 and could become a powerful diagnostic tool.

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BRAKE, F., and M. I. STUDDERT. "Molecular epidemiology and pathogenesis of ruminant herpesviruses including bovine, buffalo and caprine herpesviruses 1 and bovine encephalitis herpesvirus." Australian Veterinary Journal 62, no. 10 (1985): 331–34. http://dx.doi.org/10.1111/j.1751-0813.1985.tb07652.x.

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Collins, James K., Christa Bruns, Tracy L. Vermedahl, et al. "Malignant Catarrhal Fever: Polymerase Chain Reaction Survey for Ovine Herpesvirus 2 and Other Persistent Herpesvirus and Retrovirus Infections of Dairy Cattle and Bison." Journal of Veterinary Diagnostic Investigation 12, no. 5 (2000): 406–11. http://dx.doi.org/10.1177/104063870001200503.

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Using a polymerase chain reaction (PCR) test for sequences of ovine herpesvirus 2 (OHV2), this virus was shown to be significantly associated with sheep-associated malignant catarrhal fever (SA-MCF) in terminal cases of disease in 34 cattle and 53 bison. Ovine herpesvirus 2 was not detected in cattle (38) and bison (10) that succumbed to other diseases. Other persistent herpesviruses, retroviruses, and pestivirus, some of which have been previously isolated from cases of SA-MCF, were not associated with the disease. These included bovine herpesvirus 4 (BHV4), bovine lymphotrophic herpesvirus (BLHV), bovine syncytial virus (BSV, also known as bovine spumavirus), bovine immunodeficiency virus (BIV), and bovine viral diarrhea virus (BVDV). A PCR survey for OHV2 in DNA from individual cow's peripheral blood lymphocytes in 4 dairies showed that the 1 dairy that was in close contact to sheep had a prevalence of OHV2 of 21.3%, whereas the 3 other dairies had no OHV2. Prevalence of the other herpesviruses and retroviruses in the dairy cows was variable, ranging from 2% to 51% for BHV4, 52% to 78.7% for BLHV, and 10% to 34% for BSV Bovine lymphotrophic herpesvirus and BSV were also found in a few (1–4 of 21 tested) cases of terminal SA-MCF, but BIV and BVDV were not found in either the dairy cows sampled, or in the cases of SA-MCF. No significant correlation was found between the presence of any 2 viruses (OHV2, BHV4, BLHV, BSV) in the dairy cows or terminal cases of SA-MCF.

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Studdert, M. J., F. Brake, and G. F. Browning. "Bovine encephalitis herpesvirus is different from bovine herpesvirus 1 and from other ruminant herpesviruses." Australian Veterinary Journal 62, s1 (1985): 149–50. http://dx.doi.org/10.1111/j.1751-0813.1985.tb13935.x.

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Peshev, Raiko, Ivo Sirakov, Nedelcho Nedelchev, and Stoil Karadzhov. "ETIOLOGICAL AND MOLECULAR BIOLOGICAL INVESTIGATION OF CAPRINE HERPESVIRUS 1 ISOLATED IN BULGARIA." Archives of Veterinary Medicine 2, no. 2 (2009): 27–38. http://dx.doi.org/10.46784/e-avm.v2i2.216.

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From goat and bucks with genital disorders and abortions the attempts for isolation of caprine herpesvirus 1 (CHV 1) were carried out. For virus exaltation Dexametazone was used. For viruses isolation vaginal, nasal, rectal, preputial swabs and organ samples were used. Primary and continuous cell cultures rabbit kidney (RK), Madin Darby bovine kidney (MDBK), and embrional bovine trachea (EBTR) were used for cultivation. For determination of DNA type and lipid envelop 60 μg/ml iod desoxiuridine (JDUR) and the ether treat ment was used. Neutralization by specific hyperimmune serum obtained from Switzerland was performed. Five CHV 1 strains were isolated by cell cultures and identified as goat herpesviruses from dif ferent Bulgarian regions. After electron microscopy the viral agents with typical size and morphology for herpesviruses were established. For demonstration gC gene of CHV 1 the polymerase chain reaction (PCR) with primers designed from sequences deposited in gene bank were developed. Isolated on cell cultures herpesviruses were proved as caprine herpesvirus 1 by using applied PCR variant. The products after gC gene amplification from Bulgarian isolates were separated on the same place as the amplicons of ref er ence CHV 1 strains.

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Levy, Claire S., John Hopkins, George C. Russell, and Robert G. Dalziel. "Novel virus-encoded microRNA molecules expressed by ovine herpesvirus 2-immortalized bovine T-cells." Journal of General Virology 93, no. 1 (2012): 150–54. http://dx.doi.org/10.1099/vir.0.037606-0.

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A number of herpesviruses have now been shown to encode microRNAs (miRNAs) that have roles in control of both viral and cellular gene expression. Ovine herpesvirus 2 (OvHV-2) is the causative agent of sheep-associated malignant catarrhal fever, a fatal lymphoproliferative disease of cattle. Using massively parallel sequencing and Northern hybridization we have identified eight putative miRNAs encoded by OvHV-2 expressed in an OvHV-2-immortalized bovine lymphocyte cell line. These eight miRNAs are encoded in two areas of the OvHV-2 genome that contain no predicted protein coding regions and show no sequence similarity with other herpesvirus or cellular miRNAs. This represents the first report of the expression of virally encoded miRNAs in the genus Macavirus of herpesviruses.

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Muylkens, Benoît, Frédéric Farnir, François Meurens, et al. "Coinfection with Two Closely Related Alphaherpesviruses Results in a Highly Diversified Recombination Mosaic Displaying Negative Genetic Interference." Journal of Virology 83, no. 7 (2009): 3127–37. http://dx.doi.org/10.1128/jvi.02474-08.

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ABSTRACT Phylogenetic studies of the emergence and spread of natural recombinants in herpesviruses infecting humans and animals have been reported recently. However, despite an ever-increasing amount of evidence of recombination in herpesvirus history, the recombination process and the consequences on the genetic diversity of the progeny remain poorly characterized. We addressed this issue by using multiple single-nucleotide polymorphisms (SNPs) differentiating the two subtypes of an alphaherpesvirus, bovine herpesvirus 1 (BoHV-1). Analysis of a large sample of progeny virions obtained in a single growth cycle of coinfected BoHV-1 strains provided a prospective investigation of the recombination dynamics by using SNPs as recombination markers. We found that the simultaneous infection with two closely related herpesviruses results in a highly diversified recombination mosaic. From the analysis of multiple recombinants arising in the progeny, we provide the first evidence of genetic interference influencing the recombination process in herpesviruses. In addition, we report striking differences in the levels of recombination frequency observed along the BoHV-1 genome. With particular emphasis on the genetic structure of a progeny virus population rising in vitro, our data show to which extent recombination participates to the genetic diversification of herpesviruses.

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Okazaki, Katsunori, and Hiroshi Kida. "A synthetic peptide from a heptad repeat region of herpesvirus glycoprotein B inhibits virus replication." Journal of General Virology 85, no. 8 (2004): 2131–37. http://dx.doi.org/10.1099/vir.0.80051-0.

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Glycoprotein B (gB) is the most conserved glycoprotein of herpesviruses and plays important roles in virus infectivity. Two intervening heptad repeat (HR) sequences were found in the C-terminal half of all herpesvirus gBs analysed. A synthetic peptide derived from the HR region (aa 477–510) of bovine herpesvirus type 1 (BoHV-1) gB was studied for its ability to inhibit virus replication. The peptide interfered with cell-to-cell spread and consistently inhibited replication of BoHV-1, with a 50 % effective concentration value (EC50) of 5 μM. Inhibition of replication was obtained not only with herpesviruses including pseudorabies virus and herpes simplex virus type 1 but also partly with Newcastle disease virus. Possible mechanisms of membrane fusion inhibition by the peptide are discussed.

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MOLLEMA, L., M. C. M. DE JONG, and M. VAN BOVEN. "Prolonged persistence of bovine herpesvirus in small cattle herds: a model-based analysis." Epidemiology and Infection 133, no. 1 (2004): 137–48. http://dx.doi.org/10.1017/s0950268804003097.

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Herpesviruses can remain dormant in once-infected hosts and, upon reactivation, cause such hosts to become infectious. This phenomenon of latency and reactivation may enable herpesviruses to persist for a long time in small host populations. To quantify the effect of reactivation on persistence, the time to extinction of bovine herpesvirus type 1 (BHV-1) in small cattle populations was calculated. For realistic parameter values the mean time to extinction is already more than 100 years in a population of 10 animals. In a population of 20 animals the time to extinction is approximately 2000 years. The effects of vaccination on persistence were also studied, revealing that continued vaccination of the whole population could result in much faster eradication. For instance, in an isolated herd of 20 animals BHV-1 could be eradicated in 44 years.

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Costa, E. A., A. C. Vasconcelos, M. R. Q. Bomfim, et al. "Neurological disorder in cattle associated with bovine herpesvirus 4." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 63, no. 4 (2011): 828–35. http://dx.doi.org/10.1590/s0102-09352011000400006.

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A nested PCR assay was used to diagnose bovine encephalitis through herpesviruses including bovine herpesvirus 5 (BHV-5), bovine herpesvirus 1 (BHV-1), Aujeszky's disease virus (SHV-1), and ovine herpesvirus 2 (OHV-2) in 14 fragments of central nervous system (CNS) from cattle that died with neurological signs. In addition, as some samples of bovine herpesvirus type 4 (BHV-4) have been isolated from neural tissue, it was also tested by nested PCR. The cases of encephalitis occurred in isolation at different times of the year and did not present any seasonality. The duration of the clinical course ranged between 1 to 15 days, and in 64.3% of the cases it manifested between 1 to 2 days. The most frequently observed neurological signs were ataxia, recumbency, unsteadiness and inability to stand, opisthotonus, paddling movements, nystagmus and ptyalism. In the nested assay, there was no evidence of: BHV-1, SHV-1 or OHV-2 in the DNA obtained from the CNS in any of the samples. But the presence of BHV-4 was found in all fragments of the CNS in cattle which died presenting neurological signs. Moreover, BHV-5 was found in association with BHV-4 in two of these samples.

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Egyed, L. "Bovine Herpesvirus Type 4: A Special Herpesvirus (Review Article)." Acta Veterinaria Hungarica 48, no. 4 (2000): 501–13. http://dx.doi.org/10.1556/004.48.2000.4.13.

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This paper summarizes the history of and information on bovine herpesvirus type 4 (BoHV-4) from the first isolation to the most recent results. For almost twenty years BoHV-4 has been considered a typical herpes ‘orphan’ virus, which infects several species but causes no illness. The latest experiments revealed the close relationship of this virus with the immune system and other tissues. The virus was even considered as a possible candidate for a vector vaccine. BoHV-4 as a strange herpesvirus has several features which are not characteristic of other herpesviruses, such as several latency sites, persistence in serum, dividing cells necessary for virus replication, and the wide host range. In addition to describing the main features of the virion, replication, clinical signs, nomenclature problems, this review intends to concentrate on the new and strange results coming out from several laboratories worldwide. It is also suggested that because the virus combines several properties of various herpesvirus subfamilies and because of its close relationship with the immune system, it may deserve further attention as a representative of a potentially new genus within the Gammaherpesvirinae subfamily.

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Ehlers, Bernhard, and Stewart Lowden. "Novel herpesviruses of Suidae: indicators for a second genogroup of artiodactyl gammaherpesviruses." Journal of General Virology 85, no. 4 (2004): 857–62. http://dx.doi.org/10.1099/vir.0.79799-0.

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Five novel herpesviruses were identified in suid species from Africa (common warthog, Phacochoerus africanus) and South-East Asia (bearded pig, Sus barbatus; babirusa, Babyrousa babyrussa) by detection and analysis of their DNA polymerase genes. Three of the novel species, P. africanus cytomegalovirus 1, P. africanus lymphotropic herpesvirus 1 (PafrLHV-1) and S. barbatus lymphotropic herpesvirus 1 (SbarLHV-1), were closely related to known beta- (porcine cytomegalovirus) and gammaherpesviruses [porcine lymphotropic herpesvirus (PLHV) 1 and 3] of domestic pigs. In contrast, two novel species, S. barbatus rhadinovirus 1 (SbarRHV-1) and Babyrousa babyrussa rhadinovirus 1 (BbabRHV-1), were more closely related to a ruminant gammaherpesvirus, bovine herpesvirus 4 (BoHV-4), than to the porcine gammaherpesviruses PLHV-1, -2, -3, PafrLHV-1 and SbarLHV-1. SbarRHV-1, BbabRHV-1 and BoHV-4 were therefore tentatively assigned to a novel genogroup of artiodactyl gammaherpesviruses. This latter genogroup may also contain an as yet undiscovered gammaherpesvirus of domestic pigs, thereby adding a concern to their use in xenotransplantation.

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Hassan, Sherif T. S. "Shedding Light on the Effect of Natural Anti-Herpesvirus Alkaloids on SARS-CoV-2: A Treatment Option for COVID-19." Viruses 12, no. 4 (2020): 476. http://dx.doi.org/10.3390/v12040476.

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The whole world is currently facing an unseen enemy, called coronavirus disease 2019 (COVID-19), which is causing a global pandemic. This disease is caused by a novel single-stranded enveloped RNA virus, known as the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). Although huge efforts are being made to produce effective therapies to combat this disease, it continues to be one of the greatest challenges in medicine. There is no doubt that herpesviruses are one of the most important viruses that infect humans and animals, and infections induced by these pathogens have developed into a great threat to public health. According to the currently available evidence, the correlation between herpesviruses and coronaviruses is limited to the induced complications following the infections. For instance, the inflammation that is induced at the sites of infection could tie these viruses to each other in a relationship. Another example, bovine herpesvirus 1, which is an important pathogen of cattle, can cause a severe respiratory infection; the same way in which SARS-CoV-2 affects humans. Considering the current circumstances related to the COVID-19 crisis, this editorial paper, which belongs to the Special Issue “Recent Advances in Herpesviruses Research: What’s in the Pipeline?” aims to draw attention to some natural anti-herpesvirus alkaloid compounds, which have recently been proven to have excellent inhibitory efficacy against SARS-CoV-2 replication. Thus, this special focus is an attempt to hunt down various treatment options to combat COVID-19 based on repurposing drugs that are known to have multiple antiviral properties, including against herpesvirus.

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Delhon, G., M. P. Moraes, Z. Lu, et al. "Genome of Bovine Herpesvirus 5." Journal of Virology 77, no. 19 (2003): 10339–47. http://dx.doi.org/10.1128/jvi.77.19.10339-10347.2003.

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ABSTRACT Here we present the complete genomic sequence of bovine herpesvirus 5 (BHV-5), an alphaherpesvirus responsible for fatal meningoencephalitis in cattle. The 138,390-bp genome encodes 70 putative proteins and resembles the α2 subgroup of herpesviruses in genomic organization and gene content. BHV-5 is very similar to BHV-1, the etiological agent of infectious bovine rhinotracheitis, as reflected by the high level of amino acid identity in their protein repertoires (average, 82%). The highest similarity to BHV-1 products (≥95% amino acid identity) is found in proteins involved in viral DNA replication and processing (UL5, UL15, UL29, and UL39) and in virion proteins (UL14, UL19, UL48, and US6). Among the least conserved (≤75%) are the homologues of immediate-early (IE) proteins BICP0, BICP4, and BICP22, the three proteins being longer in BHV-5 than in BHV-1. The structure of the BHV-5 latency-related (LR) region departs markedly from that of BHV-1 in both coding and transcriptional regulatory regions. Given the potential significance of IE genes and the LR region in virus-neuron interactions, it is likely these differences contribute to BHV-5 neuropathogenicity.

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Raggo, Camilo, Monique Habermehl, Lorne A. Babiuk та Philip Griebel. "The in vivo effects of recombinant bovine herpesvirus-1 expressing bovine interferon-γ". Journal of General Virology 81, № 11 (2000): 2665–73. http://dx.doi.org/10.1099/0022-1317-81-11-2665.

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To study the biological relevance of using bovine herpesvirus-1 (BHV-1) as a vector for expressing cytokines, a BHV-1 virus that expressed bovine interferon-γ (IFN-γ) was constructed. This recombinant virus (BHV-1/IFNγ) was then used to infect the natural host in a respiratory disease model. In vitro characterization of the recombinant interferon-γ confirmed that the cytokine expressed in BHV-1-infected cells was biologically active. The in vivo effects of the recombinant IFN-γ were then analysed during a primary infection and after reactivation of a latent infection. During the primary infection, similar body temperature, clinical responses and virus shedding were observed for calves infected with either recombinant BHV-1/IFNγ or parental gC−/LacZ+ virus. An analysis of cellular and humoral responses did not reveal any significant immunomodulation by BHV-1/IFNγ during the primary infection. The stability and activity of recombinant IFN-γ was also analysed following the establishment of a latent infection. The presence of recombinant IFN-γ did not significantly alter virus shedding following reactivation. The isolation of reactivated BHV-1/IFNγ virus confirmed that a functional IFN-γ gene was retained during latency. Thus, herpesviruses may provide virus vectors that retain functional genes during latency and recrudescence.

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Meurens, François, Günther M. Keil, Benoît Muylkens, et al. "Interspecific Recombination between Two Ruminant Alphaherpesviruses, Bovine Herpesviruses 1 and 5." Journal of Virology 78, no. 18 (2004): 9828–36. http://dx.doi.org/10.1128/jvi.78.18.9828-9836.2004.

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ABSTRACT Homologous recombination between different species of alphaherpesviruses has been described between herpes simplex viruses 1 and 2 but has not yet been observed between other alphaherpesviruses. In the present study we chose to assess to what extent in vitro recombination can occur between members of a well-defined group of closely related viruses such as ruminant alphaherpesviruses. At 24 h after infection of epithelial bovine kidney cells with a double-deleted mutant of bovine herpesvirus 1 (BoHV-1) (containing green fluorescent protein and red fluorescent protein genes) and different ruminant alphaherpesviruses, four types of progeny viruses were detected and distinguished according to their phenotype. Frequent recombination events between identical or different strains of BoHV-1 were observed (up to 30%), whereas only two BoHV-1/BoHV-5 recombinants were identified, and no recombinants between BoHV-1 and less closely related caprine and cervine herpesviruses were detected. Restriction analysis of the genomes of the two BoHV-1/BoHV-5 recombinants showed different genetic backgrounds. One possessed a restriction pattern close to BoHV-1, whereas the other one was close to BoHV-5. This exhaustive analysis of each combination of coinfection in a unique situation of five closely related alphaherpesviruses revealed the importance of a high degree of genetic relatedness and similar parental virus growth kinetics for successful interspecific recombination.

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Desrosiers, R. C., J. Kamine, A. Bakker, et al. "Synthesis of bovine growth hormone in primates by using a herpesvirus vector." Molecular and Cellular Biology 5, no. 10 (1985): 2796–803. http://dx.doi.org/10.1128/mcb.5.10.2796.

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A strain of herpesvirus saimiri containing a bovine growth hormone (bGH) gene under the control of the simian virus 40 (SV40) late-region promoter was constructed. This strain, bGH-Z20, was replication competent and stably harbored the bGH gene upon serial passage. Nonpermissive marmoset T cells persistently infected with bGH-Z20 produced a 0.9-kilobase RNA which contained all of the bGH exon sequences and appeared to initiate within the SV40 promoter region. However, in permissively infected owl monkey kidney cells, RNAs containing growth hormone sequences appeared to initiate from herpesvirus saimiri promoters positioned upstream from the SV40-growth hormone gene. Persistently infected T cells in culture secreted 500 ng of bGH protein per 10(6) cells per 24 h during the several months of testing. The secreted protein was 21 kilodaltons, the size of authentic bGH. New World primates experimentally infected with bGH-Z20 produced circulating bGH and developed immunoglobulin G antibodies directed against bGH. Because herpesviruses characteristically remain latent in the infected host, these observations suggest a means for replacing gene products missing or defective in hereditary genetic disorders.

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Desrosiers, R. C., J. Kamine, A. Bakker, et al. "Synthesis of bovine growth hormone in primates by using a herpesvirus vector." Molecular and Cellular Biology 5, no. 10 (1985): 2796–803. http://dx.doi.org/10.1128/mcb.5.10.2796-2803.1985.

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A strain of herpesvirus saimiri containing a bovine growth hormone (bGH) gene under the control of the simian virus 40 (SV40) late-region promoter was constructed. This strain, bGH-Z20, was replication competent and stably harbored the bGH gene upon serial passage. Nonpermissive marmoset T cells persistently infected with bGH-Z20 produced a 0.9-kilobase RNA which contained all of the bGH exon sequences and appeared to initiate within the SV40 promoter region. However, in permissively infected owl monkey kidney cells, RNAs containing growth hormone sequences appeared to initiate from herpesvirus saimiri promoters positioned upstream from the SV40-growth hormone gene. Persistently infected T cells in culture secreted 500 ng of bGH protein per 10(6) cells per 24 h during the several months of testing. The secreted protein was 21 kilodaltons, the size of authentic bGH. New World primates experimentally infected with bGH-Z20 produced circulating bGH and developed immunoglobulin G antibodies directed against bGH. Because herpesviruses characteristically remain latent in the infected host, these observations suggest a means for replacing gene products missing or defective in hereditary genetic disorders.

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Neurath, AR, N. Strick та Y.-Y. Li. "3-Hydroxyphthaloyl β-Lactoglobulin. III. Antiviral Activity against Herpesviruses". Antiviral Chemistry and Chemotherapy 9, № 2 (1998): 177–84. http://dx.doi.org/10.1177/095632029800900209.

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The spread of sexually transmitted diseases, including human immunodeficiency virus type 1 (HIV-1) and herpesvirus infections, has continued unabated despite educational efforts spearheaded as a response to the HIV-1 epidemic. This suggests the need for prophylactic measures, including the application of topical antiviral agents. Chemical modification of bovine β-lactoglobulin (β-LG), the major protein of whey, by hydroxyphthalic anhydride (3HP) led to the generation of a potent HIV-1 inhibitor (designated 3HP-β-LG) shown to also have activity against herpes simplex virus types 1 and 2 (HSV-1, HSV-2). This report provides more detailed results concerning the anti-herpesvirus activity of 3HP-β-LG, indicating that this compound: (i) inhibited infection by human cytomegalovirus (HCMV), which is known to besexually transmitted; (ii) inactivated the infectivity of both HSV-1 and HSV-2; (iii) inhibited cell-to-cell transmission of HSV-1 and HSV-2; and (iv) bound to HSV-1, HSV-2 and HCMV virus particles and partially inhibited the binding of anti-glycoprotein E (gE) and anti-gC monoclonal antibodies to HSV-1 and HSV-2. The binding of 3HP-β-LG to the herpesviruses under study was inhibited by aggregated human IgG, suggesting that the respective viral Fc receptor is one of the target sites for 3HP-β-LG. In agreement with results on inhibition of HIV-1 infection, 3HP-β-LG appears to be the acid anhydride-modified protein of choice as an antiviral agent against herpesviruses.

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Denner, Joachim. "Porcine Lymphotropic Herpesviruses (PLHVs) and Xenotranplantation." Viruses 13, no. 6 (2021): 1072. http://dx.doi.org/10.3390/v13061072.

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Porcine lymphotropic herpesviruses -1, -2 and -3 (PLHV-1, PLHV-2 and PLHV-3) are gammaherpesviruses which are widespread in pigs. They are closely related to the Epstein–Barr virus (EBV) and Kaposi sarcoma herpesvirus, both of which cause severe diseases in humans. PLHVs are also related to bovine and ovine gammaherpesviruses, which are apathogenic in the natural host, but cause severe diseases after transmission into other species. Until now, no association between PLHVs and any pig diseases had been described. However, PLHV-1 causes a post-transplantation lymphoproliferative disorder (PTLD) after experimental transplantations in minipigs. This disorder is similar to human PTLD, a serious complication of solid human organ transplantation linked to EBV. Xenotransplantation using pig cells, tissues and organs is under development in order to alleviate the shortage of human transplants. Meanwhile, remarkable survival times of pig xenotransplants in non-human primates have been achieved. In these preclinical trials, another pig herpesvirus, the porcine cytomegalovirus (PCMV), a roseolovirus, was shown to significantly reduce the survival time of pig xenotransplants in baboons and other non-human primates. Although PLHV-1 was found in genetically modified donor pigs used in preclinical xenotransplantation, it was, in contrast to PCMV, not transmitted to the recipient. Nevertheless, it seems important to use PLHV-free donor pigs in order to achieve safe xenotransplantation.

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Wild, Peter, Elisabeth M. Schraner, Jeanne Peter, Eva Loepfe, and Monika Engels. "Novel Entry Pathway of Bovine Herpesvirus 1 and 5." Journal of Virology 72, no. 12 (1998): 9561–66. http://dx.doi.org/10.1128/jvi.72.12.9561-9566.1998.

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ABSTRACT Herpesviruses enter cells by a yet poorly understood mechanism. We visualized the crucial steps of the entry pathway of bovine herpesvirus 1 (BHV-1) and BHV-5 by transmission and scanning electron microscopy, employing cryotechniques that include time monitoring, ultrarapid freezing, and freeze substitution of cultured cells inoculated with virus. A key step in the entry pathway of both BHV-1 and BHV-5 is a unique fusion of the outer phospholipid layer of the viral envelope with the inner layer of the plasma membrane and vice versa resulting in “crossing” of the fused membranes and in partial insertion of the viral envelope into the plasma membrane. The fusion area is proposed to function as an axis for driving the virus particle into an invagination that is concomitantly formed close to the fusion site. The virus particle enters the cytoplasm through the opened tip of the invagination, and the viral envelope defuses from the plasma membrane. There is strong evidence that the intact virus particle is then transported to the nuclear region.

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Mahony, Timothy J., Fiona M. McCarthy, Jennifer L. Gravel, Lani West, and Peter L. Young. "Construction and Manipulation of an Infectious Clone of the Bovine Herpesvirus 1 Genome Maintained as a Bacterial Artificial Chromosome." Journal of Virology 76, no. 13 (2002): 6660–68. http://dx.doi.org/10.1128/jvi.76.13.6660-6668.2002.

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ABSTRACT The complete genome of bovine herpesvirus 1 (BoHV-1) strain V155 has been cloned as a bacterial artificial chromosome (BAC). Following electroporation into Escherichia coli strain DH10B, the BoHV-1 BAC was stably propagated over multiple generations of its host. BAC DNA recovered from DH10B cells and transfected into bovine cells produced a cytopathic effect which was indistinguishable from that of the parent virus. Analysis of the replication kinetics of the viral progeny indicated that insertion of the BAC vector into the thymidine kinase gene did not affect viral replication. Specific manipulation of the BAC was demonstrated by deleting the gene encoding glycoprotein E by homologous recombination in DH10B cells facilitated by GET recombination. These studies illustrate that the propagation and manipulation of herpesviruses in bacterial systems will allow for rapid and accurate characterization of BoHV-1 genes. In turn, this will allow for the full utilization of BoHV-1 as a vaccine vector.

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Kálmán, Dóra, and László Egyed. "PCR DETECTION OF BOVINE HERPESVIRUSES FROM NONBOVINE RUMINANTS IN HUNGARY." Journal of Wildlife Diseases 41, no. 3 (2005): 482–88. http://dx.doi.org/10.7589/0090-3558-41.3.482.

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STUDDERT, MJ. "A brief review of studies of bovine and equine herpesviruses." Australian Veterinary Journal 66, no. 12 (1989): 401–2. http://dx.doi.org/10.1111/j.1751-0813.1989.tb13558.x.

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Goltz, Michael, Hermann Broll, Annette Mankertz, et al. "Glycoprotein B of bovine herpesvirus type 4: Its phylogenetic relationship to gB equivalents of the herpesviruses." Virus Genes 9, no. 1 (1994): 53–59. http://dx.doi.org/10.1007/bf01703435.

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Schynts, Frédéric, François Meurens, Bruno Detry, Alain Vanderplasschen, and Etienne Thiry. "Rise and Survival of Bovine Herpesvirus 1 Recombinants after Primary Infection and Reactivation from Latency." Journal of Virology 77, no. 23 (2003): 12535–42. http://dx.doi.org/10.1128/jvi.77.23.12535-12542.2003.

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ABSTRACT Recombination is thought to be an important source of genetic variation in herpesviruses. Several studies, performed in vitro or in vivo, detected recombinant viruses after the coinoculation of two distinguishable strains of the same herpesvirus species. However, none of these studies investigated the evolution of the relative proportions of parental versus recombinant progeny populations after coinoculation of the natural host, both during the excretion and the reexcretion period. In the present study, we address this by studying the infection of cattle with bovine herpesvirus 1 (BoHV-1). The recombination of two BoHV-1 mutants lacking either glycoprotein C (gC−/gE+) or E (gC+/gE−) was investigated after inoculation of cattle by the natural route of infection. The results demonstrated that (i) recombination is a frequent event in vivo since recombinants (gC+/gE+ and gC−/gE−) were detected in all coinoculated calves, (ii) relative proportions of progeny populations evolved during the excretion period toward a situation where two populations (gC+/gE+ and gC−/gE+) predominated without fully outcompeting the presence of the two other detected populations (gC+/gE− and gC−/gE−), and (iii) after reactivation from latency, no gC+/gE− and gC−/gE− progeny viruses were detected, although gC+/gE− mutants, when inoculated alone, were detected after reactivation treatment. In view of these data, the importance of gE in the biology of BoHV-1 infection and the role of recombination in herpesvirus evolution are discussed.

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Bhat, M. Narayana, R. Manickam, and K. Kumanan. "Serological Evidence of Bovine Herpesviruses 1 and 2 in Asian Elephants." Journal of Wildlife Diseases 33, no. 4 (1997): 919–20. http://dx.doi.org/10.7589/0090-3558-33.4.919.

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Holz, Carine Lidiane, Samuel Paulo Cibulski, Thais Fumaco Teixeira, et al. "Serum neutralization with different types and subtypes of bovine herpesvirus 1 and 5." Pesquisa Veterinária Brasileira 30, no. 7 (2010): 515–22. http://dx.doi.org/10.1590/s0100-736x2010000700001.

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The serum neutralization (SN) test is the gold standard method to measure neutralizing antibodies to bovine herpesviruses. However, in view of the further subdivisions of bovine herpesviruses in types/subtypes, defining which virus to use at challenge in SN tests may be difficult. In view of that, this study was carried out to re-evaluate (SN) sensitivity with different types/subtypes of bovine herpesviruses types 1 (BoHV-1) and 5 (BoHV-5) as challenge viruses. Bovine sera (n=810) were collected from two distinct geographic regions and tested by SN with three type 1 viruses (BoHV-1.1 strains "Los Angeles" and "EVI123/98"; BoHV-1.2a strain "SV265/96") and three type 5 viruses (BoHV-5a strain "EVI88/95"; BoHV-5b strain "A663" and BoHV-5c "ISO97/95"). SN tests were performed with a 1 hour incubation of the serum-virus mixtures at 37ºC against 100 TCID50 of each of the viruses. SN sensitivity varied greatly depending on the challenge virus used in the test. The highest sensitivity (327 positive/810 total sera tested; 40.37%) was attained when the positive results to the six viruses were added together. No association could be found between any particular type or subtype of virus and the sensitivity of the test. When positive results to each single strain were considered, SN sensitivity varied from 41.7% to 81.7%, depending on the virus and the geographic region of origin of the sera. Variation was detected even when challenge viruses belonged to the same subtype, where disagreement between positive results reached 41%. These results indicate that one hour incubation SN tests against single viruses, as performed here, may display a significantly low sensitivity (p=0.05); performing SN tests against a number of different viruses may increase considerably SN sensitivity. Furthermore, the choice of virus used for challenge is critical in SN tests. In addition, sera from different geographic regions may give rise to disagreeing results with different strains of BoHV-1 and BoHV-5. This might be particularly relevant for control programs and in international trade, were maximum sensitivity should be targeted.

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Ren, Xiaodi, Jerome S. Harms, and Gary A. Splitter. "Tyrosine Phosphorylation of Bovine Herpesvirus 1 Tegument Protein VP22 Correlates with the Incorporation of VP22 into Virions." Journal of Virology 75, no. 19 (2001): 9010–17. http://dx.doi.org/10.1128/jvi.75.19.9010-9017.2001.

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ABSTRACT Tyrosine phosphorylation has been shown to play a role in the replication of several herpesviruses. In this report, we demonstrate that bovine herpesvirus 1 infection triggered tyrosine phosphorylation of proteins with molecular masses similar to those of phosphorylated viral structural proteins. One of the tyrosine-phosphorylated viral structural proteins was the tegument protein VP22. A tyrosine 38-to-phenylalanine mutation totally abolished the phosphorylation of VP22 in transfected cells. However, construction of a VP22 tyrosine 38-to-phenylalanine mutant virus demonstrated that VP22 was still phosphorylated but that the phosphorylation site may change to the C terminus rather than be in the N terminus as in wild-type VP22. In addition, the loss of VP22 tyrosine phosphorylation correlated with reduced incorporation of VP22 compared to that of envelope glycoprotein D in the mutant viruses but not with the amount of VP22 produced during virus infection. Our data suggest that tyrosine phosphorylation of VP22 plays a role in virion assembly.

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Šudomová, Miroslava, and Sherif T. S. Hassan. "Nutraceutical Curcumin with Promising Protection against Herpesvirus Infections and Their Associated Inflammation: Mechanisms and Pathways." Microorganisms 9, no. 2 (2021): 292. http://dx.doi.org/10.3390/microorganisms9020292.

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Herpesviruses are DNA viruses that infect humans and animals with the ability to induce latent and lytic infections in their hosts, causing critical health complications. The enrolment of nutraceutical anti-herpesvirus drugs in clinical investigations with promising levels of reduced resistance, free or minimal cellular toxicity, and diverse mechanisms of action might be an effective way to defeat challenges that hurdle the progress of anti-herpesvirus drug development, including the problems with drug resistance and recurrent infections. Therefore, in this review, we aim to hunt down all investigations that feature the curative properties of curcumin, a principal bioactive phenolic compound of the spice turmeric, in regard to various human and animal herpesvirus infections and inflammation connected with these diseases. Curcumin was explored with potent antiherpetic actions against herpes simplex virus type 1 and type 2, human cytomegalovirus, Kaposi’s sarcoma-associated herpesvirus, Epstein–Barr virus, bovine herpesvirus 1, and pseudorabies virus. The mechanisms and pathways by which curcumin inhibits anti-herpesvirus activities by targeting multiple steps in herpesvirus life/infectious cycle are emphasized. Improved strategies to overcome bioavailability challenges that limit its use in clinical practice, along with approaches and new directions to enhance the anti-herpesvirus efficacy of this compound, are also reviewed. According to the reviewed studies, this paper presents curcumin as a promising natural drug for the prevention and treatment of herpesvirus infections and their associated inflammatory diseases.

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Ashbaugh, Scott E., Karin E. Thompson, Ellen B. Belknap, Patricia C. Schultheiss, Shafiqul Chowdhury, and James K. Collins. "Specific Detection of Shedding and Latency of Bovine Herpesvirus 1 and 5 using a Nested Polymerase Chain Reaction." Journal of Veterinary Diagnostic Investigation 9, no. 4 (1997): 387–94. http://dx.doi.org/10.1177/104063879700900408.

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A sensitive method for simultaneously detecting and discriminating between bovine herpesviruses types 1 and 5 (BHV-1 and BHV-5) was developed using a nested polymerase chain reaction (PCR) technique. Following amplification using type-common primers derived from gC sequences, amplification using type-specific nesting primers produced different-sized bands specific to the corresponding types, as demonstrated by blot hybridization. Less than 0.1 plaque-forming units (PFU) of each virus and 75 fg or less of viral DNA were routinely detected. The PCR technique amplified correct product from 4 BHV-5 isolates and from 48 BHV-1 isolates, all from the United States, and did not amplify heterologous herpesviruses. The PCR technique was more sensitive than virus isolation in detection of BHV-1 or BHV-5 in nasal secretions from experimentally and naturally infected calves, and it detected BHV-1 or BHV-5 in trigeminal ganglia from these calves.

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Bartha, Adorjan, Abdalla M. Fadol, Heinrich Liebermann, et al. "Problems Concerning the Taxonomy of the ‘Movar-Type’ Bovine Herpesviruses." Intervirology 28, no. 1 (1987): 1–7. http://dx.doi.org/10.1159/000149991.

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Engels, Monika, Monica Palatini, Alfred E. Metzler, Urs Probst, Ueli Kihm, and Mathias Ackermann. "Interactions of bovine and caprine herpesviruses with the natural and the foreign hosts." Veterinary Microbiology 33, no. 1-4 (1992): 69–78. http://dx.doi.org/10.1016/0378-1135(92)90036-s.

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Wu, S. X., X. P. Zhu, and G. J. Letchworth. "Bovine Herpesvirus 1 Glycoprotein M Forms a Disulfide-Linked Heterodimer with the UL49.5 Protein." Journal of Virology 72, no. 4 (1998): 3029–36. http://dx.doi.org/10.1128/jvi.72.4.3029-3036.1998.

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ABSTRACT Nine glycoproteins (gB, gC, gD, gE, gG, gH, gI, gK, and gL) have been identified in bovine herpesvirus 1 (BHV-1). gM has been identified in many other alpha-, beta-, and gammaherpesviruses, in which it appears to play a role in membrane penetration and cell-to-cell fusion. We sought to express BHV-1 open reading frame UL10, which encodes gM, and specifically identify the glycoprotein. We corrected a frameshift error in the published sequence and used the corrected sequence to design coterminal peptides from the C terminus. These were expressed as glutathione S-transferase fusion proteins inEscherichia coli. The fusion protein containing the 63 C-terminal amino acids from the corrected gM sequence engendered antibodies that immunoprecipitated a 30-kDa protein from in vitro translation reactions programmed with the UL10 gene. Proteins immunoprecipitated by this antibody from virus-infected cells ran at 36 and 43 kDa in reducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and 43 and 48 kDa in nonreducing SDS-PAGE. Only the larger of the pair was present in virions. A 7-kDa protein was released from gM by reducing agents. The 7-kDa protein was not recognized in Western blots probed with the anti-gM antibody but reacted specifically with antibodies prepared against BHV-1 UL49.5, previously reported to be a 9-kDa protein associated with an unidentified 39-kDa protein (X. Liang, B. Chow, C. Raggo, and L. A. Babiuk, J. Virol. 70:1448–1454, 1996). This is the first report of a small protein covalently bound to any herpesvirus gM. Similar patterns of hydrophobic domains and cysteines in all known gM and UL49.5 homologs suggest that these two proteins may be linked by disulfide bonds in all herpesviruses.

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Delhon, Gustavo A., Marcelo J. González, and Pablo R. Murcia. "Susceptibility of sensory neurons to apoptosis following infection by bovine herpesvirus type 1." Journal of General Virology 83, no. 9 (2002): 2257–67. http://dx.doi.org/10.1099/0022-1317-83-9-2257.

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Like other members of the alpha subfamily of herpesviruses, bovine herpesvirus type 1 (BHV-1) establishes latent infections in sensory neurons. BHV-1 induces apoptosis in lymphoid cells in vivo and in epithelial cell lines, but the ability of BHV-1 to induce apoptosis in sensory neurons remains unknown. In this report, the susceptibility of rabbit ganglionic neurons to infection by BHV-1 was examined in vitro and in vivo. Following infection of cultured neurons with BHV-1, hallmarks of apoptosis such as chromatin condensation, DNA fragmentation and membrane blebbing were detected. The appearance of these changes was preceded by active viral DNA replication as determined by in situ hybridization. When viral DNA replication was blocked by treatment of cultures with an inhibitor of eukaryotic DNA polymerases, apoptosis but not virus attachment to neurons or bICP0 gene expression was completely prevented. Taken together, these results demonstrate that sensory neurons are not intrinsically resistant to BHV-1-induced apoptosis and that viral DNA replication plays a role in triggering the apoptotic programme. Infection of rabbits with BHV-1 resulted in pathological changes in the trigeminal ganglia (TG) which included mononuclear cell infiltration and neuronophagia. Morphological evidence of apoptosis was not detected in neurons, even in cells with advanced cytophatology. Furthermore, whereas DNA fragmentation was common in infiltrating cells, it was very rare and sporadic in neurons. Therefore, mechanisms in the TG should exist to prevent neuronal apoptosis upon BHV-1 infection.

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Lomonte, P., M. Bublot, P. P. Pastoret, and E. Thiry. "Location and characterization of the bovine herpesvirus type 4 thymidine kinase gene; comparison with thymidine kinase genes of other herpesviruses." Archives of Virology 127, no. 1-4 (1992): 327–37. http://dx.doi.org/10.1007/bf01309595.

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Burucúa, M. M., S. E. Pérez, A. C. Odeón, E. R. Cobo, S. Quintana, and M. S. Marin. "Cathelicidin bovine myeloid antimicrobial peptide (BMAP) 28 is involved in the inflammatory response against alpha-herpesviruses in the bovine nervous system." Molecular Immunology 122 (June 2020): 148–55. http://dx.doi.org/10.1016/j.molimm.2020.04.017.

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Cardoso, Tereza C., Ana Carolina G. Rosa, Helena L. Ferreira, et al. "Bovine herpesviruses induce different cell death forms in neuronal and glial-derived tumor cell cultures." Journal of NeuroVirology 22, no. 6 (2016): 725–35. http://dx.doi.org/10.1007/s13365-016-0444-5.

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Van Aerschot, Arthur, Guy Schepers, Roger Busson, et al. "Ribavirin Derivatives with a Hexitol Moiety: Synthesis and Antiviral Evaluation." Antiviral Chemistry and Chemotherapy 14, no. 1 (2003): 23–30. http://dx.doi.org/10.1177/095632020301400102.

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Current standard therapy for the treatment of chronic infections with hepatitis C virus consists of combination therapy with (pegylated) interferon-α and ribavirin. 1,5-Anhydrohexitol nucleoside analogues are constrained congeners known to mimic the ribonucleoside conformation. Within this series some analogues are endowed with strong antiviral properties, particularly against herpesviruses. The six-membered anhydrohexitol ring was, therefore, combined with the triazolyl carboxamide moiety of ribavirin, thus providing a new series of ribavirin analogues. None of the newly synthesized compounds elicited any substantial antiviral activity, neither against herpes simplex virus type 1 and 2, nor against bovine viral diarrhoea virus (a surrogate for hepatitis C virus) or the yellow fever virus.

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Markine-Goriaynoff, N., J. P. Georgin, M. Goltz та ін. "The Core 2 β-1,6-N-Acetylglucosaminyltransferase-Mucin Encoded by Bovine Herpesvirus 4 Was Acquired from an Ancestor of the African Buffalo". Journal of Virology 77, № 3 (2003): 1784–92. http://dx.doi.org/10.1128/jvi.77.3.1784-1792.2003.

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ABSTRACT The Bo17 gene of bovine herpesvirus 4 (BoHV-4) is the only viral gene known to date that encodes a homologue of the cellular core 2 β-1,6-N-acetylglucosaminyltransferase-mucin type (C2GnT-M). To investigate the origin and evolution of the Bo17 gene, we analyzed its distribution among BoHV-4 strains and determined the sequences of Bo17 from nine representative strains and of the C2GnT-M gene from six species of ruminants expected to encompass the group within which the gene acquisition occurred. Of 34 strains of BoHV-4, isolated from four different continents, all were found to contain the Bo17 gene. Phylogenetic analyses indicated that Bo17 was acquired from a recent ancestor of the African buffalo, implying that cattle subsequently acquired BoHV-4 by cross-species transmission. The rate of synonymous nucleotide substitution in Bo17 was estimated at 5 × 10−8 to 6 × 10−8 substitutions/site/year, consistent with previous estimates made under the assumption that herpesviruses have cospeciated with their hosts. The Bo17 gene acquisition was dated to around 1.5 million years ago. Bo17 sequences from BoHV-4 strains from African buffalo and from cattle formed two separate clades, estimated to have split about 700,000 years ago. Analysis of the ratio of nonsynonymous to synonymous nucleotide substitutions revealed a burst of amino acid replacements subsequent to the transfer of the cellular gene to the viral genome, followed by a return to a strong constraint on nonsynonymous changes during the divergence of contemporary BoHV-4 strains. The Bo17 gene represents the most recent of the known herpesvirus gene acquisitions and provides the best opportunity for learning more about this important process of viral evolution.

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Riaz, Aayesha, Inga Dry, Claire S. Levy, et al. "Ovine herpesvirus-2-encoded microRNAs target virus genes involved in virus latency." Journal of General Virology 95, no. 2 (2014): 472–80. http://dx.doi.org/10.1099/vir.0.059303-0.

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Herpesviruses encode microRNAs (miRNAs) that target both virus and host genes; however, their role in herpesvirus biology is understood poorly. We identified previously eight miRNAs encoded by ovine herpesvirus-2 (OvHV-2), the causative agent of malignant catarrhal fever (MCF), and have now investigated the role of these miRNAs in regulating expression of OvHV-2 genes that play important roles in virus biology. ORF20 (cell cycle inhibition), ORF50 (reactivation) and ORF73 (latency maintenance) each contain predicted targets for several OvHV-2 miRNAs. Co-transfection of miRNA mimics with luciferase reporter constructs containing the predicted targets showed the 5′ UTRs of ORF20 and ORF73 contain functional targets for ovhv-miR-2 and ovhv2-miR-8, respectively, and the 3′ UTR of ORF50 contains a functional target for ovhv2-miR-5. Transfection of BJ1035 cells (an OvHV-2-infected bovine T-cell line) with the relevant miRNA mimic resulted in a significant decrease in ORF50 and a smaller but non-significant decrease in ORF20. However, we were unable to demonstrate a decrease in ORF73. MCF is a disease of dysregulated lymphocyte proliferation; miRNA inhibition of ORF20 expression may play a role in this aberrant lymphocyte proliferation. The proteins encoded by ORF50 and ORF73 play opposing roles in latency. It has been hypothesized that miRNA-induced inhibition of virus genes acts to ensure that fluctuations in virus mRNA levels do not result in reactivation under conditions that are unfavourable for viral replication and our data supported this hypothesis.

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Okazaki, Katsunori. "Proteolytic cleavage of glycoprotein B is dispensable for in vitro replication, but required for syncytium formation of pseudorabies virus." Journal of General Virology 88, no. 7 (2007): 1859–65. http://dx.doi.org/10.1099/vir.0.82610-0.

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Glycoprotein B (gB) is the most conserved glycoprotein among herpesviruses and it plays important roles in virus infectivity. In most herpesviruses, including pseudorabies virus (PRV), gB is cleaved by a cellular protease into two disulfide-linked subunits. In the present study, I found that the PRV gB generated in human colon carcinoma LoVo cells, which lack the ubiquitous protease furin, remained in the uncleaved form and the virus replicated in these cells without cell fusion. The uncleaved gB was converted into its subunits after furin digestion. The virus also replicated in Madin–Darby bovine kidney cells without cell fusion in the presence of a furin inhibitor, whereas distinct syncytia were formed in the absence of the inhibitor. LoVo cells constitutively expressing furin showed cell fusion when they were infected with the virus. Penetration kinetics assays revealed that the virus carrying uncleaved gB penetrated the cells at the same rate as the virus carrying cleaved gB. These results indicate that PRV gB is cleaved by furin and that the cleavage is dispensable for virus replication in vitro. Furthermore, gB cleavage is involved in syncytium formation but not in penetration kinetics, suggesting that different mechanisms operate between cell–cell fusion and virus–cell fusion by PRV.

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D’Arce, R. C. F., R. S. Almeida, T. C. Silva, et al. "Restriction endonuclease and monoclonal antibody analysis of Brazilian isolates of bovine herpesviruses types 1 and 5." Veterinary Microbiology 88, no. 4 (2002): 315–24. http://dx.doi.org/10.1016/s0378-1135(02)00126-8.

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Bellows, David S., B. Nelson Chau, Percy Lee, Yuri Lazebnik, William H. Burns, and J. Marie Hardwick. "Antiapoptotic Herpesvirus Bcl-2 Homologs Escape Caspase-Mediated Conversion to Proapoptotic Proteins." Journal of Virology 74, no. 11 (2000): 5024–31. http://dx.doi.org/10.1128/jvi.74.11.5024-5031.2000.

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ABSTRACT The antiapoptotic Bcl-2 and Bcl-xL proteins of mammals are converted into potent proapoptotic factors when they are cleaved by caspases, a family of apoptosis-inducing proteases (E. H.-Y. Cheng, D. G. Kirsch, R. J. Clem, R. Ravi, M. B. Kastan, A. Bedi, K. Ueno, and J. M. Hardwick, Science 278:1966–1968, 1997; R. J. Clem, E. H.-Y. Cheng, C. L. Karp, D. G. Kirsch, K. Ueno, A. Takahashi, M. B. Kastan, D. E. Griffin, W. C. Earnshaw, M. A. Veliuona, and J. M. Hardwick, Proc. Natl. Acad. Sci. USA 95:554–559, 1998). Gamma herpesviruses also encode homologs of the Bcl-2 family. All tested herpesvirus Bcl-2 homologs possess antiapoptotic activity, including the more distantly related homologs encoded by murine gammaherpesvirus 68 (γHV68) and bovine herpesvirus 4 (BHV4), as described here. To determine if viral Bcl-2 proteins can be converted into death factors, similar to their cellular counterparts, five herpesvirus Bcl-2 homologs from five different viruses were tested for their susceptibility to caspases. Only the viral Bcl-2 protein encoded by γHV68 was susceptible to caspase digestion. However, unlike the caspase cleavage products of cellular Bcl-2, Bcl-xL, and Bid, which are potent inducers of apoptosis, the cleavage product of γHV68 Bcl-2 lacked proapoptotic activity. KSBcl-2, encoded by the Kaposi's sarcoma-associated herpesvirus, was the only viral Bcl-2 homolog that was capable of killing cells when expressed as an N-terminal truncation. However, because KSBcl-2 was not cleavable by caspases, the latent proapoptotic activity of KSBcl-2 apparently cannot be released. The Bcl-2 homologs encoded by herpesvirus saimiri, Epstein-Barr virus, and BHV4 were not cleaved by apoptotic cell extracts and did not possess latent proapoptotic activities. Thus, herpesvirus Bcl-2 homologs escape negative regulation by retaining their antiapoptotic activities and/or failing to be converted into proapoptotic proteins by caspases during programmed cell death.

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Bruggemann, Rafaela, Janaina Matsuo Orlandi, Fabricio Jose Benati, et al. "Antiviral activity of Agaricus blazei Murrill ss. Heinem extract against human and bovine herpesviruses in cell culture." Brazilian Journal of Microbiology 37, no. 4 (2006): 561–65. http://dx.doi.org/10.1590/s1517-83822006000400029.

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Parker, R. F., G. L. Foley, C. A. Dangler, W. Kaboord, and R. Samberg. "Necrotic Oophoritis in Gilts Associated with Experimental Inoculation of a Viral Gene-deletion Mutant Pseudorabies Vaccine." Veterinary Pathology 34, no. 3 (1997): 199–203. http://dx.doi.org/10.1177/030098589703400304.

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Previous work on the reproductive effects of various herpesviruses has demonstrated adverse effects on reproductive function in several host species. Although herpesviral vaccines are used in several species to ameliorate the clinical effects of infection, pathogenicity for reproductive tissue, associated with diminished reproductive efficiency, has been reported to be retained in a live-attenuated vaccine strain of the herpesvirus, bovine herpesvirus-1. The objective of this study was to determine if a gene-deletion mutant, thymidine kinase negative, pseudorabies virus retained acute pathogenicity for the reproductive tract of swine following intravenous inoculation during estrus. Estrous cycles of nulliparous gilts were synchronized by administration of a gonadotropin and daily exposure to a boar. During estrus, six gilts were inoculated intravenously with twice the recommended intramuscular dose of a commercially available viral gene-deletion mutant pseudorabies vaccine. Six control gilts in estrus were sham inoculated intravenously with vaccine diluent during estrus. All animals were euthanatized 10 days postinoculation, and the ovaries and uterus were collected for histopathology following gross examination. All reproductive tracts were grossly normal. Histologically, four of six treated gilts had a mild to moderate, multifocal, necrotizing oophoritis, with the lesions limited to corpora lutea and the adjacent stroma. Ovaries of control gilts exhibited no necrotizing lesions. Both control and pseudorabies vaccine-inoculated gilts had occasional minimal focal mononuclear infiltrates in the ovaries. These data show that live attenuated viral gene-deletion mutant pseudorabies vaccine administered to swine during estrus can result in acute pathogenicity in ovarian corpora lutea. No endocrinologic data is available in these pigs, so the impact on pregnancy maintenance is unknown.

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Lazic, Sava, Tamas Petrovic, Ivan Pusic, and Maja Velhner. "Most frequent calf diseases in industrial breeding." Veterinarski glasnik 58, no. 1-2 (2004): 67–76. http://dx.doi.org/10.2298/vetgl0402067l.

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It is possible to conduct an analysis of the incidence of viral diseases in calves if these diseases are divided into two basic groups. One group comprises diseases of respiratory organs which are manifested by symptoms of a respiratory syndrome, and the second group comprises diseases of digestive tract organs in the form of a gastrointestinal syndrome. It is considered that viruses have the dominant role in the complex etiology of the respiratory syndrome, primarily the IBR virus or the Bovine Herpes Virus-1 (BHV-1), followed by the parainfluenza 3 virus (RSV), the Bovine Viral Diahrrea Virus (BVDV), the bovine Respiratory Syncytial Virus (RSV), but also other viruses, such as adenoviruses, rhinoviruses, coronaviruses, can also influence the appearance of the respiratory syndrome. The respiratory syndrome is rarely caused by a single viral agent, but most frequently by mixed viruses, but also by bacterial infections. Mixed viral infections often have a lethal outcome. Investigations of the etiology of the gastrointestinal syndrome so far indicate that, in addition to bacteria, viruses can also be a significant etiological factor. Rotaviruses, coronaviruses, adenoviruses parvoviruses, herpesviruses (the IBR virus), pestiviruses (BVDV), can be the causes of a gastrointestinal syndrome. It is believed that viruses can be the cause in about 10% cases in the ethiopathogenesis of this syndrome. The paper describes the etiopathogenesis of calf diseases of viral etiology which are most often found in the local conditions of industrial breeding of calves.

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Traesel, C. K., L. M. Bernardes, F. R. Spilki, R. Weiblen, and E. F. Flores. "Sequence analysis of the 5′ third of glycoprotein C gene of South American bovine herpesviruses 1 and 5." Brazilian Journal of Medical and Biological Research 48, no. 5 (2015): 470–78. http://dx.doi.org/10.1590/1414-431x20144266.

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Del Médico Zajac, Maria Paula, Sonia Alejandra Romera, Maria Fatima Ladelfa, et al. "Characterization of interspecific recombinants generated from closely related bovine herpesviruses 1 and 5 through multiple PCR sequencing assays." Journal of Virological Methods 161, no. 1 (2009): 75–83. http://dx.doi.org/10.1016/j.jviromet.2009.05.020.

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Wang, G. H., J. Bertin, Y. Wang, et al. "Bovine herpesvirus 4 BORFE2 protein inhibits Fas- and tumor necrosis factor receptor 1-induced apoptosis and contains death effector domains shared with other gamma-2 herpesviruses." Journal of virology 71, no. 11 (1997): 8928–32. http://dx.doi.org/10.1128/jvi.71.11.8928-8932.1997.

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Journal articles on the topic 'Bovine herpesviruses'

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