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Journal articles on the topic 'Cell sheet technology'

Author: Grafiati

Published: 7 June 2025

Last updated: 17 July 2025

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1

Jiang, Zhiwei, Yue Xi, Kaichen Lai, Ying Wang, Huiming Wang, and Guoli Yang. "Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology." BioMed Research International 2017 (2017): 1–11. http://dx.doi.org/10.1155/2017/9474573.

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Rat bone marrow mesenchymal stem cell sheets (rBMSC sheets) are attractive for cell-based tissue engineering. However, methods of culturing rBMSC sheets are critically limited. In order to obtain intact rBMSC sheets, a light-induced cell sheet method was used in this study. TiO2 nanodot films were coated with (TL) or without (TN) laminin-521. We investigated the effects of laminin-521 on rBMSCs during cell sheet culturing. The fabricated rBMSC sheets were subsequently assessed to study cell sheet viability, reattachment ability, cell sheet thickness, collagen type I deposition, and multilineage potential. The results showed that laminin-521 could promote the formation of rBMSC sheets with good viability under hyperconfluent conditions. Cell sheet thickness increased from an initial 26.7 ± 1.5 μm (day 5) up to 47.7 ± 3.0 μm (day 10). Moreover, rBMSC sheets maintained their potential of osteogenic, adipogenic, and chondrogenic differentiation. This study provides a new strategy to obtain rBMSC sheets using light-induced cell sheet technology.

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2

Sakaguchi, Katsuhisa, Kei Akimoto, Masanori Takaira, Ryu-ichiro Tanaka, Tatsuya Shimizu, and Shinjiro Umezu. "Cell-Based Microfluidic Device Utilizing Cell Sheet Technology." Cyborg and Bionic Systems 2022 (January 27, 2022): 1–8. http://dx.doi.org/10.34133/2022/9758187.

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The development of microelectromechanical systems has resulted in the rapid development of polydimethylpolysiloxane (PDMS) microfluidic devices for drug screening models. Various cell functions, such as the response of endothelial cells to fluids, have been elucidated using microfluidic devices. Additionally, organ-on-a-chip systems that include organs that are important for biological circulation, such as the heart, liver, pancreas, kidneys, and brain, have been developed. These organs realize the biological circulation system in a manner that cannot be reproduced by artificial organs; however, the flow channels between the organs are often artificially created by PDMS. In this study, we developed a microfluidic device consisting only of cells, by combining cell sheet technology with microtitanium wires. Microwires were placed between stacked fibroblast cell sheets, and the cell sheets adhered to each other, after which the microwires were removed leaving a luminal structure with a size approximately equal to the arteriolar size. The lumen structure was constructed using wires with diameters of 50, 100, 150, and 200 μm, which were approximations of the arteriole diameters. Furthermore, using a perfusion device, we successfully perfused the luminal structure created inside the cell sheets. The results revealed that a culture solution can be supplied to a cell sheet with a very high cell density. The biofabrication technology proposed in this study can contribute to the development of organ-on-a-chip systems.

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3

Zhou, Ying, Lingqing Dong, Chao Liu, et al. "Engineering prevascularized composite cell sheet by light-induced cell sheet technology." RSC Advances 7, no. 52 (2017): 32468–77. http://dx.doi.org/10.1039/c7ra05333a.

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4

Koo, Min-Ah, Mi Hee Lee, and Jong-Chul Park. "Recent Advances in ROS-Responsive Cell Sheet Techniques for Tissue Engineering." International Journal of Molecular Sciences 20, no. 22 (2019): 5656. http://dx.doi.org/10.3390/ijms20225656.

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Cell sheet engineering has evolved rapidly in recent years as a new approach for cell-based therapy. Cell sheet harvest technology is important for producing viable, transplantable cell sheets and applying them to tissue engineering. To date, most cell sheet studies use thermo-responsive systems to detach cell sheets. However, other approaches have been reported. This review provides the progress in cell sheet detachment techniques, particularly reactive oxygen species (ROS)-responsive strategies. Therefore, we present a comprehensive introduction to ROS, their application in regenerative medicine, and considerations on how to use ROS in cell detachment. The review also discusses current limitations and challenges for clarifying the mechanism of the ROS-responsive cell sheet detachment.

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5

Akiyama, Y. "Design of Temperature-Responsive Cell Culture Surfaces for Cell Sheet Engineering." Cyborg and Bionic Systems 2021 (February 3, 2021): 1–15. http://dx.doi.org/10.34133/2021/5738457.

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Temperature-responsive cell culture surfaces, which modulate cell attachment/detachment characteristics with temperature, have been used to fabricate cell sheets. Extensive study on fabrication of cell sheet with the temperature-responsive cell culture surface, manipulation, and transplantation of the cell sheet has established the interdisciplinary field of cell sheet engineering, in which engineering, biological, and medical fields closely collaborate. Such collaboration has pioneered cell sheet engineering, making it a promising and attractive technology in tissue engineering and regenerative medicine. This review introduces concepts of cell sheet engineering, followed by designs for the fabrication of various types of temperature-responsive cell culture surfaces and technologies for cell sheet manipulation. The development of various methods for the fabrication of temperature-responsive cell culture surfaces was also summarized. The availability of cell sheet engineering for the treatment and regeneration of damaged human tissue has also been described, providing examples of the clinical application of cell sheet transplantation in humans.

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6

Imashiro, Chikahiro, and Tatsuya Shimizu. "Fundamental Technologies and Recent Advances of Cell-Sheet-Based Tissue Engineering." International Journal of Molecular Sciences 22, no. 1 (2021): 425. http://dx.doi.org/10.3390/ijms22010425.

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Tissue engineering has attracted significant attention since the 1980s, and the applications of tissue engineering have been expanding. To produce a cell-dense tissue, cell sheet technology has been studied as a promising strategy. Fundamental techniques involving tissue engineering are mainly introduced in this review. First, the technologies to fabricate a cell sheet were reviewed. Although temperature-responsive polymer-based technique was a trigger to establish and spread cell sheet technology, other methodologies for cell sheet fabrication have also been reported. Second, the methods to improve the function of the cell sheet were investigated. Adding electrical and mechanical stimulation on muscle-type cells, building 3D structures, and co-culturing with other cell species can be possible strategies for imitating the physiological situation under in vitro conditions, resulting in improved functions. Finally, culture methods to promote vasculogenesis in the layered cell sheets were introduced with in vivo, ex vivo, and in vitro bioreactors. We believe the present review that shows and compares the fundamental technologies and recent advances for cell-sheet-based tissue engineering should promote further development of tissue engineering. The development of cell sheet technology should promote many bioengineering applications.

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7

Sawa, Yoshiki, and Shigeru Miyagawa. "Cell Sheet Technology for Heart Failure." Current Pharmaceutical Biotechnology 14, no. 1 (2013): 61–66. http://dx.doi.org/10.2174/1389201011314010009.

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8

Sawa, Yoshiki, and Shigeru Miyagawa. "Cell Sheet Technology for Heart Failure." Current Pharmaceutical Biotechnology 14, no. 1 (2013): 61–66. http://dx.doi.org/10.2174/138920113804805395.

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9

Hannachi, Imen Elloumi, Masayuki Yamato, and Teruo Okano. "Cell sheet technology and cell patterning for biofabrication." Biofabrication 1, no. 2 (2009): 022002. http://dx.doi.org/10.1088/1758-5082/1/2/022002.

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10

Hong, Yi, Mengfei Yu, Wenjian Weng, Kui Cheng, Huiming Wang, and Jun Lin. "Light-induced cell detachment for cell sheet technology." Biomaterials 34, no. 1 (2013): 11–18. http://dx.doi.org/10.1016/j.biomaterials.2012.09.043.

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11

Yorukoglu, A. Cagdas, A. Esat Kiter, Semih Akkaya, N. Lale Satiroglu-Tufan, and A. Cevik Tufan. "A Concise Review on the Use of Mesenchymal Stem Cells in Cell Sheet-Based Tissue Engineering with Special Emphasis on Bone Tissue Regeneration." Stem Cells International 2017 (2017): 1–13. http://dx.doi.org/10.1155/2017/2374161.

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The integration of stem cell technology and cell sheet engineering improved the potential use of cell sheet products in regenerative medicine. This review will discuss the use of mesenchymal stem cells (MSCs) in cell sheet-based tissue engineering. Besides their adhesiveness to plastic surfaces and their extensive differentiation potential in vitro, MSCs are easily accessible, expandable in vitro with acceptable genomic stability, and few ethical issues. With all these advantages, they are extremely well suited for cell sheet-based tissue engineering. This review will focus on the use of MSC sheets in osteogenic tissue engineering. Potential application techniques with or without scaffolds and/or grafts will be discussed. Finally, the importance of osteogenic induction of these MSC sheets in orthopaedic applications will be demonstrated.

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12

Bou-Ghannam, Sophia, Kyungsook Kim, Makoto Kondo, David W. Grainger, and Teruo Okano. "Mesenchymal Stem Cell Sheet Centrifuge-Assisted Layering Augments Pro-Regenerative Cytokine Production." Cells 11, no. 18 (2022): 2840. http://dx.doi.org/10.3390/cells11182840.

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A focal advantage of cell sheet technology has been as a scaffold-free three-dimensional (3D) cell delivery platform capable of sustained cell engraftment, survival, and reparative function. Recent evidence demonstrates that the intrinsic cell sheet 3D tissue-like microenvironment stimulates mesenchymal stem cell (MSC) paracrine factor production. In this capacity, cell sheets not only function as 3D cell delivery platforms, but also prime MSC therapeutic paracrine capacity. This study introduces a “cell sheet multilayering by centrifugation” strategy to non-invasively augment MSC paracrine factor production. Cell sheets fabricated by temperature-mediated harvest were first centrifuged as single layers using optimized conditions of rotational speed and time. Centrifugation enhanced cell physical and biochemical interactions related to intercellular communication and matrix interactions within the single cell sheet, upregulating MSC gene expression of connexin 43, integrin β1, and laminin α5. Single cell sheet centrifugation triggered MSC functional enhancement, secreting higher concentrations of pro-regenerative cytokines vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and interleukin-10 (IL-10). Subsequent cell sheet stacking, and centrifugation generated cohesive, bilayer MSC sheets within 2 h, which could not be accomplished within 24 h by conventional layering methods. Conventional layering led to H1F-1α upregulation and increased cell death, indicating a hypoxic thickness limitation to this approach. Comparing centrifuged single and bilayer cell sheets revealed that layering increased VEGF production 10-fold, attributed to intercellular interactions at the layered sheet interface. The “MSC sheet multilayering by centrifugation” strategy described herein generates a 3D MSC-delivery platform with boosted therapeutic factor production capacity.

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13

Lin, Gwo-Long, and Peng-Hsin Chen. "Dissemination of Biomedical Cell Sheets using Reality Technology and Digital Tools." Journal of Humanities and Social Sciences Studies 3, no. 4 (2021): 46–53. http://dx.doi.org/10.32996/jhsss.2021.3.4.5.

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Nowadays, with the advance of biomedical and pharmaceutical technology, new treatments such as cell therapy, cell sheets, etc. all provide patients other alternatives. However, the public has little knowledge in these new technologies and they also find them difficult to understand. Hence, this paper is aimed at providing the public with state-of-the-art technological knowledge and constructing an innovative field full of diversity, entertainment, and educational meaning with the assistance of Augmented Reality (AR) and Virtual Reality (VR). Taking the cell sheets technology, a kind of cell therapy recently approved in Taiwan, as an example, we built an AR platform, and demonstrated a trailer animation, 2D animation and 3D model animation via Merge Cube. The trailer animation will portrait how the main character helps her friend, who became physically challenged in an accident, stand up again by asking a genius doctor to perform cell sheet technology. The 2D animation will be used to explain how cell sheet works and its application, while the 3D animation helps demonstrate the DNA reproduction and cell division in cell therapy. A VR field will also be set up so that players can play as the genius doctor, fight their way through the VR games, and learn more about cell sheet technology. To let the public learn more about this biotechnology knowledge, we held an exhibition to display the research results, providing them a whole new learning experience.

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14

Lee, Yu Na, Hye-Jin Yi, Yang Hee Kim, et al. "Evaluation of Multi-Layered Pancreatic Islets and Adipose-Derived Stem Cell Sheets Transplanted on Various Sites for Diabetes Treatment." Cells 9, no. 9 (2020): 1999. http://dx.doi.org/10.3390/cells9091999.

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Islet cell transplantation is considered an ideal treatment for insulin-deficient diabetes, but implantation sites are limited and show low graft survival. Cell sheet technology and adipose-derived stem cells (ADSCs) can be useful tools for improving islet cell transplantation outcomes since both can increase implantation efficacy and graft survival. Herein, the optimal transplantation site in diabetic mice was investigated using islets and stem cell sheets. We constructed multi-layered cell sheets using rat/human islets and human ADSCs. Cell sheets were fabricated using temperature-responsive culture dishes. Islet/ADSC sheet (AI sheet) group showed higher viability and glucose-stimulated insulin secretion than islet-only group. Compared to islet transplantation alone, subcutaneous AI sheet transplantation showed better blood glucose control and CD31+ vascular traits. Because of the adhesive properties of cell sheets, AI sheets were easily applied on liver and peritoneal surfaces. Liver or peritoneal surface grafts showed better glucose control, weight gain, and intraperitoneal glucose tolerance test (IPGTT) profiles than subcutaneous site grafts using both rat and human islets. Stem cell sheets increased the therapeutic efficacy of islets in vivo because mesenchymal stem cells enhance islet function and induce neovascularization around transplanted islets. The liver and peritoneal surface can be used more effectively than the subcutaneous site in future clinical applications.

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15

Tatsumi, Kohei, and Teruo Okano. "Hepatocyte Transplantation: Cell Sheet Technology for Liver Cell Transplantation." Current Transplantation Reports 4, no. 3 (2017): 184–92. http://dx.doi.org/10.1007/s40472-017-0156-7.

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16

Dergilev, K., Z. Tsokolaeva, P. Makarevich, et al. "C-Kit Cardiac Progenitor Cell Based Cell Sheet Improves Vascularization and Attenuates Cardiac Remodeling following Myocardial Infarction in Rats." BioMed Research International 2018 (June 25, 2018): 1–13. http://dx.doi.org/10.1155/2018/3536854.

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The adult heart contains small populations of multipotent cardiac progenitor cells (CPC) that present a convenient and efficient resource for treatment of myocardial infarction. Several clinical studies of direct CPC delivery by injection have already been performed but showed low engraftment rate that limited beneficial effects of procedure. «Cell sheet» technology has been developed to facilitate longer retention of grafted cells and show new directions for cell-based therapy using this strategy. In this study we hypothesized that СPC-based cell sheet transplantation could improve regeneration after myocardial infarction. We demonstrated that c-kit+ CPC were able to form cell sheets on temperature-responsive surfaces. Cell sheet represented a well-organized structure, in which CPC survived, retained ability to proliferate, expressed progenitor cell marker Gata-4 formed connexin-43+ gap junctions, and were surrounded by significant amount of extracellular matrix proteins. Transplantation of cell sheets after myocardial infarction resulted in CPC engraftment as well as their proliferation, migration, and differentiation; cell sheets also stimulated neovascularization and cardiomyocyte proliferation in underlining myocardium and ameliorated left ventricular remodeling. Obtained data strongly supported potential use of CPC sheet transplantation for repair of damaged heart.

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17

Ohki, Takeshi, and Masakazu Yamamoto. "Esophageal regenerative therapy using cell sheet technology." Regenerative Therapy 13 (March 2020): 8–17. http://dx.doi.org/10.1016/j.reth.2020.04.009.

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18

Collins, A. J., K. Narwani, C. C. Calhoun, et al. "Cell Sheet Technology for Corneal Surface Reconstruction." Journal of Oral and Maxillofacial Surgery 78, no. 10 (2020): e85. http://dx.doi.org/10.1016/j.joms.2020.07.168.

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19

Matsuda, N., T. Shimizu, M. Yamato, and T. Okano. "Tissue Engineering Based on Cell Sheet Technology." Advanced Materials 19, no. 20 (2007): 3089–99. http://dx.doi.org/10.1002/adma.200701978.

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20

Sato, Masato, Masayuki Yamato, Kosuke Hamahashi, Teruo Okano, and Joji Mochida. "Articular Cartilage Regeneration Using Cell Sheet Technology." Anatomical Record 297, no. 1 (2013): 36–43. http://dx.doi.org/10.1002/ar.22829.

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21

Kawamura, Takuji. "Cardiac Regeneration Therapy Using Cell Sheet Technology." Drug Delivery System 40, no. 2 (2025): 127–31. https://doi.org/10.2745/dds.40.127.

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22

Dergilev, K. V., P. I. Makarevich, M. Yu Menshikov, and E. V. Parfyonova. "Application of tissue engineered constructs on the basis of cell sheets FOR RESTORATION OF TISSUES AND ORGANS." Genes & Cells 11, no. 3 (2016): 23–32. http://dx.doi.org/10.23868/gc120564.

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Cell sheet technology has certain advantages over conventionally used injections as far as it facilitates cell survival and integration after delivery of cells to intended organ/tissue. It also allows to successfully replace lost or irreversibly damaged tissues with restoration of its functions including endo/paracrine activity. Application of cell sheets has gone beyond bench work and now is under clinical translation where it is successfully used for repair of cornea, cartilage, periodontal tissue, esophageal mucosa, pancreas and thyroid gland. Further advances of cell sheet technologies allow to construct pre-vascularized tissue grafts which effects are not limited to tissue repair, but also allows to restore its function via paracrine action of transplanted cells and to ensure long-lasting therapeutic effects. Genetic modification of cells used for cell sheet construction allows to utilize this technology to treat hereditary disorders, deficit of enzymes or other secreted proteins. This review focuses on recent results of therapeutic implication of cell sheets and prospects of this field which gained much attention in regenerative medicine.

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23

Yang, Joseph, Masayuki Yamato, and Teruo Okano. "Cell-Sheet Engineering Using Intelligent Surfaces." MRS Bulletin 30, no. 3 (2005): 189–93. http://dx.doi.org/10.1557/mrs2005.51.

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AbstractThe possibility of recreating various tissues and organs for the purpose of regenerative medicine has received much interest. However, the field of tissue engineering has been restricted by the limitations of conventional approaches. A method to circumvent the need for traditional scaffold-based technologies is cell-sheet engineering, which uses temperature-responsive culture dishes. These surfaces, which are created by grafting the temperature-responsive polymer poly(N-isopropylacrylamide) onto ordinary culture dishes, enable the non-invasive harvesting of cells as intact sheets by simple temperature reduction. This article reviews current research on the applications of cell-sheet engineering for the reconstruction of various tissues, as well as the intelligent surfaces used by this novel technology.

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24

Kim, Hyunbum, Yunhye Kim, Jihyun Park, Nathaniel Hwang, Yun Lee, and Yongsung Hwang. "Recent Advances in Engineered Stem Cell-Derived Cell Sheets for Tissue Regeneration." Polymers 11, no. 2 (2019): 209. http://dx.doi.org/10.3390/polym11020209.

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The substantial progress made in the field of stem cell-based therapy has shown its significant potential applications for the regeneration of defective tissues and organs. Although previous studies have yielded promising results, several limitations remain and should be overcome for translating stem cell-based therapies to clinics. As a possible solution to current bottlenecks, cell sheet engineering (CSE) is an efficient scaffold-free method for harvesting intact cell sheets without the use of proteolytic enzymes, and may be able to accelerate the adoption of stem cell-based treatments for damaged tissues and organs regeneration. CSE uses a temperature-responsive polymer-immobilized surface to form unique, scaffold-free cell sheets composed of one or more cell layers maintained with important intercellular junctions, cell-secreted extracellular matrices, and other important cell surface proteins, which can be achieved by changing the surrounding temperature. These three-dimensional cell sheet-based tissues can be designed for use in clinical applications to target-specific tissue regeneration. This review will highlight the principles, progress, and clinical relevance of current approaches in the cell sheet-based technology, focusing on stem cell-based therapies for bone, periodontal, skin, and vascularized muscles.

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25

Ochiai, Jun, Yutaka Niihara, and Joan Oliva. "Measurement of the Adipose Stem Cells Cell Sheets Transmittance." Bioengineering 8, no. 7 (2021): 93. http://dx.doi.org/10.3390/bioengineering8070093.

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In the field of cell therapy, the interest in cell sheet technology is increasing. To determine the cell sheet harvesting time requires experience and practice, and different factors could change the harvesting time (variability among donors and culture media, between cell culture dishes, initial cell seeding density). We have developed a device that can measure the transmittance of the multilayer cell sheets, using a light emitting diode and a light detector, to estimate the harvesting time. The transmittance of the adipose stromal cells cell sheets (ASCCS) was measured every other day as soon as the cells were confluent, up to 12 days. The ASCCS, from three different initial seeding densities, were harvested at 8, 10, and 12 days after seeding. Real-time PCR and immunostaining confirmed the expression of specific cell markers (CD29, CD73, CD90, CD105, HLA-A, HLA-DR), but less than the isolated adipose stromal cells. The number of cells per cell sheets, the average thickness per cell sheet, and the corresponding transmittance showed no correlation. Decrease of the transmittance seems to be correlated with the cell sheet maturation. For the first time, we are reporting the success development of a device to estimate ASCCS harvesting time based on their transmittance.

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Yu, Meng-Liu, Meng-Fei Yu, Li-Qin Zhu, Tian-Tian Wang, Yi Zhou, and Hui-Ming Wang. "The Effects of TiO2Nanodot Films with RGD Immobilization on Light-Induced Cell Sheet Technology." BioMed Research International 2015 (2015): 1–10. http://dx.doi.org/10.1155/2015/582359.

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Cell sheet technology is a new strategy in tissue engineering which could be possible to implant into the body without a scaffold. In order to get an integrated cell sheet, a light-induced method via UV365 is used for cell sheet detachment from culture dishes. In this study, we investigated the possibility of cell detachment and growth efficiency on TiO2nanodot films with RGD immobilization on light-induced cell sheet technology. Mouse calvaria-derived, preosteoblastic (MC3T3-E1) cells were cultured on TiO2nanodot films with (TR) or without (TN) RGD immobilization. After cells were cultured with or without 5.5 mW/cm2UV365 illumination, cell morphology, cell viability, osteogenesis related RNA and protein expression, and cell detachment ability were compared, respectively. Light-induced cell detachment was possible when cells were cultured on TR samples. Also, cells cultured on TR samples showed better cell viability, alongside higher protein and RNA expression than on TN samples. This study provides a new biomaterial for light-induced cell/cell sheet harvesting.

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Xu, Xiaoru, Shuang Song, Xiangdong Liu, et al. "The Proangiogenic Potential of Rat Adipose-Derived Stromal Cells with and without Cell-Sheet Induction: A Comparative Study." Stem Cells International 2022 (October 5, 2022): 1–17. http://dx.doi.org/10.1155/2022/2601764.

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A functional vasculature for survival remains a challenge for tissue regeneration, which is indispensable for oxygen and nutrient supply. Utilizing mesenchymal stromal cells (MSCs) to alleviate tissue ischemia and repair dysfunctional or damaged endothelium is a promising strategy. Compared to other populations of MSCs, adipose-derived stromal cells (ASCs) possess a more significant proangiogenic potential and are abundantly available. Cell sheet technology has recently been widely utilized in bone engineering. Compared to conventional methods of seeding seed cell suspension onto biological scaffolds, cell sheet technology prevents cell loss and preserves the extracellular matrix (ECM). Nevertheless, the proangiogenic potential of ASC sheets remains unknown. In this study, rat ASC sheets were constructed, and their macro- and microstructures were examined. In addition, we investigated the effects of ASCs and ASC sheets on the biological properties and angiogenic capacity of endothelial cells (ECs). The results demonstrated that the ASC sheets gradually thickened as the number of cells and ECM increased over time and that the cells were in an active state of secretion. Similar to ASC-CM, the conditioned medium (CM) of ASC sheets could significantly enhance the proliferative capacity of ECs. ASC sheet-CM has significant advantages over ASC-CM in promoting the migration and angiogenesis of ECs, where the exosomes secreted by ASC sheets play an essential role. Therefore, using ASC sheets for therapeutic tissue and organ regeneration angiogenesis may be a valuable strategy.

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Sekiya, Sachiko, Tatsuya Shimizu, and Teruo Okano. "Vascularization in 3D tissue using cell sheet technology." Regenerative Medicine 8, no. 3 (2013): 371–77. http://dx.doi.org/10.2217/rme.13.16.

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Haraguchi, Yuji, Tatsuya Shimizu, Masayuki Yamato, and Teruo Okano. "Scaffold-free tissue engineering using cell sheet technology." RSC Advances 2, no. 6 (2012): 2184. http://dx.doi.org/10.1039/c2ra00704e.

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30

Ishii, Rie, Ubaidus Sobhan, Tatsuzo Hebiguchi, et al. "Fetal myelomeningocele repair based on cell sheet technology." Japanese Journal of SURGICAL METABOLISM and NUTRITION 48, no. 6 (2014): 215–18. http://dx.doi.org/10.11638/jssmn.48.6_215.

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31

Jiang, Zhiwei, Danji Zhu, Ke Yu, Yue Xi, Xiaozhao Wang, and Guoli Yang. "Recent advances in light-induced cell sheet technology." Acta Biomaterialia 119 (January 2021): 30–41. http://dx.doi.org/10.1016/j.actbio.2020.10.044.

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32

Liu, Chao, Ying Zhou, Miao Sun, et al. "Light-Induced Cell Alignment and Harvest for Anisotropic Cell Sheet Technology." ACS Applied Materials & Interfaces 9, no. 42 (2017): 36513–24. http://dx.doi.org/10.1021/acsami.7b07202.

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33

Komae, Hyoe, Minoru Ono, and Tatsuya Shimizu. "Cell Sheet-Based Vascularized Myocardial Tissue Fabrication." European Surgical Research 59, no. 3-4 (2018): 276–85. http://dx.doi.org/10.1159/000492416.

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Background: The development of regenerative medicine in recent years has been remarkable as tissue engineering technology and stem cell research have advanced. The ultimate goal of regenerative medicine is to fabricate human organs artificially. If fabricated organs can be transplanted medically, it will be the innovative treatment of diseases for which only donor organ transplantation is the definitive therapeutic method at present. Summary: Our group has reported successful fabrication of thick functional myocardial tissue in vivo and in vitro by using cell sheet engineering technology which requires no scaffolds. Thick myocardial tissue can be fabricated by stacking cardiomyocyte sheets on the vascular bed every 24 h, so that a vascular network can be formed within the myocardial graft. We call this procedure a multi-step transplantation procedure. After human-induced pluripotent stem cells were discovered and human cardiomyocytes became available, a thick, macroscopically pulsate human myocardial tissue was successfully constructed by using a multi-step transplantation procedure. Furthermore, our group succeeded in fabricating functional human myocardial tissue which can generate pressure. Here, we present our way of fabricating human myocardial tissue by means of cell sheet engineering technology. Key Messages: Our group succeeded in fabricating thick, functional human myocardium which can generate pulse pressure. However, there are still a few problems to be solved until clinically functional human cardiac tissue or a whole heart can be fabricated. Research on myocardial regeneration progresses at such a pace that we believe the products of this research will save many lives in the near future.

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Park, Jeong Eun, Won Seok Choi, and Donggun Lim. "Cell/Module Integration Technology with Wire-Embedded EVA Sheet." Applied Sciences 11, no. 9 (2021): 4170. http://dx.doi.org/10.3390/app11094170.

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Silicon wafers are crucial for determining the price of solar cell modules. To reduce the manufacturing cost of photovoltaic devices, the thicknesses of wafers are reduced. However, the conventional module manufacturing method using the tabbing process has a disadvantage in that the cell is damaged because of the high temperature and pressure of the soldering process, which is complicated, thus increasing the process cost. Consequently, when the wafer is thinned, the breakage rate increases during the module process, resulting in a lower yield; further, the module performance decreases owing to cracks and thermal stress. To solve this problem, a module manufacturing method is proposed in which cells and wires are bonded through the lamination process. This method minimizes the thermal damage and mechanical stress applied to solar cells during the tabbing process, thereby manufacturing high-power modules. When adopting this method, the front electrode should be customized because it requires busbarless solar cells different from the existing busbar solar cells. Accordingly, the front electrode was designed using various simulation programs such as Griddler 2.5 and MathCAD, and the effect of the diameter and number of wires in contact with the front finger line of the solar cell on the module characteristics was analyzed. Consequently, the efficiency of the module manufactured with 12 wires and a wire diameter of 0.36 mm exhibited the highest efficiency at 20.28%. This is because even if the optical loss increases with the diameter of the wire, the series resistance considerably decreases rather than the loss of the short-circuit current, thereby improving the fill factor. The characteristics of the wire-embedded ethylene vinyl acetate (EVA) sheet module were confirmed to be better than those of the five busbar tabbing modules manufactured by the tabbing process; further, a high-power module that sufficiently compensated for the disadvantages of the tabbing module was manufactured.

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35

Wang, Jing, Rui Zhang, Yun Shen, et al. "Recent Advances in Cell Sheet Technology for Periodontal Regeneration." Current Stem Cell Research & Therapy 9, no. 3 (2014): 162–73. http://dx.doi.org/10.2174/1574888x09666140213150218.

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Balsam, Leora B. "Recapitulating nature's design: Myocardial repair with cell sheet technology." Journal of Thoracic and Cardiovascular Surgery 154, no. 3 (2017): 951–52. http://dx.doi.org/10.1016/j.jtcvs.2017.05.011.

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LI, MINXIONG, JUN MA, YANBIN GAO, and LEI YANG. "Cell sheet technology: a promising strategy in regenerative medicine." Cytotherapy 21, no. 1 (2019): 3–16. http://dx.doi.org/10.1016/j.jcyt.2018.10.013.

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Sekine, Waki, Yuji Haraguchi, Tatsuya Shimizu, Masayuki Yamato, Akihiro Umezawa, and Teruo Okano. "Chondrocyte Differentiation of Human Endometrial Gland-Derived MSCs in Layered Cell Sheets." Scientific World Journal 2013 (2013): 1–7. http://dx.doi.org/10.1155/2013/359109.

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Recently, regenerative medicine using engineered three-dimensional (3D) tissues has been focused. In the fields of cell therapy and regenerative medicine, mesenchymal stem cells (MSCs) are attractive autologous cell sources. While, in bioengineered tissues, a 3D environment may affect the differentiation of the stem cells, little is known regarding the effect of 3D environment on cellular differentiation. In this study, MSC differentiation inin vitro3D tissue models was assessed by human endometrial gland-derived MSCs (hEMSCs) and cell sheet technology. hEMSC sheets were layered into cell-dense 3D tissues and were cultured on porous membranes. The tissue sections revealed that chondrocyte-like cells were found within the multilayered cell sheets even at 24 h after layering. Immunostainings of chondrospecific markers were positive within those cell sheet constructs. In addition, sulfated glycosaminoglycan accumulation within the tissues increased in proportion to the numbers of layered cell sheets. The findings suggested that a high cell density and hypoxic environment in 3D tissues by layering cell sheets might accelerate a rapid differentiation of hEMSCs into chondrocytes without the help of chondro-differentiation reagents. These tissue models using cell sheets would give new insights to stem cell differentiation in 3D environment and contribute to the future application of stem cells to cartilage regenerative therapy.

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Sekiya, Sachiko, Shunichi Morikawa, Taichi Ezaki, and Tatsuya Shimizu. "Pathological Process of Prompt Connection between Host and Donor Tissue Vasculature Causing Rapid Perfusion of the Engineered Donor Tissue after Transplantation." International Journal of Molecular Sciences 19, no. 12 (2018): 4102. http://dx.doi.org/10.3390/ijms19124102.

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The shortage of donors for transplantation therapy is a serious issue worldwide. Tissue engineering is considered a potential solution to this problem. Connection and perfusion in engineered tissues after transplantation is vital for the survival of the transplanted tissue, especially for tissues requiring blood perfusion to receive nutrients, such as the heart. A myocardial cell sheet containing an endothelial cell network structure was fabricated in vitro using cell sheet technology. Transplantation of the three-dimensional (3D) tissue by layering myocardial sheets could ameliorate ischemic heart disease in a rat model. The endothelial cell network in the 3D tissue was able to rapidly connect to host vasculature and begin perfusion within 24 h after transplantation. In this review, we compare and discuss the engineered tissue–host vasculature connection process between tissue engineered constructs with hydrogels and cell sheets by histological analysis. This review provides information that may be useful for further improvements of in vivo engineered tissue vascularization techniques.

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Astuty, Syanti Wahyu, Hari Sunarto, Lisa Amir, and Erik Idrus. "EVALUATION OF REGENERATIVE THERAPY USING CELL SHEET THROUGH CEMENTUM PROTEIN-1 EXPRESSION ON MACACA NEMESTRINA." International Journal of Applied Pharmaceutics 9 (January 1, 2018): 107. http://dx.doi.org/10.22159/ijap.2017.v9s2.26.

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Objective: The main objective of periodontal therapy is tissue regeneration. Previous studies have identified the potential of mesenchymal stem cells to improve major periodontal defect reconstruction in bone tissue engineering. Cell sheet technology (CST), in which a cell culture is obtained from a material coated with a temperature-sensitive substrate, has been developed for the reconstruction of various tissues, including periodontal tissue. Cementum protein-1 (CEMP-1) is a 50-kDa protein that plays a crucial role in cementogenesis by enhancing the combining of cells formed by cell cementoblast. Evaluate periodontal tissue regeneration with CST, and the application of chitosan, chitosan cell sheet or arginineglycineaspartic acid (RGD)-modified chitosan cell sheet on Macaca nemestrina one-wall defect model.Methods: The CEMP-1 was analyzed expression in a sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel electrophoresis assay.Results: CEMP-1 expression in gingival crevicular fluid was observed using the SDS-PAGE method every week for 3 weeks. Protein band expressions on SDS-PAGE gel were identified at around 50 kDa with different thicknesses between groups. The chitosan, chitosan cell sheet, and RGD-modified chitosan cell sheet groups showed protein bands of CEMP-1 between 50 and 70 kDa at weeks 1, 2, and 3; weeks 2 and 3; and weeks 1 and 2, respectively.Conclusion: Our results demonstrated that the application of chitosan and RGD-modified chitosan cell sheets could enhance bone regeneration, as evidenced by CEMP-1 protein expression levels.

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Rahmi, Gabriel, Laetitia Pidial, Amanda K. A. Silva, et al. "Designing 3D Mesenchymal Stem Cell Sheets Merging Magnetic and Fluorescent Features: When Cell Sheet Technology Meets Image-Guided Cell Therapy." Theranostics 6, no. 5 (2016): 739–51. http://dx.doi.org/10.7150/thno.14064.

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Ohki, Takeshi, Masayuki Yamamoto, Masaho Ota, Teruo Okano, and Masakazu Yamamoto. "Application of cell sheet technology for esophageal endoscopic submucosal dissection." Techniques in Gastrointestinal Endoscopy 13, no. 1 (2011): 105–9. http://dx.doi.org/10.1016/j.tgie.2011.01.003.

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Masuda, Shinako, and Tatsuya Shimizu. "Three-dimensional cardiac tissue fabrication based on cell sheet technology." Advanced Drug Delivery Reviews 96 (January 2016): 103–9. http://dx.doi.org/10.1016/j.addr.2015.05.002.

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Haraguchi, Yuji, Tatsuya Shimizu, Masayuki Yamato, and Teruo Okano. "ChemInform Abstract: Scaffold-Free Tissue Engineering Using Cell Sheet Technology." ChemInform 43, no. 21 (2012): no. http://dx.doi.org/10.1002/chin.201221272.

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Iwaya, Keiichi, Hisae Arai, Nanao Takatou, et al. "A sheet pocket to prevent cross-contamination of formalin-fixed paraffin-embedded block for application in next generation sequencing." PLOS ONE 17, no. 5 (2022): e0266947. http://dx.doi.org/10.1371/journal.pone.0266947.

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Formalin-fixed paraffin-embedded (FFPE) blocks are used as biomaterials for next-generation sequencing of cancer panels. Cross-contamination is detected in approximately 5% of the DNA extracted from FFPE samples, which reduces the detection rate of genetic abnormalities. There are no effective methods available for processing FFPE blocks that prevent cells from mixing with other specimens. The present study evaluated 897 sheets that could potentially prevent cell transmission but allow for the movement of various solvents used in FFPE blocks. According to the International Organization for Standardization and Japanese Industrial Standards, six requirements were established for the screening of packing sheets: 1) filter opening ≤5 μm, 2) thickness ≤100 μm, 3) chemical resistance, 4) permeability ≥1.0 × 10−3 cm/s, 5) water retention rate <200%, and 6) cell transit test (≤2 cells/10 high-power fields). Polyamide, polyethylene terephthalate, and polypropylene/polyethylene composite sheets met all criteria. A pocket, which was designed to wrap the tissue uniformly, was made of these sheets and was found to effectively block the entry of all cell types during FFPE block processing. Using a sheet pocket, no single cell from the cell pellet could pass through the outer layer. The presence or absence of the sheet pocket did not affect hematoxylin and eosin staining. When processing FFPE blocks as a biomaterial for next-generation sequencing, the sheet pocket was effective in preventing cross-contamination. This technology will in part support the precise translation of histopathological data into genome sequencing data in general pathology laboratories.

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Wang, Bin, Kyungsook Kim, Mi Tian, et al. "Engineered Bone Marrow Stem Cell-Sheets Alleviate Renal Damage in a Rat Chronic Glomerulonephritis Model." International Journal of Molecular Sciences 24, no. 4 (2023): 3711. http://dx.doi.org/10.3390/ijms24043711.

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Although mesenchymal stem cell (MSC)-based regenerative therapy is being developed for the treatment of kidney diseases, cell delivery and engraftment still need to be improved. Cell sheet technology has been developed as a new cell delivery method, to recover cells as a sheet form retaining intrinsic cell adhesion proteins, which promotes its transplantation efficiency to the target tissue. We thus hypothesized that MSC sheets would therapeutically reduce kidney disease with high transplantation efficiency. When the chronic glomerulonephritis was induced by two injections of the anti-Thy 1.1 antibody (OX-7) in rats, the therapeutic efficacy of rat bone marrow stem cell (rBMSC) sheet transplantation was evaluated. The rBMSC-sheets were prepared using the temperature-responsive cell-culture surfaces and transplanted as patches onto the surface of two kidneys of each rat at 24 h after the first injection of OX-7. At 4 weeks, retention of the transplanted MSC-sheets was confirmed, and the animals with MSC-sheets showed significant reductions in proteinuria, glomerular staining for extracellular matrix protein, and renal production of TGFß1, PAI-1, collagen I, and fibronectin. The treatment also ameliorated podocyte and renal tubular injury, as evidenced by a reversal in the reductions of WT-1, podocin, and nephrin and by renal overexpression of KIM-1 and NGAL. Furthermore, the treatment enhanced gene expression of regenerative factors, and IL-10, Bcl-2, and HO-1 mRNA levels, but reduced TSP-1 levels, NF-kB, and NAPDH oxidase production in the kidney. These results strongly support our hypothesis that MSC-sheets facilitated MSC transplantation and function, and effectively retarded progressive renal fibrosis via paracrine actions on anti-cellular inflammation, oxidative stress, and apoptosis and promoted regeneration.

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Yoon, Jong Hoon, Ho Sung Lee, Yeong Moo Yi, and Young Soon Jang. "Finite Element Analysis on Superplastic Blow Forming of Ti-6Al-4V Multi-Sheets." Materials Science Forum 546-549 (May 2007): 1361–66. http://dx.doi.org/10.4028/www.scientific.net/msf.546-549.1361.

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Superplastic blow forming with diffusion bonded sheet is an effective forming technology for the production of multi-cell structures which should have light weight and high stiffness for aerospace purpose. In the current study, finite element analysis on superplastic blow forming process has been carried out in order to improve the forming process when manufacturing axi-symmetric multi-cell structures using diffusion bonded Ti-6Al-4V multi-sheets. The simulation focused on the reduction of forming time and obtaining finally required shape throughout investigating the deformation mode of sheet according to the forming conditions, which are diffusion bonding pattern and die geometry. To reduce forming time, a preforming die was required, and to obtain the final shape the bonding pattern should be also modified within allowable geometrical margin, so that the sheet is easy to deform. Moreover, an intermediate simulation result, which was forming pressure profile, was employed in real forming test to check if the prediction was reasonably on progress. In the future, a study on the thickness ratio between each sheet should be followed to obtain optimum process parameters.

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Tang, Zhonglan, Akihiko Kikuchi, Yoshikatsu Akiyama, and Teruo Okano. "Novel cell sheet carriers using polyion complex gel modified membranes for tissue engineering technology for cell sheet manipulation and transplantation." Reactive and Functional Polymers 67, no. 11 (2007): 1388–97. http://dx.doi.org/10.1016/j.reactfunctpolym.2007.07.058.

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Stankova, Lubica, Anna Kutová, Martina Doubkova, et al. "Cellulases and their promising use in tissue engineering and cell sheet technology." Materials & Design 254 (May 25, 2025): 114111. https://doi.org/10.1016/j.matdes.2025.114111.

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This study explores an innovative method for creating <strong>scaffold-free sheets of human keratinocytes</strong> using <strong>bacterial nanocellulose (BNC)</strong> that is engineered to be <strong>enzymatically degradable.</strong>

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Feng, Yuting, Zhiwei Jiang, Yanmin Zhang, et al. "Stem-cell-derived ECM sheet–implant complexes for enhancing osseointegration." Biomaterials Science 8, no. 23 (2020): 6647–56. http://dx.doi.org/10.1039/d0bm00980f.

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