To see the other types of publications on this topic, follow the link: Corneal endothelial cell density.
Journal articles on the topic 'Corneal endothelial cell density'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 journal articles for your research on the topic 'Corneal endothelial cell density.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.
1
Mishra, Neeta, Neelam Prasad, Rekha Chotalia, Uday Bajare, and Veshal V. Madan. "Millennial Generation’s Choice of Contact Lens as the Preference Over Spectacles: A Study of Corneal Endothelial Cell Density." Medical Journal of Dr. D.Y. Patil Vidyapeeth 17, no. 5 (2024): 1004–6. http://dx.doi.org/10.4103/mjdrdypu.mjdrdypu_750_23.
Full textAbstract:
ABSTRACT Introduction: The cornea of the eye is the most vulnerable organ to external insult. Contact lens is no exception. Changes in corneal epithelium, stroma, and loss of hexagonal shape in the endothelium have been taught in contact lens classes. We wanted to understand the cell density of endothelium in prolonged contact lens wear. Methods: It was a prospective, observational, cross-sectional study. The volunteers were divided into two groups: group 1 wore their contact lenses for over 5 years and group 2 never wore contact lenses. Specular microscopy was performed using Topcon SP-3000P. Computerized morphometry was used to evaluate the mean cell density of the corneal endothelial cells. Result: A statistically significant decrease in corneal endothelial cell density was observed in contact lens wearers. Conclusion: The use of contact lenses for a prolonged period significantly decreases corneal endothelial cell density. Suitable intervention may prevent such changes in the cornea.
2
Azevedo, Mariane, Nat ndez, Lu Cargnin, et al. "Specular microscopy of the corneal endothelial cells of bovines: An ex vivo study." Open Veterinary Journal 13, no. 12 (2023): 1554. http://dx.doi.org/10.5455/ovj.2023.v13.i12.5.
Full textAbstract:
Background: The endothelium is the most posterior layer of the cornea and essential for maintaining corneal transparency. Due to variations in corneal endothelial parameters among different species, knowledge of the normal parameters for each species is crucial. Aim: To evaluate the corneal endothelium of bovines using contact specular microscopy. Methods: Twenty eyeballs from 10 male Brangus (Bos taurus) aged 24 months were evaluated. Contact specular microscopy was performed on the central corneal area. The analysed parameters were endothelial cell density (ECD) and endothelial cell morphology. Results: The ECD in the central area was 1277 cells/mm². Regarding the morphology, mainly cells with six (74.3%), five (14.7%) and seven sides (10%) were found. There were no significant differences in endothelial cell density and morphology between left and right eyes. Conclusion: Contact specular microscopy facilitated the analysis and measurement of corneal endothelial parameters in bovines. The data obtained will serve as a reference for the analysis of bovine corneal endothelium.
3
Park, Chanjoon, Hye Yeon Yoon, and Hyun Seung Kim. "Factors Influencing Corneal Endothelial Cell Density after Descemet Stripping Automated Endothelial Keratoplasty." Journal of the Korean Ophthalmological Society 65, no. 11 (2024): 699–707. http://dx.doi.org/10.3341/jkos.2024.65.11.699.
Full textAbstract:
Purpose: To identify determinants of corneal endothelial cell density at 12 months after Descemet stripping automated endothelial keratoplasty (DSAEK).Methods: This study included patients with bullous keratopathy who underwent DSAEK with internationally sourced donor corneas from March 2021 to March 2022. We assessed various preoperative factors in recipients (age, cause of bullous keratopathy, intraocular pressure, visual acuity, anterior chamber volume, depth and angle, pupil size, white-to-white corneal diameter, and preoperative corneal endothelial cell density) and donors (age, death to preservation time, preservation to transplantation time, death to transplantation time, corneal endothelial cell density, trephine size, and precut tissue thickness). We analyzed factors influencing corneal endothelial cell density up to 12 months after keratoplasty.Results: This study involved 53 patients, with an average recipient age of 65.9 ± 14.6 years prior to surgery. Donor corneas had an average endothelial cell density of 2,871.6 ± 271.6 cells/mm<sup>2</sup>. At 6 and 12 months postoperatively, endothelial cell densities had decreased by 36.9% and 47.2%, respectively, compared with preoperative levels. Multivariate linear regression analysis indicated that endothelial cell densities at 3 and 12 months postoperatively were linked to recipient age and anterior chamber depth.Conclusions: Corneal endothelial cell density after DSAEK typically stabilized after initial decline; higher densities were found in older recipients and individuals with shallower anterior chambers.
4
Pardasani, Rajiv, and Sohan Lohiya. "Study of Changes in Corneal Thickness and Corneal Endothelial Cell Density after Phacoemulsification Cataract Surgery." Journal of Evolution of Medical and Dental Sciences 10, no. 12 (2021): 866–72. http://dx.doi.org/10.14260/jemds/2021/187.
Full textAbstract:
BACKGROUND Phacoemulsification is a common surgery for cataract. The endothelium comprises of hexagonal cells which in single layer is essential for maintaining the transparency of the cornea. We wanted to compare endothelial cell density (ECD), central corneal thickness (CCT), coefficient of variability, and hexagonality of endothelial cells before and after phacoemulsification surgery. we also wanted to evaluate the endothelial cell loss after phacoemulsification surgery. METHODS A rural hospital-based prospective observational study with 120 patients was conducted in a hospital based setting utilising the data of patient’s eye by considering inclusion and exclusion criteria, before and after phacoemulsification surgery by using noncontact specular microscope. RESULTS The mean endothelial cell density significantly decreased postoperatively at day one, 4th week, 12th week. The mean central corneal thickness increased significantly at postoperative day one, then subsequently decreased at postoperative 4th week and 12th week (P value = 0.0001), but never reached the preoperative value. There was a significant change in coefficient of variation and hexagonality postoperatively (P value = 0.0001). CONCLUSIONS The primary result is the change in corneal endothelial cell density (cells per square millimetre of the corneal surface) which is decreased, and the central corneal thickness calculated in micro meter is increased. As endothelial cells do not replicate, to reimburse cell loss there are changes in coefficient of variation and hexagonality after phacoemulsification surgery. KEY WORDS Corneal Endothelial Cell Density, Central Corneal Thickness, Coefficient of Variation, Hexagonality, Phacoemulsification, Specular Microscope
5
Kareem, Alyaà, and Giyath Aldeen Neamah. "CORNEAL ENDOTHELIOPATHY IN PSEUDOEXFOLIATION SYNDROME." Iraqi Journal of Medical Sciences 16, no. 1 (2018): 57–65. http://dx.doi.org/10.22578/ijms.16.1.9.
Full textAbstract:
Background: Pseudoexfoliation syndrome is a common disorder with a wide range of ophthalmic presentation and risks. Corneal endotheliopathy is one of these presentations. Objective: To evaluate corneal endothelial cell morphology, density and function in eyes with pseudoexfoliation syndrome. Methods: 120 eyes of sixty patients with clinically evident unilateral pseudoexfoliation were examined with non-contact specular microscopy (SP-3000P) Topcon Corporation. Central corneal thickness (T), cell density (CD), and percentage of cell hexagonality (HEX%) were measured and the values of the affected eyes were compared to those of the fellow normal eyes. Corneas with CD less than 2000/mm2 or HEX% <50% were considered as at-risk corneas for decompensation. The pseudoexfoliated eyes were subdivided into three groups according to the density of the pseudoexfoliated material (those with deposits on the lens capsule alone, on the pupil margin alone or on both) and the morphometric values of endothelial cells in each group were studied. Statistical analysis was performed using a 2-tailed Student t-test and the Chi square test, P value <0.05 was considered significant. Results: Significant increase in central corneal thickness (500.25±28.95 micron vs 493.18±28.59 micron) and reduction in endothelial cell density (2307.5±272.3 vs 2480.2±289.9 cell/mm2) with a non- significant decrease in cell hexagonality (51.78±8.9 vs 53.48±6.2 %) was noticed in eyes affected by pseudoexfoliation syndrome as compared to the contralateral normal eyes. The changes were noticed more when the severity of the condition increases as reflected by the density of the pseudoexfoliated material. At risk corneas were found more frequently in eyes with pseudoexfoliation based on both endothelial cell density and cell hexagonality. Conclusion: Pseudoexfoliation syndrome is a cause of corneal endotheliopathy and a risk for corneal decompensation. More endothelial cell changes are found in eyes with advanced pseudoexfoliation. Extra care and more meticulous handling is required while operating upon eyes with pseudoexfoliation to reduce the risk for corneal decompensation. Keywords: Pseudoexfoliation, corneal endothelium, endotheliopathy, decompensation. Citation: Kareem AA, Neamah GAT. Corneal endotheliopathy in pseudoexfoliation syndrome. Iraqi JMS. 2018; Vol. 16(1): 57-65. doi: 10.22578/IJMS.16.1.9
6
Singasandra, Sanjana Marijogaiah, Radhika Srinivasagupta Mohan, Dakshayini Mallaiah, and Shwetha Bennavara Venkataswamy. "A comparative study of endothelial cell loss in small incision cataract surgery versus phacoemulsification cataract surgery by using specular microscope at tertiary care ophthalmic centre in Bengaluru, Karnataka." IP International Journal of Ocular Oncology and Oculoplasty 7, no. 4 (2022): 399–405. http://dx.doi.org/10.18231/j.ijooo.2021.084.
Full textAbstract:
Most commonly performed surgical procedure in Ophthalmology is cataract extraction by MSICS and Phacoemulsification. It has always been associated with damage to endothelium of cornea, a layer which is vital for maintaining corneal transparency. The corneal endothelium regulates stromal hydration and maintains the transparency of the cornea by constantly removing the fluid out of the corneal stroma. The density of corneal endothelial cells and its integrity is an important determinant for corneal transparency. Post-operative corneal decompensation leading to reduced visual acuity can occur as a result of this surgical trauma. Hence it is necessary to determine the surgical technique safest to corneal endothelium. Specular microscopy helps to determine this corneal endothelial cell density. A hospital based longitudinal study was done from November 2017 to May 2019 in 124 patients at Minto Ophthalmic Hospital. 62 patients underwent MSICS and 62 phacoemulsification. After a written informed consent patients were evaluated with detailed history, slit lamp examination, direct and indirect ophthalmoscopy, biometry, lacrimal syringing, IOP measurement with tonometry and endothelial cell count was evaluated using non contact specular microscopy preoperatively and postoperative 1 week and 6 weeks. Statistical data analysed by unpaired – t test. The mean ECC (cells/mm) in MSICS and phacoemulsification group preoperatively was 2486.82 + 152.730 and 2433.71 + 192.692 respectively. The mean endothelial cell loss (cells/mm) was 314.61 + 64.428 and 324.31 + 30.67 at 1 week and 345.71 + 66.68 and 354.95 + 53.885 at 6 weeks postoperatively between the two groups. The endothelial cell loss was not statistically significant at 1 week (p -0.28) and 6 weeks (p-0.39) postoperatively between the two groups. There was no clinically or statistically significant difference in the endothelial cell loss between MSICS and Phacoemulsification. As MSICS is economical and less dependent on technology, it can be a safe option in developing world.
7
Ghazanfar, Qurat ul Ain, Omar Zafar, Noman Nazir Ahmed, and Muhammad Manzoor Awan. "Comparison of Corneal Endothelium in Patients with Uveitis and Healthy Subjects Using Specular Microscopy." Pakistan Armed Forces Medical Journal 72, no. 5 (2022): 1510–13. http://dx.doi.org/10.51253/pafmj.v72i5.3168.
Full textAbstract:
Objective: To evaluate changes in corneal endothelium in patients with anterior uveitis by using specular microscopy and compare it with the corneal endothelium of healthy subjects.
 Study Design: Comparative cross-sectional study.
 Place and Duration of Study: Armed Forces Institute of Ophthalmology, Rawalpindi Pakistan, from Jun 2018 to Jun 2019.
 Methodology: This study included 65 eyes of 65 patients with anterior uveitis and 65 eyes of healthy subjects. Corneal endothelium was assessed in all participants of both Groups by using Specular microscopy. Corneal endothelial markers like central corneal thickness (CCT), cell density (CD), cell minimum area, cell maximum area, the average of cell size, percent of hexagonality (HEX%), coefficient of variation (CV) were compared in both Groups. The Uveitis Group was further subdivided into active and inactive Uveitis Subgroups, and corneal endothelium markers were also compared in these Subgroups.
 Results: The mean endothelial cell density was 2626.0±413.0 cells/mm² in patients with uveitis, whereas cell density was 2766.0±327.0 cells/mm² in healthy subjects. The difference in endothelial cell density was statistically significant between the Groups (p=0.03). The difference between central corneal thickness, maximum cell area, average cell size and hexagonality values between Groups was also statistically significant, whereas there was no difference in terms of cell minimum area between these Groups. There was no statistically significant difference between inactive and active uveitis when corneal endothelial markers were compared between them (p>0.05).
 Conclusion: There was an effect of uveitis on central corneal thickness, endothelial cell density, cell size, and morphology compared to healthy subjects,......
8
Marwah Mahammed Moftah and Yasmena Mahmod Abdulhadi. "Effect of Primary Pterygium on Endothelial Cell Density." Journal of Medical and Health Studies 5, no. 2 (2024): 113–18. http://dx.doi.org/10.32996/jmhs.2024.5.2.14.
Full textAbstract:
Pterygium is a proliferation of fibrovascular tissue over the cornea, causing inflammation and neovascularization. Risk factors include genetic predisposition and chronic environmental irritation. Four grades exist, affecting the corneal limbus, pupil, and pupillary area. Pterygium formation causes flattening of the horizontal meridian and changes in corneal layers to determine if there is a correlation between primary Pterygium and a decrease in corneal endothelial cell density (ECD). This cross-sectional random observational comparative study analyzed 50 patients at Benghazi Teaching Eye Hospital from December 2023 to February 2024. Patients with unilateral pterygium were included. The study assessed endothelial cell count using non-contact specular microscopy and analyzed the corneal endothelial parameters using a Topcon Specular Microscope. The study participants were 74% male, with a mean age of 53.74 +11.06 years. Pterygium grading revealed that 36% had grade one, 62% had grade two, and only 2% had grade three. The study found a significant difference in endothelial cell density between the case and control groups(p<0.001), with the case group having a lower mean ECD than the control group. Central corneal thickness was also significantly different between eyes with primary pterygium invasion and those without(p<0.001). However, the Kruskal-Wallis Test showed no significant association between pterygium grading and endothelial cell density(p>0.05). Primary pterygium significantly affects corneal endothelial cell density and central corneal thickness. Further research is needed to understand its clinical implications and correlation with pterygium progression. Understanding pterygium's impact on corneal health and the regularity of astigmatism could improve management and treatment options.
9
Anton Steindor, Friedrich, Céline Trigaux, Christoph Holtmann, Kristina Spaniol, Gerd Geerling, and Maria Borrelli. "Preserflo MicroShunt: Efficacy and Endothelial Cell Density." Journal of Glaucoma 32, no. 12 (2023): 1018–21. http://dx.doi.org/10.1097/ijg.0000000000002325.
Full textAbstract:
Précis: A main safety concern associated with new glaucoma implants is corneal endothelial cell loss, which could even be observed in already established procedures. Purpose: The purpose of this study was to evaluate the efficacy and corneal safety, especially corneal endothelial cell loss (ECL), after Preserflo MicroShunt implantation. Methods: A monocentric, prospective clinical observational study of patients with a follow-up of at least 12 months who underwent Preserflo MicroShunt implantation at the Department of Ophthalmology, University Hospital of Düsseldorf, Germany, between January 2020 and October 2021. Endothelial cell density (ECD), the distance between the tip of the implant and the corneal endothelium, and the success of the surgery were analyzed. Results: In total, 14 eyes of 12 patients were included. After a mean follow-up of 20±2.7 months intraocular pressure was significantly reduced by 8.2 mm Hg (P=0.0041); in 28.6% of patients, a revision surgery was necessary. Reduction of preoperative and follow-up ECD was not statistically significant (ECL of 45 cells/mm2, ECDpreoperative=2074±703.6 cells/mm2, ECDlast follow-up=2029±742.3 cells/mm2, P=0.42). A longer intracameral shunt tube length correlated significantly with a higher distance between the shunt tip and corneal endothelium (r=0.61, P=0.036). Conclusions: Preserflo MicroShunt effectively lowered intraocular pressure without substantial ECL after a minimum follow-up period of 17 months.
10
Ting, Darren S. J., Gary S. L. Peh, Dawn J. H. Neo, et al. "Cell Counting and Cell Cycle Analysis of Simple Non-Cultured Endothelial Cell Injection (SNEC-I) Therapy: Characterization for Clinical Translation." Cells 14, no. 13 (2025): 986. https://doi.org/10.3390/cells14130986.
Full textAbstract:
Human corneal endothelial cell therapy has recently emerged as a novel solution to treat corneal endothelial diseases. We previously demonstrated the potential of utilizing non-cultured primary corneal endothelial cells (CEnCs) isolated from donor corneas with low endothelial cell density for simple non-cultured endothelial cell injection (SNEC-I) therapy. This study aimed to develop a robust and semi-automated approach for cell counting, characterize the extent of cellular manipulation, and evaluate the translational workflow. To address this, we evaluated manual and automated cell counting approaches and characterized the extent of manipulation of CEnCs through the analysis of cell cycle status, gene expressions, and transcriptomic profiles with single-cell RNA-sequencing. The translational feasibility and functionality of SNEC-I therapy were examined using an established rabbit model of bullous keratopathy. Manual hemocytometry and automated cell-counters exhibited comparable accuracy and reproducibility. Analysis of cell cycle status, cell cycle genes (n = 11), and transcriptomic profiles revealed close resemblance between the native corneal endothelium and its donor-matched SNEC-I-harvested cells. Successful resolution of bullous keratoplasty in the pre-clinical model supports the feasibility, efficacy, and safety of SNEC-I therapy. In conclusion, SNEC-I therapy serves as an attractive corneal endothelial therapeutic approach (from a regulatory standpoint) in view of the minimal extent of cellular manipulation.
11
Sicks, Ben, Martin Hessling, Kathrin Stucke-Straub, Sebastian Kupferschmid, and Ramin Lotfi. "Disinfection of Human and Porcine Corneal Endothelial Cells by Far-UVC Irradiation." Medicina 61, no. 3 (2025): 416. https://doi.org/10.3390/medicina61030416.
Full textAbstract:
Background and Objectives: The cornea protects the eye from external influences and contributes to its refractive power. Corneas belong to the most frequently transplanted tissues, providing a last resort for preserving the patient’s vision. There is a high demand for donor corneas worldwide, but almost 4% of these transplants are not eligible due to microbial contamination. The objective of this study is to ascertain the suitability of 222 nm Far-UVC irradiation for the decontamination of corneas without damaging corneal endothelial cells. Materials and Methods: To assess the destructive effect of irradiation and, thus, identify the applicable dose needed to decontaminate the cornea without interfering with its integrity, 141 porcine corneas were irradiated with 0, 60 or 150 mJ/cm2 at 222 nm. In the second step, a series of 13 human corneas were subjected to half-sided irradiation using 15 or 60 mJ/cm2 at 222 nm. After five days of in vitro culturing, the endothelial cell density of the non-irradiated area of each human cornea was compared to the irradiated area. Results: Irradiation with up to 60 mJ/cm2 had no detectably significant effect on the cell integrity of human corneas (p = 0.764), with only a minimal reduction in cell density of 3.7% observed. These findings were partially corroborated by tests on porcine corneas, wherein the variability between test groups was consistent, even at increased irradiation doses of up to 150 mJ/cm2, and no notable effects on the irradiated porcine endothelium were monitored. The efficacy of the antimicrobial treatment was evident in the disinfection tests conducted on corneas. Conclusions: These initial irradiation experiments demonstrated that 222 nm Far-UVC radiation has the potential to decontaminate the cornea without compromising sensitive endothelial cell viability.
12
Ganekal, Sunil, and Varun Ganekal. "Anterior capsule sparing parsplana lensectomy in cataract with severely compromised corneal endothelium." Indian Journal of Clinical and Experimental Ophthalmology 8, no. 2 (2022): 224–27. http://dx.doi.org/10.18231/j.ijceo.2022.045.
Full textAbstract:
To evaluate the results of parsplana lensectomy in the management of cataract with severely compromised or abnormal corneal endothelium. Retrospective interventional case study comprised 22 patients (9 women, 13 men; 22 eyes) with severely abnormal corneal endothelium ut still had transparent cornea. Of which 14 had history of penetrating keratoplasty, 6 had history of trabeculectomy and 2 were diagnosed with Fuch’s endothelial dystrophy. Postoperative est corrected visual acuity (BCVA), mean density of corneal endothelial cell and other complications were analyzed. Mean follow-up period was 17.2 months (ranging from 6 to 31 months). Postoperative BCVA were found to be 6/60 or below in 2 patients, 6/60-6/18 in 7 patients and 6/18-6/12 in 7 patients and 6/12 or better in 6 patients. BCVA improved in all patients after surgery, except in one patient with Fuch’s endothelial dystrophy BCVA turned worse again about five months after surgery and eventually patient developed bullous keratopathy. The mean density of corneal endothelial cells was 1018± 84/ mm (range 844/ mm to 1176/ mm) before operation and 981±93/ mm (range 853 to 1211/ mm) after operation. No significant difference in the density of corneal endothelial cells was found among the patients before and after operation (P=0.43).Pars plana lensectomy is able to decrease surgically-induced corneal endothelial damage and is safe in the management of cataract with severely compromised corneal endothelium.
13
Ostrovsky, D. S., S. A. Borzenok, B. E. Malyugin, O. P. Antonova, M. Kh Khubetsova, and T. Z. Kerimov. "Preparation and evaluation of a suspension of human corneal endothelial cells isolated from the eyes of cadaveric donors for transplantation in an <em>ex vivo</em> experiment." Russian Journal of Transplantology and Artificial Organs 26, no. 1 (2023): 103–12. http://dx.doi.org/10.15825/1995-1191-2024-1-103-112.
Full textAbstract:
Background. According to the World Health Organization, corneal diseases are one of the major causes of blindness globally. Endothelial dystrophy is one of the etiological factors leading to corneal diseases. The corneal endothelium is a monolayer of cells with virtually no mitotic activity. When the density of corneal endothelial cells falls below a critical threshold, the endothelium loses its ability to regulate corneal stromal hydration. This leads to corneal clouding and, consequently, to reduced visual acuity and quality of life of the patient. In this regard, various keratoplasty methods are widely used in clinical practice. Today, it is technically possible to transplant all corneal layers via penetrating keratoplasty, and to transplant the posterior epithelium via layer-bylayer keratoplasty. These surgical approaches are now widely used in everyday practice, but they require the use of scarce material – cadaveric donor corneas, from which grafts for the above-mentioned operations are formed in the conditions of an eye bank. In this regard, protocols for obtaining human corneal endothelial cell (HCEC) culture for subsequent transplantation have been proposed in recent years. However, the use of such approaches in Russia is limited by the law. The aim of this study was to experimentally justify the possibility of transplanting uncultured endothelial cells, isolated from cadaveric human corneas. Materials and methods. The first stage of the work consisted of obtaining a suspension of endothelial cells from cadaveric donor corneas and studying it; at the second stage, the transplantation effectiveness of the resulting cell suspension was assessed in an ex vivo experiment. Results. The cell phenotype after transplantation by the proposed method had high viability and preservation. Conclusions. The presented results suggest that phenotype and adhesion ability are preserved, and that the cell suspension has a high level of viability under adequate loss of endothelial cells during transplantation in the ex vivo experiment.
14
Khodair, Aya A. I., Mouhamed Elsaadany Ghoraba, Rabab M. Elseht, and Dina Tadros. "Effect of intracameral injection of ceftazidime on corneal endothelium by specular microscope after uncomplicated phacoemulsification cataract surgery." Journal of the Egyptian Ophthalmological Society 117, no. 1 (2024): 59–62. http://dx.doi.org/10.4103/ejos.ejos_85_23.
Full textAbstract:
Background Loss of endothelial cells is inevitable after cataract surgery. Therefore, it is critical to protect the corneal endothelium during cataract surgery to reach good visual outcomes afterward. Maintenance of corneal clarity depends on the good function of healthy endothelial cells in addition to an intact pumping system intended to remove fluid from the corneal stoma. Purpose Our study aimed to assess changes in the corneal endothelium using specular microscopy, after intracameral injection of ceftazidime in uncomplicated phacoemulsification and compared with a placebo. The changes were measured preoperatively and 1 month postoperatively. Patients and methods Our prospective cohort study comprised 40 eyes from 35 patients diagnosed with cataract, all of whom underwent uncomplicated phacoemulsification for cataract extraction. Results Endothelial cell density significantly decreased postoperatively compared with preoperative values in both groups. Central thickness of the cornea, coefficient of variation, and visual acuity significantly increased postoperatively compared to preoperative values in both groups. Endothelial cell density showed a significant decrease postoperatively with a P value equal to 0.01 for group A (placebo group) and 0.02 for group B (ceftazidime group). Central thickness of the cornea, coefficient of variation, and visual acuity showed significant increases postoperatively with a P value equal to 0.026, 0.005, and 0.001 for group A and 0.001, 0.034, and 0.001 for group B, respectively. Conclusion Ceftazidime can be considered safe for corneal endothelium when used intracamerally during phacoemulsification cataract surgery for prophylaxis of postoperative endophthalmitis.
15
Şahin Vural, Gözde, and Ferhat Çay. "The comparison of corneal endothelium morphology through specular microscopy in morbid obesity with healthy controls." Journal of Comprehensive Surgery 1, no. 4 (2023): 78–81. http://dx.doi.org/10.51271/jocs-0019.
Full textAbstract:
Aims: To evaluate the morphological changes in corneal endothelial cells with morbid obesity, and to analyze the relationship between BMI and corneal endothelial features. Methods: The central corneal thickness (CCT), the average endothelial cell density (ECD) (cell/mm²), average cell area (CA) (µm²), coefficient of variation of cell area (CV)- (CV: Standard deviation/CA) values were recorded via specular microscopy in morbidly obese patients and controls. The whole parameters were compared. Results: The morbid obese patients had significantly thicker corneas (p=0.013), and significant loss of hexagonality (p=0.012) compared with controls, while there was no significant difference in ECD (p=0.311), CA (p=0.292), and CA (p=0.161). In linear regression analysis, there was no significant correlation between CCT, abdominal circumference, and the endothelium parameters. Conclusion: Although BMI has the effect of changing corneal morphology, this change is not correlated with an increase in BMI.
16
Perwitasari, Birgitta Henny, Evelyn Komaratih, and Dicky Hermawan. "Literature Review: The Role of Adipose Stem Cell Secretome In Caspase-3 Regulation and Endothelial Cell Density Preservation in Corneal Regeneration Post-Phacoemulsification." Journal of Neonatal Surgery 14, no. 14S (2025): 728–32. https://doi.org/10.63682/jns.v14i14s.4037.
Full textAbstract:
Phacoemulsification is the most widely used surgical technique for cataract removal; however, it poses risks of corneal endothelial damage, leading to complications such as corneal edema and vision impairment. The corneal endothelium plays a crucial role in maintaining corneal transparency, yet it has limited regenerative capacity. Endothelial cell loss following phacoemulsification results from mechanical trauma, oxidative stress, and apoptosis, with caspase-3 acting as a key mediator of programmed cell death. Excessive apoptosis leads to a critical reduction in endothelial cell density (ECD), causing corneal decompensation and potential vision loss. Current treatments, including endothelial keratoplasty, are limited by donor shortages and surgical risks. Stem cell-derived secretomes, particularly from adipose-derived stem cells (ASCs), have emerged as a promising therapeutic approach for corneal endothelial repair. ASC secretomes contain bioactive molecules such as growth factors and cytokines that promote endothelial cell survival, reduce apoptosis by downregulating caspase-3, and enhance tissue regeneration. Preclinical studies suggest that ASC secretomes may effectively support endothelial recovery post-phacoemulsification. This literature review explores the mechanisms of endothelial damage, the role of apoptosis in corneal cell loss, and the therapeutic potential of ASC secretomes as a novel intervention for protecting and restoring corneal endothelium following cataract surgery. Additionally, the use of New Zealand white rabbits as an animal model for studying endothelial regeneration is discussed.
17
Siska, Siska, Gede Eka Wiratnaya, I. Made Bakta, et al. "The Role of Mesenchymal Stem Cells for Corneal Endothelial Regeneration: A Systematic Review." Rambam Maimonides Medical Journal 15, no. 4 (2024): e0017. http://dx.doi.org/10.5041/rmmj.10531.
Full textAbstract:
Objective: A single layer of tightly spaced cells, known as the endothelium, rests on the posterior side of the cornea. This endothelium regulates the stroma’s relative dehydration, which is essential for corneal clarity. Cell therapy is an innovative method being used to repair various corneal abnormalities. Mesenchymal stem cells (MSCs) are now one of the most significant types of stem cells scientists have studied. This study aimed to evaluate the role of MSCs for corneal endothelial regeneration. Methods: A systematic review was performed by searching for articles from reputable databases with many study-type references, including PubMed, Cochrane Library, Science Direct, and Google Scholar, up to January 2024. The resulting data were displayed using the 2020 PRISMA flowchart and evaluated using the PRISMA 2020 checklist. Most of the included studies were in vivo and used topical application and anterior chamber injection as the administration routes. Results: Based on the findings of this review, MSCs increased corneal endothelial cell density, improved the defect area and corneal transparency, facilitated endothelial cell regeneration and wound healing, and decreased neovascularization and corneal pro-inflammatory cytokines as compared to controls. Conclusion: Administration of MSCs into the anterior chamber could increase regeneration and proliferation of corneal endothelial tissue.
18
Wu, Chun-Yen, Da-Fong Song, Zhi-Jia Chen, Chao-Sheng Hu, David Pei-Cheng Lin, and Han-Hsin Chang. "Absence of the Klotho Function Causes Cornea Degeneration with Specific Features Resembling Fuchs Endothelial Corneal Dystrophy and Bullous Keratopathy." Biology 13, no. 3 (2024): 133. http://dx.doi.org/10.3390/biology13030133.
Full textAbstract:
The Klotho loss-of-function mutation is known to cause accelerated senescence in many organs, but its effects on the cornea have not been published. The present study aims to investigate the effects of the Klotho null mutation on cornea degeneration and to characterize the pathological features. Mouse corneas of Klotho homozygous, heterozygous, and wild-type mice at 8 weeks of age for both genders were subject to pathological and immunohistological examinations. The results show an irregular topography on the corneal surface with a Klotho null mutation. Histological examinations revealed a reduced corneal epithelial cell density, endothelial cell-shedding, and decreased cornea stromal layer thickness in the absence of the Klotho function. Furthermore, guttae formation and the desquamation of wing cells were significantly increased, which was comparable to the characteristics of Fuchs endothelial corneal dystrophy and bullous keratopathy. The mechanism analysis showed multi-fold abnormalities, including oxidative stress-induced cornea epithelium apoptosis and inflammation, extracellular matrix remodeling in the stroma, and a disruption of epithelial repair, presumably through the epithelial–mesenchymal transition. In conclusion, cornea degeneration was observed in the Klotho loss-of-function mutant mice. These pathological features support the use of Klotho mutant mice for investigating age-related cornea anomalies, including Fuchs endothelial corneal dystrophy, bullous keratopathy, and dry eye diseases.
19
Shen, Lin, Peng Sun, Liqun Du, et al. "Long-Term Observation and Sequencing Analysis of SKPs-Derived Corneal Endothelial Cell-Like Cells for Treating Corneal Endothelial Dysfunction." Cell Transplantation 30 (January 1, 2021): 096368972110178. http://dx.doi.org/10.1177/09636897211017830.
Full textAbstract:
Corneal endothelial dysfunction is a principal cause of visual deficiency. Corneal transplantation is the most effective treatment for corneal endothelial dysfunction. However, a severe shortage of available donor corneas or human corneal endothelial cells (HCECs) remains a global challenge. Previously, we acquired corneal endothelial cell-like cells (CEC-like cells) derived from human skin-derived precursors (SKPs). CEC-like cells were injected into rabbit and monkey corneal endothelial dysfunction models and exerted excellent therapeutic effect. In this study, we prolonged the clinical observation in the monkey experiment for 2 years. Polymerase chain reaction (PCR) and DNA sequencing were carried out to confirm the existence of CEC-like cells. Histological examinations were carried out to show the corneal morphology. Further transcriptome sequencing was also carried out on HCEC, CEC-like cells before transplantation and after transplantation. We found that the monkeys cornea remained transparent and normal thickness. The total endothelial cell density decreased gradually, but tended to be stable and remained in a normal range during 2-year observation. The CEC-like cells persist during observation and could adapt to the microenvironment after transplantation. The gene expression pattern of CEC-like cells was similar to HCEC and changed slightly after transplantation. In conclusion, this study presented a brand-new insight into CEC-like cells and further provided a promising prospect of cell-based therapy for corneal endothelial dysfunction. The renewable cell source, novel derivation method and simple treatment strategy may be clinically applied in regenerative medicine in the future.
20
Rahmawati, Farahdina, Wasisdi Gunawan, Retno Ekantini, Rinanto Prabowo, and Indra Tri Mahayana. "Density and morphology of corneal endothelial cell after phacoemulsification using ringer lactate versus balanced salt solution as irrigating solutions." International Eye Research 1, no. 2 (2020): 72–77. http://dx.doi.org/10.18240/ier.2020.02.02.
Full textAbstract:
AIM: To compare the difference in corneal endothelial cell density and morphology after phacoemulsification using ringer lactate (RL) and balanced salt solution (BSS) irrigating solutions. METHODS: The prospective randomized controlled trial study was conducted between February 2017 and April 2017 in Dr. YAP Eye Hospital, Yogyakarta, Indonesia. There were a total of 52 subjects (52 eyes) who were senile cataract patients further grouped into two, 26 patients undergoing the phacoemulsification procedure using RL irrigating solution and the other 26 patients with BSS irrigating solution, both conducted by one operator. On the 1, 7, and 28d post operative, an evaluation was done to measure the density and corneal endothelial cell morphology, as well as the variable of inflammation in the two groups. RESULTS: Fifty-two eyes had undergone phacoemulsification with posterior intraocular lens implantation. Both groups were evaluated for the endothelial cell reduction and corneal endothelial cell morphology change, along with postoperative inflammation. On the 28d postoperative, endothelial cell reduction in the BSS group (173.96 cell/mm2, 8.12%) was lower than the RL group (253.20 cell/mm2, 10.25%), percentage of corneal endothelial cell variation coefficient increase in the BSS group (2.92%, 8.36%) was lower compared to the RL group (3.42%, 9.96%), decrease of hexagonal cells of corneal endothelium cells presentation percentage in the BSS group (4.30%, 8.17%) was lower compared to the RL group (4.84%, 8.97%), and the percentage increase of central corneal thickness in the BSS group (4.69 μm, 0.89%) was almost equal to the RL group (4.53 μm, 0.90%). All of the results regarding difference in density and corneal cell endothelium morphology between the two groups did not reveal any statistically significant difference (P>0.05). Inflammatory variable in the two groups were even. CONCLUSION: BSS and RL were equal in their capability of maintaining endothelial cell loss and endothelial cell morphologic change in senile cataract patients after phacoemulsification.
21
Shen, Yun-Zhi, Mi Xu, and Song Sun. "In Vivo Confocal Microscopy Observation of Cell and Nerve Density in Different Corneal Regions with Monocular Pterygium." Journal of Ophthalmology 2020 (March 23, 2020): 1–7. http://dx.doi.org/10.1155/2020/6506134.
Full textAbstract:
Purpose. To investigate the effects of pterygium on corneal cell and nerve density in patients with unilateral pterygium using in vivo laser scanning confocal microscopy (LSCM). Methods. In this cross-sectional study, 24 patients with unilateral pterygium who were treated in the Department of Ophthalmology of the Second People’s Hospital of Wuxi City from April 2018 to July 2018 were analyzed. Each eye with pterygium and its fellow eye were imaged by LSCM. The density of basal corneal epithelial cells, anterior stromal cells, posterior stromal cells, dendritic cells, and endothelial cells in pterygium and adjacent clear cornea was measured. In the fellow eyes, the central cornea, nasal cornea, nasal mid-peripheral cornea, and temporal cornea were imaged. The difference in the density of cells and subepithelial nerve fibers in different corneal regions of eyes with pterygium was analyzed. The cell and nerve density of the fellow cornea were also measured to exclude the influencing factors. Results. The density of corneal basal epithelial cells in the central corneas of eyes with pterygium was 6497 ± 1776 cells/mm2, which was higher than that in the area near the head of pterygium (5580 ± 1294 cells/mm2, P<0.001), the region above pterygium (6097 ± 1281 cells/mm2, P=0.049), and the region below pterygium (5463 ± 1007 cells/mm2, P=0.001). The density of anterior stromal cells in the central cornea was 742 ± 243 cells/mm2, which was higher than that in the area near the head of pterygium (587 ± 189 cells/mm2, P=0.005), the region below pterygium (492 ± 159 cells/mm2, P=0.005), and the temporal cornea (574 ± 164 cells/mm2, P=0.003). The density of endothelial cells in the central cornea was 2398 ± 260 cells/mm2, which was higher than that in the area near the head of pterygium (2296 ± 231 cells/mm2, P=0.011) and the region below pterygium (2272 ± 400 cells/mm2, P=0.020). The density of dendritic cells in the central cornea was 53 ± 48 cells/mm2, which was lower than that in the area near the head of pterygium (250 ± 224 cells/mm2, P=0.001), the upper region (103 ± 47 cells/mm2, P=0.006), and the lower region (90 ± 48 cells/mm2, P=0.023). The corneal nerve fiber length (CNFL) in the center was higher than that in the area near the head of pterygium, the upper region, and the lower region. Compared with fellow eyes, eyes with pterygium had a significantly higher mean corneal power (KM) (P<0.001). There was a significant positive linear relationship between the corneal area invaded by pterygium of pterygia and KM (r = 0.609, P=0.009). Conclusion. Basal epithelial cells, stromal cells, endothelial cells, dendritic cells, and subepithelial nerve fibers in the central cornea of eyes with pterygium were different from those of pterygium and adjacent clear cornea. LSCM is effective for observing the morphology and quantity of corneal cells in pterygium.
22
Patel, Dhaval, Radhika Tandon, Anita Ganger, Aarti Vij, Sanjeev Lalwani, and Adarsh Kumar. "Study of death to preservation time and its impact on utilisation of donor corneas." Tropical Doctor 47, no. 4 (2017): 365–70. http://dx.doi.org/10.1177/0049475517713406.
Full textAbstract:
To evaluate the impact of death-to-preservation time (DPT) on effective utilisation of donor corneas. In a prospective observational study conducted at our tertiary eye centre, donated corneas received over a 15-month period from November 2011 to January 2013 were evaluated. Donor age, donor refrigeration (done or not), DPT, endothelial cell density (ECD), corneal grading, clinical utilisation and surgical outcome after graft transplantation were noted. To analyse the impact of different DPT on donor cornea transplantation, primary outcome measures (corneal grading and endothelial cell density) and secondary outcome measures (primary graft failure and graft infection) were analysed. A total of 990 corneas were assessed. Primary outcomes showed no significant difference for higher DPT ( P > 0.01). ECD, where DPT was >12 h, was better for refrigerated corneas ( P < 0.001). Prolonged DPT had no significant effect on primary graft failure ( P = 0.131) and graft infection ( P = 0.137) in the first month after transplantation. We find that DPT should not be the only criteria to assess the cornea quality; other donor characteristics should be considered equally important. Donor refrigeration should be encouraged in cases where early retrieval is not possible.
23
Rasheed, Ahmed, and Diyar Kadum. "CORNEAL CHANGES IN A SAMPLE OF PSEUDOEXFOLIATION IRAQI PATIENTS." Iraqi Journal of Medical Sciences 16, no. 3 (2018): 232–38. http://dx.doi.org/10.22578/ijms.16.3.2.
Full textAbstract:
Background: Pseudoexfoliation syndrome is a common eye disorder that can affect different parts of the eye causing significant morbidity. Objective: To compare corneal endothelial changes between patients with pseudoexfoliation syndrome and normal age matched patients. Methods: Specular microscopy was used to measure central corneal thickness, average size of endothelial cells and coefficient of variation in cell area, endothelial cell density, as well as hexagonality of 238 eyes from 238 patients (120 eyes from patients with pseudo exfoliation syndrome and 118 eyes from normal age-matched patients). Results: Patients with pseudoexfoliation syndrome had lower central corneal thickness (p value 0.049) compared to the control (491.6±28.86 vs 502.5±35.77 µm), lower endothelial cell density (p value 0.02) compared to control (2458.9±430.8 vs 2585.3±378.8 cell/mm2), lower hexagonality (P value 0.006) compared to control (48.25±18.76 vs 55.06±12,44), also a significantly higher coefficient of variation (p value 0.046) compared to control (36.15±7.381 vs 33.4±6.22). Conclusion: Pseudoexfoliation syndrome is associated with a significant change in the corneal endothelium, including reduction of endothelial cell density, increased variation of cells shape (pleomorphism) and increased variation of cells area (polymegathism). There was a significant change of central corneal thickness. Keywords: Corneal endothelium, specular microscopy, pseudoexfoliation syndrome, central corneal thickness Citation: Rasheed AM, Kadum DJ. Corneal changes in a sample of pseudoexfoliation Iraqi patients. Iraqi JMS. 2018; 16(3): 232-238. doi: 10.22578/IJMS.16.3.2
24
Vongsilapavattana, Varun, Wallop Iemsomboon, and Raveewan Chootanom. "Corneal Endothelial Cell Changes in The Eyes of Primary Angle Closure Suspects Treated Using Laser Peripheral Iridotomy Compared with Untreated Fellow Eyes at Phrarongkutklao Hospital." Journal of Southeast Asian Medical Research 2, no. 2 (2018): 98–102. http://dx.doi.org/10.55374/jseamed.v2i2.15.
Full textAbstract:
Objective: The study’s primary outcome was to compare corneal endothelial cell in the eyes of Primary Angle Closure Suspects (PACS) treated by laser peripheral iridotomy (LPI) and in untreated fellow eyes. The secondary outcome was to assess short-term effects of LPI on the corneal endothelium among PACS. Study design: The study employed a prospective design MethodsL PACS visiting Phramongkutklao Hospital were enrolled in the study. Data were collcted regarding type and setting of laser parameters, endothelial cell counts and morphology, gonioscopy and underlying diseases. Randomized eyes were treated using LPI while the other eye was treated by LPI 3 months later. Rate of corneal endothelium loss in the treated eyes were assessed and compared with untreated fellow eyes. Primary outcome was interpreted by pair t-test while secondary outcome was interpreted using the ANOVA test. Results: A total of 31 PACS (62 eyes) were included in the study. The medium age was 67 years (range 43-90). PACS totaled 21 females (67.7%) and 10 males (32.3%). The mean total power of double frequency Nd:YAG (532 nm) laser and Nd:YAG laser, were 855.6+-53.9 mW and 2.3+-0.5 mJ, respectively. Concerning primary outcomes, the mean coeneal endothelial cell density before LPI and postLPI 3 months was 2608.5+-399.8 and 2605.6+-397, respectively, 1 cellmm2. In untreated fellow eyes, the mean corneal endothelial cell density at 1 and 3 months was 2607.1+-419.6 and 2605.0+-403.2 cell/mm2, respectively. No significance was found in rate of endothelial cell change between treated and untreated fellow eyes using LPI (p=0.981). Regarding secondary outcomes, corneal endothelial cell density did not decrease significantly in 3 months (p=0.126) Conclusion: No difference was observed in corneal endothelial cell changes between treated and untreated fellow eyes using LPI over 3 months. LPI did nit affect corneal endothelial cell in a short term period
25
Chen, Conan Y., Steven J. Solar, Daniel S. Lewis, et al. "Canine Descemet Stripping Endothelial Keratoplasty with a Tissue Insertion Device: Technique and Long-Term Outcome." Case Reports in Veterinary Medicine 2023 (December 21, 2023): 1–5. http://dx.doi.org/10.1155/2023/7497643.
Full textAbstract:
Introduction. We describe a case of canine Descemet’s stripping endothelial keratoplasty (DSEK) using an open-source canine tissue delivery device. Case Presentation. We follow the four-year outcomes of a 1.5-year-old Tibetan Terrier who presented with difficulty seeing, diffuse corneal edema, and central corneal thickness of 1400 microns in the left eye. To perform DSEK, a polycarbonate carrier and insertion device was designed for canine corneas that measure up to 15 mm in diameter. The tissue was loaded into the inserter prior to surgery with the endothelium facing inwards and the stroma facing the cartridge wall. From the cartridge, the graft was pulled into the eye using microforceps and an anterior chamber maintainer. We assessed preoperative endothelial cell count, postoperative corneal clearance, and graft adhesion. The donor was a two-year-old Airedale Terrier who died one day prior to surgery, with endothelial cell density of 3149 cells/mm2. One week after DSEK, the cornea began to clear, and pachymetry of the donor and graft total was 1410 microns. This improved to 800 microns at 4 months and continued improving in its clarity at the last postoperative visit 4 years after surgery. Discussion. We demonstrate the feasibility of conducting canine endothelial keratoplasty with a specially designed tissue delivery device and the potential of long-term corneal clearance after DSEK in canine eyes.
26
Рыков, С. А., М. Г. Лысенко, И. В. Шаргородская, et al. "Endothelial Cells Survival in Human Corneal Grafts When Using the Modified Method Treatment of Keratoconus." Офтальмология. Восточная Европа, no. 3 (November 27, 2020): 311–27. http://dx.doi.org/10.34883/pi.2020.10.3.019.
Full textAbstract:
Проведена лазерная сканирующая конфокальная микроскопия донорских трупных роговиц, предназначенных для кератопластики. С помощью программного обеспечения ImageJ проанализирована рефлективность эндотелия и введено понятие коэффициента рефлективности эндотелиальных клеток роговицы (КРЭКР). В ходе статистической обработки определены морфометрические свойства эндотелиоцитов при конфокальной микроскопии, которые существенно влияют на потерю эндотелиальных клеток на трансплантате и, возможно, являются признаками апоптоза эндотелиоцитов. Разработана методика вископротекции эндотелия кадаверной роговицы на этапе формирования трансплантата. Отмечено, что сочетание качественной отбраковки донорского роговичного материала по разработанным критериям с проведением интраоперационной вископротекции эндотелия сокращает процент потери эндотелиальных клеток на роговичном трансплантате почти в 4 раза в течение первого года после трансплантации, что является профилактикой развития позднего эндотелиального отторжения роговичного трансплантата. Установлено, что общий (абсолютный и относительный) брак донорских роговиц для проведения сквозной аллокератопластики при кератоконусе составляет приблизительно 60%. Keratoconus is a disorder of the eye characterized by thinning and protrusion of the cornea, resulting in an irregular, conical shape and is the cause of persistent decline in vision and disability of young able-bodied people. Despite development of lamellar techniques of corneal surgery, penetrating keratoplasty remains the "gold standard" in the keratoconus treatment. The rapid loss of endothelial cells on the corneal graft at 6 months after keratoplasty is an important early symptom of late graft rejection (Lass J.H. et all, 2010). The aim of this study is to improve the quality of preservation ofendothelial cells on the native corneal grafts. This task can be solved by qualitative preliminary selection of donor tissue and reduction of intraoperative traumatization of endothelium on corneal graft.Methods. In Kyiv ophthalmic city hospital "Eye microsurgery center" (Kyiv, Ukraine) determined preoperative endothelium cells density by Heidelberg Retina Tomograph HRT II Rostock Cornea Module and were archived available endothelium cells images (590 human cadaveric corneas). Endothelial cells reflectivity analyses performed by ImageJ free software. Proposed to introduce the concept of the coefficient of reflectivity of corneal endothelial cells. Also noted the presence of organelles and "swelling" endothelial cells.In 1st clinic group (n=57) all the patients got standard penetrating keratoplasty procedure with standardly graft exams. Investigated the dependence of rapid endothelial cells loss with endothelial morphometric properties. In 2nd clinic group (n=29) all the patients got standard penetrating keratoplasty procedure with qualitative screening of corneas with new morphometric criteria (coefficient of corneal endothelial reflectivity more, than 50; without "swelling" endothelial cells and without organelles in the endothelial cells). In 3rd clinic group (n=43) patients got qualitative screening of corneas (like 2nd group), but viscoprotection during "back table" procedure has been developed.Results. As a result, combination of qualitative rejection of cadaveric corneas with morphometric endothelial factors reduces the percentage of cell loss four times. Tree morphometric factors in any combination were related with rapid loss of endothelial cells in graft: coefficient of corneal endothelial reflectivity lower, than 50; "swelling" endothelial cells and organelles in the endothelial cells. Determined that total (absolute and relative) qualitative rejection make unsuitable for penetrating keratoplasty for keratoconus approximately 60% of cadaveric corneas.Discussion. This study may help to reduce endothelial cells loss and frequency of late endothelial rejection of corneal grafts. Tree morphometric factors in any combination were related with rapid loss of endothelial cells in graft: coefficient of corneal endothelial reflectivity lower, than 50; "swelling" endothelial cells and organelles in the endothelial cells. Perhaps, these factors are markers of endothelial cells apoptosis.Conclusion. Investigated the dependence of rapid endothelial cells loss with endothelial morphometric properties. Proposed to introduce the concept of the coefficient of reflectivity of corneal endothelial cells. Proposed the methodology of endothelial viscoprotection during "back table" procedure before penetrating keratoplasty.
27
Dina, Maria-Silvia, Maria-Cristina Marinescu, Cătălina-Gabriela Corbu, Mihaela-Monica Constantin, Cătălina-Ioana Tătaru, and Călin-Petru Tătaru. "Impact of Corneal Crosslinking on Endothelial and Biomechanical Parameters in Keratoconus." Journal of Clinical Medicine 14, no. 13 (2025): 4489. https://doi.org/10.3390/jcm14134489.
Full textAbstract:
Background/Objectives: Keratoconus (KC) is a corneal ectatic disease, characterized by the progressive thinning of the cornea, myopia, and astigmatism, which lead to a decrease in visual acuity. Corneal collagen crosslinking (CXL) is an efficient method of stopping the progression of the disease. The objective of this study is to investigate the endothelial and biomechanical properties of the cornea in keratoconus patients, before and after undergoing corneal collagen crosslinking. Methods: A total of 66 eyes were diagnosed with progressive keratoconus and were recommended epi-off corneal crosslinking. Before the procedure, they were investigated with corneal topography (for minimum, maximum, average keratometry, and corneal astigmatism), specular microscopy (for the following endothelial cell parameters: number, density, surface, variability, and hexagonality), and an ocular response analyzer (for the following biomechanical parameters: corneal hysteresis and resistance factor). All measurements were repeated 1 month and 6 months after the intervention. Results: Several parameters differ according to the Amsler–Krumeich stage of keratoconus: in more advanced stages, patients present higher endothelial cell variability, a lower number of endothelial cells in the paracentral region of the cornea, lower CCT and CRF, and higher keratometry and astigmatism. Endothelial cell variability and number correlate with average keratometry, and there are also strong correlations between topography and CH and CRF. After CXL, the paracentral number of endothelial cells decreased; cell variability and average cell surface increased. Conclusions: More advanced keratoconus cases present with altered corneal biomechanics and topographical parameters, the endothelial layer also being affected proportional to the stage of the disease and also slightly affected after corneal collagen crosslinking.
28
Jiang, Guojian, and Tingjun Fan. "Sodium Ferulate Attenuates Lidocaine-Induced Corneal Endothelial Impairment." Oxidative Medicine and Cellular Longevity 2018 (July 8, 2018): 1–8. http://dx.doi.org/10.1155/2018/4967318.
Full textAbstract:
The introduction of intracameral anaesthesia by injection of lidocaine has become popular in cataract surgery for its inherent potency, rapid onset, tissue penetration, and efficiency. However, intracameral lidocaine causes corneal thickening, opacification, and corneal endothelial cell loss. Herein, we investigated the effects of lidocaine combined with sodium ferulate, an antioxidant with antiapoptotic and anti-inflammatory properties, on lidocaine-induced damage of corneal endothelia with in vitro experiment of morphological changes and cell viability of cultured human corneal endothelial cells and in vivo investigation of corneal endothelial cell density and central corneal thickness of cat eyes. Our finding indicates that sodium ferulate from 25 to 200 mg/L significantly reduced 2 g/L lidocaine-induced toxicity to human corneal endothelial cells, and 50 mg/L sodium ferulate recovered the damaged human corneal endothelial cells to normal growth status. Furthermore, 100 mg/L sodium ferulate significantly inhibited lidocaine-induced corneal endothelial cell loss and corneal thickening in cat eyes. In conclusion, sodium ferulate protects human corneal endothelial cells from lidocaine-induced cytotoxicity and attenuates corneal endothelial cell loss and central corneal thickening of cat eyes after intracameral injection with lidocaine. It is likely that the antioxidant effect of sodium ferulate reduces the cytotoxic and inflammatory corneal reaction during intracameral anaesthesia.
29
Abdellah, Marwa Mahmoud, Hatem Gamal Ammar, Mohamed Anbar, et al. "Corneal Endothelial Cell Density and Morphology in Healthy Egyptian Eyes." Journal of Ophthalmology 2019 (February 24, 2019): 1–8. http://dx.doi.org/10.1155/2019/6370241.
Full textAbstract:
Purpose. To evaluate the corneal endothelial cell density and morphology in normal Egyptian eyes. Methods. In total, 568 healthy eyes of 568 Egyptian volunteers aged 20 to 85 years were examined using noncontact specular microscopy for the central corneal thickness (CCT), mean endothelial cell density (MCD), coefficient of variation (CV) in cell area, mean cell area (MCA), and hexagonal cell (Hex) percentage. Variables were compared between sexes and between different age groups. Results. The mean CCT, MCD, and MCA were 514.45 ± 43.04 μm, 2647.50 ± 382.62 cells/mm2, and 390.59 ± 149.94 μm2, respectively. MCD and MCA showed no significant differences between men and women (P=0.171 and 0.099, respectively), whereas CV (%) and Hex (%) showed significant differences (P=0.024 and 0.015, respectively). CCT (P=0.007, r = −0.113) and MCD (P<0.001, r = −0.357) exhibited a significant negative correlation with age, whereas CV (%) (P<0.001, r = 0.341) and MCA (P=0.008, r = 0.111) exhibited a significant positive correlation. The mean rate of endothelial cell loss from 20 to 85 years of age was 0.3% per year. Conclusions. Our results provide normative data for the corneal endothelium in healthy Egyptian eyes, thus increasing the knowledge base for corneal endothelial cell parameters in healthy Egyptian eyes. Furthermore, our findings can be used as baseline values for comparisons between Egyptian and other populations and for studies of the endothelial cell reserve and capacity for intraocular surgery and corneal transplantation.
30
Wongvisavavit, Rintra, Mohit Parekh, Sajjad Ahmad, and Julie T. Daniels. "Challenges in corneal endothelial cell culture." Regenerative Medicine 16, no. 9 (2021): 871–91. http://dx.doi.org/10.2217/rme-2020-0202.
Full textAbstract:
Corneal endothelial cells (CECs) facilitate the function of maintaining the transparency of the cornea. Damage or dysfunction of CECs can lead to blindness, and the primary treatment is corneal transplantation. However, the shortage of cornea donors is a significant problem worldwide. Thus, cultured CEC therapy has been proposed and found to be a promising approach to overcome the lack of tissue supply. Unfortunately, CECs in humans rarely proliferate in vivo and, therefore, can be extremely challenging to culture in vitro. Several promising cell isolation and culture techniques have been proposed. Multiple factors affecting the success of cell expansion including donor characteristics, preservation and isolation methods, plating density, media preparation, transdifferentiation and biomarkers have been evaluated. However, there is no consensus on standard technique for CEC culture. This review aimed to determine the challenges and investigate potential options that would facilitate the standardization of CEC culture for research and therapeutic application.
31
Shaposhnikova, I. V., I. R. Gazizova, A. V. Kuroyedov, A. V. Seleznev, and D. N. Lovpache. "Loss of Corneal Endothelial Cells during Glaucoma Surgery." Ophthalmology in Russia 17, no. 4 (2020): 692–98. http://dx.doi.org/10.18008/1816-5095-2020-4-692-698.
Full textAbstract:
As known, there are changes in the biomechanical properties of the fibrous tunic in glaucoma. This is also due to the imbalance between the formation and loss of collagen fibers. The cornea is a highly sensitive tunic of the eyeball, endothelium is one of the target organs in the formation of pathological changes in primary open-angle glaucoma, and endothelial dysfunction, according to many authors, plays a significant role in its pathogenesis. The unintended consequences of glaucoma surgery lead to a progressive loss of corneal endothelial cells, which can lead to corneal decompensation. In the proposed literature review, we analyzed more than 30 publications by authors representing the results of multicenter studies of the pathological effects of antiglaucoma operations on the corneal endothelium in patients with various types of uncompensated glaucoma. The analysis of both classic fistulizing type operations and modern, so-called micro-invasive techniques (MIGS), including the use of various drains and devices, is presented. Thus, the smallest decrease in the density of endothelial cells was noted during deep sclerectomy and trabeculectomy. The use of mitomycin C in glaucoma surgery significantly improving the prognosis, but increases the loss of endothelial cells in both high and low concentrations. The highest rates of loss of corneal endothelial cells are noted during using valves and shunts. Ex-PRESS shunt implantation is associated with a significant loss of corneal endothelial cell density in the immediate area to the drainage tube, and therefore, shunt implantation should not be considered as an option for eyes with corneal dysfunction. In addition, the authors prove that the drainage device changes its position in the anterior chamber, especially when performing massage and self-massage of filtration bleb, which leads to a greater loss of density of corneal endothelial cells.
32
Opala, Aleksandra, Łukasz Kołodziejski, and Iwona Grabska-Liberek. "Impact of Well-Controlled Type 2 Diabetes on Corneal Endothelium Following Cataract Surgery: A Prospective Longitudinal Analysis." Journal of Clinical Medicine 14, no. 10 (2025): 3603. https://doi.org/10.3390/jcm14103603.
Full textAbstract:
Background: The aim of this study was to evaluate corneal endothelial changes following phacoemulsification cataract surgery with intraocular lens implantation in patients with type 2 diabetes (study group) and without diabetes (control group). The study aimed to determine the extent of endothelial cell damage and the regenerative capacity of the cornea in patients with well-controlled diabetes. Methods: This study compared corneal endothelial parameters in 80 eyes (80 patients) with well-controlled type 2 diabetes and 80 eyes (80 patients) without diabetes, all of whom underwent uneventful phacoemulsification cataract surgery. Patients were examined preoperatively and at 14 days, 3 months, and 6–8 months postoperatively. Endothelial cell density (ECD), percentage of hexagonal cells (%HEX), cell size variability (CV), and central corneal thickness (CCT) were assessed using a specular microscope. Visual acuity, intraocular pressure (IOP), and cumulative dissipated energy (CDE) during phacoemulsification were also measured. Results: The study and control groups were matched for age and sex. Preoperatively, patients with type 2 diabetes had significantly lower endothelial cell density (2480.76 ± 303.48 cells/mm2) compared to the control group (2629.64 ± 304.73 cells/mm2, p = 0.002). Visual acuity was also significantly lower in the study group (0.44 ± 0.18) than in the control group (0.50 ± 0.19, p = 0.049). No significant preoperative differences were observed in IOP, CV, %HEX, or CCT. Postoperatively, both groups experienced ECD decline: −18.44%, −18.77%, and −19.05% in the study group and −15.12%, −16.42%, and −16.73% in the control group at 14 days, 3 months, and 6–8 months, respectively. Differences between groups were not statistically significant (p = 0.285). A significant %HEX decrease was observed in both groups at all time points, with a greater decline in the study group at 14 days and 3 months. CV significantly increased in both groups at 14 days and 3 months postoperatively, but no significant difference was found between groups. A significant increase in CCT was observed at 14 days and 3 months postoperatively, with a greater increase in the study group at 14 days. Preoperative visual acuity negatively correlated with CDE in both groups. Additionally, CDE negatively correlated with ECD at all time points. Conclusions: Endothelial cell density is lower in patients with well-controlled type 2 diabetes than in non-diabetic individuals. Both groups are at risk of endothelial cell loss during phacoemulsification. Despite good glycemic control and comparable preoperative endothelial morphology, the cornea in diabetic patients is more vulnerable to damage, with a prolonged regeneration process. The impaired regenerative capacity of the corneal endothelium suggests the need for additional precautions during cataract surgery in diabetic patients. Despite ECD decline and delayed endothelial regeneration, the functional status of the cornea, as indicated by visual acuity and CCT, remains stable. The adequate corneal endothelial cell reserve in well-controlled type 2 diabetes patients allows for cataract surgery without significant corneal complications.
33
Ivanovska Adjievska, Biljana, and Violeta Buckoska. "CORNEAL EDEMA AFTER CATARACT SURGERY - CHANGES IN CORNEAL ENDOTHELIUM CELL CHARACTERISTICS." MEDIS – International Journal of Medical Sciences and Research 2, no. 2 (2023): 37–40. http://dx.doi.org/10.35120/medisij020237i.
Full textAbstract:

 Corneal edema is a common complication of cataract surgery, although tremendous improvements have been made in the surgical techniques, which decreased surgical eye trauma and complication rates. Normal endothelial cell density is 2000-3000 cells/mm2 in older individuals, which maintains the corneal clarity. Even ‘perfect’ cataract surgery does some damage to the endothelium. A significant postoperative endothelium density decrease can impair its ability to maintain corneal clarity, resulting in corneal edema, blurring of vision and ocular pain. Aggressive topical treatment in the first month after surgery may lead to recovery of the endothelial cells. Our aim was to establish the effects of cataract surgery on the characteristics of the corneal endothelium. We performed a prospective interventional clinical study of 30 patients, mean age 65±12 years, with senile cataract. Over 80% were hard cataracts: 18% hypermature, 66% grade 4 (brunescent) and 16% grade 3 cataracts. Uneventful phacoemulsification with IOL implantation was performed by one experienced phaco-surgeon in an outpatient setting. Preoperative parameters included: best-corrected visual acuity (BCVA) in Snellen decimal units, IOP, cataract density (slit lamp examination), corneal endothelium cell density (ECD) and hexagonality measured with a specular microscope. Intraoperative parameters included: phacoemulsification time and energy, irrigation–aspiration suction time. Standard phacoemulsification cataract surgery was performed with in-the-bag IOL implantation. Mean baseline parameters were: BCVA=0.1±0.13, IOP=15.7±2.7 mmHg, ECD=2,497±290 cells/mm2, cell hexagonality was 54.3±9.4%. Mean surgical parameters were: surgical time=9,3±2.9 minutes, phacoemulsification time=35.6±26.1 seconds, phacoemulsification energy=13.3±10.9J, irrigation–aspiration suction time=81.3±45.9 seconds. Acute postoperative corneal edema occurred in 4 eyes (13.3%). After one-week BCVA was 0.5±0.2. 9 eyes (30%) had visual acuity ≤0.5. They were treated aggressively with antibiotics (moxifloxacine), corticosteroid (dexamethasone) and hypertonic eye drops (sodium chloride (5%) and mannitol (20%)), every hour during the first week and gradually tapered in the 1 month. Antiglaucomatose eyedrops (timolol, brinzolamide) were used to control the IOP below 20 mmHg. After 1 month mean BCVA increased to 0.85±0.15 and all eyes reached BCVA higher than 0.6. IOP was stable at 15.4±2.0 mmHg. The mean endothelial cell loss was 19,1%. None of the eyes progressed to chronic edema. Corneal edema is a common complication after surgery of difficult cataracts. Even though the cataract density directly influences the postoperative condition of the corneal endothelium, surgical trauma is still considered the most common cause of corneal endothelial decompensation. Preoperative specular microscopy is very important to predict possible postoperative complications of the corneal endothelium and apply appropriate surgical techniques and materials. Modern phaco-techniques (low phaco-energy, small incision site, new irrigation solutions and OVDs) can significantly reduce endothelial cell loss after cataract surgery. It is recommended to treat postoperative corneal edema and inflammation with topical corticosteroids, topical hypertonic agents and to maintain intraocular pressure below 20 mmHg.
34
Wang, Xuemei, Yanlin Zhong, Minghui Liang, Zhirong Lin, Huping Wu, and Cheng Li. "Crosslinking-Induced Corneal Endothelium Dysfunction and Its Protection by Topical Ripasudil Treatment." Disease Markers 2022 (January 13, 2022): 1–12. http://dx.doi.org/10.1155/2022/5179247.
Full textAbstract:
Purpose. To investigate the changes of corneal endothelium under different crosslinking conditions and the protective effect of ripasudil. Methods. Corneal crosslinking groups were infiltrated with riboflavin and subsequently irradiated with 0.54 J/cm2 or 1.08 J/cm2 UVA, while noncrosslinking groups included neither UVA nor riboflavin treatment, only 1.08 J/cm2 UVA and only riboflavin treatment. Corneal opacity, variations in corneal endothelial cells, and corneal thickness of all groups were observed by slit lamp, in vivo confocal microscopy, and optical coherence tomography. Immunofluorescence staining and scanning electron microscopy were performed to evaluate changes in the structure and function of the corneal endothelium. The mice that received a corneal crosslinking dose of 1.08 J/cm2 were instilled with ripasudil to explore its protective effect on the corneal endothelium. Results. Treatment with UVA and riboflavin caused an increase in corneal opacity and corneal thickness and decreased endothelial cell density. Furthermore, treatment with UVA and riboflavin caused endothelial cell DNA damage and destroyed the tight junction and pump function of the endothelium, while riboflavin or the same dose of UVA alone did not affect the endothelium. Ripasudil reduced DNA damage in endothelial cells, increased the density of cells, and protected the endothelium’s integrity and function. Conclusion. Riboflavin combined with UVA can damage the corneal endothelium’s normal functioning. The corneal endothelium’s wound healing is dose-dependent, and the ROCK inhibitor ripasudil maintains the endothelium’s pump and barrier functions.
35
Smith, Christopher, Daniel Kaitis, Jordan Winegar, et al. "Comparison of endothelial/Descemet’s membrane complex thickness with endothelial cell density for the diagnosis of corneal transplant rejection." Therapeutic Advances in Ophthalmology 10 (January 2018): 251584141881418. http://dx.doi.org/10.1177/2515841418814187.
Full textAbstract:
Purpose: This study compared the effectiveness of endothelial/Descemet’s membrane complex thickness obtained using high-definition anterior segment optical coherence tomography with endothelial cell density obtained using confocal microscopy as diagnostic tools in predicting corneal transplant rejection. Methods: This observational, prospective, cross-sectional study evaluated penetrating keratoplasty grafts. Slit lamp examination organized the grafts into healthy or rejecting grafts. Grafts were scanned using both high-definition anterior segment optical coherence tomography and confocal microscopy. Central corneal thickness, endothelial/Descemet’s membrane complex thickness, endothelial cell density, and coefficient of variation were each compared with the clinical status. Descemet’s rejection index, defined by endothelial/Descemet’s membrane complex thickness divided by central corneal thickness multiplied by 33, further compared endothelial/Descemet’s membrane complex thickness with central corneal thickness. Results: Endothelial/Descemet’s membrane complex thickness, central corneal thickness, and Descemet’s rejection index were all able to differentiate between clear and rejected corneal grafts ( p < 0.0001, p = 0.001, and p = 0.012, respectively). Endothelial cell density and coefficient of variation did not correlate with the clinical status ( p = 0.054 and p = 0.102, respectively). Endothelial/Descemet’s membrane complex thickness had the largest area under the curve using receiver operating characteristic curves ( p < 0.0001). Endothelial/Descemet’s membrane complex thickness had a sensitivity of 86% and specificity of 81% with a cutoff value of >16.0 µm ( p < 0.0001). The sensitivity and specificity of endothelial cell density were both 71% with a cutoff value of ⩽897 cells/mm2 ( p = 0.053). There was a high correlation between endothelial/Descemet’s membrane complex thickness and both Descemet’s rejection index and central corneal thickness ( p < 0.0001). Conclusion: Endothelial/Descemet’s membrane complex thickness measured by high-definition anterior segment optical coherence tomography is a useful parameter for the diagnosis of corneal graft rejection. The diagnostic performance of endothelial/Descemet’s membrane complex thickness was significantly better than that of endothelial cell density and central corneal thickness. Endothelial cell density and the coefficient of variation were unable to diagnose corneal graft rejection in our cross-sectional study.
36
Javagal, Akshatha, Sandeep K, Kavitha Chikkanayakanahalli, Pavana Acharya, Sreelekshmi S R, and Narendra N. "Assessment on the evaluation of corneal endothelial cell morphology and cell count in cataract with pseudoexfoliation." Indian Journal of Clinical and Experimental Ophthalmology 9, no. 1 (2023): 92–96. http://dx.doi.org/10.18231/j.ijceo.2023.018.
Full textAbstract:
To assess the cell morphology & evaluate corneal endothelial cell count in patients suffering from cataract with pseudoexfoliation (PEX).A cross-sectional study was planned and conducted in 40 eyes of patients, corneal endothelial cell density (CD), central corneal thickness (CCT) and coefficient of variation (CV) & percentage of hexagonal cells (6A) in cell size were assessed with application of specular microscopeThe mean age of patients with cataract & PEX was 68.5 years. 58% were males. Lower CD was found in 40% patients of 50-59 years & 60% patients 60-69 years. Patients above 70 years had normal CD for age. 6A was low in 90% of PEX corneas. Abnormal CV was seen in 67.5% patients. CCT, CD & 6A were lower in PEX glaucoma compared to PEX. Our study confirms the existence of quantitative and qualitative abnormalities in corneal endothelial cells with PEX especially when IOP is high.
37
Bertolin, Marina, Alessandro Ruzza, Vanessa Barbaro, Elisa Zanetti, Diego Ponzin, and Stefano Ferrari. "Factors Affecting the Density of Corneal Endothelial Cells Cultured from Donor Corneas." International Journal of Molecular Sciences 25, no. 22 (2024): 11884. http://dx.doi.org/10.3390/ijms252211884.
Full textAbstract:
We investigated which specific correlation exists between the endothelial cell density (ECD) of corneal endothelial cell (CEC) cultures and the features of the donor corneas from which they originate. CEC cultures were prepared from one donor cornea or by pooling together cells of more corneas from elderly donors with ECDs lower or higher than 2000 cells/mm2. The ECDs of such primary cultures were evaluated and showed that that ECDs > 2000 cells/mm2 can be obtained only when CECs are isolated from (1) corneas of young donors; (2) at least two elderly donor corneas (if ECD > 2000 cells/mm2), or three elderly donor corneas (if ECD < 2000 cells/mm2). Secondary cultures are all characterized by ECDs < 2000 cells/mm2. Our study highlights the difficulties in obtaining cultures with ECDs > 2000 cells/mm2. Even if achievable with corneas from young donors, this becomes a challenging task when corneas from elderly donors are used (i.e., the overall majority of those collected by eye banks) and particularly when corneas from elderly donors with ECD < 2000 cells/mm2 are used. Pooling more of two corneas to obtain suitable CECs could technically overcome the problem. The above issues should be tackled appropriately before moving into clinical studies.
38
Gagnon, Marjolaine-Marie, H??l??ne M. Boisjoly, Isabelle Brunette, Manon Charest, and Marcel Amyot. "Corneal Endothelial Cell Density in Glaucoma." Cornea 16, no. 3 (1997): 314???318. http://dx.doi.org/10.1097/00003226-199705000-00010.
Full text
39
Rouhiainen, Hani, and Piia Kemppinen. "Corneal Endothelial Cell Density after Trabeculoplasty." Ophthalmologica 196, no. 4 (1988): 182–84. http://dx.doi.org/10.1159/000309897.
Full text
40
SETÄLÄ, KIRSI. "CORNEAL ENDOTHELIAL CELL DENSITY IN IRIDOCYCLITIS." Acta Ophthalmologica 57, no. 2 (2009): 277–86. http://dx.doi.org/10.1111/j.1755-3768.1979.tb00492.x.
Full text
41
Ostadian, Farshad, Fereydoun Farrahi, and Bahman Cheraghian. "Panretinal photocoagulation laser in diabetic retinopathy patients." Pakistan Journal of Medical and Health Sciences 15, no. 6 (2021): 1820–23. http://dx.doi.org/10.53350/pjmhs211561820.
Full textAbstract:
Background: It was reported that corneal endothelial cell depletion is a complication of the retinal laser. In this study, the effect of panretinal laser on corneal endothelium was investigated. Method: A group consisting of 47 eyes of 47 Diabetic patients who need a panretinal photocoagulation laser was compared with another group of 47 diabetic patients who did not require a laser. The patient's age was chosen in the range of 50-70 and the tools included Ellex double frequency LASER, Specular microscopy, Pachymetry, and Condense Lens 90. Time course of coefficient of variation, endothelial cell density, hexagonal cells and central corneal thickness were evaluated. ANOVA, Dennett's, and Mann-Whitney test was used to compare the data. P-value of <0.05 was considered statistically significant. Results: We found no significant differences in age, gender between the two groups. There was no significant differences in hexagonal cells, coefficient of variation, endothelial cell density and central corneal thickness between the two groups at any time points. Conclusion: This study suggests that the "panretinal photocoagulation laser" conserves corneal endothelial cells of subsequent damage in diabetic retinopathy patients Keywords: photocoagulation laser, corneal, endothelial cell, argon laser, double frequency laser.
42
Zhu, Ying-Ting, Fu Li, Bo Han, et al. "Activation of RhoA-ROCK-BMP signaling reprograms adult human corneal endothelial cells." Journal of Cell Biology 206, no. 6 (2014): 799–811. http://dx.doi.org/10.1083/jcb.201404032.
Full textAbstract:
Currently there are limited treatment options for corneal blindness caused by dysfunctional corneal endothelial cells. The primary treatment involves transplantation of healthy donor human corneal endothelial cells, but a global shortage of donor corneas necessitates other options. Conventional tissue approaches for corneal endothelial cells are based on EDTA-trypsin treatment and run the risk of irreversible endothelial mesenchymal transition by activating canonical Wingless-related integration site (Wnt) and TGF-β signaling. Herein, we demonstrate an alternative strategy that avoids disruption of cell–cell junctions and instead activates Ras homologue gene family A (RhoA)–Rho-associated protein kinase (ROCK)–canonical bone morphogenic protein signaling to reprogram adult human corneal endothelial cells to neural crest–like progenitors via activation of the miR302b-Oct4-Sox2-Nanog network. This approach allowed us to engineer eight human corneal endothelial monolayers of transplantable size, with a normal density and phenotype from one corneoscleral rim. Given that a similar signal network also exists in the retinal pigment epithelium, this partial reprogramming approach may have widespread relevance and potential for treating degenerative diseases.
43
Pigatto, João Antonio Tadeu, Angela Aguiar Franzen, Fabiana Quartiero Pereira, et al. "Scanning electron microscopy of the corneal endothelium of ostrich." Ciência Rural 39, no. 3 (2009): 926–29. http://dx.doi.org/10.1590/s0103-84782009005000001.
Full textAbstract:
The aim of this study was to examine the endothelial surface morphology and perform a morphometric analysis of the corneal endothelial cells of ostrich (Struthio camelus) using scanning electron microscopy. Polygonality, mean cell area, cell density and coefficient of variation of mean cell area were analyzed. The normal corneal endothelium consisted of polygonal cells of uniform size and shape with few interdigitations of the cell borders. Microvilli appeared as protusions on the cellular surface. The average cell area was 269±18µm² and the endothelial cell density was 3717±240cells mm-2. The coefficient of variation of the cell area was 0.06, and the percentage of hexagonal cells was 75%. The parameters evaluated did not differ significantly between the right and the left eye from the same ostrich. The results of this study showed that the ostrich corneal endothelial cells appear quite similar to those of the other vertebrates.
44
Chaudhury, Pranati, Anita Misra, Niranjan Puthal, Sasmita Sahoo, Bichismita Jena, and Rajesh Panda. "Comparison of central corneal thickness and endothelial cell count among diabetic retinopathy and non-diabetic cases." Panacea Journal of Medical Sciences 13, no. 3 (2023): 631–34. http://dx.doi.org/10.18231/j.pjms.2023.116.
Full textAbstract:
: Diabetes is associated with alterations in the structure and functions of corneal endothelial cells. Current research has been carried out to compare the central corneal thickness and endothelial cell density in patients with diabetic retinopathy (DR) and non-diabetic (ND) individuals. : A Cross Sectional comparative research was carried out in a tertiary eye care hospital, over the period of two years from April 2021 to March 2023. Among 160 study participants, 80 cases with diabetic retinopathy (DR) and 80 non-diabetic (ND) cases as age matched control have been selected. Thorough Ophthalmic assessment was done. We have included only retinopathy cases as they have longer duration of diabetis and poorer metabolic control, for better comparison of the endothelium parameters with non-diabetics. Specular microscopy has been done in all cases for endothelial cell count evaluation and thickness of cornea has been estimated by Pachymeter. Statistical analysis was carried out by students‘t’ test by comparing the variables between two groups. : P-value was not significant for the mean age and sex distribution in the two groups. Mean endothelial cell density was lesser (2512.12±260.23cells/mm2) in DR than in ND group (2699.10±95.68cells/mm2). Mean central corneal thickness was greater (522.65±36.56μm) in DR than in ND group (486.50±18.67μm) (P&#60;0.05). Also, the Co-efficient of variation percentage was more whereas the percentage of hexagonality was found to be statistically less in DR than ND group. Among DR patients, endothelial cell density was significantly reduced and central corneal thickness considerably raised when compared with ND. Our results also suggest that poor metabolic control and advanced Diabetic Retinopathy are risk factors for developing keratopathy.
45
Chen, Xu, Yi Shao, Shi-Nan Wu, and Shan-Bi Zhou. "Potential Factors of Corneal Endothelial Cells for Progression in Children with Uveitis." Disease Markers 2021 (December 28, 2021): 1–10. http://dx.doi.org/10.1155/2021/8432774.
Full textAbstract:
Objective. To observe the morphological changes and abnormal structure of corneal endothelial cells in children with uveitis, to analyze the related factors affecting the morphological changes of corneal endothelial cells, and to explore the clinical application of a corneal endothelial microscope in children with uveitis. Methods. The corneal endothelial cells of 70 patients with uveitis were photographed with the Topcon SP-3000 noncontact corneal endothelial microscope, and the corneal endothelial cell density (CD), average cell area (AVE), coefficient of variation of the cell area (CV), and percentage of hexagonal cells (PHC) were measured with the IMAGEnet system. Twenty-eight patients (56 eyes) with monocular uveitis were selected, with the affected eyes (28 eyes) as the experimental group and the contralateral healthy eyes (28 eyes) as the control group. The corneal endothelial cell parameters between the two groups were statistically analyzed. The parameters of corneal endothelial cells in 70 children with uveitis were compared, and the effects of the course of the disease, inflammatory cells in the anterior chamber, and posterior corneal deposition (KP) on the parameters of corneal endothelial cells were analyzed. Results. There are four abnormal forms of the corneal endothelium in children with uveitis: enlarged cell area gap, irregular cell shape, blurred intercellular space, and cell loss. KP showed irregular high reflective white spots in the corneal endothelial microscope images, surrounded by dark areas, and existed in all the eyes with dusty KP found in slit lamp examination and a small number of eyes without obvious KP. Comparing the corneal endothelial cell parameters between the experimental group and the control group, it was found that the corneal endothelial CD and PHC of the former were lower than those of the latter, and the difference was statistically significant ( P < 0.001 and P = 0.018 , respectively). The AVE and CA of the former were higher than those of the latter ( P = 0.013 and P = 0.046 , respectively). The corneal endothelial cell density of the eyes with a course of the disease of more than 1 year was lower than that of the eyes with a course of the disease less than 1 year, the coefficient of variation of the corneal endothelial cell area of the eyes with KP was higher than that of the eyes without KP, and the difference was statistically significant ( P = 0.003 and P = 0.030 , respectively). Conclusion. Corneal endothelial microscopy is one of the important methods for the detection of uveitis with high sensitivity. The change of morphological parameters of corneal endothelial cells is one of the important indexes to assist in the diagnosis of uveitis and can be further promoted in ophthalmological examination.
46
Kandemir, Baran, Nesrin Tutaş Günaydın, Eren Göktaş, and Burak Tanyıldız. "Does Storage Time Affect the Outcomes of Split Corneal Transplantation to Reduce Corneal Donor Shortage? A Retrospective Study." INQUIRY: The Journal of Health Care Organization, Provision, and Financing 58 (January 2021): 004695802110458. http://dx.doi.org/10.1177/00469580211045846.
Full textAbstract:
Split cornea transplantation can reduce the shortage of donor corneas. Therefore, this study aimed to evaluate the effect of split graft storage time on the outcomes of split corneal transplantation through Descemet membrane endothelial keratoplasty (DMEK) and deep anterior lamellar keratoplasty (DALK) surgeries. Split corneal transplantation was performed in 80 eyes using 41 donor corneas. The mean before and after splitting storage times and total storage times were recorded. Donor corneal buttons and split grafts were stored in short-term solution at 4°C. In both surgeries (DMEK and DALK), donor corneas were divided into groups depending on their storage times. Mean postoperative 12th month best corrected visual acuity (BCVA), endothelial cell density (ECD), endothelial cell loss (ECL), central corneal thickness (CCT), refractive spherical equivalent (RSE), refractive astigmatism, and complication rates were compared among the groups. Correlation between storage times and 1-year BCVA, ECL, and complication rates were assessed. Clinical outcomes of the groups 1 year after the surgeries were also compared. DALK and DMEK were performed in 41 and 39 eyes, respectively. Storage times were not correlated with 1-year DMEK outcomes and only weakly correlated with post-DALK ECD, ECL, and RSE values. Except for CCT in those that underwent DALK, the outcomes of DMEK and DALK surgeries with stored and non-stored split grafts were not significantly different ( P = .02). The storage times of donor corneas and split grafts do not have any impact on outcomes.
47
Papadakou, Panagiota, Irini Chatziralli, Miltiadis Papathanassiou, et al. "The Effect of Diabetes Mellitus on Corneal Endothelial Cells and Central Corneal Thickness: A Case-Control Study." Ophthalmic Research 63, no. 6 (2020): 550–54. http://dx.doi.org/10.1159/000507197.
Full textAbstract:
<b><i>Purpose:</i></b> The aim of this study was to evaluate the characteristics of corneal endothelial cells and central corneal thickness (CCT) in patients with diabetes mellitus (DM), comparing them with those of healthy subjects (controls) and to determine potential factors affecting the corneal parameters in patients with DM. <b><i>Methods:</i></b> Participants in this study were 72 patients with DM and 88 healthy controls. Diabetic patients were further classified into groups depending on the severity of diabetic retinopathy (no retinopathy, mild, moderate, severe non-proliferative diabetic retinopathy, and proliferative diabetic retinopathy). All participants underwent non-contact specular microscopy to evaluate corneal endothelium parameters and CCT, while factors affecting endothelial cell density and CCT in patients with DM were also analyzed. <b><i>Results:</i></b> Patients with DM presented significantly decreased endothelial cell density compared to controls (2,297.9 ± 311.3 and 2,518.3 ± 243.7 cells/mm<sup>2</sup>, respectively; <i>p</i> &#x3c; 0.001), while the two groups did not differ significantly in any other measured corneal parameter. In the diabetic group, the multivariate analysis showed a significant association between decreased endothelial cell density and increased HbA1c (<i>p</i> &#x3c; 0.001), longer DM duration (<i>p</i> = 0.003), and more severe diabetic retinopathy status (<i>p</i> = 0.008). <b><i>Conclusion:</i></b> DM seems to affect the corneal endothelium, since endothelial cell density was decreased in the diabetic group, while duration of disease, HbA1c levels, and severity of retinopathy were significantly associated with changes in endothelial cell density and should be taken into account.
48
Azdasheer Mofied Dalloul, Yusuf Mohammed Suleiman, Najwa Fay, Azdasheer Mofied Dalloul, Yusuf Mohammed Suleiman, Najwa Fay. "Studying the changes of corneal endothelium cells in patients with spherical refractive errors: دراسة التغيرات في بطانة القرنية عند مرضى أسواء الانكسار الكروية". Journal of medical and pharmaceutical sciences 6, № 1 (2022): 1–17. http://dx.doi.org/10.26389/ajsrp.d080821.
Full textAbstract:
Objective: To study of the relationship between spherical refractive errors and the changes in corneal endothelial cells. Methods: This sectional study, 1098 eyes of 663 persons attending the ophthalmological clinic at Tishreen University Hospital were studied between the years 2020-2021, they were divided into three groups according to the spherical equivalent value (193 nearsighted, 254 partially sighted, 216 long sighted), and also each was divided into three groups according to the value of the spherical equivalent. From the farsightedness and myopia groups to groups according to the severity of the refraction present (mild, moderate, severe), all participants underwent a complete ophthalmological examination including corneal endothelial cell counts by Endothelium Mic. Non contact, Specular Microscope, where the average of three successive measurements of endothelial cell density (MCD), coefficient of variation (CV) for cell volume, and hexogram (EX%) were taken. Results: The values of endothelial cell density (MCD) (P ˂ 0.05) and hexameric cell percentage (EX%) (P ˂ 0.05) decreased statistically significantly, while the value of the covariance (CV) increased (P ˂ 0.05) with increasing myopia, This change was at the expense of moderate myopia and severe myopia, while mild myopia did not have a statistically significant effect, as well as for hyperopia, where we did not find significant statistical differences in the three variables (MCD) (EX%) (CV) (P ˃ 0.05). Conclusion: Hyperopia does not affect the endothelial cells of the cornea, while moderate to severe myopia causes a significant change in the endothelial cells of the cornea.
49
Norhani, Mohidin, Yu Chen Low, Mohd-Ali Bariah, Mohamad Shahimin Mizhanim, and Arif Norlaili. "Corneal endothelial morphology of healthy myopic Malaysian children of Chinese ethnicity aged 8-9 years and its association with axial length." F1000Research 11 (September 7, 2022): 339. http://dx.doi.org/10.12688/f1000research.110560.2.
Full textAbstract:
Background: This is a cross-sectional study to further understand the effects of axial length elongation on the corneal endothelial cell’s morphology in myopic children. Abnormal changes in the morphology of corneal endothelium are indicators of corneal stress or instability which could be linked to myopia. Methods: 111 school children comprising of 37 emmetropes, 37 mild myopes and 37 moderate myopes aged 8-9 years old were recruited. Visual acuity was measured using the LogMar chart, cycloplegic refraction was determined using an open-field autorefractor (Grand Seiko WAM-5100, Hiroshima, Japan) and refined using subjective refraction. Morphology of corneal endothelial cells [endothelial cell density, coefficient of variation, hexagonality and central corneal thickness] was evaluated using a non-contact specular microscope (Topcon SP-2000P). Axial length was measured with A-scan ultrasound biometry (PacScan Plus, Sonomed Escalon, NY). The correlation between morphology of corneal endothelial cells and axial length were assessed using Pearson Correlation and Linear regression analysis. Results: There was no significant difference in corneal endothelial cells and axial length between gender (p>0.05). Significant reduction in endothelial cells density and hexagonality and increased coefficient of variation was found in eyes of higher myopic power which had longer axial when compared to emmetropes (p<0.001). Except for central corneal thickness, all corneal endothelial cells parameters correlated significantly with axial length (p<0.05). For every 1mm increase in axial length, endothelial cells density decreased by 73.27cells/mm2, hexagonality decreased by 2.32% and coefficient of variation increased by 1.75%. Conclusions: There were significant changes in morphology of cornea endothelial cells in young moderate myopic children of Chinese ethnicity at 8-9 years of age. This result provides normative data for Malaysian children of Chinese ethnicity that can be used for comparison and reference for clinical procedures, thereby facilitating decision-making with respect to interventions for myopia control, especially in prescribing contact lens for children.
50
Norhani, Mohidin, Yu Chen Low, Mohd-Ali Bariah, Mohamad Shahimin Mizhanim, and Arif Norlaili. "Corneal endothelial morphology of healthy myopic Malaysian children of Chinese ethnicity aged 8-9 years and its association with axial length." F1000Research 11 (March 21, 2022): 339. http://dx.doi.org/10.12688/f1000research.110560.1.
Full textAbstract:
Background: This is a cross-sectional study to further understand the effects of axial length elongation on the corneal endothelial cell’s morphology in myopic children. Abnormal changes in the morphology of corneal endothelium are indicators of corneal stress or instability which could be linked to myopia. Methods: 111 school children comprising of 37 emmetropes, 37 mild myopes and 37 moderate myopes aged 8-9 years old were recruited. Visual acuity was measured using the LogMar chart, cycloplegic refraction was determined using an open-field autorefractor (Grand Seiko WAM-5100, Hiroshima, Japan) and refined using subjective refraction. Morphology of corneal endothelial cells [endothelial cell density, coefficient of variation, hexagonality and central corneal thickness] was evaluated using a non-contact specular microscope (Topcon SP-2000P). Axial length was measured with A-scan ultrasound biometry (PacScan Plus, Sonomed Escalon, NY). The correlation between morphology of corneal endothelial cells and axial length were assessed using Pearson Correlation and Linear regression analysis. Results: There was no significant difference in corneal endothelial cells and axial length between gender (p>0.05). Significant reduction in endothelial cells density and hexagonality and increased coefficient of variation was found in eyes of higher myopic power which had longer axial when compared to emmetropes (p<0.001). Except for central corneal thickness, all corneal endothelial cells parameters correlated significantly with axial length (p<0.05). For every 1mm increase in axial length, endothelial cells density decreased by 73.27cells/mm2, hexagonality decreased by 2.32% and coefficient of variation increased by 1.75%. Conclusions: There were significant changes in morphology of cornea endothelial cells in young moderate myopic children of Chinese ethnicity at 8-9 years of age. This result provides normative data for Malaysian children of Chinese ethnicity that can be used for comparison and reference for clinical procedures, thereby facilitating decision-making with respect to interventions for myopia control, especially in prescribing contact lens for children.