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Relevant bibliographies by topics / Cytofluorimetrie

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Academic literature on the topic 'Cytofluorimetrie'

Author: Grafiati

Published: 4 June 2021

Last updated: 31 January 2023

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Journal articles on the topic "Cytofluorimetrie"

1

Kit, O. I., N. K. Guskova, O. N. Selyutina, V. V. Dmitrieva, I. A. Novikova, I. S. Torpujyan, and V. R. Zakharchenko. "FEATURES OF A DIFFERENTIAL DIAGNOSIS OF PEDIATRIC ACUTE MONOCYTIC LEUKEMIA (AML-M5a) ON THE EXAMPLE OF A CLINICAL CASE." South Russian Journal of Cancer 1, no. 1 (March 7, 2020): 76–83. http://dx.doi.org/10.37748/2687-0533-2020-1-1-7.

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The aim of this work was to assess the significance of investigating clinical and laboratory parameters for diagnosing acute monocytic leukemia in children on the basis of a clinical case. The article demonstrates specific features of differentiating AML M5a from other forms of acute myeloid leukemia. According to the results of hematological, morphological and cytofluorimetric studies of blood and bone marrow samples, the diagnosis of acute myeloid leukemia was established. The morphological and phenotypic characteristics of blast cells hampered the diagnosis of an AML form. However, a comprehensive analysis of the expressed CD antigens allowed acute monocytic leukemia to be identified, which diagnosis was subsequently confirmed by a cytochemical study. Thus, the clinical diagnosis was established over a short period of time. This was of importance given the rising severity of the patient’s condition requiring immediate treatment, the initial hyperleukocytosis and the development of life-threatening complications associated with leukostasis in the lungs and the central nervous system. In the presented case, the clinical manifestations of the underlying disease and the results of flow cytofluorimetry were determining factors in initiating timely specific therapy.

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Gesi, Marco, Daniela Galli, Prisco Mirandola, Cecilia Carubbi, Elena Masselli, Marco Vitale, and Giuliana Gobbi. "Cytofluorimetric Platelet Analysis." Seminars in Thrombosis and Hemostasis 40, no. 01 (December 31, 2013): 088–98. http://dx.doi.org/10.1055/s-0033-1363472.

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De Figueroa, Jose Manuel Tierno, Francesco Buonocore, Massimo Mazzini, and Giuseppe Scapigliati. "Cytofluorimetric analysis ofBacillus rossiushaemocytes (Phasmatodea, Bacillidae)." Italian Journal of Zoology 68, no. 1 (January 2001): 9–14. http://dx.doi.org/10.1080/11250000109356377.

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Brickman, M. J., J. M. Cook, and A. E. Balber. "Low temperature reversibly inhibits transport from tubular endosomes to a perinuclear, acidic compartment in African trypanosomes." Journal of Cell Science 108, no. 11 (November 1, 1995): 3611–21. http://dx.doi.org/10.1242/jcs.108.11.3611.

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We have used electron microscopy and flow cytofluorimetry to study endocytosis and intracellular transport of fluid phase bovine serum albumen gold complexes and membrane bound concanavalin A through endosomal compartments of bloodstream forms of Trypanosoma brucei rhodesiense. Both markers were rapidly endocytosed from the flagellar pocket. Within 20 minutes at 37 degrees C the markers reached a large, vesicular, perinuclear compartment that stained heavily with the CB1 monoclonal antibody. Neither marker left the flagellar pocket and entered cells at 4 degrees C. When cells were incubated at 12 degrees C, both markers entered the cell and were transported to collecting tubules, a tubular endosomal compartment that receives endocytosed material from coated endocytic vesicles. However, no material was transported from collecting tubules to the late, perinuclear compartment at 12 degrees C. The morphology of collecting tubule membranes was specifically altered at 12 degrees C; tubules became shorter and were arrayed near the flagellar pocket. The morphological alteration and the block in transport of endocytic markers to the perinuclear compartment seen at 12 degrees C were reversed 10 minutes after cells were returned to 37 degrees C. We also used flow cytofluorimetric measurements of pH dependent fluorescence quenching to measure the pH of the terminal endocytic compartment. Fluoresceinated lectins accumulated in a terminal compartment with a pH of 6.0-6.1, a value considerably higher than that of mammalian lysosomes. Fluorescence from fluoresceinated lectins in this terminal endocytic compartment was dequenched when bloodstream forms were incubated in the presence of chloroquine.

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Testa, Antonia Carla, Francesco Pomini, Andrea Fattorossi, Alessandra Battaglia, Gabriella Ferrandina, Donata Mansueto, Carmen Mastromarino, Giovanni Scambia, and Alessandro Caruso. "Doppler Velocimetry and Cytofluorimetric Analysis inUterine Myomas." Gynecologic and Obstetric Investigation 56, no. 3 (2003): 139–42. http://dx.doi.org/10.1159/000073772.

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Dahlström, A., T. Kling-Petersen, S. Bööj, K. Lundmark, and P. A. Larsson. "Quantification of axonally transported material using cytofluorimetric scanning." Journal of Microscopy 155, no. 1 (July 1989): 61–80. http://dx.doi.org/10.1111/j.1365-2818.1989.tb04298.x.

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Sazanova, E. V., T. P. Shmelkova, A. L. Kravtsov, T. A. Malyukova, and Yu A. Popov. "FLOW-CYTOFLUORIMETRIC ANALYSIS OF CYTOTOXICITY OF YERSINIA PESTIS STRAINS." Journal of microbiology epidemiology immunobiology, no. 6 (December 28, 2017): 3–9. http://dx.doi.org/10.36233/0372-9311-2017-6-3-9.

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Braidotti, Stefania, Raffaella Franca, Marilena Granzotto, Elisa Piscianz, Alberto Tommasini, Marco Rabusin, Gabriele Stocco, and Giuliana Decorti. "Cytofluorimetric assay to investigate variability in blinatumomab in vitro response." Frontiers in Bioscience-Landmark 27, no. 2 (January 24, 2022): 039. http://dx.doi.org/10.31083/j.fbl2702039.

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Iannelli, D. "Cytofluorimetric Method for the Detection of the Cucumber Mosaic Virus." Phytopathology 86, no. 9 (1996): 959. http://dx.doi.org/10.1094/phyto-86-959.

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Khochbin, Saadi, Agnès Chabanas, Philippe Albert, and Jean-Jacques Lawrence. "Flow cytofluorimetric determination of protein distribution throughout the cell cycle." Cytometry 10, no. 4 (July 1989): 484–89. http://dx.doi.org/10.1002/cyto.990100418.

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Dissertations / Theses on the topic "Cytofluorimetrie"

1

Bouchaour, Ibrahim. "Etude de variants androgénétiques de Nicotiana plumbaginifolia Viv. Obtenus à partir de plantes haploides : Caractérisations, culture de protoplastes et régénération." Dijon, 1992. http://www.theses.fr/1992DIJOPE03.

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Sève, Annie-Pierre. "Lectines nucleaires." Orléans, 1987. http://www.theses.fr/1987ORLE2020.

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MORVAN, PIERRE-YVES. "Etude des facteurs qui conditionnent in vitro l'expression des genes de l'interleukine-2, de l'interferon-gamma, et de l'interleukine-4 dans les lymphocytes humains : par une methode d'analyse cytofluorimetrique adaptee a la detection des arn messagers et des proteines au niveau cellulaire." Rennes 1, 1996. http://www.theses.fr/1996REN10062.

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Nous avons etudie l'expression des genes de cytokines dans les lymphocytes humains, par deux methodes permettant d'avoir acces aux produits des genes au niveau cellulaire: les arnm par hybridation in situ (his) a l'aide de sondes non radioactives ; et les proteines reconnues par des anticorps en immunofluorescence (if). Ces deux methodes ont ete adaptees a l'analyse cytofluorimetrique, permettant de selectionner les cellules d'interet - les lymphocytes - au sein de suspensions cellulaires heterogenes. La technique d'his a ete mise au point dans les cellules de la lignee jurkat pour la detection des arnm de l'il-2 ; ce modele nous a permis de definir les conditions d'hybridation et de detection permettant d'obtenir un signal fluorescent specifique et reproductible. L'expression des arnm de l'il-2, de l'ifn- et de l'il-4 a ensuite ete etudiee apres activation in vitro dans des lymphocytes humains: des lymphocytes parmi les cellules mononucleees du sang peripherique (pbmc), des lymphocytes t purifies, des lymphocytes t cd4 et t cd8 isoles des pbmc et des clones t generes in vitro. Les frequences des cellules exprimant les arnm de l'il-2 et de l'ifn-, ainsi que les niveaux d'expression par cellule, estimes par l'intensite du signal de fluorescence, ont ete analyses en fonction de la nature de l'activateur, du temps d'activation, et du type de suspension cellulaire etudiee. Nous avons egalement mesure l'influence de l'environnement, des cellules accessoires et de l'apport de cytokines exogenes, ainsi que le role des signaux de costimulation delivres par des ligands solubles. Les resultats de l'etude de la production de ces memes cytokines - il-2, ifn- et il-4 - au niveau cellulaire, par une analyse en if, a l'aide d'anticorps reconnaissant la proteine cytoplasmique, montrent une bonne correlation entre les methodes et une simultaneite de la transcription et de la traduction. De plus, l'analyse en if permet l'identification des caracteristiques des cellules productrices en double marquage, par la mise en evidence de molecules de surface definissant des populations et des sous-populations cellulaires ou des stades d'activation. La capacite de production d'il-4 a ete etudiee dans des clones t, ainsi que les facteurs qui favorisent sa production dans les lymphocytes t humains isoles du sang. Nous avons montre une difference entre les lymphocytes t cd4 et t cd8 en terme de capacite de production des cytokines de type th1 (il-2 et ifn-) ou th2 (il-4). Le profil de differenciation des lymphocytes t etait conditionnes par des facteurs d'environnement, des interactions avec des cellules accessoires et des mediateurs solubles

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Loureiro, Luís Carlos Sá. "Functional characterization of mitochondrial suspensions by cytofluorimetric techniques towards the screening of mitochondrial target drugs." Master's thesis, 2015. http://hdl.handle.net/1822/35712.

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Dissertação de mestrado em Bioquímica Aplicada
Besides its role in respiration, mitochondria control other key metabolic pathways, cell proliferation and regulated cell death, with a crucial role in the regulation of the intrinsic apoptotic pathway. Recent studies have focused on the screen for anti-cancer agents that induce apoptosis through permeabilization of the mitochondrial outer membrane, leading to the cytosolic release of pro-apoptotic proteins and to the activation of the caspase cascade and consequently to cell dismantling. In the present study, staining protocols were developed and optimized aiming at the structural and functional characterization of isolated mitochondrial populations from yeast cells and rat liver by flow cytometry. Mitotracker Green and Nonyl Acridine Orange were used to measure changes in mitochondrial mass, 3,3'- Dihexyloxacarbocyanine Iodide and Mitotracker Red CMXROS to monitor the electrical potential of the inner membrane (Δψm), Mitotracker Red CM-H2XROS and Dihydroethidium to evaluate the accumulation of ROS, and 2',7'-Bis-(2-Carboxyethyl)- 5-(and-6)-Carboxyfluorescein and Acetoxymethyl Ester (BCECF-AM) to measure the pH of the mitochondrial matrix. Subsequent flow cytometry studies were designed to evaluate the effect in mitochondria function of two well-known apoptosis inducers in both mammalian cell lines and yeast cells, namely bovine lactoferrin (bLf) and acetate, and to understand whether or not they act directly on mitochondria independently of upstream cellular pathways. The results showed that both bLf and acetate promoted changes in mitochondrial mass, matrix mitochondrial pH, Δψm and ROS levels of rat liver mitochondria. Moreover, the assessment of the activity of the complexes of electron transport chain (ETC) showed that bLf and acetate affect their activity and trigger mitochondrial swelling. These results are particularly interesting since the two compounds appear to induce in isolated mitochondria mainly the same alterations as those occurring in whole cells, and which have been proposed to be involved in the induction of cell death. Altogether, this study show that flow cytometry is a valuable tool to study key mitochondrial functional parameters and to screen for mitochondriatargeted drugs, including the anticancer agents, such as acetate and bLf.
Para além do seu papel na respiração celular, as mitocôndrias controlam outros aspetos chaves do metabolismo, bem como a proliferação celular e a morte celular regulada, tendo um papel crucial na regulação da via apoptótica intrínseca. Estudos recentes têm-se focado na procura de agentes anticancerígenos capazes de induzir apoptose mediada pela permeabilização da membrana mitocondrial externa, conduzindo à libertação de proteínas pró-apoptóticas e à ativação da cascata de caspases e consequente desmantelamento celular. No presente estudo, foram desenvolvidos e otimizados protocolos de marcação com vista à caracterização estrutural e funcional por citometria de fluxo de populações de mitocôndrias isoladas de células de levedura e de hepatócitos de rato. As sondas Mitotracker Green e laranja de nonil-acridina foram usadas para medir variações da massa mitocondrial, o iodeto de 3,3'-Dihexiloxacarbocianina e o Mitotracker Red CMXROS para medir o potencial eléctrico da membrana mitocondrial interna (Δψm), o Mitotracker Red CM-H2XROS e dihidroetídeo para avaliar a acumulação de ROS e o acetoximetil éster de (2',7'-Bis-(2- Carboxietil)-5-(and-6)-carboxifluoresceína e(BCECF-AM) para medir o pH da matriz mitocondrial. Os estudos subsequentes de citometria de fluxo foram desenhados para avaliar o efeito de diferentes drogas na função mitocondrial, em particular da lactoferrina bovina (bLf) e do acetato, dois reconhecidos indutores de apoptose, no sentido de se compreender se atuam diretamente na mitocôndria independentemente de outras vias celulares a montante. Os resultados mostraram que tanto a bLf como o acetato promovem alterações na massa mitocondrial, pH da matriz mitocondrial, Δψm e nos níveis de ROS em mitocôndrias isoladas de fígado de rato. Além disso, a avaliação da atividade dos complexos da cadeia de transportadora de eletrões (ETC) mostrou que a bLf e o acetato afetam a sua atividade e desencadeiam a tumefação mitocondrial. Estes resultados são particularmente interessantes uma vez que os dois compostos parecem induzir, em mitocôndrias isoladas as mesmas alterações que ocorrem em células inteiras, e que têm sido propostas como estando envolvidas na indução da morte celular. No seu conjunto, este estudo mostra que a citometria de fluxo é uma ferramenta valiosa para o estudo da funcionalidade mitocondrial e para o rastreio de drogas que têm como alvo a mitocôndria, incluindo agentes anticancerígenos, tais como a bLf e o acetato.

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Books on the topic "Cytofluorimetrie"

1

Elli, Kohen, ed. Atlas of cell organelles fluorescence. Boca Raton: CRC Press, 2004.

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Hirschberg, Joseph G., Elli Kohen, and Ren Santus. Atlas of Cell Organelles Fluorescence. Taylor & Francis Group, 2004.

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Hirschberg, Joseph G., Rene Santus, Nuri Ozkutuk, and Elli Kohen. Atlas of Cell Organelles Fluorescence. Taylor & Francis Group, 2003.

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Hirschberg, Joseph G., Rene Santus, Nuri Ozkutuk, and Elli Kohen. Atlas of Cell Organelles Fluorescence. Taylor & Francis Group, 2003.

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Hirschberg, Joseph G., Rene Santus, Nuri Ozkutuk, and Elli Kohen. Atlas of Cell Organelles Fluorescence. Taylor & Francis Group, 2003.

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Hirschberg, Joseph G., Rene Santus, Nuri Ozkutuk, and Elli Kohen. Atlas of Cell Organelles Fluorescence. Taylor & Francis Group, 2003.

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Hirschberg, Joseph G., Rene Santus, Nuri Ozkutuk, and Elli Kohen. Atlas of Cell Organelles Fluorescence. Taylor & Francis Group, 2003.

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Book chapters on the topic "Cytofluorimetrie"

1

Dahlström, Annica, and Pär-Anders Larsson. "Cytofluorimetric Scanning: A Quantitative Method to Study Axonal Transport." In Quantitative Neuroanatomy in Transmitter Research, 317–29. London: Palgrave Macmillan UK, 1985. http://dx.doi.org/10.1007/978-1-349-08171-4_21.

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Dahlström, Annica, and Pär-Anders Larsson. "Cytofluorimetric Scanning: A Quantitative Method to Study Axonal Transport." In Quantitative Neuroanatomy in Transmitter Research, 317–29. Boston, MA: Springer US, 1985. http://dx.doi.org/10.1007/978-1-4613-2139-2_21.

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3

Larsson, P. A., S. Bööj, K. Lundmark, M. Goldstein, and A. Dahlström. "Cytofluorimetric Scanning Studies on Axonal Transport in Reserpinized Adrenergic Nerves." In Histochemistry and Cell Biology of Autonomic Neurons and Paraganglia, 282–87. Berlin, Heidelberg: Springer Berlin Heidelberg, 1987. http://dx.doi.org/10.1007/978-3-642-72749-8_50.

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Budikhina, Anna, and Boris Pinegin. "Evaluation of Bactericidal Activity of Human Biological Fluids by Flow Cytofluorimetry." In Advances in Experimental Medicine and Biology, 307–10. New York, NY: Springer New York, 2007. http://dx.doi.org/10.1007/978-0-387-72005-0_33.

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5

Vivar, Omar I., Joao G. Magalhaes, and Sebastian Amigorena. "Measurement of Export to the Cytosol in Dendritic Cells Using a Cytofluorimetry-Based Assay." In Methods in Molecular Biology, 183–88. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3606-9_13.

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"Cytometry, Cytofluorimetry, Microfluorimetry, Analytical Cytology." In Encyclopedia of Systems Biology, 516. New York, NY: Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4419-9863-7_100307.

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Palermo, Belinda, Mariangela Panetta, Giulia Campo, and Paola Nisticò. "A cytofluorimetric assay to evaluate T cell polyfunctionality." In Methods in Enzymology, 61–76. Elsevier, 2020. http://dx.doi.org/10.1016/bs.mie.2019.07.041.

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Sordo-Bahamonde, Christian, Seila Lorenzo-Herrero, Segundo González, and Alejandro López-Soto. "A cytofluorimetric assay to evaluate intracellular cytokine production by NK cells." In Methods in Enzymology, 343–55. Elsevier, 2020. http://dx.doi.org/10.1016/bs.mie.2019.05.049.

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MELINO, G., V. De LAURENZI, I. SAVINI, and P. GUERRIERI. "Cytofluorimetric Assessment of Cellular Peroxidative Activity Using 2′, 7′-Dichlorofluorescin Diacetate." In Protides of the Biological Fluids, 459–67. Elsevier, 1989. http://dx.doi.org/10.1016/b978-0-08-037378-2.50064-4.

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Conference papers on the topic "Cytofluorimetrie"

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Puxeddu, Ermanno, Daniela Fraboni, Floriana Bardaro, Francesco Buccisano, Gabriella Lucà, and Paola Rogliani. "Cytofluorimetric BAL cells characterization in IPF and in other ILD." In ERS International Congress 2016 abstracts. European Respiratory Society, 2016. http://dx.doi.org/10.1183/13993003.congress-2016.pa3895.

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Pidard, D., A. Fischer, C. Bouillot, F. Ledeist, and A. T. Nurden. "INHERITED DEFICIENCIESCAN AFFECT SEPARATELY THE PLATELET MEMBRANE GLYCOPROTEIN Ilb-IIIa COMPLEX AND THE LEUKOCYTE LFA-1, Mac-1 and pl50,95 COMPLEXES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643704.

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The human platelet membrane glycoprotein (GP) IIb-IIIa complex and a family of functional leukocyte cell membrane antigens, LFA-1 (L), Mac-1 (M) andpl50,95 (X), possess knownstructural analogies. Similaritiesinclude a heterodimeric structure with a high mol. wt. αsubunit (Mr∽ 145-180 kDa) , associated nonconvalently with a lower mol. wt.β-subunit (Mr ∽ 90-95 kDa),anda partial amino acid sequence hommology between GP lib and αL or CKM. Furthermore, GP lib, α L and αM were reported to be co-expressed in murine cells transfected with a 20 kilobase human DNA fragment.To address the question of a possible genomic linkage between these related glycoproteins of different cells, we have examined patients with either ‘LFA-1 immunodeficiency disease’ or with Glanzmann's thrombasthenia (GT). S.Bo. and P.Ce. are children affected by recurrent bacterial infections since birth. Cytofluorimetric analysis using monoclonal antibodies (MAbs) for the αL, αM,αX and β subunits demonstrated that the surface expression of LFA-1, Mac-1 and pl50,95 intheir leukocytes was ≤1% of the normal values. Platelets from both patients aggregated normally and readily bound the MAbs AP-2 (anti-GP IIb-IIIa) or Tab (anti-GP IIb). Crossed immunoelectrophoresis confirmed the presenceof GP IIb-IIIa complexes, whileSDS-polyacrylamide gel electrophoresis showed a normal migration of GP IIb and GP IIIa. Patients C. Bl. and M.Ca.are typical of the type I subgroupofGT. Their platelets are unable toaggregate and contain < 1% of the normal platelet content of GP liband GP Ilia.Cytofluoro-graphy showed a normal expression of LFA-1, Mac-1 and 150,95 on the surface of lymphocytes, polymorphonuclear cells and/or monocytes of both patients. Our data thus show that despite sequence homologies and a potential genomic linkage, the cellular expression of the platelet GP IIb-IIIa complex and of the LFA-1 related leukocyte antigens may be dissociated in genetic disorders where anabnormal glycoprotein distribution may be restricted to one type of cell.

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