Relevant bibliographies by topics / Cytofluorimetry

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers

Academic literature on the topic 'Cytofluorimetry'

Author: Grafiati
Published: 4 June 2021
Last updated: 14 March 2023

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Journal articles on the topic "Cytofluorimetry"

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Kit, O. I., N. K. Guskova, O. N. Selyutina, V. V. Dmitrieva, I. A. Novikova, I. S. Torpujyan, and V. R. Zakharchenko. "FEATURES OF A DIFFERENTIAL DIAGNOSIS OF PEDIATRIC ACUTE MONOCYTIC LEUKEMIA (AML-M5a) ON THE EXAMPLE OF A CLINICAL CASE." South Russian Journal of Cancer 1, no. 1 (March 7, 2020): 76–83. http://dx.doi.org/10.37748/2687-0533-2020-1-1-7.

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The aim of this work was to assess the significance of investigating clinical and laboratory parameters for diagnosing acute monocytic leukemia in children on the basis of a clinical case. The article demonstrates specific features of differentiating AML M5a from other forms of acute myeloid leukemia. According to the results of hematological, morphological and cytofluorimetric studies of blood and bone marrow samples, the diagnosis of acute myeloid leukemia was established. The morphological and phenotypic characteristics of blast cells hampered the diagnosis of an AML form. However, a comprehensive analysis of the expressed CD antigens allowed acute monocytic leukemia to be identified, which diagnosis was subsequently confirmed by a cytochemical study. Thus, the clinical diagnosis was established over a short period of time. This was of importance given the rising severity of the patient’s condition requiring immediate treatment, the initial hyperleukocytosis and the development of life-threatening complications associated with leukostasis in the lungs and the central nervous system. In the presented case, the clinical manifestations of the underlying disease and the results of flow cytofluorimetry were determining factors in initiating timely specific therapy.
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Mbida, A. D., B. Pozzetto, O. Sabido, Y. Akono, F. Grattard, M. Habib, and O. G. Gaudin. "Competition binding studies with biotinylated echovirus 11 in cytofluorimetry analysis." Journal of Virological Methods 35, no. 2 (November 1991): 169–76. http://dx.doi.org/10.1016/0166-0934(91)90132-j.

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Sabido, Odile, D. Mbida André, Bruno Pozetto, Odette Gaudin, and François Berthoux. "COMPETITION BINDING STUDIES WITH BIOTINYLATED ECHOVIRUS 11 IN CYTOFLUORIMETRY ANALYSIS." Biology of the Cell 73, no. 2-3 (January 1991): 47a. http://dx.doi.org/10.1016/0248-4900(91)90252-i.

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Jacob, Marie-Christine, Mireille Favre, and Jean-Claude Bensa. "EVALUATION OF CD4 CD45RA LYMPHOCYTES IN MALIGNANT LYMPHOMA BY CYTOFLUORIMETRY." Biology of the Cell 73, no. 2-3 (January 1991): 50a. http://dx.doi.org/10.1016/0248-4900(91)90263-m.

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Dimitropoulos, K., J. M. Rolland, and R. C. Nairn. "Flow cytofluorimetry of fluorescein fluorescence polarization to assay lymphocyte activation." Biochemical and Biophysical Research Communications 136, no. 3 (May 1986): 1021–29. http://dx.doi.org/10.1016/0006-291x(86)90435-3.

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Winstanley, F. P. "Detection of TNF alpha receptors on ovine leucocytes by flow cytofluorimetry." Research in Veterinary Science 52, no. 1 (January 1992): 129–31. http://dx.doi.org/10.1016/0034-5288(92)90074-c.

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Ambrosio, Maria Rosaria, Giusy Mosca, Teresa Migliaccio, Domenico Liguoro, Gisella Nele, Fabrizio Schonauer, Francesco D’Andrea, et al. "Glucose Enhances Pro-Tumorigenic Functions of Mammary Adipose-Derived Mesenchymal Stromal/Stem Cells on Breast Cancer Cell Lines." Cancers 14, no. 21 (November 3, 2022): 5421. http://dx.doi.org/10.3390/cancers14215421.

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Adiposity and diabetes affect breast cancer (BC) progression. We addressed whether glucose may affect the interaction between mammary adipose tissue-derived mesenchymal stromal/stem cells (MAT-MSCs) and BC cells. Two-dimensional co-cultures and spheroids were established in 25 mM or 5.5 mM glucose (High Glucose-HG or Low Glucose-LG) by using MAT-MSCs and MCF7 or MDA-MB231 BC cells. Gene expression was measured by qPCR, while protein levels were measured by cytofluorimetry and ELISA. CD44high/CD24low BC stem-like sub-population was quantified by cytofluorimetry. An in vivo zebrafish model was assessed by injecting spheroid-derived labeled cells. MAT-MSCs co-cultured with BC cells showed an inflammatory/senescent phenotype with increased abundance of IL-6, IL-8, VEGF and p16INK4a, accompanied by altered levels of CDKN2A and LMNB1. BC cells reduced multipotency and increased fibrotic features modulating OCT4, SOX2, NANOG, αSMA and FAP in MAT-MSCs. Of note, these co-culture-mediated changes in MAT-MSCs were partially reverted in LG. Only in HG, MAT-MSCs increased CD44high/CD24low MCF7 sub-population and promoted their ability to form mammospheres. Injection in zebrafish embryos of HG spheroid-derived MCF7 and MAT-MSCs was followed by a significant cellular migration and caudal dissemination. Thus, MAT-MSCs enhance the aggressiveness of BC cells in a HG environment.
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Mustafin, I. G., V. Kh Fazylov, and A. O. Baryshnikov. "Phenotype of peripheral blood lymphocytes in angina." Kazan medical journal 81, no. 2 (February 2, 2022): 103–7. http://dx.doi.org/10.17816/kazmj96271.

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The phenotype of peripheric blood lymphocytes is studied in 87 patients with angina by cytofluorimetry. It is shown that the course of the disease is accompanied by significant changes of the phenotype of lymphocytes: the increase of expression of activation markers, the increase of the content of CD16+cells. However, the changes of the phenotype of lymphocytes in groups of patients with lacunar and ulceromembranous anginas are ambiguous in the acute period of the disease as well as late in the observation.
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Dini, L., A. Lentini, G. D. Diez, M. Rocha, L. Falasca, L. Serafino, and F. Vidal-Vanaclocha. "Phagocytosis of apoptotic bodies by liver endothelial cells." Journal of Cell Science 108, no. 3 (March 1, 1995): 967–73. http://dx.doi.org/10.1242/jcs.108.3.967.

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Using electron microscopy and cytofluorimetry we studied the role of carbohydrate-specific recognition systems in the interaction of apoptotic bodies with normal and interleukin 1-activated sinusoidal endothelial cells. Microfluorimetric observation of liver tissue sections revealed octadecylrhodamine B-labelled apoptotic body binding to the sinusoidal wall of mouse liver, when they were injected intraportally. Plate-scanning cytofluorimetry demonstrated that about 20–25% of Acridine Orange-labelled apoptotic bodies could adhere specifically to cultured endothelial cells after 15 minutes of incubation. Adhesion increased to 30% when the cells were incubated for 60 minutes. Using a mixture of galactose/N-acetylglucosamine/mannose as competition solution apoptotic body adhesion was significantly reduced especially after longer times of incubation, when the percentage of inhibition reached 50%. Following 4 hours exposure of liver endothelial cells to 1 ng/ml human recombinant interleukin-1 beta adhesion markedly increased after 60 minutes of incubation, whereas the co-incubation of interleukin-1 beta with the inhibitors brings down the adhesion to basal values obtained in controls. Electron microscopic observation of the adhesion process showed that the number of endothelial cells binding apoptotic bodies gradually increased from low to high values with time. After 60 minutes of incubation, the majority of apoptotic bodies were seen inside phagosomes and only a few remained at the cell surface. Liver endothelial cells bound and endocytosed apoptotic bodies through carbohydrate-specific receptors. Moreover, this scavenger action was interleukin-1 enhanced, thus suggesting its possible activation during inflammatory and immune processes.
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Pistillo, M. P., P. L. Tazzari, O. Mazzoleni, A. Urlacher, M. Falco, M. Vitale, R. W. Karr, and G. B. Ferrara. "ANALYSIS OF HLA SPECIFICITY OF HUMAN MONOCLONAL ANTIBODIES BY CYTOFLUORIMETRY AND CELL ELISA." European Journal of Immunogenetics 18, no. 5-6 (October 1991): 345–53. http://dx.doi.org/10.1111/j.1744-313x.1991.tb00034.x.

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Dissertations / Theses on the topic "Cytofluorimetry"

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Montini, Marco. "Assaying the order of transcriptional events in cells through CRISPR/Cas9." Doctoral thesis, Università di Siena, 2019. http://hdl.handle.net/11365/1073088.

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Cells and organisms are complex systems whose life and development result from the action of entangled cellular pathways and machineries. Phenotypes are triggered by the order in which cellular events occur. Yet, while interrogating individual events is relatively straightforward, it is difficult to assess the specific order and relevance of events when several cooperate to induce different phenotypes. Recently, new techniques greatly improved our ability to obtain information from cells by enabling the longitudinal tracking of molecular events into genomic DNA. Yet, these tools are still unable to relay the order in which different events occur. During my PhD I set up a system to assess the order of two transcriptional events leading to a specific phenotype in mammalian cells. To this aim I am exploited the CRISPR/Cas9 system to trigger the recombination of an artificial DNA cassette in which barcode sequences are interspersed with two sets of guideRNA targets. The transcription of Cas9 and of two sgRNAs induces double-strand breaks and the recombination of the cassette, depending upon the order in which the sgRNAs have been transcribed. Sequencing of the recombined cassette thus allows to ascertain the sequence of recombinational events leading to the recombination (i.e., the order in which the sgRNAs were used to target the cassette), which in turn acts as a proxy of the order of the cellular events leading to the expression of the individual sgRNAs. I have obtained proof of principle of my assay. Having obtained a stable cell line carrying a single copy of the reporter cassette, I sequentially expressed in HEK293T cells the individual Cas9/sgRNA pairs, using different orders of expression. I then recovered the cells and amplified the reporter cassette: Sanger sequencing of the barcodes present in the recombined cassette confirmed that the system worked as intended. In order to simplify the system and make it more robust, I have redesigned the cassette by replacing the DNA barcodes with cassettes driving the expression of four fluorescent reporters. This allows me to visualize the recombination outcome by flow cytometry using live cells: only two fluorescent reporters are expressed in the cells in their initial status, and the sequential recombination of the artificial cassette will drive the expression of the other fluorescent proteins. Each combination of fluorescence reflects a well-defined order of transcriptional activation of the guideRNAs. Beyond making the procedure more straightforward, the use of fluorescent reporters and flow cytometry allow me to assess each cell individually. A defined order of activation for these factors could explain the differences observed in re-programmability among cells, and could be exploited to improve our reprogramming techniques.
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Sève, Annie-Pierre. "Lectines nucleaires." Orléans, 1987. http://www.theses.fr/1987ORLE2020.

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Bouchaour, Ibrahim. "Etude de variants androgénétiques de Nicotiana plumbaginifolia Viv. Obtenus à partir de plantes haploides : Caractérisations, culture de protoplastes et régénération." Dijon, 1992. http://www.theses.fr/1992DIJOPE03.

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Loureiro, Luís Carlos Sá. "Functional characterization of mitochondrial suspensions by cytofluorimetric techniques towards the screening of mitochondrial target drugs." Master's thesis, 2015. http://hdl.handle.net/1822/35712.

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Dissertação de mestrado em Bioquímica Aplicada
Besides its role in respiration, mitochondria control other key metabolic pathways, cell proliferation and regulated cell death, with a crucial role in the regulation of the intrinsic apoptotic pathway. Recent studies have focused on the screen for anti-cancer agents that induce apoptosis through permeabilization of the mitochondrial outer membrane, leading to the cytosolic release of pro-apoptotic proteins and to the activation of the caspase cascade and consequently to cell dismantling. In the present study, staining protocols were developed and optimized aiming at the structural and functional characterization of isolated mitochondrial populations from yeast cells and rat liver by flow cytometry. Mitotracker Green and Nonyl Acridine Orange were used to measure changes in mitochondrial mass, 3,3'- Dihexyloxacarbocyanine Iodide and Mitotracker Red CMXROS to monitor the electrical potential of the inner membrane (Δψm), Mitotracker Red CM-H2XROS and Dihydroethidium to evaluate the accumulation of ROS, and 2',7'-Bis-(2-Carboxyethyl)- 5-(and-6)-Carboxyfluorescein and Acetoxymethyl Ester (BCECF-AM) to measure the pH of the mitochondrial matrix. Subsequent flow cytometry studies were designed to evaluate the effect in mitochondria function of two well-known apoptosis inducers in both mammalian cell lines and yeast cells, namely bovine lactoferrin (bLf) and acetate, and to understand whether or not they act directly on mitochondria independently of upstream cellular pathways. The results showed that both bLf and acetate promoted changes in mitochondrial mass, matrix mitochondrial pH, Δψm and ROS levels of rat liver mitochondria. Moreover, the assessment of the activity of the complexes of electron transport chain (ETC) showed that bLf and acetate affect their activity and trigger mitochondrial swelling. These results are particularly interesting since the two compounds appear to induce in isolated mitochondria mainly the same alterations as those occurring in whole cells, and which have been proposed to be involved in the induction of cell death. Altogether, this study show that flow cytometry is a valuable tool to study key mitochondrial functional parameters and to screen for mitochondriatargeted drugs, including the anticancer agents, such as acetate and bLf.
Para além do seu papel na respiração celular, as mitocôndrias controlam outros aspetos chaves do metabolismo, bem como a proliferação celular e a morte celular regulada, tendo um papel crucial na regulação da via apoptótica intrínseca. Estudos recentes têm-se focado na procura de agentes anticancerígenos capazes de induzir apoptose mediada pela permeabilização da membrana mitocondrial externa, conduzindo à libertação de proteínas pró-apoptóticas e à ativação da cascata de caspases e consequente desmantelamento celular. No presente estudo, foram desenvolvidos e otimizados protocolos de marcação com vista à caracterização estrutural e funcional por citometria de fluxo de populações de mitocôndrias isoladas de células de levedura e de hepatócitos de rato. As sondas Mitotracker Green e laranja de nonil-acridina foram usadas para medir variações da massa mitocondrial, o iodeto de 3,3'-Dihexiloxacarbocianina e o Mitotracker Red CMXROS para medir o potencial eléctrico da membrana mitocondrial interna (Δψm), o Mitotracker Red CM-H2XROS e dihidroetídeo para avaliar a acumulação de ROS e o acetoximetil éster de (2',7'-Bis-(2- Carboxietil)-5-(and-6)-carboxifluoresceína e(BCECF-AM) para medir o pH da matriz mitocondrial. Os estudos subsequentes de citometria de fluxo foram desenhados para avaliar o efeito de diferentes drogas na função mitocondrial, em particular da lactoferrina bovina (bLf) e do acetato, dois reconhecidos indutores de apoptose, no sentido de se compreender se atuam diretamente na mitocôndria independentemente de outras vias celulares a montante. Os resultados mostraram que tanto a bLf como o acetato promovem alterações na massa mitocondrial, pH da matriz mitocondrial, Δψm e nos níveis de ROS em mitocôndrias isoladas de fígado de rato. Além disso, a avaliação da atividade dos complexos da cadeia de transportadora de eletrões (ETC) mostrou que a bLf e o acetato afetam a sua atividade e desencadeiam a tumefação mitocondrial. Estes resultados são particularmente interessantes uma vez que os dois compostos parecem induzir, em mitocôndrias isoladas as mesmas alterações que ocorrem em células inteiras, e que têm sido propostas como estando envolvidas na indução da morte celular. No seu conjunto, este estudo mostra que a citometria de fluxo é uma ferramenta valiosa para o estudo da funcionalidade mitocondrial e para o rastreio de drogas que têm como alvo a mitocôndria, incluindo agentes anticancerígenos, tais como a bLf e o acetato.
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Books on the topic "Cytofluorimetry"

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Elli, Kohen, ed. Atlas of cell organelles fluorescence. Boca Raton: CRC Press, 2004.

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Hirschberg, Joseph G., Rene Santus, Nuri Ozkutuk, and Elli Kohen. Atlas of Cell Organelles Fluorescence. Taylor & Francis Group, 2003.

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Hirschberg, Joseph G., Rene Santus, Nuri Ozkutuk, and Elli Kohen. Atlas of Cell Organelles Fluorescence. Taylor & Francis Group, 2003.

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Hirschberg, Joseph G., Rene Santus, Nuri Ozkutuk, and Elli Kohen. Atlas of Cell Organelles Fluorescence. Taylor & Francis Group, 2003.

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Hirschberg, Joseph G., Rene Santus, Nuri Ozkutuk, and Elli Kohen. Atlas of Cell Organelles Fluorescence. Taylor & Francis Group, 2003.

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Hirschberg, Joseph G., Elli Kohen, and Ren Santus. Atlas of Cell Organelles Fluorescence. Taylor & Francis Group, 2004.

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Hirschberg, Joseph G., Rene Santus, Nuri Ozkutuk, and Elli Kohen. Atlas of Cell Organelles Fluorescence. Taylor & Francis Group, 2003.

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Book chapters on the topic "Cytofluorimetry"

1

Budikhina, Anna, and Boris Pinegin. "Evaluation of Bactericidal Activity of Human Biological Fluids by Flow Cytofluorimetry." In Advances in Experimental Medicine and Biology, 307–10. New York, NY: Springer New York, 2007. http://dx.doi.org/10.1007/978-0-387-72005-0_33.

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Vivar, Omar I., Joao G. Magalhaes, and Sebastian Amigorena. "Measurement of Export to the Cytosol in Dendritic Cells Using a Cytofluorimetry-Based Assay." In Methods in Molecular Biology, 183–88. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3606-9_13.

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Balestrieri, E., D. Grimaldi, F. Lamonaca, and S. Rapuano. "Expanding the Metrological and Operating Characteristics of Cytofluorimeters." In Lecture Notes in Electrical Engineering, 210–39. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-05167-8_13.

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Dahlström, Annica, and Pär-Anders Larsson. "Cytofluorimetric Scanning: A Quantitative Method to Study Axonal Transport." In Quantitative Neuroanatomy in Transmitter Research, 317–29. London: Palgrave Macmillan UK, 1985. http://dx.doi.org/10.1007/978-1-349-08171-4_21.

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Dahlström, Annica, and Pär-Anders Larsson. "Cytofluorimetric Scanning: A Quantitative Method to Study Axonal Transport." In Quantitative Neuroanatomy in Transmitter Research, 317–29. Boston, MA: Springer US, 1985. http://dx.doi.org/10.1007/978-1-4613-2139-2_21.

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Larsson, P. A., S. Bööj, K. Lundmark, M. Goldstein, and A. Dahlström. "Cytofluorimetric Scanning Studies on Axonal Transport in Reserpinized Adrenergic Nerves." In Histochemistry and Cell Biology of Autonomic Neurons and Paraganglia, 282–87. Berlin, Heidelberg: Springer Berlin Heidelberg, 1987. http://dx.doi.org/10.1007/978-3-642-72749-8_50.

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"Cytometry, Cytofluorimetry, Microfluorimetry, Analytical Cytology." In Encyclopedia of Systems Biology, 516. New York, NY: Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4419-9863-7_100307.

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Palermo, Belinda, Mariangela Panetta, Giulia Campo, and Paola Nisticò. "A cytofluorimetric assay to evaluate T cell polyfunctionality." In Methods in Enzymology, 61–76. Elsevier, 2020. http://dx.doi.org/10.1016/bs.mie.2019.07.041.

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Sordo-Bahamonde, Christian, Seila Lorenzo-Herrero, Segundo González, and Alejandro López-Soto. "A cytofluorimetric assay to evaluate intracellular cytokine production by NK cells." In Methods in Enzymology, 343–55. Elsevier, 2020. http://dx.doi.org/10.1016/bs.mie.2019.05.049.

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MELINO, G., V. De LAURENZI, I. SAVINI, and P. GUERRIERI. "Cytofluorimetric Assessment of Cellular Peroxidative Activity Using 2′, 7′-Dichlorofluorescin Diacetate." In Protides of the Biological Fluids, 459–67. Elsevier, 1989. http://dx.doi.org/10.1016/b978-0-08-037378-2.50064-4.

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Conference papers on the topic "Cytofluorimetry"

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Puxeddu, Ermanno, Daniela Fraboni, Floriana Bardaro, Francesco Buccisano, Gabriella Lucà, and Paola Rogliani. "Cytofluorimetric BAL cells characterization in IPF and in other ILD." In ERS International Congress 2016 abstracts. European Respiratory Society, 2016. http://dx.doi.org/10.1183/13993003.congress-2016.pa3895.

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Ball, J., M. Greaves, C. Jackson, J. Peel, and F. E. Preston. "DN-9693: A PHOSPHODIESTERASE INHIBITOR WITH A PLATELET MEMBRANE EFFECT." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643583.

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We have examined the effect of DN-9693 (piperidinyl - imidazo - quinazoline: Daiichi Seiyaku, Japan) a water soluble phosphodiesterase inhibitor on platelet aggregation, secretion and thromboxane B2 (TXB2) production. In platelet rich plasma and at concentrations of 2uM and 5uM the drug significantly inhibited aggregation induced by adenosine diphosphate, collagen and sodium arachidonate. TXB2 production and release of adenosine tri-phosphate and 14C 5-hydroxytryptamine were also significantly inhibited by the drug. Cyclic adenosine mono-phosphate accumulation was enhanced. Inhibition of ristocetin induced platelet agglutination was an unexpected finding and further experiments were undertaken to explore this. These suggested no specific effect against a plasma factor (von Willebrand factor) and reduced expression of the platelet membrane glycoprotein Ib was implicated. To investigate this further we examined the effect of DN-9693 on the binding of a monoclonal antibody (McAb) to platelet membrane glycoprotein lb (AN51). This was assessed by a FACS IV flow cytofluorimeter utilising a goat anti-mouse fluorescein isothiocyanate (FITC) labelled secondary antibody. Similar experiments were also performed with McAbs to the membrane glycoprotein complex IIb/IIIa (M148) and also to glycoprotein IIIa (C17). In platelet rich plasma, at concentrations which have been shown to inhibit aggregation, DN-9693 significantly reduced the mean fluorescence intensity of the cells coated with McAb AN51 in a dose related manner. This strongly suggested a drug effect against the glycoprotein Ib receptor site. Also, the drug appeared to enhance the binding of McAb C17 to glycoprotein IIIa. This study indicates that in addition to potent phosphodiesterase inhibitor activity, DN-9693 causes a platelet surface membrane change which is associated with reduced expression of membrane glycoprotein Ib.
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Pidard, D., A. Fischer, C. Bouillot, F. Ledeist, and A. T. Nurden. "INHERITED DEFICIENCIESCAN AFFECT SEPARATELY THE PLATELET MEMBRANE GLYCOPROTEIN Ilb-IIIa COMPLEX AND THE LEUKOCYTE LFA-1, Mac-1 and pl50,95 COMPLEXES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643704.

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The human platelet membrane glycoprotein (GP) IIb-IIIa complex and a family of functional leukocyte cell membrane antigens, LFA-1 (L), Mac-1 (M) andpl50,95 (X), possess knownstructural analogies. Similaritiesinclude a heterodimeric structure with a high mol. wt. αsubunit (Mr∽ 145-180 kDa) , associated nonconvalently with a lower mol. wt.β-subunit (Mr ∽ 90-95 kDa),anda partial amino acid sequence hommology between GP lib and αL or CKM. Furthermore, GP lib, α L and αM were reported to be co-expressed in murine cells transfected with a 20 kilobase human DNA fragment.To address the question of a possible genomic linkage between these related glycoproteins of different cells, we have examined patients with either ‘LFA-1 immunodeficiency disease’ or with Glanzmann's thrombasthenia (GT). S.Bo. and P.Ce. are children affected by recurrent bacterial infections since birth. Cytofluorimetric analysis using monoclonal antibodies (MAbs) for the αL, αM,αX and β subunits demonstrated that the surface expression of LFA-1, Mac-1 and pl50,95 intheir leukocytes was ≤1% of the normal values. Platelets from both patients aggregated normally and readily bound the MAbs AP-2 (anti-GP IIb-IIIa) or Tab (anti-GP IIb). Crossed immunoelectrophoresis confirmed the presenceof GP IIb-IIIa complexes, whileSDS-polyacrylamide gel electrophoresis showed a normal migration of GP IIb and GP IIIa. Patients C. Bl. and M.Ca.are typical of the type I subgroupofGT. Their platelets are unable toaggregate and contain < 1% of the normal platelet content of GP liband GP Ilia.Cytofluoro-graphy showed a normal expression of LFA-1, Mac-1 and 150,95 on the surface of lymphocytes, polymorphonuclear cells and/or monocytes of both patients. Our data thus show that despite sequence homologies and a potential genomic linkage, the cellular expression of the platelet GP IIb-IIIa complex and of the LFA-1 related leukocyte antigens may be dissociated in genetic disorders where anabnormal glycoprotein distribution may be restricted to one type of cell.
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