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Journal articles on the topic 'Enterococcus faecalis ATCC 29212'

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Published: 4 June 2025
Last updated: 15 July 2025

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1

Diani, Prisinda, S. Setiawan Ame, Fatriadi Fajar, and Lestari Dinda. "Antibacterial Potential Test ofBasil Leaf (Ocimum Basilicum) Against Enterococcus Faecalis ATCC29212." International Journal Of Medical Science And Clinical Research Studies 01, no. 04 (2021): 65–70. https://doi.org/10.47191/ijmscrs/v1-i4-04.

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&nbsp; <strong>Introduction:</strong>&nbsp;Bacteria and their virulence factors are the main causes of apical periodontitis after endodontic treatment<em>.</em>&nbsp;The bacteria that is often found and considered as pathogens in root canals after endodontic treatment is&nbsp;<em>Enterococcus faecalis</em>. The main goal of endodontic treatment is the elimination of microorganisms and preventing re-infection after root canal treatment which can be achieved through mechanical-chemical debridement using an irrigation solution. Basil leaves (<em>Ocimum basilicum</em>) which is an herbal plant are thought to have antibacterial compounds that can inhibit&nbsp;<em>Enterococcus faecalis</em>. This research was conducted to determine the antibacterial potential of the n-hexane extract of basil leaves against&nbsp;<em>Enterococcus faecalis</em>&nbsp;ATCC 29212.&nbsp;<strong>Methods:&nbsp;</strong>This experimental laboratory study tested the effectiveness of n-hexane extract of basil leaves (<em>Ocimum basilicum</em>) against&nbsp;<em>Enterococcus faecalis&nbsp;</em>ATCC 29212 using&nbsp;<em>disk diffusion</em>&nbsp;method with three times of repetition.&nbsp;<strong>Results:&nbsp;</strong>The results of this study indicated that the n-hexane extract of basil leaves did not have an inhibition zone against&nbsp;<em>Enterococcus faecalis&nbsp;</em>ATCC 29212.&nbsp;<strong>Discussion:&nbsp;</strong>The concentration of n-hexane extract of basil leaves was not directly proportional to the diameter of the resulting inhibition zone. This is due to several factors that can affect the test results.&nbsp;<strong>Conclusion:&nbsp;</strong>The n-hexane extract of basil leaves does not have antibacterial potential effect against&nbsp;<em>Enterococcus faecalis&nbsp;</em>ATCC 29212.
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2

Fajar, Fatriadi, Rizali Dani, Prisinda Diani, Suciati Ame, and Ayudina Raliska. "Antibacterial Potential Test of Ethanol Extract of Basic Leaf (Ocimum Basilicum) against Enterococcus Faecalis ATCC 29212." International Journal Of Medical Science And Clinical Research Studies 03, no. 01 (2023): 71–79. https://doi.org/10.5281/zenodo.7564336.

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<strong>Introduction:</strong>&nbsp;<em>Enterococcus faecalis&nbsp;</em>is a bacteria found in failed root canal treatment cases.&nbsp;<em>Enterococcus faecalis&nbsp;</em>can tolerate significant environmental changes and invade the dentinal tubules, so under certain conditions, irrigants cannot altogether remove them in the chemomechanical process<em>.&nbsp;</em>Basil (&nbsp;<em>Ocimum basilicum&nbsp;</em>) is a natural ingredient with many benefits and is known to have high antibacterial activity against Gram-positive and Gram-negative bacteria. This study aimed to determine the antibacterial effect of ethanol extract from basil leaves (&nbsp;<em>Ocimum basilicum&nbsp;</em>) against&nbsp;<em>Enterococcus faecalis&nbsp;</em>ATCC 29212.&nbsp;<strong>Methods:&nbsp;</strong>This research was conducted by testing the bacteria&nbsp;<em>Enterococcus faecalis&nbsp;</em>ATCC 29212 with ethanol extract of basil (&nbsp;<em>Ocimum basilicum&nbsp;</em>) leaves made from 256,000 ppm &ndash; 1,000 ppm, using the&nbsp;<em>disc diffusion method</em>, carried out with three repetitions. Precise zone measurements were carried out using a caliper.<em>&nbsp;</em><strong>Results:&nbsp;</strong>There were no clear zones produced by the ethanol extract of basil leaves (&nbsp;<em>Ocimum basilicum&nbsp;</em>) against&nbsp;<em>Enterococcus faecalis&nbsp;</em>ATCC 29212 at various test concentrations.<em>&nbsp;</em><strong>Discussion:&nbsp;</strong>the test results in this study can be influenced by (i) the solubility factor of the extract, (ii) the active compounds contained in the plant, and (iii) the method used in this study.&nbsp;<strong>Conclusion:&nbsp;</strong>The ethanol extract of basil leaves (&nbsp;<em>Ocimum basilicum&nbsp;</em>) has no antibacterial potential against&nbsp;<em>Enterococcus faecalis&nbsp;</em>ATCC 29212. Further research can be carried out using the agar diffusion method or by conducting a minimum inhibitory concentration (MIC) test to confirm the antibacterial effect.
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3

Sovira, Gita Dwi Jiwanda, Mieke Hemiawati Satari, and Marry Siti Mariam. "Antimicrobial properties of various solvents combinations for phytochemical fraction derived from Uncaria gambier extract against Enterococcus faecalis ATCC 29212." Padjadjaran Journal of Dentistry 33, no. 1 (2021): 31. http://dx.doi.org/10.24198/pjd.vol33no1.25206.

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Introduction: Enterococcus faecalis is often found in root canal infections and can cause failure in root canal treatment. Uncaria gambier is an herbal medicine that is known to be rich in polyphenol compounds that have antibacterial properties. Because of the polyphenol content and antibacterial properties of Uncaria gambier, it can be an alternative as an antibacterial solution for root canal treatment. The purpose of this study is to analyze the antimicrobial of various solvents combinations for phytochemical fraction derived from Uncaria gambier extract against Enterococcus faecalis (ATCC 29212). Methods: This study is descriptive explorative research Uncaria gambier was extracted using a combination of three different fractions, ethyl acetate-water, hexane-water and hexane-ethyl acetate. We determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) against Enterococcus faecalis (ATCC 29212) using the serial microdilution method and by add test samples to the Mueller Hinton agar medium from each fraction Results: Combination ethyl acetate-water has MIC value at 0.195 mg/ml, combination hexane-water at 0.049 mg/ml and combination hexane-ethyl acetate have the MIC value at 1.563 mg/ml. The MBC value in combination ethyl acetate-water at 25 mg/ml, combination hexane-ethyl acetate and hexane-water MBC value at 50 mg/ml and 100 mg/ml. Based on optical density measurement using ELISA reader and bacterial growth on the media used, the fraction combinations increase the antibacterial effect of Uncaria gambier against E. faecalis ATCC 29212. Conclusion: The combination of various Uncaria gambier solvents has been shown to inhibit and kill E. faecalis (ATCC 29212). The combination of ethyl acetate-water fraction is the best combination to against E. faecalis (ATCC 29212). It means that the combination of various Uncaria gambier solvents can be developed as alternative root canal irrigation.
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4

Tkaczenko, Halina, Natalia Kurhaluk, Maryna Opryshko, et al. "ANTIBACTERIAL PROPERTIES OF COMMERCIAL PEPPERMINT ESSENTIAL OIL AGAINST SOME GRAM-POSITIVE AND GRAM-NEGATIVE BACTERIA." Аграрний вісник Причорномор'я, no. 109 (December 21, 2023): 27–36. http://dx.doi.org/10.37000/abbsl.2023.109.05.

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The authors of this article conducted research and studied the antibacterial properties of commercial peppermint essential oil (PEO) against several gram-positive and gram-negative bacteria provided by Polish manufacturers of essential oils (Naturalne Aromaty sp. z o.o., Klaj, Poland). Therefore, to conduct research with the aim of to study the antibacterial properties of commercial peppermint essential oil (PEO), an antimicrobial susceptibility test (Kirby-Bauer diffusion test) was used to measure diameters of bacterial growth inhibition zones). In the current study, Gram-negative strains such as Escherichia coli (Migula) Castellani and Chalmers (ATCC® 25922™ ), Escherichia coli (Migula) Castellani and Chalmers (ATCC® 35218™ ), Pseudomonas aeruginosa (Schroeter) Migula (ATCC® 27853™ ) and Gram-positive strains such as Staphylococcus aureus subsp. aureus Rosenbach (ATCC® 29213™ ), methicillin-resistant (MRSA), mecA positive Staphylococcus aureus (NCTC® 12493), Enterococcus faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz (ATCC® 51299™ ) (resistant to vancomycin; sensitive to teicoplanin) and Enterococcus faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz (ATCC® 29212™ ) were used. Results of the current study revealed that resistant to the PEO were Gram-negative bacterial strains, such as E. coli (Migula) Castellani and Chalmers (ATCC® 35218™ ) and P. aeruginosa (Schroeter) Migula (ATCC® 27853™ ) strains. The authors found that the diameters of the inhibition zones after application of PEO were similar to the control samples (96% ethanol). It was also found that after the application of REO, the increase in the diameters of the inhibition zones was 60.3% (p &lt; 0.05) for the Escherichia coli strain (Migula) Castellani and Chalmers (ATCC® 25922™ ) compared to control samples (96% ethanol ). Accordingly, Gram-positive strains such as S. aureus subsp. aureus Rosenbach (ATCC® 29213™ ) and methicillin-resistant S. aureus (NCTC® 12493) were equally resistant to PEO, similarly. On the other hand, Enterococcus faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz (ATCC® 29212™ ) and Enterococcus faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz (ATCC® 51299™ ) were sensitive to PEO. After the application of PEO, the largest diameters of inhibition zones were observed for the E. faecalis strain. The results suggest that commercial peppermint essential oil provided by Polish essential oil manufacturers (Naturalne Aromaty sp. z o.o., Kłaj, Poland) possesses some noteworthy antimicrobial properties. In vivo studies are necessary to calculate the effective dose of EOs and determine their possible side effects and toxicity.
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5

Wastri, Rahmi, Ame Suciati Setiawan, Dani Rizali Firman, Diani Prisinda, and Fajar Fatriadi. "Zona hambat ekstrak etil asetat daun kemangi (Ocimum basilicum) terhadap Enterococcus faecalis ATCC 29212Inhibition zone of ethyl acetate extract of basil leaves (Ocimum basilicum) against Enterococcus faecalis ATCC 29212." Jurnal Kedokteran Gigi Universitas Padjadjaran 33, no. 1 (2021): 8. http://dx.doi.org/10.24198/jkg.v33i1.28836.

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Pendahuluan: Enterococcus faecalis (E. faecalis) merupakan bakteri yang persisten pada saluran akar gigi, sehingga dapat menyebabkan infeksi sekunder pada saluran akar gigi yang telah diobturasi. Bahan irigasi dan medikamen harus diberikan pada saluran akar gigi untuk menunjang keberhasilan perawatannya. Daun kemangi (Ocimum basilicum/O. basilicum) merupakan tanaman obat tradisional yang diketahui memiliki potensi antibakteri dan berpeluang untuk dikembangkan sebagai bahan irigasi saluran akar gigi. Penelitian ini bertujuan untuk mengetahui zona hambat ekstrak etil asetat daun O. basilicum terhadap E. faecalis ATTC 29212. Metode:Jenis penelitian eksperimental laboratori. Pengujian zona hambat ekstrak etil asetat daun O. basilicum yang diperoleh dari proses maserasi dilakukan dengan metode difusi cakram dengan menggunakan klorheksidin 0,1% sebagai kontrol positif dan dilakukan tiga kali pengulangan terhadap E. faecalis ATCC 29212. Ekstrak pekat dilarutkan menggunakan DMSO 10% dan dilakukan pengenceran dua tingkat sehingga diperoleh konsentrasi ekstrak 512.000 – 1.000 ppm. Hasil: Ekstrak etil asetat daun O. basilicum pada konsentrasi uji 512.000 ppm telah memperlihatkan zona abu-abu seluas 1 mm di luar cakram uji, sementara konsentrasi uji lainnya tidak memperlihatkan adanya penghambatan. Beragam faktor dalam penelitian ini dapat mempengaruhi hasil pengujian, diantaranya adalah kelarutan ekstrak uji dalam pelarut yang digunakan, prosedur sebelum ekstraksi, dan keterbatasan metoda pengujian. Simpulan: Ekstrak etil asetat daun O. basilicum tidak memiliki zona hambat terhadap E. faecalis ATTC 29212.Kata kunci: Ocimum basilicum, Enterococcus faecalis ATCC 29212, zona hambat. ABSTRACTIntroduction: Enterococcus faecalis (E. faecalis) is a bacteria that persist in root canals, cause secondary infection in obturated root canals. Irrigation material and medicament must be administered to the root canal to support the treatment success. Basil leaves (Ocimum basilicum/O. basilicum) is a traditional medicinal plant known with antibacterial potential and has the opportunity to be developed as a root canal irrigation agent. This study was aimed to determine the inhibition zone of the ethyl acetate extract of O. basilicum leaves against E. faecalis ATTC 29212. Methods: This study was an experimental laboratory. The inhibition zone test of the ethyl acetate extract of O. basilicum leaves obtained from the maceration process was conducted with the disc diffusion method using 0.1% chlorhexidine as a positive control and three replications E. faecalis ATCC 29212. The concentrated extract was dissolved using 10% DMSO, and two dilution levels were carried out to obtain an extract concentration of 512,000 - 1,000 ppm. Results: The ethyl acetate extract of O. basilicum leaves at the test concentration of 512,000 ppm showed a grey zone of 1 mm outside the test disc, while other test concentrations did not show any inhibition. Various factors in this study can affect the test results, including the solubility of the test extract in the solvent used, the procedure before extraction, and the limitations of the test method. Conclusion: O. basilicum leaf ethyl acetate extract had no inhibition zone against E. faecalis ATTC 29212.Keywords: Ocimum basilicum, Enterococcus faecalis ATCC 29212, zone of inhibition.
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6

Ramírez-Condoy, Oliver, Lourdes Jerves-Andrade, Andrea Abril, and Marcela Redrován-Berrezueta. "Determinación de la actividad antibacteriana de extractos de plantas medicinales frente a Enterococcus faecalis." MASKANA 13, no. 2 (2022): 52–57. http://dx.doi.org/10.18537/mskn.13.01.07.

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Enterococcus faecalis es un microorganismo considerado como agente etiológico de diferentes procesos infecciosos, tales como: infecciones de la piel, osteomielitis, neumonía, infecciones del tracto urinario, endocarditis, entre otros, siendo los pacientes nosocomiales una población más vulnerable a esta bacteria. El objetivo del presente estudio fue evaluar la actividad antibacteriana in vitro de extractos metanólicos y clorofórmicos de las plantas Peperomia galioides, Otholobium mexicanum, Zingiber officinale y Rosmarinus officinalis frente a Enterococcus faecalis ATCC 29212, Enterococcus faecalis ATCC 51299 (resistente a la vancomicina) y una cepa clínica de Enterococcus faecalis. Para la evaluación de esta bioactividad se utilizó la metodología de microdilución y puntos de corte establecidos por la CLSI (2021) para Enterococcus faecalis y los resultados obtenidos se expresaron en IC50. El IC50 de mayor interés se obtuvo con el extracto clorofórmico de Otholobium mexicanum frente a la cepa clínica, siendo de 2.050 μg/mL, considerándose como muy activo. El IC50 más destacable de Peperomia galioides se obtuvo con el extracto clorofórmico frente a la cepa clínica con un valor de 8.248 μg/mL, considerándolo como ligeramente activo. Para Rosmarinus officinalis, solo el extracto metanólico mostró una ligera actividad antibacteriana con un IC50 de 20.234 μg/mL frente a Enterococcus faecalis ATCC 29212. Finalmente, todos los extractos de Zingiber officinale no mostraron actividad frente a las concentraciones probadas de las diferentes cepas de Enterococcus faecalis. Los resultados obtenidos son de gran relevancia ya que pueden ser aplicados para la producción de antimicrobianos a partir de fuentes naturales que podrían tener aplicación dentro de la industria farmacéutica, veterinaria o agrícola.
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7

Käbisch, Lisa, Anne-Kathrin Schink, Corinna Kehrenberg, and Stefan Schwarz. "Provisional Use of CLSI-Approved Quality Control Strains for Antimicrobial Susceptibility Testing of Mycoplasma (‘Mesomycoplasma’) hyorhinis." Microorganisms 9, no. 9 (2021): 1829. http://dx.doi.org/10.3390/microorganisms9091829.

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Antimicrobial susceptibility testing (AST) should be conducted in a standardized manner prior to the start of an antimicrobial treatment. For fastidious bacteria, such as porcine Mycoplasma (‘Mesomycoplasma’) spp., specifically M. hyorhinis, neither guidelines or standards for the performance of AST, nor quality control strains for the validation of AST results are approved by organizations like the Clinical and Laboratory Standards Institute (CLSI) or the European Committee on Antimicrobial Susceptibility Testing (EUCAST). The CLSI- and EUCAST-approved quality control strains Enterococcus faecalis ATCC 29212 and Staphylococcus aureus ATCC 29213 were chosen to validate AST by broth microdilution using modified Friis broth, developed as growth medium for porcine Mycoplasma (‘Mesomycoplasma’) spp. The antimicrobial agents doxycycline, enrofloxacin, erythromycin, florfenicol, gentamicin, marbofloxacin, tetracycline, tiamulin, tilmicosin, tulathromycin, and tylosin were examined using customized SensititreTM microtiter plates. Minimal inhibitory concentrations, determined after 24, 48, and 72 h, were mostly within the CLSI-approved quality control ranges for defined antimicrobial agents. We propose the use of the combination of E. faecalis ATCC 29212 and S. aureus ATCC 29213 as surrogate quality control strains for the validation of future AST results obtained for M. hyorhinis by broth microdilution using modified Friis broth.
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Prystowsky, Jason, Farida Siddiqui, John Chosay, et al. "Resistance to Linezolid: Characterization of Mutations in rRNA and Comparison of Their Occurrences in Vancomycin-Resistant Enterococci." Antimicrobial Agents and Chemotherapy 45, no. 7 (2001): 2154–56. http://dx.doi.org/10.1128/aac.45.7.2154-2156.2001.

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ABSTRACT To assess the potential for emergence of resistance during the use of linezolid, we tested 10 clinical isolates of vancomycin-resistant enterococci (VRE) (four Enterococcus faecalis, fiveEnterococcus faecium, and one Enterococcus gallinarum) as well as a vancomycin-susceptible control (ATCC 29212) strain of E. faecalis. The enterococci were exposed to doubling dilutions of linezolid for 12 passes. After the final passage, the linezolid plate growing VRE contained a higher drug concentration with E. faecalis than with E. faecium. DNA sequencing of the 23S rRNA genes revealed that linezolid resistance in three E. faecalis isolates was associated with a guanine to uracil transversion at bp 2576, while the one E. faecium isolate for which the MIC was 16 μg/ml contained a guanine to adenine transition at bp 2505.
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9

Yilmaz, Engin, and Alaaddin Cukurovali. "Synthesis, characterization, investigation of biological activity, docking studies, and spectroscopic properties of hydrazone compounds containing different substituents." Canadian Journal of Physics 97, no. 4 (2019): 408–16. http://dx.doi.org/10.1139/cjp-2018-0302.

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To assess electronic absorption spectra, molecular docking, and antibacterial activity, 12 new hydrazone compounds that have different substituents and in different positions were synthesized and characterized. HOMO–LUMO energies and theoretical electronic spectra of synthesized compounds were computed via Gaussian 09 software. Global reactivity descriptors were computed from HOMO–LUMO energies. In addition, 1JXA protein was used for molecular docking studies structure. Staphylococcus aureus ATCC 29213 and Enterococcus faecalis ATCC 29212 (as negative bacteria), Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853 (as gram positive) was selected. Compound 3 was observed to be the most effective against propagations of E. Coli. Similarly, compounds 7 and 9 were found to be the most effective against the reproduction of P. aeroginosa. The most powerful compound against the growth of E. faecalis was ascertained to be compound 7.
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10

Druzina, Anna A., Natalia E. Grammatikova, Olga B. Zhidkova, et al. "Synthesis and Antibacterial Activity Studies of the Conjugates of Curcumin with closo-Dodecaborate and Cobalt Bis(Dicarbollide) Boron Clusters." Molecules 27, no. 9 (2022): 2920. http://dx.doi.org/10.3390/molecules27092920.

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A series of novel conjugates of cobalt bis(dicarbollide) and closo-dodecaborate with curcumin were synthesized by copper(I)-catalyzed azide-alkyne cycloaddition. These conjugates were tested for antibacterial activity. It was shown that all derivatives are active when exposed to Bacillus cereus ATCC 10702 and are not active against Gram-negative microorganisms and Candida albicans at the maximum studied concentration of 1000 mg/L. The conjugate of alkynyl-curcumin with azide synthesized from the tetrahydropyran derivative of cobalt bis(dicarbollide) exhibited activity against Gram-positive microorganisms: Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212 and the clinical isolate MRSA 17, that surpassed curcumin by 2–4 times.
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Jarkhi, Alaa, Angeline Hui Cheng Lee, Zhenquan Sun, et al. "Antimicrobial Effects of L-Chg10-Teixobactin against Enterococcus faecalis In Vitro." Microorganisms 10, no. 6 (2022): 1099. http://dx.doi.org/10.3390/microorganisms10061099.

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Objective: Teixobactin and its analogues are a new class of antibiotics that have no detectable bacterial resistance. This study was designed to determine the antibacterial and antibiofilm activities of a novel teixobactin analogue, L-Chg10-teixobactin, against two strains of Enterococcus faecalis (E. faecalis). Materials and Methods: The efficacy of L-Chg10-teixobactin against two strains of E. faecalis (ATCC 29212 and 47077) was determined using Clinical and Laboratory Standards Institute methods. L-Chg10-teixobactin was prepared at a stock concentration of 1 mg/mL in 5% DMSO. The minimum inhibitory concentration (MIC) was calculated using a two-fold serial broth dilution method, utilizing a 96-well plate. The minimum bactericidal concentration (MBC) was determined by plating the bacteria onto agar to define the concentration that resulted in 99.9% of bacterial death. Ampicillin was used as the control. The effect of L-Chg10-teixobactin on the inhibition of ATCC 47077 strain biofilm formation was determined by measuring the minimum biofilm inhibitory concentration (MBIC) using the safranin assay, while the eradication of the preformed biofilm was determined by measuring the minimum biofilm eradication concentration (MBEC) using the XTT assay. For nonlinear data, the log dose–response curve was plotted to calculate the optimum concentration using Excel (version 16.51, Microsoft® excel. 2021, Microsoft Corporation, Reymond, WA, USA). The data are presented as mean ± standard deviation (SD). Results: The MIC and MBC values of L-Chg10-teixobactin against both strains of E. faecalis were 0.8 μg/mL. The MIC of ampicillin was 1.25 μg/mL for ATCC 29212 and ranged from 1.25 to 5 μg/mL for ATCC 47077. The MBC of ampicillin for ATCC 29212 and ATCC 47077 was 10 and 20 μg/mL, respectively. The MIC and MBC of ampicillin were much higher compared with those of L-Chg10-teixobactin. The MBEC80 of L-Chg10-teixobactin was 4.60 μg/mL for ATCC 47077, which was much lower than that of ampicillin (20 μg/mL). Conclusions:L-Chg10-teixobactin demonstrated potent antibacterial and antibiofilm effects against E. faecalis, suggesting its potential role an effective antibacterial and antibiofilm agent in endodontic treatment.
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Evranos-Aksöz, Begüm, Fatma Kaynak Onurdağ, and Selda Özgen Özgacar. "Antibacterial, antifungal and antimycobacterial activities of some pyrazoline, hydrazone and chalcone derivatives." Zeitschrift für Naturforschung C 70, no. 7-8 (2015): 183–89. http://dx.doi.org/10.1515/znc-2014-4195.

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Abstract Twenty-seven previously reported chalcones and their pyrazoline and hydrazone derivatives as well as two further chalcones have been screened for their antimicrobial, antifungal and antimycobacterial activities against standard microbial strains and drug resistant isolates. The minimum inhibitory concentration (MIC) value of each compound was determined by a two-fold serial microdilution technique. The compounds were found to possess a broad spectrum of antimicrobial activities with MIC values of 8–128 μg/mL. One compound [(E)-1-(4-hydroxyphenyl)-3-p-tolylprop-2-en-1-one] had equal activity with gentamycin (8 μg/mL) against Enterococcus faecalis. Chalcones were found to be more active than their hydrazone and 2-pyrazoline derivatives against Staphylococcus aureus ATCC 29213 and E. faecalis ATCC 29212.
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Kiymaci, ME, D. Simsek, KC Tok, D. Dirican, and M. Gumustas. "Probiotic potential and anti-quorum sensing activity of Enterococcus faecalis and Lactobacillus kunkeei isolates from Apis mellifera." Journal of the Hellenic Veterinary Medical Society 73, no. 4 (2023): 4717–28. http://dx.doi.org/10.12681/jhvms.26743.

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One health is an approach that gains importance in the protection of public health and advocates that the environment-animal-human health is linked. Microbial resistance occurring in any of these three factors circulates directly or indirectly between species and affects health. In this study, antimicrobial, anti-quorum sensing activity and potential probiotic properties of Lactobacillus acidophilus and Enterococcus faecalis 1 and 2 isolates from Apis mellifera gut were investigated. Results showed that isolates were found to be resistant to gentamicin, streptomycin, erythromycin, and clindamycin tested. It was determined that isolates were gamma haemolytic and resistant to acid and enzymatic environment conditions. Enterococcus faecalis isolates had antimicrobial activity on Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212 and Pseudomonas aeruginosa ATCC 27853 and anti-quorum sensing activity of the culture supernatants of all isolates was detected by a violacein pigment inhibition of Chromobacterium violaceum strain.
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Odland, Brant A., Meredith E. Erwin, and Ronald N. Jones. "Quality Control Guidelines for Disk Diffusion and Broth Microdilution Antimicrobial Susceptibility Tests with Seven Drugs for Veterinary Applications." Journal of Clinical Microbiology 38, no. 1 (2000): 453–55. http://dx.doi.org/10.1128/jcm.38.1.453-455.2000.

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ABSTRACT This multicenter study proposes antimicrobial susceptibility (MIC and disk diffusion methods) quality control (QC) parameters for seven compounds utilized in veterinary health. Alexomycin, apramycin, tiamulin, tilmicosin, and tylosin were tested by broth microdilution against various National Committee for Clinical Laboratory Standards (NCCLS)-recommended QC organisms ( Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Streptococcus pneumoniae ATCC 49619, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853). In addition, disk diffusion zone diameter QC limits were determined for apramycin, enrofloxacin, and premafloxacin by using E. coli ATCC 25922, P. aeruginosa ATCC 27853, and S. aureus ATCC 25923. The results from five or six participating laboratories produced ≥99.0% of MICs and ≥95.0% of the zone diameters within suggested guidelines. The NCCLS Subcommittee for Veterinary Antimicrobial Susceptibility Testing has recently approved these ranges for publication in the next M31 document.
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Salas, Fabiola, Judith Velasco, Janne Rojas, and Antonio Morales. "Antibacterial Activity of the Crude Extract and Constituents of Vismia Baccifera Var. Dealbata (Guttiferae) Collected in Venezuela." Natural Product Communications 2, no. 2 (2007): 1934578X0700200. http://dx.doi.org/10.1177/1934578x0700200216.

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The antibacterial activities of the crude extract of Vismia baccifera var. dealbata Triana &amp; Planch and of compounds isolated from it (sesamin, friedelin and vismiaquinone) were evaluated against Staphylococcus aureus (ATCC 25923), Enterococcus faecalis (ATCC 29212), Escherichia coli (ATCC 25992), Klebsiella pneumoniae (ATCC 23357), and Pseudomonas aeruginosa (ATCC 27853) using the disc diffusion agar method. The results obtained have revealed antibacterial activity against S. aureus, E. faecalis and P. aeruginosa, pathogen opportunists that become resistant to the antibiotics frequently used. According to the literature consulted, this is the first report of the antibacterial activity of the crude extract of V. baccifera and its constituents.
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Lukash, Oleksandr, Oleksandr Yakovenko, Halina Tkaczenko, and Natalia Kurhaluk. "IN VITRO ANTIBACTERIAL PROPERTIES OF NATURAL ACACIA HONEY AGAINST SOME GRAM-POSITIVE AND GRAM-NEGATIVE BACTERIAL STRAINS." Scientific and Technical Bulletin of the Institute of Animal Science NAAS of Ukraine, no. 131 (2024): 105–15. http://dx.doi.org/10.32900/2312-8402-2024-131-105-115.

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Acacia honey has a number of different medicinal properties. Its antiseptic properties are used to treat conjunctivitis and other eye conditions, as well as neurodermatitis and eczema. Used regularly, acacia honey helps to lower blood pressure. It has a mild sedative effect and is beneficial for mental disorders and nervous breakdowns. The aim of the present study was to evaluate the in vitro antimicrobial activity of natural acacia honey produced by a Polish manufacturer against Gram-positive strains such as Staphylococcus aureus subsp. aureus Rosenbach (ATCC® 25923™), Enterococcus faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz (ATCC® 51299™) (resistant to vancomycin; sensitive to teicoplanin) and Enterococcus faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz (ATCC® 29212™) and Gram-negative strains such as Pseudomonas aeruginosa (Schroeter) Migula (ATCC® 27853™), Escherichia coli (Migula) Castellani and Chalmers (ATCC® 25922™), Escherichia coli (Migula) Castellani and Chalmers (ATCC® 35218™). The antibacterial activity of natural acacia honey was tested in vitro using the Kirby-Bauer disc diffusion technique. The results of the current study showed that acacia honey samples produced by a Polish manufacturer exhibited strong antibacterial activity against Staphylococcus aureus subsp. aureus Rosenbach (ATCC® 35218™), E. faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz (ATCC® 51299™) and E. faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz (ATCC® 29212™) strains, with the mean zone of growth inhibition ranging from 20.1 to 35.45 mm. Pseudomonas aeruginosa (Schroeter) Migula (ATCC® 27853™), Escherichia coli (Migula) Castellani and Chalmers (ATCC® 25922™) and Staphylococcus aureus subsp. aureus Rosenbach (ATCC® 25923™) strains were resistant to acacia honey samples. The antibacterial properties of acacia honey need to be validated using model food systems. Further research could indicate whether honey has potential as a preservative in minimally processed foods.
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Saviano, Lorenzo, Antonietta Mancuso, Alice Cardito, et al. "Photocatalytic Degradation of Levofloxacin and Inactivation of Enterococci Levofloxacin-Resistant Bacteria Using Pure Rare-Earth Oxides." Separations 11, no. 9 (2024): 272. http://dx.doi.org/10.3390/separations11090272.

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In this study, La2O3 and CeO2 nanopowders were prepared using a simple and cost-effective precipitation method. Wide-angle X-ray diffraction (WAXD), UV-Visible reflectance diffuses (UV-Vis DRS), Raman spectroscopy, and specific surface area were used to characterize the photocatalysts, evidencing that the used preparation method was effective in the generation of crystalline CeO2 and La2O3. In particular, WAXD results showed that the average crystallite size of the achieved La2O3 and CeO2 samples were about 22 nm and 28 nm, respectively. The photocatalytic performances of the prepared catalysts were investigated in the degradation of levofloxacin (LEV) and the inactivation of a waterborne pathogen levofloxacin resistant (Enterococcus faecalis ATCC 29212) by using a photoreactor equipped with a solar simulator (SS). After 120 min, the CeO2 and La2O3 photocatalytic treatments allowed us to achieve between 75% and 83% of levofloxacin removal, respectively. A complete removal of 106 CFU/mL Enterococcus faecalis ATCC 29212 was achieved after 5 and 60 min of La2O3 and CeO2 photocatalytic processes, respectively.
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Dumitrascu, Florea, Ana-Maria Udrea, Mino R. Caira, et al. "In Silico and Experimental Investigation of the Biological Potential of Some Recently Developed Carprofen Derivatives." Molecules 27, no. 9 (2022): 2722. http://dx.doi.org/10.3390/molecules27092722.

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The efficient regioselective bromination and iodination of the nonsteroidal anti-inflammatory drug (NSAID) carprofen were achieved by using bromine and iodine monochloride in glacial acetic acid. The novel halogenated carprofen derivatives were functionalized at the carboxylic group by esterification. The regioselectivity of the halogenation reaction was evidenced by NMR spectroscopy and confirmed by X-ray analysis. The compounds were screened for their in vitro antibacterial activity against planktonic cells and also for their anti-biofilm effect, using Gram-positive bacteria (Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212) and Gram-negative bacteria (Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853). The cytotoxic activity of the novel compounds was tested against HeLa cells. The pharmacokinetic and pharmacodynamic profiles of carprofen derivatives, as well as their toxicity, were established by in silico analyses.
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Mora, Flor D., Yesenia L. Rojas, Viviana González, et al. "Chemical Composition and in vitro Antibacterial Activity of the Essential Oil of Verbesina negrensis from the Venezuelan Andes." Natural Product Communications 10, no. 7 (2015): 1934578X1501000. http://dx.doi.org/10.1177/1934578x1501000743.

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The composition and antimicrobial activity of the essential oil from Verbesina negrensis Steyerm. leaves is reported. Analysis was performed by GC/MS. Major constituents were α-pinene (43.1%), α-humulene (13.8%), δ-cadinene (8.1%), limonene (4.6%) and bicyclogermacrene (4.2%). The essential oil showed in vitro activity against the Gram-positive bacteria Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212 (MIC 500 and 350 μL/mL respectively).
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Silva, Jessica Stranburger da, Adriana Zilly, Rosane Meire Munhak da Silva, Carina Spoletto Librelotto, and Helder Ferreira. "Avaliação da atividade antibacteriana do óleo de girassol: Respaldo para a enfermagem." Research, Society and Development 10, no. 9 (2021): e8710917941. http://dx.doi.org/10.33448/rsd-v10i9.17941.

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Esta pesquisa objetivou avaliar a atividade antibacteriana do óleo de girassol. Trata-se de uma pesquisa experimental, desenvolvida em 2020. Para a avaliação da atividade antimicrobiana do óleo de girassol, foi utilizado o método disco de difusão em meio sólido e cepas bacterianas de Staphylococcus aureus, Enterococcus faecalis e klebsiela pneumoniae da American Type Culture Collection. O halo de inibição de crescimento bacteriano formado pelo óleo de girassol sobre a bactérias Staphylococcus aureus ATCC 25923 foi de 9 mm, para a Enterococcus faecalis ATCC 29212 foi de 11 mm e para a Klebsiela pneumoniae ATCC 13883 foi de 12 mm, conferindo atividade de inibição bacteriana. O estudo aponta que o óleo de girassol é uma matéria orgânica promissora, respaldando cientificamente seu uso pela equipe de enfermagem na assistência integral ao ser humano, como forma de prevenir infecções e recuperar a integridade cutânea.
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Prisinda, Diani, Ame Suciati Setiawan, and Fajar Fitriadi. "Antibacterial potential of Ocimum sanctum oils in relation to Enterococcus faecalis ATCC 29212." Dental Journal (Majalah Kedokteran Gigi) 51, no. 3 (2018): 104. http://dx.doi.org/10.20473/j.djmkg.v51.i3.p104-107.

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Background: Enterococcus faecalis is a Gram-positive cocci present in the root canal due to the failure of endodontic treatment and pulp tissue necrosis. The ideal root canal medicine offers biocompatible properties, ease of cleaning, absence of tooth staining and non-disruption of the root canal filling process. Basil (Ocimum sanctum) is one of the herbs widely used in salads which produces anti-bacterial, anti-fungal and anti-viral effects. The antibacterial effect of basil results from the eugenol which represents a main component demonstrating antibacterial properties. Basil essential oil has an antibacterial effect on both gram positive and gram-negative bacteria. Purpose: This study aimed to determine whether the essential oils contained in basil leaves offer any antibacterial potential with regard to the growth of Enterecoccus faecalis ATCC 29212. Methods: The research was experimental in nature incorporating a simple random sampling technique. In this study, groups of active substance compounds contained in basil leaves were extracted by distillation in order to obtain the essential oil. Preparation of the test solution involved essence of basil leaf oil at concentrations of 5,000 ppm, 10,000 ppm and 20,000 ppm in methanol solvent. A phytochemical test of basil was subsequently conducted in order to identify the content of the compound. The bacteria in this study was tested utilizing a disc diffusion method (Kirby and Bauer test) by measuring the diameter of the clear zone (clear zone) which is indicative of the bacterial growth inhibition response of antibacterial compounds in the extract. Results: The results of the research into the phytochemical test showed that basil contains phenolic flavonoids, triterpenoids, saponins, tannins which produce a negative result on steroids. The results of this study showed that the basil essential oil inhibition zone present in the Enterecoccus faecalis growth had a diameter of 11.70 mm at a concentration of 20,000 ppm. This concentration therefore proved most effective in relation to Enterococcus faecalis than other concentrations. Conclusion: It can be concluded that essential oils of basil leaves demonstrate anti-bacterial inhibitory properties with regard to Enterococcus faecalis.
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DeRyke, C. Andrew, Christina Sutherland, Bo Zhang, David P. Nicolau, and Joseph L. Kuti. "Serum Bactericidal Activities of High-Dose Daptomycin with and without Coadministration of Gentamicin against Isolates of Staphylococcus aureus and Enterococcus species." Antimicrobial Agents and Chemotherapy 50, no. 11 (2006): 3529–34. http://dx.doi.org/10.1128/aac.00290-06.

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ABSTRACT The purpose of this experiment was to evaluate the pharmacokinetics and serum bactericidal titers (SBTs) of daptomycin alone and in combination with gentamicin against strains of Staphylococcus aureus and enterococci to determine if there might be any benefit to the addition of the aminoglycoside. A multiple-dose, randomized crossover study was performed in 11 healthy volunteers to evaluate the steady-state pharmacokinetic profile of 6 mg/kg of body weight daptomycin once daily with or without 1 mg/kg gentamicin every 8 h. SBTs were determined against clinical isolates of nosocomial (MRSA 494) and community-acquired (CA-MRSA 44) methicillin-resistant S. aureus, vancomycin-susceptible Enterococcus faecalis (VSEF 49452), vancomycin-resistant Enterococcus faecium (VREF 80), and quality control strains of methicillin-susceptible S. aureus (ATCC 29213) and vancomycin-susceptible E. faecalis (ATCC 29212). Enhancement of bactericidal activity was evaluated by calculating and comparing the areas under the bactericidal curve (AUBC) for each dosing regimen against each isolate. The area under the concentration-time curve from 0 to 24 h and clearance for daptomycin alone were 645 ± 91 μg · h/ml and 9.47 ± 1.4 mg/h/kg, respectively, compared with 642 ± 69 μg · h/ml and 9.45 ± 1.0 mg/h/kg for daptomycin plus gentamicin. Daptomycin alone displayed sustained bactericidal activity against five of the six isolates over the entire 24-h dosing interval; bactericidal activity was maintained for 8 h against VREF 80. Mean AUBCs for daptomycin alone ranged from 935 to 1,263 and 36 to 238 against staphylococcal and enterococcal isolates, respectively, compared with 902 to 972 and 34 to 213 against staphylococci and enterococci when coadministered with gentamicin. The results of this study suggest that the addition of gentamicin does not alter the pharmacokinetic profile or enhance the bactericidal activity of daptomycin against staphylococcal or enterococcal isolates.
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Jerez Garzón, Belén Patricia, Nathaly Marcela Astudillo Arias, and Sandra Denisse Artega Sarmiento. "Efectividad de desinfectantes de uso hospitalario frente a Enterococcus faecalis." QhaliKay Revista de Ciencias de la Salud ISSN 2588-0608 7, no. 2 (2023): 134–41. http://dx.doi.org/10.33936/qkrcs.v7i2.6048.

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Los desinfectantes ejercen un rol importante al momento de mantener espacios libres de microorganismos que pueden resultar patógenos para la salud. Sin embargo, su efecto residual con el tiempo se ha visto reducido debido a diferentes causas inherentes a la bacteria o al compuesto químico como tal, lo cual puede provocar un aumento en la prevalencia de infecciones nosocomiales en los espacios hospitalarios. El objetivo es evaluar el efecto residual del etanol al 70º, glutaraldehído al 2%, clorhexidina 2% e hipoclorito de sodio 0,1% empleados como desinfectantes de uso hospitalario frente a Enterococcus faecalis, siendo este estudio de carácter descriptivo con enfoque cuantitativo y longitudinal. Se trabajó con Enterococcus faecalis ATCC-29212 mediante la técnica de Kirby Bauer en Agar Mueller Hinton. Se midieron los halos de inhibición obtenidos a los 20 minutos, una, tres, seis, 12 horas y 24 horas posteriores a la impregnación con el desinfectante para verificar su efecto residual. En los resultados se observó que Enterococcus faecalis ATCC 29212 no generó halos de inhibición frente a los dos primeros desinfectantes. Finalmente, el glutaraldehído al 2% y clorhexidina al 2% se mantuvieron con pequeños halos de inhibición durante los seis periodos, sin embargo, el efecto remanente no fue continuo, por ende, el efecto residual de estos biocidas resulta ineficaz en periodos prolongados de tiempo para limpieza y desinfección en ambientes hospitalarios.
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BRAQUEHAIS, I. D., F. R. VASCONCELOS, A. R. C. RIBEIRO, et al. "Estudo preliminar toxicológico, antibacteriano e fitoquímico do extrato etanólico das folhas de Jatropha mollissima (Pohl) Baill. (pinhão-bravo, Euphorbiaceae), coletada no Município de Tauá, Ceará, Nordeste Brasileiro." Revista Brasileira de Plantas Medicinais 18, no. 2 suppl 1 (2016): 582–87. http://dx.doi.org/10.1590/1983-084x/15_164.

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RESUMO A cada dia, cepas bacterianas estão tornando-se resistentes a diversos antibióticos, o que faz necessária a busca de novas substâncias eficazes para o tratamento de doenças. Desta forma, este trabalho reporta o estudo preliminar toxicológico, antibacteriano e fitoquímico do extrato etanólico das folhas de Jatropha mollissima (pinhão-bravo, Euphorbiaceae), coletada no Município de Tauá, Ceará, Nordeste Brasileiro. Inicialmente, realizou-se o teste de toxicidade do extrato contra Artemia salina. Na sequencia, foi realizado o ensaio antibacteriano contra quatro cepas bacterianas Gram-negativas (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Hafnia alvei ATCC 51873, Klebsiella pneumoniae ATCC 13883) e uma cepa Gram-positiva (Enterococcus faecalis ATCC 29212). Finalmente, fez-se a análise fitoquímica preliminar do extrato ativo para detecção das principais classes de metabólitos especiais. Como resultado, o extrato etanólico das folhas de J. mollissima se mostrou tóxico para Artemia salina, pois apresentou CL50 igual a 406,02 μg/mL. Quanto à ação antibacteriana, o extrato se mostrou ativo contra a bactéria Gram-positiva Enterococcus faecalis ATCC 29212, apresentando moderada atividade antibacteriana (halo de inibição igual a 7,03 mm). Evidenciou-se no extrato bioativo a presença de cumarinas, fenóis, taninos, flavonoides (flavonóis e flavanonas), alcaloides e esteroides, ambas as classes reportadas como antimicrobianos. Portanto, esse extrato tem potencial para ser usado na produção de fármacos contra infecções causadas por bactérias Gram-positivas. No entanto, as informações direcionam estudos futuros para o isolamento e identificação dos compostos bioativos, monitorados sob a ação antibacteriana mais expressiva.
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Souza, Rogério Emílio de, Eduardo Antônio de Souza, Manoel Damião Sousa-Neto, and Rosemeire Cristina Linhari Rodrigues Pietro. "In vitro evaluation of different chemical agents for the decontamination of gutta-percha cones." Pesquisa Odontológica Brasileira 17, no. 1 (2003): 75–78. http://dx.doi.org/10.1590/s1517-74912003000100014.

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This study evaluated the effectiveness of three disinfectants used in Dentistry for decontamination of gutta-percha cones. Sixty gutta-percha cones were contaminated with standardized pure cultures of five species of microorganisms (Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923, Candida albicans ATCC CBS-ICB/USP 562, Bacillus subtilis spores ATCC 6633 and Streptococcus mutans ATCC 25175). The cones were treated with 10% polyvinylpyrrolidone-iodine aqueous solution (PVP-I; Groups 1 and 2), 5.25% aqueous sodium hypochlorite (Groups 3 and 4) and paraformaldehyde tablets (Group 5). All chemical agents were efficient for the cold sterilization of gutta-percha cones in short time periods.
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26

Özbil, Ertuğrul. "In vitro Evaluation of Antibacterial Activity 2-benzoxazolinone and Its Combination Interaction with Ciprofloxacin." EMU Journal of Pharmaceutical Sciences 8, no. 1 (2025): 14–19. https://doi.org/10.54994/emujpharmsci.1687706.

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In the present study, it was aimed to demonstrate the antibacterial and synergistic activities of 2-benzoxazolinone, a promising allelochemical, against Klebsiella pneumoniae ATCC 700603, Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, and Staphylococcus aureus ATCC 25923 via broth microdilution method. 2-benzoxazolinone exhibited promising antibacterial activity against all tested bacteria with 4 mg/mL against E. coli and E. faecalis, and K. pneumoniae and S. aureus 2 mg/mL against. When used in combination with ciprofloxacin, no antagonistic activity was observed. However, the combination interaction revealed indifferent activity against all tested strains. Overall, the antibacterial data obtained from the study demonstrated that 2-benzoxazolinone can further be used as a potential antibacterial agent in the treatment of bacterial infections.
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27

Lilian Eiko MAEKAWA, Roberta LAMPING, Sidnei MARCACCI, Marcos Yasunori MAEKAWA, Maria Renata Giazzi NASSRI, and Cristiane Yumi KOGA-ITO. "Antimicrobial activity of chlorophyll-based solution on Candida albicans and Enterococcus faecalis." RSBO 4, no. 2 (2008): 36–40. http://dx.doi.org/10.21726/rsbo.v4i2.1294.

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Chlorophyll is a phytotherapic substance that presents curingproperties, however it is rarely used in Dentistry. Objective: Objective to analyzethe antimicrobial activity of a chlorophyll-based solution on isolatesof Candida albicans and Enterococcus faecalis by agar dilution method.Material and Methods: oral isolates and Material and Methods Candida albicans referencestrain (ATCC 18804) and Enterococcus faecalis (ATCC 29212) wereincluded in the study. Chlorophyll extract was diluted in Sabourauddextrose or brain heart infusion (BHI) agar, according to the testingmicroorganism and poured in Petri plates, obtaining the finalconcentrations of 50%, 25%, 12.5%, 6%, 3% and 1.5%. C. albicansand E. faecalis strains were plated with the aid of a Steers’ inoculator.Plates were incubated at 37°C for 48 h for C. albicans and at 5% CO2for E. faecalis. The experiments were performed in duplicate. Results: the results showed that the concentrations 50% and 25% chlorophyllextract were effective against C. albicans. E. faecalis grew in thepresence of all the concentrations. Conclusion: Conclusion: Conclusion: chlorophyll-basedsolution presents effective antimicrobial activity of C. albicans but didnot present any activity on E. faecalis.
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Seck, Insa, Samba F. Ndoye, Lalla A. Ba, et al. "Access to a Library of 1,3-disubstituted-1,2,3-triazenes and Evaluation of their Antimicrobial Properties." Current Topics in Medicinal Chemistry 20, no. 9 (2020): 713–19. http://dx.doi.org/10.2174/1568026620666200127143005.

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Background: Due to the rapid development of microbial resistance, finding new molecules became urgent to counteract this problem. Objective: The objective of this work is to access 1,2,3-triazene-1,3-disubstituted, a class of molecule with high therapeutic potential. Methods: Here we describe the access to 17 new triazene including six with an imidazole-1,2,3-triazene moiety and eleven with an alkyl-1,2,3-triazene moiety and their evaluation against five strains: two gram (-): Escherichia coli ATCC 25921 and Pseudomonas aeruginosa ATCC 27253; two gram (+) : Staphylococcus aureus ATCC 38213 and Enterococcus faecalis ATCC 29212; and one fungi: Candida albicans ATCC 24433. Results: All strains were sensitive and the best MIC, 0.28 µM, is observed for 4c against Escherichia coli ATCC 25921. Compound 9, 3-isopropynyltriazene, appears to be the most interesting since it is active on the five evaluated strains with satisfactory MIC 0.32 µM against Escherichia coli and Pseudomonas aeruginosa and 0.64 µM against Enterococcus faecalis and Pseudomonas aeruginosa. Conclusion: Comparing the structure activity relationship, electron withdrawing groups appear to increase antimicrobial activity.
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Moreno, José, Rosa Aparicio, Judith Velasco, Luis B. Rojas, Alfredo Usubillaga, and Marcó Lue-Merú. "Chemical Composition and Antibacterial Activity of the Essential Oil from Fruits of Bursera tomentosa." Natural Product Communications 5, no. 2 (2010): 1934578X1000500. http://dx.doi.org/10.1177/1934578x1000500229.

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The hydrodistilled oil from the fruits of Bursera tomentosa, obtained in 0.2% yield, was analyzed by GC-MS. Nine components were identified, which made up 99.3% of the oil. The most abundant constituents were cis-ocimene (47.6%), n-nonane (28.2%) and germacrene-D (11.1%). The oil showed antibacterial activity against Staphylococcus aureus (ATCC 25923), Enterococcus faecalis (ATCC 29212) and Salmonella typhi (CDC 57), with MIC values of 80 μg/mL, 120 μg/mL and 100 μg/mL, respectively.
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30

Carrillo-Tomalá, Celeste, Raúl Díaz-Torres, Katherine Guerra-Guamán, and Andrés Román-Salmerón. "Actividad antimicrobiana de extractos hidroalcohólicos de hojas de dos variedades de Mangifera indica L." CIENCIA UNEMI 13, no. 32 (2020): 69–77. http://dx.doi.org/10.29076/issn.2528-7737vol13iss32.2020pp69-77p.

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El objetivo de este trabajo fue evaluar la actividad antimicrobiana de extractos hidroalcohólicos (90% y 50%) de la variedad Tommy Atkins y extracto hidroalcohólico (50%) de la variedad Edward, seleccionados por su alto contenido de compuestos fenólicos, anteriormente publicado. Los extractos fueron obtenidos por maceración, digestión y ultrasonido. La actividad antimicrobiana se evalúo mediante los métodos Kirby Bauer y Kirby Bauer modificado; las cepas utilizadas fueron Pseudomona aeuroginosa (ATCC 27853), Staphylococcus aureus (ATCC 29213), Salmonella Typhimurium (ATCC 14028), Escherichia coli (ATCC 25922) y Enterococcus faecalis (ATCC 29212), ajustándose las suspensiones a turbidez 0.5 en escala McFarland. La mayor actividad antimicrobiana se evidencia frente a P. aeruginosa y S. aureus; los extractos de la variedad Tommy Atkins mostraron mayor actividad antimicrobiana, encontrándose halos de inhibición entre 10 y 15 mm según la bacteria. El método Kirby Bauer modificado mostró mayor efectividad. Se concluye que todas las cepas estudiadas presentaron sensibilidad frente a los extractos, siendo S. aureus y P. aeruginosa las más sensibles.
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Zeybek, E., A. Kart, and H. Yalcin. "Antimicrobial and antibiofilm forming activity of Origanum munzurense against some Gram-Positive bacteria and yeast." Journal of the Hellenic Veterinary Medical Society 73, no. 4 (2023): 4997–5004. http://dx.doi.org/10.12681/jhvms.28910.

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In vitro antimicrobial and antibiofilm forming activities of Origanum munzurense extract were investigated against some gram-positive bacteria and yeast strains. Liquid microdilution method and microplate method were used for antimicrobial and antibiofilm forming activities, respectively. Minimal inhibitory concentration values were found to be 2 mg/ml for Enterococcus faecalis ATCC 29212, 64 mg/ml for Listeria monocytogenes RSKK 02028, 2 mg/ml for L. monocytogenes RSKK 472, 2 mg/ml for Bacillus cereus NRRL 569 , 128 mg/ml for S. aureus ATCC 25923, 32 mg/ml for S. aureus (MRSA) ATCC 43300 strain, 64 mg/ml for Candida tropicalis ATCC 13803, 2 mg/ml for C. albicans ATCC 90028, 1 mg/ml for S. aureus FRI 918 and 1 mg/ml for Staphylococcus epidermidis ATCC 12228 strain. The extract prevented the biofilm formations of E. faecalis ATCC 29212, S. aureus FRI 918 and S.epidermidis ATCC 12228 at concentrations of 2 mg/ml, 500 µg/ml and 250 µg/ml, respectively. No biofilm formation was observed for the other bacteria tested.As a result, Origanum munzurense extract has antibacterial and antibiofilm activity on the selected pathogenic microorganisms tested. Due to these properties of O. munzurense, it could be considered as a plant-based antimicrobial agent to be used especially in the food industry.
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Iconaru, Simona-Liliana, Mikael Motelica-Heino, and Daniela Predoi. "Study on Europium-Doped Hydroxyapatite Nanoparticles by Fourier Transform Infrared Spectroscopy and Their Antimicrobial Properties." Journal of Spectroscopy 2013 (2013): 1–10. http://dx.doi.org/10.1155/2013/284285.

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Fourier transform infrared spectroscopy (FT-IR) analysis was conducted on europium-doped hydroxyapatite,Ca10-xEux(PO4)6(OH)2nanocrystalline powders (Eu:HAp) with0≤xEu≤0.2. Antimicrobial studies were also performed for the first time on Eu:HAp. The antimicrobial properties of Eu:HAp nanoparticles with0≤xEu≤0.2on Gram-negative (E. coli ATCC 25922,Pseudomonas aeruginosa 1397) and Gram-positive (Staphylococcus aureus 0364,Enterococcus faecalis ATCC 29212) bacteria systems and a species of fungus (Candida albicans ATCC 10231) were reported. Our study demonstrates that the antimicrobial activity of Eu:HAp nanoparticles is dependent on the europium concentration.
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Gallardo-Moreno, Amparo M., M. Luisa González-Martín, Ciro Pérez-Giraldo, José M. Bruque, and Antonio C. Gómez-García. "Serum as a Factor Influencing Adhesion of Enterococcus faecalis to Glass and Silicone." Applied and Environmental Microbiology 68, no. 11 (2002): 5784–87. http://dx.doi.org/10.1128/aem.68.11.5784-5787.2002.

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ABSTRACT The purpose of this work was to analyze the effect of serum on the physicochemical surface properties and adhesion to glass and silicone of Enterococcus faecalis ATCC 29212 at 37°C. As is presented using thermodynamics analysis, serum minimizes the interaction of cells with water, which correlates well with the increase in hydrophobicity and in bacterial adhesion to glass and silicone.
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34

Yılmaz, Fırat. "Assessment of Traditional and Commercial Rosehip Marmalade Samples: Physicochemical, Antioxidant, and Antibacterial Properties in Gümüşhane Province." Turkish Journal of Agriculture - Food Science and Technology 12, s2 (2024): 2317–27. https://doi.org/10.24925/turjaf.v12is2.2317-2327.7069.

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In this study, the specific physicochemical, antioxidant, and antibacterial properties of 20 different rosehip marmalade samples, produced using traditional and commercial methods in the Gümüşhane province and its districts, were comprehensively analyzed. To detect the chemical composition of the rosehip marmalade samples, analyses were conducted for total dry matter, pH, ash, titratable acidity (malic acid %), soluble solids, water activity, and color (L*, a*, b*). Additionally, alongside the physicochemical analyses, the contents of hydroxymethylfurfural (HMF) and sugars (fructose, glucose, sucrose, and total sugar) were also determined. To assess the antioxidant properties, analyses for total flavonoid content, total phenolic content, DPPH (% inhibition), and ABTS (% inhibition) were performed. Furthermore, the antibacterial activities of the rosehip marmalade samples against pathogenic bacterial strains such as Proteus vulgaris ATCC 29212, Enterococcus faecalis ATCC 29212, Klebsiella pneumoniae ATCC 13883, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Salmonella typhimurium ATCC 23566, and Escherichia coli O157:H7 35150 were investigated.
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Puca, Valentina, Tonino Traini, Simone Guarnieri, et al. "The Antibiofilm Effect of a Medical Device Containing TIAB on Microorganisms Associated with Surgical Site Infection." Molecules 24, no. 12 (2019): 2280. http://dx.doi.org/10.3390/molecules24122280.

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Surgical site infections (SSIs) represent the most common nosocomial infections, and surgical sutures are optimal surfaces for bacterial adhesion and biofilm formation. Staphylococcus spp., Enterococcus spp., and Escherichia coli are the most commonly isolated microorganisms. The aim of this research was to evaluate the antibiofilm activity of a medical device (MD) containing TIAB, which is a silver-nanotech patented product. The antibacterial effect was evaluated against Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, and E. coli ATCC 25922 by assessing the minimum inhibitory concentration (MIC) by the Alamar Blue® (AB) assay. The antibiofilm effect was determined by evaluation of the minimum biofilm inhibitory concentration (MBIC) and colony-forming unit (CFU) count. Subsequently, the MD was applied on sutures exposed to the bacterial species. The antimicrobial and antibiofilm effects were evaluated by the agar diffusion test method, confocal laser scanning microscopy (CLSM), and scanning electron microscopy (SEM). The MIC was determined for S. aureus and E. faecalis at 2 mg/mL, while the MBIC was 1.5 mg/mL for S. aureus and 1 mg/mL for E. faecalis. The formation of an inhibition zone around three different treated sutures confirmed the antimicrobial activity, while the SEM and CLSM analysis performed on the MD-treated sutures underlined the presence of a few adhesive cells, which were for the most part dead. The MD showed antimicrobial and antibiofilm activities versus S. aureus and E. faecalis, but a lower efficacy against E. coli. Surgical sutures coated with the MD have the potential to reduce SSIs as well as the risk of biofilm formation post-surgery.
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Kim, E. B., L. M. Kopit, L. J. Harris, and M. L. Marco. "Draft Genome Sequence of the Quality Control Strain Enterococcus faecalis ATCC 29212." Journal of Bacteriology 194, no. 21 (2012): 6006–7. http://dx.doi.org/10.1128/jb.01423-12.

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37

Manoil, Daniel, Ender Efe Cerit, Hong Fang, Stéphane Durual, Malin Brundin, and Georgios N. Belibasakis. "Profiling Antibiotic Susceptibility among Distinct Enterococcus faecalis Isolates from Dental Root Canals." Antibiotics 13, no. 1 (2023): 18. http://dx.doi.org/10.3390/antibiotics13010018.

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Enterococcus faecalis, a leading multi-resistant nosocomial pathogen, is also the most frequently retrieved species from persistently infected dental root canals, suggesting that the oral cavity is a possible reservoir for resistant strains. However, antimicrobial susceptibility testing (AST) for oral enterococci remains scarce. Here, we examined the AST profiles of 37 E. faecalis strains, including thirty-four endodontic isolates, two vanA-type vancomycin-resistant isolates, and the reference strain ATCC-29212. Using Etest gradient strips and established EUCAST standards, we determined minimum inhibitory concentrations (MICs) for amoxicillin, vancomycin, clindamycin, tigecycline, linezolid, and daptomycin. Results revealed that most endodontic isolates were susceptible to amoxicillin and vancomycin, with varying levels of intrinsic resistance to clindamycin. Isolates exceeding the clindamycin MIC of the ATCC-29212 strain were further tested against last-resort antibiotics, with 7/27 exhibiting MICs matching the susceptibility breakpoint for tigecycline, and 1/27 reaching that of linezolid. Both vanA isolates confirmed vancomycin resistance and demonstrated resistance to tigecycline. In conclusion, while most endodontic isolates remained susceptible to first-line antibiotics, several displayed marked intrinsic clindamycin resistance, and MICs matched tigecycline’s breakpoint. The discovery of tigecycline resistance in vanA isolates highlights the propensity of clinical clone clusters to acquire multidrug resistance. Our results emphasize the importance of implementing AST strategies in dental practices for continued resistance surveillance.
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Badica, Petre, Nicolae Dan Batalu, Mariana Carmen Chifiriuc, et al. "Sintered and 3D-Printed Bulks of MgB2-Based Materials with Antimicrobial Properties." Molecules 26, no. 19 (2021): 6045. http://dx.doi.org/10.3390/molecules26196045.

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Pristine high-density bulk disks of MgB2 with added hexagonal BN (10 wt.%) were prepared using spark plasma sintering. The BN-added samples are machinable by chipping them into desired geometries. Complex shapes of different sizes can also be obtained by the 3D printing of polylactic acid filaments embedded with MgB2 powder particles (10 wt.%). Our present work aims to assess antimicrobial activity quantified as viable cells (CFU/mL) vs. time of sintered and 3D-printed materials. In vitro antimicrobial tests were performed against the bacterial strains Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Enterococcus faecium DSM 13590, and Enterococcus faecalis ATCC 29212; and the yeast strain Candida parapsilosis ATCC 22019. The antimicrobial effects were found to depend on the tested samples and microbes, with E. faecium being the most resistant and E. coli the most susceptible.
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Aparicio, Rosa, María Cortez, Ysbelia Obregón, et al. "Tamizaje fitoquímico y actividad antibacteriana del extracto de las hojas de Espeletiopsis pannosa (Standl.) Cuatrec." Revista de la Facultad de Farmacia 66, no. 1 (2024): 32–37. https://doi.org/10.53766/refa/2024.66.01.04.

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Espeletiopsis pannosa (Standl.) Cuatrec., conocida como frailejón plateado, fue recolectada en los alrededores de la Laguna de Mucubaji, ubicada en el Parque Sierra Nevada del estado Mérida, Venezuela. Las hojas secas y molidas se extrajeron con hexano/éter (3:1) en Soxhlet a 50°C. Los metabolitos secundarios fueron determinados mediante pruebas químicas de coloración y/o precipitación. La actividad antibacteriana se evaluó a una concentración de 1000 ppm frente a Staphylococcus aureus (ATCC 25923), Enterococcus faecalis (ATTC 29212), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 23357) y Pseudomonas aeruginosa (ATCC 27853) por el método de difusión de agar con discos. Los resultados del tamizaje fitoquímico permitieron demostrar la presencia de diterpenos, triterpenos y esteroles. El extracto presentó actividad antibacteriana contra Staphylococcus aureus, Escherichia coli y Klebsiella pneumoniae con halos de inhibición de 7 mm para cada bacteria, mientras que Pseudomonas aeruginosa y Enterococcus faecalis fueron resistentes. Este es el primer estudio sobre el tamizaje fitoquímico y la actividad antibacteriana del extracto de las hojas de Espeletiopsis pannosa (Standl.) Cuatrec.
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40

Pratiwi, Ririn Dwi, Sri Kuswandari, and Rinaldi Budi Utomo. "Antibacterial effect of ethanol extract of moringa oleifera seeds against enterococcus faecalis atcc 29212." Odonto : Dental Journal 10, no. 2 (2023): 180. http://dx.doi.org/10.30659/odj.10.2.180-187.

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Background: One of the important stages in endodontic treatment is root canal irrigation. The most widely used irrigant is sodium hypochlorite (NaOCl), but it is toxic to periapical tissue. Irrigant solutions from natural ingredients that have antibacterial potential are needed, one of which is Moringa oleifera seeds. Bacteria that are often found persistent after root canal treatment are Enterococcus faecalis. The purpose of this study was to determine the antibacterial effect of the ethanol extract of M. oleifera seeds at concentrations of 37.5%, 50%, 67.5% and 75% against E. faecalis.Method: Experimental laboratory research with post-test only control group design was carried out with 4 times replication. Antibacterial activity was tested against E. faecalis ATCC 29212 using paper disc diffusion method on Mueller Hinton Agar (MHA) media. The diameter of the inhibition zone formed was measured. Data were analysed by one-way ANOVA followed by LSD.Result: The 2.5% NaOCl group produced the largest inhibition zone of 16.38 ± 0.95 mm, followed by the 75% extract group of 13.51 ± 0.49 mm, and the smallest was the 37.5% extract group of 4.42 ± 0.27 mm. The negative control (DMSO) did not produce an inhibition zone. The increase in the concentration of the ethanolic extract of M. oleifera seeds resulted in significantly better inhibiting the growth of E. faecalis (p&lt;0.05).Conclusion: Ethanol extract of M. oleifera seeds at 37.5%, 50%, 62.5% and 75% had antibacterial effects against E. faecalis, with the 75% had the strongest antibacterial effect compared to other extract concentrations.
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Torres Mantilla, José Diego. "Comparación del efecto antibacteriano de un extracto etanólico de propóleo a dos concentraciones y del paramonoclorofenol alcanforado frente a Enterococcus faecalis y Fusobacterium nucleatum." Revista Científica Odontológica 7, no. 1 (2019): 53–65. http://dx.doi.org/10.21142/2523-2754-0701-2019-53-65.

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Objetivo: Comparar el efecto antibacteriano in vitro de un extracto etanólico de propoleo a dos concentraciones frente a Enterococcus faecalis ATCC: 29212 y Fusobacterium nucleatum ATCC: 25586 con el paramonoclorofenol alcanforado (PMCFA). Materiales y método: Se incluyeron dos grupos de 15 placas Petri con cepas activadas de Enterococcus faecalis y Fusobacterium nucleatum. Se elaboró un extracto etanólico a partir de propoleo (EEP), proveniente de la provincia de Oxapampa (Perú), y se diluyo a concentraciones del 20% y el 30%. Se comparó su efecto antibacteriano frente al PMCFA, usando clorhexidina al 2% como control positivo y agua destilada como control negativo; mediante el método de Kirby-Bauer, en un periodo de 7 días para Fusobacterium nucleatum y 24 y 48 horas para Enterococcus faecalis. Se realizó el análisis estadístico mediante el programa SPSS versión 21. Resultados: Frente a Enterococcus faecalis se obtuvieron halos de 10,32 mm, 14,23 mm y 9,10 mm a las 24 horas y halos de 11 mm, 14,96 mm y 8,94 mm a las 48 horas, para las concentraciones de EEP al 20%, el 30% y el PMCFA, respectivamente. Por su parte, frente a Fusobacterium nucleatum, halos de 18,89 mm, 23,17 mm y 13,50 mm para las concentraciones al 20%, el 30% y el PMCFA, respectivamente. Conclusiones: El extracto etanólico elaborado a partir de propoleo de Oxapampa mostro efecto antibacteriano a una concentración del 20% y el 30%, que fue significativamente mayor al del PMCFA, frente a cepas activadas de Enterococcus faecalis y Fusobacterium nucleatum.
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42

Adu, Joseph Kwasi, Cedric Dzidzor Kodjo Amengor, Nurudeen Mohammed Ibrahim, et al. "Synthesis and In Vitro Antimicrobial and Anthelminthic Evaluation of Naphtholic and Phenolic Azo Dyes." Journal of Tropical Medicine 2020 (June 1, 2020): 1–8. http://dx.doi.org/10.1155/2020/4850492.

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The antimicrobial activity of 2-naphtholic and phenolic azo compounds was determined against seven microbial species, Staphylococcus aureus (ATCC 25923), Streptococcus pyrogenes (clinical), and Enterococcus faecalis (ATCC 29212), Salmonella typhi (clinical), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 251922), and Candida albicans (ATCC 10231), using the high-throughput spot culture growth inhibition assay (HT-SPOTi). The minimum inhibitory concentrations (MIC) were determined for the active azo dyes. All the azo compounds (A1–B4) were screened for anthelmintic activity against adult Ghanaian earthworms, Hyperiodrilus spp. As part of the systematic investigation for biological activity, all the azo compounds exhibited good antimicrobial activity against the seven human pathogenic microorganisms. All the compounds exhibited anthelminthic activity against adult Ghanaian earthworms, Hyperiodrilus spp.
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43

Villarreal, Silvana, María Villegas-Moreno, Luis Rojas, Yndra Cordero de Rojas, María Rodríguez-Arredondo, and David Castillo-Trujillo. "Composición química y evaluación de la actividad antibacteriana del aceite esencial de Tithonia diversifolia (Hemsl.) A. Gray (Asteraceae) recolectada en el estado Mérida – Venezuela." Acta Bioclínica 12, no. 23 (2022): 7–27. http://dx.doi.org/10.53766/acbio/2022.12.23.02.

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El aceite esencial de las hojas y flores frescas de Tithonia diversifolia (Hemsl.) A. Gray, obtenido por el método de Hidrodestilación utilizando la trampa de Clevenger. Se obtuvo 1,2 mL de aceite a partir de 1 kg de hojas y 0,6 mL de aceite en relación a 1,320 kg de flores, y se caracterizó por cromatografía de gases-espectrometría de masas (CG/EM), lográndose identificar como componentes mayoritarios: a-pineno (54,44 %), limoneno (19,26 %), trans-ß-ocimeno (10,90 %), a-farneseno (4,17 %) y el sabineno (4,03 %) para las hojas; y el a-pineno (53,55 %), limoneno (11,57 %), 2-4-hexadienal (8,99 %), trans-ß-ocimeno (4,42 %), 1,8-cineol (3,68 %), a-farneseno (3,17 %) y el terpineol (3,05 %) para las flores. La actividad antibacteriana se determinó por el método de difusión en agar con disco, frente a bacterias de referencia internacional Staphylococcus aureus (ATCC 27923), Enterococcus faecalis (ATCC 29212), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 23357), Pseudomona aeruginosa (ATCC 27853). El aceite esencial de las flores de T. diversifolia inhibió el crecimiento bacteriano de Escherichia coli con una CIM de 12,5 ppm y de Enterococcus faecalis con una CIM de 50 ppm.
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Villarreal, Silvana, Luis Beltrán, Yndra Cordero de Rojas, María Rodríguez-Arredondo, David Castillo-Trujillo, and María Villegas-Moreno. "Composición química y evaluación de la actividad antibacteriana del aceite esencial de Tithonia Diversifolia (HEMSL.) A. Gray (Asteraceae) recolectada en el estado Mérida - Venezuela." Acta Bioclínica 12, no. 24 (2022): 48–69. http://dx.doi.org/10.53766/acbio/2022.12.24.04.

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El aceite esencial de las hojas y flores frescas de Tithonia diversifolia (Hemsl.) A. Gray, obtenido por el método de Hidrodestilación utilizando la trampa de Clevenger. Se obtuvo 1,2 mL de aceite a partir de 1 kg de hojas y 0,6 mL de aceite en relación a 1,320 kg de flores, y se caracterizó por cromatografía de gases-espectrometría de masas (CG/EM), lográndose identificar como componentes mayoritarios: a-pineno (54,44 %), limoneno (19,26 %), trans-ß-ocimeno (10,90 %), a-farneseno (4,17 %) y el sabineno (4,03 %) para las hojas; y el a-pineno (53,55 %), limoneno (11,57 %), 2-4-hexadienal (8,99 %), trans-ß-ocimeno (4,42 %), 1,8-cineol (3,68 %), a-farneseno (3,17 %) y el terpineol (3,05 %) para las flores. La actividad antibacteriana se determinó por el método de difusión en agar con disco, frente a bacterias de referencia internacional Staphylococcus aureus (ATCC 27923), Enterococcus faecalis (ATCC 29212), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 23357), Pseudomona aeruginosa (ATCC 27853). El aceite esencial de las flores de T. diversifolia inhibió el crecimiento bacteriano de Escherichia coli con una CIM de 12,5 ppm y de Enterococcus faecalis con una CIM de 50 ppm.
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45

Ferreira, Peracio Rafael Bueno, Cíntia Sorandra Oliveira Mendes, Cínthia Gracielly Rodrigues, et al. "Antibacterial activity tannin-rich fraction from leaves of Anacardium humile." Ciência Rural 42, no. 10 (2012): 1861–64. http://dx.doi.org/10.1590/s0103-84782012005000080.

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In vitro tests conducted with extracts rich in tannins have identified several biological activities of this class of substance. Thus, this paper intends to evaluate the antibacterial activity of tannin-rich fraction obtained from leaf extracts of Anacardium humile A.St.-Hil. Extracts of A. humile leaves in 70% acetone were semi-purified with ethyl acetate and butanol. We quantified the total tannins of the semi-purified fractions, of the crude extract and of aqueous residues and then performed tests of the antibacterial activity of the tannins against Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 29212. All positive extracts underwent tannin isolation using a Sephadex LH-20 column. The tannins isolated from the samples were quantified and tested for the minimal inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC). The tannins from crude extracts, semi-purifications and residues showed inhibition of S. aureus growth with MIC=500 g mL-1. All tannin fractions showed MIC against all strains and MBC, except against E. faecalis. The tannin fractions from Anacardium humile have antibacterial activity against Staphylococcus aureus, Pseudomononas aeruginosa and Enterococcus faecalis and, therefore, may be promising for future synthesis of new antibacterial agents.
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46

Astuti, Ranggi Hardian Nugro, Karlina Samadi, and Eric Priyo Prasetyo. "Daya Antibakteri Ekstrak Daun Belimbing Wuluh (Averrhoa bilimbi linn) terhadap Bakteri Enterococcus faecalis (Antibacterial Activity of Averrhoa bilimbi linn Leaf Extract against Enterococcus Faecalis)." Conservative Dentistry Journal 6, no. 2 (2016): 93. http://dx.doi.org/10.20473/cdj.v6i2.2016.93-98.

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Background. The prevalence of Enterococcus faecalis bacterial infection caused the failure of root canal treatment between 24%-77%. This is due to various factors resistance and virulence of Enterococcus faecalis. This research to find alternative materials that have antibacterial properties and by utilizing natural ingredients that can later be used as a root canal irrigation. Antibacterial activity of the Averrhoa bilimbi linn leaf extract against Enterococcus faecalis bacteria can be determined by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Purpose. The aim of this study was to determine the concentration of Averrhoa bilimbi linn leaf extract that has antibacterial activity against bacteria Enterococcus faecalis. Method. This research is a labolatory experimental with post test only control group design which use diluted Enterococcus faecalis ATCC 29212 according Mc. Farland standard 1,5 x 108 CFU/ml. With treatment Averrhoa bilimbi linn leaf extract on concentration 50%, 45%, 40%, 35%, 30%, and 25% given to each of 0,05 ml Enterococcus faecalis and using Brain Heart Infusion Broth (BHIB) as planting media. Result. At the concentration 30% of Averrhoa bilimbi linn leaf extract, showed that colony’s growth less than 10%. At the concentration 35% was not revealed any bacterial growth. Conclusion. The Averrhoa bilimbi linn leaf extract has antibacterial effect on bacteria Enterococcus faecalis. The MIC was at 30% and MBC was at 35%.
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de Paula Nogueira Cruz, Felipe, Ailton Ferreira de Paula, Camila Tita Nogueira, et al. "Discovery of a Novel Lineage Burkholderia cepacia ST 1870 Endophytically Isolated from Medicinal Polygala paniculata Which Shows Potent In Vitro Antileishmanial and Antimicrobial Effects." International Journal of Microbiology 2021 (February 17, 2021): 1–17. http://dx.doi.org/10.1155/2021/6618559.

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In this study, we report the isolation and identification of an endophytic strain of Burkholderia cepacia (COPS strain) associated with Polygala paniculata roots. Polygala plants are rich sources of promising microbiomes, of which the literature reports several pharmacological effects, such as trypanocidal, antinociceptive, anesthetic, anxiolytics, and anticonvulsant activities. B. cepacia COPS belongs to a new sequence type (ST 1870) and harbors a genome estimated in 8.3 Mbp which exhibits the aminoglycosides and beta-lactams resistance genes aph(3′)-IIa and blaTEM-116, respectively. Analysis performed using MLST, average nucleotide identity, and digital DNA-DNA hybridization support its species-level identification and reveals its novel housekeeping genes alleles gyrB, lepA, and phaC. The root endophyte B. cepacia COPS drew our attention from a group of 14 bacterial isolates during the primary screening for being potentially active against Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Micrococcus luteus ATCC 9341, Escherichia coli ATCC 25922, and Candida albicans ATCC 10231 and exhibited the broad-spectrum activity against phytopathogenic fungi. In addition, COPS strain showed production of protease, lipase, and esterase in solid media, and its natural product extract showed potent inhibition against fungal plant pathogens, such as Moniliophthora perniciosa, whose antagonism index (89.32%) exceeded the positive control (74.17%), whereas Sclerotinia sclerotiorum and Ceratocystis paradoxa showed high percentages of inhibition (85.53% and 82.69%, respectively). COPS crude extract also significantly inhibited S. epidermidis ATCC 35984, E. faecium ATCC 700221 (MIC values of 32 μg/mL for both), E. faecalis ATCC 29212 (64 μg/mL), and S. aureus ATCC 25923 (128 μg/mL). We observed moderate antagonistic activity against A. baumannii ATCC 19606 and E. coli ATCC 25922 (both at 512 μg/mL), as well as potent cytotoxic effects on Leishmania infantum and Leishmania major promastigote forms with 78.25% and 57.30% inhibition. In conclusion, this study presents for the first time the isolation of an endophytic B. cepacia strain associated with P. paniculata and enough evidence that these plants may be considered a rich source of microbes for the fight against neglected diseases.
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Morais, Alcina M. M. B., Decha Kumla, Valter F. R. Martins, et al. "Monoterpene Hydroxy Lactones Isolated from Thalassiosira sp. Microalga and Their Antibacterial and Antioxidant Activities." Molecules 29, no. 21 (2024): 5175. http://dx.doi.org/10.3390/molecules29215175.

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Two monoterpenoid lactones, loliolide (1) and epi-loliolide (2), were isolated from the crude dichloromethane extract of a microalga, Thalassiosira sp.). The structures of loliolide (1) and epi-loliolide (2) were elucidated by 1D and 2D NMR analysis, as well as a comparison of their 1H or/and 13C NMR data with those reported in the literature. In the case of loliolide (1), the absolute configurations of its stereogenic carbons were confirmed by X-ray analysis, whereas those of epi-loliolide (2) were determined by NOESY correlations. Loliolide (1) and epi-loliolide (2) were tested for their growth inhibitory activity against two Gram-positive (Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212) and two Gram-negative (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853) bacteria, as well as one clinical isolate (E. coli SA/2, an extended-spectrum β-lactamase producer-ESBL) and two environmental isolates, S. aureus 74/24, a methicillin-resistant (MRSA), and E. faecalis B3/101, a vancomycin-resistant (VRE) isolates. The results showed that none of the tested compounds exhibited antibacterial activity at the highest concentrations tested (325 μM), and both revealed low antioxidant activity, with ORAC values of 2.786 ± 0.070 and 2.520 ± 0.319 µmol TE/100 mg for loliolide (1) and epi-loliolide (2), respectively.
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Piasecki, Bartłomiej, Anna Biernasiuk, and Agnieszka Ludwiczuk. "Anti-Coccal Activity and Composition of the Essential Oils and Methanolic Extracts Obtained from Brewing Quality Humulus lupulus L. Hop Pellets." Pharmaceuticals 16, no. 8 (2023): 1098. http://dx.doi.org/10.3390/ph16081098.

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This study examined the chemical composition and anti-coccal properties of essential oils and methanolic extracts of six different Humulus lupulus L. varieties from Poland: Iunga, Marynka, Sybilla, Magnum, Tradition and Chinook. The activity of an α-acid-enriched fraction of methanolic extracts was also studied. The chemical composition of essential oils and extracts was determined by gas chromatography–mass spectrometry (GC/MS) and liquid chromatography–mass spectrometry (LC/MS) techniques. The compounds characteristic to H. lupulus extracts include xanthohumol, α-acids, β-acids, and prenylated flavonoids. Essential oil compositions showed a high prevalence of monoterpene hydrocarbon, myrcene and sesquiterpene hydrocarbons, α-humulene and β-caryophyllene. The antimicrobial activity was investigated against eight human cocci pathogenic strains: Staphylococcus aureus MRSA (ATCC 43300), S. aureus MRSA (29213), S. aureus MSSA (ATCC 29213), S. epidermidis (ATCC 12228), Enterococcus faecalis (ATCC 29212), E. faecalis VRE (ATCC 51299), E. faecium (ATCC 19434) and Micrococcus luteus (ATCC 10240). The lowest minimum inhibitory concentrations (MIC) were obtained for extracts and essential oils from Iunga hop samples. Extracts were significantly more active than essential oils. The most susceptible strain to both essential oils and extracts was M. luteus, whilst the least susceptible was E. faecium. The antimicrobial activity correlated with a high concentration of xanthohumol of active extracts rather than with the content of α-acids. Xanthohumol showed considerable activity against MRSA with an MIC value of 3.9 µg/mL. The activity of the α-acid-enriched fraction was mediocre compared to the results of all extracts.
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Nasution, Minasari, Ameta Primasari, Ika Astrina, and Arfi Luthfiyah Siregar. "Potential Antibacterial of Binahong Leaf Extract Against Bacteria Enterococcus faecalis ATCC® 29212™ (in vitro)." Dentika: Dental Journal 25, no. 1 (2022): 35–41. http://dx.doi.org/10.32734/dentika.v25i1.7800.

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Binahong is a plant widely found in Indonesia and has been used for generations, but only by inheritance without much optimal research. Enterococcus faecalis is bacteria that cause many problems in the oral cavity such as periradicular lesion. The purpose of this study was to determine the zone of inhibition, Minimum Bactericidal Concentration (MBC), and Minimum Inhibitory Concentration (MIC) of Binahong leaf extract against bacteria Enterococcus faecalis ATCC® 29212TM concentrations of 100%, 90%, 80%, 70%, and 60%, chlorhexidine 0,2% as positive control and DMSO as a negative control. The experimental method used for the research with a post-test only control group design, pure bacteria prepared in the microbiology laboratory, and Binahong leaf extract used in the chemical laboratory of the Faculty of Mathematics and Natural Sciences (FMIPA) at the University of North Sumatera. Five treatments of Binahong leaf extract concentration were tested as samples four times. MBC and MIC measurements of Binahong leaf extract against Enterococcus faecalis ATCC® 29212TM were exchanged for each concentration. Mueller Hinton Agar (MHA) and Nutrient Broth incubated for 24 hours at 37°C. The ANOVA test data is analysed, followed by the Post Hoc test. The average inhibition zone of 100% concentration was 14.19 mm, 90% was 12.25 mm, 80% was (10.19 mm), 70% was 8.81 mm, 60% was 0.00 mm, chlorhexidine 0,2% was 20.88 mm, and DMSO was 0.00 m). The conclusion is that the best inhibition zone has a concentration of 100%, MBC was at 100%, and MIC was at 60%.
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