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1

Rasyiid, Mustafid, Rendi Mahadi, Krisnanda Surya Dharma, Lindia Anggraini, Rahma Nurdiyanti, and Tri Rini Nuringtyas. "Immunomodulatory and Antioxidant Activity of Green Grass Jelly Leaf Extract (Cyclea barbata Miers.) In Vitro." Advances in Tropical Biodiversity and Environmental Sciences 2, no. 1 (2018): 10. http://dx.doi.org/10.24843/atbes.2018.v02.i01.p03.

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Green grass jelly (Cyclea barbata Miers.) is known for its benefit to human health especially in supporting body’s immune system and wellness. This research aimed to determine immunomodulatory and antioxidant activity of green grass jelly leaf extracts in vitro. Old leaves were collected as sample then dried and ground to powder. The extraction was done with sohxletation using three different solvents, chloroform, ethyl acetate, and ethanol. The immunomodulatory activity was evaluated by treating the crude extracts at concentrations of 50, 100, and 500 mg/mL on macrophages of rat in vitro. The
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2

Pratiwi, Liza, Achmad Fudholi, Ronny Martien, and Suwidjiyo Pramono. "Ethanol Extract, Ethyl Acetate Extract, Ethyl Acetate Fraction, and n-Heksan Fraction Mangosteen Peels (Garcinia mangostana L.) As Source of Bioactive Substance Free-Radical Scavengers." JPSCR : Journal of Pharmaceutical Science and Clinical Research 1, no. 2 (2016): 71. http://dx.doi.org/10.20961/jpscr.v1i2.1936.

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<span>Mangosteen peels (Garcinia mangostana L.) is well known as an excellent source of antioxidative compounds. The name of mangosteen is xanthone. Antioxidant of mangosteen peels can be extracted by ethanol, etyl acetate and can be fractinated by etyl acetate and n-hexane. The aim of this research was to compare the antioxidant activity of the peel extract by ethanol and etyl acetate and fractinated by etyl acetate and n-hexane. Extract and fraction exhibited higher scavenging activity of DPPH. </span><span lang="EN">The purpose of this study was to compare antioxidant acti
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3

Ujjwal Kumar Mondol and W. Islam. "Bioseparation of phytochemical constituents from leaf and stem extracts of Mimosa pudica L." International Journal of Biological and Pharmaceutical Sciences Archive 6, no. 1 (2023): 023–32. http://dx.doi.org/10.53771/ijbpsa.2023.6.1.0075.

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The isolation procedure of chemical constituents was mainly based on fractionation by solvents of varying polarity. After cold extraction, solvent-solvent partitioning of extract was done with different solvents to yield different extracts of leaf and stem of Mimosa pudica L. Gas Chromatography-Mass Spectrometry (GC-MS) analysis was carried out for petroleum ether, chloroform and ethyl acetate extracts of leaf as well as chloroform and ethyl acetate extracts of stem and 16, 11, 3, 26 and 3 compounds were identified respectively. The major compounds were benzene,1-ethyl-3-methyl- (14.83%) for p
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4

Rohman, Abdul, and Sugeng Riyanto. "Aktivitas Antioksidan Ekstrak Buah Mengkudu (Morinda citrifolia, L)." agriTECH 25, no. 3 (2017): 131. http://dx.doi.org/10.22146/agritech.13347.

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This research aimed to determine antioxidant activity of Morinda citrifolia, L fruit extracts. Morinda citrifolia, L fruit was ground, extracted by methanol, and partitioned by using chloroform and ethyl acetate to recover extracts of methanol, chloroform and ethyl acetate respectively. These extracts were diluted to various concentrations i.e. 1, 5, and 10 %, and were determined their antioxidant activities by linoleic-thiocyanate method. The result showed that extracts of Morinda citrifolia, L fruit had antioxidant activity in the order of ethyl acetate extract > chloroform extract > m
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Ahmed, F., MB Meah, and F. Yasmin. "Isolation of Phomopsis Inhibitory Fraction of Allamanda Extract Removing Gum and Other Undesirable Compounds." Journal of Environmental Science and Natural Resources 5, no. 2 (2013): 199–203. http://dx.doi.org/10.3329/jesnr.v5i2.14814.

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Allamanda leaf extracts were made by three organic solvents hexane, methanol and ethyl acetate having different polarities. Thin layer chromatography (TLC) of the refluxing extracts showed each of them contained considerable number of different compounds. By observing Rf value of these extracts it was confirmed that the compounds present in different extracts are not same and two of them (methanol and ethyl acetate) extracts were distilled to remove solvent for bioactivity test. In growth inhibition test, methanol and ethyl acetate extracts of allamanda inhibited mycelial growth of Phomopsis v
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Duletić-Laušević, Sonja N., Aradski Ana Z. Alimpić, Stoimir M. Kolarević, Branka S. Vuković-Gačić, Mariana M. Oalđe, and Petar D. Marin. "Biological activities of Cretan Salvia pomifera extracts." Botanica Serbica 42, no. 2 (2018): 209–16. https://doi.org/10.5281/zenodo.1468304.

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The polyphenolic content and biological activities of dichloromethane, chloroform, ethyl acetate, and ethanol extracts of Cretan Salvia pomifera L. (Lamiaceae) were analysed. The ethyl acetate extract showed the highest content of total phenolics and total flavonoids. The ethanol extract exhibited the highest activity in the DPPH and FRAP assays, while the dichloromethane extract had the highest activity in the ABTS test. The ethyl acetate extract showed the highest activity in the β-carotene--linoleic acid system. The antioxidant activity of extracts was positively correlated with the to
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7

Srikanth, Racha, Murali Krishna Thupurani, Harish Rao B., and Challa Surekha. "IN VITRO AND IN VIVO ANTIDIABETIC EFFECTS OF SALACIA OBLONGA WALL LEAF AND ROOT EXTRACTS." International Journal of Advanced Research 10, no. 03 (2022): 841–49. http://dx.doi.org/10.21474/ijar01/14459.

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The present study was framed out to evaluate the antidiabetic effects of S. oblonga Wall leaf and root ethyl acetate and toluene extracts in vitro and in vivo. The root ethyl acetate extract found significant inhibition of alpha amylase and alpha glucosidase enzyme functions in vitro. The in vivo studies are carried out according to the guide line of CPCSEA and IAEC. The acute toxicity test of the extracts resulted in the no effect on the animals. The ethyl acetate extract of root showed the increase in body weight of animals at the end of the experiment. The blood glucose levels of diabetic r
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8

Ali, Wassen, and Mohammed R.Annon. "Biological Effective of organic solvent extracts of Mirabilis jalapa Leaves in the Non-cumulative for mortality of Immature stages Culex quinquefasciatus Say( Diptera : Culicidae )." Al-Qadisiyah Journal Of Pure Science 25, no. 1 (2020): 1–6. http://dx.doi.org/10.29350/jops.2020.25.1.1065.

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The present research evaluated the efficacy of organic solvent extracts (hexane, ethyl acetate, ethyl alcohol) separately using ppm
 (0,100,250, 500,1500, 1000, 2000) concentrations of Mirabilis jalapa leaves in the non-cumulative moetality of immature stages
 of Culex quinquefaciatus then estimate LC50 and LC90 by probit analysis. The hexane extract of the leaves M. jalapa of plant
 was the most affected in the non-cumulative mortality from that of ethyl acetate and ethyl alcohol. in the non-cumulative mortality
 of the immature stage . Where LC50 for egg mortality was ppm
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9

Wang, Jing, Jian Li, and Shu Jun Li. "Components of Cunninghamia lanceolata Heartwood Extracts." Materials Science Forum 685 (June 2011): 188–94. http://dx.doi.org/10.4028/www.scientific.net/msf.685.188.

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China-fir (Cunninghamia lanceolata) has a reputation for producing naturally durable heartwood. This species is native in China and has been extensive planted there. Mainly, extractives are responsible for the wood resistance to decay fungi. Compared with the number of studies on China fir focused only on essential oil, studies on other China fir wood extracts is few. In this report, different Cunninghamia lanceolata heartwood extracts were studied. Ground wood was sequentially extracted by solvents with different polarities, hexane, ethyl acetate and methanol. The components of the extracts w
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10

Kumar, T., A. Sahu, and V. Jain. "IN VITRO SCREENING OF LANNEA COROMANDELICA FOR ANTIFILARIAL ACTIVITY ON CATTLE FILARIAL PARASITE SETARIA CERVI." INDIAN DRUGS 51, no. 08 (2014): 28–34. http://dx.doi.org/10.53879/id.51.08.10112.

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The current study was aimed to investigate the antifilarial activity of aqueous and ethyl acetate extracts of the leaves of Lannea coromandelica. Both extracts were screened for their total flavonoid content chromatographically. The total amount of flavonoid present in ethyl acetate is much greater than in aqueous extract. Aqueous extract produced initial stimulation effect in whole worm followed by reversible paralysis, whereas on the nerve muscle preparation, the effect was depressant. In the same manner ethyl acetate extract produces irreversible paralysis. The concentrations required to in
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11

Nabilah, Almurdani M., Rudi Hendra, and Hilwan Yuda Teruna. "Antioxidant activities of medang lendir (Litsea glutinosa) stem bark." Pharmacy Education 23, no. 2 (2023): 122–25. http://dx.doi.org/10.46542/pe.2023.232.122125.

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Background: Medang lendir (Litsea glutinosa), a member of the Lauraceae family, is typically found in the Kampar Regency area of Riau Province, Indonesia. This plant is frequently used in traditional medicine to treat rheumatism, diarrhea, and dysentery. Objective: To evaluate the antioxidant activities of L. glutinosa stem bark extracts. Method: L. glutinosa stem bark was macerated in methanol to obtain methanol extract, followed by partitioning with ethyl acetate to obtain ethyl acetate extract. The antioxidant activities of these extracts were determined using the NO radical scavenging and
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12

Peraman, Manimegalai, and Selvam Kuppusamy. "Qualitative and quantitative phytochemical composition and antimicrobial activity of different solvent extracts of Hardwickia binata Roxb." Research Journal of Biotechnology 19, no. 3 (2024): 31–38. http://dx.doi.org/10.25303/1903rjbt031038.

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Hexane and ethyl acetate extracts were employed to carry out the research on the phytochemical and antibacterial activity of Hardwickia binata Roxb. To identify the components, present in the extract, the GC-MS technique was used for analysis. Functional groups of various extracts were identified using FTIR. The plant extract in hexane and ethyl acetate was evaluated for its ability to fight human pathogenic bacteria. The results of the phytochemical screening revealed that phenols, saponins, flavonoids, cardiac glycosides, steroids and tannins are present. The GC-MS data revealed that the eth
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13

Onyegbule, Felix A., Chukwudozie I. Okoye, Chidimma R. Chukwunwejim, Blessing O. Umeokoli, and Peter M. Eze. "Evaluation of Antioxidant, Anti-inflammatory and Antimicrobial Activities of the Leaf Extracts of Luffa cylindrica." Journal of Health Sciences 8, no. 2 (2018): 101–9. http://dx.doi.org/10.17532/jhsci.2018.423.

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Introduction: Luffa cylindrica have been used traditionally in the treatment and management of several disease conditions. This study aimed to evaluate the antioxidant, anti-inflammatory and antimicrobial properties of leaf extracts of the plant, and identifying some of its phytoconstituents.
 Methods: The crude ethanol and ethyl acetate extracts were evaluated for antioxidant and anti-inflammatory activities using the 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) and paw-fluid displacement methods, respectively. The extracts were tested for antimicrobial activity using the agar well diffusion a
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14

Simanjuntak, Vino Soaduon Hamonangan, Endah Wydiastuti, Lina Marlina, Partomouan Simanjuntak, and Rudi Kartika. "Pengujian Toksisitas dan Antioksidan Ekstrak Etilasetat dan n-Butanol Hipokotil Sarang Semut, Myrmecodia pendens Merr. & Perry." JURNAL KIMIA MULAWARMAN 21, no. 1 (2023): 55. http://dx.doi.org/10.30872/jkm.v21i1.1288.

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Sarang semut, Myrmecodia pendens Merr. & Perry (Rubiaceae) is an epiphytic plant from Papua which is used to cure various diseases. The purpose of this study is to see the chromatogram profile [Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC)] analysis, and biological test of ethyl acetate and n-butanol of Sarang semut’s hypocotyl extracts from methanol extracts. Meanwhile, the biological tests carried out were toxicity tests on Artemia salina L shrimp larvae and antioxidant assay with free radical scavenging methods (DPPH). The results of the antioxidant a
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15

Wassen Ali Adday and Mohammed Ridha Annon. "Biological Effective of organic solvent extracts of Datura innoxia Leaves in the Cumulative and Non-cumulative for mortality of Immature insect Culex quinquefasciatus Say (Diptera : Culicidae)." International Journal of Research in Pharmaceutical Sciences 11, no. 1 (2020): 452–59. http://dx.doi.org/10.26452/ijrps.v11i1.1841.

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The present research included the evaluation of the efficiency of organic solvent extracts (hexane, ethyl acetate, ethyl alcohol) separately using ppm (100, 250, 5001500, 1000, 2000) concentrations of Datura innoxia leaves. In the non- cumulative and cumulative mortality of the immature stage of Culex quinquefaciatus Say mosquitoes where LC50 and LC90 by probit analysis were estimated. The hexane extract of the leaves D.innoxia plant was the most affected hexane extract was the most effective of ethyl acetate and ethyl alcohol in the non-cumulative mortality of the immature instar where LC50 f
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16

Zaitun Hasibuan, Poppy Anjelisa, and Mardiana. "Antioxidant Activity of n-Hexane, Ethyl Acetate and Ethanol Extract from Lakoocha Leaves (Artocarpus lacucha Buch.-Ham) using DPPH Method." Indonesian Journal of Pharmaceutical and Clinical Research 1, no. 2 (2018): 41–47. http://dx.doi.org/10.32734/idjpcr.v1i2.433.

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This study aimed to investigate phytochemical screening and antioxidant activity of n–hexane, ethyl acetate and ethanol extract from lakoocha leaves. The powdered simplicia was macerated with n–hexane, ethyl acetate and ethanol 96% successively, filtered, then concentrated using rotary evaporator to obtain n–hexane extract, ethyl acetate extract and ethanol extract. Phytochemical screening and antioxidant activity was performed against these extracts. Antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging method using ultraviolet-visible spectrophotomete
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17

Praptiwi, Praptiwi, and Ahmad Fathoni. "PHYTOCHEMICAL, GAS CHROMATOGRAPHY-MASS SPECTROMETRY PROFILING AND FREE RADICAL SCAVENGING ACTIVITY OF EXOCARPOS LONGIFOLIUS (SANTALACEAE) EXTRACTS." Asian Journal of Pharmaceutical and Clinical Research 11, no. 10 (2018): 452. http://dx.doi.org/10.22159/ajpcr.2018.v11i10.25926.

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Objective: The objectives of the study were to investigate phytochemical screening and gas chromatography-mass spectrometry (GC-MS) profiling of twigs and leaves of Exocarpos longifolius and its 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity.Methods: Phytochemical screening covering saponins, alkaloids, terpenoids, flavonoids, and tannins were carried out by standard methods. The free radical scavenging activity was performed by thin-layer chromatography - bioautography, followed by determining the IC50 values of active extracts. Chemical compounds of active extract were examin
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18

Islam, Mohammad Emdadul, Tofail Ahmed Chowdhury, Nasim Sultana, Abul Hasnat Muhammad Solaiman, and SM Anamul Arefin. "Biological activity studies of Sensevieria hyacinthoides extracts." Asian-Australasian Journal of Bioscience and Biotechnology 5, no. 1 (2020): 15–20. http://dx.doi.org/10.3329/aajbb.v5i1.53857.

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Biological activity was studied of the different solvent extracts such as n-hexane, ethyl acetate, n-butanol, and methanol extracts of the rhizomes of Sensevieria hyacinthoides. The cytotoxic potential were examined by using brine shrimp lethality bioassay. Ethyl acetate extract exhibited quite potent activity in brine shrimp lethality bioassay with LC50 1.95 μg/mL, respectively. These result suggested that it might contain antitumor or cytotoxic agent. The ethyl acetate extract showed significant free radical scavenging activity with IC50 10.51 μg/mL and demonstrated excellent antibacterial a
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19

Achuenu, Chukuka, Emesi Ijeoma Chinenye, Raymond Dashe, Edah Alexander O, and Suleman Stephen M. "Investigation of Total Phenolic and Flavonoid Content Present in the Leaf and Stalk Extracts of Ocimum Gratissimum (Scent Leaf) Cultivated In Jos, Nigeria." International Journal of Research and Scientific Innovation XII, no. I (2025): 547–52. https://doi.org/10.51244/ijrsi.2025.12010049.

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The present study examines the total phenolic and flavonoid content in the leaf and stalk of Ocimum gratissimum grown in Jos by using the maceration method for extraction. Ethanol and ethyl acetate were used as solvents for the extraction of bioactive compounds from the plant material. Total phenolic content (TPC) of the extracts was determined as Gallic Acid Equivalent (GAE). The results showed that the TPC of the ethyl acetate extract was significantly higher (231.78 ± 2.3609 mg/100g GAE) than that obtained from ethanol extract (182.61 ± 0.7765 mg/100g GAE). Ethyl acetate, hence, proved to b
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20

Santoso, Bimo Budi, Argina Argina, and Alfhons D. Sirampun. "AKTIVITAS ANTIBAKTERI DAN KOMPONEN KIMIA EKSTRAK HEKSAN, ETIL ASETAT DAN ETANOL BATANG BROTOWALI (Tinospora crispa Linn) ASAL MANOKWARI." Jurnal Natural 16, no. 2 (2020): 112–19. http://dx.doi.org/10.30862/jn.v16i2.115.

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Antibacterial activity test and chemical component analysis of hexane, ethyl acetate and ethanol extract of Brotowali stem from Manokwari Regency against B. subtillis and E. coli have been carried out. The extraction of the T. crispa stems was carried out by the maceration method in stages based on the polarity of the solvent. Chemical component analysis was performed using GC-MS and phytochemical tests. Based on the identification of chemical compounds by phytochemical screening, it shows the presence of flavonoids in the hexane extract, while in the ethyl acetate and ethanol extracts there a
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Erida, G., Hasanuddin, A. Marliah, and Zulwanis. "Herbicidal activity of n-hexane, ethyl acetate and methanol purple nutsedge (Cyperus rotundus L.) extracts on Amaranthus spinosus L." IOP Conference Series: Earth and Environmental Science 1297, no. 1 (2024): 012032. http://dx.doi.org/10.1088/1755-1315/1297/1/012032.

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Abstract Environmentally friendly weed control is highly recommended to be implemented in agriculture. The objective of this research was to assess the herbicidal efficacy of n-hexane, ethyl acetate, and methanol extracts derived from the tuber of Cyperus rotundus L. on Amaranthus spinosus L. The tuber was consecutively extricated with n-hexane, ethyl acetate, and methanol. These extracts were given to A. spinosus in pot culture at concentrations of 5%, 10%, 15%, and 20%.The herbicide (2,4-D at 0.686 kg a.i. ha−1) and distilled water were acted as positive and negative controls. The extract ha
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22

Mansur, Muhammad Abdullah Al, M. Mahboob Ali Siddiqi, and Koushik Saha. "Thrombolytic, Membrane Stabilizing and Hypoglycemic Activities of Anethum sowa Linn." Dhaka University Journal of Pharmaceutical Sciences 17, no. 2 (2018): 237–41. http://dx.doi.org/10.3329/dujps.v17i2.39181.

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The hexane (HE), dichloromethane (DCME), ethyl acetate (EAE) and methanol (ME) extracts of seed and stem of Anethum sowa were subjected to screenings for thrombolytic, membrane stabilizing and hypoglycemic activities. Ethyl acetate extract of stem showed highest thrombolytic activity. In addition, the dichloromethane, ethyl acetate and methanol extract of seed revealed higher percentage (%) of inhibition in hypotonic solution induced hemolysis. In hypoglycemic activity, the dichloromethane and ethyl acetate extracts of stem and seed displayed significant blood glucose lowering effect.
 Dh
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23

Awotedu BF, Omolola TO, Akala AO, Ogunsiji AO, Owoeye EA, and Olaoti-Laaro SO. "Radical Scavenging activity and phytochemical screening of the leaf extract of Synsepalum dulcificum (Schumach. & Thonn.) Daniell." World Journal of Advanced Research and Reviews 10, no. 3 (2021): 069–76. http://dx.doi.org/10.30574/wjarr.2021.10.3.0241.

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Antioxidants are chemicals that possess bioactive constituents which usually allow them to fight against free radicals. Antioxidant and phytochemicals properties of the leaves of Synsepalum dulcificum were investigated using ethyl-acetate, ethanol and methanol extracts. 2, 2-diphenyl-picrylhydrazyl (DPPH) scavenging activity, total antioxidant activity, ferric reducing antioxidant power (FRAP), total flavonoid and the total phenolic content was carried out. The phytochemical screening assay was done on the following: Alkaloids, saponin, flavonoids, tannin, anthraquinones, terpenoids, cardiac g
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Lenny, Sovia, Murni Suryani Lumbantoruan, and Helmina Br Sembiring. "Antioxidant Activity Assay of Eucalyptus pellita Leaf Extract." International Journal of Ecophysiology 5, no. 2 (2023): 26–33. http://dx.doi.org/10.32734/ijoep.v5i2.13715.

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In pharmacy, eucalyptus leaves are used for various pharmacological activities because they contain phenolic compounds. This study aims to detect phytochemical compounds and test the antioxidant activity of Eucalyptus pellita leaf extract. The dry powder of E. pellita leaves (1900 g) was macerated first with methanol solvent. The concentrated methanol extract was then subjected to partition extraction with n - hexane solvent. The methanol layer was concentrated and then partitioned with ethyl acetate solvent. Methanol extract was obtained as much as 176.75 g (9,30%), n-hexane extract 13.77 g (
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Niaci, Sarah. "PHYTOCHEMICAL PROPERTIES OF METHANOL AND ETHYL ACETATE EXTRACTS OF CANANGA ODORATA FLOWERS AND THEIR PHARMACOLOGICAL ACTIVITIES." Journal of Medical pharmaceutical and allied sciences 10, no. 6 (2021): 3874–77. http://dx.doi.org/10.22270/jmpas.v10i6.1608.

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This work aimed to investigate the phytochemical contents of methanol as well as ethyl acetate extracts from Cananga odorata flower and their activities against a skin acne bacterium - Propionibacterium acnes. Herein, methanol extract of C. odorata was sequentially partitioned using nhexane and ethyl acetate as solvents. Each extract was studied for its antibacterial activity against P. acnes bacteria. Antibacterial testing was conducted based on disc diffusion assay technique, where the discs were dripped with several variants of concentrations of C. odorata flower extract ranging from 0.5, 2
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Ickovski, Jovana D., Ivan R. Palić, Aleksandra S. Đorđević, Vesna P. Stankov Jovanović, Violeta D. Mitić, and Gordana S. Stojanović. "Total phenolic content, antioxidant capacity, and antimicrobial activity of Origanum heracleoticum L., extracted with different solvents." Chemia Naissensis 5, no. 2 (2022): 36–49. http://dx.doi.org/10.46793/chemn5.2.36i.

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This research was based on the examination of the total phenolic content, antioxidant and antimicrobial activities of hexane, diethyl ether, ethyl acetate, and methanol extracts of Origanum heracleoticum L. grown in Serbia. The antimicrobial activity was determined against five bacteria and two fungi using the disk diffusion method. The total phenolic content of O. heracleoticum solvent extracts was determined and five different tests were used for screening of the antioxidant capacity. The highest total phenolic content was found in ethyl acetate extract (848.48 μg GAE/mg dry extract) and met
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27

IBRAHIM, Abeer Y., Souad E. El-GENGAIHI, Hemaia M. MOTAWEA, and Amany A. SLEEM. "Anti-Inflammatory Activity of Salvadora persica L. against Carrageenan Induced Paw Oedema in Rat Relevant to Inflammatory Cytokines." Notulae Scientia Biologicae 3, no. 4 (2011): 22–28. http://dx.doi.org/10.15835/nsb346378.

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In this study, the anti-inflammatory effect of aqueous alcoholic crude extract and ethyl acetate extract of miswak sticks (Salvadora persica L.) was investigated in carrageenan induced rat paw oedema in respect to immunological parameters. Adult male sapargue dawely rats were classified into four groups, group I received the vehicle (0.25% gum acacia solution), group II received crude aqueous alcoholic extract orally at 100 mg/kg, group III received ethyl acetate extract (100 mg/kg) orally and group IV received indomethacin (20 mg/kg) orally, and served as standard reference. The oedema was qu
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Boonkusol, Duangjai, Suchada Sawasdee, Janejira Detraksa, Kansuda Duangsrikaew, and Prachart Watcharabundit. "Evaluation of Anti-Escherichia coli K88 from Diarrhea Piglets and Phytochemicals of Galangal Rhizome Extracts." Trends in Sciences 21, no. 1 (2023): 7112. http://dx.doi.org/10.48048/tis.2024.7112.

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This study investigated the antimicrobial effect of galangal rhizome extracts against Escherichia coli K88 from diarrhea pigs. Galangal rhizome was extracted with 4 solvents: Water, ethanol, methanol and ethyl acetate. The extracts determination against E. coli K88 by disc diffusion method, minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). Results showed that inhibition diameters of galangal extract with ethyl acetate against E. coli K88 was significantly higher than that of galangal extracted with water, ethanol and methanol. However, the inhibition was sign
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29

M. M., Nisha, S. Santhosh Kumar, N. Ibomcha, S. Susmitha, and B. V. Muddukrishna. "In Vitro Study on Actinomycetes Extracts Against The Stem Bleeding Disease of Coconut." CORD 38 (March 21, 2022): 1–7. http://dx.doi.org/10.37833/cord.v38i.431.

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Several compounds produced by Actinomycetes group of bacteria have proven potential in inhibiting plant pathogenic fungi. In the present study, two microbial ethyl acetate extracts from Streptomyces were tested for their antifungal properties against Thielaviopsis paradoxa, the causal organism of stem bleeding disease in coconut. The cultural filtrates of 2 strains of Streptomyces (AFL-1 and AFL-2) were extracted with Ethyl acetrate. The resulting ethyl acetate extract of AFL-1 showed complete in-vitro inhibition of fungal growth at relatively low concentrations (0.25%) while Ethyl ecetate ext
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Saravanan, Renuka, Brindha Pemaiah, Mahesh Narayanan, and Sivakumar Ramalingam. "GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS, IN VITRO CYTOTOXIC AND ANTIOXIDANT EFFICACY STUDIES ON CLEOME GYNANDRA L. (LEAVES): A TRADITIONAL DRUG SOURCE." Asian Journal of Pharmaceutical and Clinical Research 10, no. 10 (2017): 84. http://dx.doi.org/10.22159/ajpcr.2017.v10i10.19343.

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Objective: This study was aimed to assess the phytoconstituents, cytotoxic, and antioxidant efficacy of ethyl extract of Cleome gynandra leaves.Methods: Qualitative phytochemical analysis with different solvent extracts was performed. Quantitative and gas chromatography-mass spectrometry (GC-MS) analysis of the extract was performed with ethyl acetate extract. The cytotoxic effect of the ethyl acetate extract was determined by 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide (MTT) assay on Michigan Cancer Foundation-7 (MCF-7) cells using taxol as standard and free radical scavenging
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Cardona-G, Wilson, Sara Robledo, Fernando Alzate, et al. "Antileishmanial and cytotoxic activities of four Andean plant extracts from Colombia." October-2020 13, no. 10 (2020): 2178–82. http://dx.doi.org/10.14202/vetworld.2020.2178-2182.

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Background and Aim: Licania salicifolia (L.S) Cuatrec., Persea ferruginea (P.F) Kunth, Oreopanax floribundus (O.F), and Psychotria buchtienii (P.B) belong to the families Chrysobalanaceae, Lauraceae, Araliaceae, and Rubiaceae, respectively, which have been used as medicines by communities in the Andes. This study evaluated the leishmanicidal and cytotoxic activities of alcohol and non-alcohol extracts from four Andean plant extracts (L.S, O.F, P.F, and P.B). Materials and Methods: Extracts were obtained by percolation with solvents of different polarities – hexane, dichloromethane, ethyl aceta
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Dai, Trang Thi Xuan, and Anh Thi Tu Vo. "Antioxidant and antimicrobial activities against Enterobacter cloacae of extracts of Eclipta alba Hassk." Science and Technology Development Journal 19, no. 4 (2016): 76–83. http://dx.doi.org/10.32508/stdj.v19i4.599.

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This study was subjected to investigate antimicrobial and antioxidant properties of Eclipta alba extracts (methanol, hexane, chloroform and ethyl acetate extracts). The antioxidant property of the extract was assessed by 2,2- diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. Kirby-Bauer method was used to determine the antibacterial activity against Enterobacter cloacae of the extracts. The extracts of Eclipta alba were tested against E. cloacae. The highest antibacterial potentiality was exhibited by the ethyl acetate extract of E. alba leaves, means of zones of bacterial growt
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Jebashree, H. Shyla, S. Jayasurya Kingsley, Emmanuel S. Sathish, and D. Devapriya. "Antimicrobial Activity of Few Medicinal Plants against Clinically Isolated Human Cariogenic Pathogens—An In Vitro Study." ISRN Dentistry 2011 (June 8, 2011): 1–6. http://dx.doi.org/10.5402/2011/541421.

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Hexane, ethyl acetate, ethanol and methanol extracts of Psidium guajava, Terminalia chebula, Mimusops elengi and Achyranthes aspera were tested against the dental caries causing bacteria Streptococcus mutans and fungus Candida albicans isolated from caries infected patients. All the four extracts of P. guajava showed activity against both S. mutans and C. albicans. Maximum zone of inhibition was observed in ethyl acetate of P. guajava. The four extracts of T. chebula and M. elengi showed antibacterial activity against S. mutans. M. elengi extracts and ethanol extract of T. chebula did not show
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Imran, Nurlian, L. A. Kadir, La Agusu, and Ruslan. "Penyarian Konstituen Organik Daging Buah Gaharu (Aquilaria malaccensis Lamk.) dengan Metode Maserasi Berbantu Microwave dan Uji Aktivitas Sebagai Antioksidan." KOVALEN: Jurnal Riset Kimia 7, no. 1 (2021): 42–50. http://dx.doi.org/10.22487/kovalen.2021.v7.i1.15478.

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This study was conducted to determine the organic constituents and to test the antioxidant activity and toxicity of gaharu fruit pulp extract (Aquilaria malaccensis Lamk.). Gaharu fruit pulp extract was obtained through Microwave Assisted Extraction (MAE) method and then extracted with a solvent with different polarity, namely ethyl acetate and n-hexane. The obtained yield of ethyl acetate extract of the gaharu fruit pulp was 40.827% w/v, whereas 0% w/v in n-hexane extract, therefore, no further test for n-hexane extract, so that the n-hexane extract was not tested for the next stage. The resu
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Ofusori, Adebimpe Esther, Yanuardi Raharjo, David Adesanya Ofusori, and Victory Opeyemi Adekunle. "A Comparative Study of Dichloromethane and Ethyl Acetate Root Extracts of <i>Celosia trigyna</i>: Phytochemical and Wound Healing Effect Analyses." Journal of Wound Management and Research 19, no. 2 (2023): 87–95. http://dx.doi.org/10.22467/jwmr.2023.02411.

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Background: &lt;i&gt;Celosia&lt;/i&gt; species have been validated to possess antimicrobial, anti-inflammatory, and antioxidant potential. This study was thus designed to comparatively determine the effect of dichloromethane and ethyl acetate root extracts of &lt;i&gt;Celosia trigyna&lt;/i&gt; on Wistar rat wounds created by 8 mm biopsy punches.Methods: Phytochemical screening was performed on dichloromethane and ethyl acetate root extracts of &lt;i&gt;C. trigyna&lt;/i&gt;. For animal studies, 25 adult female Wistar rats were randomly divided into five groups (group A-E; n=5). Group A served a
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Plesa, Carmen Manuela, Aurel Ardelean, Mihai Cosmin Pascariu, and Alfa Xenia Lupea. "Comparative Antioxidant Properties of Juniperus communis L. and Juniperus virginiana L. Extracts Obtained by Refluxing and Sonication Methods." Revista de Chimie 68, no. 5 (2017): 899–902. http://dx.doi.org/10.37358/rc.17.5.5576.

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The aim of this study was to evaluate the antioxidant activity of ethyl acetate and tetrahydrofuran extracts of Juniperus berries, branches and needles, by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Two Juniperus species, Juniperus communis and Juniperus virginiana, from different regions were used. The extracts obtained by sonication and refluxing extraction were studied by gas chromatography-mass spectrometry, which revealed that the Romanian juniper extracts are largely comprised of monoterpene hydrocarbons, such as a-pinene, b-pinene, b-hellandrene, sabinene, b-cadinene, t-cadin
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Hepsibah, A. Hannah, M. Mala, and G. Jeya Jothi. "ANTIMICROBIAL ACTIVITY AND TLC PROFILING OF CLERODENDRUM PHLOMIDIS LINN. F LEAF EXTRACT AGAINST MULTI-DRUG RESISTANT CLINICAL PATHOGENS." International Journal of Pharmacy and Pharmaceutical Sciences 9, no. 9 (2017): 275. http://dx.doi.org/10.22159/ijpps.2017v9i9.20186.

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Objective: The aim of the present study is to evaluate the antibacterial and antifungal activities of different solvent extracts of leaves of Clerodendrum phlomidis against multi-drug resistant clinical pathogens and to perform Thin Layer Chromatography (TLC) profiling of the extracts.Methods: The leaf powder was sequentially extracted with petroleum ether, ethyl acetate, acetone, and methanol. The extracts were tested for their antimicrobial activity against 20 bacterial strains and 15 fungal strains which were multiple drugs resistant by disc diffusion method. TLC profiling of all four extra
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Humairani, Rindhira, Nanda Rizki Purnama, Herpandi Herpandi, et al. "Cytotoxicity of leaves, stems, and flowers of Kecubung (Datura metel) extracts using the Brine Shrimp Lethality Test (BSLT) method." Depik 12, no. 2 (2023): 117–24. http://dx.doi.org/10.13170/depik.12.2.27445.

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Brine shrimp (Artemia salina) cytotoxicity assays (BSLT) are one of the commonly used cytotoxic test methods to assess a plant extract's pharmacological activity and toxicity. Thus, this study aimed to examine the toxic levels of Kecubung (Datura metel) leaves extracted using different solvents, namely ethanol and ethyl acetate. The results showed that the LC50 value of flower ethanol, flower ethyl acetate, leaf ethanol, and stem ethyl acetate extracts had an LC50 value 1000, which was included in the toxic category. The LC50 value of flower ethanol is 121.044 ppm, flower ethyl acetate 105.89
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Sijabat, Yosi Yananda, Wilis Ari Setyati, and Retno Hartati. "Phytochemical Screening and Toxicity Test of Various Extracts from Microalgae <I>Dunaliella salina</I>." Jurnal Ilmiah Perikanan dan Kelautan 15, no. 1 (2023): 179–88. http://dx.doi.org/10.20473/jipk.v15i1.36282.

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Highlight Research Extract N-hexane of salina contains alkaloids, steroids, triterpenoids, and phenols Extract Ethyl Acetate of salina contains alkaloids, steroids, triterpenoids, phenols, flavonoid, and saponins Extract Methanol of salina contains alkaloids, steroids, triterpenoids, phenols, flavonoid, and saponins 24- hours LC50 value of the n-hexane extract salina was 276 ppm, the ethyl acetate extract was 673 ppm and the methanol extract was 811 ppm. All of the three extracts were included in toxic category Abstract Microalgae are single celled microorganisms as the primary producers in th
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Regis, Anto Suganya, and Jeya Jothi Gabriel. "ANTIFUNGAL ACTIVITY OF CYANOTIS AXILLARIS (L.) D. DON EX SWEET AGAINST OPPORTUNISTIC FUNGAL STRAINS." International Journal of Pharmacy and Pharmaceutical Sciences 9, no. 7 (2017): 140. http://dx.doi.org/10.22159/ijpps.2017v9i7.18868.

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Objective: The main focus of the study was to compare and determine the antifungal activities of different solvent extracts of Cyanotis axillaris.Methods: The dried whole plant of C. axillaris was extracted by sequential extraction method using solvents hexane, ethyl acetate and methanol based on their polarity. The antifungal activity of the extracts was tested against 12 opportunistic fungal strains by disc diffusion method. Minimum inhibitory concentration (MIC) was determined using microtiter plate method.Results: The hexane, ethyl acetate and methanol extracts showed significant antifunga
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Haider M. Badea Albadri, Ibrahim Saleh Al-Juboori, and Zainab Yaseen Mohammed Hasan. "Antihypertrophic Scar Effect of Iraqi Plantago major Extracts." Al Mustansiriyah Journal of Pharmaceutical Sciences 24, no. 3 (2024): 320–29. http://dx.doi.org/10.32947/ajps.v24i3.1068.

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Background: Plantago major, historically renowned for its medicinal attributes across diverse cultures, has recently been under the research spotlight for its antihypertrophic scar effects. Aim: To investigate the efficacy of Plantago major extracts in the treatment of hypertrophic scars, particularly comparing the effects of methanol and ethyl acetate extracts. Methodology: Samples of the plant, procured from Baghdad's Al-Salihiya Neighbourhood in November 2021, underwent authentication at the Iraqi local Herbarium in the Al-Razi centre for alternative medicine. Using the Soxhlet apparatus, t
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Agossou Esse, Akotegnon Rodrigue, Savoeda Perside, Behanzin G. Justin, and Sezan Alphonse. "Evaluation of the antiproliferative activity of three types of Annona muricata extract in the traditional treatment of cervical cancer induced in Wistar rats." International Journal of Science and Research Archive 9, no. 1 (2023): 308–17. http://dx.doi.org/10.30574/ijsra.2023.9.1.0170.

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This study was concerned with the evaluation of the antiproliferative activity of Annona muricata in the traditional treatment of cervical cancer induced in Wistar rats. The aim of the present work was to compare the effect of ethyl, dichloromethane and ethyl acetate extracts of Annona muricata leaves to determine the most effective one on cervical cell differentiation in wistar rats. For this purpose, we made three extracts namely: ethyl extract, dichloromethane and ethyl acetate) from dried leaf powder of Annona muricata. The antiproliferative (anticancer) activity was evaluated by assaying
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Awotedu, BF, TO Omolola, AO Akala, AO Ogunsiji, EA Owoeye, and SO Olaoti-Laaro. "Radical Scavenging activity and phytochemical screening of the leaf extract of Synsepalum dulcificum (Schumach. & Thonn.) Daniell." World Journal of Advanced Research and Reviews 10, no. 3 (2021): 069–76. https://doi.org/10.5281/zenodo.5066451.

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Antioxidants are chemicals that possess bioactive constituents which usually allow them to fight against free radicals. Antioxidant and phytochemicals properties of the leaves of&nbsp;<em>Synsepalum dulcificum</em>&nbsp;were investigated using ethyl-acetate, ethanol and methanol extracts. 2, 2-diphenyl-picrylhydrazyl (DPPH) scavenging activity, total antioxidant activity, ferric reducing antioxidant power (FRAP), total flavonoid and the total phenolic content was carried out. The phytochemical screening assay was done on the following: Alkaloids, saponin, flavonoids, tannin, anthraquinones, te
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Rasmy, Soad, Gulsen Saleh, Enayat Hassan, Eman Negm, and Walaa Amin. "Antioxidant And Anti-Mycotoxin Activities, And Cytotoxicity Properties In Vitro Of Propolis Extracts." Journal of Advanced Zoology 44, no. 4 (2023): 374–83. http://dx.doi.org/10.17762/jaz.v44i4.1291.

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Abstract: The present study aims to determine the antioxidant activity and cytotoxic activity of propolis and evaluate the anti-mycotoxin activity of different propolis extracts. The three extracts of propolis (ethyl acetate, ethanol (75%), and water) were subjected to testing of the antioxidant potential, and total phenolic and flavonoid contents. determination of the cytotoxicity activity by standard cell culture method against colon and liver and the ability of propolis extracts to control aflatoxin (AF) production. The results revealed that the highest value of antioxidant activities was f
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Namoune, I., B. Khettal, A. M. Assaf, S. Elhayek, and L. Arrar. "Antioxidant and Anti-inflammatory Activities of Organic and Aqueous Extracts of Northeast Algerian Marrubium vulgare." Phytothérapie 16, S1 (2018): S119—S129. http://dx.doi.org/10.3166/phyto-2018-0106.

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Marrubium vulgare (Lamiaceae) is frequently used in traditional medicine to treat many illnesses from ancient times. Its beneficial effects include antibacterial, antioedematogenic, and analgesic activities. This study was designed to evaluate the antioxidant and anti-inflammatory activities of organic and aqueous extracts of the leaves, the flowers, the stems, and the roots of Marrubium vulgare. The total phenolic and flavonoid contents as well as the antioxidant and the anti-inflammatory effects of methanol, chloroform, ethyl acetate, and aqueous extracts have been investigated by using diff
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Oh, Junsang, Eunhyun Choi, Jayoung Kim, Heesu Kim, Sangheun Lee, and Gi-Ho Sung. "Efficacy of Ethyl Acetate Fraction of Cordyceps militaris for Cancer-Related Fatigue in Blood Biochemical and 1H-Nuclear Magnetic Resonance Metabolomic Analyses." Integrative Cancer Therapies 19 (January 2020): 153473542093263. http://dx.doi.org/10.1177/1534735420932635.

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This study investigated the adjuvant effects for anticancer and antifatigue of the combination of Cordyceps militaris extract with sorafenib. The 5 extracts of C militaris were obtained through hexane, chloroform, ethyl acetate, butanol, and water and were evaluated for anticancer growth activity. Among these extracts, ethyl acetate extract of C militaris showed the best tumor growth inhibitory activity and the adjuvant effects in combination with sorafenib. As a result of biochemical analysis with serum, the combination of ethyl acetate extract of C militaris with sorafenib showed the adjuvan
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., Megawati, Berna Elya, and Nuraini Puspitasari. "INHIBITION OF 3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE ACTIVITY BY EXTRACTS OF GARCINIA XANTHOCHYMUS MESOCARP AND TOTAL FLAVONOID ASSAY QUANTIFICATION OF THE MOST ACTIVE EXTRACT." International Journal of Applied Pharmaceutics 10, no. 1 (2018): 264. http://dx.doi.org/10.22159/ijap.2018.v10s1.59.

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Objective: This study aims to determine the inhibitory activity of Garcinia xanthochymus mesocarp extracts against 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase.Methods: G. xanthochymus mesocarp was macerated sequentially using n-hexane, ethyl acetate, and methanol. Phytochemical screening andquantification of total flavonoids were performed on the most active extract.Results: Based on the tests, n-hexane, ethyl acetate, and methanol extracts had inhibitory activities of 12.30±1.098%, 55.63±10.584%, and44.01±1.053%, respectively. The results showed that the ethyl acetate is the mos
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Talla, Emmanuel, Alfred Ngenge Tamfu, Pierre Biyanzi, et al. "Phytochemical screening, antioxidant activity, total polyphenols and flavonoids content of different extracts of propolis from Tekel (Ngaoundal, Adamawa region, Cameroon)." Journal of Phytopharmacology 3, no. 5 (2014): 321–29. http://dx.doi.org/10.31254/phyto.2014.3504.

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Five extracts of propolis of Adamawa Cameroon were obtained by percolation and maceration with five different solvents: hexane, ethyl acetate, ethanol, methanol and water, in order of increasing polarity. Phytochemical screening was carried out on the extracts and the total content in flavonoids and polyphenols were evaluated by photometric methods. The total flavonoid content was evaluated using the Neu reagent (2-aminodiethyl diphenylborinate) and quercetin as standard and the results varied from 0.84±0.02 gQE/100gRM in ethyl acetate extract to 1.52±0.06 gQE/100gRM in ethanol extract. The to
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Ratnaningtyas, Nuniek Ina, Purnomowati Purnomowati, Endang Sri Purwati, Aisyah Tri Septiana, Nuraeni Ekowati, and Adi Supriyadi. "Antioxidant Potential of Ethanol and Ethyl Acetat Extract of Ganoderma sp. Mycelium." Biosaintifika: Journal of Biology & Biology Education 10, no. 1 (2018): 87–94. http://dx.doi.org/10.15294/biosaintifika.v10i1.11512.

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Ganoderma sp. Banyumas 1 isolate that reffered as Ganoderma sp. is a new discovered isolate from Banyumas, Central Java, Indonesia expected to have a potential properties of antioxidant of medicinal mushroom. This study aimed to determine the antioxidant potential and the appropriate solvent for it’s extracting from Ganoderma sp. This research result showed that ethyl acetate was able to extract as many as 15.57%, while etanol was only able to extract 3.87% active compounds from dried 28 days old Ganoderma sp. mycelium cultivated in the Mushroom Complete Medium (MCM). Extract of ethyl acetate
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Nur, S., M. Hanafi, H. Setiawan, and B. Elya. "In vitro ultra violet (UV) protection of curculigo latifolia extract as a sunscreen candidate." IOP Conference Series: Earth and Environmental Science 1116, no. 1 (2022): 012009. http://dx.doi.org/10.1088/1755-1315/1116/1/012009.

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Abstract Curculigo latifolia is a local plant from the Amaryllidaceae tribe, which the community in empirical medicine has widely used. This study aims to determine the bioactivity of C. latifolia extract as a sunscreen carried out in vitro to obtain scientific data to be developed as a raw sunscreen material. The dried simplicia powder from the roots, stems, and leaves of C. latifolia was extracted by graded maceration using n-hexane, ethyl acetate, and ethanol (70% v/v) to obtain an extract. Each extract from plant parts was observed for the sunscreen profile in absorbing UVA (320-400 nm) an
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