To see the other types of publications on this topic, follow the link: Fluorescein forms.

Journal articles on the topic 'Fluorescein forms'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Fluorescein forms.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Surzhikova, D. P., M. A. Gerasimova, and E. A. Slyusareva. "Protolytic equilibrium of excited states of fluorescein." Izvestiya vysshikh uchebnykh zavedenii. Fizika, no. 11 (2021): 108–14. http://dx.doi.org/10.17223/00213411/64/11/108.

Full text
Abstract:
The protolytic equilibrium of the excited states of fluorescein depends not only on the equilibrium in the ground state, but also on the efficiency of photoexcitation, relaxation and proton transfer in the excited state. Due to the competing rate of radiative and nonradiative processes occurring in a mixture of protolytic forms, there is still a problem of identifying the fluorescent characteristics of individual forms of fluorescein. In this work, the stationary distribution of the concentrations of the ground and excited states of the cation, a number of tautomers of the neutral form, monoan
APA, Harvard, Vancouver, ISO, and other styles
2

Burilov, Vladimir, Aigul Fatykhova, Diana Mironova, et al. "Oxyethylated Fluoresceine—(thia)calix[4]arene Conjugates: Synthesis and Visible-Light Photoredox Catalysis in Water–Organic Media." Molecules 28, no. 1 (2022): 261. http://dx.doi.org/10.3390/molecules28010261.

Full text
Abstract:
Fluorescent derivatives attract the attention of researchers for their use as sensors, photocatalysts and for the creation of functional materials. In order to create amphiphilic fluorescent derivatives of calixarenes, a fluorescein derivative containing oligoethylene glycol and propargyl groups was obtained. The resulting fluorescein derivative was introduced into three different (thia)calix[4]arene azide derivatives. For all synthesized compounds, the luminescence quantum yields have been established in different solvents. Using UV-visible spectroscopy, dynamic light scattering, as well as t
APA, Harvard, Vancouver, ISO, and other styles
3

Shimomura, O., and A. Shimomura. "Halistaurin, phialidin and modified forms of aequorin as Ca2+ indicator in biological systems." Biochemical Journal 228, no. 3 (1985): 745–49. http://dx.doi.org/10.1042/bj2280745.

Full text
Abstract:
Two kinds of aequorin-type photoproteins, i.e., halistaurin and phialidin, and four kinds of modified forms of aequorin, i.e., products of acetylation, ethoxycarbonylation, fluorescamine-modification and fluorescein labelling, were prepared. The modified forms of aequorin were more sensitive to Ca2+ than was aequorin in their Ca2+-triggered luminescence reactions, whereas halistaurin and phialidin were less sensitive. The emission maxima of luminescence were all within a wavelength range 450-464 nm, except for fluorescein-labelled aequorin, which emitted yellowish light (lambda max. 520 nm). A
APA, Harvard, Vancouver, ISO, and other styles
4

Diehl, Harvey. "Studies on fluorescein—VI☆Absorbance of the various prototropic forms of yellow fluorescein in aqueous solution." Talanta 36, no. 3 (1989): 413–15. http://dx.doi.org/10.1016/0039-9140(89)80212-7.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Hsu, Ting-Chang, Kathleen P. Pratt, and Arthur R. Thompson. "The factor VIII C1 domain contributes to platelet binding." Blood 111, no. 1 (2008): 200–208. http://dx.doi.org/10.1182/blood-2007-01-068957.

Full text
Abstract:
Activated factor VIII (FVIIIa) forms a procoagulant complex with factor IXa on negatively charged membranes, including activated platelet surfaces. Membrane attachment involves the FVIII C2 domain; involvement of the adjacent C1 domain has not been established. Binding of recombinant FVIII C1C2 and C2 proteins to platelets was detected by flow cytometry using (1) anti-C2 monoclonal antibody ESH8 followed by a phycoerythrin-labeled secondary antibody; (2) biotinylated C1C2 detected by phycoerythrin-labeled streptavidin, and (3) C1C2 and C2 site-specifically labeled with fluorescein. Highest bin
APA, Harvard, Vancouver, ISO, and other styles
6

Shikanai-Yasuda, M. A., R. T. Taguchi, M. K. Sato, et al. "In vitro action of some disinfectants on Paracoccidioides brasiliensis yeast forms." Revista do Instituto de Medicina Tropical de São Paulo 33, no. 1 (1991): 37–43. http://dx.doi.org/10.1590/s0036-46651991000100008.

Full text
Abstract:
The fungicidal action of sodium hypochlorite (0.3, 1, 2.5, 5 and 10%); formaldehyde (2, 5, and 10%); and ethyl alcohol (70%) on yeast forms of Paracoccidioides brasiliensis Pb 18 and a newly-isolated Goiana strain was described. Contact between the fungus and the disinfectants was maintained for 1, 2, 24, 48 and 72 hours at room temperature. Viability was evaluated by the fluorescein diacetate-ethidium bromide treatment, culture in solid and liquid media (36ºC and 26ºC); yeast to mycelial germination at room temperature; and radiometric study of metabolic activity. All concentrations of disinf
APA, Harvard, Vancouver, ISO, and other styles
7

Klonis, Nectarios, and William H. Sawyer. "Spectral properties of the prototropic forms of fluorescein in aqueous solution." Journal of Fluorescence 6, no. 3 (1996): 147–57. http://dx.doi.org/10.1007/bf00732054.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Folaron, Margaret, Rendall Strawbridge, Kimberley S. Samkoe, Caroline Filan, David W. Roberts, and Scott C. Davis. "Elucidating the kinetics of sodium fluorescein for fluorescence-guided surgery of glioma." Journal of Neurosurgery 131, no. 3 (2019): 724–34. http://dx.doi.org/10.3171/2018.4.jns172644.

Full text
Abstract:
OBJECTIVEThe use of the optical contrast agent sodium fluorescein (NaFl) to guide resection of gliomas has been under investigation for decades. Although this imaging strategy assumes the agent remains confined to the vasculature except in regions of blood-brain barrier (BBB) disruption, clinical studies have reported significant NaFl signal in normal brain tissue, limiting tumor-to-normal contrast. A possible explanation arises from earlier studies, which reported that NaFl exists in both pure and protein-bound forms in the blood, the former being small enough to cross the BBB. This study aim
APA, Harvard, Vancouver, ISO, and other styles
9

Gauthier-Manuel, Bernard, Chafia Benmouhoub, and Bruno Wacogne. "Fluorescence Spectra of Prototropic Forms of Fluorescein and Some Derivatives and Their Potential Use for Calibration-Free pH Sensing." Sensors 24, no. 5 (2024): 1705. http://dx.doi.org/10.3390/s24051705.

Full text
Abstract:
Fluorescence pH sensing has proven to be efficient but with the drawback that molecules photobleach, requiring frequent calibrations. Double-emission peak molecules allow ratiometric measurements and theoretically avoid calibration. However, they are often expensive and fragile and usually have very low quantum yields. Single emission peaks such as fluorescein and derivatives are inexpensive and have very high quantum yields. Because they are single emission peaks, the pH is assumed to be derived from the ratio of emitted intensities at measured pH and at high pH values, i.e., they require fre
APA, Harvard, Vancouver, ISO, and other styles
10

McKenna, N., J. B. Meigs, and Y. L. Wang. "Identical distribution of fluorescently labeled brain and muscle actins in living cardiac fibroblasts and myocytes." Journal of Cell Biology 100, no. 1 (1985): 292–96. http://dx.doi.org/10.1083/jcb.100.1.292.

Full text
Abstract:
We have investigated whether living muscle and nonmuscle cells can discriminate between microinjected muscle and nonmuscle actins. Muscle actin purified from rabbit back and leg muscles and labeled with fluorescein isothiocyanate, and nonmuscle actin purified from lamb brain and labeled with lissamine rhodamine B sulfonyl chloride, were co-injected into chick embryonic cardiac myocytes and fibroblasts. When fluorescence images of the two actins were compared using filter sets selective for either fluorescein isothiocyanate or lissamine rhodamine B sulfonyl chloride, essentially identical patte
APA, Harvard, Vancouver, ISO, and other styles
11

Auel, Tobias, Linus Großmann, Lukas Schulig, Werner Weitschies, and Anne Seidlitz. "The EyeFlowCell: Development of a 3D-Printed Dissolution Test Setup for Intravitreal Dosage Forms." Pharmaceutics 13, no. 9 (2021): 1394. http://dx.doi.org/10.3390/pharmaceutics13091394.

Full text
Abstract:
An in vitro dissolution model, the so-called EyeFlowCell (EFC), was developed to test intravitreal dosage forms, simulating parameters such as the gel-like consistency of the vitreous body. The developed model consists of a stereolithography 3D-printed flow-through cell with a polyacrylamide (PAA) gel as its core. This gel needed to be coated with an agarose sheath because of its low viscosity. Drug release from hydroxypropyl methylcellulose-based implants containing either triamcinolone acetonide or fluorescein sodium was studied in the EFC using a schematic eye movement by the EyeMovementSys
APA, Harvard, Vancouver, ISO, and other styles
12

Kubrak, M. A., S. M. Zavhorodnii, M. B. Danyliuk, and A. I. Rylov. "Use of intraoperative luminescent methods for detecting affected lymph nodes in patients with complicated forms of colon cancer." Modern medical technology 16, no. 4 (2024): 267–72. https://doi.org/10.14739/mmt.2024.4.307038.

Full text
Abstract:
The issue of the volume of lymphatic dissection in patients with complicated forms of colon cancer and the possibility of using methods of intraoperative visualisation of the affected lymph nodes remains controversial. The aim of the study – to analyse the results of using the intraoperative luminescent method of detecting affected lymph nodes in patients with complicated forms of malignant diseases of the large intestine. Materials and methods. The study group included 109 patients with complicated forms of colon cancer. In the gender structure, female patients predominated – 57 (52.29 %), th
APA, Harvard, Vancouver, ISO, and other styles
13

Latif, Shoomaila, Rabia Rehman, Muhammad Imran, Shahid Iqbal, Ayesha Kanwal, and Liviu Mitu. "Removal of Acidic Dyes from Aqueous Media Using Citrullus Lanatus Peels: An Agrowaste-Based Adsorbent for Environmental Safety." Journal of Chemistry 2019 (March 13, 2019): 1–9. http://dx.doi.org/10.1155/2019/6704953.

Full text
Abstract:
In this work, removal of fluorescein and eosin dyes using common agrowaste, i.e., peels of water melon (Citrullus lanatus) (WMP), has been studied in the batch mode. The sorbent material (WMP) was characterized by using scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and elemental analysis. The sorbent was chemically modified by subjecting it to 0.1 N HNO3 and 0.1 N NaOH solutions. Different parameters such as sorbent dose, pH, temperature, and agitation speed were optimized to investigate the sorbent efficiency for fluorescein and eosin dyes.
APA, Harvard, Vancouver, ISO, and other styles
14

Zdończyk, Maria, Bartłomiej Potaniec, Marcin Skoreński, and Joanna Cybińska. "Development of Efficient One-Pot Methods for the Synthesis of Luminescent Dyes and Sol–Gel Hybrid Materials." Materials 15, no. 1 (2021): 203. http://dx.doi.org/10.3390/ma15010203.

Full text
Abstract:
This paper presents a comparison of the simultaneous preparation of di-O-alkylated and ether–ester derivatives of fluorescein using different methods (conventional or microwave heating). Shortening of the reaction time and increased efficiency were observed when using a microwave reactor. Moreover, described here for the first time is the application of a fast, simple, and eco-friendly ball-assisted method to exclusively obtain ether–ester derivatives. We also demonstrate that fluorescein can be effectively functionalized by O-alkylation carried out under microwave or ball-milling conditions,
APA, Harvard, Vancouver, ISO, and other styles
15

Tamulis, A., J. Tamuliene, M. L. Balevicius, Z. Rinkevicius, and V. Tamulis. "Quantum Mechanical Studies of Intensity in Electronic Spectra of Fluorescein Dianion and Monoanion Forms." Structural Chemistry 14, no. 6 (2003): 643–48. http://dx.doi.org/10.1023/b:stuc.0000007575.53499.d0.

Full text
APA, Harvard, Vancouver, ISO, and other styles
16

Pershin, B. S. "Differential diagnosis of active and inactive forms of cytomegalovirus retinitis." Modern technologies in ophtalmology, no. 3 (July 15, 2021): 384–87. http://dx.doi.org/10.25276/2312-4911-2021-3-384-387.

Full text
Abstract:
Purpose. Present the results of a study on the differential diagnosis of active and inactive forms of cytomegalovirus retinitis. Material and methods. 29 patients with cytomegalovirus retinitis, who underwent weekly fundus fluorescence angiography and intraocular fluid polymerase chain reaction during treatment. Results. Residual contrast fluorescence in the zone of cytomegalovirus retinitis foci was assessed on a scale from 0 to 4. The correlation between the intensity of contrast fluorescence in the fundus and the number of virus copies in the moisture of the anterior chamber was determined.
APA, Harvard, Vancouver, ISO, and other styles
17

Fernandes, Gregory E., Ya-Wen Chang, Akash Sharma, and Sarah Tutt. "One-Step Assembly of Fluorescence-Based Cyanide Sensors from Inexpensive, Off-The-Shelf Materials." Sensors 20, no. 16 (2020): 4488. http://dx.doi.org/10.3390/s20164488.

Full text
Abstract:
We report a simple and versatile approach to assemble sensitive and selective fluorescence “turn-on” sensors for cyanide by combining three off-the-shelf materials; namely fluorescent dye, 1-vinyl imidazole polymer, and cupric chloride. The cyanide-sensing species is a non-fluorescent fluorophore-polymer-Cu2+ complex; which forms as a result of the imidazole polymer’s ability to bind both fluorophore and fluorescence quencher (Cu2+). Cyanide removes Cu2+ from these complexes; thereby “turning-on” sensor fluorescence. These sensors are water-soluble and have a detection limit of ~2.5 μM (CN−) i
APA, Harvard, Vancouver, ISO, and other styles
18

Gerasimova, Marina A., Felix N. Tomilin, Elena Yu Malyar, et al. "Fluorescence and photoinduced proton transfer in the protolytic forms of fluorescein: Experimental and computational study." Dyes and Pigments 173 (February 2020): 107851. http://dx.doi.org/10.1016/j.dyepig.2019.107851.

Full text
APA, Harvard, Vancouver, ISO, and other styles
19

Probst, Mareike, Martin Schmidt, Werner Weitschies, and Anne Seidlitz. "In vitro simulation of distribution processes following intramuscular injection." Current Directions in Biomedical Engineering 2, no. 1 (2016): 383–86. http://dx.doi.org/10.1515/cdbme-2016-0085.

Full text
Abstract:
AbstractThere is an urgent need for in vitro dissolution test setups for intramuscularly applied dosage forms. Especially biorelevant methods are needed to predict the in vivo behavior of newly developed dosage forms in a realistic way. There is a lack of knowledge regarding critical in vivo parameters influencing the release and absorption behavior of an intramuscularly applied drug. In the presented work the focus was set on the simulation of blood perfusion and muscle tissue. A solid agarose gel, being incorporated in an open-pored foam, was used to mimic the gel phase of muscle tissue and
APA, Harvard, Vancouver, ISO, and other styles
20

Злобина, Anna Zlobina, Букина, et al. "GRADED CRITERIA OF DIAGNOSTICS AND PREDICTION OF CENTRAL SEROUS CHORIORETINOPATHY OUTCOME." Бюллетень Восточно-Сибирского научного центра Сибирского отделения Российской академии медицинских наук 1, no. 6 (2016): 131–36. http://dx.doi.org/10.12737/23794.

Full text
Abstract:
Present concept of central serous chorioretinopathy classification does not reflect the true picture of the pathological process. The purpose of the study was the development of diagnostic criteria of central serous chorioretinopathy clinical forms and basing on that extend and optimize its classification with assessment of risk factors and prognosis of disease. Full ophthalmologic and somatic examination of 56men with central serous chorioretinopathy was made. Focal laser coagulation was performed in patients with fluorescein leakage and local injury of pigment epithelium by angiographic data
APA, Harvard, Vancouver, ISO, and other styles
21

Angelides, K. J., K. E. Smith, and M. Takeda. "Assembly and exchange of intermediate filament proteins of neurons: neurofilaments are dynamic structures." Journal of Cell Biology 108, no. 4 (1989): 1495–506. http://dx.doi.org/10.1083/jcb.108.4.1495.

Full text
Abstract:
We have explored the dynamics of intermediate filament assembly and subunit exchange using fluorescently labeled neurofilament proteins and a fluorescence resonance energy transfer assay. Neurofilaments (NFs) are assembled from three highly phosphorylated proteins with molecular masses of 180 (NF-H), 130 (NF-M), and 66 kD (NF-L) of which NF-L forms the structural core. The core component, NF-L, was stoichiometrically labeled at cysteine 321 with fluorescein, coumarin, or biotin-maleimide to produce assembly-competent fluorescent or biotinylated derivatives, respectively. Using coumarin-labeled
APA, Harvard, Vancouver, ISO, and other styles
22

Aslamkhan, Amy G., Deborah M. Thompson, Jennifer L. Perry, et al. "The flounder organic anion transporter fOat has sequence, function, and substrate specificity similarity to both mammalian Oat1 and Oat3." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 291, no. 6 (2006): R1773—R1780. http://dx.doi.org/10.1152/ajpregu.00326.2006.

Full text
Abstract:
The flounder renal organic anion transporter (fOat) has substantial sequence homology to mammalian basolateral organic anion transporter orthologs (OAT1/Oat1 and OAT3/Oat3), suggesting that fOat may have functional properties of both mammalian forms. We therefore compared uptake of various substrates by rat Oat1 and Oat3 and human OAT1 and OAT3 with the fOat clone expressed in Xenopus oocytes. These data confirm that estrone sulfate is an excellent substrate for mammalian OAT3/Oat3 transporters but not for OAT1/Oat1 transporters. In contrast, 2,4-dichlorophenoxyacetic acid and adefovir are bet
APA, Harvard, Vancouver, ISO, and other styles
23

Hollingsworth, T. J., and Alecia K. Gross. "Innate and Autoimmunity in the Pathogenesis of Inherited Retinal Dystrophy." Cells 9, no. 3 (2020): 630. http://dx.doi.org/10.3390/cells9030630.

Full text
Abstract:
Inherited retinal dystrophies (RDs) are heterogenous in many aspects including genes involved, age of onset, rate of progression, and treatments. While RDs are caused by a plethora of different mutations, all result in the same outcome of blindness. While treatments, both gene therapy-based and drug-based, have been developed to slow or halt disease progression and prevent further blindness, only a small handful of the forms of RDs have treatments available, which are primarily for recessively inherited forms. Using immunohistochemical methods coupled with electroretinography, optical coherenc
APA, Harvard, Vancouver, ISO, and other styles
24

Spiegel, M., B. Vesti, A. Shore, U. K. Franzeck, F. Becker, and A. Bollinger. "Pressure of lymphatic capillaries in human skin." American Journal of Physiology-Heart and Circulatory Physiology 262, no. 4 (1992): H1208—H1210. http://dx.doi.org/10.1152/ajpheart.1992.262.4.h1208.

Full text
Abstract:
The network of lymphatic capillaries of the human skin was depicted at the distal part of the tibial plateau by fluorescence microlymphography (fluorescein isothiocyanate-dextran 150,000). Intralymphatic pressure was determined in 28 lymphatic capillaries of 21 healthy volunteers (mean diameter 56.0 +/- 10.0 microns) by a servo-nulling pressure system. It averaged 4.0 +/- 4.5 mmHg (range: -6.8 to +10.7 mmHg). These are the first measurements of pressure in the initial lymphatics of human skin and form a basis with which to compare measurements made in patients with different forms of edema.
APA, Harvard, Vancouver, ISO, and other styles
25

Zlatanović, Gordana, Dragan Veselinović, Sonja Cekić, Maja Živković, Jasmina Đorđević- Jocić, and Marko Zlatanović. "Ocular manifestation of rheumatoid arthritis-different forms and frequency." Bosnian Journal of Basic Medical Sciences 10, no. 4 (2010): 323–27. http://dx.doi.org/10.17305/bjbms.2010.2680.

Full text
Abstract:
Rheumatoid arthritis (RA) is a systemic inflammatory disease associated with a number of extra-articular organ manifestations. Ocular manifestations involved with RA are keratoconjunctivitis sicca, episcleritis, scleritis, corneal changes, and retinal vasculitus. The etiopathogenesis of this autoimmune disorder is still unknown. Aim of our study was to present different ocular manifestations of RA and their frequency. We have examined 691 patient with the diagnoses of RA. All examined patients were in I or II stage of the disease according to criteria of The American College of Rheumatology. O
APA, Harvard, Vancouver, ISO, and other styles
26

Trifanenkova, I. G., and A. V. Tereshhenko. "Iris fluorescein angiography in the diagnostics of active retinopathy of prematurity." Fyodorov journal of ophthalmic surgery, no. 4 (December 29, 2020): 67–72. http://dx.doi.org/10.25276/0235-4160-2020-4-67-72.

Full text
Abstract:
Purpose. To study the feature of the condition of the vessels of the iris according to fluorescence iris fluorescein angiography in various forms, stages and types of the course of active retinopathy of prematurity. Material and methods. Fluorescence iris fluorescein angiography was performed in 25 eyes (15 children). Children were born at 25–31 weeks of gestation. Children's weights were 680–1550 g. Children were 7–11 weeks old. 4 children (7 eyes) had a favorable type of course of 1–2 stages. 7 children (12 eyes) had an unfavorable type of course of 2–3 stages. 4 children (6 eyes) had aggres
APA, Harvard, Vancouver, ISO, and other styles
27

Musin, Egor V., Aleksandr L. Kim, Alexey V. Dubrovskii, Ekaterina B. Kudryashova, and Sergey A. Tikhonenko. "Decapsulation of Dextran by Destruction of Polyelectrolyte Microcapsule Nanoscale Shell by Bacillus subtilis Bacteria." Nanomaterials 10, no. 1 (2019): 12. http://dx.doi.org/10.3390/nano10010012.

Full text
Abstract:
One of the prerequisites of successful address delivery is controlling the release of encapsulated drugs. The new method of bacterial spore encapsulation in polyelectrolyte microcapsules allows for degrading the nanoscale membrane shell of microcapsules. The possibility of encapsulating spore forms of Bacillus subtilis in polystyrenesulfonate sodium/ polyallylamine hydrochloride (PSS/PAH) polyelectrolyte microcapsules was demonstrated. The activation and growth on a nutrient medium of encapsulated bacterial spores led to 60% degradation of the microcapsules nanoscale membrane shell. As a resul
APA, Harvard, Vancouver, ISO, and other styles
28

Stavrev, Vladimir N., Nelly P. Sivkova, and Desislava N. Koleva-Georgieva. "Optical Coherence Tomography-Angiography of Different Choroidal Neovascularization Subtypes in Wet Age-related Macular Degeneration." Folia Medica 61, no. 2 (2019): 317–26. http://dx.doi.org/10.2478/folmed-2018-0061.

Full text
Abstract:
Abstract Age-related macular degeneration is a leading cause of irreversible vision loss in individuals over 55 years of age worldwide. Conventionally, it is divided into two subtypes – dry (non-neovascular) and wet (neovascular) form. Neovascular age-related macular degeneration comprises only 10-15% of all patients but is responsible for more than 80% of blindness related to the disease. It requires early diagnosis and timely treatment. Fluorescein angiography is the current ‘gold standard’ for diagnosing neovascular forms. However, as an invasive procedure, it may be contraindicated in some
APA, Harvard, Vancouver, ISO, and other styles
29

Proudfoot, L., J. R. Kusel, H. V. Smith, W. Harnett, M. J. Worms, and M. W. Kennedy. "Rapid changes in the surface of parasitic nematodes during transition from pre- to post-parasitic forms." Parasitology 107, no. 1 (1993): 107–17. http://dx.doi.org/10.1017/s0031182000079464.

Full text
Abstract:
SummaryAll mammalian-parasitic stages of a range of nematode species investigated (Brugia pahangi, Acanthocheilonema viteae, Strongyloides ratti, Nippostrongylus brasiliensis, Trichinella spiralisandOstertagia ostertagi) labelled in a surface-restricted manner with the fluorescent lipid analogues 5-N-(octadecanoyl)aminofluorescein (AF18) or nitrobenzoxadiazole-cholesterol (NBD-chol), but failed to bind other similar probes. In contrast, the surfaces of the ‘pre-parasitic’ infective stages of these species had affinity for neither AF18 nor NBD-chol. This exclusion of lipid analogues changed rap
APA, Harvard, Vancouver, ISO, and other styles
30

Amin, A., H. Khalli, and H. Saleh. "Spectrophotometric Estimation of Cefuroxime and Ceftazidime in Bulk and in Dosage Forms." Scientia Pharmaceutica 69, no. 2 (2001): 143–50. http://dx.doi.org/10.3797/scipharm.aut-01-15.

Full text
Abstract:
Three simple, accurate and sensitive spectrophotometric methods (A, B and C) for the determination of cefuroxime and ceftazidime in bulk samples and in dosage forms are described. They are based on the reaction with nitrous acid forming a nitroso derivatives which can be measured at λmax 350 and 355 nm for cefuroxime (I) and ceftazidime (II), respectively (method A) or by oxidation of drug I or II with an excess of freshly prepared hypobromite and the residual hypobromite was treated with sodium fluorescein at the optimum experimental conditions and measured at λmax at 517 nm (method B). Metho
APA, Harvard, Vancouver, ISO, and other styles
31

Natsheh, Hiba, and Elka Touitou. "Improved Efficiency of Pomegranate Seed Oil Administrated Nasally." Pharmaceutics 14, no. 5 (2022): 918. http://dx.doi.org/10.3390/pharmaceutics14050918.

Full text
Abstract:
Pomegranate seed oil (PSO) is currently administrated orally as a food supplement for improving memory. However, the efficiency of the oral dosage forms for such purposes is low, mainly due to the blood brain barrier impeding a good delivery to brain. In this work, we designed and characterized a PSO phospholipid oily gel for nasal administration. We tested the performance of the new PSO delivery system in animal models for impaired memory and locomotor activity. The experimental results indicated a statistically significant improvement (p < 0.05) of more than 1.5 fold in the behavior of an
APA, Harvard, Vancouver, ISO, and other styles
32

Ku, Tien-Hsiung, Wen-Ting Shen, Chien-Te Hsieh, Grace Shiahuy Chen, and Wei-Chung Shia. "Specific Forms of Graphene Quantum Dots Induce Apoptosis and Cell Cycle Arrest in Breast Cancer Cells." International Journal of Molecular Sciences 24, no. 4 (2023): 4046. http://dx.doi.org/10.3390/ijms24044046.

Full text
Abstract:
Graphene quantum dots (GQDs), nanomaterials derived from graphene and carbon dots, are highly stable, soluble, and have exceptional optical properties. Further, they have low toxicity and are excellent vehicles for carrying drugs or fluorescein dyes. Specific forms of GQDs can induce apoptosis and could be used to treat cancers. In this study, three forms of GQDs (GQD (nitrogen:carbon = 1:3), ortho-GQD, and meta-GQD) were screened and tested for their potential to inhibit breast cancer cell (MCF-7, BT-474, MDA-MB-231, and T-47D) growth. All three GQDs decreased cell viability after 72 h of tre
APA, Harvard, Vancouver, ISO, and other styles
33

Moskaeva, Elena, Kirill Ostrovskiy, Sergey Shekhovtsov, and Nikolay Mchedlov-Petrossyan. "Transmittance of electronic effects in the fluorescein molecule: nitro and amino groups in the phthalic acid residue." Kharkov University Bulletin Chemical Series, no. 36 (February 17, 2021): 24–32. http://dx.doi.org/10.26565/2220-637x-2021-36-05.

Full text
Abstract:
This paper is aimed to estimate the transmittance of the electronic effects in the fluorescein molecule between the phthalic acid residue and the xanthene moiety. Despite the almost orthogonal orientation of the latter with respect to the rest of the molecule, some influence of substituents in this 9-aryl ring on the dissociation of the hydroxyl group of the hydroxyxanthene cannot be ruled out. In order to reveal this (possible) effect, we blocked the carboxylic group via esterification. The reason of using dimethyl sulfoxide as solvent was the high Hammett’s “rho” constant for phenolic group.
APA, Harvard, Vancouver, ISO, and other styles
34

Alhibah, Mohammad, Marius Kröger, Sabine Schanzer, et al. "Penetration Depth of Propylene Glycol, Sodium Fluorescein and Nile Red into the Skin Using Non-Invasive Two-Photon Excited FLIM." Pharmaceutics 14, no. 9 (2022): 1790. http://dx.doi.org/10.3390/pharmaceutics14091790.

Full text
Abstract:
The stratum corneum (SC) forms a strong barrier against topical drug delivery. Therefore, understanding the penetration depth and pathways into the SC is important for the efficiency of drug delivery and cosmetic safety. In this study, TPT-FLIM (two-photon tomography combined with fluorescence lifetime imaging) was applied as a non-invasive optical method for the visualization of skin structure and components to study penetration depths of exemplary substances, like hydrophilic propylene glycol (PG), sodium fluorescein (NaFl) and lipophilic Nile red (NR) into porcine ear skin ex vivo. Non-fluo
APA, Harvard, Vancouver, ISO, and other styles
35

Rosenbaum, Christoph, Naemi Gerds, Liliane Hack, and Werner Weitschies. "Scalability of API-Loaded Multifilament Yarn Production by Hot-Melt Extrusion and Evaluation of Fiber-Based Dosage Forms." Pharmaceutics 16, no. 8 (2024): 1103. http://dx.doi.org/10.3390/pharmaceutics16081103.

Full text
Abstract:
Fiber-based technologies are widely used in various industries, but their use in pharmaceuticals remains limited. While melt extrusion is a standard method for producing medical fibers such as sutures, it is rarely used for pharmaceutical fiber-based dosage forms. The EsoCap system is a notable exception, using a melt-extruded water-soluble filament as the drug release trigger mechanism. The challenge of producing drug-loaded fibers, particularly due to the use of spinning oils, and the processing of the fibers are addressed in this work using other approaches. The aim of this study was to dev
APA, Harvard, Vancouver, ISO, and other styles
36

Messmann, R., R. Amato, J. Hernandez-McClain та ін. "A phase II study of FolateImmune (EC90 with GP1–0100 adjuvant followed by EC17) with low dose cytokines interleukin-2 (IL-2) and interferon-α (IFN-α) in patients with refractory or metastatic cancer". Journal of Clinical Oncology 25, № 18_suppl (2007): 13516. http://dx.doi.org/10.1200/jco.2007.25.18_suppl.13516.

Full text
Abstract:
13516 Background: FolateImmune is a folate-receptor (FR)-targeted immunotherapy that induces an immune response against tumor cells by marking them with a folate-hapten conjugate. The conjugate is specifically designed to target FR, which is over-expressed in a variety of cancers. FolateImmune therapy is comprised of a vaccine fluorescein conjugate (EC90), an adjuvant (GP-0100), and a folate-hapten conjugate (EC17). Patients (pts) receive a series of subcutaneous (SQ) injections of EC90 vaccine to stimulate the production of antibodies to the fluorescein-hapten, followed by SQ injections of EC
APA, Harvard, Vancouver, ISO, and other styles
37

Aryal, Manish, Preetty Pranatharthiharan, and Peter M. Muriana. "Optimization of a Microplate Assay for Generating Listeria Monocytogenes, E. Coli O157:H7, and Salmonella Biofilms and Enzymatic Recovery for Enumeration." Foods 8, no. 11 (2019): 541. http://dx.doi.org/10.3390/foods8110541.

Full text
Abstract:
Biofilms enable the persistence of pathogens in food processing environments. Sanitizing agents are needed that are effective against pathogens entrapped in biofilms that are more difficult to inactivate than planktonic cells that are displaced and found on equipment surfaces. We examined conditions to develop, analyze, and enumerate the enhanced biofilms of three different foodborne pathogens assisted by fluorescence adherence assay and enzymatic detachment. We compared three different isomeric forms of fluorescent substrates that are readily taken up by bacterial cells based on carboxy-fluor
APA, Harvard, Vancouver, ISO, and other styles
38

Wang, Tegexibaiyin, Wen Zhang, Xiaofeng Zhang, et al. "Synthesis of Fluorescent Carbon Dots by Gastrointestinal Fluid Treatment of Mongolia Har Gabur." Journal of Nanomaterials 2017 (2017): 1–7. http://dx.doi.org/10.1155/2017/8575162.

Full text
Abstract:
Har Gabur is the carbide obtained from pig manure by burning. The fluorescent carbon dots (CDs) of Har Gabur were successfully synthesized through simulating the digestion process of human gastrointestinal tract. Transmission Electron Microscope (TEM) analysis showed that the average size of the prepared Har Gabur CDs was 4 nm, with good solubility in water and strong fluorescence under UV irradiation. The X-ray and Raman results showed that the Har Gabur CDs were mainly composed of oxygen “O” and carbon “C” elements, in the forms of “C=O” and “C-O.” The bond energy results showed that the nit
APA, Harvard, Vancouver, ISO, and other styles
39

Suner, Selin S., Venkat R. Bhethanabotla, Ramesh S. Ayyala, and Nurettin Sahiner. "Rapid Pathogen Purge by Photosensitive Arginine–Riboflavin Carbon Dots without Toxicity." Materials 16, no. 19 (2023): 6512. http://dx.doi.org/10.3390/ma16196512.

Full text
Abstract:
Photo-activatable antipathogenic carbon dots (CDs) were prepared by carbonization of citric acid and arginine (Arg) via 3 min microwave treatment for use in the eradication of common microorganisms. Nitrogen-doped Arg CDs were spherical in shape with a size range of 0.5 to 5 nm. The Arg CDs were modified with fluorescent dyes, such as fluorescein sodium salt (FSS, as Arg-FSS) and riboflavin (RBF, as Arg-RBF), to improve antimicrobial potency by enhancing their application in photodynamic therapy. The modified Arg CDs afforded fluorescence emission properties at 520 nm in the green region in ad
APA, Harvard, Vancouver, ISO, and other styles
40

Sotnikov, Dmitriy V., Nadezhda A. Byzova, Anatoly V. Zherdev, and Boris B. Dzantiev. "Ability of Antibodies Immobilized on Gold Nanoparticles to Bind Small Antigen Fluorescein." International Journal of Molecular Sciences 24, no. 23 (2023): 16967. http://dx.doi.org/10.3390/ijms242316967.

Full text
Abstract:
The analytical applications of antibodies are often associated with their immobilization on different carriers, which is accompanied by a loss of antigen-binding activity for a sufficient proportion of the bound antibodies. In contrast to data on plain carriers, minimal data are available on the properties of antibodies on the surfaces of nanoparticles. Protein antigens have been predominantly investigated, for which space restrictions do not allow them to occupy all active sites of immobilized antibodies. This study considered a low-molecular-weight compound, fluorescein, as an antigen. Spher
APA, Harvard, Vancouver, ISO, and other styles
41

Troisi, Mario, Ciro Costagliola, Michele Rinaldi, Diego Strianese, Elisabetta Chiariello Vecchio, and Salvatore Troisi. "Efficacy and Safety of Keratosept Eye Drops in Patients with Punctate Keratitis: Clinical and Microbiological Evaluation on 50 Eyes." Microorganisms 12, no. 11 (2024): 2277. http://dx.doi.org/10.3390/microorganisms12112277.

Full text
Abstract:
We evaluated the efficacy and safety of eye drops with antiseptic and re-epithelizing properties (Keratosept®, Bruschettini, Genova, Italy) on 50 eyes affected by punctate keratitis of suspected microbial origin. A biomicroscopic examination, fluorescein dye staining test (Fluotest), tear break-up time test (TBUT), and the ocular surface disease Index (OSDI) questionnaire were used to assess treatment efficacy. Treatment success was defined as a negative Fluotest and an OSDI score <12 on the 15th day of treatment. According to this definition, Keratosept®, either alone or in combination wit
APA, Harvard, Vancouver, ISO, and other styles
42

Kunapuli, Satya, Daniel Walz, Robert Colman, and Raul DeLa Cadena. "Expression of Thrombospondin 1 on the Surface of Activated Platelets Mediates their Interaction with the Heavy Chains of Human Kininogens through Lys 244-Pro 254." Thrombosis and Haemostasis 79, no. 01 (1998): 186–94. http://dx.doi.org/10.1055/s-0037-1614248.

Full text
Abstract:
SummaryPlatelet thrombospondin (TSP1) forms a complex with high (HK) and low (LK) molecular weight kininogens. We isolated a proteolytic fragment from HK and LK heavy chains (12 kDa) recognized by TSP1 with a N-terminal sequence, K244ICVGCPRDIP254. Lys244-Pro254 oxidized to cyclic form prevented binding of 125I-LK to TSP1. This effect was abolished by reduction and alkylation. Oxidized peptide KICVGCPRDIP (100 μM) reversed the known inhibitory effects of LK or HK (1 μM), on thrombin-induced platelet activation, suggesting this peptide forms part of the cell binding site on HK and LK for activa
APA, Harvard, Vancouver, ISO, and other styles
43

Hozak, P., A. M. Sasseville, Y. Raymond, and P. R. Cook. "Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cells." Journal of Cell Science 108, no. 2 (1995): 635–44. http://dx.doi.org/10.1242/jcs.108.2.635.

Full text
Abstract:
The nuclear lamina forms a protein mesh that underlies the nuclear membrane. In most mammalian cells it contains the intermediate filament proteins, lamins A, B and C. As their name indicates, lamins are generally thought to be confined to the nuclear periphery. We now show that they also form part of a diffuse skeleton that ramifies throughout the interior of the nucleus. Unlike their peripheral counterparts, these internal lamins are buried in dense chromatin and so are inaccessible to antibodies, but accessibility can be increased by removing chromatin. Knobs and nodes on an internal skelet
APA, Harvard, Vancouver, ISO, and other styles
44

Hollingsworth, T. J., Meredith G. Hubbard, Hailey J. Levi, et al. "Proinflammatory Pathways Are Activated in the Human Q344X Rhodopsin Knock-In Mouse Model of Retinitis Pigmentosa." Biomolecules 11, no. 8 (2021): 1163. http://dx.doi.org/10.3390/biom11081163.

Full text
Abstract:
Retinitis pigmentosa (RP) is a hereditary disease of the retina that results in complete blindness. Currently, there are very few treatments for the disease and those that exist work only for the recessively inherited forms. To better understand the pathogenesis of RP, multiple mouse models have been generated bearing mutations found in human patients including the human Q344X rhodopsin knock-in mouse. In recent years, the immune system was shown to play an increasingly important role in RP degeneration. By way of electroretinography, optical coherence tomography, funduscopy, fluorescein angio
APA, Harvard, Vancouver, ISO, and other styles
45

Lutsenko, N. S., O. A. Isakova, O. A. Rudycheva, T. S. Kyrilova, and T. S. Mihalchik. "SOME QUESTIONS ABOUT THE STATE OF THE EYE SURFACE IN PATIENTS BEFORE CATARACT SURGERY." Modern medical technologies 46, no. 3 (2020): 21. http://dx.doi.org/10.34287/mmt.3(46).2020.4.

Full text
Abstract:
Introduction. Ocular surface status is a main risk factor for the development of postoperative complications and lead to the decrease of life quality. Purpose of the study.The condition of the surface of the eye in patients before cataract surgery was study. Material and methods. 320 patients preoperative the cataract surgery were investigated, among them 172 patients were female, 148 – were male. Patients with any previous ocular surgery or concomitant ocular diseases were excluded. The structure of common non-ocular comorbidities, subjective signs of ocular surface damage (redness, foreign b
APA, Harvard, Vancouver, ISO, and other styles
46

Li, Yangjie, Site Luo, Yongqiang Gui, Xin Wang, Ziyuan Tian, and Haihu Yu. "Difunctional Hydrogel Optical Fiber Fluorescence Sensor for Continuous and Simultaneous Monitoring of Glucose and pH." Biosensors 13, no. 2 (2023): 287. http://dx.doi.org/10.3390/bios13020287.

Full text
Abstract:
It is significant for people with diabetes to know their body’s real-time glucose level, which can guide the diagnosis and treatment. Therefore, it is necessary to research continuous glucose monitoring (CGM) as it gives us real-time information about our health condition and its dynamic changes. Here, we report a novel hydrogel optical fiber fluorescence sensor segmentally functionalized with fluorescein derivative and CdTe QDs/3-APBA, which can continuously monitor pH and glucose simultaneously. In the glucose detection section, the complexation of PBA and glucose will expand the local hydro
APA, Harvard, Vancouver, ISO, and other styles
47

Peixoto, Pablo M., Shin-Young Ryu, Agnes Bombrun, Bruno Antonsson, and Kathleen W. Kinnally. "MAC inhibitors suppress mitochondrial apoptosis." Biochemical Journal 423, no. 3 (2009): 381–87. http://dx.doi.org/10.1042/bj20090664.

Full text
Abstract:
MAC (mitochondrial apoptosis-induced channel) forms in the mitochondrial outer membrane and unleashes cytochrome c to orchestrate the execution of the cell. MAC opening is the commitment step of intrinsic apoptosis. Hence closure of MAC may prevent apoptosis. Compounds that blocked the release of fluorescein from liposomes by recombinant Bax were tested for their ability to directly close MAC and suppress apoptosis in FL5.12 cells. Low doses of these compounds (IC50 values ranged from 19 to 966 nM) irreversibly closed MAC. These compounds also blocked cytochrome c release and halted the onset
APA, Harvard, Vancouver, ISO, and other styles
48

Jenkins, P. Vincent, Jan Freas, Kyla M. Schmidt, Qian Zhou, and Philip J. Fay. "Mutations associated with hemophilia A in the 558-565 loop of the factor VIIIa A2 subunit alter the catalytic activity of the factor Xase complex." Blood 100, no. 2 (2002): 501–8. http://dx.doi.org/10.1182/blood-2001-12-0361.

Full text
Abstract:
Abstract The 558-565 loop region in the A2 subunit of factor (F) VIIIa forms a direct interface with FIXa. We have expressed and purified B-domainless FVIII (FVIIIWT) and B-domainless FVIII containing the hemophilia A–associated mutations Ser558Phe, Val559Ala, Asp560Ala, Gln565Arg, and the activated protein C cleavage site mutant Arg562Ala. Titration of FVIIIa in FXa generation assays showed that the mutant and wild-type proteins had similar functional affinities for FIXa (dissociation constant [Kd] values ∼5 nM-20 nM and ∼100 nM-250 nM in the presence and absence of phospholipid, respectively
APA, Harvard, Vancouver, ISO, and other styles
49

Triantafilou, Martha, Kathy Triantafilou, and Nelson Fernandez. "Rough and smooth forms of fluorescein-labelled bacterial endotoxin exhibit CD14/LBP dependent and independent binding that is influencedby endotoxin concentration." European Journal of Biochemistry 267, no. 8 (2000): 2218–26. http://dx.doi.org/10.1046/j.1432-1327.2000.01222.x.

Full text
APA, Harvard, Vancouver, ISO, and other styles
50

Mukhametova, L. I., S. A. Eremin, I. V. Mikhura, et al. "Single-Domain Nanobodies for Determination of Conformational Changes in Transferrin and Their Use in Fluorescent Polarization Immunoassay." Russian Journal of Bioorganic Chemistry 51, no. 2 (2025): 702–11. https://doi.org/10.1134/s1068162024605470.

Full text
Abstract:
Abstract Objective: Transferrin (Tf) exists in two forms in blood plasma: iron-containing holo-Tf and iron-free apo-Tf forms. An important biochemical marker of diseases associated with iron deficiency or excess is the quantitative ratio of these forms in human blood plasma. Methods: Application of the fluorescence polarization immunoassay (FPIA) method and the use of recombinant camel nanobodies as a recognition reagent for the rapid determination of holo-Tf and apo-Tf will allow the development of a rapid method for determining two transferrin conformations. Results and Discussion: Conjugate
APA, Harvard, Vancouver, ISO, and other styles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!