Log in

Relevant bibliographies by topics / Fluorescein isothiocyanate

Contents

Journal articles
Dissertations / Theses
Book chapters
Conference papers

Academic literature on the topic 'Fluorescein isothiocyanate'

Author: Grafiati

Published: 24 April 2022

Last updated: 30 July 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Fluorescein isothiocyanate.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Fluorescein isothiocyanate"

1

Spiguel, Lisa, Christiana Shaw, Adam Katz, et al. "Fluorescein Isothiocyanate." Annals of Plastic Surgery 78 (June 2017): S296—S298. http://dx.doi.org/10.1097/sap.0000000000001034.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Kaewsaneha, Chariya, Kulachart Jangpatarapongsa, Tienrat Tangchaikeeree, Duangporn Polpanich, and Pramuan Tangboriboonrat. "Fluorescent chitosan functionalized magnetic polymeric nanoparticles: Cytotoxicity and in vitro evaluation of cellular uptake." Journal of Biomaterials Applications 29, no. 5 (2014): 761–68. http://dx.doi.org/10.1177/0885328214540349.

Full text

Abstract:

Nanoparticles possessing magnetic and fluorescent properties were fabricated by the covalent attachment of fluorescein isothiocyanate onto magnetic polymeric nanoparticles functionalized by chitosan. The synthesized magnetic polymeric nanoparticles-chitosan/fluorescein isothiocyanate were successfully used for labeling the living organ and blood-related cancer cells, i.e., HeLa, Hep G2, and K562 cells. The cytotoxicity test of nanoparticles at various incubation times indicated the high cell viability (>90%) without morphological change. The confocal microscopy revealed that they could pass

APA, Harvard, Vancouver, ISO, and other styles

3

Chin, Suk Fun, Aressa Azman, Suh Cem Pang, and Sing Muk Ng. "Fluorescein-Labeled Starch Maleate Nanoparticles as Sensitive Fluorescent Sensing Probes for Metal Ions." Journal of Nanomaterials 2014 (2014): 1–8. http://dx.doi.org/10.1155/2014/108359.

Full text

Abstract:

Fluorescein 5(6)-isothiocyanate starch maleate (FISM) nanoparticles were prepared by covalently attached fluorescein 5(6)-isothiocyanate (FITC) with starch maleate. FISM nanoparticles with a mean particle size of 87 nm were formed via self-assembly upon precipitation in ethanolic solution. FISM nanoparticles were strongly fluorescent with maximum emission wavelength of 518 nm. The fluorescence of FISM nanoparticles can be quenched by silver (Ag+) and lead (Pb2+) ions in a concentration dependent manner. We have demonstrated the first use of FISM nanoparticles as cheap and effective fluorescent

APA, Harvard, Vancouver, ISO, and other styles

4

Wang, Jing, Meng Ma, Rongbin Huang, Ligeng Wang, Aimin Chen, and Jun Hu. "An efficient ratiometric fluorescent probe based on dual-emission fluorescent silica nanoparticles for visual determination of Hg2+." Analytical Methods 7, no. 6 (2015): 2295–99. http://dx.doi.org/10.1039/c4ay02905d.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Wiley, J. S., A. M. Brocklebank, M. B. Snook, et al. "A new fluorescent probe for the equilibrative inhibitor-sensitive nucleoside transporter. 5′-S-(2-aminoethyl)-N6-(4-nitrobenzyl)-5′-thioadenosine (SAENTA)-x2-fluorescein." Biochemical Journal 273, no. 3 (1991): 667–72. http://dx.doi.org/10.1042/bj2730667.

Full text

Abstract:

The N6-(4-nitrobenzyl) derivative of adenosine is a tight-binding inhibitor of the equilibrative inhibitor-sensitive nucleoside transporter of mammalian cells. A fluorescent ligand for this transporter has been synthesized by allowing an adenosine analogue. 5′-S-(2-aminoethyl)-N6-(4-nitrobenzyl)-5′-thioadenosine (SAENTA), to react with fluorescein isothiocyanate. The purified adduct had a SAENTA/fluorescein molar ratio of 0.92:1 calculated from its absorption spectrum. The intensity of fluorescent emission from the SAENTA-chi 2-fluorescein adduct was 30% that of fluorescein isothiocyanate (chi

APA, Harvard, Vancouver, ISO, and other styles

6

Malatesti, Nela, Robert Hudson, Karen Smith, et al. "Isothiocyanato Boron Dipyrromethenes—The First BODIPY Analogues of Fluorescein Isothiocyanate." Photochemistry and Photobiology 82, no. 3 (2006): 746. http://dx.doi.org/10.1562/2005-01-10-ra-769.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

ADAMO, Hugo P., Adelaida G. FILOTEO, and John T. PENNISTON. "The plasma membrane Ca2+ pump mutant lysine591 → arginine retains some activity, but is still inactivated by fluorescein isothiocyanate." Biochemical Journal 317, no. 1 (1996): 41–44. http://dx.doi.org/10.1042/bj3170041.

Full text

Abstract:

Inactivation of the wild-type human plasma membrane Ca2+ pump (isoform 4b) by fluorescein isothiocyanate is accompanied by covalent modification of Lys591. The mutation of Lys591 to arginine reduced the Ca2+ transport activity to 35% of the wild-type, and diminished the amount of acylphosphate formed from ATP by a corresponding amount. When this mutant was treated with fluorescein isothiocyanate, the enzyme was still irreversibly inactivated, even though no reactive residue was available at position 591. The results show that, although Ca2+ pump function is sensitive to the residue at position

APA, Harvard, Vancouver, ISO, and other styles

8

Pauls, K. P., and P. V. Chuong. "Flow cytometric identification of Brassica napus protoplast fusion products." Canadian Journal of Botany 65, no. 5 (1987): 834–38. http://dx.doi.org/10.1139/b87-113.

Full text

Abstract:

A flow cytometric method to identify Brassica somatic hybrids has been developed. The procedure is based on the use of fluorescein isothiocyanate stained hypocotyl protoplasts and unstained mesophyll protoplasts as partners for polyethylene glycol induced protoplast fusion. The fluorescein isothiocyanate stained hypocotyl protoplasts and unstained mesophyll protoplasts were passed through the flow cytometer – cell sorter separately to maximize the sensitivity of the red and green detectors to chlorophyll and fluorescein isothiocyanate fluorescence, respectively, and to ensure that there was no

APA, Harvard, Vancouver, ISO, and other styles

9

Fratoddi, Ilaria, Chiara Battocchio, Giovanna Iucci, et al. "Silver Nanoparticles Functionalized by Fluorescein Isothiocyanate or Rhodamine B Isothiocyanate: Fluorescent and Plasmonic Materials." Applied Sciences 11, no. 6 (2021): 2472. http://dx.doi.org/10.3390/app11062472.

Full text

Abstract:

This paper presents the synthesis of silver nanoparticles (AgNPs) functionalized with fluorescent molecules, in particular with xanthene-based dyes, i.e., fluorescein isothiocyanate (FITC, λmax = 485 nm) and rhodamine B isothiocyanate (RITC, λmax = 555 nm). An in-depth characterization of the particle–dye systems, i.e., AgNPs–RITC and AgNPs–FITC, is presented to evaluate their chemical structure and optical properties due to the interaction between their plasmonic and absorption properties. UV–Vis spectroscopy and the dynamic light scattering (DLS) measurements confirmed the nanosize of the Ag

APA, Harvard, Vancouver, ISO, and other styles

10

Gatto, C., and M. A. Milanick. "Inhibition of the red blood cell calcium pump by eosin and other fluorescein analogues." American Journal of Physiology-Cell Physiology 264, no. 6 (1993): C1577—C1586. http://dx.doi.org/10.1152/ajpcell.1993.264.6.c1577.

Full text

Abstract:

This paper addresses the mechanism of inhibition of the plasma membrane Ca pump by fluorescein analogues and their isothiocyanate derivatives. Eosin (i.e., tetrabromofluorescein) was found to be one of the most potent reversible inhibitors of the erythrocyte Ca pump [half-maximal inhibitory concentration (IC50) < 0.2 microM]; fluorescein itself was about four orders of magnitude less potent (IC50 approximately 1,000 microM). Eosin decreased the maximum influx and thus did not compete with ATP for the Ca pump. Irreversible inhibition produced by the isothiocyanate analogues of eosin and fluo

APA, Harvard, Vancouver, ISO, and other styles

More sources

Dissertations / Theses on the topic "Fluorescein isothiocyanate"

1

Chasser, Kaylin M. "Effect of day of hatch inoculation with Enterobacteriaceae on inflammation and enteric permeability in broilers." The Ohio State University, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1618943283197541.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Ireland, Ian D. "High sensitivity protein sequencing using fluorescein isothiocyanate for derivatization on a miniaturized sequencer using capillary electrophoresis with laser induced fluorescence detection for product identification." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0009/NQ59975.pdf.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Jacksén, Johan. "Improved techniques for CE and MALDI-MS including microfluidic hyphenations foranalysis of biomolecules." Doctoral thesis, KTH, Analytisk kemi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-27342.

Full text

Abstract:

In this thesis, improved techniques for biomolecule analysis using capillary electrophoresis (CE) and matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) and hyphenations between those have been presented.A pre-concentration method which is possible to apply in both techniques, has also been investigated. In this work the off-line MS mode has been used either in the form of fractionation (Paper I) or by incorporating the MALDI target in the CE separation system (Paper II).In Paper I, a protocol for CE-MALDI analysis of cyanogen bromide digested bacteriorhodopsin (BR) pepti

APA, Harvard, Vancouver, ISO, and other styles

4

Sinigaglia, Giulietta. "Sviluppo ed applicazioni biomediche di sistemi nanostrutturati superparamagnetici costituiti da maghemite (SAMN, Surface Active Maghemite Nanoparticles) e bioelementi." Doctoral thesis, Università degli studi di Padova, 2012. http://hdl.handle.net/11577/3425446.

Full text

Abstract:

With the rapid development of nanotechnology, magnetic nanoparticles are currently being widely studied. It has long been known that the physico-chemical properties of magnetic nanoparticles can be vastly different from those of the corresponding bulk material. In particular, nanoparticles characteristics are: large surface-to-volume ratio, high surface reaction activity, high catalytic efficiency, and strong adsorption ability, which are helpful for the immobilization of desired biosensing molecules. The nanoparticles used in the present research project (called surface active maghemite nanop

APA, Harvard, Vancouver, ISO, and other styles

5

Cai, sheng-yun, and 蔡昇運. "The Study on Fluorescein Isothiocyanate Dye Coated Nano Silica Particles." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/95870881244638295471.

Full text

Abstract:

碩士<br>逢甲大學<br>紡織工程所<br>96<br>The research prepared nano silica particles by Sol-gel method, and used APS(3-Aminopropyltriethoxysilane) to modify the surface of silica particles. The organic dyes of fluoresce was covalently attached to the surface of the inorganic silica and formed the fluoresce silica particles. In order to enhance the fluoresce strength performance, we prepared nano silica particles by different preparation conditions: the amounts of Tetraethyl Orthosilicate (TEOS), the concentration of ammonia et al.. The average particle diameter of nano silica particles was 120 nm from S

APA, Harvard, Vancouver, ISO, and other styles

6

Hu, Chieh-shen, and 胡接燊. "Development of chondroitin sulfate chitosan nanoparticles drug delivery system platform: fluorescein isothiocyanate conjugated bovine serum albumin (FITC-BSA) and doxorubicin as model drugs." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/d5p6fe.

Full text

Abstract:

博士<br>國立臺灣科技大學<br>應用科技研究所<br>103<br>In this study, chondroitin sulfate (CHS)–chitosan (CS) nanoparticles (NPs) were prepared as a drug delivery carrier. FITC-BSA and Doxorubicin (Dox) were used as model drugs to be loaded in the CHS-CS NPs for further investigation.CHS-CS NPs were fabricated by ionic crosslinking of CS solution with CHS. The physicochemical properties and biological activities of the prepared CHS-CS NPs, such as the drug release profile, cell cytotoxicity, cellular internalization, and in vivo anti-tumor effects were evaluated. The CHS–CS NPs had mean sizes of 250±17, 238±6,

APA, Harvard, Vancouver, ISO, and other styles

7

Gan, Shao MIng. "Marquage fluorescent des protéines pour étudier les enzymes protéolytiques solubles et immobilisées par la cartographie peptidique électrophorétique." Thèse, 2010. http://hdl.handle.net/1866/4510.

Full text

Abstract:

La cartographie peptidique est une méthode qui permet entre autre d’identifier les modifications post-traductionnelles des protéines. Elle comprend trois étapes : 1) la protéolyse enzymatique, 2) la séparation par électrophorèse capillaire (CE) ou chromatographie en phase liquide à haute performance (HPLC) des fragments peptidiques et 3) l’identification de ces derniers. Cette dernière étape peut se faire par des méthodes photométriques ou par spectrométrie de masse (MS). Au cours de la dernière décennie, les enzymes protéolytiques immobilisées ont acquis une grande popularité parce qu’elles p

APA, Harvard, Vancouver, ISO, and other styles

Book chapters on the topic "Fluorescein isothiocyanate"

1

Muramoto, Koji, Kiyoshi Nokihara, Akira Ueda, and Hisao Kamiya. "Sensitization of Gas-Phase Protein Sequencer using Fluorescein Isothiocyanate (FITC)." In Methods in Protein Sequence Analysis. Springer US, 1993. http://dx.doi.org/10.1007/978-1-4899-1603-7_4.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Faller, Larry D., Irina N. Smirnova, Shwu-Hwa Lin, and Martin Stengelin. "Mechanism of the Conformational Change in Fluorescein 5′-Isothiocyanate-Modified Na+/K+ ATPase." In The Sodium Pump. Steinkopff, 1994. http://dx.doi.org/10.1007/978-3-642-72511-1_106.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Loken, Michael R., Cherie L. Green, and Denise A. Wells. "lmmunofluorescence of surface markers." In Flow Cytometry. Oxford University PressOxford, 2000. http://dx.doi.org/10.1093/oso/9780199638253.003.0005.

Full text

Abstract:

Abstract The discovery of monoclonal antibodies by Kohler and Milstein in 1975 dramatically enhanced the use of immunofluorescence for identifying cell-surface antigens. When applied in the flow cytometric analysis of heterogeneous populations, these highly specific monoclonal antibodies have broadened our understanding of diseases such as the progression of HIV infection, the origin and nature of leukaemia and lymphoma, and the regulation of haematopoietic cell differentiation and maturation. The discovery of new fluorescent chromophores has further exploited the power of quantitative cellula

APA, Harvard, Vancouver, ISO, and other styles

4

Kaewphinit, Thongchai, Somchai Santiwatanakul, and Kosum Chansiri. "The Detection of Tuberculosis by Loop-Mediated Isothermal Amplification (LAMP) Combined with a Lateral Flow Dipstick." In Handbook of Research on Diverse Applications of Nanotechnology in Biomedicine, Chemistry, and Engineering. IGI Global, 2015. http://dx.doi.org/10.4018/978-1-4666-6363-3.ch013.

Full text

Abstract:

Tuberculosis (TB) is an airborne infectious disease caused by the bacterium Mycobacterium Tuberculosis (MTB) and is a persistent problem in developing countries. Present methods for its detection include normal or nested Polymerase Chain Reaction (PCR) followed by electrophoresis, real-time PCR, Ziehl-Neelsen staining, and culture assay. These techniques entail various disadvantages such as high cost, long assay time and use of toxic substances. Novel loop-mediated isothermal amplification (LAMP) permits DNA to be amplified rapidly under constant temperature. The combination of LAMP and chroma

APA, Harvard, Vancouver, ISO, and other styles

5

Ohkuma, Shoji. "[12] Use of fluorescein isothiocyanate-dextran to measure proton pumping in lysosomes and related organelles." In Biomembranes Part U: Cellular and Subcellular Transport: Eukaryotic (Nonepithelial) Cells. Elsevier, 1989. http://dx.doi.org/10.1016/0076-6879(89)74015-5.

Full text

APA, Harvard, Vancouver, ISO, and other styles

6

"A Histological Study of the Alimentary Tract of four African Fishes. Fluorescein Isothiocyanate Labelled (FITC) Lectins." In Proceedings of IUB Symposium No. 144, The Seventh International Lectin Meeting Bruxelles, Belgium, August 18–23, 1985. De Gruyter, 1986. http://dx.doi.org/10.1515/9783112315958-062.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Pollack, Alan. "Chapter 30 Flow Cytometric Cell-Kinetic Analysis by Simultaneously Staining Nuclei with Propidium Iodide and Fluorescein Isothiocyanate." In Flow Cytometry. Elsevier, 1990. http://dx.doi.org/10.1016/s0091-679x(08)60535-x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

"Fluoroscein-5-isothiocyanate (FITC)." In Handbook of Fluorescent Dyes and Probes. John Wiley & Sons, Inc, 2015. http://dx.doi.org/10.1002/9781119007104.ch80.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Karlish, S. J. D. "[24] Use of formycin nucleotides, intrinsic protein fluorescence, and fluorescein isothiocyanate-labeled enzymes for measurement of conformational states of Na+,K+-ATPase." In Methods in Enzymology. Elsevier, 1988. http://dx.doi.org/10.1016/0076-6879(88)56027-5.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

"7-Dimethylamino-4-methylcoumarin-3-isothiocyanate (DACITC)." In Handbook of Fluorescent Dyes and Probes. John Wiley & Sons, Inc, 2015. http://dx.doi.org/10.1002/9781119007104.ch69.

Full text

APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "Fluorescein isothiocyanate"

1

Dovichi, Norman J., Shade Wu, and Da Yung Chen. "High Sensitivity Fluorescence Detection of Biological Molecules." In Laser Applications to Chemical Analysis. Optica Publishing Group, 1990. http://dx.doi.org/10.1364/laca.1990.tha1.

Full text

Abstract:

Fluorescein is a good fluorescent label for high sensitivity analysis. The molecule has high molar absorptivity, 5 × 104 L mol-1 cm-1 at 488 nm and near unit fluorescence quantum yield in the pH range of 8 to 10. Unfortunately, the molecule is not photostable undergoing irreversible photobleaching after absorbance of about 8,000 photons. Fluorescein may be used to label amino groups in amino acids, peptides, and proteins through the isothiocyanate derivative. Under basic conditions, the thiocarbamoyl derivative is formed, with relatively good stability. The reaction between amino acids and flu

APA, Harvard, Vancouver, ISO, and other styles

2

Marinko, Katja, Luka Irenej Pečan, Julija Vodenik, et al. "Fluorescence and Fluorescein as Pivotal Tools in Cancer Diagnosis and Therapy." In Socratic Lectures 11. University of Lubljana Press, 2024. http://dx.doi.org/10.55295/psl.11.2024.2.

Full text

Abstract:

Fluorescent dyes have become important tools in various scientific and medical fields due to their unique capabilities for visualizing and analysing complex biological systems. This review focuses on the pivotal role of fluorescein and its derivatives in cancer diagnosis and therapy. Fluorescein, initially synthesized in the 19th century, has evolved into a crucial diagnostic and research tool, particularly valued for its intense fluorescence and versatility. In medical practice, fluorescein is used in high-contrast imaging techniques such as fluorescein angiography and fluorescence-guided sur

APA, Harvard, Vancouver, ISO, and other styles

3

Dastgheyb, Raha M., Michael C. Cochran, and Kenneth A. Barbee. "Interactions of fluorescein isothiocyanate-labeled poloxamer P188 with cultured cells." In 2012 38th Annual Northeast Bioengineering Conference (NEBEC). IEEE, 2012. http://dx.doi.org/10.1109/nebc.2012.6207089.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Deka, C., B. E. Lehnert, N. M. Lehnert, G. M. Jones, L. A. Sklar, and J. A. Steinkamp. "Rapid Analysis of Fluorescence Quenching of FITC Conjugated Antibodies on Individual Cells by Phase-Sensitive Flow Cytometry." In Biomedical Optical Spectroscopy and Diagnostics. Optica Publishing Group, 2006. http://dx.doi.org/10.1364/bosd.1996.ca1.

Full text

Abstract:

We demonstrate a high speed method to analyze the impact of self-quenching on fluorescence lifetimes in individual cells by phase-sensitive flow cytometry, using a model system consisting of FITC (fluorescein isothiocyanate) labeled antimouse Thy 1.2 antibodies bound to murine thymus cells.

APA, Harvard, Vancouver, ISO, and other styles

5

Biryukov, M. M., A. A. Polyakova, N. V. Kryachkova, et al. "PEG-COATED GOLD NANOPARTICLES AND PROSPECTS OF THEIR USE FOR ENHANCING COLD PLASMA CYTOTOXIC EFFECT AND FOR DRUG MOLECULES DELIVERY TO TUMOR CELLS." In X Международная конференция молодых ученых: биоинформатиков, биотехнологов, биофизиков, вирусологов и молекулярных биологов — 2023. Novosibirsk State University, 2023. http://dx.doi.org/10.25205/978-5-4437-1526-1-165.

Full text

Abstract:

Gold nanoparticles with diameters of 6 and 13 nm have been found to enhance the cytotoxic effect of cold plasma on human tumor cells. Additionally, cold plasma has been shown to promote the uptake of gold nanoparticles that are conjugated with fluorescein isothiocyanate by tumor cells. Gold nanoparticles can be utilized in conjunction with cold plasma to deliver drug molecules to cells.

APA, Harvard, Vancouver, ISO, and other styles

6

cummings, steven M. "Fluorescein Isothiocyanate (FITC) Labelled Dextran As A Peripheral Marker Of Lung Inflammation." In American Thoracic Society 2011 International Conference, May 13-18, 2011 • Denver Colorado. American Thoracic Society, 2011. http://dx.doi.org/10.1164/ajrccm-conference.2011.183.1_meetingabstracts.a4376.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Yan, Karen Chang, Michael Rossini, Michael Sebok, and John Sperduto. "Concentration Characterization of Encapsulated Macromolecules in Electrospun Alginate Fibers Using Image Analysis." In ASME 2015 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/imece2015-52585.

Full text

Abstract:

Electrospun fibers made of biocompatible polymers have been used as scaffolds in tissue engineering to mimic the fibrous environment found in the extracellular matrix (ECM) of biological tissue; and bioactive macromolecules can also be encapsulated in the electrospun fibers. In order to control the release of these encapsulated macromolecules, it is of great interest to understand how the release rate is affected by the sizes of molecules, cross-linking as well as electrospinning configuration (single axial versus co-axial). Fluorescein imaging technique has been applied in quantifying molecul

APA, Harvard, Vancouver, ISO, and other styles

8

Brewer, Bryson, Yandong Gao, and Deyu Li. "A Study of Small Molecule Absorption in Polydimethylsiloxane." In ASME 2012 Third International Conference on Micro/Nanoscale Heat and Mass Transfer. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/mnhmt2012-75105.

Full text

Abstract:

While polydimethylsiloxane (PDMS) has proven to be a popular material in the construction of microfluidic devices, the polymer network structure of PDMS makes it permeable to some molecules. This feature can be problematic for applications where thin PDMS walls are used to separate two different molecular streams or cell populations. Therefore, a better understanding of factors affecting small molecule absorption in PDMS is important for better design and operation of microfluidic devices. We report on studies of various factors in the absorption of fluorescent dyes in PDMS. Results show signi

APA, Harvard, Vancouver, ISO, and other styles

9

Hossan, Mohammad R., Talukder Z. Jubery, Danny R. Bottenus, Prashanta Dutta, Cornelius F. Ivory, and Wenji Dong. "Preconcentration of Cardiac Proteins in a Cascade Microchip." In ASME 2011 International Mechanical Engineering Congress and Exposition. ASMEDC, 2011. http://dx.doi.org/10.1115/imece2011-63812.

Full text

Abstract:

Concentration of bio-molecules prior to detection is very critical in the development of an integrated, multifunctional lab-on-a chip device for detection of ultra trace molecules from complex biological fluids such as serum, urine, or saliva. In this work, the preconcentration of a clinically relevant biomarker, cardiac troponin I (cTnI), is demonstrated in a cascade microfluidic channel using cationic isotachophoresis (ITP). The cascade chip is formed on PMMA (poly methyl methacrylate) with gradual changes in size both in width and depth direction to achieve a 100× reduction in overall cross

APA, Harvard, Vancouver, ISO, and other styles

10

Yang, Sung, Akif U¨ndar, and Jeffrey D. Zahn. "Continuous Particle Cross Over in Microfluidic Channels for Continuous Biosensing." In ASME 2006 International Mechanical Engineering Congress and Exposition. ASMEDC, 2006. http://dx.doi.org/10.1115/imece2006-16087.

Full text

Abstract:

A novel concept for continuous biosensing is introduced. Continuous biosensing is achieved by the mechanism named "Particle Cross Over" within microfluidic channels. The functionality of the device is demonstrated by quantifying the binding events of biotin to streptavidin coated beads. The device is designed to have precisely controlled flow structures which allow cytometric beads to pass from one flow region to another without mixing the carrier and sample fluids. The mean and standard deviation of the cytometric bead pixel intensities are 43.52 and 19.39, respectively for 1 μg/ml of biotiny

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!