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Journal articles on the topic 'Fluorescein isothiocyanate'

Author: Grafiati

Published: 24 April 2022

Last updated: 30 July 2025

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1

Spiguel, Lisa, Christiana Shaw, Adam Katz, et al. "Fluorescein Isothiocyanate." Annals of Plastic Surgery 78 (June 2017): S296—S298. http://dx.doi.org/10.1097/sap.0000000000001034.

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2

Kaewsaneha, Chariya, Kulachart Jangpatarapongsa, Tienrat Tangchaikeeree, Duangporn Polpanich, and Pramuan Tangboriboonrat. "Fluorescent chitosan functionalized magnetic polymeric nanoparticles: Cytotoxicity and in vitro evaluation of cellular uptake." Journal of Biomaterials Applications 29, no. 5 (2014): 761–68. http://dx.doi.org/10.1177/0885328214540349.

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Nanoparticles possessing magnetic and fluorescent properties were fabricated by the covalent attachment of fluorescein isothiocyanate onto magnetic polymeric nanoparticles functionalized by chitosan. The synthesized magnetic polymeric nanoparticles-chitosan/fluorescein isothiocyanate were successfully used for labeling the living organ and blood-related cancer cells, i.e., HeLa, Hep G2, and K562 cells. The cytotoxicity test of nanoparticles at various incubation times indicated the high cell viability (>90%) without morphological change. The confocal microscopy revealed that they could pass

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3

Chin, Suk Fun, Aressa Azman, Suh Cem Pang, and Sing Muk Ng. "Fluorescein-Labeled Starch Maleate Nanoparticles as Sensitive Fluorescent Sensing Probes for Metal Ions." Journal of Nanomaterials 2014 (2014): 1–8. http://dx.doi.org/10.1155/2014/108359.

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Fluorescein 5(6)-isothiocyanate starch maleate (FISM) nanoparticles were prepared by covalently attached fluorescein 5(6)-isothiocyanate (FITC) with starch maleate. FISM nanoparticles with a mean particle size of 87 nm were formed via self-assembly upon precipitation in ethanolic solution. FISM nanoparticles were strongly fluorescent with maximum emission wavelength of 518 nm. The fluorescence of FISM nanoparticles can be quenched by silver (Ag+) and lead (Pb2+) ions in a concentration dependent manner. We have demonstrated the first use of FISM nanoparticles as cheap and effective fluorescent

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4

Wang, Jing, Meng Ma, Rongbin Huang, Ligeng Wang, Aimin Chen, and Jun Hu. "An efficient ratiometric fluorescent probe based on dual-emission fluorescent silica nanoparticles for visual determination of Hg2+." Analytical Methods 7, no. 6 (2015): 2295–99. http://dx.doi.org/10.1039/c4ay02905d.

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5

Wiley, J. S., A. M. Brocklebank, M. B. Snook, et al. "A new fluorescent probe for the equilibrative inhibitor-sensitive nucleoside transporter. 5′-S-(2-aminoethyl)-N6-(4-nitrobenzyl)-5′-thioadenosine (SAENTA)-x2-fluorescein." Biochemical Journal 273, no. 3 (1991): 667–72. http://dx.doi.org/10.1042/bj2730667.

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The N6-(4-nitrobenzyl) derivative of adenosine is a tight-binding inhibitor of the equilibrative inhibitor-sensitive nucleoside transporter of mammalian cells. A fluorescent ligand for this transporter has been synthesized by allowing an adenosine analogue. 5′-S-(2-aminoethyl)-N6-(4-nitrobenzyl)-5′-thioadenosine (SAENTA), to react with fluorescein isothiocyanate. The purified adduct had a SAENTA/fluorescein molar ratio of 0.92:1 calculated from its absorption spectrum. The intensity of fluorescent emission from the SAENTA-chi 2-fluorescein adduct was 30% that of fluorescein isothiocyanate (chi

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6

Malatesti, Nela, Robert Hudson, Karen Smith, et al. "Isothiocyanato Boron Dipyrromethenes—The First BODIPY Analogues of Fluorescein Isothiocyanate." Photochemistry and Photobiology 82, no. 3 (2006): 746. http://dx.doi.org/10.1562/2005-01-10-ra-769.

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7

ADAMO, Hugo P., Adelaida G. FILOTEO, and John T. PENNISTON. "The plasma membrane Ca2+ pump mutant lysine591 → arginine retains some activity, but is still inactivated by fluorescein isothiocyanate." Biochemical Journal 317, no. 1 (1996): 41–44. http://dx.doi.org/10.1042/bj3170041.

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Inactivation of the wild-type human plasma membrane Ca2+ pump (isoform 4b) by fluorescein isothiocyanate is accompanied by covalent modification of Lys591. The mutation of Lys591 to arginine reduced the Ca2+ transport activity to 35% of the wild-type, and diminished the amount of acylphosphate formed from ATP by a corresponding amount. When this mutant was treated with fluorescein isothiocyanate, the enzyme was still irreversibly inactivated, even though no reactive residue was available at position 591. The results show that, although Ca2+ pump function is sensitive to the residue at position

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8

Pauls, K. P., and P. V. Chuong. "Flow cytometric identification of Brassica napus protoplast fusion products." Canadian Journal of Botany 65, no. 5 (1987): 834–38. http://dx.doi.org/10.1139/b87-113.

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A flow cytometric method to identify Brassica somatic hybrids has been developed. The procedure is based on the use of fluorescein isothiocyanate stained hypocotyl protoplasts and unstained mesophyll protoplasts as partners for polyethylene glycol induced protoplast fusion. The fluorescein isothiocyanate stained hypocotyl protoplasts and unstained mesophyll protoplasts were passed through the flow cytometer – cell sorter separately to maximize the sensitivity of the red and green detectors to chlorophyll and fluorescein isothiocyanate fluorescence, respectively, and to ensure that there was no

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9

Fratoddi, Ilaria, Chiara Battocchio, Giovanna Iucci, et al. "Silver Nanoparticles Functionalized by Fluorescein Isothiocyanate or Rhodamine B Isothiocyanate: Fluorescent and Plasmonic Materials." Applied Sciences 11, no. 6 (2021): 2472. http://dx.doi.org/10.3390/app11062472.

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This paper presents the synthesis of silver nanoparticles (AgNPs) functionalized with fluorescent molecules, in particular with xanthene-based dyes, i.e., fluorescein isothiocyanate (FITC, λmax = 485 nm) and rhodamine B isothiocyanate (RITC, λmax = 555 nm). An in-depth characterization of the particle–dye systems, i.e., AgNPs–RITC and AgNPs–FITC, is presented to evaluate their chemical structure and optical properties due to the interaction between their plasmonic and absorption properties. UV–Vis spectroscopy and the dynamic light scattering (DLS) measurements confirmed the nanosize of the Ag

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10

Gatto, C., and M. A. Milanick. "Inhibition of the red blood cell calcium pump by eosin and other fluorescein analogues." American Journal of Physiology-Cell Physiology 264, no. 6 (1993): C1577—C1586. http://dx.doi.org/10.1152/ajpcell.1993.264.6.c1577.

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This paper addresses the mechanism of inhibition of the plasma membrane Ca pump by fluorescein analogues and their isothiocyanate derivatives. Eosin (i.e., tetrabromofluorescein) was found to be one of the most potent reversible inhibitors of the erythrocyte Ca pump [half-maximal inhibitory concentration (IC50) < 0.2 microM]; fluorescein itself was about four orders of magnitude less potent (IC50 approximately 1,000 microM). Eosin decreased the maximum influx and thus did not compete with ATP for the Ca pump. Irreversible inhibition produced by the isothiocyanate analogues of eosin and fluo

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11

Jaklová Dytrtová, Jana, Karina Moslova, Michal Jakl, Heli Sirén, and Marja-Liisa Riekkola. "Fluorescein isothiocyanate stability in different solvents." Monatshefte für Chemie - Chemical Monthly 152, no. 11 (2021): 1299–306. http://dx.doi.org/10.1007/s00706-021-02852-1.

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12

Chu, Jia, Xin Li, Ying Li, and Limin Chang. "Coupled fluorescein isothiocyanate on graphene oxide." Materials Letters 65, no. 4 (2011): 751–53. http://dx.doi.org/10.1016/j.matlet.2010.12.001.

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13

Swoboda, Gertrude, and Wilhelm Hasselbach. "Reaction of Fluorescein Isothiocyanate with Thiol and Amino Groups of Sarcoplasmic ATPase." Zeitschrift für Naturforschung C 40, no. 11-12 (1985): 863–75. http://dx.doi.org/10.1515/znc-1985-11-1220.

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Abstract Several model compounds containing thiol and/or amino groups (mercaptoethanol, glutathione, cysteine, ethanolamine, glycine) were studied with respect to their reactivity towards fluorescein isothiocyanate (followed spectrophotometrically at 504 and 412 nm), stability of product and long wave absorption maximum of the fluorescein residue attached. Thiol groups reacted by far more readily than amino groups. A specific effect was observed with cysteine, indicating an intramolecular transfer of the fluorescein residue from SH to NH2.With sarcoplasmic vesicles both types of reactions wer

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14

Struck, C., R. Rohringer, and R. Heitefuss. "Isolation and lectin-binding properties of barley epidermal and mesophyll protoplasts." Canadian Journal of Botany 72, no. 11 (1994): 1688–91. http://dx.doi.org/10.1139/b94-207.

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Protoplasts from primary leaves of barley (Hordeum vulgare L.) were obtained by enzymatic digestion and fractionated by discontinuous density gradient centrifugation to yield highly enriched fractions of mesophyll and epidermal protoplasts. A characterization of both protoplast types resulted in a clear differentiation of the outer protoplast surfaces. The protoplasts were examined for affinity to various lectins by agglutination tests and by labeling with lectin – fluorescein isothiocyanate conjugates. Both types of protoplasts agglutinated with soybean lectin. Fluorescein isothiocyanate-labe

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15

MILLER, Larry, Martin PHILLIPS, and Emil REISLER. "Polymerization of actin modified with fluorescein isothiocyanate." European Journal of Biochemistry 174, no. 1 (1988): 23–29. http://dx.doi.org/10.1111/j.1432-1033.1988.tb14057.x.

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16

Casanovas, Jordi, Denis Jacquemin, Eric A. Perpète, and Carlos Alemán. "Fluorescein isothiocyanate: Molecular characterization by theoretical calculations." Chemical Physics 354, no. 1-3 (2008): 155–61. http://dx.doi.org/10.1016/j.chemphys.2008.10.008.

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17

Lin, Y. C., C. H. Ho, and F. Grinnell. "Fibroblasts contracting collagen matrices form transient plasma membrane passages through which the cells take up fluorescein isothiocyanate-dextran and Ca2+." Molecular Biology of the Cell 8, no. 1 (1997): 59–71. http://dx.doi.org/10.1091/mbc.8.1.59.

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When fibroblasts contract collagen matrices, the cells activate a Ca(2+)-dependent cyclic AMP signaling pathway. We have found that contraction also stimulates uptake of fluorescein isothiocyanate-dextran molecules from the medium. Our results indicate that fluorescein isothiocyanate-dextran enters directly into the cell cytoplasm through 3- to 5-nm plasma membrane passages. These passages, which reseal in less than 5 s in the presence of divalent cations, also are likely sites of Ca2+ uptake during contraction and the first step in contraction-activated cyclic AMP signaling. The formation of

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18

Cao, Feng, Yinan Li, Jiahao Wu, Wei Liu, and To Ngai. "Measurements of interactions between fluorescent molecules and polyethylene glycol self-assembled monolayers." Soft Matter 18, no. 1 (2022): 236–43. http://dx.doi.org/10.1039/d1sm01329g.

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19

Zeng, Haijuan, Xuzhao Zhai, Manman Xie, and Qing Liu. "Fluorescein Isothiocyanate Labeling Antigen-Based Immunoassay Strip for Rapid Detection of Acidovorax citrulli." Plant Disease 102, no. 3 (2018): 527–32. http://dx.doi.org/10.1094/pdis-06-17-0903-re.

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A simple and fast immunoassay strip to detect Acidovorax citrulli (Ac) using fluorescein isothiocyanate as a marker was developed. Fluorescein isothiocyanate (FITC) was added to sample culture medium for bacteria incubation, and the bacteria could emit a yellow-green fluorescence under ultraviolet light and become a fluorescent probe. This immunofluorescence strip (IFS) was based on the binding between fluorescent bacteria and the unlabeled monoclonal antibody (McAb) immobilized on the test area in nitrocellulose membrane. The detection limit of the strip was 106 CFU/ml with a result that coul

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20

Appiah-Ntiamoah, Richard, Bekelcha Tesfaye Gadisa, and Herm Kim. "An effective electrochemical sensing platform for fluoride ions based on fluorescein isothiocyanate–MWCNT composite." New Journal of Chemistry 42, no. 14 (2018): 11341–50. http://dx.doi.org/10.1039/c8nj01703d.

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21

Moreira, Josino C., Chun-Fat J. Law, and Arnold G. Fogg. "Adsorptive stripping voltammetry of fluorescein isothiocyanate, phenyl isothiocyanate, phenylthiohydantoin-tyrosine and methylthiohydantoin-glycine." Analyst 114, no. 12 (1989): 1607. http://dx.doi.org/10.1039/an9891401607.

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22

Shiba, K., M. Tagaya, T. Sugiyama, and N. Hanagata. "Preparation of luminescent titania/dye hybrid nanoparticles and their dissolution properties for controlling cellular environments." RSC Advances 5, no. 126 (2015): 104343–53. http://dx.doi.org/10.1039/c5ra23026h.

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Monodispersed titania/octadecylamine/fluorescein-isothiocyanate hybrid nanoparticles are synthesized to demonstrate a proof-of-concept for nanomedicines: an indirect molecular delivery system with no cytotoxicity.

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23

COLE, LOUISE, JULLIAN COLEMAN, DAVID EVANS, and CHRIS HAWES. "Internalisation of fluorescein isothiocyanate and fluorescein isothiocyanatedextran by suspension-cultured plant cells." Journal of Cell Science 96, no. 4 (1990): 721–30. http://dx.doi.org/10.1242/jcs.96.4.721.

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The uptake of pure non-conjugated fluorescein isothiocyanate (FITC) and of the membraneimpermeant probe FITC—dextran into suspensioncultured carrot cells and protoplasts has been investigated. Commercial samples of a 70K (K=103Mr) FITC—dextran were shown to contain contaminant FITC and/or its degradation products, which were rapidly internalised into the vacuolar system of both cells and protoplasts. However, purified samples of the 70K FITC—dextran were taken up into the vacuoles of cells but not protoplasts after a lh incubation period. This apparent difference in the ability of cells and pr

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24

McKenna, N., J. B. Meigs, and Y. L. Wang. "Identical distribution of fluorescently labeled brain and muscle actins in living cardiac fibroblasts and myocytes." Journal of Cell Biology 100, no. 1 (1985): 292–96. http://dx.doi.org/10.1083/jcb.100.1.292.

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We have investigated whether living muscle and nonmuscle cells can discriminate between microinjected muscle and nonmuscle actins. Muscle actin purified from rabbit back and leg muscles and labeled with fluorescein isothiocyanate, and nonmuscle actin purified from lamb brain and labeled with lissamine rhodamine B sulfonyl chloride, were co-injected into chick embryonic cardiac myocytes and fibroblasts. When fluorescence images of the two actins were compared using filter sets selective for either fluorescein isothiocyanate or lissamine rhodamine B sulfonyl chloride, essentially identical patte

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25

Shah, Zahoor A., Rung-chi Li, Abdullah S. Ahmad, et al. "The Flavanol (−)-Epicatechin Prevents Stroke Damage through the Nrf2/HO1 Pathway." Journal of Cerebral Blood Flow & Metabolism 34, no. 4 (2014): 735. http://dx.doi.org/10.1038/jcbfm.2014.10.

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Correction to: Journal of Cerebral Blood Flow & Metabolism (2010) 30, 1951–1961; doi: 10.1038/jcbfm.2010.53 After the publication of this article, the following errors in the Materials and Methods section were noticed: On page 1953 in the Materials and Methods section it is erroneously stated that ‘Cells were washed and incubated with rhodamine-conjugated, affinity-purified donkey anti-rat IgG (H+L) and fluorescein isothiocyanate (FITC)-conjugated, affinity-purified goat anti-rabbit IfG (H+L)’. Correction: Cells were washed and incubated with rhodamine-conjugated, affinity-purified donkey

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26

Appiah-Ntiamoah, Richard, Bekelcha Tesfaye Gadisa, and Hern Kim. "Correction: An effective electrochemical sensing platform for fluoride ions based on fluorescein isothiocyanate–MWCNT composite." New Journal of Chemistry 42, no. 14 (2018): 12263. http://dx.doi.org/10.1039/c8nj90059k.

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Correction for ‘An effective electrochemical sensing platform for fluoride ions based on fluorescein isothiocyanate–MWCNT composite’ by Richard Appiah-Ntiamoah et al., New J. Chem., 2018, DOI: 10.1039/c8nj01703d.

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27

LONERGAN, S. M., M. H. JOHNSON, and C. R. CALKINS. "Improved Calpain Assay Using Fluorescein Isothiocyanate-Labeled Casein." Journal of Food Science 60, no. 1 (1995): 72–73. http://dx.doi.org/10.1111/j.1365-2621.1995.tb05609.x.

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28

Rath, N. C., G. R. Huff, J. M. Balog, and W. E. Huff. "Fluorescein isothiocyanate staining and characterization of avian heterophils." Veterinary Immunology and Immunopathology 64, no. 1 (1998): 83–95. http://dx.doi.org/10.1016/s0165-2427(98)00122-6.

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29

López-Zabalza, María J., Susana Sanz, Ana Iriarte, Natalia López-Moratalla, and E. Santiago. "Modification of skeletal S-1 with fluorescein isothiocyanate." Comparative Biochemistry and Physiology Part B: Comparative Biochemistry 96, no. 1 (1990): 101–5. http://dx.doi.org/10.1016/0305-0491(90)90348-w.

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30

Kim, Hee Cheol, and Won Ho Park. "Fluorescent property of glycol chitosan-fluorescein isothiocyanate conjugate for bio-imaging material." International Journal of Biological Macromolecules 135 (August 2019): 1217–21. http://dx.doi.org/10.1016/j.ijbiomac.2019.06.038.

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31

Dai, Li, Wenjun Wang, Jie Yan, and Yong Liu. "Novel Synthesis of Fluorescein Isothiocyanate-Based Fluorescent Nanoprobes in Imaging Lung Inflammation." Journal of Biomedical Nanotechnology 20, no. 4 (2024): 615–27. http://dx.doi.org/10.1166/jbn.2024.3795.

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We aimed to examine the novel synthesis of fluorescent nanoprobes synthesized in imaging lung inflammation and diseased tissues. All reagents were purchased from commercial suppliers to synthesize the PLGA, PEG, GFP, RFP, rhodamine, and magnetic fluorescent nanoprobes. We performed experiments using human lung cells from the Chinese Academy of Medical Sciences Cell Center. The cells were cultured in a DMEM medium. Confocal microscopy was used to label the cells during imaging. All statistical analyses were performed in GraphPad Prism. There were significant differences in the fluorescent inten

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32

Gao, X. P., and I. Rubinstein. "The stable VIP analogue, Ro 24-9981, potentiates bradykinin-induced increases in clearance of macromolecules." American Journal of Physiology-Heart and Circulatory Physiology 269, no. 5 (1995): H1648—H1655. http://dx.doi.org/10.1152/ajpheart.1995.269.5.h1648.

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The purpose of this study was to determine whether the stable cyclic peptide analogue of human vasoactive intestinal peptide, Ro 24-9981, modulates bradykinin-induced plasma exudation in the oral mucosa and if so, to determine the mechanisms that mediated these responses. Using intravital microscopy, we found that suffusion of Ro 24-9981 had no significant effects on leaky site formation and clearance of fluorescein-isothiocyanate-dextran (mol mass 70 kDa) in the hamster cheek pouch. However, Ro 24-9981 significantly potentiated bradykinin-induced increases in leaky site formation and clearanc

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33

Bertelà, Federica, Chiara Battocchio, Giovanna Iucci, et al. "Dye-Doped Polymeric Microplastics: Light Tools for Bioimaging in Test Organisms." Polymers 15, no. 15 (2023): 3245. http://dx.doi.org/10.3390/polym15153245.

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Ecosystems around the world are experiencing a major environmental impact from microplastic particles (MPs 0.1 µm–1 mm). Water, sediments, and aquatic biota show the widespread presence of this pollutant. However, MPs are rarely used in laboratory studies as they are scarcely available for purchase or expensive, especially if one wishes to trace the particle with a dye or fluorescent. Furthermore, existing preparation techniques have limited application in biological studies. In this work, we propose a new, easy, and cheap way to prepare fluorescent MPs. The protocol is based on the osmosis me

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34

Hulspas, R., P. J. Krijtenburg, J. F. Keij, and J. G. Bauman. "Avidin-EITC: an alternative to avidin-FITC in confocal scanning laser microscopy." Journal of Histochemistry & Cytochemistry 41, no. 8 (1993): 1267–72. http://dx.doi.org/10.1177/41.8.7687265.

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Detection of fluorescein-5-isothiocyanate (FITC)-labeled conjugates is suboptimal in two-color confocal scanning laser microscopy (CLSM). This limits the detection of small, dimly fluorescent targets. We explored the possible advantages of applying eosin-5-isothiocyanate (EITC) conjugated to avidin (Av-EITC) as an alternative for Av-FITC in CSLM. Despite the lower quantum efficiency of EITC, we found that the measured Av-EITC and Av-FITC emission intensities were similar as a result of the standard filter combinations used for simultaneous two-color detection in the Bio-Rad MRC 600 CSLM. The a

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35

Mao, Kang, Yizhen Liu, Huaming Xiao, et al. "A novel platform for detection of protooncogene based on Au nanocluster enhanced fluorescence." Analytical Methods 7, no. 1 (2015): 40–44. http://dx.doi.org/10.1039/c4ay02117g.

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For the first time, gold nanoclusters were found to exhibit high fluorescence enhancement ability based on the metal-enhanced fluorescence (MEF) effect, which can effectively enhance the fluorescence of fluorescein isothiocyanate (FITC).

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36

Davidson, R. S., and J. E. Pratt. "The effects of enzymes on the photobleaching of fluorescein and fluorescein isothiocyanate conjugates." Journal of Photochemistry and Photobiology B: Biology 1, no. 3 (1988): 361–69. http://dx.doi.org/10.1016/1011-1344(88)85023-1.

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37

Johansson, Maria C., Bo Baldetorp, and Stina M. Oredsson. "Energy Transfer between Fluorescein Isothiocyanate and Propidium Iodide – A Problem in the Estimation of Tpotwith the Bromodeoxyuridine–DNA Flow Cytometry Technique?" Analytical Cellular Pathology 19, no. 2 (1999): 91–98. http://dx.doi.org/10.1155/1999/364846.

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Energy transfer in flow cytometry can occur when two fluorochromes are bound in close proximity (generally within 100 Å) and the emission spectrum of one fluorochrome overlaps significantly with the excitation spectrum of the other. The latter criterium is fullfilled for the fluorochromes fluorescein isothiocyanate and propidium iodide and also the former when they, e.g., are used in bromodeoxyuridine – DNA flow cytometry methods. In the present growth kinetic study using this method, we show that energy transfer does take place between fluorescein isothiocyanate and propidium iodide which res

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38

Hormozi-Nezhad, M. Reza, and M. Taghipour. "An ultrasensitive and selective turn-off fluorescent nanoprobe for the detection of copper ions." Analytical Methods 7, no. 12 (2015): 5067–73. http://dx.doi.org/10.1039/c5ay00766f.

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In this study, a novel approach for sensitive and extremely selective detection of copper ions has been developed based on fluorescence resonance energy transfer (FRET) between AuNPs as an acceptor and fluorescein isothiocyanate (FITC) as a donor.

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39

Chang, Shu-Hao, Yu-Tung Tsai, Guo-An Li, Shao-Lou Jheng, Tzu-Lun Kao, and Hsing-Yu Tuan. "Uniform silica coating of isoprene-passivated germanium nanowires via Stöber method." RSC Adv. 4, no. 76 (2014): 40146–51. http://dx.doi.org/10.1039/c4ra04858j.

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This paper describes a solution-based Stöber method for the coating of Ge nanowires (NWs) with a uniform thickness-tunable shell of amorphous silica. Fluorescein isothiocyanate (FITC) incorporated on the Ge–silica core–shell structure was demonstrated.

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40

Martin, Gottfried, Hansjürgen T. Agostini, and Lutz L. Hansen. "Light emitting diode microscope illumination for green fluorescent protein or fluorescein isothiocyanate epifluorescence." BioTechniques 38, no. 2 (2005): 204–6. http://dx.doi.org/10.2144/05382bm06.

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41

Glushko, V. N., L. I. Blokhina, and N. Yu Sadovskaya. "Studies on the synthesis of fluorescein-5-isothiocyanate: A fluorescent nanomarker for biosensors." Russian Journal of General Chemistry 85, no. 10 (2015): 2458–64. http://dx.doi.org/10.1134/s1070363215100412.

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42

Devi, Sarita, Rajwinder Kaur, Bhupender Singh, Ashok K. Paul, and Sachin Tyagi. "Fluorescent Sensing Assay for Trinitrotoluene Using Fluorescein Isothiocyanate Conjugated Mesoporous MCM-41 Particles." Journal of Nanoscience and Nanotechnology 18, no. 10 (2018): 6838–49. http://dx.doi.org/10.1166/jnn.2018.15456.

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43

Lai, Syu-Ming, Tsiao-Yu Tsai, Chia-Yen Hsu, Jai-Lin Tsai, Ming-Yuan Liao, and Ping-Shan Lai. "Bifunctional Silica-Coated Superparamagnetic FePt Nanoparticles for Fluorescence/MR Dual Imaging." Journal of Nanomaterials 2012 (2012): 1–7. http://dx.doi.org/10.1155/2012/631584.

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Recently, superparamagnetic chemically disordered face-centered cubic (fcc) FePt nanoparticles have been demonstrated as superior negative contrast agents for magnetic resonance imaging (MRI). However, their low intracellular labeling efficiency has limited the potential usage and the nanotoxicity of the particles requires attention. We have developed fluorescein isothiocyanate-incorporated silica-coated FePt (FePt@SiO2-FITC) nanoparticles that exhibited not only a significantT1andT2MR contrast abilities but also a fluorescent property without significant cytotoxicities. These results suggest

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44

Kaźmierczak, A., and J. Maszewski. "Uptake of fluorescent-labeled BSA into root cells: endocytosis?" Acta Societatis Botanicorum Poloniae 66, no. 3-4 (2014): 319–24. http://dx.doi.org/10.5586/asbp.1997.037.

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Incorporation of rhodamine- and fluorescein-isothiocyanate labeled bovine serum albumin (BSA-TRITC, BSA-FITC) was examined in different root zones of the 3-day-old seedlings in <em>Melandrium noctiflorum</em>, <em>Allium cepa</em> and <em>Zea mays</em>. The uptake of fluorescent-labeled BSA was found: (1) species-specific, (2) cell-type dependent, and (3) cytochalasin B-sensitive. The characteristic punctute distribution of vesicles within the cytoplasm suggests the internalization of labeled proteins by endocytosis.

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Caprifico, Anna E., Elena Polycarpou, Peter J. S. Foot, and Gianpiero Calabrese. "Fluorescein Isothiocyanate Chitosan Nanoparticles in Oral Drug Delivery Studies." Trends in Pharmacological Sciences 41, no. 10 (2020): 686–89. http://dx.doi.org/10.1016/j.tips.2020.07.005.

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Sari, Müfrettin Murat. "Fluorescein isothiocyanate conjugated graphene oxide for detection of dopamine." Materials Chemistry and Physics 138, no. 2-3 (2013): 843–49. http://dx.doi.org/10.1016/j.matchemphys.2012.12.069.

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ZHANG, Nian-Chun, Yan-Hong GAO, Chui-Ming WAN, and Ying-Liang LIU. "Preparation and Application of Fluorescein Isothiocyanate Fluorophore Nano-composites." Chinese Journal of Analytical Chemistry 38, no. 2 (2010): 202–6. http://dx.doi.org/10.1016/s1872-2040(09)60039-6.

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Bellelli, A., R. Ippoliti, M. Brunori, et al. "Binding and internalization of ricin labelled with fluorescein isothiocyanate." Biochemical and Biophysical Research Communications 169, no. 2 (1990): 602–9. http://dx.doi.org/10.1016/0006-291x(90)90373-u.

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SEO, K. H., and J. F. FRANK. "Attachment of Escherichia coli O157:H7 to Lettuce Leaf Surface and Bacterial Viability in Response to Chlorine Treatment as Demonstrated by Using Confocal Scanning Laser Microscopy." Journal of Food Protection 62, no. 1 (1999): 3–9. http://dx.doi.org/10.4315/0362-028x-62.1.3.

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Confocal scanning laser microscopy was used to observe the location of Escherichia coli O157:H7 on and within lettuce leaves. Sections of leaves (ca. 0.5 by 0.5 cm) were inoculated by submersion in a suspension of E. coli O157:H7 (ca. 107to 108CFU/ml) overnight at 7°C. Fluorescein isothiocyanate–labeled antibody was used to visualize the attached bacteria. E. coli O157:H7 was found attached to the surface, trichomes, stomata, and cut edges. Three-dimensional volume reconstruction of interior portions of leaves showed that E. coli O157:H7 was entrapped 20 to 100 μm below the surface in stomata

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Icenhour, Crystal R., Theodore J. Kottom, and Andrew H. Limper. "Evidence for a Melanin Cell Wall Component in Pneumocystis carinii." Infection and Immunity 71, no. 9 (2003): 5360–63. http://dx.doi.org/10.1128/iai.71.9.5360-5363.2003.

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ABSTRACT Fluorescein isothiocyanate-labeled monoclonal antibodies specific for fungal melanin were used in this study to visualize melanin-like components of the Pneumocystis carinii cell wall. A colorimetric enzyme assay confirmed these findings. This is the first report of melanin-like pigments in Pneumocystis.

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