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Journal articles on the topic 'Higenamine'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Shi, Hanghao, Long Cheng, Huixin Li, et al. "A Narrative Review on Higenamine: Pharmacological Properties and Clinical Applications." Nutrients 17, no. 6 (2025): 1030. https://doi.org/10.3390/nu17061030.

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Background: Higenamine, a bioactive alkaloid derived from plants such as Aconitum and Annona squamosa, has been traditionally used in Chinese medicine for treating heart diseases like bradycardia, arrhythmia, and heart failure. It exhibits multiple pharmacological effects, including anti-oxidative stress, improved cellular energy metabolism, anti-apoptosis, and enhanced erectile dysfunction. Aim and Methods: To investigate the reasons for these functions of higenamine and its application in the clinic, the literature of the database was searched and read in this study. Results: As a non-select

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2

Geng, Chao, та Shaowu Ou. "Higenamine Induces Glioma Cell Death by Modulating Nuclear Factor-κB Nuclear Translocation, Phosphoinositide-3-Kinase/Protein Kinase B Signaling and Caspase Cascade". Current Topics in Nutraceutical Research 19, № 3 (2020): 317–25. http://dx.doi.org/10.37290/ctnr2641-452x.19:317-325.

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We have investigated the effectiveness of higenamine in the treatment of malignant glioma, and explored its possible mechanism in C6 glioma cells. The efficacy of higenamine on viability of cells, apoptosis, cell cycle arrest, DNA fragmentation, and biochemical markers was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry, enzyme-linked immunosorbent assay, and Western blotting. The biochemical markers investigated included the effect of higenamine on the expression of phosphoinositide-3-kinase/protein kinase B, B-cell lymphoma 2, BCL2- associated X

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3

Nuntawong, Poomraphie, Hiroyuki Tanaka, Seiichi Sakamoto, and Satoshi Morimoto. "ELISA for the Detection of the Prohibited Doping Agent Higenamine." Planta Medica 86, no. 11 (2020): 760–66. http://dx.doi.org/10.1055/a-1181-2084.

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AbstractHigenamine is a natural benzyltetrahydroisoquinoline alkaloid produced by various plants. In the World Anti-Doping Agency report of 2020, higenamine is classified as a class S3 (selective and nonselective β2-agonist) prohibited substance. To minimize the problems resulting from the misuse of higenamine-containing products as well as from the abuse of doping agents in sport, numerous higenamine-detection methods have been investigated. In the present study, a monoclonal antibody against the (S)-enantiomer of higenamine was successfully produced and applied in the indirect competitive EL

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4

Rangelov Kozhuharov, Vanya, Kalin Ivanov, and Stanislava Ivanova. "Higenamine in Plants as a Source of Unintentional Doping." Plants 11, no. 3 (2022): 354. http://dx.doi.org/10.3390/plants11030354.

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Background: Higenamine is a β2 agonist of plant origin. The compound has been included in WADA’s prohibited list since 2017. Higenamine may be detected in different plants and many food supplements of natural origin. Methods: Our literature search was conducted through PubMed, Science Direct, Google Scholar, and Web of Science studies investigating the presence of higenamine in plants that are used in traditional folk medicine or included in food supplements. Our study aimed to assess the risk of adverse analytical findings caused by higenamine-containing plants. Results: Based on our literatu

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5

Xie, Yulu, Xican Li, Jingyu Chen, Yuman Deng, Wenbiao Lu, and Dongfeng Chen. "pH Effect and Chemical Mechanisms of Antioxidant Higenamine." Molecules 23, no. 9 (2018): 2176. http://dx.doi.org/10.3390/molecules23092176.

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In this article, we determine the pH effect and chemical mechanism of antioxidant higenamine by using four spectrophotometric assays: (1) 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide radical (PTIO•)-scavenging assay (at pH 4.5, 6.0, and 7.4); (2) Fe3+-reducing power assay; (3) Cu2+-reducing power assay; and (4) 1,1-diphenyl-2-picryl-hydrazyl (DPPH•)-scavenging assay. The DPPH•-scavenging reaction product is further analyzed by ultra-performance liquid chromatography, coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-Q-TOF-MS/MS) technol

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6

Shailaja, Amol D., and Jaykumar S. Pramodkumar. "Isolation, In-silico Studies, and Biological Evaluation of Higenamine from Annona squamosa L. against Breast Cancer." INTERNATIONAL JOURNAL OF PHARMACEUTICAL QUALITY ASSURANCE 14, no. 04 (2023): 1039–47. http://dx.doi.org/10.25258/ijpqa.14.4.34.

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The efficacy of Annona squamosa L. in cancer treatment has been documented, inspiring the authors to investigate the plant further for potential novel anticancer compounds. Therefore, the current study aimed to isolate and characterize Higenamine from leaves extract of A. squamosa L. and studied for the in-vitro cancer cell line and molecular docking. The high performance liquid chromatography (HPLC) and thin layer chromatography (TLC) method development of A. squamosa L. leaves extract furnished Higenamine of which characterization was established by HPLC, infrared radiation (IR), liquid chro

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7

Kim, DongHyun, JeaHyeok Yun, Eunmiri Roh, Han-Seung Shin, and Jong-Eun Kim. "Higenamine Reduces Fine-Dust-Induced Matrix Metalloproteinase (MMP)-1 in Human Keratinocytes." Plants 12, no. 13 (2023): 2479. http://dx.doi.org/10.3390/plants12132479.

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Environmental pollutants such as fine dust are increasingly linked to premature skin aging. In this study, we investigated the protective effects of higenamine, a natural plant alkaloid, against fine-dust-induced skin aging in human keratinocytes (HaCaT cells). We found that higenamine significantly attenuated fine-dust-induced expression of matrix metalloproteinase-1 (MMP-1), a key enzyme involved in collagen degradation. Furthermore, higenamine was found to modulate fine-dust-induced AP-1 and NF-κB transactivation, which are crucial factors for MMP-1 transcription. Higenamine also impeded fi

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8

Yen, Ching-Chi, Chun-Wei Tung, Chih-Wei Chang, Chin-Chuan Tsai, Mei-Chich Hsu, and Yu-Tse Wu. "Potential Risk of Higenamine Misuse in Sports: Evaluation of Lotus Plumule Extract Products and a Human Study." Nutrients 12, no. 2 (2020): 285. http://dx.doi.org/10.3390/nu12020285.

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Since 2017, higenamine has been added to the World Anti-Doping Agency (WADA) prohibited list as a β2-agonist prohibited at all times for sportspersons. According to WADA’s report, positive cases of higenamine misuse have been increasing yearly. However, higenamine occurs naturally in the Chinese herb lotus plumule—the green embryo of lotus (Nelumbo nucifera Gaertn) seeds—commercially available as concentrated powder on the Asian market. This study evaluated the major phytochemical components of lotus plumule products using an appropriate extraction method, followed by a human study in which th

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9

Chen, Yan-Ling, Xiao-Dong Zhuang, Zhi-Wei Xu, et al. "Higenamine Combined with [6]-Gingerol Suppresses Doxorubicin-Triggered Oxidative Stress and Apoptosis in Cardiomyocytes via Upregulation of PI3K/Akt Pathway." Evidence-Based Complementary and Alternative Medicine 2013 (2013): 1–14. http://dx.doi.org/10.1155/2013/970490.

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Sini decoction is a well-known formula of traditional Chinese medicine, which has been used to treat cardiovascular disease for many years. Previously, we demonstrated that Sini decoction prevented doxorubicin-induced heart failure in vivo. However, its active components are still unclear. Thus, we investigated the active components of Sini decoction and their cardioprotective mechanisms in the in vitro neonatal rat cardiomyocytes and H9c2 cell line models of doxorubicin-induced cytotoxicity. Our results demonstrated that treatment with higenamine or [6]-gingerol increased viability of doxorub

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10

Bloomer, RJ, JM Schriefer, and TA Gunnels. "Clinical safety assessment of oral higenamine supplementation in healthy, young men." Human & Experimental Toxicology 34, no. 10 (2015): 935–45. http://dx.doi.org/10.1177/0960327114565490.

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Objective: Higenamine, an herbal agent also known as norcoclaurine, is thought to stimulate β-androgenic receptors and possess lipolytic activity. It is currently making its way into the dietary supplement market. To our knowledge, no studies have been conducted to determine the safety profile of oral higenamine when used alone and in conjunction with other commonly used lipolytic agents. Methods: Forty-eight men were assigned to ingest either a placebo, higenamine, caffeine, or higenamine + caffeine + yohimbe bark extract daily for a period of 8 weeks. Before and after 4 and 8 weeks of supple

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11

Romeo, Isabella, Angela Parise, Annia Galano, Nino Russo, Juan Raúl Alvarez-Idaboy, and Tiziana Marino. "The Antioxidant Capability of Higenamine: Insights from Theory." Antioxidants 9, no. 5 (2020): 358. http://dx.doi.org/10.3390/antiox9050358.

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Density functional theory was employed to highlight the antioxidant working mechanism of higenamine in aqueous and lipid-like environments. Different reaction mechanisms were considered for the reaction of higenamine with the •OOH radical. The pH values and the molar fraction at physiological pH were determined in aqueous solution. The results show that the preferred reaction mechanism was the hydrogen atom transfer from the catecholic ring. The computed kinetic constants revealed that, in order to obtain reliable results, it is important to consider all the species present in water solution d

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12

Chang, Ki Churl, Won Seog Chong, and In Je Lee. "Different pharmacological characteristics of structurally similar benzylisoquinoline analogs, papaverine, higenamine, and GS 389, on isolated rat aorta and heart." Canadian Journal of Physiology and Pharmacology 72, no. 4 (1994): 327–34. http://dx.doi.org/10.1139/y94-049.

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In the present study, we compared the pharmacological properties of structurally similar benzylisoquinoline compounds, papaverine, higenamine, and GS 389, using isolated rat aorta and atrial preparations. The three benzylisoquinoline compounds, concentration dependently, relaxed phenylephrine (3 μM) induced contraction of rat aortic rings, with the rank order of potency being higenamine > papaverine > GS 389. They also relaxed high K+ (60 mM) induced contraction, with the rank order of potency being papaverine > GS 389 [Formula: see text] higenamine. The relaxation was not modified by

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13

Chen, Yanmin, Bujing Guo, Hongda Zhang, Lihong Hu та Jue Wang. "Higenamine, a Dual Agonist for β 1- and β 2-Adrenergic Receptors Identified by Screening a Traditional Chinese Medicine Library". Planta Medica 85, № 09/10 (2019): 738–44. http://dx.doi.org/10.1055/a-0942-4502.

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AbstractChronic heart failure is the terminal stage of various cardiovascular diseases. Despite the availability of several classes of drugs, there is still an unmet need for effective treatment. Based on bench work during the past two decades, we have proposed that enhancement of β 2-adrenergic receptor signaling in combination with the presently preferred β 1-adrenergic receptor blockade would be a promising strategy. Chinese herbal medicines have been shown to be effective in the treatment of heart failure, although the mechanisms largely remain unknown. In the present study, we screened an

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14

Wu, Dezhu, Zheng Zhang, Baoling Chen, and Ginfu Li. "Synthesis of [3H]-higenamine." Journal of Radioanalytical and Nuclear Chemistry 258, no. 3 (2003): 689–91. http://dx.doi.org/10.1023/b:jrnc.0000011772.32047.e5.

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15

Lin, Yi-An, Ching-Hsuang Hsu, Chin-Chuan Tsai, and Mei-Chich Hsu. "Determination Of Urinary Higenamine Excretion After Higenamine-containing Chinese Herbal Medicine Administration." Medicine & Science in Sports & Exercise 56, no. 10S (2024): 498. http://dx.doi.org/10.1249/01.mss.0001056896.61154.0f.

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16

Rangelov Kozhuharov, Vanya, Stanislava Ivanova, Vanya Nalbantova, Niko Benbassat та Kalin Ivanov. "Development and validation of an LC-PDA method for detection of the natural β2 agonist higenamine". Pharmacia 72 (21 травня 2025): 1–7. https://doi.org/10.3897/pharmacia.72.e151153.

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Higenamine is a natural β2 agonist that can be found in the extracts of many plants, including Nelumbo nucifera Gaertn., Tinospora crispa L., Nandina domestica Thunb., Gnetum parvifolium Warb., Asarum sieboldii Miq., Asarum heterotropoides F. Schmidt, Aconitum carmichaelii Debeaux, and Aristolochia brasiliensis Mart. & Zucc. Since 2017, the compound has been included in WADA’s prohibited list, and its detection in the biological samples of professional athletes is regarded as a violation of the anti-doping rules. Currently, many dietary supplements (DSs) contain plants that are rich in hig

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17

Yun-Choi, Hye Sook, Mi Kyung Pyo, Kyung Mi Park, Ki Churl Chang, and Duck Hyung Lee. "Anti-Thrombotic Effects of Higenamine." Planta Medica 67, no. 7 (2001): 619–22. http://dx.doi.org/10.1055/s-2001-17361.

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18

LO, CHI-FANG, and CHI-MING CHEN. "PHARMACOKINETICS OF HIGENAMINE IN RABBITS." Biopharmaceutics & Drug Disposition 17, no. 9 (1996): 791–803. http://dx.doi.org/10.1002/(sici)1099-081x(199612)17:9<791::aid-bdd993>3.0.co;2-t.

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19

Huang, Ting, Zhong-Hua Dai, Fei Long, Yu-Tian Lei, Mao-Hua Yuan, and Gui-Hua Jiang. "Identification of key bioactive anti-migraine constituents of Asari radix et rhizoma using network pharmacology and nitroglycerin-induced migraine rat model." Tropical Journal of Pharmaceutical Research 20, no. 5 (2022): 987–94. http://dx.doi.org/10.4314/tjpr.v20i5.15.

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    Purpose: To elucidate the bioactive constituents of Asari radix et rhizoma (ARR) in treating migraine based on network pharmacology and nitroglycerin-induced migraine rat model. Methods: The potential bioactive constituents of ARR were identified with the aid of literature retrieval and virtual screening, and the migraine-related hub genes were identified using protein-protein interaction and topology analyses. Then, the interaction between the potential bioactive constituents and hub genes was determined with molecular docking and topology, leading to the pre

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20

Rangelov, Kozhuharov Vanya, Stanislava Ivanova, Vanya Nalbantova, Niko Benbassat та Kalin Ivanov. "Development and validation of an LC-PDA method for detection of the natural β2 agonist higenamine". Pharmacia 72 (21 травня 2025): 1–7. https://doi.org/10.3897/pharmacia.72.e151153.

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Higenamine is a natural <i>β<sub>2</sub></i> agonist that can be found in the extracts of many plants, including <i>Nelumbo nucifera</i> Gaertn., <i>Tinospora crispa</i> L., <i>Nandina domestica</i> Thunb., <i>Gnetum parvifolium</i> Warb., <i>Asarum sieboldii</i> Miq., <i>Asarum heterotropoides</i> F. Schmidt, <i>Aconitum carmichaelii</i> Debeaux, and <i>Aristolochia brasiliensis</i> Mart. & Zucc. Since 2017, the compound has been included in WADA's prohibited list, and its detection in the biological samples of professional athletes is regarded as a violation of the anti-doping rules. Cur

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21

Lo, Chi-Fang, and Chi-Ming Chen. "Acute Toxicity of Higenamine in Mice." Planta Medica 63, no. 01 (1997): 95–96. http://dx.doi.org/10.1055/s-2006-957619.

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22

Zaksaite, Salomeja. "Higenamine, Anti-Doping, and Plant-Based Cuisine: A Legal Analysis of Higenamine in Sport Anti-Doping Systems." Laws 11, no. 6 (2022): 82. http://dx.doi.org/10.3390/laws11060082.

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Current anti-doping policy seeks to protect honest athletes from biochemically overeducated colleagues. However, there is a question of whether the present policy has gone too far. This article illustrates the ambiguity of the anti-doping policy in the context of a particular plant-based substance (i.e., higenamine) by providing certain case studies. In such cases, the process of proof requires the continuous checking of suppositions since an athlete must establish how the prohibited substance could have entered his or her body. This obligation implies that an athlete and the defending team mu

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23

Zhang, Nana, Zeqin Lian, Xueying Peng, Zijian Li, and Haibo Zhu. "Applications of Higenamine in pharmacology and medicine." Journal of Ethnopharmacology 196 (January 2017): 242–52. http://dx.doi.org/10.1016/j.jep.2016.12.033.

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24

潘, 殿玲. "Determination of Higenamine in Weiyangning Pills by HPLC." Open Journal of Natural Science 09, no. 01 (2021): 48–53. http://dx.doi.org/10.12677/ojns.2021.91007.

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25

Li, Xiaojia. "Higenamine alleviates cerebral ischemia-reperfusion injury in rats." Frontiers in Bioscience 24, no. 5 (2019): 859–69. http://dx.doi.org/10.2741/4756.

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26

YU, FENGXIA, LINGTING KONG, and SHUJUAN WANG. "Influence of racemic higenamine on the sinus node." Experimental and Therapeutic Medicine 5, no. 2 (2012): 591–95. http://dx.doi.org/10.3892/etm.2012.813.

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27

Cohen, Pieter A., John C. Travis, Peter H. J. Keizers, Frederick E. Boyer, and Bastiaan J. Venhuis. "The stimulant higenamine in weight loss and sports supplements." Clinical Toxicology 57, no. 2 (2018): 125–30. http://dx.doi.org/10.1080/15563650.2018.1497171.

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28

Wong, Kai, Chi Lo, and Chi Chen. "Endothelium-Dependent Higenamine-Induced Aortic Relaxation in Isolated Rat Aorta." Planta Medica 63, no. 02 (1997): 130–32. http://dx.doi.org/10.1055/s-2006-957628.

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29

Tsukiyama, Muneo, Takuro Ueki, Yoichi Yasuda та ін. "β2-Adrenoceptor-Mediated Tracheal Relaxation Induced by Higenamine fromNandina domesticaThunberg". Planta Medica 75, № 13 (2009): 1393–99. http://dx.doi.org/10.1055/s-0029-1185743.

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30

Yu, Guo Rui, Bin Yao Wang, Dao Sheng Zheng, et al. "Effect of higenamine on action potential of ventricular myocardial cells." Journal of Electrocardiology 18, no. 1 (1985): 97–103. http://dx.doi.org/10.1016/s0022-0736(85)80040-6.

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31

Shin, Jung, Hye Yun-Choi, Eung Kim, and Myung Lee. "Inhibitory Effects of Higenamine on Dopamine Content in PC12 Cells." Planta Medica 65, no. 05 (1999): 452–55. http://dx.doi.org/10.1055/s-2006-960810.

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32

Wang, Yuhong, Jing Geng, Min Jiang, Cong Li, Yanxing Han, and Jiandong Jiang. "The cardiac electrophysiology effects of higenamine in guinea pig heart." Biomedicine & Pharmacotherapy 109 (January 2019): 2348–56. http://dx.doi.org/10.1016/j.biopha.2018.10.022.

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33

Zhang, Nana, Kai Qu, Minjie Wang та ін. "Identification of higenamine as a novel α1 -adrenergic receptor antagonist". Phytotherapy Research 33, № 3 (2019): 708–17. http://dx.doi.org/10.1002/ptr.6261.

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34

Boddupalli, S., D. Akrawi, and A. Hopkins. "Higenamine and Forskolin Induced Catecholaminergic Polymorphic Ventricular Tachycardia: A Case Report." Heart, Lung and Circulation 33 (August 2024): S429. http://dx.doi.org/10.1016/j.hlc.2024.06.657.

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35

Stajić, A., M. Anđelković, N. Dikić, et al. "Determination of higenamine in dietary supplements by UHPLC/MS/MS method." Journal of Pharmaceutical and Biomedical Analysis 146 (November 2017): 48–52. http://dx.doi.org/10.1016/j.jpba.2017.08.017.

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36

Park, Sun Oak, Chang Yee Hong, Seung Whan Paik, and Hye Sook Yun-Choi. "Determination of blood concentration of higenamine by high pressure liquid chromatography." Archives of Pharmacal Research 10, no. 1 (1987): 60–66. http://dx.doi.org/10.1007/bf02855622.

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37

Zhang, Yi, Jingjing Zhang, Chuntao Wu, et al. "Higenamine protects neuronal cells from oxygen‐glucose deprivation/reoxygenation‐induced injury." Journal of Cellular Biochemistry 120, no. 3 (2018): 3757–64. http://dx.doi.org/10.1002/jcb.27656.

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38

Kim, Nam Su, Chang Yee Hong, Chan Woong Pak, and Jung Kyoo Lim. "An Experimental Study on Adrenergic Effect of Higenamine in Rabbit Cardiovascular System." Korean Circulation Journal 16, no. 1 (1986): 1. http://dx.doi.org/10.4070/kcj.1986.16.1.1.

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39

Noh, Chung Il, Chang Yee Hong, Bong Ki Kim, Chan Woong Park, and Jung Kyoo Lim. "Chronotropic Actions of Higenamine in the Isolated Right Atrium of the Rabbit." Korean Circulation Journal 18, no. 2 (1988): 277. http://dx.doi.org/10.4070/kcj.1988.18.2.277.

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40

Qing, Ke, Hajime Terada, Shiho Sugiyama, et al. "Study on the mechanism for the positive inotropic effects of higenamine (II)." Journal of Cardiac Failure 4, no. 3 (1998): 102. http://dx.doi.org/10.1016/s1071-9164(98)90441-9.

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41

BAI, Gang, Yang YANG, Qian SHI, Ze LIU, Qi ZHANG, and Yuan-yuan ZHU. "Identification of higenamine in Radix AconitiLateralis Preparataas a beta2-adrenergic receptor agonist1." Acta Pharmacologica Sinica 29, no. 10 (2008): 1187–94. http://dx.doi.org/10.1111/j.1745-7254.2008.00859.x.

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42

Ueki, Takuro, Tatsuhiro Akaishi, Hidenobu Okumura, Tsuneo Morioka, and Kazuho Abe. "Biphasic Tracheal Relaxation Induced by Higenamine and Nantenine From Nandina domestica THUNBERG." Journal of Pharmacological Sciences 115, no. 2 (2011): 254–57. http://dx.doi.org/10.1254/jphs.10251sc.

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43

Ju, Ying, Chang-ming Wang, Juan-juan Yu, et al. "Higenamine inhibits acute and chronic inflammatory pain through modulation of TRPV4 channels." European Journal of Pharmacology 964 (February 2024): 176295. http://dx.doi.org/10.1016/j.ejphar.2023.176295.

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44

Yun-Choi, Hye Sook, Mi Kyung Pyo, Kyung Mi Park, Ki Churl Chang, and Duck Hyung Lee. "Antithrombotic Effects of YS-49 and YS-51—1-Naphthylmethyl Analogs of Higenamine." Thrombosis Research 104, no. 4 (2001): 249–55. http://dx.doi.org/10.1016/s0049-3848(01)00372-3.

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45

Ryu, Jae-Chun, Yun-Seon Song, Myungsoo Kim, Jung-Hyuck Cho, and Hye Sook Yun-Choi. "Identification of higenamine and its metabolites in rat by gas chromatography/mass spectrometry." Archives of Pharmacal Research 16, no. 3 (1993): 213–18. http://dx.doi.org/10.1007/bf02974485.

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46

Liu, Weixin, Yoshihiko Sato, Yoshisuke Hosoda, Koki Hirasawa, and Hiroyuki Hanai. "Effects of Higenamine on Regulation of Ion Transport in Guinea Pig Distal Colon." Japanese Journal of Pharmacology 84, no. 3 (2000): 244–51. http://dx.doi.org/10.1254/jjp.84.244.

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47

Muniz-Santos, Renan, Juliana Avezum, Bichara Abidão-Neto, and L. C. Cameron. "Dietary higenamine from Annonaceae family fruits as a possible source of unintentional doping." Forensic Science International 342 (January 2023): 111539. http://dx.doi.org/10.1016/j.forsciint.2022.111539.

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48

Kato, Eisuke, Shunsuke Kimura, and Jun Kawabata. "Ability of higenamine and related compounds to enhance glucose uptake in L6 cells." Bioorganic & Medicinal Chemistry 25, no. 24 (2017): 6412–16. http://dx.doi.org/10.1016/j.bmc.2017.10.011.

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49

Zhu, Jin-xing, Wang Ling, Chao Xue та ін. "Higenamine attenuates cardiac fibroblast abstract and fibrosis via inhibition of TGF-β1/Smad signaling". European Journal of Pharmacology 900 (червень 2021): 174013. http://dx.doi.org/10.1016/j.ejphar.2021.174013.

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50

Qiu, Jinping, Rui Zhang, Wentao Liu, Yang Lu, and Jifeng Liu. "Immunomagnetic bead-based sample preparation method and immunoassay for detecting higenamine in herbal products." Journal of Pharmaceutical and Biomedical Analysis 257 (May 2025): 116712. https://doi.org/10.1016/j.jpba.2025.116712.

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