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Dissertations / Theses on the topic 'Immunological investigation'

Author: Grafiati

Published: 4 June 2021

Last updated: 7 February 2022

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1

Souberbielle, Bernard. "Immunological investigation in multiple sclerosis." Thesis, University of St Andrews, 1993. http://hdl.handle.net/10023/13440.

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In this thesis, immunological parameters in multiple sclerosis patients (MS) have been examined and techniques contributing to the study of these aspects have been developed. The epidemiology of MS suggests that both environmental and genetic factors contribute to the disease process. According to a widespread theory, an autoimmune reaction leading to demyelination could be induced, in genetically determined individuals, by an infectious agent (s) e.g a paramyxovirus or a retrovirus. In the first part of the thesis, the oligoclonal immunoglobulin (Igs) in the CSF of MS patients in relation to the paramyxovirus SV5 were studied. This was to complement an initial observation made in the department that a significant proportion of cerebrospinal fluids (CSF) could have oligoclonal bands directly acting against antigens (Ags) of this virus. In the second part of the thesis, techniques were developed for the analysis of proliferative capacity of peripheral lymphocytes of MS and control patients using possible MS autoantigens viz myelin and brain vessels. The humoral response against these Ags was also assessed by immunoblotting using MS, neurological and normal control patients. In parallel and in the view to obtaining antigens for the immunological studies, techniques for the purification and characterisation of human brain vessels from post mortem brains were assessed and attempts to culture human brain endothelial cell lines were made.

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2

Budd, Timothy Charles. "An immunological investigation of neuromuscular transmission in insects." Thesis, University of Nottingham, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.334782.

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3

Boag, Alisdair Matthew. "An immunological and genetic investigation of canine hypoadrenocorticism (Addison's Disease)." Thesis, Royal Veterinary College (University of London), 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.618317.

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4

Sloper, K. S. "An immunological investigation into the aetiology of atopic eczema in children." Thesis, University of Oxford, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233526.

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5

O'Sullivan, Colette. "Epithelial mucins associated with breast carcinoma : purification, characterisation and immunological investigation." Thesis, University of Nottingham, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.290401.

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6

Oughterson, Susan Michelle. "Fish calcitonins and ultimobranchial gland - a histological, immunological and physiological investigation." Thesis, University of Salford, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.315453.

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7

Baker, Robert. "Investigation of glucocorticoid and local immunological function in tuberculosis and inflammation." Thesis, University College London (University of London), 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.249300.

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8

Cooper, Philip. "Onchocerciasis in Ecuador : a cellular immunological and epidemiological investigation of chorioretinopathy." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338137.

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9

Broadbent, Andrew James. "Chlamydia trachomatis : quantification, immunological investigation and co-infection with HIV-1." Thesis, Imperial College London, 2010. http://hdl.handle.net/10044/1/5929.

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Three aspects of Chlamydia trachomatis translational research were explored in this thesis. Firstly, as over 75% of patients with LGV are also HIV-1 sero-positive, a cell-culture model was established to investigate whether HIV-1 altered the replication of C. trachomatis serovar L2 in vitro. Inclusion size was significantly increased in the presence of HIV-1, but there was no significant alteration in chlamydial growth kinetics, infectivity, morphology, or transcription of 16sRNA, ompA or euo, suggesting that viral co-infection did not induce chlamydial persistence. It is, therefore, unlikely that the association of HIV-1 and LGV in vivo is due to an impact of HIV-1 on chlamydial replication in co-infected cells. Secondly, as there is no information on the chlamydial load shed by individuals with a rectal C. trachomatis infection, a qPCR assay was developed and used to determine the number of C. trachomatis organisms per rectal swab in NAAT-positive patients. The geometric mean chlamydial load was 5.0 x 105 organisms per swab (Standard Deviation, 152) and load was associated with proctitis, but not symptoms or HIV-1 infection. Asymptomatic individuals shed as much C. trachomatis as patients with rectal symptoms and might maintain transmission in the community. Finally, an ex vivo IFN-γ ELISpot assay was developed to characterise human cellular immune responses to the C. trachomatis-specific protein, Pgp3. T-cell epitopes were found along the length of the protein, but the magnitude of the immune responses was low. The Pgp3- induced IFN-γ response correlated with C. trachomatis exposure and was dynamic, decreasing after effective treatment. These observations suggest that Pgp3- induced IFN-γ may be useful as a biomarker for current infection, although the sensitivity and specificity of the ELISpot assay need improvement.

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10

Freedman, A. R. "Investigation of the immunological mechanisms involved in the pathogenesis of coeliac disease." Thesis, University of Cambridge, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.599204.

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11

Bruton, Rachel Kathyln. "An immunological and biochemical investigation of some central metabotropic glutamate receptors (mGluRs)." Thesis, University of Birmingham, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.340563.

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12

House, Arthur Kingsley. "An immunological and genetic investigation of anal furunculosis in German Shepherd dogs." Thesis, Royal Veterinary College (University of London), 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.479141.

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13

Michels, Chesney Elroy. "Investigation the immunological response elicited to the gastrointestinal nematode pinworm (Syphacia obvelata)." Doctoral thesis, University of Cape Town, 2006. http://hdl.handle.net/11427/9030.

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Includes bibliographical references.
It is important to emphasize with the advance of biotechnology and increased global exchange of animals and animal products, the risks of introducing adventitious infections. Previous studies of specitic-pathogen-free mouse colonies have identified the presence of infectious agents in 10-35% of research institutions investigated. Prevalence was higher among non-SPF mice with pinworm reported in 70% of institutions housing rodents under these conditions. Pinworm, a gastrointestinal (GI) nematode is commonly found in laboratory animals. The direct transmission of the parasite by contaminated food, water and bedding result in their continual re-exposure to the host, making the control of pinworm in animal holdings quite difficult. Syphacia obvelata, mouse pinworm, has been shown to interfere with research goals in several experimental models. In this study, we show the consequence of a pinworm outbreak in a transgenic barrier facility and define the immune response elicited in BALB/c mice. Infection with S. obvelata induced a transient Th2-type immune response with elevated cytokine production and parasite-specific IgG1. In contrast, HALB/c mice, deficient for IL-13, lL-4/13 or IL-4Ra showed chronic disease with more than 100-fold higher parasite burden, increased IFN-y production, parasite-specific IgG2b and a default Th2 response. Notably, infected lL-4-/- BALB/c mice showed only slight elevated parasite burden compared to controls, suggesting that IL-13 plays the dominant role in the control of S. obvelata. Furthermore, no significant eosinophilia, mastocytosis or goblet cell hyperplasia was induced. In a well-established ovalbumin (Ova) anaphylaxis model, we show that mice infected with S. obvelata induce a more severe anaphylactic reaction, with consistently greater temperature decline than their non-infected counterparts. Analysis of spleen cells further revealed a marked reduction of Ova-specific Th2 cytokines, highlighting the importance of pinworm free experimental mice. Finally, we generated anti-S. obvelata antibody to optimize the detection ELISA and identified target epitopes for future analysis. In conclusion, we identify the T helper immune response induced to S. obvelata and demonstrate the importance of IL-13 for the expulsion of the GI nematode. We show that S. obvelata induces a non-protective immune response to a common food allergen and confirm that the pinworm-specific ELISA is an effective diagnostic tool for detecting pinworm infected mice.

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14

Thilaganathan, Baskaran. "Investigation of fetal immunological development by flow cytometric analysis of circulating lymphocyte subpopulations." Thesis, King's College London (University of London), 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.244163.

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15

Morton, Angela Mary Young. "Investigation of T cell signalling events regulating immunity and tolerance in vivo." Thesis, Connect to e-thesis, 2008. http://theses.gla.ac.uk/59/.

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Thesis (Ph.D.) - University of Glasgow, 2007.
Ph.D. thesis submitted to the Division of Immunology, Infection and Inflammation, Faculty of Medicine, University of Glasgow, 2007. Includes bibliographical references.

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16

Theodorakopoulou, Eleni. "An investigation of late onset psoriasis." Thesis, University of Manchester, 2014. https://www.research.manchester.ac.uk/portal/en/theses/an-investigation-of-late-onset-psoriasis(eae40fae-4af4-4a30-ac0e-6f78281db60e).html.

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Psoriasis is a chronic, clinically heterogeneous, skin condition that affects approximately 2% of the general population. In 1985, Henseler and Christophers, classified psoriasis into early onset (EOP; age at onset ≤40 years-y) and late onset disease (LOP; age at onset >40 y). Previous research suggests that there are genetic and immunological differences between EOP and LOP. In particular, the major genetic determinant for psoriasis, the human leukocyte antigen (HLA)-Cw6 allele, occurs more frequently in EOP (55-80%) compared to LOP (15-20%) patients. Epidermal Langerhans’ cells (LC) migration is also different in these 2 subtypes of psoriasis. The primary aim of this thesis was to further explore the clinical, histological and immunohistochemical (IHC) differences between EOP and LOP. We compared clinical characteristics in a total of 497 subjects, including 340 psoriasis patients (108 recruited prospectively; 76 EOP and 32 LOP, mean age of onset 20.3±9.9 and 55.6±7y respectively, and 232 retrospectively; 202 EOP and 30 LOP, mean age of onset 20.7±9.9 and 55.2±7.2y respectively) and 157 controls (mean age 66±11.2y). Information on demographics, family history of psoriasis, clinical features, treatment and co-morbidities were recorded. Patients were also assessed for health-related quality of life and psychological distress. A total of 31 psoriasis patients, ≥ 50y of age, participated in the histological and IHC evaluation; 17 EOP and 14 LOP, mean age of onset 21.1±8.5 and 55.4±7.7y respectively. Skin biopsies were taken from involved (PP) and uninvolved (PN) skin and stained with haematoxylin and eosin (H&E) and IHC antibodies against various T-cell (CD3, CD4, and CD8) and LC (CD1α) markers. The H&E parameters (morphological and inflammatory) were graded with the use of a study specific histological score, whilst IHC positive epidermal cells were counted per microscopic field at 200X magnification. The dermal IHC infiltrate was assessed with a semi-quantitative (0-3) scale. Gender, body mass index, disease duration and severity, diagnosed hypertension and dyslipidemia were treated as covariates. The clinical data showed that LOP patients had a lower likelihood of having a positive family history of psoriasis (62% of EOP versus 35.6% of LOP patients; chi square-x2, P=0.001). In addition, patients with EOP parent(s) were 91% less likely to develop LOP than EOP (odds ratio-OR=0.093, P=0.025, 95% confidence interval-CI 0.012-0.74). Moreover, compared to LOP, EOP patients had a more severe disease (x2, P=0.021), usually requiring 3rd line treatments (x2; P=0.010). They also experienced frequent flares, following upper respiratory tract infections (x2, P=0.049). When data were segregated by age (≥50years) and after accounting for covariates, we observed that, compared to the non-psoriasis population, LOP patients were approximately 3 times more likely to develop type 2 Diabetes Mellitus (OR=2.56, P=0.05, 95% CI 1.01-6.54), whilst, EOP subjects were 98% less likely to develop autoimmune thyroiditis (OR=0.025, P=0.02, 95% CI 0.001-0.55). Psychologically, LOP patients were found to be a clinically more anxious group compared to EOP (t-test, P=0.006). Microscopically, the results from the H&E study showed an increased total inflammatory infiltrate in LOP, PP sections compared to EOP, PP ones (t-test, P=0.028). With IHC stains, we observed that in the epidermis of LOP PP, there was a significantly higher count of CD4+ cells; mean CD4+ in LOP of 15.1 ± 6.2 versus 6.7±4.6 in EOP (Analysis of variance-ANOVA, P<0.001). This subsequently led to a higher epidermal CD4+/CD8+ ratio of 1.3 in the LOP versus 0.5 for the EOP sections (ANOVA, P=0.002). In the PP dermis, CD4+ were also more abundant in the LOP tissue (x2, P=0.049). To assess whether these CD4+ cells were either T-lymphocytes or LC, we examined for differences in the CD3+ and CD1α+ cells. The mean epidermal CD3+ tended to be higher in LOP PP sections; mean epidermal CD3+ in the LOP 42.8 ± 13.3 versus 31.7 ± 17.5 in the EOP group (ANOVA, P= 0.061), while the dermal infiltrate showed a similar pattern (x2,P=0.067). Finally, there was no difference in epidermal and dermal CD1α+ and CD8+ cells in PP between EOP and LOP sections. These data indicate differences in clinical phenotype, heritability, comorbidities and immunopathomechanism between EOP and LOP. Taken together they provide further evidence that EOP and LOP may be different diseases.

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17

Beesley, Katrina M. "Expression of HBcAg fusion proteins in yeast and an investigation of their immunological properties." Thesis, Open University, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.292342.

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18

Remus, Natascha. "Genetic epidemiologic of the response to Mycobacterium tuberculosis : investigation of clinical and immunological phenotypes." Paris 5, 2008. http://www.theses.fr/2008PA05T042.

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La tuberculose (TB) demeure un problème majeur de la santé publique mondiale et il est connu que des facteurs génétiques influencent la susceptibilité de l'hôte à l'infection par M. Tuberculosis. Nous avons étudié les gènes NRAMP1, qui a été associé à la susceptibilité à la tuberculose dans plusieurs groupes ethniques, etIL12RBl, qui joue un rôle majeur dans la susceptibilité Mendélienne aux infections mycobactériennes (MSMD), et nous n'avons pas trouvé une association avec la TB pulmonaire dans une population adulte Marocaine. Par conséquent, la susceptibilité complexe à la TB maladie dans cette population semble être régie par d'autres mécanismes que la susceptibilité Mendélienne chez les enfants, ce qui pourrait expliquer les différentes présentations cliniques et mécanismes physiopathologiques dans ces différents groupes d'âge. Nous avons ensuite analysé les corrélations familiales de la réponse immunitaire à l'infection en étudiant la taille de l'intradermo réaction (IDR) à la tuberculine et la production d'IFNy de PBMC après stimulation avec des antigènes mycobactériennes dans in population d'une région d'incidence faible à modéré proche de Paris (Val de Marne). Nous avons observé une forte corrélation familiale pour la taille d'IDR, et après limitation de la population à des personnes non infectées, les corrélations de l'ESATô ont été à la limite significative (p = 0. 047). Ces études ont à la fois des implications scientifiques car ils améliorent la compréhension des mécanismes de contrôle immunitaire de l'infection, et des implications médicales, et permettent ainsi d'ouvrir la voie à de nouvelles stratégies de prévention et de thérapie
Tuberculosis (TB) remains a major global public health threat and host genetic factors are known to modulate the outcome of exposure to M. Tuberculosis. We studied the genes NRAMP1, which has been associated with TB susceptibility in several ethnic groups, and IL12RB1, that plays a major role in Mendelian Susceptibility to Mycobacterial Disease (MSMD) and found no evidence for association with pulmonary TB in an adult Moroccan population. Thus, complex susceptibility to TB disease in this population seems to be governed by other mechanisms than Mendelian susceptibility in children, which might explain the different clinical presentations and physiopathological pathways in these different age groups. We then analysed familial correlations of the immune response to infection studying the tuberculin skin test (TST) size and IFNy-production of PBMC's after stimulation with mycobacterial antigens in a population from a low to moderate incidence region near Paris (Val de Marne). We observed strong familial correlations for the TST, and after restriction of the study population to non-infected individuals, correlations for the ESAT6 trait were borderline significant (p=0. 047). These studies have both scientific implications, as they will enhance understanding of immune mechanisms controlling infection, and medical implications, as they will open the way for new preventive and therapeutic strategies

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19

Du, Plessis-Stoman Debbie. "An investigation of the in vitro anticancer properties of selected platinum compounds." Thesis, Nelson Mandela Metropolitan University, 2006. http://hdl.handle.net/10948/498.

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This dissertation mainly deals with some biochemical aspects regarding the efficacy of novel platinum anticancer compounds, as part of a broader study in which both chemistry and biochemistry are involved. Various novel diamine and N-S donor chelate compounds of platinum II and IV have been developed in which factors such as stereochemistry, ligand exchange rate and biocompatibility were considered as additional parameters. In the first order testing, each of these compounds was tested with reference to their “killing” potential by comparing their rate of killing, over a period of 48 hours with those of cisplatin and oxaliplatin. Some 80 compounds were tested in this way. Although only a few could be regarded as equal to or even better than cisplatin and oxaliplatin, the testing of these compounds on cancer cells provided useful knowledge for the further development of novel compounds. Four of the better compounds, namely Y9, Y14, Y16 and Lt16.2 were selected for further studies to obtain more detailed knowledge of their anticancer action, including some flow cytometric studies. In addition to the above, cisplatin resistant cells were produced for each of the three different cell lines tested, namely, HeLa, HT29 and MCF7 cancer cell lines, by intermittent and incremental exposure to cisplatin (all the cell lines tested became resistant to cisplatin). Each of the selected compounds were exposed to the cells in the same manner, in order to attempt the induction of resistance against these compounds in the three cell lines tested (i.e. whether these cells will become resistant to the various compounds). Each of these selected platinum containing compounds were subsequently tested against the “cisplatin resistant” cell lines in order to determine their efficacy against such cells. One such compound could be singled out, since cervical cancer cells (HeLa cells) do not become resistant to it. This behaviour is similar to that of oxaliplatin against cervical cancer and colon cancer (HT29) cells (oxaliplatin is the number one treatment for colon cancer at present). This compound also proved to be more active against cisplatin resistant cell lines. It was found that all the compounds induced apoptosis in the cell lines tested as well as inhibit the DNA cycle at one or more phase. Finally, an effort was made to evaluate the different compounds by comparing them with respect to their properties relating to anticancer action.

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20

Bell, Yvonne Louise. "An investigation into the induction of specific immunological unresponsiveness using donor major histompatibility complex antigens." Thesis, University of Oxford, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.386871.

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21

Massam, F. "A biochemical and immunological investigation of the extracellular polysaccharides produced from sugars by the oral streptococci." Thesis, Nottingham Trent University, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.332153.

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22

Masilamani, Madhan. "Immunological investigation of human complement receptor type II (CR2/CD21) : serum soluble CD21 in health and disease /." Konstanz, 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=967076668.

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23

Hauton, Christopher. "An investigation of potential immunological and metabolic indices of environmental water quality in the shore crab, Carcinus maenas." Thesis, University of Southampton, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.296142.

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24

Mercier, Helen Ceclie. "Investigation of the neutralizing activity for Treponema Pallidum of neonatal rabbit basal serum taken at 2, 3, and 4 weeks of age." CSUSB ScholarWorks, 1987. https://scholarworks.lib.csusb.edu/etd-project/397.

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25

Akbar, Moeed. "Investigation into the immunological effects of chromium and cobalt ions and wear debris released from metal-on-metal hip implants." Thesis, University of Strathclyde, 2011. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=16801.

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26

Moller, Marlo. "Human genetic susceptibility to tuberculosis : the investigation of candidate genes influencing interferon gamma levels and other candidate genes affecting immunological pathways." Thesis, Stellenbosch : University of Stellenbosch, 2007. http://hdl.handle.net/10019.1/1264.

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Thesis (PhD (Biomedical Sciences. Molecular Biology and Human Genetics))--University of Stellenbosch, 2007.
The infectious disease tuberculosis (TB) is one of the leading causes of death worldwide. The idea that infectious diseases are the most important driving force in natural selection and that they sustain frequent polymorphisms in the human genome was formally suggested by Haldane in 1949. This hypothesis implicated the human genetic component in the response to infectious disease. Today the involvement of host genetics in TB has been proven unequivocally and, together with environmental factors (e.g. nutrition and crowding) and the causative bacterium, Mycobacterium tuberculosis (M.tuberculosis), may influence the outcome of disease. As is evident, TB is a complex disease and the implication for studying genetic susceptibility is that a number of genes will be involved. Interferon gamma (IFN-7) is the major macrophage-activating cytokine during infection with M.tuberculosis and its role has been well established in animal models and in humans. This cytokine is produced by activated T helper 1 (Th1) cells. These Th1 responses can best deal with intracellular pathogens such as M.tuberculosis. We selected twelve candidate genes based on the hypothesis that genes which regulate the production of IFN-7 may influence TB susceptibility. We also selected polymorphisms from 27 other candidate genes, which may affect immunological pathways involved in TB, to investigate as susceptibility factors based on the following hypotheses: 1) granulomatous diseases can share susceptibility genes; 2) gene expression studies done by DNA-array analysis experiments may reveal TB susceptibility genes; 3) genomewide linkage studies in TB can determine susceptibility loci and genes in this region are possibly susceptibility factors; and 4) functional susceptibility polymorphisms in genes involved in immune-mediated diseases other than TB may contribute to susceptibility to TB. This research tested the association of 136 genetic polymorphisms in 39 potentially important genes with TB in the South African Coloured population. Well-designed case-control association studies were used and we attempted to replicate these findings in an independent sample set using family-based case-control designs (transmission disequilibrium tests (TDTs)). In addition, haplotypes and linkage disequilibrium (LD) in the candidate genes were also investigated. During the case-control analyses we found significant associations for 6 single nucleotide polymorphisms (SNPs) in the following genes: SH2 domain protein 1A, tolllike receptor 2, class II major histocompatibility complex transactivator, interleukin 1 receptor antagonist, runt-related transcription factor 1 and tumour necrosis factor superfamily, member 1B. Discrepant results were obtained during the TDT analyses. The number of families available was small and for this reason we cannot conclude that the case-control results were spurious. We also tested the association of haplotypes with TB. Haplotypes in the interleukin 12, beta (IL12B) and toll-like receptor 4 genes were nominally associated with TB in both the case-control and TDT analyses. We observed strong LD for the genes in the South African Coloured population. In total 17 novel SNPs were identified and one novel allele was found for a microsatellite in IL12B. This research contributes to the increasing amount of information available on genes involved in TB susceptibility, which in the future may help to predict high risk individuals.

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Alkhatib, Shaza. "Investigation of the immunological differences between granulocytes from healthy donors and breast cancer patients, with respect to the cancer testis antigen, CTCFL." Thesis, University of Essex, 2015. http://repository.essex.ac.uk/16454/.

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Granulocytes or Polymorphonuclear neutrophils (PMNs) are key players in the non-specific immune system against microbial infection as they express surface receptors for the recognition of general antigenic patterns found on pathogens. In addition PMN’s also act as a mediator for the antigen-specific adaptive immunity and T-cells function. However, an anti-tumoral role of the PMN’s was suggested over the recent years beside their well-studied innate function, opening the door for new studies in this area. CTCFL or BORIS (Brother Of the Regulator of the Imprinting Site) is a paralogue protein to a ubiquitously expressed transcription factor, CTCF. The expression of CTCFL was detected exclusively in the spermatocytes. The role of BORIS was then supposed to be linked to the de novo DNA-methylation and re-establishment of methylation markers, however, a full understanding its function calls for further investigations. CTCFL was also recently established as a member of the cancer-testis (CT) gene family as its activation in somatic cells was found to be associated with the development of many types of cancer, including breast cancer. This presents it as an attractive biomarker for cancers dia gnosis/prognosis and therapy target. Interestingly, CTCFL was also detected in PMNs obtained from breast cancer patients while being undetectable in the PMN’s of healthy donors. Again this supports the prospect of using this protein as a biomarker in the early detection of cancer and treatment. In this project we aimed to investigate the effect of CTCFL expression in PMNs of breast cancer patients on the physiological characteristic and immunological functions of these leukocytes. The first part of our experiments assessed phagocytic activity, oxidative burst function, survival and expression of immunity receptors in BORIS-positive PMN cell-line model. The second part investigated the origin of CTCFL activation by the incubation of peripheral human PMN’s with the serum of breast cancer patients or the adenocarcinoma cell lines and observe the possible alteration in CTCFL levels and the corresponding levels of the immune system receptors. Finally, we attempted to manipulate the levels of CTCFL in PMN cell line model (knock down or over-expression) and the effect it may produce on the expression of immune system receptors.

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28

Netter, Petra [Verfasser], and Tobias [Akademischer Betreuer] Dick. "Investigation of Natural Killer cell detachment from tumor cells - Characterizing the termination of the activating Natural Killer cell immunological synapse / Petra Netter ; Betreuer: Tobias Dick." Heidelberg : Universitätsbibliothek Heidelberg, 2014. http://d-nb.info/1179924487/34.

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Potthast, Maria [Verfasser], Carsten [Akademischer Betreuer] Schmidt-Weber, Dirk [Gutachter] Haller, and Carsten [Gutachter] Schmidt-Weber. "Investigation into the role of the transcription factor RelB for the immunological tolerance induction / Maria Potthast ; Gutachter: Dirk Haller, Carsten Schmidt-Weber ; Betreuer: Carsten Schmidt-Weber." München : Universitätsbibliothek der TU München, 2021. http://d-nb.info/1236343174/34.

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30

Brown, Jennifer L. "A molecular and immunological investigation of cellular responses to dengue virus identification of potentially upregulated host genes and the constructionof a vaccinia virus expressing the dengue 1 Hawaii NS3 protein." Link to electronic version, 2000. http://www.wpi.edu/Pubs/ETD/Available/etd-0330100-124248/.

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31

Scholey, Andrew Belton. "Immunological investigations into synaptic plasticity." Thesis, Open University, 1991. http://oro.open.ac.uk/57339/.

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Antibody technology was applied to the study of synaptic plasticity resulting from passive avoidance training in the chick and long-term potentiation in the rat. These studies fell into three categories: 1) the disruption of memory for a one-trial passive avoidance task by intracranial injection of an anti-postsynaptic density antiserum 2) mapping time- and locus-specific changes in the chick brain using antisera to the cytoskeletal proteins (Xtubulin and microtubule-associated protein 2 following passive avoidance training and 3) using antibodies to synaptically-enriched antigens to map time- and locus-specific changes in hippocampal subfields following long-term potentiation in the rat. In the first series of experiments an antiserum (RI4) was raised against protein from chick forebrain postsynaptic densities (PSDs). The antiserum was characterised and was found to recognise six distinct antigens as determined by Western Blots. These antigens were found to have a primarily (but not exclusively) synaptic location. Intracranial injections of IgG isolated from R14 resulted in amnesia for a one-trial passive avoidance paradigm in the chick when administered 60min pre-training (but not 30min or 15min pre-training or 1000n post-training), in chicks tested 24hrs (but not Ihr or 3hrs) post-training. In the second set of experiments monoclonal antibodies were used to examine changes in levels of the cytoskeletal proteins a-tubulin and microtubule-associated protein 2 (MAP2) in specific forebrain loci following passive avoidance training in the chick. Of the regions examined, elevations in the titre of anti-a-tubulin were found in the left Intermediate Hyperstriatum Ventrale (IMHV) l hr, 6hrs and 24hrs following passive training, in the left Lobus Parolfactorius (LPO) 1hr following training and in the right LPO 6hrs and 24hrs following training. A hemispherically-asymmetrical change was found in the titre of anti-MAP2 which was interpreted as possibly reflecting a decrease in the amount of the antigen in the left IMHV 24hrs following training. No training-related changes were detected, using either antibody, in a third forebrain region, the Paleostriatum Augmentatum (PA). During the characterisation of antiserum R14 it was found that only one antigen (with an apparent molecular weight of 230kDa) is conserved between the chick and rat brain. The antigen is enriched in synaptic fractions isolated from the rat hippocampus and was used, as well as a PSD-specific monoclonal antibody, 411B, to examine possible changes in hippocampal subfields CAl, CA3 and the dentate area taken at several time-points following tetanisation of the right perforant path. 24hrs following tetanisation (but not at earlier time-points), the titre of R14 was elevated in the dentate area ipsilateral to tetanisation and in both the ipsi- and contralateral CAL The titre of 411B was increased specifically in the target, dentate area and only at 8hrs following tetanisation, an increase which was abolished in the presence of the protein synthesis inhibitor, anisomycin. These results are discussed in the context of current models of synaptic plasticity.

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Brown, Jennifer L. "A Molecular and Immunological Investigation of Cellular Responses to Dengue Virus: Identification of Potentially Upregulated Host Genes and the Construction of a Vaccinia Virus Expressing the Dengue 1 Hawaii NS3 Protein." Digital WPI, 2000. https://digitalcommons.wpi.edu/etd-theses/187.

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The purpose of this thesis for the degree of Master of Science was to use molecular and immunological techniques to study cellular responses to dengue virus infection. In the initial study, Differential Display was used to compare mRNA expression in dengue-infected K562 cells and mock-infected cells. Cloning and sequencing were then used to identify cellular genes that were potentially up-regulated in response to Dengue virus infection. These genes included bleomycin hydrolase and a dystrophin homologue. The goal of the later part of this research was to construct a recombinant vaccinia virus expressing the dengue 1 Hawaii NS3 protein. Cytotoxic T-lymphocyte assays and protein gel electrophoresis showed that the NS3 protein was being expressed. This construct was then used to study the cytotoxic T-cell response of a dengue 1 vaccine recipient. The results of this study showed that this individual has dengue 1 NS3 specific T-cells and also that this vaccinia virus can be used for subsequent T-cell studies.

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33

Mitchell, B. S. "Immunological investigations of the rat metrial gland." Thesis, University of Southampton, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373978.

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Chevrier, Véronique. "Identification de composants centrosomaux à l'aide d'anticorps monoclonaux." Grenoble 1, 1992. http://www.theses.fr/1992GRE10196.

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L'initiation, la croissance et l'etablissement de la polarite des microtubes s'effectuent a partir de sites specialises appeles centres organisateurs des microtubules. Dans les cellules animales, le principal mtoc est le centrosome. Dans le but d'identifier des composants centrosomaux, des anticorps monoclonaux diriges contre des centrosomes isoles a partir de thymocytes de veau ont ete produits. Deux anticorps monoclonaux ont ete particulierement etudies. L'anticorps monoclonal 6c6 reconnait une proteine de 180 kda, localisee au niveau du materiel pericentriolaire dans differentes lignees cellulaires animales. Au cours du cycle cellulaire, un marquage transitoire apparait au niveau des kinetochores et du corps intermediaire, suggerant des connexions entre ces diverses organelles mitotiques. Dans les cellules de plantes superieures, cet anticorps presente un marquage specifique des centres organisateurs potentiels des microtubules. L'epitope reconnu par l'anticorps 6c6 semble donc etre un constituant commun de mtocs des cellules animales et vegetales. L'anticorps monoclonal 2e10 a permis la mise en evidence d'une proteine de 280 kda, associee non seulement aux centrosomes mais aussi aux plaques d'adhesion. Cette proteine a ete appelee centroplaquine. Reciproquement, nous avons montre que la vinculine, un constituant connu des plaques d'adhesion, est aussi localisee dans le centrosome. Ces proteines pourraient appartenir a un complexe permettant de connecter le reseau d'actine aux centrosomes et a la membrane plasmique. En conclusion, toutes ces etudes montrent qu'il existe des correlations fonctionnelles entre le centrosome et diverses structures cellulaires

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35

Amin, Tehmina. "A novel immunological probe for investigating enterotoxin biogenesis." Thesis, University of Kent, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.296722.

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36

Hüber, Christian Markus. "Investigating adaptive immunological features of natural killer cells." Thesis, University of Cambridge, 2015. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708920.

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37

Thomas, Paul David. "Clinical and immunological investigations on the pathogenesis of pouchitis." Thesis, King's College London (University of London), 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.391128.

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38

Botes, Annelise. "Immunological and epidemiological investigations in South African ostriches and penguins." Thesis, Stellenbosch : Stellenbosch University, 2004. http://hdl.handle.net/10019.1/53747.

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Dissertation (PhD)--University of Stellenbosch, 2004
ENGLISH ABSTRACT: Newcastle disease (NO) and mycoplasma infections in ostriches have considerable economic implications for the South African ostrich industry in that NO is a limiting factor in the export of ostrich products to the European Union and mycoplasma infections cause stock losses, reduced production, reduced hatchability and downgrading of carcasses. In the first section of this dissertation, the role of passively acquired and mucosal immunity in protection of ostrich chicks against Newcastle disease virus (NOV) was investigated. Ostrich hen serum IgG and yolk IgY were isolated and characterized, and the transfer of maternal anti-NOV antibodies to the egg yolk was determined using an enzyme-linked immunosorbent assay (ELISA). Results indicated that anti-NOV antibodies were successfully transferred from the ostrich hen to the egg yolk. In addition, ostrich IgA was isolated, characterized and rabbit anti-ostrich IgA antibodies produced and used for measuring mucosal anti- NOV IgA antibodies produced in response to mucosal vaccination. Results indicated that the live La Sota vaccine stimulates IgA production and thus mucosal immunity in ostrich chicks. In the second section of this dissertation, ostrich mycoplasmas were isolated and identified using 16S rRNA gene sequencing. These sequences indicated that ostriches carry three unique mycoplasmas, which are phylogenetically quite divergent. The 16S rRNA gene sequences of the ostrich mycoplasmas were subsequently used for the development of specific primers for the detection and diagnosis of mycoplasma infections in ostriches by PCR. The last section of this dissertation focuses on avian malaria in African penguins and the management of this disease during rehabilitation. The Foundation for the Conservation of Coastal Birds (SANCCOB) is a seabird rescue and rehabilitation centre, which is largely dedicated to the rehabilitation of diseased, injured and oiled penguins. Significant mortalities due to avian malaria occur at this facility. The aim of this study was the development of an ELISA for the purpose of assessing the natural levels of anti-Plasmodium antibodies in African penguins on entry into the SANCCOB facility and during rehabilitation. Results indicated significant increases in anti- Plasmodium antibody levels after entry, which was not influenced by oiling. Infection with malaria and not parasite recrudescence was viewed to be the cause of this increase, indicating a possible role of the SANCCOB facility in exposing penguins to avian malaria.
AFRIKAANSE OPSOMMING: Newcastlesiekte (NS) en mikoplasmainfeksies in voltruise het geweldige ekonomiese implikasies vir die Suid-Afrikaanse volstruisbedryf. Die rede hiervoor is dat NS 'n beperkende faktor in die uitvoer van volstruisprodukte na die Europese Unie is, en mikoplasmainfeksies tot kudde verliese, verlaagde produksie en uitbroei asook lae gradering van karkasse lei. In die eerste gedeelte van hierdie proefskrif is die rol van passiewe- en mukosale-immuniteit in die beskerming van volstruiskuikens teen NS virus (NSV) ondersoek. Volstruishenserum IgG en eier IgY is geïsoleer en gekarakteriseer en die oordrag van maternale anti-NSV antiliggame na die eier ondersoek met behulp van 'n 'enzyme-linked immunosorbent assay' (ELISA). Resultate het getoon dat anti-NSV antiliggame suksesvol van die hen na die eier oorgedra is. Volstruis IgA is ook geïsoleer, gekarateriseer en konyn anti-volstruis IgA antiliggame geproduseer wat gebruik is vir die bepaling van mukosale anti-NSV IgA antiliggame in reaksie op mukosale immunisering. Resultate het getoon dat lewendige La Sota entstof IgA produksie stimuleer en dus tot mukosale-immuniteit in volstruiskuikens lei. In die tweede gedeelte van hierdie proefskrif is volstruismikoplasmas geïsoleer en geïdentifiseer met behulp van 16S rRNA geenopeenvolgingsbepalings. Hierdie volgordes het getoon dat drie unieke mikoplasmas in volstruise voorkom wat filogeneties verskillend blyk te wees. Die 16S rRNA geenopeenvolgings van die volstruismikoplasmas is gebruik vir die ontwikkeling van spesifieke inleiers vir die PKR identifisering en diagnose van mikoplasmainfeksies in volstruise. Die laaste gedeelte van hierdie proefskrif fokus op voëlmalaria in die Afrika pikkewyn en die bestuur van hierdie siekte gedurende rehabilitasie. Die 'South African Foundation for the Conservation of Coastal Birds' (SANCCOB) is 'n seevoëlreddingsen rehabilitasie-sentrum vir siek, beseerde en ge-oliede pikkewyne. Hierdie sentrum het egter aansienlike vrektes as gevolg van voëlmalaria. In hierdie studie is 'n ELISA ontwikkel vir die bepaling van natuurlike anti-Plasmodium antiliggaamvlakke van pikkewyne by aankoms en tydens rehabilitasie by SANCCOB. Resultate het 'n toename in anti-Plasmodium antiliggaamvlakke getoon na toelating wat nie beïnvloed is deur olie nie. Hierdie toename kan toegeskryf word aan nuwe malariainfeksies en nie 'n heruitbraak van bestaande infeksies nie wat daarop dui dat pikkewyne aan voëlmalaria blootgestel word by die SANCCOB-sentrum.

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39

Konrad, Josef. "In vitro culture of Babesia divergens : biochemical and immunological investigations." Thesis, Imperial College London, 1986. http://hdl.handle.net/10044/1/38073.

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40

Sai, Pierre. "Etude de l'auto-immunite dirigee contre les cellules pancreatiques a insuline au cours des diabetes insulinoprives." Nantes, 1987. http://www.theses.fr/1987NANT2062.

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Pathogenie autoimmune des diabetes insulinoprives chez l'homme, la souris et le rat. Des lymphocytes circulants alterent in vitro l'insulinosecretion des cellules d'ilots et peuvent presenter des anomalies du circuit de l'interleukine 2. Essai d'immunotherapie par la ciclosporine a

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41

au, joyceand@iinet net, and Jocye A. Eade. "Investigating porcine and feline zona pellucida as immunocontraceptive antigens in the female domestic cat." Murdoch University, 2007. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20070717.124229.

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Immunocontraception, or contraception mediated by the immune system, is being widely studied as an alternative, humane form of population control. The induction of an immune response against a specific component of the mammalian oocyte, termed zona pellucida (ZP) has been shown to be an effective immunocontraceptive in several species. Comparatively little work has been done investigating the use of ZP antigens in the domestic cat. This study aimed to investigate porcine ZP(B+C) and feline ZPA, B and C as immunogens in the domestic cat, and further to elucidate their effects on reproduction. Immunisation of female cats with porcine ZP(B+C) failed to elicit a detectable antibody response as assessed by ELISA, immunoblotting and immunohistochemistry. Additionally, there was no effect on the structure of the ovaries nor on breeding performance. Feline ZPA, B and C were cloned into the pkCMVint.polyli mammalian expression vector and used to immunise female cats. ELISA revealed that immunisation with either pkCMVint.fZP(B+C) or pkCMVint.fZPA DNA (Treated) resulted in a low-level circulating antibody response, which was apparently short-lived. Immunoblotting did not reveal any common pattern of recognition of antigenic polypeptides between responding animals. Ovaries from fZP Treated animals, however, showed antibody binding specifically on the ZP of follicles from late primary / early secondary, through to antral stages. Despite the antibody binding, the overall structure of the ovaries remained unaffected in all but two of the fZP Treated cats. Two fZPA immunised cats exhibited ovaries that had no recognisable follicular structures, however, the observed abnormalities could not be conclusively linked to fZPA immunisation. Overall, a significant lymphoproliferative response was shown in fZP Treated cats when compared with Controls. Fertility was not significantly affected in fZP Treated cats, although there was a tendency towards increased incidence of unsuccessful matings or pregnancies. The fZP DNA preparations studied here exhibit potential as an immunocontraceptive, with the ability to generate a lymphoproliferative response against fZP and elicit antibodies specifically recognizing fZP in situ. Further studies should continue to investigate the immunogenicity of, and characterise the immune response against, such fZP DNA preparations.

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42

Ball, Andrew. "Immunological investigations into the properties and function of the mammalian phosphatidylinositol transfer protein." Thesis, University College London (University of London), 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.264705.

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43

Eade, Joyce A. "Investigating porcine and feline zona pellucida as immunocontraceptive antigens in the female domestic cat /." Access via Murdoch University Digital Theses Project, 2007. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20070717.124229.

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44

Schoelzel, K. "Investigating immunological and metabolic pathways involved in the pathogenesis of chronic liver disease." Thesis, University College London (University of London), 2017. http://discovery.ucl.ac.uk/1553193/.

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Chronic liver disease affects a large number of people with a rising incidence, and is characterized by the development of liver fibrosis, eventually resulting in cirrhosis, Treatment options for end-stage liver disease are limited and liver cirrhosis represents the main risk factor for hepatocellular carcinoma (HCC), highlighting the need for novel therapeutic approaches. In this thesis, metabolic as well as immunological pathways contributing to the development of liver fibrosis and subsequent HCC have been investigated. Hepatic stellate cells (HSCs) are considered as the key players in fibrosis development, as their activation results in excessive extracellular matrix deposition, leading to the establishment of liver fibrosis. In addition, tumour-stromal interactions between HSCs and HCC play a role in HCC pathogenesis, and are therefore considered as a potential target for novel HCC therapy. In this thesis, the role of the AMPK pathway in tumour-stromal interactions between HSCs and HCC has been investigated, with a special focus on pharmacologically targeting AMPK in both HSCs and HCC. Moreover, the development of liver fibrosis and cirrhosis is accompanied by a chronic inflammatory immune response, fostering a pro-fibrogenic environment, especially in the context of autoimmune liver disease (AILD). Mucosal-associated invariant T cells (MAIT cells) are a recently discovered subset of innate like T cells that represent up to 40% of all liver lymphocytes. Besides being crucial for anti-microbial defence, MAIT cells contribute to autoimmune diseases and are thought to play an important role in tissue inflammation in the liver, as they secrete pro-inflammatory cytokines upon activation. Therefore, the role of MAIT cells for the development of liver fibrosis in AILD was explored. More specifically, the MAIT cell subset was characterized in human patients with AILD, and mechanisms of MAIT cell activation, as well as MAIT cell - HSC interactions were investigated.

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45

Dor, Frank Johan Marinus Frederik. "Investigations relating to the induction of immunological tolerance through spleen transplantation in miniature swine." [S.l.] : Rotterdam : [The Author] ; Erasmus University [Host], 2006. http://hdl.handle.net/1765/10508.

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46

Ongagna, Jean-Claude. "Histoire naturelle du diabète insulino - dépendant de l'enfant conséquences dans la prédiction." Paris 5, 1996. http://www.theses.fr/1996PA05S019.

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Le diabète insulino-dépendant (DID) est une maladie auto-immune occasionnée par la destruction des cellules bêta insulaires. Les signes cliniques caractéristiques traduisant l'élévation permanent des signes glycémiques sont précédés d'une phase asymptomatique au cours de laquelle on peut reconnaitre des stigmates d'auto-immunité contre le pancréas endocrine au premier rang desquels les ICA. Cette phase pré-diabétique a été essentiellement étudiée chez frères et sœurs de DID. La présente étude a cherché à évaluer la fréquence des autoanticorps anti-ilots pancréatiques et leur âge d'acquisition chez les enfants très jeunes (moins de 7 ans), et nés d'au moins un parent diabétique insulino-dépendant. Nos résultats montrent que la fréquence des autoanticorps anti-ilots est très élevée chez ces enfants, avec autant D'ICA que d'autres autoanticorps. Ce travail montre également que les marqueurs de l'immunité humorale anti-ilots apparaissent très tôt dans la vie de ces enfants. Les enfants nés de parents DID développent les autoanticorps in utero. Cette étude ne révèle aucune distorsion dans la fréquence des différents autoanticorps en fonction du sexe du parent diabétique. Les résultats préliminaires du suivi des enfants inclus montrent un taux de séroconversion important (2,7%) après un an.

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Brunet, Jean-François. "Recherche et caractérisation des activités anticorps des liquides kystiques des tumeurs cérébrales." Université Joseph Fourier (Grenoble ; 1971-2015), 1994. http://www.theses.fr/1994GRE10222.

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Le but de cette these est d'etudier les reponses immunes humorales intracerebrales lors de processus tumoraux cerebraux. Elle comprend donc une introduction sur les interactions entre les systemes immunitaire et nerveux central d'une part et sur les tumeurs cerebrales d'autre part. Puis une presentation du materiel biologique donne notamment les raisons du choix des liquides kystiques pour cette etude immunologique. Le troisieme chapitre decrit les etudes qualitative et quantitative des immunoglobulines des liquides kystiques des tumeurs cerebrales. Les deux chapitres suivants presentent la recherche des activites anticorps des liquides kystiques dirigees respectivement contre le tissu cerebral non tumoral et contre les tissus cerebraux tumoraux, par immunohistochimie et par immunotransfert. Les effets des immunoglobines de classe g des liquides kystiques, sur des lignees cellulaires, sont decrits dans le sixieme chapitre. Puis les protocoles developpes pour la purification des antigenes reconnus par les liquides kystiques sont exposes, avant une discussion generale sur l'ensemble des resultats

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48

Thiart, Hanlie. "Immunological and epidemiological investigations into avian malaria in the African penguin during rehabilitation and in breeding colonies." Thesis, Stellenbosch : University of Stellenbosch, 2005. http://hdl.handle.net/10019.1/16620.

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Thesis (MSc)--University of Stellenbosch, 2005.
ENGLISH ABSTRACT: The African penguin, which occurs along the south-eastern and south-western shores of South-Africa and Namibia, has experienced a severe reduction in population numbers due to guano and egg collection in the first half of the 19th century, and oil pollution in the second half of the 19th century as a result of oil tankers rounding the Cape of Good Hope. The population would have been reduced by a further 19% had it not been for the rehabilitation of penguins at the South African National Council for the Conservation of Coastal Birds (SANCCOB) facility. Although this has been very successful, mortalities as a result of avian malaria infection have considerably reduced the efficiency of rehabilitation. In an effort to assess the role of immunity against malaria in combating the disease, an enzyme-linked immunosorbent assay (ELISA) for the detection of antibody levels to avian malaria was developed. The ELISA was used to detect antibody levels to avian malaria of penguins on entry and during rehabilitation from October 2001 to January 2003. The aim of this study was to continue the determination of antibody levels to avian malaria of penguins entering the SANCCOB facility, in order to allow an evaluation of the antibody levels to avian malaria for two full calendar years. This investigation was combined with a polymerase chain reaction (PCR)-based method, capable of detecting any Plasmodium species in penguin serum. These two methods were also used to investigate avian malaria in several breeding colonies in order to assess the role avian malaria may play in the survival of the African penguin in the wild. Results indicated that the ability of penguins to produce anti-Plasmodium antibodies was not influenced by oiling and that infection with malaria was not due to recrudescence but rather due to infection via mosquitoes. This indicated a possible role of the SANCCOB facility in exposing the penguins to avian malaria. However a large number of penguins arrived at the facility previously infected with malaria, indicating that malaria was present in the breeding colonies. Investigations in the breeding colonies revealed extremely high avian malaria prevalence even though no sick birds or mortalities were observed. This raised the question whether different types of malaria are responsible for infection in the SANCCOB facility and breeding colonies.
AFRIKAANSE OPSOMMING: Die Afrika Pikkewyn kom langs die suid-oostelike en suid-westelike kus van Suid Afrika en Namibië voor. In die afgelope eeu het hierdie spesie ‘n geweldige afname in populasie getalle ondervind. Dit was hoofsaaklik die gevolg van die versameling van guano en pikkewyneiers in die eerste helfte van die 19de eeu en oliebesoedeling in die tweede helfde van die 19de eeu. Die “South African Foundation for Conservation of Coastal Birds” (SANCCOB) is ‘n seevoëlreddings- en rehabilitasiesentrum vir siek, beseerde en ge-oliede pikkewyne. Dit word geskat dat die Afrika Pikkewyn populasie met ‘n verdere 19% sou afgeneem het as dit nie vir die rehabilitasie by die SANCCOB sentrum was nie. Hierdie sentrum het egter aansienlike vrektes in die somer as gevolg van voëlmalaria, wat sodoende die effektiwiteit van die rehabilitasie verlaag. In ‘n poging om die rol van immuniteit teen malaria te bepaal is ‘n “enzyme-linked immunosorbent assay” (ELISA) ontwikkel vir die bepaling van antiliggaam vlakke teen malaria. Hierdie ELISA is gebruik vir die bepaling van die anti-Plasmodium antiliggaam vlakke van die pikkewyne by aankoms en ten tye van rehabilitasie by SANCCOB vanaf Oktober 2001 to Januarie 2003. Die doel van hierdie studie was eerstens om hierdie ELISA bepalings voort te sit om sodoende antiliggaam vlakke teen malaria oor twee kalender jare te kan evalueer. Hierdie ondersoek was gekombineer met ‘n polimerase ketting reaksie (PCR) metode, wat enige Plasmodium spesie in pikkewynserum sou kon opspoor. Hierdie twee metodes is ook gebruik vir ondersoeke in sommige broeikolonies, met die doel om te bepaal watter rol voëlmalaria in die oorlewing van die Afrika pikkewyn in die natuur speel. Resultate het getoon dat olie nie die vermoë van die pikkewyn beïnvloed om anti- Plasmodium antiliggame te vervaardig nie en dat malaria infeksie hoofsaaklik deur muskiete veroosaak word en nie deur heruitbraak van ‘n bestaande infeksie nie. Dit dui egter daarop dat pikkewyne blootgestel word aan voëlmalaria by die SANCCOB sentrum. Daar is ook gevind dat ‘n groot aantal pikkewyne met malaria infeksies by die sentrum opgedaag het wat dui op die voorkoms van malaria in die broeikolonies. Ondersoeke in die broeikolonies het ‘n besonder hoë voorkoms van malaria onthul. Geen vrektes of siek pikkewyne is in die broeikolonies waargeneem nie, wat moontlik kan beteken dat pikkewyne by SANCCOB met ‘n ander tipe malaria geïnfekteer word as in die broeikolonies.

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49

Quigley, R. L. "Investigation of the mechanism of induction of immunologic unresponsiveness to renal allografts by blood transfusion." Thesis, University of Oxford, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233514.

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50

Huff, Courtney L. "Investigating the binding of streptococcal monoclonal antibody 10F5 in the heart of the Lewis rat." CardinalScholar 1.0, 2009. http://liblink.bsu.edu/uhtbin/catkey/1538086.

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