Relevant bibliographies by topics / Inositolphosphates

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'Inositolphosphates'

Author: Grafiati
Published: 16 February 2023

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Inositolphosphates.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Inositolphosphates"

1

Etoh, S., M. Ohashi, A. Baba, and H. Iwata. "Ibudilast inhibits inositolphosphates formation in guinea pig lung." European Journal of Pharmacology 183, no. 3 (July 1990): 761–62. http://dx.doi.org/10.1016/0014-2999(90)92565-z.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Kvasnička, František, Jana Čopíková, Rudolf Ševčík, Eliška Václavíková, Andriy Synytsya, Kateřina Vaculová, and Michal Voldřich. "Determination of phytic acid and inositolphosphates in barley." ELECTROPHORESIS 32, no. 9 (March 31, 2011): 1090–93. http://dx.doi.org/10.1002/elps.201000578.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Tian, Yuemin, Rainer Schreiber, Podchanart Wanitchakool, Patthara Kongsuphol, Marisa Sousa, Inna Uliyakina, Marta Palma, et al. "Control of TMEM16A by INO-4995 and other inositolphosphates." British Journal of Pharmacology 168, no. 1 (December 18, 2012): 253–65. http://dx.doi.org/10.1111/j.1476-5381.2012.02193.x.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Whelan, J. P., W. T. Shearer, E. B. Gilliam, and K. J. Hardy. "A protein kinase C-activating phorbol ester accelerates the T cell antigen receptor-stimulated phosphatidylinositol cycle in normal human CD4+ T cells." Journal of Immunology 148, no. 9 (May 1, 1992): 2872–78. http://dx.doi.org/10.4049/jimmunol.148.9.2872.

Full text
Abstract:
Abstract Ligation of the TCR on Jurkat T lymphoblastoid cells causes an 1,4,5-inositol trisphosphate-dependent rise in intracellular cytoplasmic calcium that is inhibited by PMA, a potent activator of protein kinase C. Consequently, protein kinase C is widely believed to mediate feedback inhibition of TCR-activated phospholipase C. We have now extended these studies to normal unblasted human CD4+ T lymphocytes, examining the PMA sensitivity of both the TCR complex-mediated release of total inositol-phosphates and the resynthesis of the parent phosphoinositides. In contrast to Jurkat, in which PMA inhibited release of 1,4,5-inositol trisphosphate by 60% and total inositolphosphates by 40% (50% inhibitory concentration, 5.6 nM), normal cells displayed a marked increase in anti-CD3-induced phosphatidylinositol (PI) cycling in the presence of PMA. Both total inositolphosphate release and PI resynthesis were maximally elevated (88% and 342%, respectively) by a PMA concentration that also optimally supported a subsequent proliferative response; the ED50 was at least 11.7-fold lower than that for the inhibitory effect of PMA on breakdown of total Jurkat PI. A PKC nonactivating phorbol ester had no effect. If anti-CD3 was replaced by the mitogenic lectin PHA, PI resynthesis was similarly up-regulated by PMA in these highly purified cells. The PMA up-regulatory phenomenon was not a simple consequence of cell blastogenesis, inasmuch as there was no early effect on the non-signaling-associated phosphatidylethanolamine compartment after CD3 stimulation. Thus, PKC activation appears to accelerate TCR-linked PI metabolism in normal Th cells, in contrast to the feedback inhibitor paradigm observed in Jurkat and other tumor cell systems.
APA, Harvard, Vancouver, ISO, and other styles
5

Treves, S., F. Di Virgilio, V. Cerundolo, P. Zanovello, D. Collavo, and T. Pozzan. "Calcium and inositolphosphates in the activation of T cell-mediated cytotoxicity." Journal of Experimental Medicine 166, no. 1 (July 1, 1987): 33–42. http://dx.doi.org/10.1084/jem.166.1.33.

Full text
Abstract:
Reports from a number of laboratories have shown that mAbs against the T3-Ti receptor complex cause an increase in cytosolic-free Ca2+ [( Ca2+]i) and the hydrolysis of phosphatidylinositolbisphosphate (PIP2) in CTLs. In the present report we show that activation of CTLs by their specific targets causes: (a) release of Ca2+ from intracellular stores; (b) transient formation of inositol trisphosphate (InsP3); and (c) an increased permeability to Ca2+ of CTL plasma membrane. Killing of unrelated targets could be induced by cocentrifugation of the unrelated targets with CTLs in the presence of A23187 or PMA. We conclude that: (a) activation of CTLs by specific antigens triggers the generation of the same intracellular mediators generated by stimulation of lymphocytes with anti-T3-Ti receptor antibodies and/or with polyclonal mitogens; and (b) intracellular signals that mediate the delivery of the lethal hit by CTLs are indistinguishable from those that induce cell proliferation.
APA, Harvard, Vancouver, ISO, and other styles
6

Bogdanowicz, P., and JP Pujol. "Rôle des inositolphosphates glycanes dans la signalisation intracellulaire : relations avec la pathologie." médecine/sciences 17, no. 5 (2001): 577. http://dx.doi.org/10.4267/10608/1970.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Sorokina, I. V., I. V. Borzenkova, M. S. Myroshnychenko, O. M. Pliten, O. A. Omelchenko, and A. V. Simachova. "Content of inositolphosphates in blood and urine is a marker of renal pathology." Problems of Uninterrupted Medical Training and Science 2017, no. 1 (April 2017): 58–61. http://dx.doi.org/10.31071/promedosvity2017.01.058.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Borda, Enri, Graciela Stranieri, and Leonor Sterin-Borda. "H1-Receptor activation triggers the endogenous nitric oxide signalling system in the rat submandibular gland." Mediators of Inflammation 11, no. 6 (2002): 337–43. http://dx.doi.org/10.1080/0962935021000051520.

Full text
Abstract:
Background: Histamine is released from mast cells by immunologic and non-immunologic stimuli during salivary gland inflammation, regulating salivary secretion. The receptor-secretory mechanism has not been studied in detail.Aims: The studies reported were directed toward elucidating signal transduction/second messenger pathways within the rat submandibular gland associated with 2-thiazolylethylamine (ThEA)-induced H1-receptor responses.Materials and methods: To assess the H1receptor subtype expression in the rat submandibular gland, a radioligand binding assay was performed. The study also included inositolphosphates and cyclic GMP accumulation, protein kinase C and nitric oxide synthase activities, and amylase release.Results: The histamine H1receptor subtype is expressed on the rat submandibular gland with high-affinity binding sites. The ThEA effect was associated with activation of phosphoinositide-specific phospholipase C, translocation of protein kinase C, stimulation of nitric oxide synthase activity and increased production of cyclic GMP. ThEA stimulation of nitric oxide synthase and cyclic GMP was blunted by agents able to interfere with calcium movilization, while a protein kinase C inhibitor was able to stimulate ThEA action. On the other hand, ThEA stimulation evoked amylase release via the H1receptor but was not followed by the L-arginine/nitric oxide pathway activation.Conclusions: These results suggest that, apart from the effect of ThEA on amylase release, it also appears to be a vasoactive chemical mediator that triggers vasodilatation, modulating the course of inflammation.
APA, Harvard, Vancouver, ISO, and other styles
9

Ticchioni, M., C. Aussel, J. P. Breittmayer, S. Manié, C. Pelassy, and A. Bernard. "Suppressive effect of T cell proliferation via the CD29 molecule. The CD29 mAb 1 "K20" decreases diacylglycerol and phosphatidic acid levels in activated T cells." Journal of Immunology 151, no. 1 (July 1, 1993): 119–27. http://dx.doi.org/10.4049/jimmunol.151.1.119.

Full text
Abstract:
Abstract We had previously reported that the CD29 mAb "K20," presented in a soluble form, blocks peripheral T cell proliferation/activation induced by a CD3 mAb. To better characterize the negative signal delivered by soluble K20, we have investigated its effects on the phospholipid metabolism, both in Jurkat and CD4+ T cells. In CD3-activated T cells, K20 inhibited the increase of diacylglycerol (DAG) and phosphatidic acid levels, but did not modify phosphatidylinositol 4,5-bisphosphate levels, cytosolic Ca2+ raise, and inositolphosphates formation, indicating that K20 did not inhibit phosphatidylinositol 4,5-bisphosphate hydrolysis by phospholipase C-gamma. Moreover, in these conditions, K20 increased phosphatidylethanolamine levels, without variation of phosphatidylcholine, phosphatidylserine, and phosphatidylinositol, suggesting that K20 specifically increased the phosphatidylethanolamine biosynthesis from DAG. Thus, the effects of K20 on DAG and phosphatidic acid levels resulted from an accelerated catabolism rather than from a defect of synthesis. That K20 acts solely at an early step of T cell activation, namely before the binding of IL-2 to its receptor, is supported by the observation that adding exogenous rIL-2 increased proliferation in spite of K20. These results suggest that the beta 1 integrin molecules interact with the membrane phospholipid metabolism and they appear to be the hallmark of a peculiar negative pathway of T cell activation, likely to play an important regulatory role mediated via the T cell integrin molecules.
APA, Harvard, Vancouver, ISO, and other styles
10

FRAGOSO, GABRIELA, and ANA MARÍA LÓPEZ-COLOMÉ. "Excitatory amino acid-induced inositol phosphate formation in cultured retinal pigment epithelium." Visual Neuroscience 16, no. 2 (March 1999): 263–69. http://dx.doi.org/10.1017/s0952523899162072.

Full text
Abstract:
Excitatory amino acid (EAA)-induced production of inositolphosphates (IPs) was studied in primary cultures of chick retinal pigment epithelium (RPE) following in vitro incorporation of [3H] myo-inositol. Glutamic acid (L-glu) significantly increased [3H]-IPs accumulation (215%). L-glu agonists stimulated [3H]IPs accumulation in the following order of efficiency: N-methyl-D-aspartate (NMDA) ≥ L-glu > quisqualate ≥ kainate > (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (ACPD). Stimulation was dependent on external Ca2+. The NMDA-induced response was blocked by (+)-5-methyl-10,11-dihydro-5H-dibenzo-cyclohepten-5,10-imine maleate (MK-801) and 3-(2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (CPP) and was decreased by the L-Ca2+-channel blockers verapamil and nifedipine as well as by dantrolene. The metabotropic glutamate receptor (mGluR) antagonist (+)-α-methyl-4-carboxyphenylglycine (+)MCPG inhibited 3,5-dihydroxyphenylglycine (DHPG) and ACPD-induced stimulation, which demonstrates the presence in RPE of mGluRs 1 and/or 5, as well as NMDA receptors coupled directly, or through the influx of external Ca2+, to phospholipase C activation. L-glu agonists showed no effect either on basal level of intracellular cyclic adenosine monophosphate, nor on forskolin- or carbachol-induced stimulation of adenylyl cyclase. Since L-glu is released from the retina upon illumination, and receptors for this compound are present in RPE, the activation of the inositide pathway could be involved in the regulation of retina-RPE interaction, which is essential for the visual process.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "Inositolphosphates"

1

Belgaroui, Nibras. "Dissection moléculaire et physiologique du rôle de l'acide phytique dans la réponse des plantes aux stress de l'environnement." Thesis, Montpellier, SupAgro, 2016. http://www.theses.fr/2016NSAM0037.

Full text
Abstract:
La faible disponibilité du phosphore (P) constitue une contrainte majeure à la croissance et au développement des cultures végétales à l’échelle mondiale. En fait, jusqu’à 95% du P total dans les sols agricoles existe sous forme de P organique dont le composant le plus prépondérant est l’acide phytique, qui n’est disponible aux plantes que s’il est hydrolysé par des enzymes spécifiques appelées les phytases. Nous nous sommes intéressés ici à l’étude d’une phytase PHY-US417 de la souche de Bacillus subtilis US417 afin de tester sa capacité à améliorer la mobilisation du P à partir de l’AP chez les plantes d’Arabidopsis thaliana. Nous avons démontré dans un premier temps que la surexpression chez Arabidopsis d'une forme intracellulaire de la phytase PHY-US417 a entrainé une diminution de 40% le taux d’AP dans les graines. Des analyses physiologiques ont révélé que la surexpression de cette phytase améliore la croissance des plantes après un stress par manque de P, grâce à l’augmentation du niveau intracellulaire du Pi et du sulfate. Remarquablement, une plus forte mobilisation du fer a été observée au cours de la germination chez ces lignées transgéniques. En outre, la perception du Pi extérieur suite à des changements dans les profils d'expression de certains gènes induits par la carence en phosphate. Ces résultats indiquent que l’AP constitue non seulement une forme de stockage de P au niveau des graines mais agit aussi comme une molécule de signalisation qui régule l’interaction entre l’homéostasie du phosphore et du sulfate.Ces lignées transgéniques nous ont conduit à conclure aussi que l'AP et ses dérivés en inositol phosphates pourraient être impliqués aussi dans l'interconnexion entre les voies régulant l'homéostasie le P et celle du Zn. Ces mêmes composants semblent également jouer un rôle de régulateur positif dans la réponse des plantes au stress osmotique et ce via une stimulation des activités antioxydantes. Dans un deuxième temps, l'effet de la sécrétion de la phytase PHU-US417 par les racines d'Arabidopsis a été étudié. Les résultats obtenus ont montré que la forme extracellulaire ePHY-US417 a été capable d'hydrolyser l'AP utilisé comme seul source de Pi dans le milieu extérieur. En conséquence, ces lignées surexprimant ePHY-US417 ont une meilleure acquisition du Pi et une meilleure croissance. Enfin cette amélioration de la croissance dans des conditions de carence en Pi a concerné aussi d'autres plantes d'Arabidopsis ou de tabac co-cultivées avec les lignées sécrétant la phytase ePHY-US417. Une telle avancée montre que les phytases secrétées pourraient avoir un fort potentiel de valorisation via le développement de nouvelles pratiques de cultures associées qui favorisent une agriculture durable en limitant l'usage intensif des engrais phosphatés
The low availability of phosphorus (P) is a major constraint to growth and development of vegetable crops worldwide. In fact, up to 95% of total P in agricultural soils exists as organic P where phytic acid is the most dominant component, that is not available to plants unless hydrolyzed by specific enzymes called phytases. We are particularly interested to study a microbial phytase PHYC of Bacillus subtilis US417 strain (Farhat et al., 2008) to test its ability to enhance the mobilization of P from PA in Arabidopsis thaliana plants. Firstly, we have demonstrated in this work that overexpression of an intracellular form of phytase PHY-US417 in Arabidopsis resulted in reduction of PA levels in the grain.Physiological analyzes showed that overexpression of this phytase improves plant growth after P deficiency, by increasing the intracellular level of Pi and sulfate. Remarkably, a stronger mobilization of iron was observed during germination in these transgenic lines. In addition, the perception of the outside Pi result of changes in the expression profiles of genes induced by phosphate deficiency. These results show that the AP is not only a storage form in seeds but also acts as a signaling molecule that regulates the interaction between the phosphorus and sulfate homeostasis.These transgenic lines also led to conclude that the AP and its derivatives inositol phosphates may also be involved in the interconnection between the pathways regulating homeostasis of P and Zn. These components also appear to play a role as a positive regulator in the plant response to osmotic stress and this via stimulation of antioxidant activities.In a second step, the effect of the secretion of the PHY-US417 phytase by Arabidopsis roots was studied. The results showed that the extracellular form ePHY-US417 was able to hydrolyze the PA used as the sole P source of Pi in the external environment. Therefore these lines over ePHY-US417 have a better acquisition of Pi and better growth.Finally, this improved growth in Pi deficiency conditions has also affected other plants Arabidopsis or tobacco co-cultivated with plants secreting phytase. Such a step shows that the secreted phytase could have a significant upside potential through the development of new ways of "intercropping" that support sustainable agriculture by limiting the intensive use of phosphate fertilizers
APA, Harvard, Vancouver, ISO, and other styles
2

Kincses, Monika. "Biosynthese von aktiviertem Inositolphosphat." [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=964969564.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Nalaskowski, Marcus M. "Multifunktionelle Proteine im Inositolphosphat-Metabolismus." [S.l. : s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=970027591.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Sartelet, Hervé. "Glycosylphosphatidylinositol et inositolphosphate glycanne, ancrage et signalisation." Reims, 1996. http://www.theses.fr/1996REIMS024.

Full text
Abstract:
L'etude de la regulation croisee des voies de signalisation dans les cellules thyroidiennes de porc a fait apparaitre, a cote des voies classiques, une voie originale faisant intervenir un precurseur lipidique, le glycosylphosphatidylinositol (gpi) et son produit d'hydrolyse, l'inositolphosphate glycanne (ipg). L'ipg a ete decrit, dans notre laboratoire ou en collaboration avec d'autres equipes, comme un second messager pour des hormones ou des facteurs de croissance (tsh, erythropoietine, tgf beta et insuline). Dans ce travail, nous avons cherche a approfondir nos connaissances sur le systeme gpi/ipg dans les cellules thyroidiennes de porc. Nous avons montre que l'ipg est capable de stimuler l'incorporation de thymidine tritiee dans les thyrocytes de porc mais egalement dans les fibroblastes de rat et dans les chondrocytes articulaires de lapin. Ceci montre qu'il existe une certaine interspecificite de l'ipg qui s'avere etre une molecule importante dans les processus de proliferation. Nous nous sommes ensuite interesses au gpi, precurseur de l'ipg mais egalement molecule d'ancrage de certaines proteines aux membranes cellulaires. Pour cela, nous avons purifie jusqu'a homogeneite du gpi libre, c'est a dire non ancre a une proteine. L'etude de la composition en monomeres de sa partie glycannique indique que ce gpi possede une molecule de glucosamine non n-acetylee pour environ trois mannoses, du galactose et du glucose en plus faible proportion. Nos resultats nous permettent de penser que le gpi libre n'est pas le precurseur direct de l'ipg mais plutot une reserve de gpi pour l'ancrage de proteines. Dans la derniere partie de notre travail, nous avons mis en evidence la presence de thyroglobuline d'une part ancree par le gpi et d'autre part possedant une molecule d'ipg dans sa structure. Cette decouverte est essentielle dans la comprehension du trafic bidirectionnel de la thyroglobuline dans les cellules thyroidiennes de porc
APA, Harvard, Vancouver, ISO, and other styles
5

Gün, Ümit [Verfasser]. "Synthese von Aminokonduritolen und Inositolphosphat-Analoga / Ümit Gün." Wuppertal : Universitätsbibliothek Wuppertal, 2015. http://d-nb.info/108079655X/34.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Plettenburg, Oliver. "Ein neuartiger, flexibler Zugang zu myo-Inositolphosphaten und -derivaten aus para-Benzochinon." [S.l. : s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=961473231.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Erdmann, Nina Martine [Verfasser], and Sabine [Akademischer Betreuer] Windhorst. "Wirkung von Inositolphosphaten auf die Blutgerinnung / Nina Martine Erdmann ; Betreuer: Sabine Windhorst." Hamburg : Staats- und Universitätsbibliothek Hamburg, 2019. http://d-nb.info/1181947162/34.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Erdmann, Nina Martine Verfasser], and Sabine [Akademischer Betreuer] [Windhorst. "Wirkung von Inositolphosphaten auf die Blutgerinnung / Nina Martine Erdmann ; Betreuer: Sabine Windhorst." Hamburg : Staats- und Universitätsbibliothek Hamburg, 2019. http://d-nb.info/1181947162/34.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Dallmann, Guido. "Untersuchungen zum Stoffwechsel von Inositolphosphaten in Dictyostelium discoideum Anreicherung und Charakterisierung von Phosphatasen unterschiedlicher Spezifität /." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=982382049.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Martiny, Laurent. "Les phosphoinositides dans les cellules thyroidiennes porcines en culture. Production et role d'un inositolphosphate oligosaccharide." Reims, 1990. http://www.theses.fr/1990REIMS011.

Full text
Abstract:
L'etude du metabolisme des phosphoinositides dans les cellules thyroidiennes porcines en culture montre que: la tsh induit un effet chronique, camp dependant, sur le metabolisme des phosphoinositides. Par contre, il n'y a pas d'effet aigu de l'hormone sur la liberation des inositolphosphates. La recherche d'une autre voie de signalisation susceptible d'expliquer ces resultats montre: 1) la presence d'inositolphosphooligosaccharide (in-pos) produit de l'hydrolyse du glycosylphosphatidylinositol (gly-pi) par une phospholipase c specifique; 2) la production d'in-pos et l'hydrolyse simultanee du gly-pi en reponse a une stimulation aigue par la tsh; 3) l'existence d'effets biologiques de l'in-pos sur la production de camp et sur l'organification de l'iodure; 4) l'implication de proteines gtp dependantes dans le mode d'action de l'in-pos; 5) la possibilite d'ancrage de proteines par le gly-pi dans les cellules thyroidiennes; 6) l'in-pos et le gly-pi semblent donc devoir occuper une place importante dans le mode d'action de la tsh sur le metabolisme thyroidien
APA, Harvard, Vancouver, ISO, and other styles
More sources

Book chapters on the topic "Inositolphosphates"

1

Verhaert, Peter, Hilde Walgraeve, and Roger Downer. "Metabolism of Inositolphosphates in the Cerebral Ganglia of the American Cockroach, Periplaneta americana L." In Insect Neurochemistry and Neurophysiology · 1989 ·, 275–79. Totowa, NJ: Humana Press, 1990. http://dx.doi.org/10.1007/978-1-4612-4512-4_24.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Hartmann, H., A. Eckert, and W. E. Müller. "Ca2+-Freisetzung und Inositolphosphat-Akkumulation in stimulierten Human-Lymphozyten und -Granulozyten." In Biologische Psychiatrie der Gegenwart, 756–60. Vienna: Springer Vienna, 1993. http://dx.doi.org/10.1007/978-3-7091-9263-4_175.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

van Calker, D., M. Bohus, P. Gebicke-Härter, H. Hecht, H. J. Wark, and M. Berger. "Sensitivität des Inositolphosphat/Ca2+-second messenger Systems bei affektiven Störungen: Pathogenetischer Faktor und Angriffspunkt prophylaktischer Lithiumtherapie?" In Aktuelle Perspektiven der Biologischen Psychiatrie, 778–82. Vienna: Springer Vienna, 1996. http://dx.doi.org/10.1007/978-3-7091-6889-9_184.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "Inositolphosphates"

1

Medini, L., P. Maderna, E. Tremoli, and C. Galli. "PLATELETS FROM TYPE IIA HYPERCHOLESTEROLEMIC PATIENTS GENERATE MORE INOSITOLPHOSPHATES AFTER THROMBIN STIMULATION IN COMPARISON WITH THOSE OF NORMAL SUBJECTS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643415.

Full text
Abstract:
Functional and biochemical responses of platelets to stimulating agents have been reported to be amplified in several pathological conditions, including hyperlipidemia. Enhanced aggregation and thromboxane formation are, e.g. frequently observed in the presence of high plasma cholesterol levels (type Ila hypercholesterolemia). Stimulation of phosphoinositides breakdown through specific phosphohydrolases (phospholipase C) resulting in the formation of inositolphosphates (IPs), is one of the early events in platelet activation. A study was thus designed in order to investigate IPs generation in thrombin stimulated platelets from type Ila hypercholesterolemic patients in comparison with a group of normocholesterolemic subjects. Preparation of washed platelets, prelabelling with 2[3H] myo inositol and separation of lipid and water soluble inositides was carried out according to Watson et al (1). Product generation (inositoltrisphosphate, IP3; inositolbisphosphate, IP2; inositol mono phosphate, IP) in relation to the 2[3H) inositol incorporated in phosphoinositides was evaluated at 10 and 90 s after platelet stimulation with 1 U/ml NIH in the presence of 10 mM lithium chloride. The major differences found in platelets from type Ila patients in comparison with those of controls were the following:1) in non stimulated platelets a greater incorporation of myoinositol in phosphoinositides and lower levels of labelled IP2; 2) after stimulation, the levels of all labelled IPs were significantly greater, and those of IP2 were double than in controls. In particular the percent increment of IP2 over basal values in platelets of type Ila patients was more than two fold greater. It is concluded that the enhanced generation of IPs in platelets from type IIa patients, following thrombin stimulation, may contribute to the greater sensitivity to agonists of the aggregatory process in this pathological condition.1) Watson P.S., McConnell R.T. and Lapetina E.G., J. Biol. Chem.21:13199, 1984
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'Inositolphosphates' for particular source types:
Journal articles Dissertations / Theses
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography