Academic literature on the topic 'Maximum bactericidal concentration (MBC)'
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Journal articles on the topic "Maximum bactericidal concentration (MBC)"
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E.I., Okoye. "Qualitative and Quantitative Phytochemical Analysis and Antimicrobial Screening of Solvent Extracts of Amaranthus hybridus (Stem and Leaves)." Chemistry Research Journal 3, no. 1 (2018): 9–13. https://doi.org/10.5281/zenodo.13924983.
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Qualitative and quantitative phytochemical screening of <em>Amaranthus hybridus</em> showed the presence of these phytocompounds. Alkaloids 4.00% tannins 3.40%, saponins 18.00%, flavonoids 19.20 %, terpenoids 3.03 % and steroid 7.98 %. Ethanolic, n-hexane and aqueous extracts of <em>Amaranthus hybridus</em> were found to inhibit six test micro-organisms. They are <em>Pseudomonas aeroginosa, Salmonella typhi, Escherichia coli, Apsergillus flavus, Aspergillus niger </em>and<em> Candida albican.</em> The minimum inhibitory concentration (MIC), maximum bactericidal concentration (MBC)/MFC for the three solvent extracts were found for each bacterium and fungus.
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Ibarra-Arán, Juana-Cristina, Douglas Rodríguez-Martínez, Eugenio Rodríguez-González, and Jesús-Roberto González-Castillo. "In vitro evaluation of bactericidal effect of silver and gold-silver nanoparticles coated with silicon dioxide on Xanthomonas fragariae." MRS Advances 2, no. 49 (2017): 2683–88. http://dx.doi.org/10.1557/adv.2017.528.
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ABSTRACTIn this work it was evaluated the bactericidal effect of silver and silver-gold nanoparticles coated with silicon dioxide on Xanthomonas fragariae. Nanoparticles were synthesized by combining laser ablation and chemical synthesis techniques. Irradiating the samples at the same wavelength where nanoparticles exhibit its maximum absorbance is possible to photo induce the bactericidal effect by detonating the surface plasmon resonance. The results showed that the minimum bactericidal concentration (MBC) of silver nanoparticles was 16 μM (referred to [AgNO3]) and for silver-gold nanoparticles were 32 μM (referred to [AgNO3-HAuCl4]) at 60 minutes of irradiation.
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Pathirana, Hansani N. K. S., Sudu H. M. P. Wimalasena, Benthotage C. J. De Silva, Sabrina Hossain, and Gang-Joon Heo. "Antibacterial activity of lime (Citrus aurantifolia) essential oil and limonene against fish pathogenic bacteria isolated from cultured olive flounder (Paralichthys olivaceus)." Archives of Polish Fisheries 26, no. 2 (2018): 131–39. http://dx.doi.org/10.2478/aopf-2018-0014.
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Abstract The antibacterial activity of lime (Citrus aurantifolia) essential oil (LEO) and limonene was tested against seven Gram-negative and nine Gram-positive fish pathogenic bacteria isolated from cultured olive flounder, Paralichthys olivaceus (Temminck & Schlegel) in Korea. Limonene was >99% concentrated and LEO consisted of eleven chemical compounds including 56.22% of limonene. Disk diffusion assay, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) tests were done. LEO and limonene inhibited the growth of both Gram-negative and Gram-positive bacteria. LEO and limonene (MBC/MIC= 2-8) were both bactericidal and bacteriostatic for the strains tested. In every fish pathogenic bacteria, the inhibition zone diameter (IZD) increased in proportion to the oil concentration and the maximum effect was found at 100% (V/V) concentrations of LEO and limonene. The antibiogram pattern indicated that all the bacterial strains, excluding three strains of S. iniae (S186, S530, and S131), showed resistance to one or more antibiotics. The percentage of the relative inhibition zone diameter (RIZD %) exhibited high values at higher concentrations of all the agents. Since antibacterial activities of LEO and limonene were considerably effective against fish pathogenic bacteria, they could be used as alternatives to treat bacterial infections in aquaculture.
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A. Alwathnani, Hend. "Antibacterial Activity of Aqueous Extracts of Artemisia species against some Pathogenic Bacteria." Biosciences, Biotechnology Research Asia 14, no. 2 (2017): 621–24. http://dx.doi.org/10.13005/bbra/2486.
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ABSTRACT: The present study was lead with a view to evaluate the antibacterial potentials of aqueous extracts of Artemisia species against human pathogenic bacteria: Escherichia coli (E.coli), Pseudomonas aeruginosa (Ps. aeruginosa) , Staphylococcus aureus (Staph. aureus) and Enterococcus faecalis (Entero. faecalis) by two methods, first method was minimum inhibitory concentration (abbreviation: MIC) and sound method was minimum bactericidal concentration (abbreviation: MBC). Three crude plants extracts namely Artemisia monosperma, Artemisia cina and Artemisia argyi were found to show potential antibacterial properties against the isolated human pathogenic bacterial isolates. The results of MIC and MBC values of Artemisia monosperma for Enterococcus faecalis strains were at least concentration almost 2.3 and 4.1 mg/ml respectively, while MIC and MBC of Artemisia cina for Escherichia coli were highest concentration 3.9 and 4.9 mg/ml respectively. Moreover, MIC and MBC values for Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus faecalis strains was correspondent with MIC and MBC values of Escherichia coli for aqueous extracts of Artemisia species meanwhile it is Artemisia cina which showed maximum activity against Escherichia coli. Among the Artemisia species tested, Artemisia cina showed the most promising result. Form these results prove the antibacterial potential of the plants and hence provide support for the use of them in traditional medicine.
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Putri, Chany Mony Dwiayu, Diani Prisinda, and Yuti Malinda. "<strong>The MIC and MBC of calcium hydroxide medicament against bacteria that cause chronic periapical abscess in the vulnerable initial 7-days of endodontic treatment</strong>." Padjadjaran Journal of Dentistry 34, no. 1 (2022): 16. http://dx.doi.org/10.24198/pjd.vol34no1.28638.
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Introduction: The primary aetiology of chronic periapical abscesses, including E. faecalis, S. mutans, S. sanguinis, and P. gingivalis, can be eliminated using intracanal medicaments such as calcium hydroxide. The purpose of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) value of calcium hydroxide paste in the vulnerable initial 7-day of treatment against E. faecalis ATCC 29212, S. mutans ATCC 25175, S. sanguinis ATCC 10556, and P. gingivalis ATCC 33277. Methods: An in-vitro laboratory experiment using a spectrophotometer as a microdilution method was conducted to determine bacteria's MIC and MBC values on calcium hydroxide paste. The sample used in this study was four bacteria using intracanal medicament of calcium hydroxide (Ca(OH)2), incubated for seven days at 37°C, and then bacterial growth was observed. The cell inhibition percentage was calculated using optical density measurements to determine the MIC value. The low MIC and MBC were defined as sensitive bacteria to calcium hydroxide. Results: Calcium hydroxide paste against E. faecalis (ATCC 29212) with MIC values at a concentration of 750 μg/ml and MBC values at a concentration of 96,000 μg ml; S. mutans (ATCC 25175) with MIC value at a concentration of 3,000 ug/ml and MBC value at a concentration of 48,000 ug/ml; S. sanguinis (ATCC 10556) with MIC value at a concentration of 3,000 ug/ml and MBC value at a concentration of 6,000 ug/ml; P. gingivalis (ATCC 33277) with MIC value at a concentration of 6,000 ug/ml and MBC value at a concentration of 48,000 ug ml. Conclusions: Calcium hydroxide can inhibit bacterial growth activity. E. faecalis (ATCC 29212) and S. sanguinis (ATCC 10556) are more sensitive to calcium hydroxide paste than other bacteria, with the lowest MIC and MBC on seven days of incubation since the maximum calcium and hydroxyl ions are released.
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Schmidt, H., A. Dalhoff, K. Stuertz, et al. "Moxifloxacin in the Therapy of Experimental Pneumococcal Meningitis." Antimicrobial Agents and Chemotherapy 42, no. 6 (1998): 1397–401. http://dx.doi.org/10.1128/aac.42.6.1397.
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ABSTRACT The activity of moxifloxacin (BAY 12-8039) against aStreptococcus pneumoniae type 3 strain (MIC and minimum bactericidal concentration [MBC] of moxifloxacin, 0.06 and 0.25 μg/ml, respectively; MIC and MBC of ceftriaxone, 0.03 and 0.06 μg/ml, respectively) was determined in vitro and in a rabbit model of meningitis. Despite comparable bactericidal activity, 10 μg of moxifloxacin per ml released lipoteichoic and teichoic acids less rapidly than 10 μg of ceftriaxone per ml in vitro. Against experimental meningitis, 10 mg of moxifloxacin per kg of body weight per ml reduced the bacterial titers in cerebrospinal fluid (CSF) almost as rapidly as ceftriaxone did (mean ± standard deviation, −0.32 ± 0.14 versus −0.39 ± 0.11 Δlog CFU/ml/h). The activity of moxifloxacin could be described by a sigmoid dose-response curve with a maximum effect of −0.33 ΔlogCFU/ml/h and with a dosage of 1.4 mg/kg/h producing a half-maximal effect. Maximum tumor necrosis factor activity in CSF was observed later with moxifloxacin than with ceftriaxone (5 versus 2 h after the initiation of treatment). At 10 mg/kg/h, the concentrations of moxifloxacin in CSF were 3.8 ± 1.2 μg/ml. Adjunctive treatment with dexamethasone at 1 mg/kg prior to the initiation of antibiotic treatment only marginally reduced the concentrations of moxifloxacin in CSF (3.3 ± 0.6 μg/ml). In conclusion, moxifloxacin may qualify for use in the treatment ofS. pneumoniae meningitis.
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Rodriguez-Cerrato, Violeta, Cynthia C. McCoig, Ian C. Michelow, et al. "Pharmacodynamics and Bactericidal Activity of Moxifloxacin in Experimental Escherichia coliMeningitis." Antimicrobial Agents and Chemotherapy 45, no. 11 (2001): 3092–97. http://dx.doi.org/10.1128/aac.45.11.3092-3097.2001.
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ABSTRACT Moxifloxacin, an 8-methoxyquinolone with broad-spectrum activity in vitro, was studied in the rabbit model of Escherichia colimeningitis. The purposes of this study were to evaluate the bactericidal effectiveness and the pharmacodynamic profile of moxifloxacin in cerebrospinal fluid (CSF) and to compare the bactericidal activity with that of ceftriaxone and meropenem therapy. After induction of meningitis, animals were given single doses of 10, 20, and 40 mg/kg or divided-dose regimens of 5, 10, and 20 mg/kg twice, separated by 6 h. After single doses, the penetration of moxifloxacin into purulent CSF, measured as percentage of the area under the concentration-time curve (AUC) in CSF relative to the AUC in plasma, was approximately 50%. After single doses of 10, 20, and 40 mg/kg, the maximum CSF concentration (C max) values were 1.8, 4.2, and 4.9 μg/ml, respectively; the AUC values (total drug) were 13.4, 25.4, and 27.1 μg/ml · h, respectively, and the half-life values (t ½) were 6.7, 6.6, and 4.7 h, respectively. The bacterial killing in CSF for moxifloxacin, calculated as the Δlog10 CFU per milliliter per hour, at 3, 6, and 12 h after single doses of 10, 20, and 40 mg/kg were −5.70, −6.62, and −7.02; −7.37, −7.37, and −6.87; and −6.62, −6.62, and −6.62, respectively, whereas those of ceftriaxone and meropenem were −4.18, −5.24, and −4.43, and −3.64, −3.59, and −4.12, respectively. The CSF pharmacodynamic indices of AUC/MBC and C max/MBC were interrelated (r = 0.81); there was less correlation withT > MBC (r = 0.74). In this model, therapy with moxifloxacin appears to be at least as effective as ceftriaxone and more effective than meropenem therapy in eradicatingE. coli from CSF.
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Hajifattahi, Farnaz, Elham Moravej-Salehi, Maryam Taheri, Arash Mahboubi, and Mohammad Kamalinejad. "Antibacterial Effect of Hydroalcoholic Extract ofPunica granatumLinn. Petal on Common Oral Microorganisms." International Journal of Biomaterials 2016 (2016): 1–6. http://dx.doi.org/10.1155/2016/8098943.
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Objectives. This study aimed to assess the effect of hydroalcoholic extract ofPunica granatumLinn. (P. granatum) petal onStreptococcus sanguinis,Streptococcus mutans,Streptococcus salivarius,Streptococcus sobrinus,andEnterococcus faecalis.Materials and Methods. In this in vitro study,P. granatumextract was prepared using powdered petals and water-ethanol solvent. Antibacterial effect of the extract, chlorhexidine (CHX), and ampicillin was evaluated on brain heart infusion agar (BHIA) using the cup-plate method. By assessing the diameter of the growth inhibition zone, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract were determined for the above-mentioned bacteria.Results. Hydroalcoholic extract ofP. granatumpetal had inhibitory effects on the proliferation of all five bacterial strains with maximum effect onS. mutanswith MIC and MBC of 3.9 mg/mL. The largest growth inhibition zone diameter belonged toS. sanguinisand the smallest toE. faecalis. Ampicillin and CHX had the greatest inhibitory effect onS. sanguinis.Conclusions. Hydroalcoholic extract ofP. granatumhad a significant antibacterial effect on common oral bacterial pathogens with maximum effect onS. mutans, which is the main microorganism responsible for dental plaque and caries.
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Okwara, V.C, H.A. Njoku, and K.R Oleru. "Antimicrobial Effects of Garlic and Ginger Plants Extracts on Certain Clinical Sample." International Journal of Spectrum Research in Medical and Clinical Practice (IJSRMCP 1, no. 1 (2025): 1–7. https://doi.org/10.5281/zenodo.15163399.
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The antibacterial properties of n-hexane and methanol extracts from ginger and garlic wereevaluated in vitro against Klebsiella pneumoniae, Escherichia coli, Salmonella enterica,, andShigella dysenteriae using the agar well diffusion method. Phytochemical analysis indicated thepresence of flavonoids, saponins, carbohydrates, alkaloids, and triterpenes in the extracts. Thegarlic extracts, both n-hexane and methanol, demonstrated the highest effectiveness against S.dysenteriae, achieving maximum inhibition zones of 27 mm at 40 mg/ml and 29 mm at 80 mg/ml.In contrast, the ginger extracts were more effective against E. coli, with maximum inhibitionzones of 16 mm at 40 mg/ml and 19 mm at 80 mg/ml. The minimum inhibitory concentration(MIC) of the methanol extract for the tested organisms ranged from 5 to 20 mg/ml, while then-hexane extract showed MIC values between 2.5 and 10 mg/ml. The methanol extract of gingerhad MIC values ranging from 10 to 40 mg/ml, and the n-hexane extract had values between 10and 20 mg/ml. The minimum bactericidal concentration (MBC) of the methanol garlic extractwas between 10 and 40 mg/ml, while the n-hexane garlic extract had MBC values ranging from2.5 to 20 mg/ml. The methanol ginger extract's MBC values were between 10 and 40 mg/ml, andthe n-hexane ginger extract had MBC values between 10 and 20 mg/ml. The findings of this studyindicate that these extracts possess antibacterial activity against the tested organisms,suggesting their potential for drug development.
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Akanmu, A. O., H. H. Yunus, S. T. Balogun, O. A. Sodipo, L. M. Paul, and I. Gulani. "Antibacterial Activity of Aqueous and Ethanol Fruit Extracts of Cucumis sativus Linn. Against Selected Microorganisms at the University of Maiduguri Teaching Hospital, Maiduguri." Sahel Journal of Veterinary Sciences 18, no. 2 (2021): 17–22. http://dx.doi.org/10.54058/saheljvs.v18i2.222.
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Cucumber (Cucumis sativus Linn) [Cucurbitaceae] is a famous vegetable crop used for food since ancient times but little is known of its antibacterial potential. This study investigated the phytochemical constituents and antibacterial activities of the aqueous and ethanol fruit extracts of the plant against some clinical isolates (Escherichia coli, Salmonella typhi, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pyogenes, Bacillus subtilis and Corynebacterium spp) using the disc diffusion method. The qualitative phytochemical screening revealed the presence of carbohydrates, cardiac glycosides, terpenoids, cardenolites and flavonoids. Corynebacterium spp was the most susceptible (with maximum inhibition zone of 30.00±0.00 mm). The two extracts had varied antibacterial activity at the same level. At the concentration of 400mg/ml, aqueous extract inhibited S. aureus, Corynebacterium spp, E. coli and K. pneumoniae, while ethanol extract inhibited S. aureus, S. pyogenes, S. typhi and Corynebacterium spp. B. subtilis was inhibited only at the highest (significant) concentration of 600mg/ml (7.00±0.00 mm)[p<0.05]. The MIC value for both extracts against Corynebacterium spp was 25mg/ml. The MBC value against Corynebacterium spp was observed at 50mg/ml and 25mg/ml for the aqueous and ethanol extract respectively. The results suggest that the ethanol extract was bactericidal at low concentration while the aqueous extract was bacteriostatic at low concentration and bactericidal at high concentration against Corynebacterium spp. The difference between the MBC for the two extracts was significant (p<0.05). In conclusion, the results of this study showed that extract of C. sativus could be a potential source of natural antibacterial agent
Dissertations / Theses on the topic "Maximum bactericidal concentration (MBC)"
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Markopoulos, Marjorie M. "Antimicrobial Activity of Fractionated Borohydride-Capped and Electrochemical Colloidal Silver." Wright State University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=wright1515096508634157.
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Hsu, An-Pei, and 許安佩. "Combined Effects of Antibiotics on Minimal Bactericidal Concentration（MBC）." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/04918126791421875880.
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碩士<br>國立交通大學<br>環境工程所<br>90<br>Infections due to Methicillin- resistant Staphylococcus aures (MRSA) continued to be a significant problem in the 1990s. Clinicians now have to face the emergence of strains with reduced susceptibility to vancomycin, i.e., so-called glycopeptide-intermediate Staphylococcus aures (GISA). Therefore, there is a clearly a need for new antibiotic regimens with strong early bactericidal activity against MRSA. In this field, an alternative to the development of new classes of agents could be the use of combinations of well-known compounds.
The aim of our study was to investigate the effect on MBC of different combination between disruption of cell wall biosynthesis antibiotics and inhibition protein biosynthesis antibiotics against clinical MRSA strains. Furthermore, to discuss the synergistic bactericidal effect with Mtox7 model.
From the results of the study, the probability of combination to enormously reduce MBC for Vancomycin—Gentamicin were 87.5﹪ and combination between large slope disruption of cell wall biosynthesis antibiotics and large slope inhibition protein biosynthesis antibiotic were 61.11﹪that could enormously reduce MBC. Comparison with Mtox7 model, the relationship of synergistic bactericidal effect was response addition（RA）.
Book chapters on the topic "Maximum bactericidal concentration (MBC)"
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Nahler, Gerhard. "minimal bactericidal concentration (MBC)." In Dictionary of Pharmaceutical Medicine. Springer Vienna, 2009. http://dx.doi.org/10.1007/978-3-211-89836-9_860.
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Gooch, Jan W. "Minimum Bactericidal Concentration (MBC)." In Encyclopedic Dictionary of Polymers. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_14233.
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Arcani, Bianca Cristina Melo, and Deise Helena Baggio Ribeiro. "Evaluation of the Antimicrobial Potential of Seeds Extracts from Fruits of the Myrtaceae Family." In Métodos Microbiológicos Tradicionais e Avançados para a Segurança de Alimentos. Editora Científica Digital, 2025. https://doi.org/10.37885/250519354.
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Objective: Fruit seeds, often discarded as waste by the food industry, are underutilized source of bioactive compounds. While the antimicrobial activity of Myrtaceae species leaves is well known, few studies have investigated its seeds for this purpose. This study evaluated the antimicrobial potential of hydroalcoholic extracts from seeds of cambuci (Campomanesia phaea), grumixama (Eugenia brasiliensis), jabuticaba (Plinia cauliflora), and pitanga (Eugenia uniflora). The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined to Bacillus cereus, Candida albicans, Escherichia coli, Listeria monocytogenes, Salmonella Typhimurium, Staphylococcus aureus, and Pseudomonas aeruginosa. Method: The ethanolic extracts were concentrated, the antimicrobial potential was assessed using the agar diffusion method, the MICs was determined by microdilution and MBC by drop plate technique. Results: All extracts showed inhibitory potencial. The lowest MICs were determined to grumixama extract (E. coli; 0.04 g/mL), followed by jabuticaba and pitanga (E. coli, L.monocytogenes, S. Typhimurium, and S. aureus; 0.06 g/mL), and cambuci (L. monocytogenes and S. Typhimurium; 0.07 g/mL). Although all extracts showed bactericidal activity at concentrations ranging from 0.06 to 0.95 g/mL, the lowest MBC was observed on pitanga’s extract to E. coli, S. Typhimurium, and S. aureus. Conclusion: The results highlight the potential of seeds as an alternative source of antimicrobial compounds, contributing to the valorization of industrial by-products and reducing the discard of materials with potential technological applications.
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Molina-Gallardo, Axel R., Yesica R. Cruz-Martínez, Julieta Orozco-Martínez, Israel Valencia Quiroz, and C. Tzasna Hernández-Delgado. "The Roles of Farnesol and Farnesene in Curtailing Antibiotic Resistance." In Biotechnology and Drug Development for Targeting Human Diseases. BENTHAM SCIENCE PUBLISHERS, 2024. http://dx.doi.org/10.2174/9789815223163124090005.
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In the extensive domain of “biotechnology and drug development for targeting human diseases”, essential oils have long been revered for their therapeutic potential. Among these, farnesol and farnesene stand out due to their pharmacological attributes. As the challenge of antibiotic resistance intensifies, the scientific community is increasingly exploring the potential of these traditional remedies. Using the KirbyBauer agar diffusion method, a qualitative assessment was conducted on two grampositive and two gram-negative bacterial strains. The broth microdilution technique further determined the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and the sensitizing impacts of these compounds. Both farnesol and farnesene exhibited antibacterial efficacy against all evaluated strains. Their synergistic potential was highlighted when combined with clavulanic acid, cefuroxime, and cefepime. Among these combinations, farnesene paired with cefepime showed pronounced efficacy against Escherichia coli 82 MR, with an MIC of 0.47 μg/mL. In contrast, in the investigation of Staphylococcus aureus 23MR, it was observed that this particular strain exhibited an increased sensitivity when exposed to combinations containing farnesol. Notably, the Minimum Inhibitory Concentration (MIC) was determined to be 0.03 µg/mL in the presence of both antibiotic agents. To gain deeper molecular insights, docking experiments were performed with the βlactamases of E. coli and S. aureus, focusing on the most effective combinations. All tested compounds—cefuroxime, cefepime, farnesene, and farnesol—acted as noncompetitive inhibitors, suggesting their potential mechanisms of action.
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Soares Gettens, Cristina, Thauana Heberle, Nadia Carbonera, Eliezer Avila Gandra, Aline Machado Pereira, and Marcia Arocha Gularte. "ANTIMICROBIAL POTENTIAL AND CHEMICAL AND BIOACTIVE COMPOUNDS IN AGROINDUSTRIAL BY PRODUCTS FROM PEACH." In Ciência e Tecnologia de Alimentos: o avanço da ciência no Brasil - Volume 3. Editora Científica Digital, 2023. http://dx.doi.org/10.37885/230312287.
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Objective: The objective was to determine the antimicrobial potential and evaluate the chemical and bioactive compounds in the by-products of peach syrup production, which were “substandard peaches” and the peach kernel almond and its extracts. Methods: The analyses included total soluble solids, pH, total titratable acidity, centesimal composition, vitamin C, carotenoids, total antioxidant activity, total phenols, antifungal potential, antibacterial activity, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Results: The most concentrated extracts from the peach and peach kernel almond presented higher antioxidant activity in IC50, with levels of 2.66 and 7.88 μg. mL-¹ respectively. The total phenol content was 253.4 mg GAE.100g-¹ for the peach and 29.3 mg GAE.100g-¹ for the almond. The extracts tested showed no antifungal potential; however, the extracts of peach and almond presented antibacterial potential against S. Typhimurim and S. aureus bacteria. The MIC for kernel a l m o n d and peach extracts against S. aureus was 0.75 mg.mL-1 for both, but these did not present CBM. The extracts obtained from the peach by-products did not present antifungal activity, but the peach almond showed an inhibitory effect on S. aureus. Conclusion: The peach and peach kernel almond presented significant antioxidant activity and phenolic compound contents, owning high levels of bioactive compounds.
Conference papers on the topic "Maximum bactericidal concentration (MBC)"
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Mudhar Bintang, Muhammad Ali Khumaini, and Teerapol Srichana. "Antibacterial Activity and <i>In Vitro</i> Cytotoxicity of Colistin in Sodium Deoxycholate Sulfate Formulation." In 5th International Conference and Exhibition on Pharmaceutical Sciences and Technology 2022. Trans Tech Publications Ltd, 2022. http://dx.doi.org/10.4028/p-19rsw3.
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Colistin has its problem with nephrotoxicity despite its capability for combatting multi-drug resistant gram-negative bacteria. Sodium deoxycholate sulfate (SDCS) has been shown to increase the safety profile of nephrotoxic drugs. This study aimed to explore the antimicrobial activity of colistin-SDCS versus free colistin against P. aeruginosa and investigate their cytotoxicity on kidney cells. The colistin micelles were formulated with SDCS followed by lyophilization and their properties were analyzed. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of colistin were determined using the broth microdilution method. The static time-kill kinetics were also employed to monitor the bactericidal activity of formulation over time. The cytotoxicity of formulations was analyzed using MTT colorimetric assay against kidney cells. The colistin-SDCS dry-powder was stable after reconstitution and resulted in 240 to 297 nm in particle size with the zeta potential of -22.8 to -23.4 mV. The colistin-SDCS formulations showed similar antibacterial activity against P. aeruginosa to pure colistin. MIC and MBC were 7.81 and 15.63 µg/mL, respectively. The static-time kill results displayed slightly better bactericidal activity at 24 h. The viability of kidney cells exposure to colistin-SDCS micelle was higher than that of pure colistin.
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Panić, Jovana J., Snežana M. Papović, Teona Teodora V. Borović, et al. "The hydration and antimicrobial properties of selected imidazole-based ionic liquids with a homologous series of chloride oxyanions." In 2nd International Conference on Chemo and Bioinformatics. Institute for Information Technologies, University of Kragujevac, 2023. http://dx.doi.org/10.46793/iccbi23.124p.
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The aim of this work was to get a detailed insight into the ion’s interactions along with the structure-making/structure-breaking tendency that has been retrieved through the perusal of calculated parameters from volumetric measurements for aqueous solutions of three newly synthesized ionic liquids: 1-butyl-3-methylimidazolium chlorite, 1-butyl-3-methylimidazolium chlorate and 1-butyl-3-methylimidazolium perchlorate. Further, the antimicrobial activity of synthesized and commercial (1-butyl-3-methylimidazolium chloride) ionic liquids on certain strains of bacteria and fungi was obtained. Antimicrobial tests were performed using the in vitro microdilution method against isolated strains of Escherichia coli, Staphylococcus aureus, Bacillus cereus bacteria, and the fungus Candida guilliermondii. This method is a rapid, quantitative method for the determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using small amounts of samples (µl) and test compound. Based on the obtained results, the influence of the homologous series of chloride oxyanions on hydration and antimicrobial properties of imidazole-based ionic liquids will be discussed.
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Damayanti, Dini Sri, Putra Agung Dewata, and Nuris Bashirotul Hidayah. "Antibacterial Effect of Aloe vera Ethanol Extract Against the Growth of Bacteria Staphylococcus aureus and Escherichia coli through ZOI Test, MIC and MBC Measurement." In 8th International Students Science Congress. ULUSLARARASI ÖĞRENCİ DERNEKLERİ FEDERASYONU (UDEF), 2024. https://doi.org/10.52460/issc.2024.008.
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Staphylococcus aureus is a gram-positive bacterium while Escherichia coli is a gram-negative bacterium that often causes infections and be used as a bacteriological model for many antibacterial screenings. Aloe vera is known to have active compounds that act as antibacterial, however, the effect of Aloe vera ethanol extract on the inhibition of growth and death of Staphylococcus aureus and Escherichia coli bacteria is not clear yet. Aloe vera was extracted using maceration in ethanol. Phytochemical tests were carried out to de-termine the active compounds in the extract [3]. To measure antibacterial efficacy, a disc diffusion method using concentrations of 3,125%, 6,25%, 12,5%, 25%, 50%, 100%, control (+) and control (-) was done using S. aureus and E. coli. Data analysis using One Way Analysis of Variance (ANOVA) test and then continued Tukey's Post Hock test with a significance of p< 0.05 [4] Minimum Inhibi-tion Concentration (MIC) and Minimum Bactericidal Concentration (MBC) was then determined with the same concentrations. The study's Aloe vera ethanol extract contained flavonoids and alkaloids. The zone of inhibitions diameter on both bacteria at the concentration of 3.125%, 6.25%, 12.5%, 25% and the negative con-trol were 0 mm. At 50% concentration, the diameter was 7.67±0.58 mm, while at 100% concentra-tion was 20.67±1.53 mm. The diameter of tetracycline and amoxicillin were 34 mm and 36 mm, respectively. MIC and MBC of Aloe vera ethanol extract against Staphylococcus aureus at a concen-tration of 12.5% while against Escherichia coli at a concentration of 25%. Aloe vera ethanol extract has antibacterial effects and is able to inhibit and kill Staphylococcus aureus and Escherichia coli bacteria with higher inhibitory power against Staphylococcus aureus than against Escherichia coli.
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de Souza, Heide Mendonça Moreira, Karen Cristina Comin Maldonado, Carla Alciati, and Cristina Lúcia Feijó Ortolani. "Analysis of the antibacterial efficacy of Plantago major L. extract against Streptococcus mutansby microdilution in broth." In VI Seven International Multidisciplinary Congress. Seven Congress, 2024. http://dx.doi.org/10.56238/sevenvimulti2024-050.
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The present study investigated the efficacy of Plantago majorL. (Plantaginaceae) extract against Streptococcus mutans, a bacterium associated with the development of biofilms and dental caries. The main objective was to determine the efficacy of this extract by means of the broth microdilution method, seeking to establish the Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentrations (MBC) of the extract. For this, bacterial suspensions were prepared and incubated with different concentrations of the extract in BHI medium. After 48 hours at 36°C, the turbidity of the samples was evaluated, followed by subculture to check for bacterial growth. The extract of Plantago majorand 1% chlorhexidine were tested, and the MIC of the extract against S. mutansat a concentration of 1.5 x 107 CFU/mL was determined at 25 mg/mL. The results indicate that the extract has significant antimicrobial activity against S. mutans, suggesting that it is a promising natural alternative for the prevention of dental caries. The comparison with chlorhexidine suggests that the extract may be a viable option, especially considering the growing demand for natural antimicrobial agents. In conclusion, this study confirms the efficacy of Plantago major extract against Streptococcus mutans, highlighting its potential as a natural antimicrobial agent. Further research is needed to further explore the mechanisms of action and efficacy of this extract in in vivo models, aiming at its development as a treatment for caries prevention
Reports on the topic "Maximum bactericidal concentration (MBC)"
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Torrungruang, Kitti, and Suchada Chutimawarapun. Antimicrobial activity against periodontopathic bacteria, cell toxicity against gingival fibroblasts and antinflammatory effect of crude extract from mangosteen. Chulalongkorn University, 2006. https://doi.org/10.58837/chula.res.2006.15.
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Extract from mangosteen pericarp has demonstrated various pharmacological activities including anti-bacterial, anti-inflammatory and anti-fungal. It may have potential for the treatment of periodontal disease, which is a chronic inflammatory disease caused by anaerobic bacteria. The aim of this study was 1) to investigate the toxicity of mangosteen extract to human gingival fibroblast, 2) to examine the anti-bacterial activity of the extract against periodontopathic bacteria including P. gingivalis and A. actinomycetemcomitans, and 3) to examine the inhibitory effect of the extract on PGE[subscript2]) production in lipopolysaccharide (LPS)-activated peripheral blood monocytes. The changes in cell viability were observed by inverted phase contrast microscopy and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenytetrazolium bromide (MTT) assay. The extract was not toxic when exposed to fibroblasts for up to 48 hours at the concentration of 200 [M]g/ml or less. The extract exhibited anti-bacterial activity against P. gingivalis, but not A. actinomycetemcomitans. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against P. gingivalis were 20 and 40 [M]g/ml, respectively. The anti-inflammatory activity of the extract was determined by measuring PGE[subscript2] production with enzyme-linked immunosorbent assy (ELISA). The extract significantly inhibited LPS-induced PGE production in a dose-dependent manner. Its inhibitory effect reached maximal level at 10 [M]g/ml. These results suggest that the extract from mangosteen pericarp may be beneficial for periodontal treatment.
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ตัณฑวนิช, ทิพวรรณ, มาลินี จงเจริญใจ, วีณา เคยพุดซา, Tippawan Tantawanich, Malinee Jongjareanjai та Weena Koeypudsa. ประสิทธิภาพของสารสกัดหยาบจากสมุนไพรไทย ในการยับยั้งเชื้อแบคทีเรีย Vibrio spp. ที่ก่อให้เกิดโรคในหอยหวาน (Babylonia areolata Link 1807) : รายงานวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 2013. https://doi.org/10.58837/chula.res.2013.87.
Full textAbstract:
ศึกษาประสิทธิภาพของสารสกัดหยาบสมุนไพรไทยทั้ง 3 ชนิด ได้แก่ ฟ้าทะลายโจร ข่า และใบฝรั่งโดยแบ่งการทดลองออกเป็น 4 ขั้นตอน ขั้นตอนที่ 1 หาค่าความเข้มข้นต่ำสุดในการยับยั้งการเจริญเติบโตของเชื้อแบคทีเรีย Vibrio alginolyticus ที่ทำให้เกิดโรคปากบวมแดงในหอยหวาน (Minimal Inhibitory Concentration: MIC) และค่าความเข้มข้นต่ำสุดในการกำจัดเชื้อแบคทีเรีย (Minimal Bactericidal Concentration: MBC) พบว่าสารสกัดหยาบใบฝรั่งที่สกัดด้วยน้ำกลั่น โดยวิธีการต้มมีประสิทธิภาพในการยับยั้งและกำจัดแบคทีเรีย V.alginolyticus ได้สูงสุด (MIC = 2.73 mg/L และ MBC = 21.88 mg/L) รองลงมาคือ สารสกัดหยาบข่าที่สกัดด้วยเอธานอล 100% และสารสกัดหยาบข่าที่สกัดด้วยน้ำกลั่น (MIC = 2.73 – 10.94 mg/L และ MBC = 21.88 – 43.75 mg/L) ส่วนสารสกัดหยาบฟ้าทะลายโจรมีประสิทธิภาพในการกำจัดแบคทีเรียได้ต่ำสุด (MIC = 5.47 mg/L และ MBC = 87.50 mg/L) ขั้นตอนที่ 2 หาค่าความเข้มข้นของเชื้อแบคทีเรีย V. alginolyticus ที่ทำให้หอยหวานตายลง 50 เปอร์เซ็นต์ ภายในเวลา 24 ชั่วโมง (Median lethal Dose at 24 hr: LD₅₀ at 24 hr) ด้วยวิธีการฉีดเชื้อเข้ากล้ามเนื้อเท้าของหอยหวาน พบว่ามีค่า LD₅₀ at 24 hr เท่ากับ 1.426 x 10⁸ cfu/ml ขั้นตอนที่ 3 ทดสอบหาความเป็นพิษของสารสกัดหยาบจากสมุนไพรไทยทั้ง 3 ชนิดที่ทำให้หอยหวานตายครึ่งหนึ่ง (50 เปอร์เซ็นต์) ภายในเวลา 96 ชั่วโมง โดยวิธีการแช่ พบว่าหอยหวานที่เลี้ยงในสารสกัดหยาบสมุนไพรไทยทั้ง 3 ชนิดจะมีอาการกระวนกระวายและดิ้นไปดิ้นมาทันทีหลังจากแช่ในสารสกัด โดยพบว่าสารสกัดหยาบฟ้าทะลายโจรมีความเป็นพิษระดับสูงที่สุด LD₅₀ at 96 hr = 385.25 มิลลิกรัม/ลิตร) รองลงมาคือ สารสกัดหยาบข่า LD₅₀ 96 hr = 809.39 มิลลิกรัม/ลิตร) และสารสกัดหยาบใบฝรั่งมีความเป็นพิษระดับต่ำที่สุด (LD₅₀ 96 hr = 3,017.11 มิลลิกรัม/ลิตร) และขั้นตอนที่ 4 ศึกษาประสิทธิภาพของสารสกัดหยาบสมุนไพรไทยทั้ง 3 ชนิดในการรักษาโรควิบริโอซีสในหอยหวานด้วยวิธีการแช่ต่อเนื่องเป็นเวลา 14 วัน พบว่าสารสกัดหยาบข่ามีประสิทธิภาพในการป้องกันและรักษาโรคติดเชื้อในหอยหวานได้ดีที่สุด โดยระดับความเข้มข้นของสารสกัดหยาบฟ้าทะลายโจร ข่าและใบฝรั่งที่มีประสิทธิภาพในการป้องกันรักษาโรคสูงสุดคือ ระดับความเข้มข้น 18.75 และ 37.50 มิลลิกรัม/ลิตร ตามลำดับ แต่อัตราการรอดชีวิตของหอยหวานที่แช่ในสารสกัดหยาบสมุนไพรทั้ง 3 ชนิด ไม่มีความแตกต่างกับชุดควบคุมบวกที่ได้รับเชื้อแบคทีเรียแต่ไม่ได้แช่สารสกัดสมุนไพร จากการศึกษานี้พบว่าสารสกัดหยาบใบฝรั่งมีฤทธิ์ในการยับยั้งแบคทีเรีย v. alginolyticus ได้สูงสุด จึงมีความเป็นไปได้ในการนำสารสกัดหยาบจากใบฝรั่งมาประยุกต์ใช้ในวงการเพาะเลี้ยงสัตว์น้ำต่อไป