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Journal articles on the topic 'Methacrylate embedding'

Author: Grafiati

Published: 4 June 2021

Last updated: 6 February 2022

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1

Mollenhauer, Hilton H. "Embedding resins: An historical perspective." Proceedings, annual meeting, Electron Microscopy Society of America 50, no. 2 (1992): 1070–71. http://dx.doi.org/10.1017/s042482010012998x.

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There are several factors that were requisites for the biological applications of electron microscopy. One of these was, clearly, the development of a matrix substance that could infiltrate biological material and encapsulate tissue components so that they could be thin sectioned and examined under an electron beam. Without such matrix substances, examination of tissue as we know it today, would not be possible.Perhaps the start of practical electron microscopy in the context stated above was the application of methacrylate resins for tissue embedment. Methacrylates have a very low viscosity a

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2

Schneider, Jan Philipp, and Matthias Ochs. "Alterations of mouse lung tissue dimensions during processing for morphometry: A comparison of methods." American Journal of Physiology-Lung Cellular and Molecular Physiology 306, no. 4 (2014): L341—L350. http://dx.doi.org/10.1152/ajplung.00329.2013.

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Preservation of original tissue dimensions is an essential prerequisite for morphometric studies. Shrinkage occurring during tissue processing for histology may severely influence the appearance of structures seen under the microscope and stereological calculations. Therefore, shrinkage has to be avoided so that estimates obtained by application of unbiased stereology are indeed unbiased. The present study investigates the alterations of tissue dimensions of mouse lung samples during processing for histology. Different fixatives as well as embedding protocols are considered. Mouse lungs were f

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VARA, D. C. da, A. M. LEAL-ZANCHET, and H. M. LIZARDO-DAUDT. "Histological processing techniques for the study of Dugesiidae development (Platyhelminthes, Tricladida, Paludicola)." Revista Brasileira de Biologia 61, no. 2 (2001): 341–45. http://dx.doi.org/10.1590/s0034-71082001000200019.

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The objective of the present study was to adapt techniques for the histological processing of Dugesiidae cocoons for the study of embryo development. The cocoons were fixed with formalin, SUSA, Bouin or paraformaldehyde/glutaraldehyde and subsequently embedded in Paraplast or glycol methacrylate (Historesin). Paraplast embedding yielded reasonable results only after the cocoon was perforated or fixed for a prolonged period of time using softening techniques with acid solutions. When the SUSA or Bouin fixative and Historesin embedding techniques were used the results were good for light microsc

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4

Hott, Monique, and Pierre J. Marie. "Glycol Methacrylate as An Embedding Medium for Bone." Stain Technology 62, no. 1 (1987): 51–57. http://dx.doi.org/10.3109/10520298709107965.

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5

Skinner, Robert A. "Glycol Methacrylate Double Embedding for Reorienting Problem Blocks." Journal of Histotechnology 10, no. 2 (1987): 117–18. http://dx.doi.org/10.1179/his.1987.10.2.117.

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6

Yeung, Edward C., and Colin K. W. Chan. "Glycol methacrylate: the art of embedding and serial sectioning." Botany 93, no. 1 (2015): 1–8. http://dx.doi.org/10.1139/cjb-2014-0177.

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Glycol methacrylate (GMA) is a popular embedding medium used for high resolution light microscopy. Since the introduction of the GMA embedding method by Feder and O’Brien (1968, Am. J. Bot. 55: 123–142), improvements have been made to this technique. The purpose of our work is to detail some important advancements in the GMA embedding method and discuss different approaches to ensure successful processing and serial sectioning of GMA blocks. The best sectioning method is to combine the use of Ralph glass knives with a rotary microtome with a retraction return stroke. Moreover, softening the po

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7

Baskin, Tobias I. "Butyl-methyl-methacrylate for Immunocytochemistry Through the Light Microscope." Microscopy Today 14, no. 6 (2006): 56–57. http://dx.doi.org/10.1017/s1551929500058910.

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The use of methacrylate monomers for embedding has a venerable history in microscopy. Many formulations have been developed over the years for various purposes, ranging from standard TEM observations to low-temperature embedding. Key parameters include the length of the hydrocarbon chain and the presence and kind of cross linking reagent. In the mixture of butyl and methyl methacrylate (BMM) described here, the monomers are relatively short-chained and there is no cross linker at all. This gives the polymerized material a softness that makes it rather unsuitable for TEM, but on the contrary al

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8

Warren, Kelley C., Kathryn J. Coyne, J. Herbert Waite, and S. Craig Cary. "Use of Methacrylate De-embedding Protocols for In Situ Hybridization on Semithin Plastic Sections with Multiple Detection Strategies." Journal of Histochemistry & Cytochemistry 46, no. 2 (1998): 149–55. http://dx.doi.org/10.1177/002215549804600203.

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New plastic resins are gradually replacing traditional paraffin-embedding in situ hybridization (ISH) strategies. One unique resin that has not been fully investigated or exploited with respect to light microscopic ISH is a methacrylate mixture. The advantage of this resin is its ability to be removed from tissues postsectioning, dramatically increasing hybridization signal compared to that obtained in other plastics. The goal of this study was to evaluate the general applicability of the methacrylate embedding acetone de-embedding (MEADE) technique for ISH investigations. Several high-resolut

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9

GOMESFILHO, J., B. GOMES, A. ZAIA, P. NOVAES, and F. SOUZAFILHO. "Glycol Methacrylate: An Alternative Method for Embedding Subcutaneous Implants." Journal of Endodontics 27, no. 4 (2001): 266–68. http://dx.doi.org/10.1097/00004770-200104000-00005.

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10

Liu, Chung-Ching. "A Simplified Technique for Low Temperature Methyl Methacrylate Embedding." Stain Technology 62, no. 3 (1987): 155–59. http://dx.doi.org/10.3109/10520298709107986.

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11

Fritsehy, W. M., P. O. Gerrits, G. H. J. Wolters, A. Pasma, and R. Van Schilfgaarde. "Glycol Methacrylate Embedding of Algmate-Polylysine Microencapsulated Pancreatic Islets." Biotechnic & Histochemistry 70, no. 4 (1995): 188–93. http://dx.doi.org/10.3109/10520299509107311.

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12

Murray, G. I., M. D. Burke, and S. W. Ewen. "Enzyme histochemistry on freeze-dried, resin-embedded tissue." Journal of Histochemistry & Cytochemistry 37, no. 5 (1989): 643–52. http://dx.doi.org/10.1177/37.5.2539409.

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We have developed a method for histochemical demonstration of a wide range of enzymes in freeze-dried, resin-embedded tissue. Freeze-dried tissue specimens were embedded without fixation at low temperature (4 degrees C or -20 degrees C) in glycol methacrylate resin or LR Gold resin. Enzyme activity was optimally preserved by embedding the freeze-dried tissue in glycol methacrylate resin. All enzymes studied (oxidoreductases, esterases, peptidases, and phosphatases), except for glucose-6-phosphatase, were readily demonstrated. The enzymes displayed high activity and were accurately localized wi

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13

Yan, Dan, Renbin Li, Wei Lu, et al. "Flexible construction of cellulose photonic crystal optical sensing nano-materials detecting organic solvents." Analyst 144, no. 6 (2019): 1892–97. http://dx.doi.org/10.1039/c8an01236a.

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We developed a simple and efficient method to construct 3D and 2D opal and inverse opal cellulose photonic crystal films (CPCF) by embedding 3D or 2D polymethyl methacrylate (PMMA) colloidal arrays into carboxymethyl cellulose (CMC), respectively.

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14

Bruhl, J. J., and A. E. Ashford. "Application of Glycol Methacrylate Embedding to Herbarium Specimens of Fruits." Stain Technology 61, no. 3 (1986): 163–67. http://dx.doi.org/10.3109/10520298609110727.

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15

Nageris, Benny, and Dan Gazit. "Method for Embedding Temporal Bones of Rats in Methyl-Methacrylate." Annals of Otology, Rhinology & Laryngology 104, no. 10 (1995): 783–85. http://dx.doi.org/10.1177/000348949510401006.

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The commonly used method of preparing the temporal bone for light microscopy is a refinement of a basic formula that has been employed for a century. This process includes fixation, decalcification, neutralization, dehydration, embedding in celloidin, and hardening. The main disadvantage of this process is that decalcification is performed. This article describes a new method for preparing the temporal bone of rats for light microscopy. The main advantage of this new method is that no decalcification is involved, so that all bony elements are retained in their normal shape and location, and ev

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16

Brumback, R. A., D. L. Feeback, R. W. Leech, J. L. Ketring, and J. J. Davis. "GLYCOL METHACRYLATE EMBEDDING AND STAIN MODIFICATIONS FOR BRAIN TISSUE SPECIMENS." Journal of Neuropathology and Experimental Neurology 48, no. 3 (1989): 312. http://dx.doi.org/10.1097/00005072-198905000-00040.

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17

Gerrits, Peter O., and Albert J. H. Suurmeijer. "Glycol Methacrylate Embedding in Diagnostic Pathology:A Standardized Method for Processing and Embedding Human Tissue Biopsy Specimens." American Journal of Clinical Pathology 95, no. 2 (1991): 150–56. http://dx.doi.org/10.1093/ajcp/95.2.150.

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18

Murray, G. I., and S. W. Ewen. "Enzyme histochemistry on freeze-substituted glycol methacrylate-embedded tissue." Journal of Histochemistry & Cytochemistry 38, no. 1 (1990): 95–101. http://dx.doi.org/10.1177/38.1.2294150.

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We developed a method for histochemical demonstration of a wide range of enzymes in freeze-substituted glycol methacrylate-embedded tissue. Tissue specimens were freeze-substituted in acetone and then embedded at low temperature in glycol methacrylate resin. All enzymes studied (oxidoreductases, hydrolases) were readily demonstrated. The enzymes displayed high activity and were accurately localized without diffusion when tissue sections were incubated in aqueous media, addition of colloid stabilizers to the incubating media not being required. Freeze-substitution combined with low-temperature

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19

Prado, Cibele M., Fernanda Viaro, Caroline F. Baldo, Viviane dos Santos Augusto, Alfredo José Rodrigues, and Paulo Roberto Barbosa Evora. "Glycol methacrylate-embedding medium to study morphological alterations of saphenous vein under brief and crescent pressurizations." Acta Cirurgica Brasileira 23, suppl 1 (2008): 77–82. http://dx.doi.org/10.1590/s0102-86502008000700014.

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PURPOSE: This study sought to evaluate the efficiency of glycol methacrylate-embedding medium to detect morphological alterations of human saphenous vein submitted to brief and crescent pressurizations. METHODS: Saphenous veins of 20 CABG patients were randomly distributed into four experimental groups (control, 100, 200 and 300 mmHg pressures during 15 seconds). To quantify the percentage of endothelium spread over vein surface a microscope magnification of 100x was used for measurements. Morphometric analysis was performed using videomicroscopy with the Leica Qwin software in conjunction wit

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20

Murray, G. I., M. D. Burke, and S. W. Ewen. "Enzyme histochemical demonstration of NADH dehydrogenase on resin-embedded tissue." Journal of Histochemistry & Cytochemistry 36, no. 7 (1988): 815–19. http://dx.doi.org/10.1177/36.7.3385192.

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We describe a method for enzyme histochemical demonstration of NADH dehydrogenase in cold (4 degrees C)-processed resin-embedded tissue. The effects on NADH dehydrogenase activity of processing tissue through a variety of dehydrating agents and embedding in three different acrylic resins were evaluated. The optimal procedure to maintain NADH dehydrogenase activity used a short (3-hr) fixation in 1% paraformaldehyde solution, followed by dehydration in acetone and embedding in glycol methacrylate resin. Embedding of tissue in resin combined preservation and accurate localization of NADH dehydro

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21

BURGIO, V. L., P. PIGNOLONI, and C. D. BARONI. "Immunohistology of bone marrow: a modified method of glycol-methacrylate embedding." Histopathology 18, no. 1 (1991): 37–43. http://dx.doi.org/10.1111/j.1365-2559.1991.tb00812.x.

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22

Sanderson, Cathy A. "Extraction and Re-embedding Technique for Salvaging Impaired Glycol Methacrylate Specimens." Journal of Histotechnology 12, no. 1 (1989): 53–54. http://dx.doi.org/10.1179/his.1989.12.1.53.

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23

Gerrits, Peter O., and Richard W. Horobin. "Glycol Methacrylate Embedding for Light Microscopy: Basic Principles and Trouble-Shooting." Journal of Histotechnology 19, no. 4 (1996): 297–311. http://dx.doi.org/10.1179/his.1996.19.4.297.

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24

Lewis, G. H. J., and I. D. Bowen. "A methacrylate embedding technique for combined autoradiography and acid phosphatase histochemistry." Histochemical Journal 17, no. 4 (1985): 467–75. http://dx.doi.org/10.1007/bf01003206.

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25

Ring, P. K., and V. Johanson. "Immunoelectron microscopic demonstration of thyroglobulin and thyroid hormones in rat thyroid gland." Journal of Histochemistry & Cytochemistry 35, no. 10 (1987): 1095–104. http://dx.doi.org/10.1177/35.10.2442241.

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We have developed a post-embedding immunogold technique for electron microscopic localization and quantitation of thyroglobulin (TG), thyroxine (T4), and triiodothyronine (T3) in rat thyroid. Labeling for TG was located on rough endoplasmic reticulum, Golgi apparatus, exocytotic vesicles, luminal colloid, colloid droplets, and lysosomes, whereas labeling for thyroid hormones was located on luminal colloid, colloid droplets, and lysosomes. We tested different procedures of fixation, dehydration, embedding, polymerization, and immunoincubation to optimize ultrastructural preservation and immunol

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26

Blythe, D., N. M. Hand, P. Jackson, S. L. Barrans, R. D. Bradbury, and A. S. Jack. "Use of methyl methacrylate resin for embedding bone marrow trephine biopsy specimens." Journal of Clinical Pathology 50, no. 1 (1997): 45–49. http://dx.doi.org/10.1136/jcp.50.1.45.

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27

Chappard, Daniel, Sabine Palle, Christian Alexandre, Laurence Vico, and Georges Riffat. "Bone embedding in pure methyl methacrylate at low temperature preserves enzyme activities." Acta Histochemica 81, no. 2 (1987): 183–90. http://dx.doi.org/10.1016/s0065-1281(87)80012-0.

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28

Mongwaketsi, Nametso, Nolubabalo Matinise, Jack Madito, et al. "Silver nanostructures embedding in polymethyl methacrylate patterns fabricated using proton beam writing." Surface and Interface Analysis 52, no. 12 (2020): 1034–39. http://dx.doi.org/10.1002/sia.6758.

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29

Palle, Sabine, Daniel Chappard, Christian Alexandre, and Jean-Baptiste Orsini. "Friable rock embedding in methyl methacrylate for thin-sectioning in geological preparations." Journal of Microscopy 141, no. 2 (1986): 193–98. http://dx.doi.org/10.1111/j.1365-2818.1986.tb02715.x.

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30

Harris, P. J., and B. P. Rubin. "Transition from mitosis to interphase in sea urchin first division: immunofluorescence studies of tubulin distribution in methacrylate sections." Journal of Histochemistry & Cytochemistry 35, no. 3 (1987): 343–49. http://dx.doi.org/10.1177/35.3.3546483.

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Previous immunofluorescence studies of microtubule distribution in fertilized sea urchin eggs have suffered from poor resolution caused by cell thickness, unavoidable artifacts resulting from excessive flattening, or extraction by detergents of membranes and other lipid-containing structures that may be of interest in relation to the microtubules. To avoid these difficulties, we have developed a fixation and embedding protocol based on buffered paraformaldehyde fixation and butyl-methyl methacrylate embedment, which allows immunofluorescence staining of 0.5-1 micron sections. Polymerization ar

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31

Escolar, G., J. J. Sauk, M. L. Bravo, M. Krumwiede, and J. G. White. "Development of a simple embedding procedure allowing immunocytochemical localization at the ultrastructural level." Journal of Histochemistry & Cytochemistry 36, no. 12 (1988): 1579–82. http://dx.doi.org/10.1177/36.12.3192938.

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Immunobed solution A is a water-soluble acrylic compound recently developed for immunocytochemical localization at the light microscopic level. In this study, we combined it with methyl methacrylate (MMA) to achieve sufficient hardness to obtain ultra-thin sections. Samples of platelets were dehydrated and embedded in the water-soluble acrylic mixture (WSAM). The embedding process was carried out at 4 degrees C and final polymerization was induced with either chemical (benzoyl peroxide) or physical (UV light) catalysts. Tubulin was localized at the ultrastructural level in sections embedded ac

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32

van Goor, H., G. Harms, P. O. Gerrits, F. G. Kroese, S. Poppema, and J. Grond. "Immunohistochemical antigen demonstration in plastic-embedded lymphoid tissue." Journal of Histochemistry & Cytochemistry 36, no. 1 (1988): 115–20. http://dx.doi.org/10.1177/36.1.3275710.

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We describe a method for post-embedding immunohistochemical demonstration of a wide range of antigens in glycol methacrylate-embedded tissue. Rat spleen and thymus tissues were fixed by immersion in fixatives containing different concentrations of paraformaldehyde, washed in sucrose phosphate buffer, dehydrated in acetone, infiltrated in a glycol methacrylate mixture in which the commonly used softener 2-butoxyethanol was replaced by butaandiol monoacrylate, and embedded. Trypsin was used to re-expose the masked antigenicity. Excellent results were obtained with a panel of monoclonal antibodie

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33

Cruz, AL, MN Fernandes, and SF Perry. "Effect of histological processing and methacrylate sectioning on the area of gill tissue in teleost." Brazilian Journal of Biology 69, no. 2 (2009): 385–87. http://dx.doi.org/10.1590/s1519-69842009000200022.

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Deformation of biological tissues may occur during histological processing and results in loss of accuracy when quantitative information about cells, tissues and organs is necessary. In this study, the gill tissue from armored catfish (Pterygoplichthys anisitsi) was quantified in each step of processing using the stereological principles. During processing for glycol methacrylate embedding, gill tissue from shrinks significantly but regains its original dimensions after sectioning.

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34

Hwang, Sung-Ho, Seong Hui Hong, Young Kwang Kim, Won Wook Jeong, Oh Kyung Kwon, and Sang Kyoo Lim. "Preparation of hydrophobic fabric using cross-linked fluorinated poly methacryalte copolymer nanobeads." Textile Research Journal 89, no. 11 (2018): 2057–66. http://dx.doi.org/10.1177/0040517518794346.

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In this study, we synthesized cross-linked poly methyl methacrylate beads and cross-linked fluorinated poly methacryalte copolymer (F-cPMMA) beads by the dispersion polymerization method with methyl methacrylate monomer, ethylene glycol dimethacrylate, and 2,2,2-trifuloroethyl methacrylate. The size of cross-linked PMMA and F-cPMMA beads was about 100 nm. Then we developed a new geometrical high-hydrophobic film by embedding the F-cPMMA nanobeads on polyurethane (PU) film. The PU film containing fluorinated poly methacryalte copolymer beads showed a greater hydrophobic property than the non-fl

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35

Wide, M., H. Persson, S. Gunnarsson, L. Wide, and L. Seifi. "High frequency of in situ hybridization on thin plastic sections of human placenta with a cDNA probe for beta hCG." Journal of Histochemistry & Cytochemistry 37, no. 8 (1989): 1193–96. http://dx.doi.org/10.1177/37.8.2474022.

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We describe two different techniques with plastic embedding in in situ hybridization histochemistry (ISHH). Their applicability was demonstrated by use of human placenta of the tenth gestational week and a tritium-labeled cDNA probe for the beta-subunit of hCG. In the first method, ISHH was performed on whole pieces of tissue (en bloc ISHH) pretreated with a weak acid solution, embedded in methacrylate, and sectioned at 3 microns for autoradiography. In the second technique, en bloc ISHH was carried out on tissue pre-treated with the weak acid and thereafter with detergent to further facilitat

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36

Brumback, Roger A., Daniel L. Feeback, Richard W. Leech, Judy L. Ketring, and Julie J. Davis. "Modification of Brain Processing Techniques: Tissue Embedding in Glycol Methacrylate and Stain Modifications." Journal of Histotechnology 12, no. 3 (1989): 201–7. http://dx.doi.org/10.1179/his.1989.12.3.201.

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37

Gerrits, P. O., R. W. Horobin, and M. J. Hardonk. "A numerical procedure for choosing effective, low toxicity plasticizers for glycol methacrylate embedding." Histochemical Journal 22, no. 8 (1990): 439–51. http://dx.doi.org/10.1007/bf01003464.

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38

Gerrits, P. O., and M. B. M. van Leeuwen. "Glycol Methacrylate Embedding in Histotechnology: The Hematoxylin-Eosin Stain as A Method for Assessing the Stability of Glycol Methacrylate Sections." Stain Technology 62, no. 3 (1987): 181–90. http://dx.doi.org/10.3109/10520298709107990.

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39

Hermanns, W., F. Colbatzky, A. Günther, and B. Steiniger. "Ia antigens in plastic-embedded tissues: a post-embedding immunohistochemical study." Journal of Histochemistry & Cytochemistry 34, no. 6 (1986): 827–31. http://dx.doi.org/10.1177/34.6.3517152.

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The aim of the present study was to establish a plastic embedding technique that makes possible the immunohistochemical demonstration of class II major histocompatibility complex (MHC) antigens (Ia antigens) in undecalcified joint tissues. Therefore a series of fixatives and dehydrating agents was tested for saving Ia immunoreactivity by post-embedding immunostaining of thin sections (2 microns) of rat tissues that had been embedded in glycol methacrylate (GMA), and by comparing with cryostat sections. An indirect immunoperoxidase and the avidin-biotin complex (ABC) technique were used. Combin

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40

Maglio, M., F. Salamanna, S. Brogini, et al. "Histological, Histomorphometrical, and Biomechanical Studies of Bone-Implanted Medical Devices: Hard Resin Embedding." BioMed Research International 2020 (January 17, 2020): 1–13. http://dx.doi.org/10.1155/2020/1804630.

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The growing incidence of degenerative musculoskeletal disorders as well as lifestyle changes has led to an increase in the surgical procedures involving implanted medical devices in orthopedics. When studying implant/tissue interface in hard materials (i.e., metals or dense plastics) and/or in large bone segments, the hard plastic embedding of the intact undecalcified tissue envelope with the implant in situ is needed. The aim of this work is to describe the advances and the possibilities of high-temperature methyl methacrylate (MMA) embedding for the histological, histomorphometrical, and bio

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Zafar, Muhammad Sohail. "Prosthodontic Applications of Polymethyl Methacrylate (PMMA): An Update." Polymers 12, no. 10 (2020): 2299. http://dx.doi.org/10.3390/polym12102299.

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A wide range of polymers are commonly used for various applications in prosthodontics. Polymethyl methacrylate (PMMA) is commonly used for prosthetic dental applications, including the fabrication of artificial teeth, denture bases, dentures, obturators, orthodontic retainers, temporary or provisional crowns, and for the repair of dental prostheses. Additional dental applications of PMMA include occlusal splints, printed or milled casts, dies for treatment planning, and the embedding of tooth specimens for research purposes. The unique properties of PMMA, such as its low density, aesthetics, c

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Caldwell, Rebecca A., Linda Jenkins, Stephen D. Ainsworth, Eugene M. Langan, Timothy M. sullivan, and Martine LaBerge. "Evaluation of Endovascular Stents: Three Staining Procedures for Vascular Tissue Using Polymethyl Methacrylate Embedding." Journal of Histotechnology 25, no. 2 (2002): 81–85. http://dx.doi.org/10.1179/his.2002.25.2.81.

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Feeback, Daniel L., Judy L. Ketring-Hanna, Richard W. Leech, Lisa K. Benningfield, and Roger A. Brumback. "Methyl Methacrylate Embedding of Large Nervous Tissue Blocks for Neurohistologic, Immunocytochemical, and Ultrastructural Studies." Journal of Histotechnology 14, no. 2 (1991): 89–95. http://dx.doi.org/10.1179/his.1991.14.2.89.

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44

Park, Carol M., Philip E. Reid, David C. Walker, and Brian R. MacPherson. "A simple, practical ‘swiss roll’ method of preparing tissues for paraffin or methacrylate embedding." Journal of Microscopy 145, no. 1 (1987): 115–20. http://dx.doi.org/10.1111/j.1365-2818.1987.tb01321.x.

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45

Jiang, Guojun, and Sheng Xie. "Comparison of AFM Nanoindentation and Gold Nanoparticle Embedding Techniques for Measuring the Properties of Polymer Thin Films." Polymers 11, no. 4 (2019): 617. http://dx.doi.org/10.3390/polym11040617.

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The surfaces of polymer and interfaces between polymer and inorganic particles are of particular importance for the properties of polymers and composites. However, the determination of the properties of surfaces and interfaces poses many challenges due to their extremely small dimensions. Herein, polystyrene and polymethyl methacrylate thin film on silicon wafer was used as a model system for the measurement of the properties of the polymer near free surface and at the polymer-solid interface. Two different methods, i.e., nanoindentation using atomic force microscopy (AFM) and the gold nanopar

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Pretlow, Theresa P., and Thomas G. Pretlow. "Premalignant Changes Identified by Enzyme-Histochemistry with Methacrylate Sections." Microscopy and Microanalysis 3, S2 (1997): 1–2. http://dx.doi.org/10.1017/s1431927600006899.

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The development of cancer is characterized by a long latency between the initial exposure to a carcinogenic substance and the final appearance of a macroscopic tumor. Histochemistry has provided the means to identify specific cells involved in this process and many of the changes that take place during the transformation of normal cells to malignant ones. Putative premalignant lesions have been studied extensively in liver carcinogenesis. Histological sections of grossly normal appearing liver a few weeks after treating animals with carcinogen contain discrete areas or “islands” of aberrant en

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47

Yang, Bo, Dawei Pan, Laixi Sun, Shufan Chen, Weidong Wu, and Bo Li. "Fabrication of Polymer Composite Fibers Embedding Ultra-Long Micro/Nanowires." Nanomaterials 11, no. 4 (2021): 939. http://dx.doi.org/10.3390/nano11040939.

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Fabrication of polymer composite fibers embedding ultra-long micro/nanowires via an iterative melt co-drawing and bundling technique is reported in this study. The poly(methyl methacrylate) (PMMA) porous array templates were prepared with section-cutting the PMMA/polystyrene (PS) (shell/core) composite fibers and dissolution of inner PS. The results showed that the PS cores or pores in the PMMA matrix are regularly arranged with hexagonal, and their diameter and spacing exhibits a uniform distribution. Especially, the core diameter can be precisely controlled from millimeter-scale to nanometer

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Tobler, M., B. Wüthrich, and A. U. Freiburghaus. "Contact dermatitis from acrylate and methacrylate compounds in Lowicryl® embedding media for electron microscopy." Contact Dermatitis 23, no. 2 (1990): 96–102. http://dx.doi.org/10.1111/j.1600-0536.1990.tb03230.x.

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Occhetta, Paola, Roberta Visone, Laura Russo, Laura Cipolla, Matteo Moretti, and Marco Rasponi. "VA-086 methacrylate gelatine photopolymerizable hydrogels: A parametric study for highly biocompatible 3D cell embedding." Journal of Biomedical Materials Research Part A 103, no. 6 (2014): 2109–17. http://dx.doi.org/10.1002/jbm.a.35346.

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Alves, A., and M. Therin. "New Glycol Methacrylate Embedding Procedure for Enzyme and Immunohistochemical Assessment of Tissue Response to Biomaterials." Journal of Histotechnology 20, no. 1 (1997): 53–59. http://dx.doi.org/10.1179/his.1997.20.1.53.

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