Dissertations / Theses on the topic 'Modulatory signals'

Acute myeloid leukemia (AML) is a life-threatening malignant disorder with dismal prognosis. AML is characterized by frequent genetic changes involving tyrosine kinases, normally acting as important mediators in many basic cellular processes. Due to the overexpression and frequent mutations of the FMS-like receptor tyrosine kinase 3 (FLT3) in AML, this tyrosine kinase receptor has become one of the most sought after targets in AML drug development. In this thesis, we have used a combination of high-throughput screens, direct target interaction assays and sequential cellular screens, including primary patient samples, as an approach to discover new targeted therapies. Gefitinib, a previously known inhibitor of epidermal growth factor receptor and the two novel tyrosine kinase inhibitors AKN-032 and AKN-028, have been identified as compounds with cytotoxic activity in AML. AKN-028 is a potent inhibitor of FLT3 with an IC50 value of 6 nM in an enzyme assay, but also displaying in vitro activity in a variety of primary AML samples, irrespective of FLT3 mutation status or quantitative FLT3 expression. AKN-028 shows a sequence dependent in vitro synergy when combined with standard cytotoxic agents cytarabine or daunorubicin, with better efficacy when cells are exposed to standard chemotherapy simultaneously or for 24 hours prior to adding AKN-028. Antagonism is observed when cells are pre-treated with AKN-028, possibly explained by the cell cycle arrest induced by the compound. In vivo cytotoxic activity and good oral bioavailability have made AKN-028 a candidate drug for clinical studies and the compound is presently investigated in an international two-part multicenter phase I/II study. Results from microarray studies performed to further elucidate the mechanism of action of AKN-028, revealed significantly altered gene expression induced by AKN-028 in both AML cell lines and in primary AML cells, with an enrichment of the Myc pathway among the downregulated genes. Furthermore, tyrosine kinase activity profiling shows a dose-dependent kinase inhibition by AKN-028 in all AML samples tested. Interestingly, cells with a high overall kinase activity were more sensitive to AKN-028. Provided conformation in a larger set of samples, kinase activity profiling may give useful information in individualizing treatment of patients with AML.

This thesis reports original work on digital compensation for frequency dependent transfer characteristics and errors in digital PAM/CPFSK (Pulse Amplitude Modulation/Continuous Phase Frequency Shift Keying) quadrature modulators.A particularly flexible and cost effective approach to the implementation of the base-band section of a radio transmitter is to synthesise base-band signals digitally using a DSP (Digital Signal Processor). This approach is limited, however, by the transfer characteristics and errors in the implementation of practical analogue sub-systems. These practical limitations result in undesirable in-band frequency-dependent shaping of the transmitted signals. In the case of FM (Frequency Modulation) signals, this leads to the generation of unwanted side-lobes in the transmitted RF signal spectrum that interfere with signals in adjacent frequency channels. This results in the transmitted signal failing to meet transmission standards requirements.The digital compensation techniques developed and presented in this thesis allow the reduction of undesirable in-band frequency-dependent signal shaping. It is shown that this enables strict requirements on the spectral emissions from the FM transmitter to be met using a flexible and cost effective DSP based modulator system.The contributions of the thesis are in three primary areas:(i) The development of a structure for frequency dependent digital compensation.(ii) The formulation and solution of an optimisation problem that allows the free parameters within the structure to be determined such that effective reduction of unwanted in-band frequency-dependent signal shaping is achieved.(iii) The development of techniques that allow the digital compensation procedure to be successfully implemented on a practical DSP platform.The new digital compensation structure that is proposed uses two digital FIR (Finite ++<br>Impulse Response) filters in the digital base-band section of the quadrature modulator: one in the in-phase (I) channel and one in the quadrature (Q) channel. The tap-weights of the FIR filters are determined by solving two optimisation problems: one for each channel. The optimisation problems are formulated using a new approach that ensures that the degrees of freedom in the optimisation i.e., the FIR filter tap-weights, are used effectively to meet the objective of reducing in-band frequency-dependent signal shaping in analogue sub-systems further down the transmission path.A characterisation of the solutions to the optimisation problems enables the identification of techniques that need to be adopted to successfully implement the proposed digital compensation on a practical DSP-based system.The digital compensation technique is demonstrated by implementing and testing the technique on a DSP platform. The results of experimental studies are presented which clearly demonstrate that the digital compensation technique leads to substantial reductions in adjacent channel interference.

The continuous conduction mode (CCM) boost, buck-boost and buck-boost derived pulse-width modulation dc-dc converters suffer from the large-signal control-to-output nonlinearity. Without feedback control, the large-signal control-to-output nonlinearity would lead to output overregulation and even damage the components. The control gain is defined as the ratio of output voltage to control signal. The small-signal control gain is defined as differentiating output voltage with respect to control signal. Feedback control helps to make the output trace the reference signal. A large-signal control-to-output linearity is established. Compared with open loop control, the feedback loop design is complex; and the feedback control might suffer from the instability caused by the negative small-signal control gain, which is due to the loss and parasitic in practice. Except feedback control, open loop linearization methods can also realize the large-signal control-to-output linearity. A modulatedâ ramp pulse-width modulation generator is introduced in [6]. A current source works as the control signal. A capacitor is charged by the current source, whose voltage works as the carrier and compared with a constant dc bias voltage to determine the duty cycle. When applying this method to boost, buck-boost and buck-boost derived PWM dc-dc converters, a large-signal control-to-output linearity is established. However, the control gain is dependent on the input voltage; it cannot maintain constant when input voltage varies. A feedforward pulse width modulator is introduced in [39] to realize a large-signal control-to-output linearity. The static conversion ratio is divided into numerator and denominator as the functions of duty cycle. An integrator with reset clock signal helps to determine the right timing. The control gain is ideally constant and independent of input voltage. However, the mismatch between the integrator time constant and the switching period would result in a nonlinear control gain, which is dependent on the input voltage. In the thesis work, a self-oscillating unified linearizing modulator is introduced. It first provides a unified procedure to establish a large-signal control-to-output linearity for different pulse-width modulation dc-dc converters. Feedforward is employed to mitigate the impact from line voltage. Self-oscillation is adopted to provide the internal clock signal and to determine the switching frequency. A constant control gain is obtained, independent on the input voltage or the mismatch between clock signals. The modulator is constructed by three simple and standard building blocks. With the considerations of parasitic components and loss, how to design the constant gain, which excludes the negative small-signal control gain within the entire control signal range, is analyzed and discussed. The performance of this self-oscillating unified linearizing modulator is verified by experiments. The impacts from propagation delay in practical components are taken into considerations, which improves the quality of generated signals. Combined with a boost converter, a good large-signal control-to-output linearization is demonstrated. In the future work, the small-signal control-to-output transfer function is first deduced based on the SOUL modulator. Bode plots show the unique characteristic based on the SOUL modulator compared with the conventional modulator. Next, the impacts from this unique characteristic to feedback loop design and dynamic performance are discussed.<br>Master of Science

Recently short word-length (normally 1 bit or bits) processing has become a promising technique. However, there are unresolved issues in sigma-delta modulation, which is the basis for 1b/2b systems. These issues hindered the full adoption of single-bit techniues in industry. Among these problems is the stability of high-order modulators and the limit cycle behaviour. More importantly, there is no adaptive LMS structure of any kind in 1b/2b domain. The challenge in this problem is the harsh quantization that prevents straightforward LMS application. In this thesis, the focus has been made on three axes: designing new single-bit DSP applications, proposing novel approaches for stability analysis, and tacking the unresolved problems of 1b/2b adaptive filtering. Two structures for 1b digital comb filtering are proposed. A ternary DC blocker structure is also presented and performance is tested. We also proposed a single-bit multiplierless DC-blocking structure. The s tability of a single-bit high-order signma-delta modulator is studied under dc inputs. A new approach for stability analysis is proposed based on analogy with PLL analysis. Finally we succeeded in designing 1b/2b Wiener-like filtering and introduced (for the first time) three 1b/2b adaptive schemes.

Coherent optical data processing is recognised, for many applications, as a viable alternative to digital electronic signal processing; the case for using coherent optics is particularly strong when the data to be processed is two dimensional in nature. It has long been accpeted that, in order for coherent optical processing to achieve its full performance potential, two dimensional spatial light modulators - capable of operating in real time - are essential at both the object plane (where the data is input to the system) and the Fourier plane (where the operation carried out on the data is determined). Most previous research in the field of spatial modulators has concentrated on optically addressed devices for use in the object plane. This thesis describes a prototype liquid crystal over silicon spatial light modulator built to test the feasibility of using such devices in a coherent optical processor. Optically, the device operates as a binary amplitude modulator, consisting of a square array of 16x16 pixels, each of size 100x100 m^2 and located at 200m centres. The integrated circuit is designed for a 6m wafer fabrication process. Each pixel of the IC contains a static memory element (which stores a digital logic voltage corresponding to the optical state of that pixel) and provides a stable square wave voltage signal to drive the liquid crystal layer. The component parts of the spatial light modulator are tested individually: the liquid crystal, in test cells, for contrast and switching speed; the IC for electrical performance and optical (flatness) characteristics. The effect of pixellation on optical performance is investigated. The performance of live devices is demonstrated. The results indicate the feasibility of using such a device as a binary amplitude spatial light modulator.

Les communications optiques basées sur la photonique sur silicium (SiP) sont au centre des récents efforts de recherche pour le développement des futures technologies de réseaux optiques à haut débit. Dans cette thèse, nous étudions le traitement numérique du signal (DSP) pour pallier aux limites physiques des modulateurs Mach-Zehnder sur silicium (MZM) opérés à haut débit et exploitant des formats de modulation avancés utilisant la détection cohérente. Dans le premier chapitre, nous présentons une nouvelle méthode de précompensation adaptative appelée contrôle d’apprentissage itératif par gain (G-ILC, aussi utilisé en linéarisation d’amplificateurs RF) permettant de compenser les distorsions non-linéaires. L’adaptation de la méthode G-ILC et la précompensation numérique linéaire sont accomplies par une procédure « hardware-in-the-loop » en quasi-temps réel. Nous examinons différents ordres de modulation d’amplitude en quadrature (QAM) de 16QAM à 256QAM avec des taux de symboles de 20 à 60 Gbaud. De plus, nous combinons les précompensations numériques et optiques pour contrevenir surmonter les limitations de bande-passante du système en régime de transmission haut débit. Dans le second chapitre, inspiré par les faibles taux de symbole du G-ILC, nous augmentons la vitesse de transmission au-delà de la limite de bande-passante du système SiP. Pour la première fois, nous démontrons expérimentalement un record de 100 Gbaud par 16QAM et 32QAM en transmission consécutive avec polarisation mixte. L’optimisation est réalisée sur le point d’opération du MZM et sur la DSP. Les performances du G-ILC sont améliorées par égalisation linéaire à entrées/sorties multiples (MIMO). Nous combinons aussi notre précompensation non-linéaire innovante avec une post-compensation. Par émulation de la polarisation mixte, nous réalisons un taux net de 833 Gb/s avec 32QAM au seuil de correction d’erreur (FEC) pour une expansion en largeur de bande de 20% et 747 Gb/s avec 16QAM (une expansion en largeur de bande de 7% du FEC). Dans le troisième chapitre, nous démontrons expérimentalement un algorithme de précompensation numérique basé sur une table de consultation (LUT) unidimensionnelle pour compenser les non-linéarités introduites à l’émetteur, e.g. réponse en fréquence non-linéaire du MZM en silicium, conversion numérique-analogique et amplificateur RF. L’évaluation est réalisée sur un QAM d’ordre élevé, i.e. 128QAM et 256QAM. Nous examinons la diminution en complexité de la LUT et son impact sur la performance. Finalement, nous examinons la généralisation de la méthode de précompensation proposée pour des jeux de données différents des données d’apprentissage de la table de consultation.<br>Les communications optiques basées sur la photonique sur silicium (SiP) sont au centre des récents efforts de recherche pour le développement des futures technologies de réseaux optiques à haut débit. Dans cette thèse, nous étudions le traitement numérique du signal (DSP) pour pallier aux limites physiques des modulateurs Mach-Zehnder sur silicium (MZM) opérés à haut débit et exploitant des formats de modulation avancés utilisant la détection cohérente. Dans le premier chapitre, nous présentons une nouvelle méthode de précompensation adaptative appelée contrôle d’apprentissage itératif par gain (G-ILC, aussi utilisé en linéarisation d’amplificateurs RF) permettant de compenser les distorsions non-linéaires. L’adaptation de la méthode G-ILC et la précompensation numérique linéaire sont accomplies par une procédure « hardware-in-the-loop » en quasi-temps réel. Nous examinons différents ordres de modulation d’amplitude en quadrature (QAM) de 16QAM à 256QAM avec des taux de symboles de 20 à 60 Gbaud. De plus, nous combinons les précompensations numériques et optiques pour contrevenir surmonter les limitations de bande-passante du système en régime de transmission haut débit. Dans le second chapitre, inspiré par les faibles taux de symbole du G-ILC, nous augmentons la vitesse de transmission au-delà de la limite de bande-passante du système SiP. Pour la première fois, nous démontrons expérimentalement un record de 100 Gbaud par 16QAM et 32QAM en transmission consécutive avec polarisation mixte. L’optimisation est réalisée sur le point d’opération du MZM et sur la DSP. Les performances du G-ILC sont améliorées par égalisation linéaire à entrées/sorties multiples (MIMO). Nous combinons aussi notre précompensation non-linéaire innovante avec une post-compensation. Par émulation de la polarisation mixte, nous réalisons un taux net de 833 Gb/s avec 32QAM au seuil de correction d’erreur (FEC) pour une expansion en largeur de bande de 20% et 747 Gb/s avec 16QAM (une expansion en largeur de bande de 7% du FEC). Dans le troisième chapitre, nous démontrons expérimentalement un algorithme de précompensation numérique basé sur une table de consultation (LUT) unidimensionnelle pour compenser les non-linéarités introduites à l’émetteur, e.g. réponse en fréquence non-linéaire du MZM en silicium, conversion numérique-analogique et amplificateur RF. L’évaluation est réalisée sur un QAM d’ordre élevé, i.e. 128QAM et 256QAM. Nous examinons la diminution en complexité de la LUT et son impact sur la performance. Finalement, nous examinons la généralisation de la méthode de précompensation proposée pour des jeux de données différents des données d’apprentissage de la table de consultation.<br>Optical communications based on silicon photonics (SiP) have become a focus of the recent research for future high speed optical network technologies. In this thesis, we investigate digital signal processing (DSP) approaches to combat the physical limits of SiP Mach-Zehnder modulators (MZM) driven at high baud rates and exploiting advanced modulation formats with coherent detection. In the first section, we present a novel adaptive pre-compensation method known as gain based iterative learning control (G-ILC, previously used in RF amplifier linearization) to overcome nonlinear distortions. We experimentally evaluate the G-ILC technique. Adaptation of the G-ILC, in combination with linear digital pre-compensation, is accomplished with a quasireal- time hardware-in-the-loop procedure. We examine various orders of quadrature amplitude modulation (QAM), i.e., 16QAM to 256QAM, and symbol rates, i.e., 20 to 60 Gbaud. Furthermore, we exploit joint digital and optical linear pre-compensation to overcome the bandwidth limitation of the system in the higher baud rate regime. In the second section, inspired by lower symbol rate G-ILC results, we push the baud rate beyond the bandwidth limit of the SiP system. For the first time, we experimentally report record-breaking 16QAM and 32QAM at 100 Gbaud in dual polarization back-to-back transmission. The optimization is performed on both MZM operating point and DSP. The G-ILC performance is improved by employing linear multiple input multiple output (MIMO) equalization during the adaptation. We combine our innovative nonlinear pre-compensation with post-compensation as well. Via dual polarization emulation, we achieve a net rate of 833 Gb/s with 32QAM at the forward error correction (FEC) threshold for 20% overhead and 747 Gb/s with 16QAM (7% FEC overhead). In the third section, we experimentally present a digital pre-compensation algorithm based on a one-dimensional lookup table (LUT) to compensate the nonlinearity introduced at the transmitter, e.g., nonlinear frequency response of the SiP MZM, digital to analog converter and RF amplifier. The evaluation is performed on higher order QAM, i.e., 128QAM and 256QAM. We examine reduction of LUT complexity and its impact on performance. Finally, we examine the generalization of the proposed pre-compensation method to data sets other than the original training set for the LUT.<br>Optical communications based on silicon photonics (SiP) have become a focus of the recent research for future high speed optical network technologies. In this thesis, we investigate digital signal processing (DSP) approaches to combat the physical limits of SiP Mach-Zehnder modulators (MZM) driven at high baud rates and exploiting advanced modulation formats with coherent detection. In the first section, we present a novel adaptive pre-compensation method known as gain based iterative learning control (G-ILC, previously used in RF amplifier linearization) to overcome nonlinear distortions. We experimentally evaluate the G-ILC technique. Adaptation of the G-ILC, in combination with linear digital pre-compensation, is accomplished with a quasireal- time hardware-in-the-loop procedure. We examine various orders of quadrature amplitude modulation (QAM), i.e., 16QAM to 256QAM, and symbol rates, i.e., 20 to 60 Gbaud. Furthermore, we exploit joint digital and optical linear pre-compensation to overcome the bandwidth limitation of the system in the higher baud rate regime. In the second section, inspired by lower symbol rate G-ILC results, we push the baud rate beyond the bandwidth limit of the SiP system. For the first time, we experimentally report record-breaking 16QAM and 32QAM at 100 Gbaud in dual polarization back-to-back transmission. The optimization is performed on both MZM operating point and DSP. The G-ILC performance is improved by employing linear multiple input multiple output (MIMO) equalization during the adaptation. We combine our innovative nonlinear pre-compensation with post-compensation as well. Via dual polarization emulation, we achieve a net rate of 833 Gb/s with 32QAM at the forward error correction (FEC) threshold for 20% overhead and 747 Gb/s with 16QAM (7% FEC overhead). In the third section, we experimentally present a digital pre-compensation algorithm based on a one-dimensional lookup table (LUT) to compensate the nonlinearity introduced at the transmitter, e.g., nonlinear frequency response of the SiP MZM, digital to analog converter and RF amplifier. The evaluation is performed on higher order QAM, i.e., 128QAM and 256QAM. We examine reduction of LUT complexity and its impact on performance. Finally, we examine the generalization of the proposed pre-compensation method to data sets other than the original training set for the LUT.

[Formulae and special characters can only be approximated here. Please see the pdf version of the abstract for an accurate reproduction.] In In-phase/Quadrature (IQ) modulator generating Continuous-Phase-Frequency-Shift-Keying (CPFSK) signals, shortcomings in the implementation of the analogue reconstruction filters result in the loss of the constant envelope property of the output signal. Ripples in the envelope function cause undesirable spreading of the transmitted signal spectrum into adjacent channels when the signal passes through non-linear elements in the transmission path. This results in the failure of the transmitted signal in meeting transmission standards requirements. Therefore, digital techniques compensating for these shortcomings play an important role in enhancing the performance of the IQ modulator. In this thesis, several techniques to compensate for the irregularities in the I and Q channels are presented. The main emphasis is on preserving a constant magnitude and linear phase characteristics in the pass-band of the analogue filters as well as compensating for the imbalances between the I and Q channels. A generic digital pre-compensation model is used, and based on this model, the digital compensation schemes are formulated using control and signal processing techniques. Four digital compensation techniques are proposed and analysed. The first method is based on H2 norm minimization while the second method solves for the pre-compensation filters by posing the problem as one of H∞ optimisation. The third method stems from the well-known principle of Wiener filtering. Note that the digital compensation filters found using these methods are computed off-line. We then proceed by designing adaptive compensation filters that runs on-line and uses the “live” modulator input data to make the necessary measurements and compensations. These adaptive filters are computed based on the well-known Least-Mean-Square (LMS) algorithm. The advantage of using this approach is that the modulator does not require to be taken off-line in the process of calculating the pre-compensation filters and thus will not disrupt the normal operation of the modulator. The compensation performance of all methods is studied analytically using computer simulations and practical experiments. The results indicate that the proposed methods are effective and are able to provide substantial compensation for the shortcomings of the analogue reconstruction filters in the I and Q channels. In addition, the adaptive compensation scheme, implemented on a DSP platform shows that there is significant reduction in side-lobe levels for the compensated signal spectrum.

Processing of microwave signals in electrical domain introduces many difficulties especially when the frequency of the signal is increased beyond several GHz. Electromagnetic interference (EMI) and frequency depended losses can be given as examples to these difficulties. Photonic processing of microwave signals, however, is immune to these problems since optical components such as fiber cables, lasers, optical modulators and photodetectors are both immune to EMI and have wide bandwidths. This thesis deals with down conversion of a microwave signal using a Mach-Zender modulator and filtering unwanted harmonics using a photonic filter.

In this thesis work large signal model of cascaded travelling wave electroabsorption modulator (CTWEAM) has been studied. The effect of input modulating voltage, input optical power, biasing voltage, and circuit parameters on bandwidth, extinction ratio and overshooting of this modular carefully investigated. It has been also investigated the sensitivity of the transit time delay and the length ratio of active segment and microstrip line over the total length on the performance of the modulator. As the modulating voltage, input optical power and biasing voltage are increased then the bandwidth, extinction ratio and overshooting are also increased until certain value (except for modulating voltage) after that increasing of input optical power and biasing voltage the bandwidth, extinction ratio and overshooting are decreased. At 1.1 V biasing voltage, 14 mW input optical power and only 0.4 V(p-p) modulating voltage the cascading modulator has 3dB bandwidth, extinction ratio and overshooting 110 GHz, 4 dB and 0.2 dB respectively. It has been shown that at the same biasing voltage and input optical power but at 1 V(p-p) modulating voltage the cascading modulator has 119 GHz 3dB bandwidth, 10.9 dB extinction ratio and 0.47 dB overshooting that could be applicable for telecommunication. It has been seen that the microwave reflection loss is -10 dB at 100 GHz to perform the device on the above mentioned values. It has been shown that the cascading modulator to perform on the above mentioned values, the p-doped mesa structure has changed from rectangular to mushroom shape which has 0.69 Ω-mm, total active segment length is 990 μm where first, second, third and fourth active segment have 11%, 10%, 22% and 56% length over the total active segment length. Since the cascading modulator performance has been studied on large signal model, the results have been obtained from this thesis work would be the crucial for optimum designing and fabrication of the cascading modulator to apply in real life application.

The thesis focuses on consideration of possible ways how to realize multi-state modulators for a laboratory instrument. This instrument will illustrate signals, which partake on generation QAM modulation. The design of the laboratory instrument includes theoretic schemata and the execution of a prototype. There are theoretical facts of multi-state signals and multi-state modulations in this thesis. A simple block diagram of the laboratory instrument is made by the theory of creating quadrature amplitude modulation. For each block there are made several schemata, which are compiled to the global scheme. A printed circuit board and the structure items are made by the aid of the global scheme. The next part is devoted to a software facility for a used microprocessor, which ensures the whole function of the laboratory instrument. The final section deals with measuring, which checks the function of the modulator and the right setting coder’s constellation diagrams.

This project occupies with the creation modulated signal generator in MATLAB. There are explicit basic princips of modulation PSK, M-QAM, princip of system with spread spektrum and princip of access OFDM and MC-CDMA. Next there are explicit basic parametres and possibilities configuration of conversion analog PCI card CompuGen 4302, which serves as D/A convertor of signals created by PC.

In todays fast evolving mobile communications the requirements of higher datarates are continuously increasing, pushing operators to upgrade the backhaul to support these speeds. A cost eective way of doing this is by using microwave links between base stations, but as the requirements of data rates increase, the capacity of the microwave links must be increased. This thesis was part of a funded research project with the objective of developing the next generation high speed microwave links for the E-band. In the research project there was a need for a testing system that was able to generate a series of test signals with selectable QAM modulations and adjustable properties to be able to measure and evaluate hardware within the research project. The developed system was designed in a digital domain using an FPGA platform from Altera, and had the ability of selecting several types of modulations and changing the properties of the output signals as requested. By using simulation in several steps and measurements of the complete system the functionality was verified and the system was delivered to the research project successfully. The developed system can be used to test several dierent modulators in other projects as well and is easily extended to provide further properties.

The goal of the theses is to propose an idea for a generator of a radar polyphase modulated signal, that will operate at a frequency of 30 MHz. At the beginning of this project, an outline of the basic structure of the generator is proposed and a function of a programable logical perimeter is analyzed. This perimeter, together with an oscillator, generate two mutually phase-shifted rectangular pulses in its outputs. The shift phase of these two pulses is set by a program, and operated by single-chip processor. Those pulses are consequently converted to sinusoidal wave with the help of filters, and the required phase is chosen by the multiplexer, which is also operated by the processor. The phase modulation is realized this way. The output signal of the generator is radiopulse, which subpulses are phase modulated by the described method. Individual parts are solved analogously in this Theses.

A dual-mode second-order Multirate Multibit Sigma Delta (MM-SD) modulator is implemented in a 90nm digital CMOS process for application in the baseband path of RF receivers. Low power consumption is achieved through a new integrator structure and a dedicated timing scheme along with aggressive capacitor scaling in the second stage of the modulator loop. Fabricated prototype achieves 68.6dB peak Signal-to-Noise and Distortion ratio (SNDR) in the 200 kHz GSM band and requires 1.1mA of total current from a 1.5V supply. This dual-mode design also achieves 42.8dB SNDR in the 1.94 MHz WCDMA band with only 1.9mA of total current consumption.

According to the cancer stem cells (CSCs) hypothesis, CSCs are responsible for the treatment failures. CSCs are a subset of cells possessing stemness properties within the heterogeneous tumor mass. Therapeutic intervention on Wnt signaling is of our great interest because an aberrant Wnt signaling is an important driver to maintain the potency of CSCs. In nasopharyngeal carcinoma (NPC), deregulated expression of the Wnt signaling components is frequently observed. ICG-001 is a selective Wnt modulator (CBP antagonist) that specifically interrupts the interaction between β-catenin and CBP, thereby encourages the interaction between β-catenin and p300 and the subsequent differentiation and reduction of the CSCs subset. For this reason, the present study aimed to evaluate the therapeutic potential of ICG-001 in NPC. Results showed that ICG-001 inhibited both the migration of the NPC cells and the formation of tumor spheres. In the first part of the mechanistic studies (Chapter 3), ICG-001 was found to restore the expression of miR-150 in NPC cells. MiR-150 was further found to directly reduce CD44 expression and inhibit NPC cell migration. In the second part of the mechanistic studies (Chapter 4), ICG-001 was found to reduce the expression of Evi1 in NPC cells. The effect was accompanied with the inhibition of both the NPC cells migration and the tumor spheres formation. Two molecular axes, namely miR-96/Evi1/miR-449a and survivin/Evi1/miR-449a, were found to be involved in the inhibition of the tumor cell migration and spheroids formation. The therapeutic potential of using this CBP antagonist (ICG-001) in NPC, namely the in vitro and in vivo efficacy of ICG-001 combined with cisplatin, was examined (Chapter 5). Concurrent treatment of ICG-001 and cisplatin exhibited a synergistic inhibition on the in vitro growth and the tumor sphere forming capacity of NPC cells as well as the growth of NPC xenografts. Taken together, results presented in this thesis suggested that ICG-001 (PRI-724 is the analog of ICG-001 currently used in clinical trials) has a therapeutic potential in NPC.

This work deals with the transmission of optical radiation modulated by a radio frequency signal through an optical fiber. Furthermore, it describes the principles of communication and area coverage. It points out the practical use of components located in the communication chain of this system. Explains the functionality of the properties and the division of individual components. It introduces the benefits and limitations of this system. It further describes the creation of a topology suitable for laboratory measurement of RoF technology. Provides experimental measurement of C-band parameters. Indicates the possible use of RoF technology using a wireless optical link. A part of the work is also a sample laboratory protocol.

Master’s thesis deals with design and practical realisation of electronic circuits, which are needed for assembling of DRM signal transmitter for ham short waves bands. There is presented DRM standard as well as there are described the differencies between DRM for radio broadcast and for ham using. There is described design of input audio circuits, modulator, mixer, local generator, amplifier and filters. Principle of used SSB modulator is based on phase method, often called Tayloe modulator. This principle is analysed in detail including mathematical description, which was derived. It is possible to control the transmitter by program running at computer, communication takes place via USB. There is described establishment of communication in this work too.

<p>During the last decades, chemotherapy with cytotoxic drugs has played a significant role in cancer therapy. It’s important to develop new anticancer drugs, and drug sensitivity testing in vitro can be used to find the right diagnosis for the newly developed substances.</p><p>The aim of this study was to investigate the cytotoxic activity of the new signal transduction modulators bortezomib, gefitinib and PKC412. The well-established substances cisplatin, cytarabine, doxorubicin and vincristin were investigated for comparison.</p><p>The activity of the cytotoxic drugs was analysed in human tumor samples from patients with ovarian carcinoma (n=16) and lymphoma (n=15) by using the Fluorometric Microculture Cytotoxicity Assay (FMCA). The testing of cellular drug resistance by FMCA was accomplished successfully in 33 out of the 34 samples (97%).</p><p>The results of this study indicated that the activity of cytotoxic drugs in tumor cells obtained from patients with ovarian carcinoma and lymphoma may be detected by the FMCA. It also suggested that bortezomib and gefitinib could represent promising agents for treatment of ovarian carcinoma and that PKC412 might be of less use for patients with this diagnose.</p>

<p>Beroende på i vilken miljö man har tänkt att använda trådlös kommunikation behöver man hitta en modulationsteknik som passar under rådande förhållanden. I början på 1980-talet utvecklade den Amerikanska militären en modulationsteknik som kallas för Shaped BPSK (SBPSK) avsedd att tillämpas i kommunikationslänkar med satelliter. Vidareutveckling av SBPSK ledde sedan fram till en förbättrad variant kallad Shaped Offset QPSK (SOQPSK). På senare år har denna modulationsteknik börjat användas i civila tillämpningar och vidareutvecklats ytterligare för att ge den än bättre prestanda. År 2004 antogs SOQPSK som en modulationsteknik i den internationella flygplanskommunikationsstandarden, IRIG-106. Versionen av SOQPSK som antogs i IRIG-106 har flera bra egenskaper som t. ex. dess spektraltäthet. Detta gör denna typ av modulationsteknik lämpad för kommunikationslänkar med bl.a. flygplan, satelliter och rymdsonder (‘deep-space’).</p><p>Målet med examensarbetet har varit att implementera algoritmer för att skicka och ta emot SOQPSK-modulerade signaler. Dessa algoritmer skulle utvecklas i VHDL för att sedan syntetiseras och programmera en FPGA. Uppgiften har givits av Syncore Technologies AB i Linköping.</p><p>Arbetet har resulterat i fungerande implementationer både i mjukvara och hårdvara. Hårdvarulösningen är verifierad att klara bithastiheter upp till 30 Mbit/s. Teoretisk information om allmän modulering/demodulering och specifikt kring SOQPSK behandlas i rapporten. Uppbyggnaden av en teoretisk sändar- och mottagarmodell utformad för SOQPSK-kommunikation beskrivs också i rapporten för att ge en bättre helhetsbild av implementationen som utförts.</p><p>Arbetets syfte är att ligga till grund för Syncore AB som utvecklar en kom- munikationslänk med SOQPSK-kompatibilitet.</p>

This paper explores the noise shaping and noise producing qualities of Delta-Sigma Modulators (DSM) and Pulse-Width Modulators (PWM). DSM has long been dominant in the Delta Sigma Analog-to-Digital Converter (DSADC) as a noise-shaped quantizer and time discretizer, while PWM, with a similar self oscillating structure, has seen use in Class D Power Amplifiers, performing a similar function. It has been shown that the PWM in Class D Amplifiers outperforms the DSM [1], but could this advantage be used in DSADC use-cases? LTSpice simulation and printed circuit board implementation and test are used to present data on four variations of these modulators: The DSM, PWM, the out-of-loop discretized PWM (OOLDP), and the cascaded modulator. A generic form of an Nth order loop filter is presented, where three orders of this generic topology are analyzed in simulation for each modulator, and two orders are used in physical testing.

O foco desta tese é a descrição e validação de uma arquitetura de interface para processamento de sinais analógicos para SOC de sinais mistos. A abordagem proposta apresenta a possibilidade de cobertura de uma larga faixa de freqüências com performance praticamente constante associada a uma estrutura digital de programação. A premissa é usar uma célula analógica fixa e promover a configuração da aplicação no domínio digital, levando a uma arquitetura de interface de sinais mistos. O emprego de um bloco analógico fixo busca eliminar a perda inerente de performance decorrente da própria estrutura de programação em circuitos reconfiguráveis analógicos. A emprego da programação no domínio digital abre espaço para usos da vasta gama de ferramentas disponíveis para o projeto em alto nível de abstração, simulação e síntese automática para implementar a aplicação alvo com excelente predição do desempenho final. A abordagem proposta baseia-se no conceito de translação em freqüência (mixagem) do sinal de entrada seguida pela sua conversão para o domínio ΣΔ. A estrutura de processamento possibilita o emprego de um bloco analógico constante, e também, um processamento uniforme de sinais de entrada indo de DC até altas freqüências. A aplicação é configurada no domínio ΣΔ onde a performance pode ser predita de acordo com as especificações alvo. Objetivando a exploração do espaço de projeto foi desenvolvido o modelo de performance teórico e de simulação. Os modelos desenvolvidos auxiliam no também no projeto físico da interface proposta. Objetivando, tanto a validação dos modelos propostos, bem como o desenvolvimento de aplicações, foram construídos dois protótipos. São apresentados os usos da interface como um ADC paramétrico multi-banda e como um multiplicador e um somador de sinais analógicos. É proposta também uma arquitetura para uma interface analógica multi-canal. Os resultados experimentais empregados para a caracterização da interface proposta suportam as vantagens da mesma.<br>The focus of this thesis is to discuss the development and modeling of an interface architecture to be employed for interfacing analog signals in mixed-signal SOC. We claim that the approach that is going to be presented is able to achieve wide frequency range, and covers a large range of applications with constant performance, allied to digital configuration compatibility. Our primary assumptions are to use a fixed analog block and to promote application configurability in the digital domain, which leads to a mixed-signal interface. The use of a fixed analog block avoids the performance loss common to configurable analog blocks. The usage of configurability on the digital domain makes possible the use of all existing tools for high level design, simulation and synthesis to implement the target application, with very good performance prediction. The proposed approach utilizes the concept of frequency translation (mixing) of the input signal followed by its conversion to the ΣΔ domain, which makes possible the use of a fairly constant analog block, and also, a uniform treatment of input signal from DC to high frequencies. The programmability is performed in the ΣΔ digital domain where performance can be closely achieved according to application specification. The interface performance theoretical and simulation model are developed for design space exploration and for physical design support. Two prototypes are built and characterized to validate the proposed model and to implement some application examples. The usage of this interface as a multi-band parametric ADC and as a two channels analog multiplier and adder are shown. The multi-channel analog interface architecture is also presented. The characterization measurements support the main advantages of the approach proposed.

La modulazione a durata d'impulso (PWM) è utilizzata soprattutto perchè permette di ottenere alta efficenza energetica. In ambito accademico è stato proposto un modulatore PWM che sfrutta la tecnica di noise shaping, Sigma Delta, per avere elevata fedeltà. Il lavoro di questa tesi è stato l'implementazione su FPGA del modulatore Sigma DeltaDigitale utilizzato: quarto ordine, con quantizzatore a 4 bit e SNR in banda di 60 dB. Il dimensionamento è stato fatto determinando l'effetto che la lunghezza delle parole dei segnali ha sul rumore prodotto dal sistema. Questo studio è stato svolto con analisi euristiche ed algoritmi di ricerca implementati in ambiente MATLAB. Lo studio fatto è di carattere generale ed estendibile a generiche architetture Sigma Delta.

Thesis advisor: Marc A.T. Muskavitch<br>The Notch pathway is an evolutionarily conserved mechanism of intercellular signaling that plays a central role in the development of metazoans. Here I summarize two genetic screens that utilize a rough eye phenotype created by Delta overexpression in the Drosophila eye to identify modulators of Notch pathway signaling activity. Among the many "hits" obtained from both screens, I have mapped to the Not1 gene a single complementation group that exhibits strong genetic interactions with Notch pathway mutants. NOT1 is a component of the CCR4-NOT complex, a global regulator of gene expression that exerts its effects through a variety of mechanisms, including mRNA deadenylation and direct transcriptional repression. I have conducted a series of genetic and molecular experiments in an effort to obtain more insight into the relationship between the CCR4-NOT complex and the Notch pathway. Both Not1 EMS mutations and RNAi-mediated knockdown of NOT1 expression produce phenotypes that mimic those of Notch loss-of-function pathway mutants. Knockdown of NOT1 in the developing bristle organ disrupts Notch-mediated inhibition of neuronal specification, resulting in supernumerary neurons and aberrant sheath cell specification. Knockdown of NOT1 within the developing wing margin disrupts expression of the Notch target genes Cut and Wingless, as well as the Notch ligand Delta. Phenotypic rescue experiments imply that Not1 functions downstream of Notch signal activation and acts directly on Notch target gene expression. These results suggest that NOT1 is required for Notch signal transmission in certain developmental contexts and implicate the CCR4-NOT complex as a positive regulator of the Notch pathway<br>Thesis (PhD) — Boston College, 2009<br>Submitted to: Boston College. Graduate School of Arts and Sciences<br>Discipline: Biology

This thesis describes a design of a simulation of transmission of digital information via communication system and equalization of communication function. The layout of communication channel with multiway transmission is described in following part. Next part is about hardware modulator which generate modulated signal which is transmitted via communication channel and after is sampled by A/D convertion card to computer, where is equalizated and demodulated in Simulink. In the last part of this thesis, there is proposal of the laboratory task and its sample solution.

<p>The increasing data rates in digital television networks increase the demands on data capacity of the current transmission channels. Through new standards, the capacity of exisiting channels is increased with new methods of error correction coding and modulation.</p><p>This thesis presents the design and implementation of a modulator for transmission of digital terrestrial television according to the Chinese DTMB standard.</p><p>The system is written in VHDL and is intended for implementation on an FPGA.</p>

Silicon photonics as a one of the most promising photonic integration technologies has attracted many attentions in recent years. The major feature of this technology is its compatibility with complementary metal-oxide semiconductor (CMOS) processes which makes it possible to integrate optical and electronic devices in a same chip and reduce the cost significantly. Another reason of using silicon photonics is the high index contrast between the silicon core and silicon dioxide cladding which ensures the high density integration of photonic devices on a single chip. Monolithic integration with electronic and optical circuits makes silicon photonics technology suitable for numerous applications. One example is microwave photonics (MWP). MWP is an area that studies the interaction between microwave and optical signal for the generation, processing, control and distribution of microwave signals by means of photonics. Silicon photonics offers a reduction in footprint, losses, packaging cost and power dissipation in MWP systems. This research in this thesis is focused on the design and fabrication of the silicon photonic devices for MWP signal processing and generation. Four MWP systems based on silicon photonic devices are proposed and experimentally demonstrated. 1) A single pass-band frequency-tunable MWP filter based on phase-modulation to intensity-modulation conversion in an optically pumped silicon-on-insulator (SOI) microring resonator (MRR) is designed and experimentally demonstrated. In the proposed filter, a phase-modulated optical signal is filtered by the SOI MRR, to have one first-order sideband suppressed by the MRR notch. The phase-modulated optical signal is converted to an intensity-modulated single-sideband (SSB) signal and detected at a photodetector (PD). The entire operation is equivalent to a single pass-band filter. The frequency tunability is achieved by tuning the resonance wavelength of the MRR, which is realized by optically pumping the MRR. A single pass-band MWP filter with a tunable center frequency from 16 to 23 GHz is experimentally demonstrated. 2) A broadband optically tunable MWP phase shifter with a tunable phase shift using three cascaded SOI MRRs that are optically pumped is designed and experimentally demonstrated. A microwave signal to be phase shifted is applied to an optical single-sideband (OSSB) modulator to generate an optical carrier and an optical sideband. The phase shift is introduced to the optical carrier by placing the optical carrier within the bandwidth of one resonance of the three cascaded MRRs. The experimental results show that by optically pumping the cascaded MRRs, a broadband MWP phase shifter with a bandwidth of 7 GHz with a tunable phase shift covering the entire 360o phase shift range is achieved. 3) A multi tap MWP filter with positive and negative coefficients using a silicon ring resonator modulator (RRM) is proposed and experimentally demonstrated. The RRM is designed and fabricated to operate based on the carrier depletion effect. The positive and negative coefficients are obtained by using opposite slopes of the modulation transmission response of the RRM. Two filter responses with two and three taps are experimentally demonstrated, showing the proof-of-principle for frequencies up to 18 GHz. 4) An approach to generate microwave signal based on enhanced four wave mixing (FWM) in an active silicon waveguide (SiWG) is studied. This SiWG is designed and fabricated, and the use of the active SiWG for MWP frequency multiplication to generate a frequency-sextupled millimeter-wave signal is experimentally demonstrated. Thanks to a reverse-biased p-n junction across the SiWG, the conversion efficiency of the FWM is improved, which leads to the improvement of the microwave frequency multiplication efficiency.

[EN] The processing of radiofrequency signals using photonics means is a discipline that appeared almost at the same time as the laser and the optical fibre. Photonics offers the capability of managing broadband radiofrequency (RF) signals thanks to its low transmission attenuation, a variety of linear and non-linear phenomena and, recently, the potential to implement integrated photonic subsystems. These features open the door for the implementation of multiple functionalities including optical transportation, up and down frequency conversion, optical RF filtering, signal multiplexing, de-multiplexing, routing and switching, optical sampling, tone generation, delay control, beamforming and photonic generation of digital modulations, and even a combination of several of these functionalities. This thesis is focused on the application of vector processing in the optical domain to radiofrequency signals in two fields of application: optical beamforming, and photonic vector modulation and demodulation of digital quadrature amplitude modulations. The photonic vector control enables to adjust the amplitude and phase of the radiofrequency signals in the optical domain, which is the fundamental processing that is required in different applications such as beamforming networks for direct radiating array (DRA) antennas and multilevel quadrature modulation. The work described in this thesis include different techniques for implementing a photonic version of beamforming networks for direct radiating arrays (DRA) known as optical beamforming networks (OBFN), with the objectives of providing a precise control in terrestrial applications of broadband signals at very high frequencies above 40 GHz in communication antennas, optimizing the size and mass when compared with the electrical counterparts in space application, and presenting new photonic-based OBFN functionalities. Thus, two families of OBFNs are studied: fibre-based true time delay architectures and integrated networks. The first allow the control of broadband signals using dispersive optical fibres with wavelength division multiplexing techniques and advanced functionalities such as direction of arrival estimation in receiving architectures. In the second, passive OBFNs based on monolithically-integrated Optical Butler Matrices are studied, including an ultra-compact solution using optical heterodyne techniques in silicon-on-insulator (SOI) material, and an alternative implementing a homodyne counterpart in germanium doped silica material. In this thesis, the application of photonic vector processing to the generation of quadrature digital modulations has also been investigated. Multilevel modulations are based on encoding digital information in discrete states of phase and amplitude of an electrical signal to enhance spectral efficiency, as for instance, in quadrature modulation. The signal process required for generating and demodulating this kind of signals involves vector processing (phase and amplitude control) and frequency conversion. Unlike the common electronic or digital implementation, in this thesis, different photonic based signal processing techniques are studied to produce digital modulation (photonic vector modulation, PVM) and demodulation (PVdM). These techniques are of particular interest in the case of broadband signals where the data rate required to be managed is in the order of gigabit per second, for applications like wireless backhauling of metro optical networks (known as fibre-to-the-air). The techniques described use optical dispersion in optical fibres, wavelength division multiplexing and photonic up/down conversion. Additionally, an optical heterodyne solution implemented monolithically in a photonic integrated circuit (PIC) is also described.<br>[ES] El procesamiento de señales de radiofrecuencia (RF) utilizando medios fotónicos es una disciplina que apareció casi al mismo tiempo que el láser y la fibra óptica. La fotónica ofrece la capacidad de manipular señales de radiofrecuencia de banda ancha, una baja atenuación, procesados basados en una amplia variedad de fenómenos lineales y no lineales y, recientemente, el potencial para implementar subsistemas fotónicos integrados. Estas características ofrecen un gran potencial para la implementación de múltiples funcionalidades incluyendo transporte óptico, conversión de frecuencia, filtrado óptico de RF, multiplexación y demultiplexación de señales, encaminamiento y conmutación, muestreo óptico, generación de tonos, líneas de retardo, conformación de haz en agrupaciones de antenas o generación fotónica de modulaciones digitales, e incluso una combinación de varias de estas funcionalidades. Esta tesis se centra en la aplicación del procesamiento vectorial en el dominio óptico de señales de radiofrecuencia en dos campos de aplicación: la conformación óptica de haces y la modulación y demodulación vectorial fotónica de señales digitales en cuadratura. El control fotónico vectorial permite manipular la amplitud y fase de las señales de radiofrecuencia en el dominio óptico, que es el procesamiento fundamental que se requiere en diferentes aplicaciones tales como las redes de conformación de haces para agrupaciones de antenas y en la modulación en cuadratura. El trabajo descrito en esta tesis incluye diferentes técnicas para implementar una versión fotónica de las redes de conformación de haces de en agrupaciones de antenas, conocidas como redes ópticas de conformación de haces (OBFN). Se estudian dos familias de redes: arquitecturas de retardo en fibra óptica y arquitecturas integradas. Las primeras permiten el control de señales de banda ancha utilizando fibras ópticas dispersivas con técnicas de multiplexado por división de longitud de onda y funcionalidades avanzadas tales como la estimación del ángulo de llegada de la señal en la antena receptora. En la segunda, se estudian redes de conformación pasivas basadas en Matrices de Butler ópticas integradas, incluyendo una solución ultra-compacta utilizando técnicas ópticas heterodinas en silicio sobre aislante (SOI), y una alternativa homodina en sílice dopado con germanio. En esta tesis, también se han investigado técnicas de procesado vectorial fotónico para la generación de modulaciones digitales en cuadratura. Las modulaciones multinivel codifican la información digital en estados discretos de fase y amplitud de una señal eléctrica para aumentar su eficiencia espectral, como por ejemplo la modulación en cuadratura. El procesado necesario para generar y demodular este tipo de señales implica el procesamiento vectorial (control de amplitud y fase) y la conversión de frecuencia. A diferencia de la implementación electrónica o digital convencional, en esta tesis se estudian diferentes técnicas de procesado fotónico tanto para la generación de modulaciones digitales (modulación vectorial fotónica, PVM) como para su demodulación (PVdM). Esto es de particular interés en el caso de señales de banda ancha, donde la velocidad de datos requerida es del orden de gigabits por segundo, para aplicaciones como backhaul inalámbrico de redes ópticas metropolitanas (conocida como fibra hasta el aire). Las técnicas descritas se basan en explotar la dispersión cromática de la fibra óptica, la multiplexación por división de longitud de onda y la conversión en frecuencia. Además, se presenta una solución heterodina implementada monolíticamente en un circuito integrado fotónico (PIC).<br>[CAT] El processament de senyals de radiofreqüència (RF) utilitzant mitjans fotònics és una disciplina que va aparèixer gairebé al mateix temps que el làser i la fibra òptica. La fotònica ofereix la capacitat de manipular senyals de radiofreqüència de banda ampla, una baixa atenuació, processats basats en una àmplia varietat de fenòmens lineals i no lineals i, recentment, el potencial per implementar subsistemes fotònics integrats. Aquestes característiques ofereixen un gran potencial per a la implementació de múltiples funcionalitats incloent transport òptic, conversió de freqüència, filtrat òptic de RF, multiplexació i demultiplexació de senyals, encaminament i commutació, mostreig òptic, generació de tons, línies de retard, conformació de feix en agrupacions d'antenes i la generació fotònica de modulacions digitals, i fins i tot una combinació de diverses d'aquestes funcionalitats. Aquesta tesi es centra en l'aplicació del processament vectorial en el domini òptic de senyals de radiofreqüència en dos camps d'aplicació: la conformació òptica de feixos i la modulació i demodulació vectorial fotònica de senyals digitals en quadratura. El control fotònic vectorial permet manipular l'amplitud i la fase dels senyals de radiofreqüència en el domini òptic, que és el processament fonamental que es requereix en diferents aplicacions com ara les xarxes de conformació de feixos per agrupacions d'antenes i en modulació multinivell. El treball descrit en aquesta tesi inclou diferents tècniques per implementar una versió fotònica de les xarxes de conformació de feixos en agrupacions d'antenes, conegudes com a xarxes òptiques de conformació de feixos (OBFN), amb els objectius de proporcionar un control precís en aplicacions terrestres de senyals de banda ampla a freqüències molt altes per sobre de 40 GHz en antenes de comunicacions, optimitzant la mida i el pes quan es compara amb els homòlegs elèctrics en aplicacions espacials, i la presentació de noves funcionalitats fotòniques per agrupacions d'antenes. Per tant, s'estudien dues famílies de OBFNs: arquitectures de retard en fibra òptica i arquitectures integrades. Les primeres permeten el control de senyals de banda ampla utilitzant fibres òptiques dispersives amb tècniques de multiplexació per divisió en longitud d'ona i funcionalitats avançades com ara l'estimació de l'angle d'arribada del senyal a l'antena receptora. A la segona, s'estudien xarxes de conformació passives basades en Matrius de Butler òptiques en fotònica integrada, incloent una solució ultra-compacta utilitzant tècniques òptiques heterodinas en silici sobre aïllant (SOI), i una alternativa homodina en sílice dopat amb germani. D'altra banda, també s'ha investigat en aquesta tesi tècniques de processament vectorial fotònic per a la generació de modulacions digitals en quadratura. Les modulacions multinivell codifiquen la informació digital en estats discrets de fase i amplitud d'un senyal elèctric per augmentar la seva eficiència espectral, com ara la modulació en quadratura. El processat necessari per generar i desmodular aquest tipus de senyals implica el processament vectorial (control d'amplitud i fase) i la conversió de freqüència. A diferència de la implementació electrònica o digital convencional, en aquesta tesi s'estudien diferents tècniques de processament fotònic tant per a la generació de modulacions digitals (modulació vectorial fotònica, PVM) com per la seva demodulació (PVdM). Això és de particular interès en el cas de senyals de banda ampla, on la velocitat de dades requerida és de l'ordre de gigabits per segon, per a aplicacions com backhaul sense fils de xarxes òptiques metropolitanes (coneguda com fibra fins l'aire). Les tècniques descrites es basen en explotar la dispersió cromàtica de la fibra òptica, la multiplexació per divisió en longitud d'ona i la conversió en freqüència. A més, es prese<br>Piqueras Ruipérez, MÁ. (2016). Photonic Vector Processing Techniques for Radiofrequency Signals [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/63264<br>TESIS

Phosphorylation of the alpha-subunit of the eukaryotic initiation factor 2 (eIF2alpha) on Serine 51 (Ser51) is an early event associated with downregulation of protein synthesis at the level of translation and constitutes a potent mechanism to overcome various stress conditions. In mammals, four eIF2alpha-kinases PERK, PKR, HRI and GCN2, activated following specific stresses, have been involved in this process. Our laboratory has previously demonstrated that the adaptor protein Nck, composed only of Src homology domains and classically implicated in cell signaling by activated plasma membrane receptor tyrosine kinases, modulates translation through its interaction with the beta-subunit of the eukaryotic initiation factor 2 (eIF2beta). Moreover, we reported that Nck-1 overexpression antagonizes the inhibition of translation in endoplasmic reticulum stress condition and prevents the PERK-mediated phosphorylation of the alpha-subunit of eIF2 on Ser51. In this thesis, I demonstrate that the adaptor protein Nck-1 modulates eIF2alpha-kinase-mediated eIF2alphaSer51 phosphorylation in a specific manner. More particularly, I show that Nck-1 overexpression reduces eIF2alpha phosphorylation in conditions activating PKR or HRI as described previously for PERK. In contrast, I observe that overexpression of Nck-1 in mammalian cells fails to attenuate eIF2alphaSer51 phosphorylation in response to amino acid starvation, a stress condition activating GCN2. I further confirm this observation by showing that Nck-1 fails to alter eIF2alphaSer51 phosphorylation in Saccharomyces cerevisiae, for which the sole eIF2alpha-kinase is GCN2. In addition, I report that Nck-1 reduces PKR activation in response to dsRNA. I also find that Nck-1 reduces dsRNA-induced activation of p38 MAPK, a PKR-downstream substrate, and cell death. Finally, I show that Nck-1 interacts exclusively with the inactivated form of PKR in a Src homology domain independent manner. All together these data uncover the existence of a novel mechanism regulating phosphorylation of eIF2alphaSer51 under various stress conditions and identifies Nck-1 as a modulator of the tumor suppressor and antiviral protein kinase PKR.

Orientador: Elnatan Chagas Ferreira<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia Elétrica e de Computação<br>Made available in DSpace on 2018-08-13T16:12:21Z (GMT). No. of bitstreams: 1 Fernandes_AlecioPontes_M.pdf: 6269465 bytes, checksum: 0d2b04f6049ffb1685b947932894c75d (MD5) Previous issue date: 2009<br>Resumo: Giroscópios são dispositivos usados para medir rotação angular, sendo extensivamente empregados em sistemas de guiamento, navegação e estabilização. Entre os diversos tipos de giroscópios, o IFOG (Giroscópio Interferométrico a Fibra Óptica) baseado no efeito de Sagnac é uma das soluções mais interessante, dada sua alta sensibilidade, resolução, ausência de partes móveis, confiabilidade, tamanho compacto e imunidade a interferências eletromagnéticas. Este trabalho descreve a implementação de um circuito digital de demodulação para IFOGs operando em malha aberta utilizando a técnica de zero-crossing (cruzamento em zero). A técnica implementada no circuito eletrônico descrito neste trabalho emprega um clock quase-síncrono para processamento, o que leva a um esquema de demodulação digital preciso, simples e de baixo custo. O circuito de demodulação proposto foi testado em laboratório, utilizando um sinal de giroscópio emulado. Os resultados experimentais medidos no protótipo mostraram que a resolução final do circuito de detecção é 1000 vezes melhor que a resolução possível de se alcançar em um demodulador zero-crossing digital convencional com clock síncrono<br>Abstract: Gyroscopes are devices used to measure angular orientation, being extensively employed for guidance, navigation and stabilization. Among the many types of gyroscopes, IFOG (Interferometer Fiber Optic Gyroscope), based on Sagnac effect, is the most interesting solution due to its high sensitivity, resolution, reliability, small size and EMI immunity. The present work describes a digital electronic circuit implementation for open-loop IFOGs using zero-crossing demodulation techniques. The technique used in the electronic circuit described in this work employs a quasi-synchronous clock for signal processing which leads to an accurate, simple and low cost digital demodulation scheme. The experimental results of the prototype showed that the final detection resolution is 1000 times better than one achieved by the conventional synchronous clock zero-crossing digital demodulator<br>Mestrado<br>Eletrônica, Microeletrônica e Optoeletrônica<br>Mestre em Engenharia Elétrica