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Journal articles on the topic 'Pathogen enrichment'

Author: Grafiati
Published: 6 September 2023
Last updated: 31 July 2025

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1

MURAKAMI, TAKU. "Filter-Based Pathogen Enrichment Technology for Detection of Multiple Viable Foodborne Pathogens in 1 Day." Journal of Food Protection 75, no. 9 (2012): 1603–10. http://dx.doi.org/10.4315/0362-028x.jfp-12-039.

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Conventional foodborne pathogen assays currently used in the food industry often require long culture enrichments to increase pathogen levels so they can be detected. Even using sensitive real-time PCR assays, culture enrichment at least overnight is necessary especially for detection of pathogens with slow growth rates such as Listeria monocytogenes. To eliminate this cumbersome enrichment step and detect minute amounts of pathogens within 1 day, filter-based pathogen enrichment technology was developed utilizing a unique combination of glass fiber depth filter and porous filter aid materials
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2

Chen, Chin-Yi, Cheryl M. Armstrong, Yiping He, et al. "Multiplexed Detection of Salmonella, Escherichia coli, Campylobacter, and Listeria in Raw Poultry." Foods 14, no. 7 (2025): 1137. https://doi.org/10.3390/foods14071137.

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The detection of foodborne pathogens is a critical aspect of ensuring food safety. Traditional methods rely on time-intensive enrichment steps and pathogen-specific assays, extending testing timelines and limiting throughput. This study evaluates an enrichment-free, multiplexed pathogen detection workflow combining the Pathotrak system for bacterial separation and the Neogen Molecular Detection System (MDS) for detection. The workflow enables simultaneous detection of Salmonella, Escherichia coli O157, Listeria monocytogenes, Listeria spp., and Campylobacter in poultry samples, significantly r
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Lee, Justin S., Ryan S. Mackie, Thomas Harrison, et al. "Targeted Enrichment for Pathogen Detection and Characterization in Three Felid Species." Journal of Clinical Microbiology 55, no. 6 (2017): 1658–70. http://dx.doi.org/10.1128/jcm.01463-16.

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ABSTRACT Traditional diagnostic assays often lack sensitivity and can be difficult to multiplex across many pathogens. Next-generation sequencing (NGS) can overcome some of these problems but has limited application in the detection of low-copy-number pathogens in complex samples. Targeted genome capture (TGC) utilizes oligonucleotide probes to enrich specific nucleic acids in heterogeneous extracts and can therefore increase the proportion of NGS reads for low-abundance targets. While earlier studies have demonstrated the utility of this technology for detection of novel pathogens in human cl
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HUANG, SHISHI, TAY BOON HUI, HYUN-GYUN YUK, and QIANWANG ZHENG. "Development of an Effective Two-Step Enrichment Process to Enhance Bax System Detection of Healthy and Injured Salmonella Enteritidis in Liquid Whole Egg and Egg Yolk." Journal of Food Protection 83, no. 3 (2020): 397–404. http://dx.doi.org/10.4315/0362-028x.jfp-19-280.

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ABSTRACT The BAX system for pathogen detection has been highly accurate in a variety of food products. However, false-negative results have been reported for the detection of pathogens in liquid egg products because of failed pathogen resuscitation and the existence of inhibitory components. In this study, a short-time enrichment step was used to simultaneously resuscitate the target cells to the detection level and to dilute the inhibitory components to reduce detection interference. The MP medium (BAX system) enabled faster multiplication of healthy Salmonella cells than did buffered peptone
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Kim, Hyochin, and Arun K. Bhunia. "SEL, a Selective Enrichment Broth for Simultaneous Growth of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes." Applied and Environmental Microbiology 74, no. 15 (2008): 4853–66. http://dx.doi.org/10.1128/aem.02756-07.

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ABSTRACT Multipathogen detection on a single-assay platform not only reduces the cost for testing but also provides data on the presence of pathogens in a single experiment. To achieve this detection, a multipathogen selective enrichment medium is essential to allow the concurrent growth of pathogens. SEL broth was formulated to allow the simultaneous growth of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes. The results were compared to those obtained with the respective individual selective enrichment broths, Rappaport-Vassiliadis (RV) for S. enterica, modified E. c
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Furtwängler, Anja, Judith Neukamm, Lisa Böhme, et al. "Comparison of target enrichment strategies for ancient pathogen DNA." BioTechniques 69, no. 6 (2020): 455–59. http://dx.doi.org/10.2144/btn-2020-0100.

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In ancient DNA research, the degraded nature of the samples generally results in poor yields of highly fragmented DNA; targeted DNA enrichment is thus required to maximize research outcomes. The three commonly used methods – array-based hybridization capture and in-solution capture using either RNA or DNA baits – have different characteristics that may influence the capture efficiency, specificity and reproducibility. Here we compare their performance in enriching pathogen DNA of Mycobacterium leprae and Treponema pallidum from 11 ancient and 19 modern samples. We find that in-solution approac
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ZHAO, TONG, and MICHAEL P. DOYLE. "Evaluation of Universal Preenrichment Broth for Growth of Heat-Injured Pathogens." Journal of Food Protection 64, no. 11 (2001): 1751–55. http://dx.doi.org/10.4315/0362-028x-64.11.1751.

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Universal preenrichment broth (UPB) was developed to enable enrichment of injured foodborne pathogens of different genera simultaneously in lieu of having to undergo separate simultaneous enrichment cultures for subsequent detection or isolation of each pathogen. Enrichment conditions in UPB for growth of injured pathogens to populations that will enable pathogen detection by rapid immuno-based or polymerase chain reaction (PCR)-based assays have not been defined. Hence, studies were done to determine recovery and growth rates of heat-injured Escherichia coli O157:H7, Salmonella enterica ser.
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Parichehr, Moezi, Kargar Mohammad, Doosti Abbas, and Khoshneviszadeh Mehdi. "Developing a multiplex real-time PCR with a new pre-enrichment to simultaneously detect four foodborne bacteria in milk." Future Microbiology 14, no. 10 (2019): 885–98. http://dx.doi.org/10.2217/fmb-2019-0044.

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Aim: The aim of this study is to formulate a new single nonselective pre-enrichment medium (ELSS) that can support the concurrent growth of four major foodborne pathogens containing E. coli O157: H7, L. monocytogenes, S. aureus and S. enterica serovar Entertidis to develop a multiplex TaqMan Real-time PCR (mRT-PCR). Methods: The mRT-PCR with a new pre-enrichment was carried out for simultaneous detection and quantification of these foodborne bacteria. Results: By using mRT-PCR after 16 h pre-enrichment in ELSS, the detection limit of each pathogen was 1 CFU/25 ml contaminated milk, as well as
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9

Quek, Z. B. Randolph, and Sock Hoon Ng. "Hybrid-Capture Target Enrichment in Human Pathogens: Identification, Evolution, Biosurveillance, and Genomic Epidemiology." Pathogens 13, no. 4 (2024): 275. http://dx.doi.org/10.3390/pathogens13040275.

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High-throughput sequencing (HTS) has revolutionised the field of pathogen genomics, enabling the direct recovery of pathogen genomes from clinical and environmental samples. However, pathogen nucleic acids are often overwhelmed by those of the host, requiring deep metagenomic sequencing to recover sufficient sequences for downstream analyses (e.g., identification and genome characterisation). To circumvent this, hybrid-capture target enrichment (HC) is able to enrich pathogen nucleic acids across multiple scales of divergences and taxa, depending on the panel used. In this review, we outline t
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Hayashi, Masahiro, Tatsuya Natori, Sayoko Kubota-Hayashi, et al. "A New Protocol to Detect Multiple Foodborne Pathogens with PCR Dipstick DNA Chromatography after a Six-Hour Enrichment Culture in a Broad-Range Food Pathogen Enrichment Broth." BioMed Research International 2013 (2013): 1–10. http://dx.doi.org/10.1155/2013/295050.

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A quick foodborne pathogen screening method after six-hour enrichment culture with a broad-range food pathogen enrichment broth is described. Pathogenic factors ofSalmonella enterica,Shigellaspp., enteroinvasiveEscherichia coli, and enterohemorrhagicE. coliare amplified with a cocktail primer and rapid polymerase chain reaction (PCR), which finishes amplification in 30 min. The PCR amplicon was differentiated with a dipstick DNA chromatography assay in 5–10 min. Starting from a four- to six-hour enrichment culture, this assay was finished within 45 min. Detection sensitivity of this protocol w
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TORTORELLO, M. L., K. F. REINEKE, D. S. STEWART, and R. B. RAYBOURNE. "Comparison of Methods for Determining the Presence of Escherichia coli O157:H7 in Apple Juice." Journal of Food Protection 61, no. 11 (1998): 1425–30. http://dx.doi.org/10.4315/0362-028x-61.11.1425.

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Six methods were compared for detection of three strains of Escherichia coli O157:H7 in enrichments of inoculated apple juice. Juice was inoculated at levels varying from 0.1 to 100 CFU/ml and centrifuged after ovemight storage at 4°C, and pellets were incubated at 37°C in nonselective enrichment broth. At hourly intervals between 5 and 10 h and at 24 h, the enrichments were tested for E. coli O157:H7 by direct fluorescent antibody (DFA), antibody-direct epifluorescent filter technique (Ab-DEFT), direct selective plating on sorbitol MacConkey agar (SMA), immunomagnetic separation coupled to ei
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Hahm, Byoung-Kwon, Hyochin Kim, Atul K. Singh, and Arun K. Bhunia. "Pathogen enrichment device (PED) enables one-step growth, enrichment and separation of pathogen from food matrices for detection using bioanalytical platforms." Journal of Microbiological Methods 117 (October 2015): 64–73. http://dx.doi.org/10.1016/j.mimet.2015.07.016.

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13

Mester, Patrick, Martin Wagner, and Peter Rossmanith. "Molecular Enrichment for Qualitative Molecular Pathogen Detection in Food." Food Analytical Methods 11, no. 5 (2017): 1251–56. http://dx.doi.org/10.1007/s12161-017-1103-z.

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Chen, Juan, Junni Tang, Arun K. Bhunia, Cheng Tang, Changting Wang, and Hui Shi. "Development of a multi-pathogen enrichment broth for simultaneous growth of five common foodborne pathogens." Journal of General and Applied Microbiology 61, no. 6 (2015): 224–31. http://dx.doi.org/10.2323/jgam.61.224.

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15

Masud, Numair, Amy Ellison, Edward C. Pope, and Jo Cable. "Cost of a deprived environment – increased intraspecific aggression and susceptibility to pathogen infections." Journal of Experimental Biology 223, no. 20 (2020): jeb229450. http://dx.doi.org/10.1242/jeb.229450.

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ABSTRACTA lack of environmental enrichment can be severely detrimental to animal welfare. For terrestrial species, including humans, barren environments are associated with reduced cognitive function and increased stress responses and pathology. Despite a clear link between increased stress and reduced immune function, uncertainty remains on how enrichment might influence susceptibility to disease. For aquatic vertebrates, we are only now beginning to assess enrichment needs. Enrichment deprivation in fish has been linked to increased stress responses, agonistic behaviour, physiological change
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16

Israeli, Ofir, Efi Makdasi, Inbar Cohen-Gihon, et al. "A rapid high-throughput sequencing-based approach for the identification of unknown bacterial pathogens in whole blood." Future Science OA 6, no. 6 (2020): FSO476. http://dx.doi.org/10.2144/fsoa-2020-0013.

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High-throughput DNA sequencing (HTS) of pathogens in whole blood samples is hampered by the high host/pathogen nucleic acids ratio. We describe a novel and rapid bacterial enrichment procedure whose implementation is exemplified in simulated bacteremic human blood samples. The procedure involves depletion of the host DNA, rapid HTS and bioinformatic analyses. Following this procedure, Y. pestis, F. tularensis and B. anthracis spiked-in samples displayed an improved host/pathogen DNA ratio of 2.5–5.9 orders of magnitude, in samples with bacteria spiked-in at 103–105 CFU/ml. The procedure descri
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Biniaz, Yaser, Ahmad Tahmasebi, Aminallah Tahmasebi, Benedicte Albrectsen, Péter Poczai, and Alireza Afsharifar. "Transcriptome Meta-Analysis Identifies Candidate Hub Genes and Pathways of Pathogen Stress Responses in Arabidopsis thaliana." Biology 11, no. 8 (2022): 1155. http://dx.doi.org/10.3390/biology11081155.

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Following a pathogen attack, plants defend themselves using multiple defense mechanisms to prevent infections. We used a meta-analysis and systems-biology analysis to search for general molecular plant defense responses from transcriptomic data reported from different pathogen attacks in Arabidopsis thaliana. Data from seven studies were subjected to meta-analysis, which revealed a total of 3694 differentially expressed genes (DEGs), where both healthy and infected plants were considered. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis further suggested th
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18

Ebeling, Anne, Alex T. Strauss, Peter B. Adler, et al. "Nutrient enrichment increases invertebrate herbivory and pathogen damage in grasslands." Journal of Ecology 110, no. 2 (2021): 327–39. http://dx.doi.org/10.1111/1365-2745.13801.

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19

Thilliez, Gaetan J. A., Miles R. Armstrong, Tze‐Yin Lim, et al. "Pathogen enrichment sequencing (PenSeq) enables population genomic studies in oomycetes." New Phytologist 221, no. 3 (2018): 1634–48. http://dx.doi.org/10.1111/nph.15441.

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20

Sommermann, Loreen, Doreen Babin, Jan Helge Behr, et al. "Long-Term Fertilization Strategy Impacts Rhizoctonia solani–Microbe Interactions in Soil and Rhizosphere and Defense Responses in Lettuce." Microorganisms 10, no. 9 (2022): 1717. http://dx.doi.org/10.3390/microorganisms10091717.

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The long-term effects of agricultural management such as different fertilization strategies on soil microbiota and soil suppressiveness against plant pathogens are crucial. Therefore, the suppressiveness of soils differing in fertilization history was assessed using two Rhizoctonia solani isolates and their respective host plants (lettuce, sugar beet) in pot experiments. Further, the effects of fertilization history and the pathogen R. solani AG1-IB on the bulk soil, root-associated soil and rhizosphere microbiota of lettuce were analyzed based on amplicon sequencing of the 16S rRNA gene and I
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Conrad, Cheyenne C., Kim Stanford, Tim A. McAllister, James Thomas, and Tim Reuter. "Competition during enrichment of pathogenicEscherichia colimay result in culture bias." FACETS 1, no. 1 (2017): 114–26. http://dx.doi.org/10.1139/facets-2016-0007.

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Deadly outbreaks and illnesses due to Shiga toxin-producing Escherichia coli (STEC) occur worldwide; however, the cultivation methods required for adequate monitoring and traceback investigations are inefficient at best. Detection of STEC relies heavily on enrichment; yet no standard media or protocols exist. Furthermore, whether enrichment may bias detection of multiple STEC serogroups from complex samples is unknown. Thus, 14 STEC strains of serogroups O157 and the top six non-O157s (O26, O45, O103, O111, O121, and O145) were enriched in pairs for 6–78 h in broth and evaluated by quantitativ
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OLIVEIRA, TEREZA C. R. M., SHAI BARBUT, and MANSEL W. GRIFFITHS. "A Robotic DNA Purification Protocol and Real-Time PCR for the Detection of Campylobacter jejuni in Foods." Journal of Food Protection 68, no. 10 (2005): 2131–35. http://dx.doi.org/10.4315/0362-028x-68.10.2131.

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Primers designed to amplify a Campylobacter jejuni cadF gene sequence were used in an SYBR Green I real-time PCR assay as an alternative to conventional bacteriological methods for the rapid detection of C. jejuni in foods. Twenty-five grams of chicken skin (breast and thigh) was contaminated by adding approximately 1, 10, or 50 CFU of C. jejuni ATCC 35560. Twenty-five grams of pork and 25-ml aliquots of milk were also inoculated with 1 and 10 CFU of the pathogen. The samples were incubated in Bolton broth for different periods at 37 and 42°C under microaerophilic conditions. Using a commercia
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Liu, Huifang, Geun Su Noh, Yange Luan, et al. "A Sample Preparation Technique Using Biocompatible Composites for Biomedical Applications." Molecules 24, no. 7 (2019): 1321. http://dx.doi.org/10.3390/molecules24071321.

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Infectious diseases, especially pathogenic infections, are a growing threat to public health worldwide. Since pathogenic bacteria usually exist in complex matrices at very low concentrations, the development of technology for rapid, convenient, and biocompatible sample enrichment is essential for sensitive diagnostics. In this study, a cucurbit[6]uril (CB) supermolecular decorated amine-functionalized diatom (DA) composite was fabricated to support efficient sample enrichment and in situ nucleic acid preparation from enriched pathogens and cells. CB was introduced to enhance the rate and effec
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JARVIS, KAREN G., CHIUN-KANG HSU, JAMES B. PETTENGILL, et al. "Microbiome Population Dynamics of Cold-Smoked Sockeye Salmon during Refrigerated Storage and after Culture Enrichment." Journal of Food Protection 85, no. 2 (2021): 238–53. http://dx.doi.org/10.4315/jfp-21-228.

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ABSTRACT Cold-smoked salmon is a ready-to-eat seafood product of high commercial importance. The processing and storage steps facilitate the introduction, growth, and persistence of foodborne pathogens and spoilage bacteria. The growth of commensal bacteria during storage and once the product is opened also influence the quality and safety of cold-smoked salmon. Here we investigated the microbial community through targeted 16S rRNA gene and shotgun metagenomic sequencing as means to better understand the interactions among bacteria in cold-smoked salmon. Cold-smoked salmon samples were tested
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Berber, Engin, Nurettin Çanakoğlu, İbrahim Sözdutmaz, et al. "Seasonal and Age-Associated Pathogen Distribution in Newborn Calves with Diarrhea Admitted to ICU." Veterinary Sciences 8, no. 7 (2021): 128. http://dx.doi.org/10.3390/vetsci8070128.

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Calf mortality constitutes a substantial loss for agriculture economy-based countries and is also a significant herd problem in developed countries. However, the occurrence and frequency of responsible gastro-intestinal (GI) pathogens in severe newborn diarrhea is still not well known. We aimed to determine the seasonal and age-associated pathogen distribution of severe diarrhea in newborn calves admitted to the intensive care unit (ICU) of Erciyes University animal hospital over a year. Fecal samples were collected during the ICU admissions, and specimens were subjected to a diarrheal pathoge
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van Belkum, Alex, Carina Almeida, Benjamin Bardiaux, et al. "Host-Pathogen Adhesion as the Basis of Innovative Diagnostics for Emerging Pathogens." Diagnostics 11, no. 7 (2021): 1259. http://dx.doi.org/10.3390/diagnostics11071259.

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Infectious diseases are an existential health threat, potentiated by emerging and re-emerging viruses and increasing bacterial antibiotic resistance. Targeted treatment of infectious diseases requires precision diagnostics, especially in cases where broad-range therapeutics such as antibiotics fail. There is thus an increasing need for new approaches to develop sensitive and specific in vitro diagnostic (IVD) tests. Basic science and translational research are needed to identify key microbial molecules as diagnostic targets, to identify relevant host counterparts, and to use this knowledge in
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Wang, Yongjie, Xipan Chen, Xiaohui Xu, et al. "Weighted Gene Co-Expression Network Analysis Based on Stimulation by Lipopolysaccharides and Polyinosinic:polycytidylic Acid Provides a Core Set of Genes for Understanding Hemolymph Immune Response Mechanisms of Amphioctopus fangsiao." Animals 14, no. 1 (2023): 80. http://dx.doi.org/10.3390/ani14010080.

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The primary influencer of aquaculture quality in Amphioctopus fangsiao is pathogen infection. Both lipopolysaccharides (LPS) and polyinosinic:polycytidylic acid (Poly I:C) are recognized by the pattern recognition receptor (PRR) within immune cells, a system that frequently serves to emulate pathogen invasion. Hemolymph, which functions as a transport mechanism for immune cells, offers vital transcriptome information when A. fangsiao is exposed to pathogens, thereby contributing to our comprehension of the species’ immune biological mechanisms. In this study, we conducted analyses of transcrip
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Kadimisetty, Karteek, Kun Yin, Aoife M. Roche, et al. "An integrated self-powered 3D printed sample concentrator for highly sensitive molecular detection of HIV in whole blood at the point of care." Analyst 146, no. 10 (2021): 3234–41. http://dx.doi.org/10.1039/d0an02482a.

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Su, Lv, Lifan Zhang, Duoqian Nie, Eiko E. Kuramae, Biao Shen, and Qirong Shen. "Bacterial Tomato Pathogen Ralstonia solanacearum Invasion Modulates Rhizosphere Compounds and Facilitates the Cascade Effect of Fungal Pathogen Fusarium solani." Microorganisms 8, no. 6 (2020): 806. http://dx.doi.org/10.3390/microorganisms8060806.

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Soil-borne pathogen invasions can significantly change the microbial communities of the host rhizosphere. However, whether bacterial Ralstonia solanacearum pathogen invasion influences the abundance of fungal pathogens remains unclear. In this study, we combined high-throughput sequencing, qPCR, liquid chromatography and soil culture experiments to analyze the rhizosphere fungal composition, co-occurrence of fungal communities, copy numbers of functional genes, contents of phenolic acids and their associations in healthy and bacterial wilt-diseased tomato plants. We found that R. solanacearum
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Zhao, Peng, Jiajin Zhang, Wei Zhang, et al. "Fabrication of a novel hydrogel-based microfluidic chip and its application in pathogen analysis." Analytical Methods 13, no. 43 (2021): 5240–46. http://dx.doi.org/10.1039/d1ay01522b.

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Korsnes, Kjetil, Ove Nicolaisen, Cecilie K. Skår, Audun H. Nerland, and Øivind Bergh. "Bacteria in the gut of juvenile cod Gadus morhua fed live feed enriched with four different commercial diets." ICES Journal of Marine Science 63, no. 2 (2006): 296–301. http://dx.doi.org/10.1016/j.icesjms.2005.10.012.

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AbstractAtlantic cod, Gadus morhua L., larvae were fed rotifers, Brachionus plicatilis and Artemia franciscana enriched on four different commercial media, using the manufacturers' protocols. Pooled samples of 20 cod larvae were homogenized, diluted, and plated out on Petri dishes. The number of colony-forming units per larva was estimated, and the dominant strains subsequently sampled for sequencing of 16S rDNA. Bacteria showing high sequence similarity to a pathogen characteristic of cod and other fish species, Listonella anguillarum, were present in all four groups. Other taxa present among
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Arenas, Ailan F., Gladys E. Salcedo, and Jorge E. Gomez-Marin. "R Script Approach to Infer Toxoplasma Infection Mechanisms From Microarrays and Domain-Domain Protein Interactions." Bioinformatics and Biology Insights 11 (January 1, 2017): 117793221774725. http://dx.doi.org/10.1177/1177932217747256.

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Pathogen-host protein-protein interaction systems examine the interactions between the protein repertoires of 2 distinct organisms. Some of these pathogen proteins interact with the host protein system and may manipulate it for their own advantages. In this work, we designed an R script by concatenating 2 functions called rowDM and rowCVmed to infer pathogen-host interaction using previously reported microarray data, including host gene enrichment analysis and the crossing of interspecific domain-domain interactions. We applied this script to the Toxoplasma-host system to describe pathogen sur
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Caruso, Paola, Jose Luis Palomo, Edson Bertolini, Bel�n �lvarez, Mar�a M. L�pez, and Elena G. Biosca. "Seasonal Variation of Ralstonia solanacearum Biovar 2 Populations in a Spanish River: Recovery of Stressed Cells at Low Temperatures." Applied and Environmental Microbiology 71, no. 1 (2005): 140–48. http://dx.doi.org/10.1128/aem.71.1.140-148.2005.

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ABSTRACT The presence of Ralstonia solanacearum biovar 2 in the watercourses of European countries is increasing, but little is known about its ecology in aquatic habitats. The detection of this pathogen in 2000 in one Spanish river led us to study its population density at different locations on the river over a period of 3 years. During 2000 and 2001, the pathogen was recovered at low densities (10 to 80 CFU/ml) by direct plating on modified SMSA agar from water samples at 14�C or higher, but its isolation was usually unsuccessful at temperatures below 9�C. To monitor the pathogen'
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Lee, Seon-Yeong, Feixiong Chen, and Tae Yoon Lee. "Tryptamine-functionalized magnetic nanoparticles for highly sensitive detection of Salmonella typhimurium." Analyst 146, no. 8 (2021): 2559–66. http://dx.doi.org/10.1039/d0an02458a.

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KOSTIĆ, TANJA, BEATRIX STESSL, MARTIN WAGNER, and ANGELA SESSITSCH. "Microarray Analysis Reveals the Actual Specificity of Enrichment Media Used for Food Safety Assessment." Journal of Food Protection 74, no. 6 (2011): 1030–34. http://dx.doi.org/10.4315/0362-028x.jfp-10-388.

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Microbial diagnostic microarrays are tools for simultaneous detection and identification of microorganisms in food, clinical, and environmental samples. In comparison to classic methods, microarray-based systems have the potential for high throughput, parallelism, and miniaturization. High specificity and high sensitivity of detection have been demonstrated. A microbial diagnostic microarray for the detection of the most relevant bacterial food- and waterborne pathogens and indicator organisms was developed and thoroughly validated. The microarray platform based on sequence-specific end labeli
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LUCHANSKY, JOHN B., ANNA C. S. PORTO, F. MORGAN WALLACE, and JEFFREY E. CALL. "Recovery of Listeria monocytogenes from Vacuum-Sealed Packages of Frankfurters: Comparison of the U.S. Department of Agriculture (USDA) Food Safety and Inspection Service Product Composite Enrichment Method, the USDA Agricultural Research Service (ARS) Product Composite Rinse Method, and the USDA-ARS Package Rinse Method†‡." Journal of Food Protection 65, no. 3 (2002): 567–70. http://dx.doi.org/10.4315/0362-028x-65.3.567.

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This study compared three methods for the recovery of Listeria monocytogenes from commercially prepared and vacuum-packaged frankfurters that were inoculated with a five-strain mixture of this pathogen at averages of 22 and 20,133 CFU per package over three trials. The presence and levels of the pathogen were determined by (i) the U.S. Department of Agriculture (USDA) Food Safety and Inspection Service (FSIS) product composite enrichment method, involving the selective enrichment of a 25-g composite of product and the subsequent plating of this product onto selective agar plates; (ii) the USDA
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Dudenhöffer, Jan‐Hendrik, Stefan Scheu, and Alexandre Jousset. "Systemic enrichment of antifungal traits in the rhizosphere microbiome after pathogen attack." Journal of Ecology 104, no. 6 (2016): 1566–75. http://dx.doi.org/10.1111/1365-2745.12626.

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Lee, Sei Won, Young Ae Kang, Choong Eun Jin, et al. "Gene-based diagnosis of tuberculosis with a new-generation pathogen enrichment technique." European Respiratory Journal 55, no. 3 (2019): 1901885. http://dx.doi.org/10.1183/13993003.01885-2019.

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Parsons, Cameron, Midya Jahanafroozi, and Sophia Kathariou. "Requirement of lmo1930, a Gene in the Menaquinone Biosynthesis Operon, for Esculin Hydrolysis and Lithium Chloride Tolerance in Listeria monocytogenes." Microorganisms 7, no. 11 (2019): 539. http://dx.doi.org/10.3390/microorganisms7110539.

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Listeria monocytogenes is a foodborne pathogen that is widely distributed in nature, having been isolated from a variety of sources such as soil, water, plant matter, and animals. In addition, L. monocytogenes is often detected in the regular sampling of food and food processing environments. The most common method for detecting L. monocytogenes is the use of selective enrichments. Both lithium chloride and esculin, in combination with ferric ammonium citrate, are utilized in several of the most commonly-employed selective enrichment schemes for L. monocytogenes. Here we report that transposon
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Waller, David F., and Steven A. Ogata. "Quantitative Immunocapture PCR Assay for Detection of Campylobacter jejuni in Foods." Applied and Environmental Microbiology 66, no. 9 (2000): 4115–18. http://dx.doi.org/10.1128/aem.66.9.4115-4118.2000.

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ABSTRACT The rapid detection of food-borne bacterial pathogens as part of a quality control program is necessary for the maintenance of a safe food supply. In this report, we present our findings for an immunocapture PCR method for the detection of Campylobacter jejuni in foods. The method permits direct detection of the pathogen without an enrichment step and can be performed in approximately 8 h. Assay results are quantitative, and one cell in a milliliter sample can be detected. Application of the method to spiked milk samples and chicken skin washes did not affect the sensitivity of the as
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Folgueiras-González, Alba, Robin van den Braak, Martin Deijs, Lia van der Hoek, and Ad de Groof. "A Versatile Processing Workflow to Enable Pathogen Detection in Clinical Samples from Organs Using VIDISCA." Diagnostics 11, no. 5 (2021): 791. http://dx.doi.org/10.3390/diagnostics11050791.

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In recent years, refined molecular methods coupled with powerful high throughput sequencing technologies have increased the potential of virus discovery in clinical samples. However, host genetic material remains a complicating factor that interferes with discovery of novel viruses in solid tissue samples as the relative abundance of the virus material is low. Physical enrichment processing methods, although usually complicated, labor-intensive, and costly, have proven to be successful for improving sensitivity of virus detection in complex samples. In order to further increase detectability,
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Vong, Linda, Lee J. Pinnell, Pekka Määttänen, C. William Yeung, Eberhard Lurz, and Philip M. Sherman. "Selective enrichment of commensal gut bacteria protects againstCitrobacter rodentium-induced colitis." American Journal of Physiology-Gastrointestinal and Liver Physiology 309, no. 3 (2015): G181—G192. http://dx.doi.org/10.1152/ajpgi.00053.2015.

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The intestinal microbiota plays a key role in shaping the host immune system. Perturbation of gut microbial composition, termed dysbiosis, is associated with an increased susceptibility to intestinal pathogens and is a hallmark of a number of inflammatory, metabolic, and infectious diseases. The prospect of mining the commensal gut microbiota for bacterial strains that can impact immune function represents an attractive strategy to counteract dysbiosis and resulting disease. In this study, we show that selective enrichment of commensal gut lactobacilli protects against the murine pathogen Citr
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Milton, A. A. P., G. Bhuvana Priya, K. M. Momin, M. Angappan, Samir Das, and S. Ghatak. "An appraisal of various pathogen detection methods in eggs and poultry." Issue 2 (November - December) 1, no. 2 (2020): 93–97. http://dx.doi.org/10.51128/jfas.2020.a017.

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Abstract: To ensure safety in egg and poultry products, timely detection of pathogenic microbes is of paramount importance. This review offers an appraisal of different routinely used and novel emerging pathogen detection methods in egg, poultry and their products. Timely detection of pathogens is decisive to curtail outbreak risks, reduce hospitalisation, and provide product assurance. It will also reduce the cost of holding food products in cold storage and reduces product recalls. Some crucial issues need to be taken care of in choosing or developing a foodborne pathogen detection method. T
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LETELLIER, ANN, S. MESSIER, and S. QUESSY. "Prevalence of Salmonella spp. and Yersinia enterocolitica in Finishing Swine at Canadian Abattoirs." Journal of Food Protection 62, no. 1 (1999): 22–25. http://dx.doi.org/10.4315/0362-028x-62.1.22.

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The prevalence of Salmonella spp. and Yersinia enterocolitica in finishing swine was evaluated using samples of cecal material. Samples were taken at six different slaughterhouses from 1420 healthy, 5-month-old pigs, raised by 223 producers in Québec (1009 samples), Ontario (283), and Manitoba, Canada (128). Two different broth media (Rappaport-Vassiliadis and Tetrathionate brilliant green) were used for the selective enrichment of Salmonella spp. The recovery of Y. enterocolitica was done by a cold enrichment technique, followed by plating on a selective media (cefsulodin-irgasan-novobiocin a
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Marquez, Christine Ardelle, and Kenneth Joseph Bureros. "Pathogen and pesticide contamination in cabbage grown from, Dalaguete, Cebu, Philippines." Palawan Scientist 14, no. 1 (2022): 51–57. http://dx.doi.org/10.69721/tps.j.2022.14.1.06.

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Food safety is one of the long-sought problems in the world. Chemical and biological characteristics must be considered when evaluating the safety of various food products. This study aimed to assess the food safety of cabbages grown from Mantalongon, Dalaguete, Cebu. Cabbage samples acquired from three different farms in Mantalongon were tested for the presence of Salmonella spp. (and other potentially pathogenic bacteria) and pesticide (cypermethrin) residues. To detect the presence of Salmonella spp., pre-enrichment and enrichment methods were done. For the analysis of cypermethrin residue,
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Khanna, Nina, Claudia Stuehler, Barbara Conrad, et al. "Generation of a Multiple Pathogen-Specific T Cell Product for Adoptive Immunotherapy Based on Activation-Dependent Expression of CD154." Blood 116, no. 21 (2010): 2326. http://dx.doi.org/10.1182/blood.v116.21.2326.2326.

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Abstract Abstract 2326 In patients undergoing hematopoietic stem cell transplantation (HSCT) infectious complications are frequent causing substantial morbidity and mortality. Adoptive T cell therapy specific for single pathogens has previously shown to efficiently control viral and fungal infections but approaches targeting multiple pathogens are limited to T cells generated with EBV transformed B cells that are genetically modified expressing multiple viral antigens. Infections are often experienced by different viral and fungal pathogens such as cytomegalovirus (CMV), Epstein-Barr virus (EB
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GOVARIS (Α. ΓΚΟΒΑΡΗΣ), A., D. K. PAPAGEORGIOU (Δ.Κ. ΠΑΠΑΓΕΩΡΓΙΟΥ), and K. PAPATHEODOROU (Κ. ΠΑΠΑΘΕΟΔΩΡΟΥ). "Survival of Escherichia coli 0157:H7 in cultured butter during storage." Journal of the Hellenic Veterinary Medical Society 53, no. 2 (2018): 147. http://dx.doi.org/10.12681/jhvms.15371.

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The survival of E. coli 0157:H7 was examined in 3 types of butter: unsalted, salted with 0.46% and 0.93% salt. The butter samples were inoculated with a low (c.a. 3.14 log CFU/g) or high concentration (c.a. 4.80 log CFU/g) inoculum of the pathogen and were stored at 4°C and 12°C. The contaminated butter samples were stored for 2 months at 4°C and up to visible spoilage (20-26 days) at 12° C. By the end of the storage at 4°C, the tests with the high inoculum of the pathogen revealed that populations of E. coli 0157:H7 were decreased by 2.26 log CFU/g in the unsalted and in 0.46% salted types of
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Leach, Kelly M., Joyce M. Stroot, and Daniel V. Lim. "Same-Day Detection of Escherichia coli O157:H7 from Spinach by Using Electrochemiluminescent and Cytometric Bead Array Biosensors." Applied and Environmental Microbiology 76, no. 24 (2010): 8044–52. http://dx.doi.org/10.1128/aem.01990-10.

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ABSTRACT Contamination of fresh produce with Escherichia coli O157:H7 and other pathogens commonly causes food-borne illness and disease outbreaks. Thus, screening for pathogens is warranted, but improved testing procedures are needed to allow reproducible same-day detection of low initial contamination levels on perishable foods, and methods for detecting numerous pathogens in a single test are desired. Experimental procedures were developed to enable rapid screening of spinach for E. coli O157:H7 by using multiplex-capable immunological assays that are analyzed using biosensors. Detection wa
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ERICKSON, MARILYN C., JYE-YIN LIAO, ALISON S. PAYTON, et al. "Survival of Salmonella enterica and Escherichia coli O157:H7 Sprayed onto the Foliage of Field-Grown Cabbage Plants." Journal of Food Protection 82, no. 3 (2019): 479–85. http://dx.doi.org/10.4315/0362-028x.jfp-18-326.

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ABSTRACT To reduce the number of cabbage pathogen outbreaks, it is essential to understand the fate of enteric pathogens that contaminate plants in the field. To assist in that effort, two independent trials were conducted with a red cultivar (cv. Red Dynasty) and a green cultivar (cv. Bravo F1) of field-grown cabbage (Brassica oleracea var. capitata). In the first trial, plants with small heads were sprayed with an inoculum containing both attenuated Salmonella enterica Typhimurium and Escherichia coli O157:H7 (5.0 log CFU/mL). Initial pathogen levels (ca. 3.9 log CFU per head), determined th
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Rodríguez, Antonio, Brecht Guillemyn, Paul Coucke, and Mario Vaneechoutte. "Nucleic acids enrichment of fungal pathogens to study host-pathogen interactions." Scientific Reports 9, no. 1 (2019). http://dx.doi.org/10.1038/s41598-019-54608-x.

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AbstractFungal infections, ranging from superficial to life-threatening infections, represent a major public health problem that affects 25% of the worldwide population. In this context, the study of host-pathogen interactions within the host is crucial to advance antifungal therapy. However, since fungal cells are usually outnumbered by host cells, the fungal transcriptome frequently remains uncovered. We compared three different methods to selectively lyse human cells from in vitro mixes, composed of Candida cells and peripheral blood mononuclear cells. In order to prevent transcriptional mo
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