Academic literature on the topic 'Phosphate Solubilizing Bacteria (PSB)'

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Journal articles on the topic "Phosphate Solubilizing Bacteria (PSB)"

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Baishya, Ratul, and Rhituporna Saikia. "Phosphate Solubilizing Bacteria in Certain Agricultural Crop Soils of Delhi." INTERNATIONAL JOURNAL OF PLANT AND ENVIRONMENT 4, no. 01 (2018): 70–75. http://dx.doi.org/10.18811/ijpen.v4i01.11613.

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Phosphate solubilizing bacteria (PSB) helps in the solubilization of insoluble phosphates and thus lead to increase in crop yields. A study was conducted to isolate and characterize biochemically PSB from different agricultural crop soils of Delhi such as Garlic, Radish, Chilli, Onion and Cabbage. PSB were isolated in Pikovskaya solid medium and formation of solubilization (halo) zone was measured. 16 PSB were isolated and identified. The selected PSB differed in phosphate solubilizing efficiency, production of organic acids and phosphatases. Citrobacter sp. and Pseudomonas sp. were dominant in all the crop plants. Among all the isolates, Pseudomonas sp. proved to be an efficient phosphate solubilizer.
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Bandita Pati and Sanhita Padhi. "Isolation and characterization of phosphate solubilizing bacteria in saline soil from Costal Region of Odisha." GSC Biological and Pharmaceutical Sciences 16, no. 3 (2021): 109–19. http://dx.doi.org/10.30574/gscbps.2021.16.3.0273.

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The present study was conducted to isolate phosphate solubilizing bacteria (PSB) from rhizospheric saline soils of coastal Odisha, India and evaluated their phosphate solubilizing ability. Total four PSB were isolated based on the halo zone formation (solubilizing index 2.63-3.14) on PVK agar medium and were characterized based on biochemical and molecular characteristics as Bacillus subtilis (B1), B. megaterium (B2), Sphingomonas paucimobilis (P2) and Kocuria kristinae (P6). The inorganic phosphate released by PSB ranged from 18.532 to 38.250µg/ml with decreasing the pH PVK broth up to 3.9. Acid phosphatase activity for PSB were recorded 84.237-98.658µmol/min. Glucose was found to be the best carbon source for B. subtilis, Sphingomonas paucimobilis and Kocuria kristinae whereas mannitol for B. megaterium. Optimum acid phosphatase activity was observed for all the four PSB isolates in presence of ammonium sulphate as nitrogen source in PVK broth at 30oC and pH 7.0.
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Fuskhah, E., and E. D. Purbajanti. "Exploration and selection of salt tolerant phosphate solubilizing bacteria from the Indonesian saline soils." IOP Conference Series: Earth and Environmental Science 1246, no. 1 (2023): 012026. http://dx.doi.org/10.1088/1755-1315/1246/1/012026.

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Abstract The excessive salt concentration is what prevents saline land from being used. Salinity inhibits plant growth, however this can be remedied by investigating phosphate-solubilizing bacteria (PSB). Non-pathogenic bacteria that can dissolve unavailable P into a form that plants may absorb are known as phosphate solubilizing bacteria. The goal of this study is to find phosphate-solubilizing bacteria that can withstand extremely high salinities. In saline land on Indonesia’s North Coast of Central Java, soil sampling was done. Using pikovskaya selective media, phosphate solubilizing bacteria were isolated using the pour plate method. Eight pure PSB isolates, including BPFRBG2, BPFPT2, BPFDMK1, BPFPKL2, BPFSMG2, BPFBTG2, and BPFKDL2, were obtained as a result of the isolation process. The macro- and microscopic examination of PSB isolates. Following closely behind were the PSB isolates with the strongest tolerance to salt (10.000 ppm NaCl): BPFBTG2, BPFKDL2, BPFSMG2, and BPFPKL2.
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Bandita, Pati, and Padhi Sanhita. "Isolation and characterization of phosphate solubilizing bacteria in saline soil from Costal Region of Odisha." GSC Biological and Pharmaceutical Sciences 16, no. 3 (2021): 109–19. https://doi.org/10.5281/zenodo.5542913.

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The present study was conducted to isolate phosphate solubilizing bacteria (PSB) from rhizospheric saline soils of coastal Odisha, India and evaluated their phosphate solubilizing ability. Total four PSB were isolated based on the halo zone formation (solubilizing index 2.63-3.14) on PVK agar medium and were characterized based on biochemical and molecular characteristics as&nbsp;<em>Bacillus subtilis</em>&nbsp;(B1),&nbsp;<em>B. megaterium</em>&nbsp;(B2),&nbsp;<em>Sphingomonas paucimobilis</em>&nbsp;(P2) and&nbsp;<em>Kocuria kristinae</em>&nbsp;(P6). The inorganic phosphate released by PSB ranged from 18.532 to 38.250&micro;g/ml with decreasing the pH PVK broth up to 3.9. Acid phosphatase activity for PSB were recorded 84.237-98.658&micro;mol/min. Glucose was found to be the best carbon source for&nbsp;<em>B. subtilis</em>,&nbsp;<em>Sphingomonas paucimobilis</em>&nbsp;and&nbsp;<em>Kocuria kristinae</em>&nbsp;whereas mannitol for&nbsp;<em>B. megaterium</em>. Optimum acid phosphatase activity was observed for all the four PSB isolates in presence of ammonium sulphate as nitrogen source in PVK broth at 30oC and pH 7.0.
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Hnamte, Remruattluanga, and R. Lalfakzuala. "Phosphate Solubilizing Bacteria in Traditional Jhum Field of Mizoram." Science & Technology Journal 6, no. 1 (2018): 39–44. http://dx.doi.org/10.22232/stj.2018.06.01.05.

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Phosphorus is an essential plant nutrient; however its availability for plant uptake is limited. Phosphate Solubilizing Microbes (PSM) are a group of microbes capable of solubilization of inorganic phosphates thereby making it available for plant use. These microbes (PSM) play a vital role on the phosphorus cycle. Among the PSM, Phosphate Solubilizing Bacteria (PSB) are pre dominant and are crucial for maintaining available phosphorus in soil. However during slash and burning of jhum land, soil microbial community is altered to a great extend. The present study investigates the diversity of PSB in jhum field using 16SrRNA gene amplification of isolated potent bacteria. Seven species of PSB were identified, belonging to Burkholderia, Bacillus and Xanthomonas.
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Amri, Marwa, Mohamed Ridha Rjeibi, Marwa Gatrouni, et al. "Isolation, Identification, and Characterization of Phosphate-Solubilizing Bacteria from Tunisian Soils." Microorganisms 11, no. 3 (2023): 783. http://dx.doi.org/10.3390/microorganisms11030783.

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Soil microorganisms play an important role in maintaining natural ecological balance through active participation in carbon, nitrogen, sulfur, and phosphorous cycles. Phosphate-solubilizing bacteria (PSB) are of high importance in the rhizosphere, enhancing the solubilization of inorganic phosphorus complexes into soluble forms available for plant nutrition. The investigation of this species of bacteria is of major interest in agriculture, as they can be used as biofertilizers for crops. In the present study, 28 isolates of PSB were obtained after the phosphate enrichment of soil samples from five Tunisian regions. Five PSB species were identified by 16S rRNA gene sequencing including Pseudomonas fluorescens, P. putida, and P. taiwanensis, Stenotrophomonas maltophilia, and Pantoea agglomerans. Solid and liquid Pikovskaya’s (PVK) and National Botanical Research Institute’s (NBRIP) media containing insoluble tricalcium phosphate were used for the evaluation of the phosphate solubilization ability of the bacterial isolates by two methods: visual evaluation of the solubilization zone around colonies (halo) and determination of solubilized phosphates in liquid medium by the colorimetric method of the vanado-molybdate yellow. Based on the results of the halo method, the isolate of each species that showed the higher phosphate solubilization index was selected for evaluation of phosphate solubilization by the colorimetric method. In the liquid media, the bacterial isolates showed phosphate solubilization ranging from 535.70 to 618.57 µg mL−1 in the NBRIP medium, and 374.20 to 544.28 µg mL−1 in the PVK medium, with the highest values produced by P. fluorescens. The best phosphate solubilization ability and higher reduction in broth pH, which indicates higher organic acid production, were achieved in NBRIP broth for most of the PSB. Strong correlations were observed between the average capability of PSB to solubilize phosphates and both the pH and total phosphorous content in the soil. The production of the hormone indole acetic acid (IAA), which can promote plant growth, was observed for all five PSB species. Among them, P. fluorescens obtained from the forest soil of northern Tunisia showed the highest production of IAA (50.4 ± 0.9 µg mL−1).
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Asril, Muhammad, Erma Suryanti, and Ika Agus Rini. "In Vitro Evaluation of Phosphate Solubilizing Bacteria as Antagonists for Bacterial Leaf Blight Disease (Xanthomonas oryzae pv. oryzae)." Jurnal Riset Biologi dan Aplikasinya 6, no. 1 (2024): 34–40. http://dx.doi.org/10.26740/jrba.v6n1.p34-40.

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Rice is an important food crop in Indonesia, where more than 50% of the Indonesian population consumes rice as a staple food. Rice has decreased productivity by 50-80% due to bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae (Xoo). The rapid spread and damaging effects of pesticide use on the environment are the main obstacles in the treatment of these bacteria. Alternative control is to use biological agents in the form of phosphate solubilizing bacteria (PSB). Phosphate solubilizing bacteria are part of the growth-promoting bacteria that have other abilities, namely biocontrol. The purpose of this study was to develop the ability of acid soil phosphate solubilizing bacteria to inhibit growth in vitro. The test was carried out using the dual culture technique with the zone of inhibition method. The results showed that ten isolates of PSB had inhibition against Xoo with various diameters of inhibition. The four highest inhibitory isolates were EF.NAP 3 (5.267 ± 0.189 mm), Pseudomonas GSP 6 (4533 ± 0.772 mm), EF.NAP 4 (3,424 ± 0.161 mm) and EF. NAP 9 (3.167±0.136 mm). The ability of PSB to inhibit Xoo can be used as an alternative in controlling bacterial leaf blight in rice plants. This indicates that the PSB isolate acts as a biostimulant in the dissolution of phosphate in the soil and as a bioprotectant.
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ALFIAH, LUFITANUR, DELITA ZUL, and NELVIA NELVIA. "The effect of combination of indigenous phosphate solubilizing bacteria of Riau, Indonesia on the available phosphorus and phosphorus uptake of soybean." Nusantara Bioscience 10, no. 3 (2018): 146–50. http://dx.doi.org/10.13057/nusbiosci/n100303.

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Alfiah L, Zul D, Nelvia N. 2018. The effect of combination of indigenous phosphate solubilizing bacteria of Riau, Indonesia on the available phosphorus and phosphorus uptake of soybean. Nusantara Bioscience 10: 146-150. Despite the abundant amount of phosphorus (P) in the soil, P uptake by the plants is very limited. In acidic soil, phosphorus (P) is bound to aluminum (Al) and iron (Fe), whereas in the alkaline soil, phosphorus (P) is bound to calcium (Ca). The improvement of efficiency and availability of P to plants can be made by utilizing a group of solubilizing phosphate microorganisms. Potency test to investigate the P solubility by Phosphate Solubilizing Bacteria (PSB) has been conducted by isolating the bacteria from peat soil at Biosphere Reserves of Giam Siak Kecil Bukit Batu, Riau, Indonesia. The semi-quantitative test revealed that the PSB were able to dissolve Ca3 (PO4)2, FePo4 and phosphate rock. However, the adaptation ability and potency of PSB from indigenous Riau peat soil inoculated into soybean (Glycine max L. Merr) plants in the mineral soil have not yet been investigated. The present study was carried out from March to June 2015 on the alluvial soil in Babussalam Village, Rambah Sub-district, Rokan Hulu District, Riau. The aim of this study was to determine the effect of PSB inoculation on bacterial population and phosphatase activity. The study also aimed to determine the available P and P uptake and their impact on soybean growth and production. The study employed a factorial experiment laid out in a completely randomized design (CRD) consisted of two factors, i.e., soil treatment and PSB. The first factor comprised of two levels, i.e., T0: non-sterilized soil, T1: sterilized soil. The second factor consisted of 4 levels, i.e., B0: without PSB inoculation, B1: inoculation using 2 isolates of PSB (BB_UB6 and BB_K9), B2: inoculation using 3 isolates of PSB (BB_UB6, BB_K9 and BB_K2), and B3: inoculation using 4 isolates of PSB (BB_UB6, BB_K9, BB_K2, and BB_HS13). The results showed that inoculation of starter 3 had the highest phosphatase activity rate of 12.43 μg p NP g-1 hour-1. The highest available P was produced by starter 2, while the P uptake on non-sterilized soil was higher than that on the sterilized soil at 2.63 mg plant-1. PSB inoculation and soil sterilization did not significantly affect the population of phosphate solubilizing bacteria.
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Susilowati, L. E., and S. Syekhfani. "Characterization of phosphate solubilizing bacteria isolated from Pb contaminated soils and their potential for dissolving tricalcium phosphate." Journal of Degraded and Mining Lands Management 1, no. 2 (2014): 57–62. http://dx.doi.org/10.15243/jdmlm.2014.012.057.

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Phosphorus solubilizing bacteria (PSB) enhances P availability in soils through dissolving inorganic P pools. The characteristics of PSB and the potential of three Pb tolerant phosphate solubilizing rhizobacteria, i.e. Pseudomonassp, Bacillus sp., and Actinomycites sp. were evaluated. PSB were isolated from soil samples contaminated with 300 and 500 mg Pb/kg after incubation for 30 days. Phosphate solubilizing bacteria were screened on for phosphate solubilisation ability in Pikovskaya agar medium (PA). In addition, two of the three indentified PSB strains (Pseudomonas sp. and Bacillus sp.) were characterized for their ability to solubilize tricalcium phosphate in Pikoskaya broth (PB) and also were examined their growth during culture medium incubation. The isolates exhibited different phosphate solubilization index, ranging from 1.87 to 2.98. Pseudomonas sp. had the highest ability to solubilize tricalcium phosphate: 9.82 mg P/ L and 12.23 mg P/ Lin Pikoskaya broth following the addition of 4 mg Pb/L and 2 mg /Pb L, respectively.Â
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Yanti Ningtyas, Dewi Nurma, Betty Natalie Fitriatin, Mieke Rochimi Setiawati, and Tualar Simarmata. "Pengaruh Pemupukan Bakteri Pelarut Fosfat Dan Dosis Batuan Fosfat Terhadap Ketersediaan Fosfor Pada Tanah Budidaya Tanaman Jagung (Zea Mays L.) Di Ultisol Kentrong." Jurnal Ekonomi Pertanian dan Agribisnis 2, no. 1 (2024): 35–40. https://doi.org/10.62379/jepag.v2i1.1941.

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The use of Ultisols for plant cultivation has constraints, namely the nature of the soil is acidic and the availability of phosphate for plants is low. The use of Rocks Phosphate (RP) and Phosphate Solubilizing Bacteria (PSB) can be used as an alternative to increasing the availability of phosphate for environmentally friendly plants. PSB has the potential to increase the solubility and availability of phosphate in plants. The increase in the availability of phosphate for plants can be through the enzyme phosphatase and organic acids produced by PSB. This study aims to examine the effect of the type of PSB consortium and the dose of Rocks Phosphate on P uptake and growth of maize in Ultisols from Kentrong. The research was conducted at the Ciparanje Experimental Garden, Faculty of Agriculture, Padjadjaran University, Jatinangor. The design used was a randomized block design with factorial pattern consisting of phosphate solubilizing bacteria (without the PSB consortium, exogenous PSB consortium, indigenous PSB consortium, and mixed consortium) and Rocks Phosphate dosage (100% SP-36, 100% RP, 80% RP, 60% RP) with three replications. The results showed no interaction between the type of PSB consortium and the dose of Rocks Phosphate. The results of the independent effect showed that the treatment of using SP-36 was the best treatment in available P.
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Dissertations / Theses on the topic "Phosphate Solubilizing Bacteria (PSB)"

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Magallón, Servin Paola. "Development of an inoculant of phosphate rock-solubilizing bacteria to improve maize growth and nutrition." Thesis, Université Laval, 2014. http://www.theses.ulaval.ca/2014/30283/30283.pdf.

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L'utilisation directe de roche phosphatée (RP) est une alternative viable pour remplacer les coûteux fertilisants chimiques dans les pays en voie de développement. L'utilisation de bactéries solubilisatrices de RP (BSRP) est un bon moyen pour augmenter la réactivité de la RP. L'objectif principal de ce travail a été d'obtenir des isolats provenant de la mycorhizosphère du maïs possédant une grande capacité de solubilisation de RP, compatibles avec la mycorhize arbusculaire (MA) et présentant des traits associés aux rhizobactéries favorisant la croissance de plantes (RFCP) pour le développement d'un inoculant bactérien pour le maïs.À partir de 118 isolats obtenus de maïs biologique cultivé au Québec, huit BSRP ont été identifiés comme Asaia lannaensis Vb1, Pseudomonas sp. Vr14, Rahnella aquatilis (Vr7, Vr13 et Sr24) et Pantoea agglomerans (Vr9, Ve16 et Vr25). En milieu liquide, les isolats ont dissous le P des RP selon leur réactivité (Gafsa>Tilemsi>Maroc). La solubilisation des RP s'est effectuée par la production d'acides organiques (OA) et l'abaissement du pH. Les meilleures BSRP de chaque groupe: (A. lannaensis Vb1, Pseudomonas sp. Vr14, R. aquatilis Sr24 et P. agglomerans Vr25) ont été sélectionnées selon leur capacité élevée de solubilisation de la RP et de leur production d'acide indolacétique (AIA) et de sidérophores. L’importance des biofilms formés, ainsi que le degré de motilité variaient selon les isolats et tous étaient compatibles avec le Glomus irregulare (Gi). L’étude de la colonisation des racines montre que R. aquatilis Sr24 et P. agglomerans Vr25 ont été les meilleurs colonisateurs. Lors des expériences en serre, certains mélanges contenant R. aquatilis Sr24, P. agglomerans Vr25 et Gi, ont amélioré la biomasse, l'absorption des nutriments et la colonisation de la plante en association avec un champignon mycorhizien indigène du maïs cultivé dans un sol non stérile et fertilisé avec la RP Marocaine. Nous attribuons ces résultats à leur capacité d'être de bonnes BSRP colonisatrices des racines. Elles sont aussi compatibles avec Gi et sont capables de produire de l'AIA et des sidérophores. Cette thèse démontre donc le potentiel d'utilisation de BSRP comme inoculant afin d'améliorer l'efficacité de l'utilisation directe de RP comme fertilisant phosphaté pour l'agriculture durable du maïs.<br>Phosphorous is the second most important nutrient for plant growth, but its availability is often reduced. Therefore high quantities of expensive soluble P-fertilizers are added to soil. Direct use of phosphate rock (PR) is an alternative to chemical P-fertilizers in developing countries and for sustainable agriculture. In order to increase PR reactivity the use of phosphate rock-solubilizing bacteria (PRSB) is a good alternative. Therefore, the main objective of this work was to obtain mycorrhizosphere-competent PRSB presenting other PGPR-associated traits to be used for the development of an inoculant to improve maize growth and P nutrition. Out of 118 isolates obtained from organically grown maize in Quebec, eight PRSB were identified as Asaia lannaensis Vb1, Pseudomonas sp. Vr14, Rahnella aquatilis (Vr7, Vr13 and Sr24) and Pantoea agglomerans (Vr9, Ve16 and Vr25). All isolates were able to mobilize P from different sparingly soluble P sources in solid media. In liquid medium the isolates were able to solubilize P from PRs according to their reactivity (Gafsa>Tilemsi>Morocco). PRs were solubilized by the production of organic acids (OAs) and by lowering the pH. The best PRSB from each group (A. lannaensis Vb1, Pseudomonas sp. Vr14, R. aquatilis Sr24 and P.3 agglomerans Vr25) were selected based on their high PR solubilization, and capacity for indolacetic acid (IAA) and siderophore production. These four isolates presented different biofilm formation and motility capacities and were compatible with Glomus irregulare (Gi). A root colonization study showed that R. aquatilis Sr24 and P. agglomerans Vr25 were the best root colonizers. Vr25 was very competitive when used with other PRSB. In greenhouse trials, plant inoculation with R. aquatilis Sr24 and P. agglomerans Vr25 in addition to Gi, increased the biomass, nutrient uptake in non-sterile soil amended with Moroccan PR (MPR). We attribute these results not only to their PR-solubilizing capacity but also for their ability to be good PRSB, competitive root colonizers, compatible with Gi and to produce IAA and siderophores. This thesis shows that PRSB with AM fungi can be used as inoculants to improve the efficiency of the direct use of PR as P fertilizer for sustainable maize production.
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Magallón-Servin, Paola, and Paola Magallón-Servin. "Development of an inoculant of phosphate rock-solubilizing bacteria to improve maize growth and nutrition." Doctoral thesis, Université Laval, 2014. http://hdl.handle.net/20.500.11794/24965.

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L'utilisation directe de roche phosphatée (RP) est une alternative viable pour remplacer les coûteux fertilisants chimiques dans les pays en voie de développement. L'utilisation de bactéries solubilisatrices de RP (BSRP) est un bon moyen pour augmenter la réactivité de la RP. L'objectif principal de ce travail a été d'obtenir des isolats provenant de la mycorhizosphère du maïs possédant une grande capacité de solubilisation de RP, compatibles avec la mycorhize arbusculaire (MA) et présentant des traits associés aux rhizobactéries favorisant la croissance de plantes (RFCP) pour le développement d'un inoculant bactérien pour le maïs.À partir de 118 isolats obtenus de maïs biologique cultivé au Québec, huit BSRP ont été identifiés comme Asaia lannaensis Vb1, Pseudomonas sp. Vr14, Rahnella aquatilis (Vr7, Vr13 et Sr24) et Pantoea agglomerans (Vr9, Ve16 et Vr25). En milieu liquide, les isolats ont dissous le P des RP selon leur réactivité (Gafsa&gt; Tilemsi&gt; Maroc). La solubilisation des RP s'est effectuée par la production d'acides organiques (OA) et l'abaissement du pH. Les meilleures BSRP de chaque groupe: (A. lannaensis Vb1, Pseudomonas sp. Vr14, R. aquatilis Sr24 et P. agglomerans Vr25) ont été sélectionnées selon leur capacité élevée de solubilisation de la RP et de leur production d'acide indolacétique (AIA) et de sidérophores. L’importance des biofilms formés, ainsi que le degré de motilité variaient selon les isolats et tous étaient compatibles avec le Glomus irregulare (Gi). L’étude de la colonisation des racines montre que R. aquatilis Sr24 et P. agglomerans Vr25 ont été les meilleurs colonisateurs. Lors des expériences en serre, certains mélanges contenant R. aquatilis Sr24, P. agglomerans Vr25 et Gi, ont amélioré la biomasse, l'absorption des nutriments et la colonisation de la plante en association avec un champignon mycorhizien indigène du maïs cultivé dans un sol non stérile et fertilisé avec la RP Marocaine. Nous attribuons ces résultats à leur capacité d'être de bonnes BSRP colonisatrices des racines. Elles sont aussi compatibles avec Gi et sont capables de produire de l'AIA et des sidérophores. Cette thèse démontre donc le potentiel d'utilisation de BSRP comme inoculant afin d'améliorer l'efficacité de l'utilisation directe de RP comme fertilisant phosphaté pour l'agriculture durable du maïs.<br>L'utilisation directe de roche phosphatée (RP) est une alternative viable pour remplacer les coûteux fertilisants chimiques dans les pays en voie de développement. L'utilisation de bactéries solubilisatrices de RP (BSRP) est un bon moyen pour augmenter la réactivité de la RP. L'objectif principal de ce travail a été d'obtenir des isolats provenant de la mycorhizosphère du maïs possédant une grande capacité de solubilisation de RP, compatibles avec la mycorhize arbusculaire (MA) et présentant des traits associés aux rhizobactéries favorisant la croissance de plantes (RFCP) pour le développement d'un inoculant bactérien pour le maïs.À partir de 118 isolats obtenus de maïs biologique cultivé au Québec, huit BSRP ont été identifiés comme Asaia lannaensis Vb1, Pseudomonas sp. Vr14, Rahnella aquatilis (Vr7, Vr13 et Sr24) et Pantoea agglomerans (Vr9, Ve16 et Vr25). En milieu liquide, les isolats ont dissous le P des RP selon leur réactivité (Gafsa&gt; Tilemsi&gt; Maroc). La solubilisation des RP s'est effectuée par la production d'acides organiques (OA) et l'abaissement du pH. Les meilleures BSRP de chaque groupe: (A. lannaensis Vb1, Pseudomonas sp. Vr14, R. aquatilis Sr24 et P. agglomerans Vr25) ont été sélectionnées selon leur capacité élevée de solubilisation de la RP et de leur production d'acide indolacétique (AIA) et de sidérophores. L’importance des biofilms formés, ainsi que le degré de motilité variaient selon les isolats et tous étaient compatibles avec le Glomus irregulare (Gi). L’étude de la colonisation des racines montre que R. aquatilis Sr24 et P. agglomerans Vr25 ont été les meilleurs colonisateurs. Lors des expériences en serre, certains mélanges contenant R. aquatilis Sr24, P. agglomerans Vr25 et Gi, ont amélioré la biomasse, l'absorption des nutriments et la colonisation de la plante en association avec un champignon mycorhizien indigène du maïs cultivé dans un sol non stérile et fertilisé avec la RP Marocaine. Nous attribuons ces résultats à leur capacité d'être de bonnes BSRP colonisatrices des racines. Elles sont aussi compatibles avec Gi et sont capables de produire de l'AIA et des sidérophores. Cette thèse démontre donc le potentiel d'utilisation de BSRP comme inoculant afin d'améliorer l'efficacité de l'utilisation directe de RP comme fertilisant phosphaté pour l'agriculture durable du maïs.<br>Phosphorous is the second most important nutrient for plant growth, but its availability is often reduced. Therefore high quantities of expensive soluble P-fertilizers are added to soil. Direct use of phosphate rock (PR) is an alternative to chemical P-fertilizers in developing countries and for sustainable agriculture. In order to increase PR reactivity the use of phosphate rock-solubilizing bacteria (PRSB) is a good alternative. Therefore, the main objective of this work was to obtain mycorrhizosphere-competent PRSB presenting other PGPR-associated traits to be used for the development of an inoculant to improve maize growth and P nutrition. Out of 118 isolates obtained from organically grown maize in Quebec, eight PRSB were identified as Asaia lannaensis Vb1, Pseudomonas sp. Vr14, Rahnella aquatilis (Vr7, Vr13 and Sr24) and Pantoea agglomerans (Vr9, Ve16 and Vr25). All isolates were able to mobilize P from different sparingly soluble P sources in solid media. In liquid medium the isolates were able to solubilize P from PRs according to their reactivity (Gafsa&gt; Tilemsi&gt; Morocco). PRs were solubilized by the production of organic acids (OAs) and by lowering the pH. The best PRSB from each group (A. lannaensis Vb1, Pseudomonas sp. Vr14, R. aquatilis Sr24 and P.3 agglomerans Vr25) were selected based on their high PR solubilization, and capacity for indolacetic acid (IAA) and siderophore production. These four isolates presented different biofilm formation and motility capacities and were compatible with Glomus irregulare (Gi). A root colonization study showed that R. aquatilis Sr24 and P. agglomerans Vr25 were the best root colonizers. Vr25 was very competitive when used with other PRSB. In greenhouse trials, plant inoculation with R. aquatilis Sr24 and P. agglomerans Vr25 in addition to Gi, increased the biomass, nutrient uptake in non-sterile soil amended with Moroccan PR (MPR). We attribute these results not only to their PR-solubilizing capacity but also for their ability to be good PRSB, competitive root colonizers, compatible with Gi and to produce IAA and siderophores. This thesis shows that PRSB with AM fungi can be used as inoculants to improve the efficiency of the direct use of PR as P fertilizer for sustainable maize production.<br>Phosphorous is the second most important nutrient for plant growth, but its availability is often reduced. Therefore high quantities of expensive soluble P-fertilizers are added to soil. Direct use of phosphate rock (PR) is an alternative to chemical P-fertilizers in developing countries and for sustainable agriculture. In order to increase PR reactivity the use of phosphate rock-solubilizing bacteria (PRSB) is a good alternative. Therefore, the main objective of this work was to obtain mycorrhizosphere-competent PRSB presenting other PGPR-associated traits to be used for the development of an inoculant to improve maize growth and P nutrition. Out of 118 isolates obtained from organically grown maize in Quebec, eight PRSB were identified as Asaia lannaensis Vb1, Pseudomonas sp. Vr14, Rahnella aquatilis (Vr7, Vr13 and Sr24) and Pantoea agglomerans (Vr9, Ve16 and Vr25). All isolates were able to mobilize P from different sparingly soluble P sources in solid media. In liquid medium the isolates were able to solubilize P from PRs according to their reactivity (Gafsa&gt; Tilemsi&gt; Morocco). PRs were solubilized by the production of organic acids (OAs) and by lowering the pH. The best PRSB from each group (A. lannaensis Vb1, Pseudomonas sp. Vr14, R. aquatilis Sr24 and P.3 agglomerans Vr25) were selected based on their high PR solubilization, and capacity for indolacetic acid (IAA) and siderophore production. These four isolates presented different biofilm formation and motility capacities and were compatible with Glomus irregulare (Gi). A root colonization study showed that R. aquatilis Sr24 and P. agglomerans Vr25 were the best root colonizers. Vr25 was very competitive when used with other PRSB. In greenhouse trials, plant inoculation with R. aquatilis Sr24 and P. agglomerans Vr25 in addition to Gi, increased the biomass, nutrient uptake in non-sterile soil amended with Moroccan PR (MPR). We attribute these results not only to their PR-solubilizing capacity but also for their ability to be good PRSB, competitive root colonizers, compatible with Gi and to produce IAA and siderophores. This thesis shows that PRSB with AM fungi can be used as inoculants to improve the efficiency of the direct use of PR as P fertilizer for sustainable maize production.
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Thurston, Molly Adair. "Identification of phosphate solubilizing bacteria and evaluation of their application with insoluble phosphorus fertilizers to soils from certified organic orchards affected by replant disease." Thesis, University of British Columbia, 2013. http://hdl.handle.net/2429/44949.

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Replant disease (RD) affects the growth and establishment of young fruit trees in old orchard soils. Organic management strategies are needed as an alternative to chemical controls. Improved phosphorus (P) nutrition to stimulate root growth and improve tree establishment is a well-established strategy; however, only insoluble Rock Phosphate (RP) and Bone Meal (BM) inputs are available to Canadian organic growers. The ability of specific plant growth promoting rhizobacteria to solubilize phosphate may improve P availability and its uptake in young apple trees, replanted into inoculated orchards. In this study, 101 bacteria isolated from the roots of legumes from Saskatchewan soils were screened for P solubilization. Thirty-four of these bacteria were positive for P solubilization as measured by halo diameter production on calcium phosphate medium. Twelve isolates showing the largest halo diameters and three known P solubilizing bacteria (PSB) were compared on three media: calcium phosphate, Pikovskaya (PVK) and PVK with bromophenol blue. All twelve isolates previously identified as Pseudomonas, Rhanella, Serratia and Klebsiella spp. solubilized P on all media, although the halo diameters varied among media. The isolates were tested in liquid culture, where a marked decrease in the pH of the solution was observed and six isolates were identified for further testing in growth pouch assays in the presence of insoluble P. The root growth of apple seedlings inoculated with one of the six bacterial isolates, showed significant increases in total root length, surface area and the number of root tips compared to the control after four weeks of incubation. Three isolates were selected for greenhouse bioassays using five RD-affected soils collected from organic orchards. These isolates were inoculated onto apple trees, alone or in combination with RP or BM. Two field trials were simultaneously conducted in organic apple orchards, using iii the same treatments; however there were no significant effects of the isolate treatments in either set of experiments. Although the strongest P solubilizers did not enhance tree growth in the greenhouse and orchard trials, the in vitro work showed the potential of PSB as a tool to mitigate the impact of RD.
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Hsu, Chiu-yen, and 許秋燕. "Characteristics of Phosphate-solubilizing Bacteria and the Effect on the Rhizoplan of Maize by Inoculated with Phosphate- solubilizing Bacteria." Thesis, 1997. http://ndltd.ncl.edu.tw/handle/67597252798847819473.

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碩士<br>國立中興大學<br>土壤學系<br>85<br>abstract Five isolates from soil and nodule of legume were selected by planting maize inoculated with phosphate-solubilizing bacteria in vermiculite to investigate the ability of solubilization inorganic phosphates , the kind of the organic acid in celll excreta and resistance of antiboitics. Next, treatments of different materials- (1)blank, (2)tricalcium phosphate, (3)tricalcium phosphate and humic substance,(4) tricalcium phosphate, humic substance and glucose- with maize were used to investigated the change of population of phosphate-solubilizing bacteria in rhizoplan and the effect on the growth of maize. The result of selecting test showed there were five strains - phosphate-solubilizing bacteria from soil Bacillus pumillus Ca20, Bacillus pumillus Ca48 and Pseudomonas cepacia PSB , Rhizobium fredii SB651 and Rhizobium legueminosarum biovar trifolii V12, which seemed to express better abilities in solubilizingtricalcium phosphate and promoting the dry weight of maize in vermiculite. While incubating with tricalcium phosphate liquid medium for 96 hours, the content of water - soluble phosphorus and population of phosphate- solubilizing bacteria increased as pH decreased for 3~4 units. The supernatant of achieved the lowest pH of 3.8. Result in testing the resistance of antibiotics showed the five isolates can resist teracycline on the concentration of 50 (g mL-1 . So tetracycline were chose to add into tricalcium phosphate plate medium as a method to affirm five isolates besides clear one . Different organic acid and amounts of excreta of five phosphate- solubilizing bacteria were found by analysis of HPLC . Citric acid found in excreta of Pseudomonas cepacia PSB may relates to the mechanism of phosphate solubilizing and decrease of pH in tricalcium phosphate. Planting maize with five different inoculations and four different treatment of materials by sand culture-(1) blank (2) tricalcium phosphate (3) tricalcim phosphate and humic substances, (4) tricalcim phosphate, humic substances and glucose. During five week, the population of phosphate - solubilizing bacteria in the rhizosphere of maize decrease as time increase. The population of phosphate-solubilizing bacteria remained at 105~107 CFU g-1 fresh root . The results indicate there were still phosphate-solubilizing bacteria existing in the rhizosphere of maize. Results in analysis of plant and soil showed treatments of materials and inoculations were not significantly related to phosphorus content of plant, available phosphorus in soil, nitrogen content of plant, dry weight of plants and dry weight of roots. But some inoculations, Rhizobium legueminosarum biovar trifolii V12 and Rhizobium fredii SB651, showed difference on phosphorus content of plant, available phosphorus in soil, nitrogen content of plant. So Planting maize in sand with inoculations of phosphate- solubilizing bacteria for five weeks showed no significant difference on the growth of maize in this experiment. But individual result in phosphorus content of plant, available phosphorus in soil, nitrogen content of plant, dry weight of plants and dry weight of roots showed the potential of phosphate-solubilizing bacteria in promoting growth of maize. Further, dry matter yields in sterile soils less than those in non-sterile soils indicated that sterile soils would depress the growth of maize.
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Xu, Qiu-Yan, and 許秋燕. "Characteristics of Phosphate-solubilizing Bacteria and the Effect on the Rhizoplan of Maize by Inoculated with Phosphate- solubilizing Bacteria." Thesis, 1997. http://ndltd.ncl.edu.tw/handle/94769417869956073870.

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Tsai, Kuo Wei, and 蔡國威. "Characteristic of Tricalcium Phosphate and Ferric Phosphate-solubilizing Activities and Exudates of Bacteria." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/34565822847926010833.

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碩士<br>國立中興大學<br>土壤環境科學系<br>88<br>Calcium phosphate and ferric phosphate are the insoluble phosphate. Phosphate-solubilizing bacteria would solubilize these insoluble phosphates. The phosphate-solubilizing bacteria of this study were incubated in the tricalcium and ferric phosphate liquid medium individually, and analyzed the physiological properties, the content of water-soluble phosphorus, and the exudates of bacteria, and will evaluate the factors of phosphate solubilization of phosphate-solubilizing bacteria. The four phosphate-solubilizing bacteria were screened from 91 isolates from Taiwan are Pseudomonas capecia, Acinetobacter calcoaceticus, Bucillus subtili, and Rhizobium sp.. Four bacteria were incubated in the tricalcium and ferric phosphate liquid medium individually for 120 hours, and sampled per 12 hours. The population changes, pH changes, the changes of the content of water-soluble phosphorus, and the exudates of bacteria were determined. The pH in the tricalcium and ferric phosphate liquid medium would be decreased by four phosphate-solubilizing bacteria. The content of water-soluble phosphorus of A. calcoaceticus in the tricalcium phosphate medium was 511 PO43-, mg L-1 at 108 hour, was the highest of the four bacteria, and the content of water-soluble phosphorus of Rhizobium sp. in the ferric phosphate medium was 73 PO43- , mg L-1, was the highest of the four bacteria. The exudates of the same bacterium in the tricalcium and ferric phosphate liquid medium were very similar, but the contents of the exudates were very different. To calculate the correlation coefficient between the changes of pH, population, the content of exdutaes of the bacteria and the content of water-soluble phosphorus of the tricalcium and ferric phosphate medium during the incubation period. The results indicate there is a negative linear correlation between the changes of pH and the content of water-soluble phosphorus in the tricalcium phosphate medium. There is a linear correlation between the changes of the content of 2-keto-D-gluconic acid, unknown 2 and the content of water-soluble phosphorus in the tricalcium phosphate medium. The result implicated 2-keto-D-gluconic acid and unknown 2 are the factors of solubilizing tricalcium phosphate possibly. There is no linear correlation between the changes of pH and the content of water-soluble phosphorus in the ferric phosphate medium. There is a linear correlation between the changes of the content of 2-keto-D-gluconic acid, D-gluconic acid, unknown 2 and the content of water-soluble phosphorus in the ferric phosphate medium at early incubation period. The result implicated 2-keto-D-gluconic acid, D-gluconic acid, and unknown 2 are the important factors of solubilizing ferric phosphate possibly at early incubation period. The result implicated the mechanism of solubilizing tricalcium phosphate and ferric phosphate are different possibly.
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ZHANG, CHUN-TANG, and 張淳堂. "The characteristics of phosphate-solubilizing bacteria lsolated from Taiwan soils." Thesis, 1992. http://ndltd.ncl.edu.tw/handle/88864313422728868835.

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Tsai, Hsiao-Keng, and 蔡效耕. "Characteristic of Induced Phosphate-solubilizing Activities and Exudates of Bacteria." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/83631179960190763305.

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碩士<br>國立中興大學<br>土壤環境科學系<br>89<br>The non-phosphate-solubilizing bacteria may be induced by metabolites of ether phosphate-solubilizing bacteria. We try to investigate the characteristic of induced phosphate-solubilizing (IPS) activities and exudates of bacteria. The Pseudomonas cepacia Al74 and Escherichia coli JM109 were applied as standard IPS system for measuring the diameter between clear zone and colony. Besides, we analysised of organic acids and water-soluble phosphorus in tricalcium phosphate medium during 180 hr of 5 strain including phosphate- solubilizing bacteria (PSB) strain (Al74, bc29, and bc30), and Rhizobium sp. (A1, and A9) by HPLC and spectrometer after observation the phenomenon of 15 PSB and 24 Rhizobium strain on the tricalcium phosphate solid medium. In addition to activities and exudates study, glucose dehydrogenase (GDH)-related primers were used to study the genotype of 15 PSB strain. The result of IPS on the solid medium is related to bacteria species, and Rhizobium both having having IPS activity and phosphate-solubilizing ability. In standard IPS system, the ratio, which was used in quantilification of the effects of IPS, of phosphate-solubilizing ring to circle was related to IPS distance. In liquid system, co-incubation with E. coli JM109 were reduced the amount of water-soluble phosphorus at the initial stage, and increased later. Except polysaccharid-richness Rhizobium sp. A1, the secretions of Al74, bc29, bc30, and Rhizobium sp. A1 all have secreted 2-keto-D-gluconic acid, which was cope with phosphate-solubilizing hypothesis. The pattern of most PSB PCR products of dehydrogenase-related primers was variety. The primer PqC and GDH used in prediction IPS have correlation value over 0.73, and correlation between PqA and phosphate-solubilizing clear zone is 0.74. Therefore the pyrroloquinoline quinone, cofactor of GDH is one factor in the IPS system.
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Liu, Chun-Yung, and 劉中庸. "The Benefit of using pHospHate solubilizing bacteria enriched compost instead of chemical pHospHate fertilizer—leaf vegetable." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/24052694155920359496.

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碩士<br>國立臺灣大學<br>農業經濟學研究所<br>103<br>The objective of this study was to investigate the effect of different types of soil, addition of phosphorus solubilizing bacteria, and different types of organic fertilizers on the growth of water spinach (Ipomoea aquatica), in order to evaluate the potential of substituting of chemical fertilizers with biological fertilizers, which are composts enhanced with phosphate-solubilizing bacteria (PSB). The results of this study can then be used for the promotion of organic fertilizers in Taiwan. This is a factorial experiment with five soils, five composts, inoculation with or without PSB, and with chemical fertilizer and no fertilizer treatments as controls, using short-term leafy water spinach as the experimental object. The effect of treatments on soil properties and plant nutrient uptakes were analyzed before and after the pot experiments with three replications. Further, the benefits of substituting chemical fertilizers with enhanced phosphate-solubilizing bacterial compost were also discussed based on the results of the experiments. With regards to the benefits obtained by farmers, the substitution of chemical fertilizers with phosphate-solubilizing bacterial compost is technically feasible, and it provides economic benefits to the farmers by increasing the farmer’s income by NT$ 190,000–290,000 per hectare per annum. From the perspective of the government or the domestic industry, substitution of chemical fertilizers with phosphate-solubilizing bacterial compost can decrease government expenditure on chemical fertilizer subsidy, and Taiwan can develop a biological fertilizer industry using phosphate-solubilizing bacteria to promote domestic economic development. In addition, substitution of chemical fertilizers with phosphate-solubilizing bacterial compost is beneficial to public health, and is safer and non-toxic to the environment. This is more conducive to the sustainable development of agriculture and environmental safety in Taiwan, and should therefore be promoted and implemented.
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Huang, Kuo-Hsin, and 黃國鑫. "The Study of Ferulic Acid and p-Hydroxybenzoic Acid Degradation by Phosphate-Solubilizing Bacteria." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/87830223103232380603.

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碩士<br>國立中興大學<br>土壤環境科學系<br>89<br>Recently, the biotechnology is applied to screen microorganism of phenolic compounds of degradation for decreasing allelopathy and increasing crop production. In this study, we approach phosphate-solubilizing bacteria which able to degrade phenolic compounds. Four typical phosphate-solubilizing bacteria strains (A5、C3、Rhodococcus erythroplis and Gordonia sp.) isolated from rhizosphere, were selected from liquid tricalcium phosphate medium with 5000 µg mL-1 ferulic acid and p-hydroxybenzoic acid , respectively. Then, four strains were inoculated to four treatments of two phenolic acid for 240 hours. In this period, we measured C.F.U., pH, water-soluble phosphorus, the degradation of phenolic acids and bioassay. The results showed that pH were all decreased by four strains, especially at Rhodococcus erythroplis and Gordonia sp. strain down to 4.8 in tricalcium phosphate medium with 5000 µg mL-1 p-hydroxybenzoic acid. In opposite, the water-soluble phosphate was increased to 220 and 305 µg mL-1, respectively; however, C.F.U. of four strains were not significantly increased during 240 hours. Beside, only phosphate-solubilizing bacteria Rhodococcus erythroplis strain is able to degrade p-hydroxybenzoic acid. The rate of degradation was 98﹪and the amount of p-hydroxybenzoic acid was down from 3800 to 80 µg mL-1 (0 to 240 hours) and the other three strains were not. In mixed two phenolic acid treatment, the degradation were inhibited showed phenolic acid degradation of phosphate-solubilizing bacteria has specificity. The result of bioassay showed that the seedling of lettuce all inhibited in 5000 µg mL-1 phenolic acid. In conclusion, all results showed phosphate- solubilizing bacteria strains have specificity in degradation of different phenolic compounds.
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Books on the topic "Phosphate Solubilizing Bacteria (PSB)"

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KAUR, Sukhminderjit, and Madhu SHARMA. Studies of Plant Growth Promoting Activities of Phosphate Solubilizing Bacteria from Rhizospheric Soil. Independently Published, 2018.

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Book chapters on the topic "Phosphate Solubilizing Bacteria (PSB)"

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Othman, Radziah, and Qurban Ali Panhwar. "Phosphate-Solubilizing Bacteria Improves Nutrient Uptake in Aerobic Rice." In Phosphate Solubilizing Microorganisms. Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-08216-5_9.

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Jha, Anamika, Sanjay Jha, and Debayan Baidya. "Ecological Diversity, Mechanism, and Biotechnology of Phosphate-Solubilizing Bacteria for Enhanced Crop Production." In Phosphate Solubilizing Microorganisms. Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-08216-5_7.

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Katiyar, Prashant, Sumit Kumar Singh, Sonam Verma, and Sandeep Kumar. "Phosphate-Solubilizing Bacteria: A Role Toward Sustainability in the Agricultural Systems." In Soil Bacteria. Springer Nature Singapore, 2024. http://dx.doi.org/10.1007/978-981-97-3473-3_6.

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Zaidi, Almas, Munees Ahemad, Mohammad Oves, Ees Ahmad, and Mohammad Saghir Khan. "Role of Phosphate-Solubilizing Bacteria in Legume Improvement." In Microbes for Legume Improvement. Springer Vienna, 2010. http://dx.doi.org/10.1007/978-3-211-99753-6_11.

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Zaidi, Almas, Mohammad Saghir Khan, Asfa Rizvi, Saima Saif, Bilal Ahmad, and Mohd Shahid. "Role of Phosphate-Solubilizing Bacteria in Legume Improvement." In Microbes for Legume Improvement. Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-59174-2_8.

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Khan, Mohammad Saghir, Ees Ahmad, Almas Zaidi, and Mohammad Oves. "Functional Aspect of Phosphate-Solubilizing Bacteria: Importance in Crop Production." In Bacteria in Agrobiology: Crop Productivity. Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-37241-4_10.

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Eida, Abdul Aziz, Heribert Hirt, and Maged M. Saad. "Challenges Faced in Field Application of Phosphate-Solubilizing Bacteria." In Rhizotrophs: Plant Growth Promotion to Bioremediation. Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-4862-3_6.

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Arif, Muhammad Saleem, Sher Muhammad Shahzad, Tahira Yasmeen, et al. "Improving Plant Phosphorus (P) Acquisition by Phosphate-Solubilizing Bacteria." In Essential Plant Nutrients. Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-58841-4_21.

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Osorio, Nelson Walter. "Effectiveness of Phosphate Solubilizing Microorganism in Increasing Plant Phosphate Uptake and Growth in Tropical Soils." In Bacteria in Agrobiology: Plant Nutrient Management. Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-21061-7_4.

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Li, Yibin, Mingjiang Zhang, and Xingyu liu. "Application of Phosphate-Solubilizing Bacteria Activating Phosphate Materials in Solidification of Soil Heavy Metal." In Springer Proceedings in Energy. Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-0158-2_71.

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Conference papers on the topic "Phosphate Solubilizing Bacteria (PSB)"

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Panjaitan, Fany, Onesimus Lele, and Rizki Taopan. "Alternative Technology of Phosphorus Supplying for Plant: Mycorrhiza Vesicular-Arbuscular (MVA) and Phosphate Solubilizing Bacteria (PSB)." In Proceedings of the 2nd International Conference on Education, Humanities, Health and Agriculture, ICEHHA 2022, 21-22 October 2022, Ruteng, Flores, Indonesia. EAI, 2023. http://dx.doi.org/10.4108/eai.21-10-2022.2332553.

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Wellala, Harini Chamathka, Ravichandran Vinushayini, Lasantha Herath, and Colin N. Peiris. "Increasing Efficiency of Liquid Fertilizer via Incorporating Beneficial Microorganisms." In SLIIT International Conference on Advancements in Sciences and Humanities 2023. Faculty of Humanities and Sciences, SLIIT, 2023. http://dx.doi.org/10.54389/htea3029.

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The growing trend in organic agriculture has boosted the public awareness of organic fertilizer. The present study focused on isolating plant growth promoting microorganisms from the soil samples and incorporating beneficial plant growth promoting microbial (PGPM) strains to a provided liquid organic fertilizer to improve the efficiency of current formula. After isolating plant growth promoting microorganisms, experiments were conducted qualitatively and quantitatively to evaluate the efficacy of those species. Five phosphorous solubilizing bacteria and fungi, one potassium solubilizing bacteria, one potassium solubilizing fungi, six free living nitrogen fixing bacteria from different regions including Hambanthota, Mahiyanganaya, Galaha, Welimada, Rathnapura Sri Lanka were isolated using serial dilution plating on specific growth media and screened for various plant growth-promoting traits. The highest phosphate solubilization (67.8 mg/ml) was exhibited in PH.1 which also exhibited the highest phosphorous solubilization index (PSI) of 2, isolated from the soil sample received from Hambanthota district. Alginate encapsulation as small beads were produced from bacterial inoculum of PH.1 phosphorous solubilizing bacteria with sodium alginate, cellulose, and calcium chloride. A series of different percentages of cellulose (3% - 6%) was used during bead formation to evaluate the effect of cellulose on encapsulation efficiency of beads. Alginate beads were applied to the liquid fertilizer, incubated, and plated periodically to evaluate the efficiency of this formulation. The number of released cells of PH.1 reached 7.36 X 10 6 CFU/ml after 48 hours of incubation in the 0.25 X diluted liquid fertilizer which resulted from the bead formulation of 4% (w/v) Alginate + 3% (w/v) cellulose. The cellulose supported the entrapment of bacterial cells (plant growth-promoting bacterium) PH.1 as biofertilizer in the matrix, which reduced cell loss. The highest entrapment efficiency of 5.441% was obtained at 3% (w/v) cellulose, Overall, the appropriate content of cellulose mixed with alginate is conducive to changes in the morphology of microcapsules and increases the amount of biological encapsulation. This indicates that the beads-based biofertilizer can partially replace chemical fertilizers.
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Sugiharto, Arwan, Suliasih, Sri Widawati, and Idris. "Phosphate solubilizing bacteria associated with the rhizosphere of sandalwood (Santalum album L.) and their phosphate solubilizing ability." In THE 4TH INTERNATIONAL CONFERENCE ON APPLIED SCIENCES, MATHEMATICS, AND INFORMATICS: ICASMI2022. AIP Publishing, 2024. http://dx.doi.org/10.1063/5.0208195.

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Baranskaya, M. I., L. A. Chaikovskaya, and N. N. Klimenko. "Primary evaluation of a new strain of phosphate-mobilizing bacteria." In CURRENT STATE, PROBLEMS AND PROSPECTS OF THE DEVELOPMENT OF AGRARIAN SCIENCE. Federal State Budget Scientific Institution “Research Institute of Agriculture of Crimea”, 2020. http://dx.doi.org/10.33952/2542-0720-2020-5-9-10-104.

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An initial assessment of new strains of phosphate-mobilizing bacteria isolated from the rhizosphere of cereals was carried out in the laboratory. According to the results of the quantitative evaluation of the phosphate solubilizing activity of bacteria, we found that 12 strains have the greatest ability to transform Ca3(PO4)2 into a soluble form. The highest Кr value was noted for strain 0613: the coefficient of phosphate solubilizing activity – 10. The maximum content of soluble (212 mg/l P2O5) phosphorus was in the culture liquid of strain 1702.
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Yulianti, Evy, and Anna Rakhmawati. "Screening and characterization of phosphate solubilizing bacteria from isolate of thermophilic bacteria." In THE 4TH INTERNATIONAL CONFERENCE ON RESEARCH, IMPLEMENTATION, AND EDUCATION OF MATHEMATICS AND SCIENCE (4TH ICRIEMS): Research and Education for Developing Scientific Attitude in Sciences And Mathematics. Author(s), 2017. http://dx.doi.org/10.1063/1.4995207.

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Arifin, M., N. Nurlaeny, R. Devnita, B. N. Fitriatin, A. Sandrawati, and Y. Supriatna. "The variable charge of andisols as affected by nanoparticles of rock phosphate and phosphate solubilizing bacteria." In THE 1ST INTERNATIONAL CONFERENCE AND EXHIBITION ON POWDER TECHNOLOGY INDONESIA (ICePTi) 2017. Author(s), 2018. http://dx.doi.org/10.1063/1.5021226.

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Fitriatin, Betty, Dahlia Manurung, Emma Sofyan, and Mieke Setiawati. "Synergistic Test and Bioassay of Phosphate Solubilizing Bacteria for Improving Growth of Rice Seedling." In Proceedings of the 1st International Conference on Education, Humanities, Health and Agriculture, ICEHHA 2021, 3-4 June 2021, Ruteng, Flores, Indonesia. EAI, 2021. http://dx.doi.org/10.4108/eai.3-6-2021.2310735.

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Zhang, Wanying, Shuying Wu, Hanshi Guo, et al. "Effects of Phosphate-solubilizing Bacteria on Micro-Tom and Soil of Micro-Tom Rhizosphere." In The International Conference on Biomedical Engineering and Bioinformatics. SCITEPRESS - Science and Technology Publications, 2022. http://dx.doi.org/10.5220/0011198500003443.

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Yang, K. Y., C. Huang, P. Gao, Y. P. Liang, L. J. Han, and X. Zhao. "Identification and Characteristics of Phosphate-Solubilizing Endophytic Bacteria from Root Nodules of Lespedeza Daurica." In XXV International Grassland Congress. International Grassland Congress 2023, 2023. http://dx.doi.org/10.52202/071171-0080.

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Asril, Muhammad, Yuni Lisafitri, Ainin Niswati, et al. "Assessment of Phosphate Solubilization and Indole Acetic Acid Production of Phosphate Solubilizing Bacteria Isolated from Acid Soils, Lampung, Indonesia." In 3rd KOBI Congress, International and National Conferences (KOBICINC 2020). Atlantis Press, 2021. http://dx.doi.org/10.2991/absr.k.210621.080.

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Reports on the topic "Phosphate Solubilizing Bacteria (PSB)"

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Mendoza, Jonathan Alberto, Carolina Mazo, Lina Margarita Conn, Álvaro Rincón Castillo, Daniel Rojas Tapias, and Ruth Bonilla Buitrago. Evaluation of phosphate-solubilizing bacteria associated to pastures of Bracharia from acid soils. Corporación Colombiana de Investigación Agropecuaria - AGROSAVIA, 2015. http://dx.doi.org/10.21930/agrosavia.informe.2015.5.

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Abstract:
Rhizobia have been widely known by their capacity to form a symbiotic relationship with legumes and fix atmospheric nitrogen. Recently, however, rhizobia have shown to associate with plants in different botanical families. In this study, we aimed at elucidating the diversity of rhizobia associated to grasses, and determine their capabilities to solubilize phosphate in both lab and greenhouse experiments. Isolation of rhizobia was performed using rhizosphere from Brachiaria brizantha and B. decumbens and a promiscuous legume trap plant (i.e. Vigna unguiculata). Thirty days after inoculation of the trap plant, rhizobia were isolated from nodules using the conventional protocol, classified in basis on their phenotypic features, and molecularly grouped using Amplified Ribosomal DNA Restriction Analysis (ARDRA). Finally, phosphate solubilization assays and greenhouse experiments were carried out on representatives of each ARDRA cluster. The results showed that the diversity of rhizobia varied between both plant species, which suggests that plant exudates significantly determine the composition of the plant microbiome. Surprisingly, most of the isolated associated to B. brizantha rhizosphere exhibited typical attributes of slow-growing rhizobia, whereas rhizobia from B. decumbens displayed a mixed diversity including slow-, intermediate-, and fast-growing rhizobia. Sequencing of 16S rRNA of ARDRA representatives showed that most of the rhizobia isolated from B. brizantha belonged to the Mesorhizobium and Bradyrhizobium genera, while those isolated from B. decumbens were phylogenetically clustered into Rhizobium and Bradyrhizobium. The capability of the isolates to solubilize phosphate was studied using iron and calcium phosphate. We observed that overall Bradyrhizobium exhibited the highest ability to solubilize iron phosphate; by contrast, calcium phosphate was similarly solubilized within representatives of the three genera. In greenhouse experiments, we found that plants inoculated with isolated BT53, BD17 and BD21 exhibited a significantly higher content of phosphorus (p≤0.05). Additionally, dry weight was significantly higher in the treatment inoculated with BT16 isolate (p≤0.05). We conclude that 1) rhizobia is found associated with grasses, 2) plant genotype determines rhizobia diversity 3) rhizobia are able to solubilize phosphorus, and 4) they might be used to promote plant in different plant families. We further believe that further studies will reveal the true role of those old-known legume symbionts in development and growth of other important crops.
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