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Journal articles on the topic 'RAPD characterization'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Mir, Mudasir A., Amrina Shafi, and Insha Zahoor. "RAPD based Molecular Characterization of Lavatera cachemiriana Cambess." International Journal of Trend in Scientific Research and Development Volume-2, Issue-1 (2017): 377–83. http://dx.doi.org/10.31142/ijtsrd5962.

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Bieliková, L., Z. Landa, L. S. Osborne, and V. Čurn. "Characterization and identification of entomopathogenic and mycoparasitic fungi using RAPD-PCR technique." Plant Protection Science 38, No. 1 (2012): 1–12. http://dx.doi.org/10.17221/4813-pps.

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Entomopathogenic and mycoparasitic fungi were characterised by RAPD technique, with special attention to evaluate the genetic stability of strains that are used as active ingredients in commercial biopesticides. Strain-specific fingerprints were constructed for Paecilomyces fumosoroseus &ndash; strain PFR 97 Apopka, Gliocladium virens &ndash; strain GL 21 and Verticillium lecanii &ndash; strain MYCOTAL. Genetic stability and homogeneity was confirmed among re-isolates that were obtained from commercial batches of bio-insecticide PFR 97TM 20%WDG and bio-fungicide SoilGardTM12G that

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3

Maan Hasan Al_yassine. "Molecular characterization of nine grapevine varieties cultivated in Salahaldin, Iraq by using RAPD-PCR marker." Tikrit Journal of Pure Science 22, no. 2 (2023): 13–18. http://dx.doi.org/10.25130/tjps.v22i2.623.

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Grapevine (Vitis vinifera L.) is one of important economic fruit crops found in Salahaldin province \ Iraq. To examine the Molecular characterization and genetic relationships among nine grape varieties by using random amplified polymorphic DNA (RAPD) marker. Fifteen RAPD primers produced polymorphic band (71, 56%), while two RAPD primers yielded monomorphic bands only. The size of the fragment ranged between 250-2700 bp, with an average of 5.47 band/primer. A total of 20 unique and absent bands used to identify seven cultivars. This study has found that genetic distance values ranged from 0.0

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Ovesná, J., L. Kučera, R. Bocková, and V. Holubec. "Characterization of Powdery Mildew Resistance Donors within Triticum boeoticum Accessions using RAPDs." Czech Journal of Genetics and Plant Breeding 38, No. 3-4 (2012): 117–24. http://dx.doi.org/10.17221/6245-cjgpb.

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Wide hybridization has become a technique suitable for introgression of alien genes into modern wheat cultivars. Ten Triticum boeoticum Boiss. accessions resistant to powdery mildew were studied in more detail. Variability between the accessions was studied using resistance tests, RAPD and storage protein analysis. Potential gene recipients (T. aestivum and T. durum cultivars) were also included. Unlike protein analysis we were able to clearly distinguish among the individual accessions of T.&nbsp;boeotium using RAPD. Computed dendrograms reflect phylogenetic relationships among the charac

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Mudasir, A. Mir, Shafi Amrina, and Zahoor Insha. "RAPD based Molecular Characterization of Lavatera cachemiriana Cambess." International Journal of Trend in Scientific Research and Development 2, no. 1 (2017): 377–83. https://doi.org/10.31142/ijtsrd5962.

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The exploration of genetic diversity is important to properly maintain and exploit germplasm resources and to design better strategically defined approaches in the utilization of collected germplsam so as to safeguard it from extinction. Therefore, current study was undertaken to evaluate genetic diversity of Lavatera cachemiriana collected from different geographical locations. RAPD based molecular markers were used to evaluate genetic diversity. Total DNA was isolated using CTAB method, molecular marker based polymorphism was carried out using ISSR and RAPD molecular markers, while as geneti

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Mor, V. S., D. P. Deswal, A. Mann, B. S. Dahiya, and B. S. Beniwal. "Characterization of marigold (Tagetes spp.) genotypes using SDS-PAGE and RAPD markers." Seed Science and Technology 36, no. 3 (2008): 757–66. http://dx.doi.org/10.15258/sst.2008.36.3.25.

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Abou-Zeid, N. M., H. Halila, and M. S. Khalil. "Characterization by RAPD-PCR of races of Fusarium oxysporum f.sp. ciceri infecting chickpea." Plant Protection Science 38, SI 2 - 6th Conf EFPP 2002 (2017): 297–301. http://dx.doi.org/10.17221/10475-pps.

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Isolates of Fusarium oxysporum f.sp. ciceri from chickpea (Cicer arietnum L.) can be characterized as races and wilt and yellowing pathotypes by pathogenicity tests on varieties of differentials chickpea. This study shows that, DNA bands generated by RAPD-PCR can be used to assign Fusarium oxysporum f.sp. ciceri isolates to pathotype and pathogenic race.

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de Wet, Juanita, Michael J. Wingfield, Teresa A. Coutinho, and Brenda D. Wingfield. "Characterization of Sphaeropsis sapinea Isolates from South Africa, Mexico, and Indonesia." Plant Disease 84, no. 2 (2000): 151–56. http://dx.doi.org/10.1094/pdis.2000.84.2.151.

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Post-hail-associated dieback of Pinus patula and P. radiata trees, induced by Sphaeropsis sapinea (=Diplodia pinea), is a common and important disease in commercial pine plantations. Two morphotypes, A and B, have been described for this fungus based on differences in cultural characteristics, conidial morphology, and virulence among isolates from the north central United States. The existence of the two described morphotypes was later verified through the use of random amplified polymorphic DNA (RAPD) analyses, and the morphotypes were designated as the A and B RAPD marker groups. The objecti

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SILVA, D. R. O., D. AGOSTINETTO, and L. VARGAS. "MOLECULAR CHARACTERIZATION OF HAIRY FLEABANE USING RAPD." Planta Daninha 34, no. 3 (2016): 433–42. http://dx.doi.org/10.1590/s0100-83582016340300004.

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ABSTRACT With the increase of glyphosate resistance evolution in hairy fleabane, the knowledge of genetic diversity is essential to assist in the weed management. The objective was to characterize the genetic diversity of glyphosate resistant Conyza species in Rio Grande do Sul. Hairy fleabane biotype seeds were collected in fields of glyphosate-resistant soybean, generating seedlings used for extracting DNA from the leaves to perform a genetic diversity analysis, using the RAPD technique. The DNA was used in polymerase chain reaction and DNA fragments were assessed for polymorphism bands in e

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Gayathri, Gunalan, A.J.Preethi, P.Sumathi, and Mahadeva Rao U.S. "Molecular characterization of Gymnema sylvestre using RAPD-PCR." International Journal of BioSciences and Technology (IJBST) ISSN: 0974-3987 2, no. 9 (2009): 149–57. https://doi.org/10.5281/zenodo.1438259.

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Abstract   Herbal medicine is playing a major role in the control of many diseases, especially in developing countries like India. The conservation and utilization of these plants has attracted global attention. Diabetes mellitus is a endocrine syndrome which is characterized by insulin insufficiency or inefficiency. Besides many oral hypoglycemic drugs, there are certain anti-diabetic plants which can be used for the treatment of diabetes mellitus. <em>Gymnema sylvestre </em>is one of those anti-diabetic plants. To improve the cultivar of this <em>Gymnema sylvestre, </em>identification o

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Akshitha HJ, Prasath D, Umesha K, Mohammed Faisal P, and Venkataravanappa V. "Molecular characterization of ginger genotypes using RAPD and SSR markers." Journal of Horticultural Sciences 17, no. 1 (2022): 95–102. http://dx.doi.org/10.24154/jhs.v17i1.1052.

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Genetic diversity among ginger genotypes collected from different parts of the country was studied using molecular markers (30 RAPD and 55 SSR). Compared to RAPD primers SSR primers were efficient in distinguishing the genotypes. A total of 86 and 23 polymorphic bands were observed with RAPD and SSR primers, respectively. Percentage polymorphism observed between RAPD and SSR primers was 97.40 % and 56.54 %. Grouping of genotypes by using combined data of RAPD and SSR primers indicated that irrespective of their place of collection or geographical origin, 30 genotypes were clustered into differ

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Dhlamini, Z., J. Mbanga, E. Chauke, and S. Dube. "Characterization of Fasciola gigantica isolates from cattle from South-western Zimbabwe using RAPD-PCR." IOSR Journal of Agriculture and Veterinary Science 7, no. 2 (2014): 19–25. https://doi.org/10.5281/zenodo.2590953.

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The study sought to characterize Fasciola gigantica isolates from cattle in different localities using RAPD-PCR. Adult flukes morphologically identified as F. gigantica were collected from slaughtered infected animals during meat hygiene inspections. DNA was extracted from single flukes and subjected to RAPD-PCR analysis. In the RAPD-PCR analysis, genomic DNA isolated from the conical anterior end of the worms was amplified by the polymerase chain reaction using 10 random oligonucleotide primers. Depending upon the Fasciola gigantica isolate-primer combination, 1-13 DNA fragments in the range

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Chauke, E., J. Mbanga, Z. Dhlamini, and S. Dube. "Characterization of Fasciola gigantica isolates from cattle from South-Western Zimbabwe using RAPD-PCR." IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) 7, no. 2 (2014): 19–25. https://doi.org/10.5281/zenodo.2572489.

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The study sought to characterize Fasciola gigantica isolates from cattle in different localities using RAPD-PCR. Adult flukes morphologically identified as F. gigantica were collected from slaughtered infected animals during meat hygiene inspections. DNA was extracted from single flukes and subjected to RAPD-PCR analysis. In the RAPD-PCR analysis, genomic DNA isolated from the conical anterior end of the worms was amplified by the polymerase chain reaction using 10 random oligonucleotide primers. Depending upon the Fasciola gigantica isolate-primer combination, 1-13 DNA fragments in the range

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14

Sonnante, G., P. Perrino, A. De Polis, and V. Lattanzio. "CHARACTERIZATION OF ARTICHOKE GENOTYPES USING RAPD MARKERS." Acta Horticulturae, no. 681 (June 2005): 301–6. http://dx.doi.org/10.17660/actahortic.2005.681.39.

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15

Bando, Silvia Yumi, Gloria R. F. Valle, Marina B. Martinez, Luiz R. Trabulsi, and Carlos A. Moreira-Filho. "Characterization of enteroinvasiveEscherichia coliandShigellastrains by RAPD analysis." FEMS Microbiology Letters 165, no. 1 (1998): 159–65. http://dx.doi.org/10.1111/j.1574-6968.1998.tb13141.x.

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Theodorakis, Christopher W., and John W. Bickham. "Molecular Characterization of Contaminant-Indicative RAPD Markers." Ecotoxicology 13, no. 4 (2004): 303–9. http://dx.doi.org/10.1023/b:ectx.0000033088.68427.59.

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Huff, David R. "RAPD Characterization of Heterogenous Perennial Ryegrass Cultivars." Crop Science 37, no. 2 (1997): 557–64. http://dx.doi.org/10.2135/cropsci1997.0011183x003700020041x.

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18

Miaja, M. L., R. Vallania, C. Me, A. Akkak, O. Nassi, and G. Lepori. "VARIETAL CHARACTERIZATION IN HAZELNUT BY RAPD MARKERS." Acta Horticulturae, no. 556 (July 2001): 247–50. http://dx.doi.org/10.17660/actahortic.2001.556.35.

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19

Abou-Zeid, N. M., I. H. Dorriah, and A. A. Marwa. "Application of molecular methods for characterization of Botrytis fabae and Botrytis cinerea of faba bean." Plant Protection Science 38, SI 2 - 6th Conf EFPP 2002 (2017): 314–18. http://dx.doi.org/10.17221/10479-pps.

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Application of the RAPD methods allowed to clearly characterization of isolates of Botrytis fabae and isolates of B. cinerea. Results from RAPD-PCR analysis indicate different groups. Clusters were related with groups based on conidial morphology, morphological characters of the isolates of Botrytis spp.

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20

Sanz-Cortés, F., M. L. Badenes, S. Paz, A. Íñiguez, and G. Llácer. "Molecular Characterization of Olive Cultivars Using RAPD Markers." Journal of the American Society for Horticultural Science 126, no. 1 (2001): 7–12. http://dx.doi.org/10.21273/jashs.126.1.07.

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Forty olive (Olea europaea L.) cultivars from Valencia, Spain, were screened using random amplified-polymorphic DNA (RAPD) markers. Eighteen selected decamer primers produced 34 reproducible amplification fragments that were then used as polymorphic markers. The resulting combinations of these RAPD markers were used to discriminate 40 cultivars. Results were analyzed for similarity among cultivars and the relatedness of polymorphisms obtained between cultivars agreed with previous results using isozymes. Unweighted pair group method cluster analysis of their similarity values revealed two main

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21

Rohit, Sharma, and Kumar Sandeep. "MOLECULAR MARKER ASSISTED STUDIES ON GENETIC DIVERSITY IN BRASSICA SPECIES." International Journal of Research -GRANTHAALAYAH 3, no. 7 (2017): 63–71. https://doi.org/10.5281/zenodo.847072.

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Genetic diversity of seven genotypes of Brassica campestris was evaluated by using RAPD primers. A total of 15 DNA bands were revealed wherein 11 polymorphic bands were observed. Highest polymorphism was showed by the primer OPA 7 (87.50%) and lowest by the primer OPA 9 (57.14%). Relatively higher genetic diversity was found among the collected individuals. A maximum similarity based on Jaccard coefficient was showed highest and lowest value (0.750) and (0.364) respectively. The UPGMA cluster analysis revealed three main group of clusters in which one group was found to be sub grouped and othe

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22

Sandhu, Manpreet, Sanjeev Chauhan, Rajni Chauhan, and Kuldeep Singh. "Morphological and molecular characterization of Dendrocalamus Strictus Roxb. accessions." Indian Journal of Forestry 33, no. 3 (2010): 297–303. http://dx.doi.org/10.54207/bsmps1000-2010-q1h059.

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The present study was undertaken to study genetic relationships of sixteen accessions of Dendrocalamus strictus Roxb. on the basis of morphological characters and RAPD markers. Considerable variability was found among the accessions analyzed, yet, no significant relationship exhibited between genetic variation estimated by morphological and RAPD characters. The analysis of genetic relationship in Dendrocalamus strictus using morphological and RAPD banding data can be useful for framing plant improvement, conservation and management strategies.

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Collares, Eliana Antonia Silveira, Eva Choer, and Arione da Silva Pereira. "Characterization of potato genotypes using molecular markers." Pesquisa Agropecuária Brasileira 39, no. 9 (2004): 871–78. http://dx.doi.org/10.1590/s0100-204x2004000900006.

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The objective of this work was to characterize 27 potato genotypes, using molecular markers. Polyacrylamide gel electrophoresis, RAPD techniques and isozymes of esterase, phosphoglucomutase and soluble proteins were analyzed in tubers, and isocitrate dehydrogenase, aspartate transaminase, phosphoglucomutase and peroxidase, in leaves. Eighteen primers were tested and four were chosen, kits OPX (01, 04 and 09) and OPY (07), to analyze RAPD markers in leaf extracts. Similarity and cluster analysis were conducted using Jaccard coefficient and the unweighted pair-group method using arithmetic avera

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Javaria, Altaf. "Genetic diversity analysis of snail species (Mollusca: Gastropoda) using molecular marker technology." Journal of Biodiversity and Environmental Sciences (JBES) 10, no. 1 (2017): 26–35. https://doi.org/10.5281/zenodo.2592029.

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Members of the Phylum <em><strong>Mollusca</strong></em> can be used as model animals to study the reproductive barriers, for evolution and gamete recognition proteins for which they must be correctly identified and classified. Phenotypic plasticity results in changes in shell morphology, leading to the development of synonyms and homonyms of species, which necessitates <em><strong>Molecular Characterization</strong></em>. Fifteen species of molluscs collected from different agro-ecosystems of Faisalabad were characterized using polymorphic RAPD dacamers. Total of 114 fragments amplified and o

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Odinot, P. T., J. F. G. M. Meis, P. J. J. C. Van Den Hurk, J. A. A. Hoogkamp-Korstanje, and W. J. G. Melchers. "PCR-based characterization ofYersinia enterocolitica: comparison with biotyping and serotyping." Epidemiology and Infection 115, no. 2 (1995): 269–77. http://dx.doi.org/10.1017/s0950268800058398.

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SummaryPCR-based DNA fingerprinting was used to characterize 48 clinical isolates ofYersinia enterocolitica. The samples were examined by random amplified polymorphic DNA (RAPD-PCR) and inter-repeat PCR (IR-PCR). IR-PCR with two enterobacterial repetitive intergenic consensus primers resulted in patterns which were poorly discriminated; 2 of 11 arbitrary primers (RAPD-PCR) provided sufficient discriminatory power. In comparisons with serotyping and biotyping, RAPD-fingerprinting was the most discriminatory technique and may therefore be a valuable epidemiological tool for the study ofY. entero

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Mudalige, Chathuri, ST Girisha, VB Raghavendra, MH Niranjan, K. Ravikumar, and MG Sumantha. "Molecular Characterization of Macrophomina phaseolina, the Incitant of Coleus forskohlii Revealed by RAPD Markers." International Journal of Life Sciences 5, no. 1 (2012): 44–50. http://dx.doi.org/10.3126/ijls.v5i1.5598.

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Coleus forskohlii belong to family lamiaceae is one of the commercial plants grown extensively in the country, the chemical found in the Coleus which has both medicinal application and gives great economy to the industrial organizations. Unfortunately, these plants are being highly succumbed to serious diseases like wilt and root rot caused by a fungus, hence the growers and industrialists are facing severe problem in safeguarding this crop in the field irrespective of the agro climatic conditions. Root rot disease, is one of the major diseases of Coleus forskohlii which, is caused by Macropho

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J., O. Popoola, M. Adebayo B., E. Adegbite A., C. Omonhinmin A., and D. Adewale B. "Fruit Morphometric and RAPD Evaluation of Intraspecific Variability in Some Accessions of African Yam Bean (Sphenostylis stenocarpa Hochst. ex. A. Rich. Harms)." Annual Research & Review in Biology 14, no. 4 (2017): 1–10. https://doi.org/10.9734/ARRB/2017/34264.

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African Yam Bean (AYB) (<em>Sphenostylis stenocarp</em>a, Hochst. ex A. Rich, Harms) is an indigenous underutilized legume mainly grown in Sub-saharan African as a source of protein. Intraspecific variability studies were carried out on 10 accessions of AYB obtained from the International Institute of Tropical Agriculture (IITA) Ibadan, Nigeria. Fourteen (14) fruit morphometric characters and nine (9) arbitrary RAPD primers were employed for evaluation of genetic intra-specific variability among the accessions. A total of 410 bands were generated with 261 (63.66%) polymorphic bands. There is s

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Kamel, Ehab, and Mohamad Rashed. "Molecular Characterization of Penicillium Isolates Using Rapd Technique." Annual Research & Review in Biology 7, no. 3 (2015): 185–99. http://dx.doi.org/10.9734/arrb/2015/13657.

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Passos, Leônidas Paixão, Marco Antonio Machado, Maria Coletta Vidigal, and Ana Lúcia Campos. "Molecular characterization of elephantgrass accessions through RAPD markers." Ciência e Agrotecnologia 29, no. 3 (2005): 568–74. http://dx.doi.org/10.1590/s1413-70542005000300009.

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Elephantgrass pastures are limited by yield variations and reductions in forage quality and availability, thus making the search for genotypes with reduced seasonality a major concern. In order to verify the extent of genetic variability among contrasting cultivars, ten elephantgrass accessions were analyzed through DNA amplification by RAPD technique. A total of 160 DNA bands were generated with the use of 44 random primers and 23% of these bands were monomorphic for all accessions. Gel-obtained binary data (1 for presence and 0 for absence) were used for generating a genetic distance matrix,

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Uzun, A., O. Gulsen, Y. AkaKacar, et al. "Characterization of new apricot cultivars by RAPD markers." Journal of Applied Horticulture 09, no. 02 (2007): 132–35. http://dx.doi.org/10.37855/jah.2007.v09i02.29.

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Deshmukh, Radhika, P. N. Davhale, and Maya Nadare. "Molecular Characterization of Trichoderma Mutants with RAPD Marker." International Journal of Current Microbiology and Applied Sciences 7, no. 10 (2018): 2799–806. http://dx.doi.org/10.20546/ijcmas.2018.710.325.

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Gawel, N. J., and A. C. Bartlett. "Characterization of differences between whiteflies using RAPD-PCR." Insect Molecular Biology 2, no. 1 (1993): 33–38. http://dx.doi.org/10.1111/j.1365-2583.1993.tb00123.x.

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Jalawadi, S., V. C. Kanamadi, H. R. Basavarajappa, G. Prabhuling, G. S. K. Swamy, and R. B. Mukesh Chavan. "MOLECULAR CHARACTERIZATION OF SAPOTA GENOTYPES USING RAPD MARKERS." Acta Horticulturae, no. 1024 (March 2014): 61–67. http://dx.doi.org/10.17660/actahortic.2014.1024.4.

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Schmidt, Olaf, and Ute Moreth. "Characterization of Indoor Rot Fungi by RAPD Analysis." Holzforschung 52, no. 3 (1998): 229–33. http://dx.doi.org/10.1515/hfsg.1998.52.3.229.

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Sweety, MA, MM Hossain, MA Hoque, et al. "Molecular Characterization of Tropical Strawberry Genotypes." Plant Tissue Culture and Biotechnology 27, no. 1 (2017): 33–39. http://dx.doi.org/10.3329/ptcb.v27i1.35010.

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Genetic variability of tropical strawberry genotypes was determined through Ramdom Amplified Polymorphic DNA (RAPD) primers. Out of 14 RAPD primers six showed reproducible and polymorphic bands. The results revealed that the maximum polymorphic bands were produced by the primer OPB 12. Ten genotypes were differentiated into two clusters on the basis of Unweighted Pair Group Method With Arithmetic Averages (UPGMA). Genotypic variation based on molecular characterization indicated that genotypes belonging to two different clusters depend on their genetic component. So, selection of parents from

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Dey, Bhaskar, Ashok Verma, Salil Tewari, and Ashutosh Dubey. "Biochemical and Molecular Characterization of Bamboo Variants." Journal of Non Timber Forest Products 27, no. 1 (2020): 50–55. http://dx.doi.org/10.54207/bsmps2000-2020-hqnn97.

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Classical taxonomic studies of the bamboos are based on floral morphology and growth habit, which can cause problems in identification due to erratic flowering. Identification and genetic relationships in 18 species of bamboo were investigated using SDS-PAGE and RAPD analysis. Protein profiles of 18 bamboo species revealed clear polymorphism which can be used to identify species. Dendrogram based on SDS-PAGE data separated 18 species into two major clusters with Bambusa nutans & B. polymorpha, Gigantochloa andamanica & Dendrocalamus longispathus, G. andamanica & D. gigangteus showe

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Bogado, Isabel, Adriana Limansky, Emma Sutich, et al. "Molecular Characterization of Methicillin-Resistant Coagulase-Negative Staphylococci from a Neonatal Intensive Care Unit." Infection Control & Hospital Epidemiology 23, no. 8 (2002): 447–51. http://dx.doi.org/10.1086/502083.

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Objective:To evaluate clonal dissemination of methicillin-resistant coagulase-negative staphylococci (CNS).Setting:Neonatal intensive care unit of a 180-bed, university-affiliated general hospital.Patients:Neonates admitted to the neonatal intensive care unit between March 1999 and October 2000, from whom CNS were isolated as a unique pathogen. Patients from other wards from whom epidemiologically unrelated staphylococci strains were obtained served as control-patients.Methods:Conventional methods were used for phenotypic characterization of CNS. Methicillin resistance was determined bymecA po

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Almeida, Álvaro M. R., Fernanda F. Piuga, Silvana R. R. Marin, et al. "Pathogenicity, molecular characterization, and cercosporin content of Brazilian isolates of Cercospora kikuchii." Fitopatologia Brasileira 30, no. 6 (2005): 594–602. http://dx.doi.org/10.1590/s0100-41582005000600005.

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Cercospora kikuchii, involved with the defoliation of soybean (Glycine max) plants, is normally associated with Septoria glycines in late season. Seventy-two isolates from different regions in Brazil, obtained mainly from purple stained seeds, showed phenotypic variation. Cercosporin content and rate of colony growth was higly variable among isolates. A strong correlation between cercosporin content and virulence was identified. Genetic variation among and within populations was evaluated based on 86 RAPD loci. The RAPD analysis clustered all isolates into seven groups. No relationship was obs

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MIR, Javid Iqbal, Nazeer AHMED, Mudasir Hafiz KHAN, et al. "Molecular Characterization of Saffron-Potential Candidates for Crop Improvement." Notulae Scientia Biologicae 7, no. 1 (2015): 81–89. http://dx.doi.org/10.15835/nsb719346.

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In this study, thirty one (31) morphologically distinct selections of saffron crop were used for molecular characterization. Molecular characterization was done through SSR, ISSR and RAPD markers. RAPD and ISSR markers showed significant variation; however, SSR markers did not reveal any variation between the selected clones. The Jaccard’s similarity coefficient ranged from 0.94 to 1.00 with an average of 0.98 among all 31 selections used. Minimum similarity value (0.94) was observed between CITH-S-107 and PAM-S-116 selections. The study provides sufficient knowledge to identify clones with be

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Pawar, A. S., Kunvar Gyanendra Kumar, and R. P. Singh. "Molecular Characterization of Yeast (Saccharomyces cerevisiae) Strains by Using Molecular Marker." International Journal of Horticulture, Agriculture and Food science 8, no. 3 (2024): 57–59. http://dx.doi.org/10.22161/ijhaf.8.3.8.

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Molecular markers reveals polymorphism at the DNA level. Molecular characterization of Yeast (Saccharomyces cerevisiae) Strains with RAPD Molecular markers. The genetic diversity allelic variations among these strains were estimated by using RAPD molecular markers. Twenty Eight RAPD primers were screened out of them eleven primers had shown amplification of which AB1-15, 1283, OPB12, OPO4, SC1 and OPB01 these six primers showed 100% polymorphism. Out of ten wine yeast strains studied at molecular level, two strains showed maximum similarity i.e. 79 % between them viz. NCIM 3045 and 3200. Genet

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Nkongolo, K. K., P. Michael, and W. S. Gratton. "Identification and characterization of RAPD markers inferring genetic relationships among Pine species." Genome 45, no. 1 (2002): 51–58. http://dx.doi.org/10.1139/g01-121.

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Total genomic DNAs were extracted from several populations of pine species and amplified using oligonucleotides of random sequences. Polymorphism in random amplified polymorphic DNA (RAPD) markers was high and sufficient in distinguishing each of the species. Genetic relationships among eight pine species (Pinus sylvestris, Pinus strobus, Pinus rigida, Pinus resinosa, Pinus nigra, Pinus contorta, Pinus monticola, and Pinus banksiana) from different provenances were analyzed. The degree of band sharing was used to evaluate genetic distance between species and to construct a phylogenetic tree. I

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Sánchez-Tuesta, Linda, and Zulita Prieto. "Morphological characterization and band profile of Anastrepha distincta using RAPD-PCR." SCIÉNDO 21, no. 3 (2018): 325–31. http://dx.doi.org/10.17268/sciendo.2018.035.

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Fitzsimons, N. A., T. M. Cogan, S. Condon, and T. Beresford. "Phenotypic and Genotypic Characterization of Non-Starter Lactic Acid Bacteria in Mature Cheddar Cheese." Applied and Environmental Microbiology 65, no. 8 (1999): 3418–26. http://dx.doi.org/10.1128/aem.65.8.3418-3426.1999.

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ABSTRACT Non-starter lactic acid bacteria were isolated from 14 premium-quality and 3 sensorially defective mature Irish Cheddar cheeses, obtained from six manufacturers. From countable plates ofLactobacillus-selective agar, 20 single isolated colonies were randomly picked per cheese. All 331 viable isolates were biochemically characterized as mesophilic (i.e., group II)Lactobacillus spp. Phenotypically, the isolates comprised 96.4% L. paracasei, 2.1% L. plantarum, 0.3%L. curvatus, 0.3% L. brevis, and 0.9% unidentified species. Randomly amplified polymorphic DNA (RAPD) analysis was used to rap

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Cheng, B. F., G. Séguin-Swartz, and D. J. Somers. "Cytogenetic and molecular characterization of intergeneric hybrids between Brassica napus and Orychophragmus violaceus." Genome 45, no. 1 (2002): 110–15. http://dx.doi.org/10.1139/g01-131.

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Twenty-two intergeneric hybrids from a cross between Brassica napus (AACC, 2n = 38) cultivar Oro and the ornamental crucifer Orychophragmus violaceus (OO, 2n = 24) were produced without embryo rescue. The plants were classified into three groups based on morphological and cytological observations and RAPD banding patterns. Plants of Group I had morphological traits of both parents and 2n = 29 chromosomes. In these plants, 62.1% of the pollen mother cells (PMCs) had the pairing configuration 1 III + 9 II + 8 I; the remaining PMCs had 10 II + 9 I. The plants possessed 97.698.8% B. napus specifi

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Deng, Zhanao, Shunyuan Xiao, Shu Huang, and Frederick G. Gmitter Jr. "Development and characterization of SCAR markers linked to the citrus tristeza virus resistance gene from Poncirus trifoliata." Genome 40, no. 5 (1997): 697–704. http://dx.doi.org/10.1139/g97-792.

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Twelve new dominant randomly amplified polymorphic DNA (RAPD) fragments associated with a single dominant gene for resistance to citrus tristeza virus (CTV) were identified using bulked segregant analysis of an intergeneric backcross family. These and eight previously reported RAPDs were mapped in the resistance gene (Ctv) region; the resulting localized linkage map spans about 32 cM, with nine close flanking markers within 2.5 cM of Ctv. Seven of 20 RAPD fragments linked with the resistance gene were cloned and sequenced, and their sequences were used to design longer primers to develop seque

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Sulakvelidze, Alexander, Merab Kekelidze, Tsaro Gomelauri, et al. "Diphtheria in the Republic of Georgia: Use of Molecular Typing Techniques for Characterization of Corynebacterium diphtheriae Strains." Journal of Clinical Microbiology 37, no. 10 (1999): 3265–70. http://dx.doi.org/10.1128/jcm.37.10.3265-3270.1999.

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Sixty-six Corynebacterium diphtheriae strains (62 of the gravis biotype and 4 of the mitis biotype) isolated during the Georgian diphtheria epidemic of 1993 to 1998 and 13 non-GeorgianC. diphtheriae strains (10 Russian and 3 reference isolates) were characterized by (i) biotyping, (ii) toxigenicity testing with the Elek assay and PCR, (iii) the randomly amplified polymorphic DNA (RAPD) technique, and (iv) pulsed-field gel electrophoresis (PFGE). Fifteen selected strains were ribotyped. Six RAPD types and 15 PFGE patterns were identified among all strains examined, and 12 ribotypes were found a

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Carneiro, Andréa Almeida, Eliane Aparecida Gomes, Claudia Teixeira Guimarães, Fernando Tavares Fernandes, Newton Portilho Carneiro, and Ivan Cruz. "Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm." Pesquisa Agropecuária Brasileira 43, no. 4 (2008): 513–20. http://dx.doi.org/10.1590/s0100-204x2008000400010.

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The objective of this work was to evaluate the pathogenicity of 24 Beauveria isolates to Spodoptera frugiperda larvae, and characterize them molecularly through rDNA-ITS sequencing and RAPD markers. Sequencing of rDNA-ITS fragments of 570 bp allowed the identification of isolates as B. bassiana or B. brongniarti by sequence comparison to GenBank. Sixty seven polymorphic RAPD fragments were capable to differentiate 20 among 24 Beauveria isolates, grouping them according to the derived host insect and to pathogenicity against maize fall armyworm larvae. Three RAPD markers were highly associated

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Rocha, Elizângela Almeida, Luciano Vilela Paiva, Humberto Henrique de Carvalho, and Claudia Teixeira Guimarães. "Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers." Crop Breeding and Applied Biotechnology 10, no. 3 (2010): 204–10. http://dx.doi.org/10.1590/s1984-70332010000300004.

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This study aimed to evaluate the genetic diversity and identify potato cultivars by RAPD and SSR markers. The genomic DNA of 16 potato cultivars was amplified with 25 RAPD primers that generated 92 polymorphic bands and 20 SSR primer pairs that produced 136 polymorphic bands. The dendrograms generated by cluster analysis distinguished the cultivars genetically although the dendrograms were not correlated in the comparison of the two markers used. The PIC values demonstrated the high information content of the primers used and 16 potato varieties were identified based on six RAPD primers and th

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TÜTÜNCÜ, Mehmet, Özhan SIMSEK, Yildiz AKA KAÇAR, and Ayzin B. KÜDEN. "Molecular Characterization of Turkish Cactus Pear (Opuntia spp.) by RAPD Markers." Journal of the Professional Association for Cactus Development 18 (December 27, 2016): 65–77. http://dx.doi.org/10.56890/jpacd.v18i.55.

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Opuntia ficus-indica Mill. commonly known as cactus pear is the most agronomicallyimportant species in Cactaceae. Turkey has important genetic resources of Opuntia spp.which should be characterized for further breeding strategies. In this study, molecularcharacterization of plant materials collected from different regions of Turkey in whichOpuntia species grown naturally, was performed by using Random Amplified PolymorphicDNA (RAPD) markers. DNA was successfully amplified by 50 RAPD primers. Among 250bands generated by the RAPD primers, 180 were polymorphic. The number of bandsdetected by a si

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Rodríguez, E., J. Nieto, J. A. Castillo, and T. Gárate. "Characterization of Spanish Trichinella isolates by random amplified polymorphic DNA (RAPD)." Journal of Helminthology 70, no. 4 (1996): 335–43. http://dx.doi.org/10.1017/s0022149x00015637.

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AbstractThe random amplified polymorphic DNA (RAPD) assay was used to find molecular markers able to distinguish Trichinella spiralis from T. britovi, the two recognized Spanish Trichinella species. Fourteen Spanish Trichinella isolates, as well as reference Trichinella isolates representing the five species T. spiralis (T1), T. nativa (T2), T. britovi (T3), T. pseudospiralis (T4) and T. nelsoni (T7) and the three other taxa Trichinella T5, Trichinella T6 and Trichinella T8 of the genus, were characterized by RAPD using both purified and crude DNAs from infective muscle larvae (ML) and seven a

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