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Dissertations / Theses on the topic 'Real-time QPCR'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Allen, Charlotte Annette. "Cytokine detection in EIAV-infected equine monocyte-derived macrophages using quantitative real-time polymerase chain reaction." Thesis, Texas A&M University, 2007. http://hdl.handle.net/1969.1/85887.

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The replication of equine infectious anemia virus (EIAV) in macrophages not only leads to cell death, but also to the induction of a variety of cytokines that may affect immune function. Cytokine production may be responsible for the fever, anorexia, hemorrhages, lethargy or thrombocytopenia seen in the acute and chronic phases of equine infectious anemia (EIA). The study of the equine immune system and inflammatory responses, by measuring cytokine expression, can provide important insight into disease pathogenesis in the horse. We have extended studies of virulent and avirulent EIAV clones by

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2

Pettersson, Erik. "Investigation of tissue factor mRNA levels in human platelets using real-time PCR." Thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-180831.

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Tissue factor (TF), a 47 kDa glycoprotein, is the initiator of the extrinsic pathway of blood coagulation and consequently of the upmost importance when damage to blood vessel occurs. The source of TF in circulation has been investigated. However, the source of TF is still not clear. One theory is that platelets express and increases the expression of TF after stimulation and the aim of our report was to investigate whether platelets really are a source for TF in circulation. Using specific primers for TF mRNA, platelets in plasma from healthy volunteers and from patients suffering from cardia

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3

Patil, Neeraj. "Detection of Sclerotinia sclerotiorum using qPCR assay and comparison between three qPCR systems to check sensitivity." Thesis, Högskolan i Skövde, Institutionen för biovetenskap, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-20265.

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Sclerotinia sclerotiorum is a pathogenic fungus that infects around 400 species of host plants. Stem rot disease caused by this fungus is economically disastrous for Brassica napus cultivators in Sweden. Due to the lack of disease resistant cultivars, disease management has been solely dependent on fungicide application. The current disease prediction models are not scientifically accurate and take into account factors such as weather, previous disease incidence, and conomic effects which often result in unnecessary and excessive use of fungicides by cultivators. Real-Time Polymerase Cha

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Nayak, Arun Kumar. "Stability and quantitative surveillance of Helicobacter pylori and Campylobacter jejuni in environmental waters by real time qPCR." Diss., Connect to online resource - MSU authorized users, 2008.

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5

Amiri, Mehdi. "Real-time PCR detection and PFGE typing of Pseudomonas aeruginosa from cystic fibrosis patients." Doctoral thesis, Università Politecnica delle Marche, 2016. http://hdl.handle.net/11566/243098.

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Pseudomonas aeruginosa (PA) infection is the main cause of mortality in Cystic Fibrosis (CF) patients. Rapid and sensitive PA detection is pivotal to improve the prognosis. This thesis includes two parts. The first focuses on the development of a Real-time PCR protocol to detect dormant- non-culturable PA in sputum samples from CF patients (CFPA). 12 culture-positive (CP) and 29 culture-negative (CN) samples were analyzed by a qPCR targeting ecfX, 24% resulted positive (P), with bacterial counts (102 - 106 cells/ml) also higher than those obtained for CP samples. A significant association of c

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Sharif, Sanaz. "Comparison of real-time PCR assays for screening of meticillin-resistant Staphylococcus aureus." Thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-154460.

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Staphylococcus aureus belongs to the normal flora. Many healthy people are colonized by the bacterium mainly in the nose but also on the skin and on other mucous membranes without showing symptoms. After damage to the skin, the bacterium can enter the wound and cause infections. Methicillin-resistant S. aureus (MRSA) is resistant to b-lactam antibiotics such as penicillin and methicillin. The gene that gives resistance characteristic of MRSA is the mecA-gene. MRSA strains are spread in both hospitals and in the community, and it is important to identify these bacteria with rapid and sensitive

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7

Muradrasoli, Shaman. "Detection and Quantification of Variable Viral RNA by Real-Time PCR Assays." Doctoral thesis, Uppsala universitet, Klinisk virologi, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-9193.

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As the area of nucleic acid based technologies develops, so will our understanding of how structural variations in DNA and RNA pathogens are associated with disease. The overall goal of this thesis is the development of broadly targeted measurement techniques for variable viral RNA by Real-Time PCR (here referred to as quantitative reverse transcriptase PCR, QRT-PCR). In papers I & II, broadly targeted and specific QRT-PCRs were used to study expression of endogenous and exogenous betaretrovirus sequences in human tissues. Results from human tissues demonstrated endogenous betaretrovirus e

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Cao, Cong Yuan. "Real-time qPCR and metagenomics for studying filamentous bulking in full-scale and lab-scale activated sludge treatment systems." Thesis, University of Macau, 2015. http://umaclib3.umac.mo/record=b3335494.

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ALBUQUERQUE, Yvana Maria Maia de. "Reação em cadeia da polimerase em tempo real quantitativa (QPCR) para diagnóstico da tuberculos pulmonar em escarro de pacientes com HIV/aids." Universidade Federal de Pernambuco, 2012. https://repositorio.ufpe.br/handle/123456789/18503.

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Submitted by Caroline Falcao (caroline.rfalcao@ufpe.br) on 2017-04-06T16:49:28Z No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) 2012-tese-YvanaMariaMaiaAlbuquerque.pdf: 1555304 bytes, checksum: ce271c42fa04e7796e60750e627bc8d8 (MD5)<br>Made available in DSpace on 2017-04-06T16:49:28Z (GMT). No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) 2012-tese-YvanaMariaMaiaAlbuquerque.pdf: 1555304 bytes, checksum: ce271c42fa04e7796e60750e627bc8d8 (MD5) Previous issue date: 2012-05-25<br>O diagnóstico da tube

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Pinto, Alessandro. "Real-time aptapcr: a novel approach exploiting nucleic acid aptamers for ultrasensitive detection of analytes for clinical diagnostic and in food analysis." Doctoral thesis, Universitat Rovira i Virgili, 2012. http://hdl.handle.net/10803/80744.

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The thesis aimed to develop and characterize a novel detection approach, which we termed aptaPCR exploiting nucleic acid aptamers as combined recognition and reporter biocomponents for the ultrasensitive detection of analytes. Nucleic acid aptamers are synthetic ligands selected from vast combinatorial libraries through a process referred to as SELEX – Systematic Evolution of Ligand By Exponential Enrichment. As compared to other natural and synthetic receptor, aptamers possess unique chemical and biochemical characteristics, such as: a well known chemistry, remarkable stability, an abi

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Quintero, Silva Yunuen. "Análisis de la expresión génica del metabolismo oxidativo del etanol como indicador de la recuperación de cepas para la elaboración de vinagres." Doctoral thesis, Universitat Rovira i Virgili, 2010. http://hdl.handle.net/10803/8681.

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En la producción de vinagres no se cuenta con cultivos iniciadores en los que se conozca su composición microbiológica y tampoco se conocen las propiedades oxidativas que presentan. La falta de cultivos iniciadores en la producción de vinagres se ve reflejada en una iniciación lenta de la acetificación o en paradas de acetificación. La utilización de inóculos permite, a la vez, la uniformización en la calidad final del producto, asegurando los parámetros de calidad predeterminados. <br/>Las bacterias acéticas son los microorganismos responsables de la biotransformación de vino a vinagre gracia

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Nyarku, Rejoice E. "Development and analytical validation of a genus-specific Brucella real-time PCR assay targeting the 16S-23S rDNA internal transcribed spacer." Diss., University of Pretoria, 2020. http://hdl.handle.net/2263/77428.

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Brucellosis is an economically important bacterial disease of both animals and humans. In sub-Saharan Africa, the diagnosis of the disease remains a challenge. Brucellosis is underreported in South Africa, due to inconsistency in reports of bacteriological and serological tests, which lack adequate sensitivity and specificity in the diagnosis of the disease. They also are ineffective in confirming brucellosis during early stages of the disease. The aim of this study was to develop a 16S-23S ribosomal deoxyribonucleic acid (rDNA) internal transcribed spacer (ITS) quantitative polymerase chain r

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Beukers, Alicia. "Examining antibiotic resistance in the feedlot cattle industry using real-time, quantitative PCR (qPCR) and enterococci as an indicator bacterium." Thesis, The University of Sydney, 2016. http://hdl.handle.net/2123/16106.

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Antibiotics are administered to livestock at subtherapeutic levels to maintain animal health. Many of the antibiotics used are analogues or the same as those used in human medicine, raising the possibility that genes conferring resistance arise within agricultural production systems. This thesis examined antibiotic resistance in the Canadian beef feedlot industry. Real-time, quantitative PCR was used to examine differences in the relative abundance of eighteen resistance genes across five antibiotic families from feedlot cattle faeces and urban environments. The effect of infeed administration

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Mohamed, Nahla. "Molecular Diagnosis of Common Viral Infectious Diseases Based on Real-Time PCR." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7118.

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Setu, Dambhare. "Development of a specific and sensitive method for detection and quantification of Ustilago nuda by qPCR." Thesis, Högskolan i Skövde, Institutionen för biovetenskap, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-20243.

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Loose smut of barley, caused by fungal pathogen Ustilago nuda is one of the major concerns throughout the globe for barley producers. The infection takes place without exhibiting any obvious symptoms and an infected seed lot can only be identified at the heading stage when the fungal teliospores emerge at the place of crop. The percentage losses on yield are directly proportional to the occurrence of infection. Currently available detection methods include seed health testing protocols which are time-consuming and cumbersome. With the globalization of the international market and increased cro

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Dobson, Meredith Lynn. "Assessment of methanotroph presence and activity in dilute vinyl chloride contaminated groundwater." Thesis, University of Iowa, 2011. https://ir.uiowa.edu/etd/2860.

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The extensive use of tetrachloroethene (PCE) and trichloroethene (TCE) as cleaning solvents has resulted in widespread contamination of groundwater systems with vinyl chloride (VC). VC, a known human carcinogen, is primarily formed in groundwater via incomplete anaerobic reductive dechlorination of PCE and TCE. Aerobic, methane-degrading bacteria (methanotrophs), which are capable of VC cometabolism while growing on methane, could be important in natural attenuation of VC plumes that escape anaerobic treatment. Real-time P

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Shahiduzzaman, Md. "Combination of cell culture and quantitative PCR (cc-qPCR) for assessment of efficacy of drugs and disinfectants against Cryptosporidium parvum." Doctoral thesis, Universitätsbibliothek Leipzig, 2010. http://nbn-resolving.de/urn:nbn:de:bsz:15-20100316-075533-2.

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Cryptosporidium parvum is an obligatory intracellular parasitic protist that belongs to the phylum Apicomplexa. Cryptosporidiosis is an infection for which no satisfactory efficient curative treatment is known, especially in immunocompromised individuals. Furthermore, the parasite oocysts show considerable tenacity in the environment. Therefore, new potent drugs along with a simple and reliable experimental model for evaluation of anticryptosporidial measures are urgently needed. The present studies were undertaken to establish a combined cell culture and quantitative PCR assay (cc-qPCR) to as

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Conway, Crystal A. "Study of Secondary Metabolite Gene Expression in Marine Microbial Co-Cultures Using Quantitative Real-Time PCR." NSUWorks, 2010. http://nsuworks.nova.edu/occ_stuetd/222.

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Interactions among microbial organisms often cannot be observed directly, but they can be inferred genetically using new molecular techniques. The analysis of secondary metabolite gene expression produced by co-cultured marine microbial species allows us to see how these organisms interact with one another when kept in the same environment. Co-cultures of three different strains of marine bacteria, P. aeruginosa PAO1, Roseobacter denitrificans OCH114, and Salinispora arenicola CNS-205 were grown in a laboratory setting, and using the Real-Time qPCR method gene expression levels of two differen

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Harshman, D. K., B. M. Rao, J. E. McLain, G. S. Watts, and J. Y. Yoon. "Innovative qPCR using interfacial effects to enable low threshold cycle detection and inhibition relief." AAAS, 2015. http://hdl.handle.net/10150/621255.

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UA Open Access Publishing Fund<br>Molecular diagnostics offers quick access to information but fails to operate at a speed required for clinical decision-making. Our novel methodology, droplet-on-thermocouple silhouette real-time polymerase chain reaction (DOTS qPCR), uses interfacial effects for droplet actuation, inhibition relief, and amplification sensing. DOTS qPCR has sample-to-answer times as short as 3 min 30 s. In infective endocarditis diagnosis, DOTS qPCR demonstrates reproducibility, differentiation of antibiotic susceptibility, subpicogram limit of detection, and thermocycling spe

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Eulálio, Inês Mariana Cardoso. "Developmente of a CNVs detection method through qPCR." Master's thesis, Universidade de Aveiro, 2017. http://hdl.handle.net/10773/22507.

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Mestrado em Biotecnologia<br>Os copy number variations (CNVs) consistem em segmentos de DNA de uma kilobase ou mais, que se encontram num número variável de cópias, em comparação com um genoma de referência. A deteção de CNVs é convencionalmente realizada através de técnicas de citogenética, como fluorescence in situ hybridization e array comparative genomic hybridization, ou com base em PCR, como multiplex ligation-dependent probe amplification, SNP arrays ou deep sequencing. Porém, a evolução da técnica de PCR quantitativo em tempo real (qPCR) permitiu que fosse, actualmente, considerada o m

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Segal, Jonathan. "Real-time qPCR Assay Development for Detection of Bacillus thuringiensis and Serratia marcescens DNA and the Influence of Complex Microbial Community DNA on Assay Sensitivity." FIU Digital Commons, 2013. http://digitalcommons.fiu.edu/etd/1030.

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Real-time quantitative polymerase chain reaction (real-time qPCR) assays are an effective technique to detect biological warfare agents and surrogate organisms. In my study, primers were designed to detect chromosomal DNA of biological warfare agent surrogates B. thuringiensis and S. marcescens (representing B. anthracis and Y. pestis, respectively) via real-time qPCR. Species-level specificity of the primers was demonstrated through comparisons with a bacterial strain panel and corroborated by qPCR data. Additionally, the primer efficacy was tested when template DNA was spiked into metagenomi

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MELCHIONDA, FILOMENA. "Sviluppo e validazione di un saggio in real-time pcr per la determinazione della quantità e qualità del dna nucleare e mitocondriale umano e le sue applicazioni nelle analisi forensi." Doctoral thesis, Università Politecnica delle Marche, 2021. http://hdl.handle.net/11566/291115.

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La quantificazione del DNA umano ha un ruolo molto importante nella genetica forense. Una stima quanto più accurata della quantità di DNA umano è indispensabile per una pianificazione e un’ottimizzazione delle reazioni di genotipizzazione, così come è altrettanto utile una valutazione della presenza di sostanze inibitrici della reazione di PCR presenti nei campioni forensi. Inoltre, per campioni altamente compromessi, la quantificazione può fornire informazioni sullo stato di degradazione del DNA, indirizzando l'analista forense verso strategie di genotipizzazione più opportune. In questo stu

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Marques, LÃvia Ãrika Carlos. "QuantificaÃÃo por PCR em tempo real de Mycobacterium leprae em amostras de secreÃÃo nasal e biÃpsias de pele em hansenianos." Universidade Federal do CearÃ, 2013. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=9646.

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CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior<br>O Mycobacterium leprae, agente etiolÃgico da hansenÃase, nÃo Ã cultivÃvel em meio axÃnico. A quantificaÃÃo do bacilo realizada pelo exame baciloscÃpico e histopatolÃgico Ã Ãtil para a classificaÃÃo da hansenÃase na escolha e monitoramento do tratamento. No entanto, esta metodologia produz resultados de especificidade e sensibilidade limitada. A PCR em tempo real (qPCR) Ã um ensaio sensÃvel e especÃfico que permite a quantificaÃÃo a partir de diversas amostras e pode ser utilizada no diagnÃstico diferencial de muitos patÃgenos. AtÃ

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Rosa, Fernanda Borowsky da. "A sensibilidade em cavidade oral e orofaríngea e pesquisa do Mycobacterium leprae na saliva de pacientes com Hanseníase." Universidade Federal do Amazonas, 2011. http://tede.ufam.edu.br/handle/tede/4670.

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Submitted by Geyciane Santos (geyciane_thamires@hotmail.com) on 2015-10-26T15:08:48Z No. of bitstreams: 1 Dissertação- Fernanda Borowsky da Rosa.pdf: 3044966 bytes, checksum: aae1ab17f7f5348815c8e9c92a04803d (MD5)<br>Approved for entry into archive by Divisão de Documentação/BC Biblioteca Central (ddbc@ufam.edu.br) on 2015-10-26T18:02:55Z (GMT) No. of bitstreams: 1 Dissertação- Fernanda Borowsky da Rosa.pdf: 3044966 bytes, checksum: aae1ab17f7f5348815c8e9c92a04803d (MD5)<br>Approved for entry into archive by Divisão de Documentação/BC Biblioteca Central (ddbc@ufam.edu.br) on 2015-10-26T18:05:0

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Van, Walleghem Elissa. "Analytical performance characteristics and application of diagnostic tests for Namao virus in experimentally infected and wild Manitoba lake sturgeon (Acipenser fulvescens)." Diseases of Aquatic Organisms, 2013. http://hdl.handle.net/1993/30754.

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Namao virus (NV) was associated with mortality in lake sturgeon Acipenser fulvescens reared as part of a conservation stocking program for this endangered species in Manitoba, Canada. The virus itself was large, doubly encapsidated and icosahedral-shaped. Phylogenetic analyses using the major capsid protein showed that NV and other epitheliotropic sturgeon nucleo-cytoplasmic large DNA viruses shared a common evolutionary past and formed a distinct evolutionary lineage within Megavirales. Three PCR tests were developed and their analytical performance was validated for detection of these vir

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Mildenberger, Michael [Verfasser], Tobias [Gutachter] Reuther, and Heike [Gutachter] Walles. "Untersuchung von im Tissue-Engineering-Verfahren hergestellten Oral-Mukosa-Äquivalenten mittels RT-qPCR (reverse transcription quantitative real-time polymerase chain reaction) / Michael Mildenberger ; Gutachter: Tobias Reuther, Heike Walles." Würzburg : Universität Würzburg, 2017. http://d-nb.info/1147682070/34.

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Rocha, Danilo Jobim Passos Gil Da. "DESENHO E VALIDAÇÃO DE UM CONJUNTO DE PRIMERS UNIVERSAIS BACTERIANOS PARA NORMALIZAÇÃO DE ENSAIOS DE PCR QUANTITATIVA EM TEMPO REAL." Instituto de Ciências da Saúde, 2017. http://repositorio.ufba.br/ri/handle/ri/25288.

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Submitted by Hiolanda Rêgo (hiolandarego@gmail.com) on 2018-02-05T14:14:00Z No. of bitstreams: 1 Dissertação_ICS_ ROCHA, D. J. P. G..pdf: 4034030 bytes, checksum: 7085be9f2fda81bc8248196d4b4e6988 (MD5)<br>Made available in DSpace on 2018-02-05T14:14:00Z (GMT). No. of bitstreams: 1 Dissertação_ICS_ ROCHA, D. J. P. G..pdf: 4034030 bytes, checksum: 7085be9f2fda81bc8248196d4b4e6988 (MD5)<br>CNPQ<br>O método mais comum de normalização em ensaios de quantificação relativa da expressão gênica por PCR quantitativa em tempo real (qPCR) faz uso de um gene de referência, que deve manter sua expressão

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Martins, Keller Gabriel. "DETERMINAÇÃO PRÉ-NATAL DO SEXO PELA ANÁLISE DE DNA FETAL LIVRE EM PLASMA MATERNO." Pontifícia Universidade Católica de Goiás, 2017. http://tede2.pucgoias.edu.br:8080/handle/tede/3842.

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Submitted by admin tede (tede@pucgoias.edu.br) on 2017-11-10T16:06:34Z No. of bitstreams: 1 KELLER GABRIEL MARTINS.pdf: 1151760 bytes, checksum: 3a640f73b5ee5ea85d89f69f6ccd9aa9 (MD5)<br>Made available in DSpace on 2017-11-10T16:06:34Z (GMT). No. of bitstreams: 1 KELLER GABRIEL MARTINS.pdf: 1151760 bytes, checksum: 3a640f73b5ee5ea85d89f69f6ccd9aa9 (MD5) Previous issue date: 2017-08-04<br>The determination of fetal sex using maternal plasma is a noninvasive prenatal diagnostic test (NIPDT), offered to pregnant women, especially those with an increased risk of having children with conditions

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Gondard, Mathilde. "A la découverte des agents pathogènes et microorganismes des tiques par séquençage de nouvelle génération et QPCR microfluidique à haut débit." Thesis, Paris Est, 2017. http://www.theses.fr/2017PESC1017.

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Les maladies à transmission vectorielle sont dues à des agents pathogènes transmis par des arthropodes hématophages. Ces vecteurs assurent une transmission active (mécanique ou biologique) d’un agent infectieux d’un vertébré vers un autre vertébré. A l’échelle mondiale, les tiques sont responsables de la transmission de la plus grande variété d’agents pathogènes, elles transmettent des microorganismes responsables de maladies bactériennes (borréliose de Lyme, rickettsioses) ou parasitaires (babésioses, theilérioses), ou même virales (encéphalite à tiques).Les Antilles se situent au cœur de la

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Lowe, Chinn-woan. "A Quadruplex Real-Time PCR Assay for the Rapid Detection and Differentiation of the Burkholderia pseudomallei Complex: B. mallei, B. pseudomallei, and B. thailandensis." BYU ScholarsArchive, 2013. https://scholarsarchive.byu.edu/etd/6247.

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Methods for the rapid detection and differentiation of the Burkholderia pseudomallei complex comprising B. pseudomallei, B. mallei, and B. thailandensis, have been the topic of recent research due to the high degree of phenotypic and genotypic similarities of these species. B. pseudomallei and B. mallei are the causative agents of melioidosis and glanders, respectively. B. pseudomallei and B. mallei are recognized by the CDC as tier 1 select agents. Although B. thailandensis is generally avirulent in mammals, this species displays very similar phenotypic characteristics to that of B. pseudomal

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Davolos, Camila Chiaradia. "Análise da expressão gênica em isolados de Bacillus thuringiensis por PCR tempo real visando o controle de Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) de diferentes populações brasileiras /." Jaboticabal : [s.n.], 2009. http://hdl.handle.net/11449/94947.

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Resumo: Bacillus thuringiensis é entomopatogênica muito utilizada no controle de insetos-praga e a predição de sua atividade tóxica é realizada por PCR na identificação de genes cry. Assim sendo, este trabalho objetivou elaborar novos oligonucleotídeos iniciadores para a detecção das subclasses cry1Ab, cry1Ac, cry1Ca, cry1Ea e cry1Fa, estudar o potencial de controle de 31 isolados de B. thuringiensis à duas populações de Spodoptera frugiperda por meio de bioensaios e analisar a taxa de expressão dos genes presentes nos isolados por qPCR em tempo real. Um par de oligonucleotídeo iniciador foi e

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Bruson, Alice. "Sviluppo di metodi per l'identificazione e l'analisi di sequenze geniche fetali nella circolazione materna per una diagnosi prenatale non invasiva." Doctoral thesis, Università degli studi di Padova, 2012. http://hdl.handle.net/11577/3422437.

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During last decade, molecular biology evolution has lead to a deep knowledge of human genome allowing the in-utero diagnosis of a large number of genetic diseases. Now adays performing a prenatal diagnosis of chromosomal abnormalities and of genetic diseases, requires genetic material from the fetus and this is possible only by invasive procedures such as amniocentesis and chorionic villus sampling. The risk of miscarriage of these technics is between 0.5% and 1% and considering these limits, new alternative and non-invasive screening methods, based on ultrasound and/or biochemical parameters

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Jönsson, Johanna. "Identification of the tick-borne pathogens Anaplasma phagocytophilum, Neoehrlichia mikurensis and Rickettsia in Swedish ticks : Investigation of transovarial transmission and co-infection." Thesis, Linnéuniversitetet, Institutionen för kemi och biomedicin (KOB), 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-53366.

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Globally, vector borne diseases cause more than a million deaths each year and more than a billion infections in humans. Ticks are of big medicinal importance since they can transmit pathogens that can cause serious infections. Some recently discovered pathogens that can cause infections in humans are Anaplasma phagocytophilum (A. phagocytophilum) that can cause human granulocytic anaplasmosis (HGA) and Candidatus Neoehrlichia mikurensis (N. mikurensis) that can cause Neoehrlichiosis. It is still widely unknown how prevalent these pathogens are, if ticks can be infected with both of these path

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Garcés, Sanchez Gabriela [Verfasser], Jean Charles [Akademischer Betreuer] Munch, and Harald [Akademischer Betreuer] Horn. "Quantification of Cryptosporidium parvum and enteroviruses by quantitative Real-Time PCR (qPCR) in environmental samples - Methodological developments for monitoring anaerobic systems / Gabriela Garcés Sanchez. Gutachter: Harald Horn ; Jean Charles Munch. Betreuer: Jean Charles Munch." München : Universitätsbibliothek der TU München, 2013. http://d-nb.info/1044073594/34.

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Davolos, Camila Chiaradia [UNESP]. "Análise da expressão gênica em isolados de Bacillus thuringiensis por PCR tempo real visando o controle de Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) de diferentes populações brasileiras." Universidade Estadual Paulista (UNESP), 2009. http://hdl.handle.net/11449/94947.

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Made available in DSpace on 2014-06-11T19:27:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-07-17Bitstream added on 2014-06-13T18:56:06Z : No. of bitstreams: 1 davolos_cc_me_jabo.pdf: 358910 bytes, checksum: 020446e7d045944b95dfcb371b8ab71e (MD5)<br>Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)<br>Bacillus thuringiensis é entomopatogênica muito utilizada no controle de insetos-praga e a predição de sua atividade tóxica é realizada por PCR na identificação de genes cry. Assim sendo, este trabalho objetivou elaborar novos oligonucleotídeos iniciadores para a detecção

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Modesto, P. "EXPRESSION PROFILE OF IMMUNE RESPONSE GENES IN GOATS WITH EXPERIMENTALLY INDUCED STAPHYLOCOCCUS AUREUS MASTITIS." Doctoral thesis, Università degli Studi di Milano, 2012. http://hdl.handle.net/2434/169565.

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Counteracting infectious diseases of farm animals are an everlasting challenge in food production from livestock and preserving the health of farm animals is highly relevant to maintaining high standards of food quality. Clinical mastitis (CM) is the primary health reason for involuntary culling in dairy small ruminants and causes additional economic losses from costs of veterinary treatments. Complementary strategies are needed, since the classical prophylactic measures sometimes appear too demanding to breeders in terms of time and care, and efficient vaccination against the main pathogens i

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Söderlind, Lina. "Life-history consequenses of host plant choice in the comma butterfly." Doctoral thesis, Stockholms universitet, Zoologiska institutionen, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-68076.

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There is much evidence that herbivory is a key innovation for the tremendous success of insect. In this thesis I have investigated different aspects of host plant utilization and phenotypic plasticity using the polyphagous comma butterfly, Polygonia c-album. Even though external conditions affect a phenotypic plastic response, the outcome is often influenced by a genetic background which may differ among populations. In Paper I we suspected the genetic background to seasonal polymorphism to be X-linked. However, results from interspecific hybridization between two populations suggested that di

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Bonczek, Ondřej. "Izolace DNA z probiotických výrobků s využitím pevných nosičů." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2011. http://www.nusl.cz/ntk/nusl-216691.

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Microbial DNA was isolated from lysed cells of Lactobacillus genus in probiotic products. Reversible adsorption DNA on the surface of carboxyl coated nonporous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) magnetic particles and silicagel coated manganase Perovskite nanoparticles. DNA was adsorbed on the surface of the particles in the presence of 16 % poly(ethylenglycol) (PEG 6000) and 2 M sodium chloride (NaCl) concentrations. The adsorbed DNA was released from particles by low ionic strength TE buffer (pH= 8.0). The quality of isolated DNA was checked by spectr

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Väisänen, Daniel. "Effekten av 10 veckors styrketräning på markörer för hypertrofi, translation och proteolys." Thesis, Gymnastik- och idrottshögskolan, GIH, Institutionen för idrotts- och hälsovetenskap, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:gih:diva-4587.

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Det har forskats mycket på olika signalvägar i det mänskliga genomet, trotts detta finns det många frågetecken som kvarstår. Denna uppsats undersöker några av dem. Syfte: Undersöka förändringar i genuttryck och mRNA-nivåer för hypertrofi- (MRF4) translations- (5.8S & 18S) och proteolysreglerande gener (MuRF1 & GDF-8) efter en 10 veckor lång styrketräningsperiod hos kvinnor och män. Frågeställningar: (1) Finns det en förändring i total mängd RNA före och efter en 10 veckors styrketräningsintervention. (2) Finns det en förändring i uttryck av MRF4, 5.8S, 18S, MuRF1 samt GDF-8 efter en 10

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Okonkwo, Onyinye. "Enhancement of thermophilic dark fermentative hydrogen production and the use of molecular biology methods for bioprocess monitoring." Thesis, Paris Est, 2019. http://www.theses.fr/2019PESC2041.

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Le but de cette thèse était d'améliorer la production thermophile d'hydrogène noir fermenté en utilisant des stratégies microbiennes (bioaugmentation et co-cultures synthétiques) et en améliorant la compréhension de la dynamique de la communauté microbienne, particulièrement dans des conditions de stress telles que des températures fluctuantes et des concentrations élevées du substrat. Afin d'étudier les effets des fluctuations soudaines et à court terme de la température, des cultures de lots incubées initialement à 55 °C (témoin) ont été soumises à des variations de température vers le bas (

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Souza, AlethÃia CarÃzia Baracho de Lima. "Estudos sobre as interaÃÃes das proteÃnas seminais com as cÃlulas espermÃticas e componentes dos diluidores usados na criopreservaÃÃo do sÃmen e sobre marcadores moleculares de parÃmetros do sÃmen em animais de produÃÃo." Universidade Federal do CearÃ, 2014. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=12911.

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FundaÃÃo de Amparo Ã Pesquisa do Estado do CearÃ<br>A tese Ã composta por dois capÃtulos. O primeiro capÃtulo inclui o trabalho cujo objetivo foi investigar o potencial uso de pares de transcritos correlativos baseados em microarranjos como marcadores de fertilidade masculina usando a displasia da bainha fibrosa (DFS) como modelo afetado. Atualmente Ã bastante reconhecido que a tecnologia de microarranjos pode ser limitada pelos custos e que a qualidade dos transcritos permanece relativamente desconhecida. Para responder essas questÃes, nÃs analizamos pares de transcritos estÃveis por qPCR com

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Manasfi, Youssef. "Lutte contre les pathogènes telluriques en contexte horticole : cas du pathosystème Choisya ternata/ Phytophthora spp." Thesis, Normandie, 2017. http://www.theses.fr/2017NORMR082.

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Choisya ternata est une plante ornementale souvent touchée par la maladie de la pourriture racinaire provoquée par Phytophthora. Cette maladie peut induire de pertes allant jusqu’à 80 %, ce qui implique l’utilisation intensive de produits phytosanitaires. Afin de limiter l’utilisation de ces produits toxiques, une meilleure connaissance des acteurs de la défense des plantes est nécessaire. Pour cela, les objectifs de cette thèse sont I) d’identifier les espèces du genre Phytophthora pathogènes de C. ternata, II) d’étudier des acteurs de défense au niveau racinaire et III) de développer une app

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Abrahams, Beynon. "The effects of various combinations of different classes of anticancer drugs and tyrosine kinase inhibitors on the human MCF-7 breast carcinoma cell line." Thesis, University of the Western Cape, 2014. http://hdl.handle.net/11394/3846.

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Magister Scientiae (Medical Bioscience) - MSc(MBS)<br>This study investigated the effects of TKIs on the growth and proliferation of MCF-7 breast carcinoma cells in culture. MCF-7 cells were exposed to different concentrations of TKIs alone and in combination with each other. Inhibition of cell growth by TKIs used individually occurred in a dose- and time-dependent manner. When EGFR Inhibitor I, EGFR Inhibitor II/BIBX1382 and the multi-specific EGFR/ErbB-2/ErB-4 Inhibitor were used in combination with each other at equimolar log dose concentrations, the combined effects on cell growth was sign

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Chen, Yu-Chieh, and 陳羽潔. "Bias of DNA Quantification Caused by Plasmid DNA Conformations in Real-time qPCR Assay." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/54169841758770111012.

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碩士<br>國立臺灣大學<br>微生物與生化學研究所<br>98<br>Real-time quantitative PCR (qPCR) is the “gold standard” method of accurate quantification of genetically modified organisms (GMO). Precise quantification of target gene could be achieved by absolute quantification method based on a standard curve constructed by plasmid DNA. A recent study showed that supercoiled plasmid may suppress PCR amplification in real-time qPCR assay. In this study, we investigate the bias of DNA quantification caused by plasmid DNA conformations (supercoiled, linear, nicked circular and closed circular) both in DNA quantification me

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Rusnáková, Pavlína. "Stanovení exprese mRNA enzymu DHRS3 v lidských tkáních pomocí real-time SYBR green qPCR." Master's thesis, 2015. http://www.nusl.cz/ntk/nusl-332588.

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Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Pavlína Rusnáková Supervisor: RNDr. Lucie Zemanová, PhD. Title of diploma thesis: Determination of mRNA of DHRS3 enzyme in human tissues by real-time SYBR green qPCR Dehydrogenase/reductase (SDR family) member 3 (DHRS3, also known as SDR16C1, retSDR1) is NADPH dependent carbonyl reductase, member of the short-chain dehydrogenases/reductases superfamily. DHRS3 was characterized for the first time by Haeseleer et al. as an enzyme that reduces all-trans-retinal to all-trans-retinol in

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Menino, Sara Patrícia Faria. "Relatórios de Estágio e Monografia intitulada “Parasitoses Intestinais: Diagnóstico recorrendo à técnica de Reação de Polimerização em Cadeia em tempo real quantitativa (qPCR) e multiplex qPCR”." Master's thesis, 2017. http://hdl.handle.net/10316/83695.

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Relatório de Estágio do Mestrado Integrado em Ciências Farmacêuticas apresentado à Faculdade de Farmácia<br>O presente documento apresenta em primeira instância, no âmbito da unidade curricular “Estágio” do Mestrado Integrado em Ciências Farmacêuticas, os relatórios de estágio em Indústria Farmacêutica e em Farmácia Comunitária. O estágio em Indústria Farmacêutica realizou-se nos Laboratórios Expanscience Portugal, empresa farmacêutica localizada em Lisboa, sendo os Assuntos Regulamentares e Qualidade as áreas em que o meu estágio se concentrou. O estágio em Farmácia Comunitária realizou-se na

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Mildenberger, Michael. "Untersuchung von im Tissue-Engineering-Verfahren hergestellten Oral-Mukosa-Äquivalenten mittels RT-qPCR (reverse transcription quantitative real-time polymerase chain reaction)." Doctoral thesis, 2017. https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-155286.

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Im Rahmen dieser Arbeit wurden Fibroblasten und Keratinozyten, welche in vitro auf unterschiedlichen Scaffolds sowohl gemeinsam als auch in Monokulturen gezüchtet wurden, mittels Real-time PCR auf ihre Genausschüttung untersucht, um festzustellen wie sich die Unterlage auf die Genausschüttung auswirkt. Hierzu wurden die Proben sowohl auf die Genexpressionsmarker für die Basallamina Kollagen IV, Laminin 1 und 5 als auch auf die Genexpressionsmarker für die frühe Differenzierung Keratin K13 und K14 untersucht. Als Referenzgen wurde β-Actin ausgewählt, da dieses Gen in den Vorversuchen mit zwei w

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Huang, Han-Ting, and 黃瀚霆. "Establishment a real-time qPCR assay to study the expression of three reproductive-related genes in the oyster drill Thais clavigera." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/5b4p89.

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碩士<br>國立中山大學<br>海洋科學系研究所<br>104<br>Oyster drill (Thais clavigera) is a common intertidal snail in Asia Pacific. It is a widely used bioindicator for monitoring organotin pollution shown as female snail with imposex. However, female skewed sex ratio and reduced penis size in males are observed in Taiwan recently. This phenomenon can’t be explained based on previous morphological and physiological studies. In order to gain better understanding on the abnormality of the oyster drill, the expression of genes in relation to reproduction of aquatic snails including RXR (retinoid X receptor), ER (est

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Song, Zi-Yu, and 宋子裕. "Study on absolute and relative quantification of Taiwan economic and toxic crabs Lophozozymus pictor and Etisus splendidus by Real-Time qPCR." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/4267vr.

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He, Keyu. "The database implementation and algorithm design of qPCR-DAMS a database tool to analyze, manage, and store quantitative real-time PCR data /." 2007. http://digital.library.okstate.edu/etd/umi-okstate-2522.pdf.

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