Academic literature on the topic 'Scaffolding structure'

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Dissertations / Theses on the topic "Scaffolding structure"

1

Uchiyama, Asako. "Structure-Function Relationships in Microviridae External Scaffolding Proteins." Diss., The University of Arizona, 2007. http://hdl.handle.net/10150/195001.

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Microviruses (canonical members: øX174, G4, and alpha3) are T=1 icosahedral virions with a two scaffolding protein-mediated assembly pathway. The external scaffolding protein D mainly mediates the assembly of coat protein pentamers into procapsids. The results of previous genetic studies suggest that helix 1 of D protein may act as a substrate specificity domain, mediating the initial coat-scaffolding protein recognition in a species-specific manner. In an effort to elucidate a more mechanistic model, chimeric external scaffolding proteins were initially constructed in a plasmid, which over-expresses the protein, between the closely related phages G4 and øX174. The results of biochemical and genetic analyses identify coat-scaffolding domains needed to initiate procapsid formation and provide more evidence, albeit indirect, that the pores are the site of DNA entry during the packaging reaction.However, protein concentrations higher than those found in typical infections could drive reactions that may not occur under physiological conditions. In order to elucidate a more detailed mechanistic model, the same chimeric external scaffolding gene was placed directly in the øX174 genome, and the chimeric virus was characterized. The results of the genetic and biochemical analyses indicate that helix 1 most likely mediates the nucleation reaction for the formation of the first assembly intermediate containing the external scaffolding protein. Mutants that can more efficiently use the chimeric scaffolding protein were isolated. These second-site mutations appear to act on a kinetic level, shortening the lag phase before virion production.Finally, by using improved protocols, two novel early assembly intermediates, the 9S* and 12S* particles, have been isolated and characterized. The 9S* particle consists of a coat protein pentamer associated with the internal scaffolding protein. The 12S* intermediate is a complex of a 9S* particle with the major spike protein, and the DNA pilot protein. The existence of internal scaffolding and DNA pilot proteins that were absent in previously characterized intermediates suggest that 9S* and 12S* particles are biologically active intermediates. Moreover, preliminary in vitro assembly experiments performed with the 12S* particle and exogenous external scaffolding protein yield empty capsids-like particle, strongly suggesting that these are the physiologically relevant intermediates.
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2

Malo, Jonathan. "DNA scaffolding : a method for protein structure determination." Thesis, University of Oxford, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.421023.

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3

Enyedy, Albert J. "Robotic Construction Using Intelligent Scaffolding." Digital WPI, 2020. https://digitalcommons.wpi.edu/etd-theses/1356.

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Construction is a complex activity that requires the cooperation of multiple workers. Every year, construction activities cause injuries and casualties. To make construction safer, new solutions could be provided by robotics. Robots could be employed not only to replace human workers, but also to make construction in harsh environments safe and cost-effective, paving the way for enhanced underwater infrastructure, deeper underground mining, and planetary colonization. In this thesis, we focus on the topic of collective construction, which involves the cooperation of multiple robots, by presenting a collective robot construction method of our own. Collective construction can be a more viable option than employing individual, complex robots, by potentially allowing the effective realization of large structures, while offering resilience through redundancy, analogous to insect colonies. Our approach offers a novel solution in the design trade-off between choosing the number of robots involved vs. the complexity of the robots involved. On the one hand, capable and complex robots are expensive, limiting the cost effectiveness of realizing large swarms which provide redundancy and increase the system’s resilience to faults. On the other hand, simple and inexpensive robots can be manufactured in large numbers and offer high redundancy, at the cost of limited individual capa bilities and lower performance. We use two types of robots: intelligent scaffolding and worker robots. The intelligent scaffolding acts as regular scaffolding, allowing the worker robots to navigate the structure they assemble, while also guiding and monitoring the construction of the structure. The worker robots move and connect scaffolding and building material while only knowing the local commands necessary to complete their task. This approach is loosely inspired by termite mounds, in which termites use the process of stigmergy in which they mark construction pellets with pheromones to affect the progress of construction, while navigating the struc ture that they build. Thanks to intelligent scaffolding, construction robots have a simple design that allows minimalist onboard computation and communication equipment. In this thesis, we produced a minimum viable prototype demonstrating this concept. Intelligent scaffolding is realized through smart blocks that can be laid and connected to each other. The smart blocks are capable of simple computation and communication once laid. The construction robot uses local navigation methods by line-following across the scaffolding and building blocks of the system. The blocks and construction robot both have a modular design, simplifying the process of manufacturing and repairs while maintaining a low cost. The robot and blocks use magnets to increase the margin of error during block manipulation and allow for the assembly and removal of scaffolding as well as its reuse between build sites. To communicate with the robot, the intelligent scaffolding blocks send local IR signals, similar to TV remote signals, when the robot is on top of them, minimizing the risk of global interference and keeping the system portable. To monitor the connectivity of the system throughout the life cycle of the structure, electrical connections run through each of the blocks, which indicate the status of the structure and can be used to diagnose the location of breaks in the structure for maintenance.
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4

Dahlberg, Anna. "Translanguaging as a scaffolding structure in a multilingual group studying English in Sweden." Thesis, Högskolan i Gävle, Avdelningen för humaniora, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-23795.

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This study was conducted in order to find out what translanguaging practices are used in an English learning multilingual classroom and how those practices can create scaffolding structures for the students’ language development. By attending a second language English class with adult multilingual students and conducting a structured observation it was possible to achieve gathered material sufficient to answer these questions. In class all the translaguaging incidents were written down and afterwards these incidents were structured into different themes to outline scaffolding structures. After analyzing the notes it was clear to see that in this particular class translanguaging was used as a strategy to develop supportive learning structures. The result shows the translanguaging practices correlation between teacher and students as well as in relation to different types of educational classroom applications. Besides those features the results show some indications about the spoken production of translanguaging.
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5

Baudry, Lyam. "Investigating chromosome dynamics through Hi-C assembly." Electronic Thesis or Diss., Sorbonne université, 2019. http://www.theses.fr/2019SORUS026.

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L'avènement des technologies de séquençage ADN à haut-debit a initié une tendance grandissante dans l'assemblage de génomes. La qualité de ces génomes est un prérequis essentiel pour comprendre les interactions au sein de et entre ces chromosomes. Nos méthodes se basent principalement sur les technologies de capture de conformation de chromosomes comme le Hi-C. Lors d'un protocole de Hi-C, les molécules d'ADN sont réticulées avec les protéines environnantes pour former un complexe protéine-ADN statique et volumineux. Ceci permet de capturer la conformation spatiale en piégeant les molécules physiquement proches dans l'espace. Ainsi, le Hi-C est très approprié pour l'analyse de la structure 3D des génomes, ce qui permet d'obtenir un certain nombre d'informations sur le génome. Il a été ainsi montré que sa structure tridimensionnelle peut être reliée directement à sa structure 1D grâce aux propriétés physiques des polymères d'ADN. De plus, une telle proximité en 3D donne également accès à des informations de compartimentation, ce qui a ouvert la voie à une nouvelle approche de binning métagénomique, connue sous le nom de meta3C. Au cours de ce travail, nous étendons ces méthodes à des études de cas présentant une complexité grandissante. Tout d'abord, nous améliorons les outils d'assemblage de génomes et démontrons leur validité avec l'assemblage de Ectocarpus sp., puis nous mettons en évidence des réarrangements chromosomiques au sein d'assemblages joints de Trichoderma reesei et Cataglyphis hispanica. Enfin, nous utilisons la même approche avec le binning métagénomique sur des échantillons de souris in vivo afin de reconstruire des centaines de génomes<br>The advent of high-throughput DNA sequencing technologies has set off an expanding trend in genome assembling and scaffolding. Such genome quality is an essential preliminary to understand interactions between and among chromosomes. We built upon a computational and technological framework that let us tackle genome assembly problems of increasing complexity. Our methods are mainly based on chromosome conformation capture technologies such as Hi-C. In a Hi-C experiment, DNA molecules are cross-linked with the surrounding proteins and form a large, static protein-DNA complex. This captures the spatial conformation by trapping together molecules that are physically close to each other. Therefore, Hi-C is very suitable for 3D genome structure analysis, which lets us infer a wealth of information about the genome. It was indeed shown that the tridimensional structure of the genome can be unambiguously linked to its 1D structure thanks to the physical properties of DNA polymers. Moreover, such 3D proximity also gives access to cell compartment information, thus opening the way for an additional approach for metagenomic binning, known as meta3C. In this work, we expand upon these methods and apply them to use cases with more and more complexity. We first improve on tools for genome assembly and demonstrate their validity with the scaffolding of Ectocarpus sp., then unveil rearrangements in joint scaffoldings of Trichoderma reesei and Cataglyphis hispanica. Lastly, we use the same approach with metagenomic binning on live mouse microbiome samples to reconstruct hundreds of genomes
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6

De, Rycker Manu. "Characterization of Tankyrase Structure & Function; Evidence for a Role as a Master Scaffolding Protein." University of Cincinnati / OhioLINK, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1102619078.

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7

Taylor, Cynthia Lynn. "Scaffolding the Development of Early Self-Regulation: The Role of Structure and Routine in Children's Daily Activities." PDXScholar, 2011. https://pdxscholar.library.pdx.edu/open_access_etds/287.

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Learning to self-regulate one's behavior is a core developmental task in early childhood. Regulation of behavior is a challenge for young children largely due to cognitive constraints, specifically in the areas of attention and memory. As such, it has been theorized that both caregivers and a child's environment can support the development of behavioral self-regulation by providing cues as to what constitutes acceptable behavior in any given context. Although much research has been conducted on the role caregivers play in this regard, little is known about how a child's environment may also serve to support emerging self-regulation of behavior. The present study sought to identify differences among children's daily activities in terms of their degree of structure and routine and how they related to changes in patterns of self-regulated behavior over time. Twenty-one children ages 6 to 60 months in three age-graded classrooms at a constructivist child-care center were video-taped at three measurement points over a six-month period as they engaged in a variety of daily activities (i.e., free play, meals and clean-up). Trained observers coded for nine self-regulatory behaviors and the daily activities during which they occurred. It was hypothesized that structured and routine daily activities would scaffold the development of self-regulation and internalization such that these behaviors would occur more frequently during activities high in structure and routine. Over time, practice during activities that are high and low in structure and routine should support self-regulated behavior in the absence of structure and routine as well. Overall, results demonstrated that in the presence of environmental cues for expected behavior (i.e., structure and routine) children tend to engage in more self-regulated behavior than in the absence of such cues.
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8

Milojkovic, Biljana. "Factors of safety for standard scaffold structures." Thesis, Oxford Brookes University, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.341679.

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9

Kuriger, Rex J. "Kinematics, statics, and dexterity of planar active scaffolding structures." Ohio : Ohio University, 1997. http://www.ohiolink.edu/etd/view.cgi?ohiou1177095329.

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10

Västerlund, Therese. ""Tänk hur många killar vi hade räddat om vi hade låtit dem läsa faktatext om bilar eller grodor" : En empirisk studie om lärares stöttning och utmaning i läsning av faktatexter i år 4 - 6." Thesis, Linnéuniversitetet, Institutionen för svenska språket (SV), 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-105242.

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Studiens syfte är att undersöka hur lärare från olika skolor arbetar med faktatexter i skolan utifrån ett teoretiskt ramverk som grundar sig i det sociokulturella perspektivet i kombination med Gibbons fyrfältsmodell bestående av olika zoner av lärande där den ultimata zonen för lärande är utvecklingszonen där eleverna får mycket stöttning i kombination med hög kognitiv utmaning. Genom semistrukturerade intervjuer med fem lärare verksamma i årskurserna 4 - 6 kunde studiens data samlas in och datan analyserades sedan utefter frågeställningarna som handlar om hur elever stöttas och utmanas i läsandet av faktatexter. Resultatet visade att lärarna stöttar eleverna på många olika vis, bland annat genom att välja texter kopplade till kontextuella sammanhang samt genom att ha gemensamma läsningar och genomgångar av texter. De utmanar elever på olika sätt, bland annat genom att välja en lite svårare text för att säkerställa att alla elever tar sig igenom en lärandezon. En slutsats är dock att mer fokus läggs på stöttning än på utmaning. Detta stämmer väl överens med tidigare forskning som visat hur elever får stöttning för att bygga upp de olika lässtrategier som behövs för att läsa och förstå en text, men också att det tycks finnas mindre kunskap om hur utmaningen ska gå till. Studiens didaktiska implikationer är att mer fokus bör läggas på hur elever kan utmanas i läsning av faktatexter för att säkerställa att alla elever hamnar i en lärandezon.
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