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Journal articles on the topic 'Spectrophotometric method'

Author: Grafiati

Published: 4 June 2021

Last updated: 26 July 2025

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1

Lestari, Ida, Peni Pujiastuti, and Yari Mukti Wibowo. "Komparasi Metode Titrimetri Dengan Spektrofotometri UV-Vis pada Analisis Chemical Oxygen Demand (COD) Output IPAL Domestik Berdasarkan Linieritas, Akurasi dan Presisi." JURNAL SAINS TEKNOLOGI & LINGKUNGAN 9, no. 4 (2023): 592–602. http://dx.doi.org/10.29303/jstl.v9i4.416.

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The method of a test must be evaluated and tested to ensure that the method is capable of producing valid data. So that validation needs to be done as a quality control that provides assurance that the measurements and results obtained are reliable. This study aims to determine the value and comparison of the values of linearity, accuracy, and precision of the titrimetric and spectrophotometric methods in COD analysis. Determination of the COD value for the titrimetric method was carried out by redox titration using a solution of Ferro Ammonium Sulphate (FAS). While the spectrophotometric meth

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Skripnikova, Tatyana A., Svetlana S. Lysova, Yuri E. Zevatskii, and Tatyana V. Artamonova. "ANALYTICAL WAVELENGTHS IN THE SPECTROPHOTOMETRIC INVESTIGATION OF EQUILIBRIA IN SOLUTIONS." Bulletin of the Saint Petersburg State Institute of Technology (Technical University) 68 (2024): 3–9. http://dx.doi.org/10.36807/1998-9849-2024-68-94-3-9.

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The review discusses approaches to determining the analytical wavelength in the spectrophotometric determination of thermodynamic equilibrium constants in solutions. Particular attention is paid to the use of the traditional spectrophotometric method and the method of concentration UV/Vis spectrophotometry, which has not yet been widely used

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Sandhyarani, K. "Spectrophotometric Method for Analysis of Valasartan." International Journal of Science and Research (IJSR) 10, no. 11 (2021): 660–62. https://doi.org/10.21275/sr211112125122.

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SAAD ANTAKLI, Raghad Kabbani, and And Rama Labban. "Analytical Spectrometric Study For Determining Dapagliflozin Propanediol Monohydrate Individually Or In Presence Of Metformin Hydrochloride In Tablets Formulation." JOURNAL OF ADVANCES IN CHEMISTRY 17 (September 10, 2020): 80–87. http://dx.doi.org/10.24297/jac.v17i.8812.

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First simple spectrophotometric method was developed and applied to determine Dapagliflozin Propanediol Monohydrate by Zero Spectrophotometry and First Derivative Spectrophotometric method for determining of Dapagliflozin Propanediol Monohydrate (DAPA) in the presence of Metformin Hydrochloride (MET). Zero spectrophotometric (ZS) was applied for the determination of (DAPA) at 223.5 nm. Linearity range was (2.61– 31.23) µg/mL. Regression analysis showed a good correlation coefficients R2 = 0.9989. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.569 µg/mL and 1.724 µg

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G., KoteshwarRao* R.VijayaVani M.Shiresha B.Vidya I.Santhosh K. Rajeswar Dutt and K. N. V. Rao. "A NEW METHOD DEVELOPMENT AND VALIDATION OF AXITINIB BULK AND PHARMACEUTICAL DOSAGE FORM BY USINGUV-VISIBLE SPECTROSCOPY AS PER ICH GUIDELINES." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 03 (2018): 1704–10. https://doi.org/10.5281/zenodo.1210043.

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The objective of the present work was to develop a simple, efficient and reproducible spectrophotometric method for the quantitative estimation of Axitinib drug in its active pharmaceutical ingredient (API) form. The developed UV-Visible spectrophotometric method for the quantitative estimation of drug –Axitinib measurement of absorption at a wavelength maximum (λmax) of 260 nm using methanol as diluents. The method was validated as per the ICH guidelines. The proposed method can be successfully applied for the estimation of Axitinib in pharmaceutical dosage forms.The linearity dy

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B. Mahendra, K. Harika Sundari, and T. Vimalakkannan. "Method developed for the determination of apixaban by using U.V. spectrophotometric." International Journal of Research In Pharmaceutical Chemistry and Analysis 1, no. 3 (2019): 83–87. http://dx.doi.org/10.33974/ijrpca.v1i3.115.

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The present work is aim to Develop UV spectrophotometry method for the estimation of Apixaban in its dosage forms. Analysed the marketed formulations for their reliability and accuracy and Performed the recovery studies for the developed UV spectrophotometric method. The developed method was validate for its accuracy precision reproducibility. On the basis of results the UV spectrophotometric method developed for the determination of Apixaban is found to be precise, accurate and cost effective. Hence this method can be used for routine analysis of Apixaban in bulk and pharmaceutical dosage for

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Shirkhedkar, Atul A., and Saurabh B. Ganorkar. "NOVEL ZERO ORDER UV-SPECTROPHOTOMETRIC AND FIRST ORDER DERIVATIVE UV-AUC METHODS FOR ESTIMATION OF ETOMIDATE IMPARTING HYDROTROPIC SOLUBLIZATION." INDIAN DRUGS 52, no. 04 (2015): 15–20. http://dx.doi.org/10.53879/id.52.04.10214.

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Simple, novel UV-spectrophotometric methods have been developed and validated for the estimation of etomidate imparting a hydrotropic solublization phenomenon. Etomidate is an imidazole derivative, approved as a short acting intravenous anesthetic having poor water solubility. As the UV-spectrophotometric estimation was not seen in any published literature, we have reported it here; zero order UV-spectrophotometry (Method I) and First Order Derivative UV-spectrophotometry (Method II) with the application of hydrotropy to counteract the poor water solubility of etomidate. The present research e

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Panda, Sagar S., and Ravi Kumar V.V. Bera. "HPTLC AND FIRST DERIVATIVE UV-SPECTROPHOTOMETRIC METHODS FOR ESTIMATION OF VILAZODONE IN PHARMACEUTICALS." Indian Drugs 60, no. 01 (2023): 61–66. http://dx.doi.org/10.53879/id.60.01.12718.

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A new HPTLC method and a sensitive UV spectrophotometric method were established for estimating vilazodone hydrochloride in its pharmaceutical formulations. An environment-friendly chromatographic mobile phase consisting of ethyl acetate: toluene: formic acid (5:4:1, V/V/V) along with ultraviolet densitometric detection at 240 nm using pre-coated silica gel plates was used. First derivative UV spectrophotometric determinations were performed using methanol as solvent, and amplitudes were measured for quantification purposes. Validation studies were compliant with ICH guidance. Vilazodone shows

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Priya, NAGAM Santhi, P. Siva Krishna, D. Amani, et al. "UV Spectrophotometric Method for Estimation of Voriconazole in Bulk Form." Journal of Drug Delivery and Therapeutics 13, no. 12 (2023): 177–82. http://dx.doi.org/10.22270/jddt.v13i12.6339.

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This study presents a validated UV spectrophotometric method for the precise estimation of voriconazole in bulk formulations. Voriconazole, an essential antifungal agent, demands accurate quantification for its pharmaceutical applications. The proposed method leverages the sensitivity of UV spectroscopy to determine voriconazole concentrations effectively. The method's validation adheres to regulatory guidelines, ensuring reliability and accuracy. Spectrophotometric analysis was performed within a specific wavelength range, demonstrating linearity, precision, accuracy, and robustness acr

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Lei, Yan Hong. "Spectrophotometric Method for the Assay of Aspirin." Applied Mechanics and Materials 602-605 (August 2014): 2097–100. http://dx.doi.org/10.4028/www.scientific.net/amm.602-605.2097.

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A selective method was developed for the determination of aspirin by Spectrophotometric method .The spectra (range: λ=200~320 nm) were processed. The linear ranges of aspirin was at 50.28 ~ 125.7μg • mL-1 .the aspirin showed good linear correlation.The ultraviolet spectrophotometry data of the samples were also used to evaluate the samples’ quantitative composition.It was shown that recovery of the method by standard addition method was respectively valued 102.06% for aspirin .The method was simple,reliable,accurate,reproducible with high sensitivity and selectivity.

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Hauptman, J. G., B. F. Feldman, S. L. O’Neill, A. C. Lippert, and A. T. Davis. "A turbidimetric method for fibronectin assay in the dog." American Journal of Veterinary Research 49, no. 11 (1988): 1935–36. https://doi.org/10.2460/ajvr.1988.49.11.1935.

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SUMMARY An immunoturbidimetric method, using spectrophotometry, for the assay of canine plasma fibronectin concentration was compared with the immunoelectrophoretic method. The spectrophotometric method (S) correlated positively (r = 0.7) and significantly (P < 0.01) with the immunoelectrophoretic method (I). The regression equation was S = 0.37I + 53. Ninety-five percent confidence levels for the regression line were calculated to allow detection, by spectrophotometry, of plasma fibronectin concentrations outside the normal range.

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Weiler, M., J. M. Carrasco, C. Fabricius, and C. Jordi. "Spectrophotometric calibration of low-resolution spectra." Astronomy & Astrophysics 637 (May 2020): A85. http://dx.doi.org/10.1051/0004-6361/201936908.

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Context. Low-resolution spectroscopy is a frequently used technique. Aperture prism spectroscopy in particular is an important tool for large-scale survey observations. The ongoing ESA space mission Gaia is the currently most relevant example. Aims. In this work we analyse the fundamental limitations of the calibration of low-resolution spectrophotometric observations and introduce a calibration method that avoids simplifying assumptions on the smearing effects of the line spread functions. Methods. To this aim, we developed a functional analytic mathematical formulation of the problem of spec

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Rajendraprasad, Nagaraju, Kanakapura Basavaiah, and Kanakapura B. Vinay. "Titrimetric and Spectrophotometric Assay of Oxcarbazepine in Pharmaceuticals Using N-Bromosuccinimide and Bromopyrogallol Red." International Journal of Analytical Chemistry 2011 (2011): 1–8. http://dx.doi.org/10.1155/2011/138628.

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Titrimetric and spectrophotometric methods are described for the determination of oxcarbazepine (OXC) in bulk drug and in tablets. The methods use N-bromosuccinimide (NBS) and bromopyrogallol red (BPR) as reagents. In titrimetry (method A), an acidified solution of OXC is titrated directly with NBS using methyl orange as indicator. Spectrophotometry (method B) involves the addition of known excess of NBS to an acidified solution of OXC followed by the determination of the unreacted NBS by reacting with BPR and measuring the absorbance of the unreacted dye at 460 nm. Titrimetry allows the deter

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Zhekov, Zhivko. "METHOD AND SPECTROPHOTOMETRIC EQUIPMENT FOR WATER RESEARCH." Journal scientific and applied research 5, no. 1 (2014): 70–75. http://dx.doi.org/10.46687/jsar.v5i1.111.

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Modem measurement equipment must feature high measurement precision, swift operation, high sensitivity and reliability. The designing of such equipment includes coordination of the operation of optical, mechanical, and electronic assemblies and units, introduction of microprocessor systems and communication interface equipment etc. Therefore, the design, implementation, calibration etc. requirements for such equipment feature an ever growing variety strictness.

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Sarode, Dhananjay B., Sopan T. Ingle, and Sanjay B. Attarde. "FORMULA ESTABLISHMENT OF COLORLESS Pb(II) COMPLEX WITH N-BENZOYL-N-PHENYL HYDROXYLAMINE (BPA) USING ATOMIC ABSORPTION SPECTROSCOPY." Indonesian Journal of Chemistry 12, no. 1 (2012): 12–19. http://dx.doi.org/10.22146/ijc.21366.

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A new method for determination of stoichiometry of colorless complexes by using atomic absorption spectrophotometric technique in continuous variation method and slope ratio method was described here. This method can be used in same manner as that of mole ratio method and slope ratio method. In this method atomic absorption spectroscopy was used instead of UV-Vis spectrophotometry. Atomic absorption spectrophotometric technique is superior to UV-Vis spectrophotometry as it can be applied to colorless soluble complexes. Pb(II) and n-benzoyl-n-phenyl hydroxylamine react to form colorless complex

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Uncu, Livia, Vladilena Evtodienco, Ecaterina Mazur, Elena Donici, and Vladimir Valica. "Validation of the spectrophotometric method for the dosing of some combined capsule." Moldovan Medical Journal 64, no. 4 (2021): 10–16. http://dx.doi.org/10.52418/moldovan-med-j.64-4.21.02.

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Background: UV-Vis spectrophotometry remains the most accessible spectral method with a high degree of sensitivity and information. The advantage of the method consists in its universality, the ability to combine with other methods, the minimum error, as well as its economic efficiency. The objective of this study was the determination of some validation parameters for the spectrophotometric method of dosing piracetam and nicergoline in combined capsules. Material and methods: Agilent 8453 UV-Vis spectrophotometer, reference standards of piracetam and nicergoline, 0.1 M HCl methanolic solution

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Godambe, R. D., J. I. Disouza, C. M. Jamkhandi, and P. S. Kumbhar. "DEVELOPMENT OF SPECTROPHOTOMETRIC AND FLUOROMETRIC METHODS FOR ESTIMATION OF DARUNAVIR USING QBD APPROACH." International Journal of Current Pharmaceutical Research 10, no. 1 (2018): 13. http://dx.doi.org/10.22159/ijcpr.2018v10i1.24401.

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Objective: The main objective of the present study is to develop newer simple, precise spectrophotometric and fluorometric methods of estimation for Darunavir using coupling agent O-pthaladehyde.Methods: The experimental work was designed for both spectroscopic and fluorometric method development and validation. The method is based on formation complex of Darunavir with O-pthaladehyde. QbD approach was applied by varying different parameters. These parameters were designed into Ishikawa diagram.Results: The complex Darunavir-Phthalaldehyde in methanol with 0.1 N HCl showed linearity for both s

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Antakli, Saad, Leon Nejem, and Monzer Alraii. "Determination of Glibenclamide By Analytical Spectrophotometry." JOURNAL OF ADVANCES IN CHEMISTRY 18 (April 24, 2021): 40–48. http://dx.doi.org/10.24297/jac.v18i.8963.

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Simple and rapid spectrophotometric method was developed and applied to determine Glibenclamide (GB) by zero spectrophotometric method and first derivative spectrophotometric method for determining of (GB) in the presence of Metformin hydrochloride (MET). Zero spectrophotometric (ZS) method was applied for the determination of (GB) at λmax = 300 nm. Linearity range was (4 – 360) μg/mL. Regression analysis showed a good correlation coefficients R2 = 0.99993. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.65 μg/mL and 2.31 μg/mL, respectively. First derivative spectr

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Mazumder, Md Anisur Rahman, and Parichat Hongsprabhas. "Detection of Genistein in Soy Protein Isolate and Soymilk Powder by Spectrophotometric and Chromatographic Method." Journal of Food Science and Technology Nepal 11 (December 31, 2019): 69–73. http://dx.doi.org/10.3126/jfstn.v11i0.16920.

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Genistein proposed as a treatment for osteoporosis for postmenopausal women, elderly men, lowering cardiovascular disease and reduces hormone dependent cancers. Genistein also exerted inhibitory effect on lipid peroxidation induced in vitro by pro-oxidant agents on model and natural membranes on cultured cells and on low density lipoprotein. Genistein detection in soy products is very much important for Food Scientist. Gensitein can be detected by UV-Visible spectrophotometric and HPLC method. This study focused on the detection of genistein by HPLC and spectrophotometric methods. Genistein co

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Lastra, Olga C. "Derivative Spectrophotometric Determination of Nitrate in Plant Tissue." Journal of AOAC INTERNATIONAL 86, no. 6 (2003): 1101–5. http://dx.doi.org/10.1093/jaoac/86.6.1101.

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Abstract A derivative spectrophotometric method was developed to determine NO3– –N in plant tissues. The method is based on measurement of the first-derivative spectrum of nitrosalicylic acid in basic solution. The nitrosalicylic acid was obtained by reaction of samples with salicylic acid in concentrated sulfuric acid and was used by Cataldo et al. in nonderivative spectrophotometry. The main strength of this technique is the lack of matrix background interference, typical of plant extracts in traditional spectrophotometric methods. This method is fast, inexpensive, easy-to-apply, and highly

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Morawski, Roman Z. "Measurement Data Processing in Spectrophotometric Analysers of Food." Metrology and Measurement Systems 19, no. 4 (2012): 623–52. http://dx.doi.org/10.2478/v10178-012-0056-1.

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Abstract Spectrometry, especially spectrophotometry, is getting more and more often the method of choice not only in laboratory analysis of (bio)chemical substances, but also in the off-laboratory identification and testing of physical properties of various products, in particular - of various organic mixtures including food products and ingredients. Specialised spectrophotometers, called spectrophotometric analysers, are designed for such applications. This paper is on the state of the art in the domain of data processing in spectrophotometric analysers of food (including beverages). The foll

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Blazheyevskiy, Mykola Ye, and Yuliia Yu Serdiukova. "Comparison of Three Independent Methods of Cephalexin Determination by Means of Potassium Caroate." Ars Pharmaceutica (Internet) 58, no. 2 (2017): 59–65. http://dx.doi.org/10.30827/ars.v58i2.6380.

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Aims.Three different assays for the quantity determination of Cephalexin in pure substance were developed and compared using potassium caroate as analytical reagent. Materials and Methods. Cephalexin pure substance was used and analytical reaction by means of KHSO5 as an oxidant was studied. Voltammetric, kinetic-spectrophotometric and iodometric methods were developed and validated. Results. The chemical reaction is finished with formation of the S-oxide of Cephalexin in the iodometric and voltammetric methods and the S-oxide hydrolysis product in the kinetic-spectrophotometric method. The re

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Shaker, Yusra M. S. Al, Dawood H. Mohammed, and Hadeel Mustafa Abdalkader. "Spectrophotometric Method for the Determination of Mebendazol in Different Formulation." Pakistan Journal of Medical and Health Sciences 16, no. 4 (2022): 913–15. http://dx.doi.org/10.53350/pjmhs22164913.

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This research presents a new spectrophotometric method used to determine mebendazole using oxidation and coupling reaction. This method is based on using potassium chromate as an oxidizing agent for catechol, which is then combined with imibendazole to form a stable product with brown color. The highest absorption is achieved at 402 nm. Moreover, absorbance coefficient is 1556.0794 l.mol-1.cm-1. Beer's law limits were (4-140) µg/ml. Keywords: Spectrophotometry, mebendazol , Oxidative coupling

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Samuel, J. Bunu Veronica Aniako Varsharani P. Karade Edebi N. Vaikosen Benjamin U. Ebeshi. "Thin-Layer Chromatographic And UV-Spectrophotometric Analysis Of Frequently Utilized Oral Macrolide Antibiotics." International Journal in Pharmaceutical Sciences 1, no. 9 (2023): 265–74. https://doi.org/10.5281/zenodo.8340601.

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Background: Macrolides are bacteriostatic antibiotics used in the management of mild to chronic soft tissues, and upper and lower respiratory and genitourinary tract infections. Misuse of these agents is common among people with infections, thus leading to the development of bacterial resistance.  Also, due to high demand, there likelihood of fake and substandard products in the markets, hence the need to develop simple, cost-effective, and rapid methods for the analysis and percentage determination of these antibiotics. The study aimed to quantify various samples of macrolide using thin-

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Amri, Choirul, Dwi Siswanta, and Mudasir Mudasir. "DETERMINATION OF TRACE NITRITE AS 4-(4-NITROBENZENAZO)- 1-AMINONAPHTHALENE COMPLEX BY EXTRACTION-SPECTROPHOTOMETRY." Indonesian Journal of Chemistry 9, no. 2 (2010): 254–60. http://dx.doi.org/10.22146/ijc.21539.

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A study of extraction-spectrophotometric method for the determination of trace nitrite as 4-(4-nitrobenzenazo)-1-aminonaphthalene complex using n-amylalcohol and chloroform as organic solvents has been done. Results of the study showed that extraction-spectrophotometric determination of nitrite using n-amylalcohol or chloroform was very sensitive and had low limit of detection. Extraction-spectrophotometric method of nitrite using n-amylalcohol gave range of linear concentration 0.000-0.054 mg/L NO2--N, detection limit of 2.09x10-4 mg/L NO2--N, and sensitivity of 34.514 ± 0.398 absorbance unit

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Diroh, Vina Awallina, Rio Gusraya Unaldi, Mutiara Wayu Puspasari, and Muhammad Muzhil Aslam. "NSAID Analysis Using Chromatographic and Spectrophotometric Methods." Asian Journal of Analytical Chemistry 1, no. 1 (2023): 12–17. http://dx.doi.org/10.53866/ajac.v1i1.269.

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The purpose of this review article to evaluate the content of Nonsteroidal anti-inflammatory drugs (NSAID) using chromatographic and spectrophotometric methods. This journal review uses seven analytical methods on NSAID , namely high performance liquid chromatography (HPLC), fourier transform infrared (FTIR), gas chromatography (GC), thin layer chromatography (TLC), and spectrophotometry methods. The outcomes of every method have been elaborated in this paper and all of them can be used to detect the NSAID compounds.

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Bhusari, Sachin, Rukayya Shaikh, and Pravin Wakte Pravin Wakte. "Development and Validation of Uv- Spectrophotometric Method for Estimation of Jatamansone in Nardostachys Jatamansi." International Journal of Pharmaceutical Research and Applications 10, no. 2 (2025): 220–28. https://doi.org/10.35629/4494-1002220228.

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Aim: This study aimed to develop and validate a simple, precise, and cost-effective UV- visible spectrophotometric method for the estimation of jatamansone in Nardostachys jatamansi extract. The analysis parameters were selected following the ICH Q2 (R1) guideline. Methods: Jatamansone solution was scanned across the UV-visible range to identify its wavelength of maximum absorbance. Calibration standards of jatamansone were prepared, and a concentration vs. absorbance calibration curve was plotted. The developed UV-visible spectrophotometric method was utilized to estimate jatamansone in stand

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R, Selvi S. "UV-Visible Spectrophotometric Method of Paracetamol Tablet Formulation." International Journal of Trend in Scientific Research and Development Volume-2, Issue-5 (2018): 1749–53. http://dx.doi.org/10.31142/ijtsrd17154.

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Atole, Dipali M., and Hrishikesh H. Rajput. "ULTRAVIOLET SPECTROSCOPY AND ITS PHARMACEUTICAL APPLICATIONS- A BRIEF REVIEW." Asian Journal of Pharmaceutical and Clinical Research 11, no. 2 (2018): 59. http://dx.doi.org/10.22159/ajpcr.2018.v11i2.21361.

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Rapid and easy analytical methods are needed due to increasing number of multicomponent formulations, biotherapeutic products and samples of complex matrix in que. Number of Ultraviolet (UV) spectrophotometric methods used for these purpose. Different types of UV spectrometric methods developed on the basis of principle of additivity, absorbance difference, processing absorption spectra. The aim of this review is to present information on simultaneous equation method, difference spectrophotometry, derivative spectrophotometry, absorbance ratio spectra, derivative ratio spectra, successive rati

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Ivanovna, Kucherenko Lyudmila, Parniuk Natalia Viktorovna та Khromylova Olga Vladimirovna. "VALIDATION OF THE QUANTITATION METHODS OF 1-(β-PHENYLETHYL)-4-AMINO-1,2,4- TRIAZOLE BROMIDE SUBSTANCE BY SPECTROPHOTOMETRIC METHOD". Asian Journal of Pharmaceutical and Clinical Research 11, № 2 (2018): 231. http://dx.doi.org/10.22159/ajpcr.2018.v11i2.22740.

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Objective: The aim of the work was to determine the validation characteristics for the developed method of quantitation of 1-(β-phenylethyl)-4- amino-1,2,4-triazole bromide substance by absorption spectrophotometry in the ultraviolet region (UV).Methods: For study purposes, the 1-(β-phenylethyl)-4-amino-1,2,4-triazole bromide substance and the standard sample, obtained by the state enterprise “plant of chemical reagents” of scientific and technological complex “institute of single crystals” of the National Academy of Sciences of Ukraine, was used. In the course of our work, we used chemical, p

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E Souza, marinês J., Natália Canedo, Paulo S. Souza Filho, and Ana M. Bergold. "Development of an Ultraviolet Spectrophotometric Method for the Determination of Ceftiofur Sodium Powder." Journal of AOAC INTERNATIONAL 92, no. 6 (2009): 1673–80. http://dx.doi.org/10.1093/jaoac/92.6.1673.

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Abstract A UV spectrophotometric method was developed for determination of ceftiofur sodium in the drug substance and sterile powder for injection. The method validation, which yielded good results, included evaluation of the range, linearity, intraand interday precision, accuracy, recovery, specificity, robustness, LOQ, and LOD. The UV spectrophotometric determinations were performed at 292 nm. Good linearity was obtained between 2.5 and 20.0 g/mL. A prospective validation showed that the method is linear (r = 0.9999) and precise, with RSD values of 0.3 for product A and 0.4 for product B. Th

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Omotoso, E. A., and E. B. Unaji. "Comparative evaluation of some moxifloxacin hydrochloride tablet brands marketed in Nigeria using five different validated analytical methods." Nigerian Journal of Pharmaceutical Research 19, no. 1 (2024): 109–19. http://dx.doi.org/10.4314/njpr.v19i1.11s.

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Background: Assay of pharmaceuticals is an important aspect of quality control. It is necessary to compare the bioequivalence of generic brands of the any drug to an innovator/comparator brand as this forms the basis for comparing their therapeutic equivalence.Objective: This study aimed to determine the most accurate method for the assay of moxifloxacin hydrochloride (MOX-HCl) tablet brands in Nigerian markets by using five different validated analytical methods and also verify their interchangeability.Material & Methods: This study involved three brands of MOX-HCl including the comparato

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Marques, Morgana Souza, Fernando Dal Pont Morisso, Fernanda Poletto, and Irene Clemes Külkamp Guerreiro. "Development of derivative spectrophotometric method for simultaneous determination of pyrazinamide and rifampicin in cubosome formulation." Drug Analytical Research 5, no. 1 (2021): 46–50. http://dx.doi.org/10.22456/2527-2616.111454.

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The ultraviolet spectrophotometry analysis for quantitative assay of drugs is a method accurate, sensitive, selective and reproductive with the advantage of being a simple and less expensive method. In this study, a derivative ultraviolet spectrophotometric method was developed for simultaneous determination of pyrazinamide (PYZ) and rifampicin (RIF). The spectrophotometric method was evaluated according to validation guidelines for specificity, linearity, limits of detection and quantification, precision, accuracy and robustness. The first-derivative spectra were obtained and by the zerocross

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Vershinina, Yulia S., Ilya V. Mitin, Andrey V. Garmay, Gleb K. Sugakov, and Irina A. Veselova. "Simple and Robust Approach for Determination of Total Protein Content in Plant Samples." Foods 14, no. 3 (2025): 358. https://doi.org/10.3390/foods14030358.

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The determination of total protein in plant samples is a difficult task, as classical nitrogen-based methods are not selective for the nature of nitrogen, and the results of biochemical methods are influenced by both associated compounds and the complex composition of the protein matrix. Using electrophoretic separation of three commercial sunflower protein samples, it was determined that the studied proteins are a mixture of salt-soluble globulins and water-soluble albumins of different molecular weights. The total protein content of the studied samples was determined using five spectrophotom

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N., Mohana Vamsi 1. *. Dr. Y. Padmanabha Reddy 2. Dr. R. Vijay Kumar 3. "DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF EMTRICITABINE AND TENOFOVIR IN BULK AND DOSAGE FORM." Journal of Scientific Research in Pharmacy 7, no. 6 (2018): 56–63. https://doi.org/10.5281/zenodo.1286317.

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<strong><em>ABSTRACT</em></strong> <strong><em>S</em></strong><em>imple, rapid, sensitive, precise and specific UV spectrophotometric method for the determination of Emtricitabine (EMB) and Tenofovir (TEN) in bulk drug and pharmaceutical dosage form was developed and validated<strong>. </strong>A simple UV spectrophotometric method has been developed and validated with different parameters such as linearity, precision, repeatability, limit of detection (LOD), Limit of Quantification (LOQ), accuracy as per ICH guidelines. The method was found to be linear in the range of 25-150% for Tenofovir a

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Zuijderhoudt, F. M. J., J. Dorresteijn-De Bok, and K. Te Velde. "Evaluation of a First-Line Spectrophotometric Screening Test for Increased Urine Porphyrin Excretion." Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 32, no. 2 (1995): 186–89. http://dx.doi.org/10.1177/000456329503200209.

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We compared a spectrophotometric screening test for urine porphyrin concentration with a high performance liquid chromatography (HPLC) method. The screening test gave lower values than those obtained by HPLC, but the overall correlation was good. Occasionally, spectrophotometry failed to detect porphyrins in the urine which were detected by HPLC. The type of porphyria had no influence on the efficacy of the screening method. Receiver operating characteristic plot analysis of the screening test led to a cut-off value of 110 nmol/24 h with a sensitivity of 96% and a specificity of 86%. We conclu

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S. Panda, Sagar, Ravi Kumar V. V. Bera, and Biswajit Sahu. "APPLICATION OF ICH Q14 CONCEPT FOR CHEMOMETRICS-ASSISTED DEVELOPMENT OF SPECTROPHOTOMETRIC METHODS FOR ESTIMATING PIMAVANSERIN IN TABLETS." Indian Drugs 59, no. 09 (2022): 74–81. http://dx.doi.org/10.53879/id.59.09.12191.

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In the present study, a novel analytical procedure development concept is utilized along with a chemometrics approach. Two sensitive UV spectrophotometric methods were developed for quantifying a new antipsychotic agent, pimavanserin tartarate, in tablets. The scanning speed and sampling interval of the UV spectrophotometric methods were the critical method variables investigated and optimized by a face-centred cubic design during the development phase. A zero-order (222 nm) and a first derivative (maxima: 256 nm and minima: 283 nm) method displayed linear response over 0.5-100 µg mL-1, with i

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Amol, Amrutkar, Aher Smita, and Bachhav Rishikesh. "Development And Validation Of UV-HPLC Method For The Estimation Of Diacerein In Bulk And Tablet Dosage Form." International Journal In Pharmaceutical Sciences 1, no. 1 (2022): 94–103. https://doi.org/10.5281/zenodo.6220056.

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<strong>Objective: </strong>Objective of the present analytical research work was to develop and validate Spectrophotometric method and High Performance Liquid Chromatographic method (HPLC Method) for the Diacerein bulk and tablet dosage form <strong>Methods: </strong>A spectrophotometric method and a HPLC method have been developed and validated for estimation of Diacerein in bulk. <strong>Method A (UV SPECTROMETRY Method): </strong>Methanol was used for the preparation of stock and working standard solutions of the drugs. 400-200nm UV range was used to scanned standard solutions of drugs usi

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Dr.L.Satyanarayana*, and T.Padmini. "SPECTROPHOTOMETRIC DETERMINATION OF AMPRENAVIR IN FORMULATION SAMPLE." Indo American Journal of Pharmaceutical Sciences 04, no. 07 (2017): 2204–8. https://doi.org/10.5281/zenodo.838700.

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A validate rapid, economical and sensitive visible spectrophotometric method has been developed for quantitative determination of amprenavir in bulk drug and tablet samples.This method is validated for irinotecan with chromogenic reagent namely bromo cresol green (BCG) at λ max 417. The calibration curve was linear over a concentration range of 5-40 µg/ml. The relative standard deviation (R.S.D) is less than 1% and average recovery was above 99.60%. Efficient visible spectrophotometric detection at λmax 417nm enabled determination of the drug with no interference from tablet excipients. The pr

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Drozd, J. "Comparison of classic and derivative UV spectrophotometric methods for determination of dextromethorphani hydrobromidum." Acta Facultatis Pharmaceuticae Universitatis Comenianae 59, no. 1 (2012): 22–29. http://dx.doi.org/10.2478/v10219-012-0014-8.

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Comparison of classic and derivative UV spectrophotometric methods for determination of dextromethorphani hydrobromidumA method for the fast determination of dextromethorphani hydrobromidum in pharmaceutical preparations by classic spectrophotometry - zero and first-, second- and third-order derivative spectrophotometry, using "peak - peak" (P - P) and "peak - zero" (P - O) measurements has been performed. The calibration curves are linear within the concentration range of 1.0 - 25.0 μg ml-1 for dextromethorphani hydrobromidum. The procedure is simple, rapid and the results are reliable.

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Vinay, K. B., H. D. Revanasiddappa, Zenita Okram, and Kanakapura Basavaiah. "Permanganometric determination of etamsylate in bulk drug and in tablets." Chemical Industry and Chemical Engineering Quarterly 15, no. 3 (2009): 149–57. http://dx.doi.org/10.2298/ciceq0903149v.

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One titrimetric and two spectrophotometric methods which are simple, selective, sensitive, accurate, precise and economical for the determination of etamsylate (ETM) in bulk drug and in tablets employing permanganate as the oxidimetric reagent are described. In titrimetry, ETM is titrated directly with permanganate in sulphuric acid medium. A direct spectrophotometry (method A) involves treating the aqueous solution of the drug with permanganate in alkaline medium and measuring the bluish green product at 610 nm. In indirect spectrophotometry (method B), the drug solution was treated with a fi

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H.N, Khan* Kodli Puja Sana Javeria MD Zameeruddin A. G. Mangulkar V.B Bharkad. "STABITY INDICATING DISSOLUTION METHOD DEVELOPMENT FOR ESTIMATION OF METHYLDOPA AND HYDROCHLOROTHIAZIDE IN COMBINE DOSAGE FORM." Indo American Journal of Pharmaceutical Sciences 04, no. 09 (2017): 3024–32. https://doi.org/10.5281/zenodo.897504.

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The aim of this work was to develop validate a dissolution test for mthyldopa and Hydrochlorothiazide in combination tablets using spectrophotometric method. The dissolution established conditions were 900 mL of 0.1M HCl pH 1.0 as dissolution medium, using a paddle apparatus at a stirring rate of 50 rpm. The drug release was evaluated by UV spectrophotometric method the area of solution were recorded at 274-284 nm and266-276 nm for MD and HCTZ respectively . it can be concluded that the method developed consists in an efficient alternative for assay of dissolution for tablets. The method was v

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H.N, Khan* Kodli Puja Sana Javeria MD Zameeruddin A. G. Mangulkar V.B Bharkad. "STABITY INDICATING DISSOLUTION METHOD DEVELOPMENT FOR ESTIMATION OF METHYLDOPA AND HYDROCHLOROTHIAZIDE IN COMBINE DOSAGE FORM." Indo American Journal of Pharmaceutical Sciences 04, no. 09 (2017): 3024–32. https://doi.org/10.5281/zenodo.910187.

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The aim of this work was to develop validate a dissolution test for Methyldopa and Hydrochlorothiazide in combination tablets using spectrophotometric method. The dissolution established conditions were 900 mL of 0.1M HCl pH 1.0 as dissolution medium, using a paddle apparatus at a stirring rate of 50 rpm. The drug release was evaluated by UV spectrophotometric method the areas of solution were recorded at 274-284 nm and266-276 nm for Methyldopa and Hydrochlorothiazide respectively. It can be concluded that the method developed consists in an efficient alternative for assay of dissolution for t

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C., H. Raghavan Nambiar, Narayana B., V. Sreekumar N., A. Nazareth R., and Gopalakrishna Bhat N. "Spectrophotometric determination of platinum(IV) using anthranilic acid." Journal of Indian Chemical Society Vol. 79, Sep 2002 (2002): 778–79. https://doi.org/10.5281/zenodo.5844661.

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Department of Studies in Chemistry, Mangalore University, Mangalagangotri-574 199, India <em>E-mail : </em>nbadiadka@yahoo.co.uk         <em>Fax : </em>91-824-287367 <em>Manuscript received 11 July 2001, revised 22 January 2002, accepted 16 March 2002</em> A rapid and sensitive spectrophotometric method for the determination of Pt<sup>IV</sup> with anthranilic acid is described. Anthranilic acid reacts with Pt<sup>IV</sup> at pH 3.0 to give a pink coloured complex, which shows λ<sub>max</sub> at 372 nm. Sandell's sensitivity and molar absorptivity of the com

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Mostafa, Nadia M., Laila Abdel-Fattah, Soheir A. Weshahy, Nagiba Y. Hassan, and Shereen A. Boltia. "Validated Stability-Indicating Spectrophotometric Methods for the Determination of Cefixime Trihydrate in the Presence of its Acid and Alkali Degradation Products." Journal of AOAC INTERNATIONAL 98, no. 1 (2015): 35–45. http://dx.doi.org/10.5740/jaoacint.14-074.

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Abstract Five simple, accurate, precise, and economical spectrophotometric methods have been developed for thedetermination of cefixime trihydrate (CFX) in the presence of its acid and alkali degradation products without prior separation. In the first method, secondderivative (2D) and first derivative (1D) spectrophotometry was applied to the absorption spectra of CFX and its acid (2D) or alkali (1D) degradation products by measuring the amplitude at 289 and 308 nm, respectively. The second method was a first derivative (1DD) ratio spectrophotometric method where the peak amplitudes were measu

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Dagdu, Kedar Vikas, Manoj Gadhave, Shubham Bhujbal, and Bhushan Shrinath. "Area Under Curve by UV Spectrophotometric Method for Determination Albendazole in Bulk." Journal of Drug Delivery and Therapeutics 9, no. 6 (2019): 47–50. http://dx.doi.org/10.22270/jddt.v9i6.3667.

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The aim of present investigation is to establish simple, precise, and rapid Spectrophotometric method for the quantification of Albendazole in Active Pharmaceutical Ingredient. In this, work is carried out to for estimation of Albendazole bulk by utilizing an Area under Curve (AUC) method using UV – Visible Spectrophotometry. The study is designed to validate the developed methods as per ICH guidelines. For this purpose the wavelength range between 200-400 nm was selected. Methanolic distilled water (50 ml methanol used for stock solution and serial dilution in 25 ml distilled water) was used

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Larisa, Alagić-Džambić, Gjuraj Mergime, Dacić Minela, Hukić Minela, and Džambić Mirsad. "UV VIS method of estimation for Glimepiride in Tablets." Chemistry Research Journal 8, no. 6 (2023): 1–4. https://doi.org/10.5281/zenodo.11390450.

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<strong>Abstract </strong> A simple spectrophotometric method was developed and validated for determination of Glimepiride in pharmaceutical products. The method was based using 0.1 mol / L sodium hydroxide as solvent. The absorbance maximum was at 235 nm, 233 nm, and 237 nm. The correlation coefficients on three absorbance point were r=0.9989. The method was validated, and results obtained for the assay of five different brands of Glimepiride tablets. The proposed method was successfully applied to the spectrophotometric determination Glimepiride.

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CH., V. S. Gautam* N. Harika V. Balaji V. Srinivas Prasad. "METHOD DEVELOPMENT AND VALIDATION OF DACLATASVIR IN BULK & PHARMACEUTICAL DOSAGE FORM BY UV-VISIBLE SPECTROPHOTOMETRY." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 03 (2018): 1980–85. https://doi.org/10.5281/zenodo.1213232.

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Objective: The objective of the present work is to develop a simple, efficient, and reproducible spectrophotometric method for the quantitative estimation of hepatitis-C drug - Daclatasvir in active pharmaceutical ingredient(API) form and in pharmaceutical dosage form Methods: The developed ultraviolet spectrophotometric method for the quantitative estimation of hepatitis-C drugs - Daclatasvir based on measurement of absorption at a wavelength maximum (λmax) of 317 nm using methanol as solvent. Results: The method was validated in terms of, precision, linearity, accuracy, and robustness

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Blazheyevskiy, Mykola, and Lyubomyr Kryskiw. "Kinetic Spectrophotometric Method for the Determination of Suxamethonium Chloride." Chemistry & Chemical Technology 9, no. 3 (2015): 261–65. http://dx.doi.org/10.23939/chcht09.03.261.

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Turak, Fatma, and Mahmure Ustun Ozgur. "Simultaneous Determination of Allura Red and Ponceau 4R in Drinks with the Use of Four Derivative Spectrophotometric Methods and Comparison with High-Performance Liquid Chromatography." Journal of AOAC INTERNATIONAL 96, no. 6 (2013): 1377–86. http://dx.doi.org/10.5740/jaoacint.12-393.

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Abstract Four simple, rapid, sensitive, and accurate spectrophotometric methods were developed for the simultaneous determination of Allura Red (AR) and Ponceau 4R (P) without previous chemical separation. The first method, derivative spectrophotometry, depends on first derivative spectrophotometry with zero-crossing and peak-to-base measurement. The second method, derivative ratio spectrophotometry, uses the first derivative of the ratio spectra. The ratio spectra are obtained by dividing the absorption spectra of a binary mixture by that of one of the components. The third method, differenti

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