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Contreras-Dominguez, Monica. "Dégradation de tanins condensés par des champignons filamenteux : analyse structurelle et enzymatique." Aix-Marseille 1, 2007. http://www.theses.fr/2007AIX11035.
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Les tanins condensés (TC) sont présents dans de nombreux produits agro-industriels et sont connus pour leurs propriétés anti-nutritionnelles. Ces propriétés peuvent être modulées par des enzymes fongiques. Après criblage, Aspergillus fumigatus MC8 a été retenue pour sa capacité à croître et dégrader 30 % des procyanidines de pomme purifiés qui possèdent un degré de polymérisation moyen de 8 (DP). Le DP augmente de 8 à 15 au cours de la fermentation, coincidant avec une diminution des oligomères de procyanidine. Le pool enzymatique extracellulaire d'Aspergillus fumigatus est capable de dégrader deux dimères, la procyanidine B2 et la procyanidine B1 à un pH entre 6 et 8. Le produit de dégradation principal de la PB2 (PB2-X) a un temps de rétension de 10,5 minutes et une masse m/z de 609. La chromatographie haute pression couplée à la spectrométrie de masse (HPLC/MSn) a permis d'étudier la structure de PB2-X. Egalement, une analyse structurelle de PB2-X a été faite suite à une thiolyse acide. Douze fragments ioniques m/z 565, 547, 457, 439 (deux fragments d'ions) 421, 413, 377, 395, 351, 287 et 277 ont été complètement identifiés. Il en a été déduit qu'une oxygénase d'Aspergillus fumigatus avait modifié l'unité terminale de PB2 sans modifier l'unité d'extension. Une analyse par spectrométrie infrarouge a permis de confirmer la présence d'une lactone au niveau de l'unité terminale. Les mêmes études ont été faites sur PB1 indiquant que dans ce cas, l'unité d'extension était modifiée et non l'unité terminale. Enfin, les conditions de production et de purification de l'enzyme impliquée ont été optimisés
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Engozogho, Anris Starlin Peguy. "Etude des produits connexes de la transformation industrielle du bois d’Okoumé du Gabon : analyse et mise en œuvre de nouveaux matériaux." Thesis, Pau, 2020. http://www.theses.fr/2020PAUU3005.
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Ce travail de thèse s’est organisé autour de la valorisation des produits connexes issus de la première et de la deuxième transformation des bois du Bassin du Congo en général et de l’Aucoumea Klaineana Pierre (Okoumé) du Gabon en particulier.En effet, 85% des produits connexes issus de cette industrie, sont incinérés 24/24h à ciel ouvert, la raison étant l’absence ou les faibles voies de valorisations possibles. Notre objectif premier est de présenter des voies de valorisations capables de réduire considérablement l’incinération de ces rebus. De ce fait et pour répondre à cette première problématique, nous avons procédé dans un premier temps, par une analyse chimique des trois parties du bois (écorce, aubier, cœur), visant à mettre en évidence les différentes molécules et leurs potentiels domaines de valorisation. Les molécules principalement visées étant les polyphénols. Nous avons par la suite porté un accent particulier aux tanins condensés extraits de l’Okoumé, que nous avons caractérisé thermiquement et chimiquement. Afin d’observer leur pouvoir collant, notamment leur capacité à être utilisé comme substrat pour la mise en point d’un adhésif bio-sourcée pour l’industrie du contreplaqué et du panneau.La deuxième voie de valorisation mise en évidence, est la mise au point d’une composite bois-plastique par thermocompression sans passé par une étape d’extrusion préalable. Ce composite pourra trouver son utilisation dans la construction et l’aménagement interneThis thesis work was organized around the promotion of related products from the first and second processing of wood from the Congo Basin in general and Aucoumea Klaineana Pierre (Okoumé) from Gabon in particular.Indeed, 85% of related products from this industry are incinerated 24 hours a day in the open air, the reason being the absence or the weak ways of possible valorizations.Our primary objective is to present ways of recovery capable of considerably reducing the incineration of this waste. Therefore and to answer this first issue, we proceeded first, by a chemical analysis of the three parts of the wood (bark, sapwood, heart), aiming to highlight the different molecules and their potential areas of development. The molecules mainly targeted are polyphenols. We then focused in particular on the condensed tannins extracted from Okoumé, which we characterized thermally and chemically. To observe their stickiness, including their ability to be used as a substrate for the development of a bio-sourced adhesive for the plywood and panel industry.The second recovery method highlighted is the development of a wood-plastic composite by thermocompression without having gone through a prior extrusion step. This composite can find its use in construction and internal design
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Balas, Laurence. "Tanins catéchiques : isolement, hémisynthèse et analyse structurale par RMN 2D homo- et hétéro-nucléaire : contribution à l'étude des tanins du raisin et des vins de Bordeaux." Bordeaux 2, 1992. http://www.theses.fr/1992BOR2B003.
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Brunet, Séverine. "Analyse des mécanismes d'action antiparasitaire de plantes riches en substances polyphénoliques sur les nématodes du tube digestif des ruminants." Toulouse 3, 2008. http://thesesups.ups-tlse.fr/339/.
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Chez les petits ruminants, la maîtrise actuelle du parasitisme par des nématodes gastro-intestinaux repose sur l'emploi d'anthelminthiques de synthèse. En raison du développement de résistances à ces molécules, l'utilisation de plantes riches en tannins condensés est désormais explorée comme solution alternative aux anthelminthiques, mais le mode d'action en reste mal connu. L'objectif de cette thèse était de définir les mécanismes d'action des tannins condensés sur les larves infestantes des trichostrongles digestifs. Nous avons d'abord confirmé que la consommation d'une plante riche en tannins condensés affecte l'installation des larves chez l'hôte, puis montré que les perturbations concernent les deux étapes-clefs de l'installation des larves : le dégainement puis la pénétration dans les muqueuses. La mise en évidence de lésions ultra-structurales et des sites de fixation des tannins condensés chez les larves est venu corroborée les résultats des études fonctionnelles, suggérant un mécanisme d'action direct. L'analyse des principaux facteurs modulant les effets constatés a montré qu'ils sont non-spécifiques de l'espèce de nématode mais varie selon la forme des larves (engainées ou dégainées). Par ailleurs, la concentration et la nature des tannins condensés sont les deux principaux facteurs liés à la plante influençant les propriétés anthelminthiques contre les larves. Des hypothèses expliquant les interactions entre les tannins et les molécules des larves infestantes sont proposéesIn small ruminants, the current control of gastrointestinal parasitism relies on the use of chemical anthelmintics. However, due to the increasing development of resistances to chemical anthelmintics, the potential use of condensed tannin-rich plants represents an alternative solution to the chemical treatments, although the mode of action remains unknown. The objective of the current work was therefore to define the mechanisms of actions of condensed tannins on the infective larvae of gastrointestinal nematodes. First, we have confirmed that the consumption of a condensed tannin-rich plant decreased the establishment of infective larvae in the host. In a second step, it has been shown that both the exsheathment and the penetration of the larvae in the host mucosae were disturbed after contact with condensed tannins. The observations of the ultra-structural modifications and the fixation of condensed tannins on the larvae have confirmed a direct mode of action. In addition, our results have shown that the effects of condensed tannins were non specific of the nematode species but that differences in susceptibility exist between the ensheathed and exsheathed larvae. The effects were also dependent of the concentration and the biochemical nature of the condensed tannins. Finally, hypotheses on the potential interactions at the molecular level between the condensed tannins and the infective larvae are suggested
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Garcia, Renaud. "Développement de résines thermodurcissables et de haute performance à base de tannins : une étude fondamentale." Nancy 1, 1998. http://www.theses.fr/1998NAN10136.
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L’objectif de ce travail était de caractériser par analyse mécanique dynamique (DMA) et résonance paramagnétique électronique (RPE) les réactions de polycondensation et le comportement mécanique d'adhésifs à base de tannins (pin, pécan, quebracho naturel ou fintan) en présence, ou non, de bois et en présence de différents durcisseurs (silice, formaldéhyde, hexamine, urée, diisocyanate alcool furfurylique et leurs mélanges). Dans un premier temps, nous avons cherché à approcher les mécanismes de la polycondensation de résines à base de tannins en présence de bois par DMA. Ces adhésifs naturels comportant différents noyaux phénoliques, nous avons étudié la polycondensation de systèmes modèles à base de formaldéhyde : adhésifs phénol-formaldéhyde et produits phénoliques monomères- formaldéhyde (phénol, pyrogallol, catéchol, phloroglucinol et résorcinol). L’étude du séchage de polymères non réticulables (PEG, amidon, CMC et colle vinylique) a permis d'interpréter les résultats obtenus avec les adhésifs phénoliques synthétiques ou naturels. La DMA en flexion trois points permet en effet de suivre l'évolution des macromolécules d'adhésif en présence de bois : développement linéaire puis tridimensionnel. La DMA permet de déterminer la température de formation du réseau d'enchevêtrement (forces secondaires), la température du début de la formation d'un réseau tridimensionnel (liaisons covalentes), ainsi que la température de gélification de la résine employée et la densité moyenne du réseau final obtenu. Nous nous sommes ensuite intéressés à l'autocondensation naturelle des tannins. La résonance paramagnétique électronique nous a permis de suivre la partie radicalaire de cette réaction, de mesurer le rôle de la réaction radicalaire d'autocondensation par rapport au mécanisme ionique, et d'évaluer l'influence d'additifs sur la partie radicalaire de la réaction de condensation des tannins.
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Prieur-Delorme, Corinne. "Caractérisation chimique des procyanidols de pépins de raisin "Vitis vinifer" : Applications à l'étude des propriétés organoleptiques des vins." Montpellier 2, 1994. http://www.theses.fr/1994MON20209.
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Dans le but d'etablir une relation entre la structure des tanins et leurs proprietes organoleptiques, 5 fractions de procyanidines de pepins de raisin vitis vinifera ont ete isolees. La mise au point d'une methode de thiolyse adaptee a l'analyse des tanins condenses a permis de verifier la validite de notre technique de separation et de preciser la composition des fractions. L'influence du degre de polymerisation et du pourcentage d'unites galloylees sur la capacite d'interaction de ces tanins avec les proteines a ete confirmee. Dans une etude plus generale, l'astringence des vins rouges, determinee par des tests organoleptiques, a ete reliee a leur composition phenolique
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Mirabel, Marie. "Caractéristiques chimiques et organoleptiques des tanins des raisins de Vitis vinifera var. Merlot et Cabernet Sauvignon issus de différents terroirs bordelais." Bordeaux 2, 2000. http://www.theses.fr/2000BOR20794.
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Les tanins jouent un rôle important en œnologie, responsables de la couleur et de la saveur des vins rouges, leur structure chimique est relativement complexe. Nous l'avons étudiée grâce à différentes techniques d'analyse : la thiolyse, la GPC, la CLHP et la RMN. Pour ce faire, nous avons réalisé des étalons de tanins de poids moléculaires distincts, grâce à une précipitation au méthanol/chloroforme. L'application de la thiolyse et de la CLHP en phase normale à l'étude de la composition tannique des raisins (pépins et pellicules) au cours de leur maturation a mis en évidence des phénomènes complexes lors du millésime 1999 sur les terroirs bordelais : diminution des teneurs en tanins de pépins, parallèlement à une polymérisation des tanins ; accumulation des tanins dans les pellicules avec des modifications de structures. L'analyse sensorielle de solutions aqueuses de tanins issus de raisins et de vins nous a permis de définir leur astringence et leur amertume. Ces deux saveurs varient distinctement avec la concentration et la taille des tanins. Nous proposons un nouveau modèle de comportement gustatif des tanins selon leur taille, qui diffère de celui établi par Lea en 1992. Une corrélation entre les teneurs en polyphénols et tanins d'une part et l'astringence des extraits, d'autre part a été trouvée. Dans une moindre mesure, la teneur en tanins est corrélée à l'amertume. Cependant, ces corrélations n'ont pas été observées dans le cas du vin.
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Bikoro, Bi Athomo Arsène. "Analyse et valorisation des coproduits de la transformation industrielle de l’Acajou du Gabon (Khaya ivorensis A. Chev)." Thesis, Pau, 2020. http://www.theses.fr/2020PAUU3001.
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Au Gabon, la forêt recouvre plus de 85% du territoire soit environ 22 millions d’hectares de forêt ce qui représente un potentiel de plus de 400 millions de m3 de bois exploitables. Pour plus de 400 essences répertoriées exploitables, environ 80 sont exploitées, mais seules 13 font l’objet d’une exploitation à l’échelle industrielle. Le bois massif issu de l’exploitation forestière sous forme de grumes était, jusqu’à 2009, principalement destiné à l’exportation. Cependant depuis cette date, l’Etat Gabonais a décidé de développer son industrie, en imposant aux exploitants forestiers d’effectuer au minimum une première transformation dans le pays.Cette, réforme a conduit à l'augmentation de la transformation locale des grumes, et par conséquent à une hausse de la production de co-produits. Ces derniers représentent environ 50% de la masse initiale des grumes en ce qui concerne le sciage et environ 5% pour le déroulage. Les acteurs de la filière doivent actuellement faire face à une nouvelle problématique : un excès de produits connexes générés par la transformation locale du bois.Cette thèse a ainsi pour objectif général de proposer une démarche permettant de trouver des solutions de valorisation de ces co-produits, dont 85% sont actuellement brulés à ciel ouvert. Les co-produits de la transformation industrielle de l’acajou (Khaya ivorensis A. Chev) du Gabon ont été ainsi étudiés en trois phases. Un premier travail a été réalisé sur la caractérisation physico-chimique des extraits d’écorce, d’aubier et de bois de cœur d’acajou. Nous avons particulièrement travaillé sur les extraits phénoliques, dont les tanins. Ensuite, une voie de valorisation de ces composés a été étudiée : l’élaboration d’un adhésif à base de tanins d’acajou. Enfin, une autre piste de valorisation a été explorée : la mise au point d’un composite bois/plastique avec les co-produits de bois et les déchets issus de bouteilles plastiques.Les résultats des différents travaux effectués ont montré que les tanins d’acajou étaient riches en monomères de fisitinidine et de gallocatéchine. L’absence d’acide gallique sous forme libre a aussi été constatée. Par ailleurs, les colles à base de tanins d’acajou ont montré de bonnes caractéristiques thermiques. Enfin, le composite fabriqué a présenté des propriétés physico-mécaniques intéressantes qui permettent d’envisager une valorisation à plus grande échelleThe Gabon forest covers more than 85% of country, so about 22 million hectares of forest, which represents a potential of more than 400 million m3 of exploitable wood. For more than 400 species listed as exploitable, about 80 are exploitable, but only 13 are exploited on an industrial scale. Solid wood from logging in the form of logs was, until 2009, mainly intended for export. However, since that date, the Gabonese State has decided to develop its industry by requiring loggers to carry out at least a first transformation in the country.This reform has led to an increase in local transformation of logs, and therefore an increase in the production of co-products. The latter represent approximately 50% of initial log mass for sawing and around 5% for peeling. The actors of the sectors thus found themselves with the problem of an excess of products derived from the local processing of wood.General objective of this thesis is to propose an approach that makes it possible to find recovery solutions for these by-products, 85% of which are burnt in the open air.Co-products of the industrial transformation of mahogany (Khaya ivorensis A. Chev) from Gabon were thus studied in three phases. Initial work on the physicochemical characterization of bark, sapwood and heartwood of mahogany extracts has been carried out in general. We have particularly work on phenolic extracts, including tannins. Then, a way of valuing these compounds was studied: development of a tannin-based adhesive with mahogany tannins. Finally, another recovery avenue was explored: development of wood/ plastic composite with wood by-products and waste from plastic bottles.Results of various studies have shown that the mahogany tannins are rich in fisitinidin and gallocatechin monomers. The absence of free form of gallic acid was also noted. In addition, tannin-based adhesives of mahogany have shown good thermal characteristics. Furthermore, the composite has interesting physico-mechanical properties that would allow a possible valorization on a large scale
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Miramont, Clément. "Appréciation des tanins, de la couleur et de l'astringence des raisins, moûts et vins par technologies analytiques IRTF et UV-visible couplées à l’analyse de régression multivariée." Thesis, Bordeaux, 2021. http://www.theses.fr/2021BORD0091.
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L'appréciation analytique rapide (quantitative et qualitative) des composés phénoliques des raisins, des moûts et des vins rouges et leur incidence organoleptique (astringence, amertume) apparaît être un challenge en œnologie. Plusieurs méthodes analytiques ont été développées dans cette optique, mais elles nécessitent du matériel, du temps et des connaissances qui ne permettent pas de les appliquer efficacement en routine dans le milieu industriel. Pour optimiser la production des vins rouges en lien avec les concentrations et compositions en anthocyanes et en tanins, nous avons développé des modèles de prédiction fiables grâce à l’IRTF et l’UV-visible couplés à l'analyse de régression multivariée PLS.Afin de répondre à ces attentes, un ensemble d'échantillons représentatifs de la maturité du raisin, de la fermentation alcoolique, de l'élevage en barriques ainsi que des vins commerciaux ont été collectés. Ces échantillons ont été analysés grâce à différentes méthodes décrite dans la littérature, par réaction chimique ou par analyse CLHP-UV-visible, afin d'obtenir la composition et la concentration en flavan-3-ols, la concentration et la composition en anthocyanes, la composition en pigments monomériques et polymériques ainsi que la concentration en tanins totaux, précipitables l’albumine de sérum bovin et la méthylcellulose. Une sélection de vins a également été testée par un panel de dégustateurs entraînés afin de déterminer un indice d’astringence et d'amertume, avec enregistrement des spectres IRTF et UV-visible de ces échantillons.La corrélation entre les analyses spectrales et les différentes informations analytiques obtenues ont été recherchés grâce à l'analyse de régression multivariée PLS, dans le but de concevoir des modèles de prédiction. Les différentes modélisations ont ensuite été testées grâce à une validation croisée, et une validation avec un jeu d'échantillons externes à la calibration.Les résultats obtenus démontrent l'intérêt de l’IRTF et de l’UV-visible couplés à l'analyse de régression PLS pour prédire efficacement les concentrations et compositions en anthocyanes et en tanins des raisins, moûts et vins. Il apparaît que l’UV-visible et plus adapté à la modélisation des concentrations globales, alors que l’IRTF montre un fort potentiel pour une caractérisation plus approfondie de la composition moléculaire. De plus, l’addition à l’IRTF de longueurs d'onde du visible spécifiques a permis d'augmenter la robustesse des modèles pour la prédiction de l'évolution des anthocyanes. Pour la sensation d’astringence, de bons résultats préliminaires ont été obtenus pour l’IRTF et l’UV-visible. L’amertume, en revanche, s’est avéré être une perception trop complexe pour être modélisée.Les limites de prédiction des deux méthodes spectroscopiques ont aussi pu être discutées, notamment la forte variabilité induite par les différents millésimes étudiés qui peut fortement impacter les résultats de prédiction et doit être prise en compte, ainsi que la surinterprétation de résultats prédictifs pour les concentrations en anthocyanes moléculaires qui peuvent amener à questionner certaines modélisations présentes dans la littérature.Nos travaux permettent de mieux appréhender les avantages et les limites de l’IRTF et l’UV-visible couplés à l'analyse de régression PLS pour le dosage des anthocyanes et tanins des raisins, moûts et vins. La possibilité de modéliser grâce à une analyse spectrale une perception telle que l’astringence de façon innovante a aussi été étudiée pour la première fois. L’ensemble des résultats obtenus ont permis d'approfondir les connaissances actuelles sur le sujet et de développer de nouveaux modèles de prédiction fiables, efficaces et transférables pour un usage industriel dans le domaine de la vigne et du vinThe rapid analytical appreciation (quantitative and qualitative) of the phenolic compounds of grapes, musts and red wines and their organoleptic incidence (astringency, bitterness) appears to be a challenge in oenology. Several analytical methods have been developed, but they require equipment, time and knowledge which do not allow them to be applied effectively in routine for industrial environment. To optimize the production of red wines in relation to anthocyanins and tannins concentrations and compositions, we have developed reliable prediction models for FTIR and UV-visible with PLS multivariate regression analysis.In order to obtain these expectations, a set of representative samples from grapes maturity, alcoholic fermentation, aging in barrels and commercial wines were collected. These samples were analyzed using different methods described in the literature, by chemical reaction or by HPLC-UV-visible analysis, in order to obtain the composition and concentration of flavan-3-ols, concentration and composition of anthocyanins, composition of monomeric and polymeric pigments and concentration of total tannins, bovine serum albumin and methylcellulose precipitable tannins. A selection of wines was also tested by a panel of trained tasters to determine an astringency and bitterness index, with FTIR and UV-visible spectra recording of these samples.Correlation between spectral analyzes and the various analytical information obtained were sought with PLS multivariate regression analysis, for designing prediction models. The different models were tested with cross validation, and validation with an external set of samples to the calibration.The results obtained demonstrate the interest of FTIR and UV-visible with PLS multivariate regression analysis to effectively predict anthocyanins and tannins concentrations and compositions in grapes, musts and wines. It appears that UV-visible is more suited to modeling global concentrations, while FTIR shows great potential for the characterization of molecular composition. The addition of specific visible wavelengths to FTIR has increased model robustness for predicting anthocyanin evolution. For astringency sensation, good preliminary results were obtained for FTIR and UV-visible. Bitterness turned out to be a too complex perception to be modelized.Prediction limits of the two spectroscopic methods were also discussed, especially the strong variability induced by different vintages studied who can strongly impact the prediction results and should not be overlooked, as well as the overinterpretation of predictive results for molecular anthocyanin concentrations may lead to questioning some models in the literature.Our work provides better understanding of the FTIR and UV-visible advantages and limits with PLS multivariate regression analysis for determination of anthocyanins and tannins in grapes, musts and wines. The possibility of modeling a perception such as astringency in an innovative way using spectral analysis was also investigated for the first time. All the results obtained have deepen the current knowledge on the subject and they allowed to develop new reliable and efficient prediction models, transferable for industrial use in the vine and wine sector
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Saad, Ahmad. "Les tannins du vins et les lipides de la bouche et du bol alimentaire : vers une modification des marqueurs du goût. Une approche moléculaire et sensorielle." Thesis, Bordeaux, 2017. http://www.theses.fr/2017BORD0879/document.
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Les tannins sont des polymères de polyphénols présents en quantité significative dans le vin rouge, et responsables de l’astringence et de l’amertume. L’astringence est une sensation de sècheresse et de rugosité en bouche résultant d’une forte interaction entre les tannins et les protéines de la salive impliquées dans la lubrification de la cavité buccale. L’amertume, quant à elle, est un goût stricto sensu résultant de l’interaction spécifique des tannins avec les récepteurs du goût situés dans les papilles linguales. Des études récentes ont montré que les tannins sont susceptibles d’interagir avec les lipides. Or les lipides sont présents lors de la dégustation d’un vin comme composants des membranes buccales ou des aliments gras. Cependant, le rôle des lipides dans les perceptions sensorielles d’un vin n’est pas bien connu d’un point de vue œnologique. L’objectif de cette thèse était d’étudier au niveau moléculaire les interactions tannin-lipide, pour mieux comprendre leur rôle dans les propriétés gustatives du vin. Le présent travail décrit l’effet de deux entités représentatives des tannins du vin : un monomère, la catéchine, et un dimère, la procyanidine B1, sur deux modèles lipidiques. Le premier modèle est un modèle membranaire représenté par des vésicules multilamellaires composées de POPC/Cholestérol (70/30), qui mime la composition lipidique des membranes buccales. Le deuxième modèle est une émulsion huile dans l’eau (H/E) stabilisée par le DMPC, qui mime les gouttelettes lipidiques présentes dans les aliments gras. L’organisation et la dynamique des lipides composant ces deux modèles ont été étudiées par la spectroscopie RMN (1H, 2H, 13C) en présence et en absence des deux entités de tannins. Leur localisation dans les membranes lipidiques a également été explorée, de même que leur affinité pour les lipides avec la détermination des constantes d’association tannin-lipide. Les résultats ont mis en évidence un effet fluidifiant des tannins à la fois sur le modèle de membranes buccales et sur le modèle de gouttelettes lipidiques. On a démontré que cet effet de désordre est lié à la nature chimique des tannins, ainsi qu’à leur position dans la membrane. De plus, les résultats sur l’affinité tannin-lipide sont en faveur d’une compétition avec les protéines salivaires. En outre, les résultats de biophysique se sont avérés conformes avec ceux d’une analyse sensorielle menée en parallèle qui a révélé que les aliments gras sont susceptibles de diminuer l’astringence du vin. Ces travaux montrent l’impact des composés phénoliques sur l’ordre membranaire et soulignent pour la première fois un rôle potentiel des lipides sur le goût du vin. D’une part, les interactions tannin-lipide, en perturbant l’environnement lipidique des récepteurs du goût enchâssés dans les membranes buccales, pourraient affecter la fonctionnalité du récepteur et son interaction avec les tannins, et donc l’amertume. D’autre part, une éventuelle compétition entre les interactions tannin-lipide et tannin-protéine de la salive pourrait diminuer l’astringence durant la dégustation d’un vin. Dans le domaine de l’œnologie, cette thèse vient étayer le ressenti des dégustateurs à savoir la modification du goût du vin due aux aliments et ouvre de nouvelles perspectives dans le cadre de l’association mets-vinsTannins are polyphenol polymers present in significant amounts in red wine responsible for astringency and bitterness. The former is a tactile perception involving dryness and roughness in the mouth due to the interaction between tannins and saliva proteins and the latter is a primary taste due to the interaction between tannins and taste receptors in taste buds. Tannins are now known to also interact with lipids. Although not present in wine, lipids are yet present during tasting in the oral membranes of tasters and in fatty foods when wine is consumed during a meal. However, although the influence of lipids is well known to wine tasters through food pairing, there is no scientific evidence to support this hedonic feeling. The aim of the thesis is to study tannin-lipid interactions at molecular level in order to better understand their implication in wine gustative properties. The present work describes the effect of the main representative grape tannin subunits, the catechin monomer and the B1 dimer, both on a model of oral membranes and food fat globules. They are represented by a dispersion of POPC/cholesterol multilamellar vesicles and a olive oil in water emulsion stabilized by DMPC as emulsifier, respectively. The organization and dynamics of the lipids composing these two models were investigated by solid-state NMR spectroscopy (1H, 2H, and 13C) in the absence and the presence of the two tannin subunits. The affinity of tannins for lipids was also explored by the determination of the thermodynamic association constant. The results pointed out a fluidizing effect of tannins both on the membrane model, as previously shown on a simpler membrane model, and on the emulsion lipid droplets. The disorder caused by tannins was shown to be related to their location in the lipid structure depending on the tannin chemical nature. Moreover, the strength of the interaction between tannins and membrane lipids was revealed to be in the same order of magnitude of that between tannins and saliva proteins. In addition, the biophysical results were in accordance with those of a sensory analysis led in parallel that revealed that fatty foods are prone to decrease wine astringency. These pioneering works shows the impact of phenolic compound on membrane order and highlight for the first time the potential role of the tannin-lipid interactions on wine taste. On the one hand, by disrupting the lipid environment of taste receptors embedded in oral membranes, tannin-lipid interactions could affect the receptor functionnality and therefore the interaction with tannin molecules, so bitterness. On the other hand, the existence of a possible competition between lipids and saliva proteins for interacting with tannins during tasting could reduce astringency
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Lacampagne, Soizic. "Localisation et caractérisation des tannins dans la pellicule du raisin : impact de l'organisation physico-chimique des parois cellulaires sur la composante tannique, la qualité du fruit et la typicité des raisins de Bordeaux." Thesis, Bordeaux 2, 2010. http://www.theses.fr/2010BOR21762/document.
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Qualité du fruit et la typicité des raisins de Bordeaux.La maturation de la pellicule du raisin est un phénomène complexe, caractérisé par de nombreux changements structuraux importants et par l’accumulation de composés œnologiques tels que les tannins. Malgré leurs propriétés biologiques et organoleptiques essentielles pour la qualité des fruits et des vins, peu de travaux concernent l’organisation et la localisation de ces composés au sein du tissu pelliculaire. Dans la pellicule, les tannins peuvent être plus ou moins liés aux constituants cellulaires modulant ainsi leur extractibilité lors des processus de vinification. Notre travail, basé sur des approches biochimiques, moléculaires et microscopiques, apporte des données nouvelles sur la biosynthèse, la localisation et l’organisation des tannins dans la pellicule. Au sein de ce tissu, nous avons souligné l’importance des tannins pariétaux et montré que leurs caractéristiques physico-chimiques dépendaient entre autre des changements structuraux des parois. Ainsi, cette organisation caractérise en partie la texture de la pellicule et conditionne la qualité du fruit. Pour appréhender l’évolution des critères de texture de la pellicule au cours de la maturation du raisin, l’analyse sensorielle des baies et la mesure par pénétrométrie s’avèrent être des outils pertinentsSkin grape maturation is a complex phenomenon, characterized by an important number of structural changes as well as by phenolic compounds accumulation (i.e. tannins). Despite their biological and organoleptic properties in fruit and wine quality, few studies report both tannins organization and localization in skin tissue. In skins, tannins may be more or less related with cellular components, modulating their extractability during winemaking process.Our work, based on biochemical, molecular and microscopic approaches reveals new data on skin tannins biosynthesis, localization and organization. Within this tissue, we highlighted the parietal tannins importance, we evidenced that their physicochemical characteristics depend among other structural changes on cell walls. Thus, this organization partially characterized the skin texture and affects fruit quality. To understand the evolution criteria for skin texture during grape berry ripening, sensory analysis and measurements by penetrometer proved to be relevant tools
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Peronny, Sylvie. "La perception gustative et la consommation des tannins chez le maki (Lemur catta)." Phd thesis, Museum national d'histoire naturelle - MNHN PARIS, 2005. http://tel.archives-ouvertes.fr/tel-00125461.
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Le comportement alimentaire des primates varie en fonction de la disponibilité des ressources donc des saisons, et du choix de l'animal, facteur lié à des paramètres individuels. Les aliments consommés sont choisis en fonction de leur chimie (présence de nutriments, de composés toxiques...), et c'est le système gustatif qui permet de faire le lien entre un primate et les aliments qu'il consomme. Les tannins, toxiques à forte dose, sont les composés secondaires des plantes les plus répandus. Le Lemur catta, primate emblématique de Madagascar y est confronté dans son milieu naturel. L'étude de lémurs catta captifs, confrontés à des plantes de climat tempéré riches en tannins (chênes, saules, ronces) montre qu'ils consomment spontanément des tannins dont le goût est perçu comme répulsif (dès 0,1 g/L pour l'acide tannique, seuil déterminé par le « two-bottle test »). Les quantités de tannins consommés et la concentration en tannins du régime varient en fonction des saisons alors que la perception du goût de l'acide tannique ne varie pas. Par contre, les différences individuelles de consommation de tannins sont expliquées par les différences de perception gustative : les animaux les plus sensibles aux tannins en sont les plus gros consommateurs.
La consommation de terre observée quasi-quotidiennement (principalement le matin, souvent à jeun) est corrélée avec celle de plantes à tannins, la géophagie serait alors un moyen de protection utilisé par les lémurs catta, les argiles ayant la capacité d'adsorber les tannins. Les tannins condensés consommés pourraient jouer un rôle dans la diminution avec le temps du parasitisme des individus. La semi-liberté associée à un accès à des plantes variées apporte de nombreux avantages aux lémuriens captifs car elle leur permet de diversifier leur alimentation et d'avoir accès à certaines activités pratiquées par les lémuriens sauvages.
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Le, Floch Alexandra. "Les polysaccharides et les ellagitanins du bois de chêne : influence sur la qualité sensorielle des vins." Thesis, Bordeaux, 2015. http://www.theses.fr/2015BORD0369/document.
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Il est généralement admis que la qualité des vins et des eaux de vie dépend de leur vieillissement au contact du bois de chêne. La sélection du bois de chêne et sa maturation constituent des étapes essentielles dans la fabrication de barriques. Compte tenu des nombreux facteurs impliqués dans le choix de la matière première et dans la phase de séchage naturel du bois de chêne, il est très complexe de déterminer l’impact de la durée de séchage et l’incidence des facteurs de sélection combinés. Nous avons pu mettre en œuvre un plan d’essai qui nous a permis d’étudier l’évolution de la composition chimique du bois de chêne pour quatre stades de séchage : bois frais, 12 mois, 18 mois et 24 mois. Pour ce plan d’essai, l’échantillonnage a été réalisé en prenant en compte trois facteurs de sélection d’intérêt en tonnellerie : l’âge du bois, le type de grain et la classe d’Indice Polyphénolique mesurée par Oakscan®. En plus de la partie extractible (~10%), trois polymères constituent la majeure partie (~90%) du bois de chêne : la cellulose, les hémicelluloses et les lignines. Ces composés sont susceptibles de subir des hydrolyses ou des réactions chimiques au cours des différents processus de tonnellerie et notamment lors de la chauffe, libérant ainsi des composés aromatiques ou certains précurseurs aromatiques ayant un véritable intérêt sensoriel pour le vin vieilli en fût ou en contact avec du bois de chêne. Aucune étude n’a, à ce jour, montré un lien entre les proportions de ces composés dans le bois de chêne et l’impact au niveau chimique et organoleptique dans des vins élevés au contact de bois de chêne. Nous avons pu montrer que leurs proportions évoluent de façon significative au cours du séchage du bois de chêne et nos résultats soulignent l’incidence des différents facteurs de sélection. Respectivement, les proportions en composés extractibles, lignines, hémicelluloses et cellulose apparaissent majoritaires aux stades bois frais, 12 mois, 18 mois et 24 mois de séchage. La mise en place d’un plan d’essai sur un vin de Merlot à partir d’un deuxième échantillonnage de bois de chêne, selon les mêmes modalités que le plan d’essai sur bois, nous a permis d’évaluer l’impact du temps de séchage du bois sur la composition chimique d’un vin élevé au contact de morceaux de bois de chêne. Les résultats ont montré entre autres une baisse de près de 8% de teneur en ellagitanins moléculaires du vin entre les modalités 12 et 24 mois de séchage mais aussi des impacts sur les concentrations en composés volatiles du vin : les aldéhydes furaniques sont corrélés positivement à des temps de séchage longs (18 et 24 mois), tandis que les aldéhydes phénoliques le sont avec un temps de maturation plus court (12 mois). Nos résultats soulignent aussi l’influence des facteurs de sélection sur la composition chimique du vin. Sur le même vin de Merlot, la réalisation d’analyses sensorielles a mis en évidence des différences significatives perçues par notre panel entre les modalités de séchage 12 et 24 mois, tandis que les modalités 18 et 24 mois n’ont pas été distinguées lors de tests triangulaires. Des profils sensoriels ont également été établis pour tenter d’associer les différences perçues à un ou plusieurs descripteurs. Pour cette épreuve, les résultats soulignent la difficulté pour notre panel de dégustateurs de s’accorder sur l’existence d’une différence globale et significative entre les modalités 12 et 24 mois. Cependant, ils contribuent à expliquer les différences mises en évidence lors des tests triangulaires pour certaines modalités de séchage pour les descripteurs grillé/fumé, amertume, astringence, rondeur en bouche et perception sucréeIt is widely accepted that alcoholic beverages quality depends on their ageing in premium quality oak wood. Oak wood selection and maturation are essential steps in the course of barrel fabrication. Given the existence of many factors involved in the choice of raw material and in natural seasoning of oak wood, it is very difficult to determine the real impact of seasoning and selection factors on oak wood composition. A sampling was done to study the evolution of oak wood chemical composition during four seasoning steps: non matured, 12 months, 18 months and 24 months. For this sampling, three selection factors were taken into account: age, grain type and the Polyphenolic Index measured by Oakscan®. Besides extractables (~10%), three polymers constitute the main part of oak wood: cellulose, hemicelluloses and lignins. These compounds may undergo hydrolysis or chemical reactions during cooperage processes, especially during heat treatment, which release some aromatic compounds or aromatic precursors having a genuine sensorial interest on wine aged in barrel or in contact with oak products. To date, no studies revealed a link between the proportions of these compounds in oak wood and the chemical and sensorial impact in wines ageing with oak wood. Our study showed that the proportions of these compounds evolved significantly during oak wood seasoning and the results highlighted the impact of selection factors. Respectively, extractables, lignins, hemicelluloses and cellulose proportions were mostly for non-matured, 12 months, 18 months and 24 seasoning months. The development of a test plan with a Merlot wine from a second oak wood sampling, using similar modalities as the previous test plan allowed the evaluation of oak wood seasoning impact on the chemical composition of a wine ageing with oak wood pieces. Results showed a lowering of 8% in ellagitannins content of wine between 12 and 24 months modalities. An impact on volatile composition in wine has also been established: furanic aldehydes were positively correlated with a long maturation time (18 and 24 months), whereas phenolic aldehydes were positively correlated with a shorter seasoning time (12 months). Our results highlighted also the impact of selection factors on wine chemical composition. Sensorial analysis on this Merlot wine led to significant differences detected by the panel between 12 and 24 seasoning modalities, but not between 18 and 24 months modalities with triangular tests. Sensorial profiles were also established to attempt to associate the differences detected to one or more descriptors. For this test, results highlighted the difficulty for the panel to make a significant difference between 12 and 24 months modalities. However they contribute to explain the differences demonstrated with triangular tests for some seasoning modalities: toasted, bitterness, astringency, roundness and sweet perception
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Mole, Simon. "Tannins : a biochemical re-analysis of their importance as anti-feedants." Thesis, University of Strathclyde, 1986. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=21472.
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Tannins have long been thought of as antifeedants owing to their presumed digestibility-reducing properties. In this thesis information, at the molecular level, is presented in a reassessment of this assumption and the apparency theory of plant chemical defence which is dependent upon it. An introductory review provides chemical and opperational descriptions of tannins and a general outline of their ecological impact. Detailed attention is given to (i) tannin-protein complex formation and (ii), an assesment of in vivo evidence concerning the effects of tannins on herbivores. It is concluded that the evidence does not support the hypothesis that tannins uniformly reduce digestion, even though they do generally act as antifeedants. A series of crude tannin-containing plant extracts were prepared and characterised by chemical analyses and by their ability to precipitate protein and inhibit pepsin/cellulase digestion of celulose. Results indicated between-tannin variation but not that the chemical properties of crude tannins might be used to predict their interaction with the other components of a herbivore's diet. Experiments under conditions where soluble tannin-protein complexes formed and which modelled some digestive systems, showed that tannins could under varying circumstances, inhibit or promote the digestion of protein. Soluble tannin-protein complexes were also formed in the presence of bile salts when they would otherwise have occured as precipitates. In these conditions clear relief from digestibility reduction was found. In the light of these results a new model describing the effects of tannins on digestion, consistent with results obtained in vivo, is proposed.
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SILVA, Crissanny Inês de Oliveira. "Relação temporal de componentes morfológicos e composição química em plantas da caatinga, Serra Talhada – PE." Universidade Federal Rural de Pernambuco, 2012. http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6723.
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Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq
This study aimed to characterize bromatological and morphological components (leaves and stems) of some native plants during different periods of the year. Samples were collected at the farm located in San Miguel Serra Talhada PE. In Caatinga area grazed by sheep throughout the year. The samples were collected at intervals of approximately 45 days, from January to August 2011, totaling five evaluation. The evaluated the following species were: Catingueira (Caesalpinia pyramidalis Tui.), Jurema preta (Mimosa hostilis Benth.), Mororó (Bauhinia cheilantha), Marmeleiro (Cydonia oblonga), Malva branca (Sida cordifolia), Orelha de onça (Macroptilium martii), Capa bode (Ipomeae carnea Jacq.) e Pereiro (Aspidosperma pyrifolium). Sampling was conducted simulating grazing animals, collecting leaves and branches separately, three random samples from each species were collected, consisting of plant material with approximately 2 mm thick and picked up to 1.5 m tall. Average CP observed for the evaluated species were above the critical limits described by the literature for leaf fraction (10.5%) however, CP for the branch fraction was 4.4%. Ashes presented higher overall average of species for leaf fraction (7.7%) compared with the branch fraction (5.6%). There was an increase in the fraction of NDF throughout the research, which showed overall average among species ranged from 46.2 and to 67.1% from January and to August 2011, respectively. In variable tannin there was also an increase during the search among the plants studied. During the year, the chemical composition and tannin content of these forages undergo variations, which can interfere with the quality of these plants and consequently in animal feed.
Objetivou-se caracterizar bromatologicamente componentes morfológicos (folhas e ramos) de algumas plantas nativas durante diferentes períodos do ano. As coletas foram realizadas na fazenda São Miguel situada em Serra Talhada - PE, em área de Caatinga pastejada por ovinos durante todo ano. As coletas das amostras foram realizadas em intervalos de aproximadamente 45 dias no período de janeiro a agosto de 2011, totalizando cinco períodos de avaliações. Foram avaliadas as seguintes espécies: Catingueira (Caesalpinia pyramidalis Tui.), Jurema preta (Mimosa hostilis Benth.), Mororó (Bauhinia cheilantha), Marmeleiro (Cydonia oblonga), Malva branca (Sida cordifolia), Orelha de onça (Macroptilium martii), Capa bode (Ipomeae carnea Jacq.) e Pereiro (Aspidosperma pyrifolium). A amostragem foi realizada simulando pastejo dos animais, coletando as folhas e ramos separadamente, sendo colhidas três amostras aleatórias de cada espécie vegetal, constituídas de material com aproximadamente 2 mm de espessura e colhidos até 1,5 m de altura. Para a variável PB a média geral obtida entre as espécies, foi superior aos limites críticos descritos pela literatura consultada para fração folha 10,5% diferentemente da média geral entre espécies na fração ramos, que foi de 4,4%. A MM apresentou média geral das espécies maior para fração folha 7,7% quando comparado com fração ramos 5,6%. Observou-se aumento de FDN na fração folha ao longo da pesquisa onde apresentou média geral entre espécies de 46,2 e 67,1 % no mês de janeiro e mês de agosto de 2011. Na variável tanino também houve um aumento durante a pesquisa entre as plantas estudadas. Durante o ano, a composição química e teores de taninos destas forrageiras sofrem variações, que podem interferir na qualidade destas plantas e consequentemente no consumo animal.
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Manolaraki, Foteini. "Propriétés anthelminthiques du sainfoin (Onobrychis viciifoliae) : analyse des facteurs de variations et du rôle des composés phénoliques impliqués." Thesis, Toulouse, INPT, 2011. http://www.theses.fr/2011INPT0011/document.
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Les nématodes parasites du tube digestif demeurent une contrainte majeure pesant sur la santé, le bien être et les productions des petits ruminants élevés à l’herbe. Le mode usuel de maîtrise de ce parasitisme repose sur l’emploi répété de molécules chimiques anthelminthiques. Toutefois, le développement et la diffusion généralisée de résistances à ces molécules dans les populations de vers imposent désormais d’utiliser ces traitements avec discernement et de trouver des solutions complémentaires ou alternatives. L’incorporation dans la conduite d’élevage (ration des moutons ou des chèvres) de légumineuses fourragères riches en tannins condensés dotées de propriétés anthelminthiques s’est avérée une option prometteuse pour réduire le recours aux molécules chimiques. Toutefois, une des difficultés d’application de ces plantes tient à la variabilité des résultats observés. En prenant le sainfoin comme modèle de légumineuse contenant des tannins et en s’appuyant essentiellement sur des méthodes in vitro basées sur les larves 3 infestantes, l’objectif général de cette thèse est d’analyser le rôle respectif de facteurs liés à l’environnement, aux variétés génétiques ou aux modes de préservation technologiques sur les propriétés anthelminthiques. Quelque soit le critère envisagé, une forte variabilité a été observée. Les principales variations liées à l’environnement dépendaient de l’année et du cycle de coupe, ainsi que du site d’exploitation. Parmi les 38 variétés testées, 9 se sont avérées à forte activité AH, alors que 22 étaient à très faible activité. Enfin, les résultats ont aussi surtout souligné une plus forte activité antiparasitaire dans des formes séchées ou ensilées par comparaison à des échantillons frais. La comparaison des profils biochimiques liés à cette variabilité a permis une exploration des composés phénoliques expliquant l’activité anthelminthique. L’existence d’une relation dose-réponse a été précisée. Le rôle des tannins condensés a été confirmé, notamment ceux à faible degré de polymérisation et à faible poids moléculaire. L’importance des prodelphinidines qui seraient plus actives que les procyanidines reste à confirmer. De plus, il a été montré que d’autres flavonoides peuvent jouer un rôle, notamment les flavan-3-ols et les flavonols. La différence d’activité entre les échantillons de sainfoin ensilés ou fanés par comparaison aux échantillons frais s‟expliquerait en partie par la présence de formes non glycosidés de flavonols. Ces résultats devraient conduire à développer des méthodes de dosage pour identifier les échantillons de sainfoin, et plus généralement de légumineuses riches en tannins, dotés de propriétés anthelminthiques significativesGastrointestinal nematodes remain a major constraint on the health, welfare and production of small ruminants. Over the past decades, the usual mode of control of this parasitism has mainly relied on the repeated use of chemical anthelmintics. However these treatments are nowadaysfacing some limits among which the most important is the development and widespread diffusion of resistance to these chemical molecules within worm populations. Consequently, the need to find complementary or alternative solutions is becoming urgent. The possible exploitation of forage legumes, rich in condensed tannins, with anthelmintic properties, by incorporation in the diet of sheep or goats, seems a promising option to reduce the reliance on chemical molecules. However, one of the main difficulties to use these plants as nutraceuticals relates to the variations in results. By using sainfoin (Onobrychis viciifoliae) as a model of tannin-containing legume and based on in vitro methods on the infective third stage larvae, the main objectives of this PhD were i) to examine the influence of environmental, genetic (40 different varieties) and technological (mode of preservation) factors on the anthelmintic properties of sainfoin and ii) to analyse whether differences in phenolic compounds might explain the variations. Whatever the factor considered, a high variability in results was observed. The main variations due to the environmental factors depended on the year and the cycle of cutting, as well as on the site of cultivation. Among the 38 varieties tested, only 9 have shown AH activity over 50 %. Last, a higher antiparasitic activity was found in the dried or ensiled forms compared to the fresh samples. A comparison of the biochemical profiles associated with these variations indicated a role of proanthocyanidins plus other phenolic compounds in the anthelmintic properties. The dose-response relationship between the AH activity and the ability to form complex with proteins was defined. The role of condensed tannins was confirmed, particularly those with a low degree of polymerization. The respective importance of prodelphinidins vs procyanidins remains to be further investigated. Moreover, the possible role of other flavonoids, in particular of flavan-3-ols and flavonols was also confirmed. The difference in activity between dried or ensiled forms compared to fresh sainfoin samples was partly explained by the presence of flavonol aglycosides. These results should favour the development of measurements in order to identify sainfoin samples with higher anthelmintic properties
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Pelletreau, Karen Nicole. "The application of molecular tools towards the study of brown algal chemical ecology and the production of phlorotannins." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 230 p, 2008. http://proquest.umi.com/pqdweb?did=1601234901&sid=4&Fmt=2&clientId=8331&RQT=309&VName=PQD.
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Åkesson, Karin. "Analys av tanniner : från granbarksextrakt." Thesis, Linköping University, Department of Physics, Chemistry and Biology, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-51726.
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The objective for this master’s thesis was to test and evaluate two methods for determining the content of tannin in a pinebarkextract. The methods used at Södra for this previously have not been specific enough, only the amount of polyphenolics have been measured. One of these methods is a test based on the Stiasny test and it determines the extracts ability to form a gel with formaldehyde. When this test was carried out it showed that the extract did not contain much tannin. The other method used at Södra measures the amount of polyphenolic substances with a spectrophotometer. The result from this method showed that the content were 50 %.
One of the two new methods that were evaluated determines the amount tannin present in the extract because of tannins ability to form a complex with proteins. According to this method, the amount tannin in the extract were 42,5 %, and 19,4 % of this in the form of tannic acid. These results are credible on basis of previous information about the extract.
The other method uses a RP- HPLC where ellagic and gallic acid were used as standards. The amount ellagic acid in the extract was determined to 0,06 %, but the result from gallic acid was inconclusive. Further analysis is necessary to evaluate the obtained results and the methods reliability.
If the metods is to be used on a regular basis, my recommendation would be to start evaluating the protein-binding method because this would be easier and less time-consuming than modifying the HPLC- method. The HPLC- method could on the other hand provide useful information about the extract, not only the amount tannin could be measured, but also which kind of tannin could be investigated.
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Godoy, Patrícia Barboza de. "\"Aspectos nutricionais de compostos fenólicos em ovinos alimentados com leguminosas forrageiras\"." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/64/64132/tde-16052007-135551/.
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As leguminosas constituem uma importante fonte de alimentos para os ruminantes e podem ser exploradas para pastejo direto ou, se conservadas, para fornecimento na forma de feno ou silagem. Algumas leguminosas possuem compostos fenólicos em sua composição. A caracterização química dessas as plantas possibilita melhor uso das mesmas na alimentação animal assim como um melhor entendimento dos efeitos positivos e negativos dos compostos fenólicos na nutrição dos animais. Objetivou-se com o presente estudo: (i) avaliar a composição química de cinco leguminosas de interesse para a alimentação de ruminantes; (ii) estudar os efeitos dos taninos de diferentes leguminosas na produção de gases in vitro; e (iii) estudar os efeitos de dietas constituídas com estas leguminosas no consumo voluntário e digestibilidade aparente dos nutrientes em ovinos. O primeiro trabalho (Capítulo 3) refere-se à caracterização in vitro das leguminosas forrageiras (Leucaena leucocephala (Leucena), Arachis pintoi (Amendoim forrageiro), Stylosanthes guianenses cv mineirão (Estilosantes mineirão), Stylosanthes guianenses cv Campo Grande (Estilosantes Campo Grande e Calopogonio sp. (Calopogônio). Foram avaliadas a composição química, a quantificação de taninos, a fermentabilidade ruminal e a síntese microbiana e os resultados obtidos demonstraram valores de proteína bruta compatíveis com a literatura, exceto para o Calopogônio e Estilosantes Campo Grande (< 60 g kg-1 MS). Os teores de taninos variaram significativamente dentre as plantas estudadas, entretanto o teor de tanino condensado pode ser considerado seguro para os animais (entre 30 a 40 eq-g leucocianidina kg-1 MS). As cinco leguminosas apresentaram boa fermentabilidade in vitro, com baixo tempo de colonização (~ 4 h) e T ½ (tempo gasto para atingir metade do valor da Produção Potencial de Gases) inferior a 25 h. A técnica in vitro de incorporação de radiofósforo mostrou efeito significativo da adição de polietileno glicol (PEG) na avaliação da síntese microbiana, demonstrando o efeito dos taninos presentes nas leguminosas estudadas sobra a síntese de proteína no rúmen. O segundo trabalho (Capítulo 4) refere-se à caracterização e avaliação nutricional in vitro de Medicago sativa (Alfafa), Cajanus cajan (Feijão guandu), Mucuna aterrina (Mucuna preta) e Mucuna pluriens (Mucuna cinza). A composição química, a quantificação de taninos e a cinética de fermentação destas leguminosas e dietas experimentais constituídas de fenos de Tifton-85 (Cynodon sp), milho triturado, sal mineralizado na proporção de 30:18:2 com a adição (50%) de cada uma das leguminosas foram estudadas (tratamento ALF, GND, MCZ, MPT respectivamente para Alfafa, Feijão guandu, Mucuna cinza e Mucuna preta). Alfafa apresentou o maior conteúdo de proteína bruta (185 g kg-1 MS) e os teores de taninos variaram significativamente entre as plantas estudadas. Mucuna cinza apresentou maior e Alfafa menor valores de tanino condensado (50 e 0,2 eg. G leucocianidina kg-1 MS respectivamente). Com exceção da Alfafa, todas as outras leguminosas apresentaram incremento de gases quando incubadas in vitro na presença de PEG; o que reflete a atividade biológica dos taninos presente nestas plantas. Em relação às dietas experimentais, apenas as dietas GND e MCZ apresentaram incrementos de gases na presença de PEG. O terceiro trabalho (Capítulo 5) descreve a avaliação nutricional in vivo das dietas experimentais utilizadas no Capítulo 4 (ALF, GND, MCZ, e MPT). Foram utilizados ovinos da raça Santa Inês, machos, castrados com peso vivo médio de 53 ± 5,1 kg. As dietas foram oferecidas de acordo com o peso vivo de cada animal (3%) durante o ensaio de consumo voluntário, enquanto que durante o ensaio de digestibilidade, foi oferecida em cerca de 90% do consumo voluntário determinado anteriormente. Nenhuma dieta apresentou quantidade de taninos condensados considerado prejudicial aos animais. Todas as dietas estudadas apresentaram consumo voluntário e consumo de nutrientes semelhantes. Apenas ALF apresentou maior digestibilidade de PB, enquanto que a MCZ apresentou menor digestibilidade da mesma fração. É concluído com esses trabalhos que as leguminosas estudadas constituem uma importante fonte de nutrientes para os ruminantes, contudo, os teores de taninos condensados devem ser monitorados a fim de que sejam preparadas dietas que não alterem o consumo voluntário nem a fermentação ruminal, e consequentemente disponibilização dos nutrientes aos animaisLeguminous plants constitute an important source of food for the ruminants and they can be explored as direct grazing or, if conserved, for supply in the form of hay or silage. Some of those plants possess phenolic compounds in its composition. The chemical characterization of such plants make possible better use of them in the animal feeding as well as a better understanding of the positive and negative effects of the phenolic compounds in the nutrition of the animals. It was aimed with the present study: (i) to evaluate the chemical composition of five leguminous plants of interest for the feeding of ruminant; (ii) to study the effects of the tannins of different leguminous plants in the in vitro gas production assay; and (iii) to study the effects of diets constituted with these leguminous plants in the voluntary intake and apparent digestibility of the nutrients in sheep. The first work (Chapter 3) refers to the characterization in vitro of the leguminous foragers (Leucaena leucocephala (Leucena), Arachis pintoi (Amendoim forrageiro), Stylosanthes guianenses cv mineirão (Estilosantes mineirão), Stylosanthes guianenses cv Campo Grande (Estilosantes Campo Grande) and Calopogonio sp. (Calopogônio). They were appraised for the chemical composition, the quantification of tannins, the ruminal fermentability and the microbial synthesis. The obtained results demonstrated compatible values for crude protein content, except for Calopogônio and Estilosantes Campo Grande (< 60 g kg-1 DM). The tannin content varied significantly among the studied plants, however the condensed tannin content can be considered safe for the animals (between 30 to 40 eq-g leucocianidin kg-1 DM). The five leguminous plants presented good in vitro fermentability, with low time of colonization (~ 4 h) and T ½ (time spent to reach half of the value of the potential gas production) less than 25 h. The in vitro technique of radio labeled phosphorus incorporation showed significant effect of the addition of polyethylene glycol (PEG) in the evaluation of the microbial synthesis, demonstrating the effect of the presence of tannins in the studied leguminous plants. The second work (Chapter 4) refers to the characterization and in vitro nutritional evaluation of Medicago sativa (Alfalfa), Cajanus cajan (Feijão guandu), Mucuna aterrina (Mucuna preta) and Mucuna pluriens (Mucuna cinza). The chemical composition, the quantification of tannins and the kinetics of fermentation of these leguminous plants and four constituted experimental diets with Tifton-85 hay (Cynodon sp), corn grain, mineralized salt (30:18:2) with the addition (50%) of each one of the leguminous plants were studied (treatment ALF, GND, MCZ, MPT respectively for Alfalfa, Feijão guandu, Mucuna cinza and Mucuna preta). Alfalfa presented the highest content of crude protein (185 g kg-1 DM) and the tannin content varied significantly among the studied plants. Mucuna cinza presented greater and Alfalfa lower values of condensed tannin (50 and 0.2 eg g leucocianidin kg-1 DM respectively). Except for the Alfalfa, all the other leguminous plants presented increment of gases when incubated in vitro in the presence of PEG; what reflects the biological activity of the tannins present in these plants. In relation to the experimental diets, just the diets GND and MCZ presented increments of gases in the presence of PEG. The third work (Chapter 5) describes the in vivo nutritional evaluation of the experimental diets used in the Chapter 4 (ALF, GND, MCZ, and MPT). Santa Inês males, castrated with live weight of 53 ± 5.1 kg sheep were used. The diets were offered in agreement with the liveweight of each animal (3%) during the voluntary intake assay, while during the digestibility trial, it was offered in about 90% of the voluntary intake determined previously. No diet presented amount of condensed tannins considered harmful to the animals. All of the studied diets presented similar voluntary intake and nutrients consumption. Just ALF presented higher crude protein digestibility, while MCZ presented lower digestibility of the same fraction. It is concluded with those works that the studied leguminous plants constitutes an important source of nutrients for the ruminants, however, the condensed tannins content should be monitored so that it can prepare diets to neither alter the voluntary intake nor the ruminal fermentation, and consequently guaranteeing the supply of nutrients to the animals
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Nel, Anton Pieter. "The influence of different winemaking techniques on the extraction of grape tannins." Thesis, Stellenbosch : University of Stellenbosch, 2011. http://hdl.handle.net/10019.1/6872.
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Thesis (MScAgric (Viticulture and Oenology))--University of Stellenbosch, 2011.Includes bibliography.
ENGLISH ABSTRACT: Grape and wine phenols consist of flavanols which is the building blocks for tannins. These building blocks are called monomers which consist of catechins, epicatechins, epigallocatechins and epicatechin-gallate. Tannin is important in wine as it contributes to bitterness, mouth feel (astringency) and maturation potential of the wine. Futhermore it has a health benefit as an antioxidant. Anthocyanins are responsible for the colour of red wine. The anthocyanins combine with tannins to form stable polymeric pigments. Due to the importance of tannins and anthocyanins in wine, it is imperitative that different winemaking techniques are used to extract as much of these components as possible and that the analysis is done quickly and accurately. The aim of this study was to evaluate different winemaking techniques and their extraction of tannins and anthocyanins into the wine. Too much tannin extraction can have a negative effect on the sensory quality of the wine. Therefore a second aim was to evaluate the mouth feel properties of a Shiraz wine. A third aim was to compare the two tannin precipitation methods in terms of time efficiency, repeatability and the ease of practice. To investigate the amount of tannin concentration extracted by different winemaking techniques, two cultivars (Cabernet Sauvignon and Shiraz) were used. These treatments included the addition of an enzyme during fermentation [E], cold maceration [CM], post maceration [PM] and the combination of cold and post maceration [CM+PM]. The grapes were harvested in two different climatic areas during the 2008 and 2009 vintages. The two climatic areas were classified according to the Winkler scale as a III (Morgenster) and a IV (Plaisir de Merle). The grapes were harvested at two different ripeness levels in order to evaluate the effect of the different winemaking processes on the extraction of tannins and anthocyanins. One harvest was before (LB) and the other after (HB) the commercial harvest. The results of this study showed significant differences in the phenolic composition of the wines. It was found that the warmer area showed higher tannin concentrations than the cooler area for both cultivars. In the 2008 Cabernet Sauvignon the CM extracted higher concentrations of tannin from the cooler area at both ripeness levels. In the warmer area, CM extracted the highest tannin concentration HB, but the CM+PM extracted the highest tannin concentration from Cabernet Sauvignon at the LB and CM at the HB of the warmer area. In 2009 the PM extracted the highest concentration of tannin at the lower ripeness level, while the E treatment extracted the highest concentration from the warmer area. In the cooler area the CM+PM extracted the highest concentration of tannin at a lower ripeness level, while there were no siginicant differences between the different treatments at the higher ripeness level. The highest anthocyanin concentration was found in the cooler area. The CM treatment was found to have no effect on anthocyanin extraction. Different methods are available to quantify the tannin concentration in wine. Two of the most popular tannin analytical methods are the bovine serum albumin (BSA) and the methyl cellulose precipitable tannin (MCP) methods. The BSA method is a very complex method which uses at least 3 times more reagents than the MCP method. The MCP method only analyzes tannins, while the BSA method analyzes tannins, monomeric pigments (MP), small polymeric pigments (SPP) and large polymeric pigments (LPP). In this study a good correlation was found between the two tannin precipitation methods (R2 – 0.88). There is controversy regarding the variability of these methods. Some scientists found that the two methods show a good correlation with HPLC, while others found that there was no such correlation between the precipitation methods and the HPLC. The MCP method had a practical advantage as it could be performed in half the time required for the BSA method. This has a significant impact in scenarios where a high sample throughput is required although it only measures total tannin. The phenolic composition and mouth feel of the wine was strongly influenced by the climatic area. In the warmer area the effect of tannin concentration on mouth feel was much less than in the cooler area. The wine made of riper grapes, was more grippy, bitter and numbing than the wines made from greener grapes. The E treatment was especially associated with a dry, grippy sensation.
AFRIKAANSE OPSOMMING: Druif en wyn fenole bestaan uit flavanole wat weer die boublokke is van tanniene. Hierdie boublokke, wat bekend staan as monomere, betsaan uit katesjiene, epikatesjiene, epigallokatesjiene an epikatesjien-gallaat. Tanniene is belangrik in wyn aangesien dit bydra tot bitterheid, mondgevoel (vrankheid) asook die verouderingspotensiaal van wyn. As antioksidante hou dit ook gesondheidsvoordele in. Antosianiene dra by tot die kleur van rooiwyn. Antosianiene kombineer met tanniene om meer stabiele polimeriese pigmente te vorm. As gevolg van die belangrikheid van tanniene en antosianiene is dit van uiterse belang dat verskillende wynmaak tegnieke gebruik word om ekstraksie in die wyn te bevoordeel en dat die analitiese metode so vinnig en akkuraat as moontlik gedoen word. Die eerste doel van hierdie studie was om die ekstraksie van tanniene en antosianiene deur middel van verskillende wynmaak tegnieke te evalueer. Te veel tanniene in die wyn kan negatiewe sensoriese kwaliteit tot gevolg het. Daarom is die tweede doel om die sensoriese kwaliteit van Shiraz wyn te evalueer. Die derde doel van hierdie studie was die twee tannien presipitasie metodes met mekaar te vergelyk in terme van die moeilikheidsgraad van die metode, tyd doeltreffendheid en herhaalbaarheid. Verskillende wynmaak tegnieke (ensiem byvoegings [E], koue maserasie [CM], verlengde dopkontak [PM] en ‘n kombinasie van koue maserasie en verlengde dopkontak [CM+PM]) is vergelyk ten opsigte van tannien en antiosianien ekstraksie. In 2008 en 2009 is twee kultivars (Cabernet Sauvignon en Shiraz) in twee verskillende klimatologiese areas gepars. Hierdie areas is geklassifiseer in die Winklerskaal as ‘n IV (Plaisir de Merle) en ‘n III (Morgenster). Om die effek van die verskillende wynmaak tegnieke op die ekstraksie van antosianiene en tanniene te vergelyk, is hierdie twee kultivars by twee verskillende rypheidsgrade geoes. Die eerste oes was net voor kommersiële oes (LB) en die tweede oes het net na kommersiële oes (HB) plaasgevind. Die 2009 Shiraz wyn is organolepties beoordeel om die effek van die verskillende wynmaak tegnieke op die wyn se mondgevoel te vergelyk. Die resultate van hierdie studie toon beduidende verskille in die fenoliese samestelling van die wyne. Dit is gevind dat die warmer area hoër tannien konsentrasies het as die koeler area. In 2008 het die CM+PM die meeste tanniene uit die Cabernet Sauvignon geëkstraheer by LB en die CM by HB in die warmer area. Die CM het in die koeler area meer tanniene geëkstraheer by beide die LB en HB rypheidsgrade. In 2009 het PM die meeste tanniene geëkstraheer by LB terwyl E die meeste tanniene geëkstraheer in die warmer area. In die koeler area het CM+PM die meeste tanniene geëkstraheer, terwyl geen van die behandelings ‘n effek gehad het by HB. Die meeste antosianien konsentrasie was in die koeler area gevind as in die warmer area. In beide 2008 (LB en HB) en 2009 (LB) het CM die meeste antosianiene geëkstraheer, terwyl geen behandeling ‘n effek gehad het by HB. Twee van die mees populêre tannien analitiese metodes is die BSA (bovine serum albumien) en die MCP (metielsellulose presipitasie) metodes. Die BSA metode is ‘n baie meer ingewikkelde metode waarvoor drie keer meer reagense gebruik word as vir die MCP metode. Maar waar die MCP net tanniene ontleed, ontleed die BSA metode tanniene, monomere (MP), klein polimeriese pigmente (SPP) en groot polimeriese pigmente (LPP). Dit help indien daar gekyk wil word na die evolusie van polimeriese pigmente. In hierdie studie is bevind dat daar ‘n redelike korrelasie (R2 – 0.88) tussen die BSA en MCP metode bestaan. Die herhaalbaarheid van die metodes het redelike kontroversie veroorsaak, waar sommige navorsers bevind het dat die BSA metode nie so herhaalbaar is soos eers bevind is nie. Die MCP metode het ’n praktiese voordeel aangesien dit in die helfde van die tyd van die BSA metode uitgevoer kan word. Dit het ‘n groot impak indien ‘n groot hoeveelheid monsters ontleed moet word. Die fenoliese samestelling en mondgevoel word sterk beïnvloed deur die klimatologiese area. In die warmer area was die effek van tannien konsentrasie op mondgevoel kleiner as in die koeler area. Die wyn van ryper druiwe het meer harder, verdowingseffek en bitter nasmaak gehad as by die wyn van groener druiwe. Die ensiem behandeling was meer geassossieerd met droë mond gevoel.
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Suvac, Serghei. "Ensaios com alternativos de madeira e taninos enológicos em vinho tinto. Efeitos na composição química e análise sensorial." Master's thesis, ISA, 2013. http://hdl.handle.net/10400.5/6493.
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Mestrado em Viticultura e Enologia - Instituto Superior de Agronomia / Faculdade de Ciências e Tecnologia. Universidade do PortoThe aim of this study is to evaluate the effects of oak alternatives in red wine. The alternatives have been used: tannins of control enological tannin, tannin and extract of oak wood "Dialog", used doses were minimum, average and maximum. After one month aging in 10L bottles chemical composition and sensory analysis were evaluated. At the beginning of evaluation general analysis of wine were determined (% vol, pH, SO2, sugar, etc.) and analyzes which linked to phenolic compounds, (intensity and color tone, total anthocyanins, coloured anthocyanins, ionization index, polymeric pigments, total pigments, polymerization index, index of total polyphenols, total phenols, and CIELab analyze of color. Analyses showed that there are no major differences between samples, only the wine with concentration of oak extract 0.225 g/l have slightly higher values of total phenols, total polyphenols index and total anthocyanins. In terms of sensory analysis, in the tasting panel found no major differences in the overall assessment parameter, aroma and taste balance, some differences were found in wine with oak extract dose 0.225 g/l.
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Lucas, Ronaldo Carlos. "Características nutricionais e fatores antinutricionais na fermentação ruminal in vitro de espécies arbóreo-arbustivas nativas e exóticas em área de Caatinga no Sertão de Pernambuco." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/64/64134/tde-26092012-111917/.
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A vegetação nativa do sertão nordestino é rica em espécies forrageiras nos estratos herbáceo, arbustivo e arbóreo. Estudos têm revelado que acima de 70% das espécies botânicas da caatinga participam significativamente da composição da dieta dos ruminantes domésticos. Estrategicamente, as espécies lenhosas são fundamentais no contexto de produção e disponibilidade de forragem no semiárido Nordestino. Objetivou-se com este trabalho: (1) Quantificar a composição química bromatológica e os compostos fenólicos de leguminosas nativas; (2) Avaliar as características de degradação ruminal e o efeito biológico de plantas taniníferas baseado na técnica de produção de gás in vitro (bioensaio); (3) Estudar os efeitos de dietas constituídas de forrageiras nativas na síntese de nitrogênio microbiano in vitro com marcador 15N e nos parâmetros de fermentação pela técnica de produção gases. Os resultados quanto à composição química e quantificação dos compostos fenólicos das leguminosas taniníferas catingueira (Caesalpinea bracteosa), aroeira (Astronian urundeuva), leucena (Leucaena leucocephala), jureminha (Desmanthus virgatus) e do capim buffel (Cenchrus ciliaris L.) são apresentados. Estas plantas também foram avaliadas por incubação in vitro, juntamente com feno de alfafa (Medicago sativa) como controle, para avaliar o incremento de gás devido à adição de polietilenoglicol (PEG). As leguminosas apresentaram na produção de gases menor atividade biológica dos taninos com adição do PEG, sendo positivos os coeficientes de correlações entre o incremento na produção de gases na presença do PEG e os parâmetros de produção de metano, demonstrando o potencial destas plantas taniníferas para reduzir a produção de metano ruminal, com destaque para leucena e aroeira, com elevado teor proteic e menores teores de fibras. No capitulo 4 são apresentados os resultados do ensaio in vitro para avaliar os efeitos de dietas constituídas de forrageiras nativas da região de Caatinga do NE Brasileiro na síntese de nitrogênio microbiano, utilizando o marcador 15N; e os parâmetros de fermentação pela técnica in vitro de produção gases. As dietas foram constituídas com as espécies catingueira (Caesalpinea bracteosa), aroeira (Astronian urundeuva), leucena (Leucaena leucocephala), jureminha (Desmanthus virgatus) e capim buffel (Cenchrus ciliaris L.), oriundas de três coletas (Agosto de 2008, Março de 2009 e Agosto de 2009). Dois níveis (50% e 30%) de plantas foram usadas em cada dieta simulando sistema CBL (Caatinga, Capim buffel + Leucena). Em geral, as dietas experimentais apresentaram em sua composição química, elevado teor de proteína bruta baixa concentração de compostos fenólicos. Quando estas dietas foram avaliadas através da técnica de produção de gás in vitro, as dietas mostraram redução na emissão de metano, e os parâmetros fermentativos sugeriram que houve mudanças nas rotas de fermentação das dietas. O uso de leguminosas nativas da caatinga pode ser uma alternativa na região semi-árida, principalmente, oferecendo características favoráveis como: valor nutricional, potencial produtivo e rusticidade. No entanto, a viabilidade, no ponto de vista nutricional pode limitar o uso de algumas espécies, devido à alta concentração de compostos fenólicos, principalmente os taninos condensados.The native vegetation of Northeastern region of Brazil is rich in forage species in the herbaceous, shrubby and arboreal strata. Studies have revealed that over 70% of botanical species from the caatinga participate significantly in the composition of the diet of domestic ruminants. Strategically, the woody species are fundamental in the context of production and availability of forage in such semi-arid area. The objectives of this work were: (1) to quantify the chemical composition and the phenolic compounds of native legumes form the semi-arid region of Pernambuco; (2) to assess the rumen degradation characteristics and biological effect of such tanninferous plants based on in vitro gas production technique (bioassay); (3) to study the effects of diets consist of the native forage on microbial nitrogen synthesis in vitro using 15N as marker and the parameters of fermentation by gas production technique. Chapter 4 presented the results of the in vitro test to assess the effects of diets consist of native forage of Caatinga region of Brazilian NE upon the synthesis of microbial nitrogen using the 15N as tracer, and fermentation parameters. Diets were formed by the species catingueira (Caesalpinea bracteosa), aroeira (Astronian urundeuva), leucena (Leucaena leucocephala), jureminha (Desmanthus virgatus) e capim buffel (Cenchrus ciliaris L.), from three collections (August 2008, March 2009- August 2009). Two levels (50 and 30%) of the plants were used in each diet simulating CBL system (Caatinga + Buffel Grass + Leucena). In general, the experimental diets presented in their chemical composition high crude protein content and low concentration of phenolic compounds. When these diets were evaluated by the in vitro gas production technique, diets showed reduction of methane emission, and the fermentative parameters suggested that there have been changes of fermentation routes of diets. The use of legumes native to caatinga may be an alternative in the semi-arid region, primarily by offering favorable characteristics as: nutritional value, productive potential and homeliness. However, the viability, in nutritional point of view the use of some species can be limiting, because of high concentration of phenolic compounds in particular the condensed tannins.
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Pechamat, Laurent. "Impacts de l'oxygène sur les évolutions chimiques et sensorielles du vin rouge." Thesis, Bordeaux, 2014. http://www.theses.fr/2014BORD0355/document.
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L’impact de l’oxygène a été suivi pendant 4 mois pour 3 vins de Merlot d’IPT 61, 63 et 76. Le vin d’IPT 63 était enrichi par une fraction d’ellagitannins de bois de chêne. Les vins ont été ajustés à pH 3, pH 3,5 et pH 4. Pour chaque pH, des doses de 0, 2, 8 et 20 mg/L d’oxygène ont été fournies à l’aide d’oxygène pur, puis des analyses mensuelles ont permis de suivre les évolutions chimiques et sensorielles des vins afin d’apprécier les impacts liés à l’oxygène.Les résultats montrent pour tous les vins, que la consommation de l’oxygène augmente de 2 à 4 fois plus lorsque l’on passe de pH 3 à pH 4. La présence d’ellagitannins dans le vin favorise la consommation d’oxygène, ainsi qu’en solutions modèles hydro-alcooliques de fractions phénoliques. Les teneurs en tannins totaux sont globalement stables. Les polymérisations analysées par la méthode DMACH se produisent majoritairement à pH 3 et pH 3,5 pour le vin d’IPT 61, alors que les différences liées à l’oxygène sont moins fortes sur le vin d’IPT 76. Pour le vin d’IPT 63 + ellagitannins, les polymérisations sont favorisées à pH 3. Les teneurs en anthocyanes totales et moléculaires montrent généralement des diminutions en fonction du temps, de l’oxygène ainsi que de l’acidité. Pour le vin d’IPT 76 et d’IPT 63 + ellagitannins, les anthocyanes se polymérisent davantage aux tannins polymères en présence d’oxygène, et à pH plus acide. 13 pyranoanthocyanes ont pu être identifiées et quantifiées, dont deux molécules de pyranomalvidine-di-(épi)catéchine quantifiées pour la première fois dans des vins rouges secs qui peuvent être considérées comme des marqueurs en lien avec l'apport en oxygène subi par le vin. Les analyses sensorielles réalisées sur le vin d’IPT 76 en fonction des pH montrent que l’amertume est plus forte à pH 4, et pH 3,5 dans une moindre mesure. Cette tendance se retrouve aussi sur la perception d’oxydation.Pour les deux autres vins, d’IPT 61 et d’IPT 63 enrichi par une fraction d’ellagitannins, l’intensité du fruité diminue globalement avec les doses d’oxygène, tandis que le caractère oxydé est bien corrélée avec les doses croissantes d’oxygène, ainsi que l’amertume dans une moindre mesureThe impact of oxygen has been followed-up during 4 months for 3 Merlot red wines of TPI 61, 63 anf 76. The wine of TPI 63 was supplied by a fraction of ellagitannin from oak wood. Wines have been adjusted at pH 3, pH 3.5 and pH 4. For each pH, quantities of 0, 2, 8 and 20 mg/L of pure oxygen have been added. Then, anlyses have been performed each month to follow the wine chemical and sensory evolutions in order to determine the impact of oxygen.For all wines, the results show that oxygen consumption is 2 to 4 fold higher from pH 3 to pH 4. The presence of ellagitannin in wine favors oxygen consumption, as well as in wine model solutions with phenolic fractions. Total tannin contents remain quite constant. Polymerisations analysed by DMACH index occur mainly at pH 3 and pH 3.5 for the wine of TPI 61, while the differences related to oxygen are less important in the wine of TPI 76. For the wine of TPI 63 + ellagitannins, polymerisations are favored at pH 3. Total and molecular anthocyanins contents generally show a decrease according to time, oxygen and acidity. For the wines of TPI 76 and TPI 63 + ellagitannins, the anthocyanins polymerise more to polymers of tannins with oxygen and acidity. 13 pyranoanthocyanins have been identified et quantified. Among them, two molecules of pyranomalvidin-di-(epi)catechin quantified for the first time in a dry red wine. They can be considerated as marker related to oxygen amount consumed by wine. Sensory analyses realized on wine of TPI 76 according to pH show that bitterness is higher at pH 4, and pH 3.5 in a lesser extent. There is the same trend with the oxidation perception.For the two other wines, of TPI 61 and 63 with ellagitannin fraction, fruity intensity globally decreases with oxygen quantities, while the oxidized parameter is well related to increasing oxygen amounts, as wella s bitterness in a lesser extent
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Gonzalez, Rojas Alvaro. "Effect of plant growth regulator applications on phenolic quality of red grape berry skin and wine Vitis vinifera L., cvs Cabernet Sauvignon and Carmenère." Thesis, Bordeaux 2, 2012. http://www.theses.fr/2012BOR21920/document.
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La composition phénolique du vin rouge détermine fortement sa qualité: couleur, goût, texture et la plupart des bienfaits pour la santé. Les conditions ambiantes de la vigne modulent l'équilibre hormonal endogène et l'expression de gènes qui contrôlent la voie de synthèse des composés flavonoïdes, en déterminant la composition phénolique finale du raisin. Même s'ils ont été étudiés, les effets des applications des régulateurs de croissance végétale sur l'équilibre hormonal endogène et la qualité du raisin, les effets de ces substances sur la composition et la qualité du vin sont pauvrement documentés. Le traitement des raisins destinés à la vinification avec des régulateurs de croissance végétale est un outil potentiel pour modifier la qualité des raisins et du vin rouge. Ce projet de thèse a pour objectif d’étudier l’impact d'applications de régulateur de croissance végétale sur la composition phénolique des raisins de Vitis vinifera L. cépages Cabernet Sauvignon et Carménère. L’acide abscissique, l’acide indole-3-acétique et l'acide 2-chloroethilphosphonique ont été appliqués à divers stades phénologiques du raisin, doses et conditions environnementales: Les essais ont été menés à Maipo et Cachapoal au Chili et à Bordeaux en France, dans des vignobles commerciaux et expérimentaux ainsi que sur des plantes cultivées en pots. Il a été examiné l'effet de ces traitements sur le contenu interne d'hormones, sur l'expression de gènes structuraux et régulateurs de la synthèse de composés flavonoides et sur la qualité des raisins, en particulier la composition phénolique de sa pellicule. De plus, des vinifications ont été réalisées à partir de raisins traités pour déterminer l'effet des traitements sur la composition chimique et phénolique du vin, ainsi que sur des attributs qualitatifs tels que les arômes et la texture des vins, jugés par un panel d'évaluation sensoriellePhenolic composition strongly determines red wine quality: color, taste, texture and most health benefits. Vineyard environmental conditions modulate endogenous hormonal balance and gene expression which control the flavonoid biosynthetic pathway leading to final grape phenolic composition. Even when the effects of plant growth regulator applications on grape endogenous hormonal balance and quality have been studied, the effect of these substances on wine composition and quality is poorly documented. The treatment of wine grapes with plant growth regulators is a potential tool in order to modify red wine phenolic composition and quality. This thesis project describes six experiments on plant growth regulator applications on developing grapes of Vitis vinifera L., cvs Cabernet Sauvignon and Carménère. Abscisic acid, Indole-3-acetic acid and 2-chloroethylphosphonic acid were applied in different phenological stages, doses and environmental conditions: Maipo and Cachapoal regions in Chile and Bordeaux region in France, commercial and experimental vineyards and plants in containers. The effect on changes in the internal hormonal content, expression of flavonoid biosynthetic and regulatory genes and grape quality, in particular grape skin phenolic composition were examined. In addition, winemaking was performed in order to assess the effect of treatments on wine chemical and phenolic composition and on wine aroma and texture attributes judged by a sensory panel
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Reeves, Savanah Gail. "Condensed tannin characterization with FT-ICR MALDI mass spectrometry and separation with saw-tooth gradient HPLC." Miami University / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=miami1591185101154831.
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Silva, Jefferson Luiz Grangeiro da. "Analise critica das correlações e parametros fundamentais em sistemas de agitação." [s.n.], 2002. http://repositorio.unicamp.br/jspui/handle/REPOSIP/267607.
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Orientadores: Jose Roberto Nunhez, Efraim CekinskiTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia Quimica
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Resumo: O projeto atual de agitadores químicos é essencialmente dependente de relações empíricas, visto que as leis fisicas que regem os fenômenos envolvidos nos processos químicos são expressões cuja solução analítica é ainda desconhecida. Devido a isto, trabalhos experimentais como os compilados por Nagata, (1975), Oldshue, (1983), Sano e Usui, (1985) e Tatterson, (1991) são utilizados como principais referências para o cálculo do consumo de energia destes sistemas de agitação. Este projeto de pesquisa tem por objetivo principal compilar na literatura as principais correlações e trabalhos que são utilizados como base para o cálculo convencional de agitadores utilizados na indústria química, de forma que sejam definidas as melhores correlações através da análise dos parâmetros fundamentais (relações geométricas) no cálculo do Número de Potência, Número de Reynolds, Número de Froude, Número de Fluxo, Número de Mistura e o consumo de Potência de impelidores para os sistemas de agitação. A análise dos parâmetros geométricos será particularmente importante para estágios iniciais de projetos de plantas químicas, quando são necessárias informações da geometria, tanto do tanque como do impelidor (agitador) e, principalmente, do consumo de energia específica destes equipamentos para se estimar o consumo de energia da planta. Os resultados analisados através da utilização das correlações empíricas serão comparados com o Software comercial VISIMIX. Adicionalmente o L-CFD, laboratório de Fluído Dinâmica Computacional, já desenvolveu diversos projetos que analisam a otimização de projeto de reatores de mistura utilizando a Fluido Dinâmica Computacional e este projeto contribui por complementar o conhecimento deste grupo de pesquisa sobre o projeto de agitadores através de cálculos convencionais
Abstract: The design of impellers to date depends on empirical relations, since the physical laws that describe the phenomena involved in the chemical processes are expressions whose analytical solution is still unknown. Experimental works as those compiled by Nagata, (1975), Oldshue, (1983), Sano and Usui, (1985) and Tatterson, (1991) are used as references for energy consumption estimation in these systems. The compilation of the main correlations and the works in the literature that are used as a base to the conventional way to ca1culate the main impellers used in the chemical industry is the main objective of this research; which will allow one to select the better correlations it is carried on by the analysis of fundamental paramenters (geometric relations) for the ca1culation of Power Number, Reynolds Number, Froude Number, Flow Number, Mixing Number and the Power consumption of impellers in these systems. The analysis of the geometrical parameters will be particularly important to the early stages in the design of chemical plants, when informations ofthe tank and ofthe impeller (agitator) geometries and, mainly,about the specific energy consumption of these equipments are needed. The results are analysed using empirical correlations in the literature and are compared with the commercial software VISIMIX. Additionaly the L-CFD, Computational Fluid Dynamics laboratory, has already developed several projetcs to analyse the optimization project of mixing tanks, through the use of Computational Fluid Dynamics. This project contributes to complement the knowledge of this group of research on the design of mixing impellers, through the use of conventional methods
Doutorado
Desenvolvimento de Processos Químicos
Mestre em Engenharia Química
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Soares, Junia Rosa. "Think Tanks: organização sistêmica de conhecimentos relevantes a política pública no Brasil." Universidade do Estado de Santa Catarina, 2009. http://tede.udesc.br/handle/handle/188.
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Previous issue date: 2009-05-08Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
This master's thesis is an effort to systematize knowledge about the phenomena of think tanks - relevant actors of the public policy universe - through a theoretical-descriptive study, with the use of literature search. The think tanks, research organizations and advice on public policy, are widely studied in other countries, but in Brazil the phenomenon is not relevant elucidation. It is demonstrate the growing of a worldwide interconnection network between think tanks, and the increase of international studies on these organizations, while in Brazil the phenomenon remains allusive. As a result, this study purposes are to give visibility to the phenomena, adapting the traditional foreign literature to the Brazilian reality; to provide means to identify the influence exerted by these organizations in national policy making, structuring a comprehensive theoretical basis; and to allow the understanding and recognition, on empirical reality, of think tanks in action in Brazil. Its practical relevance are supported by the explanation of the phenomena, its context in the global and national landscape, and the analysis of this interest´s groups influence on the production of public policies in Brazil ‒ which is currently restricted in the national academic scene to a few scholars with specific focus: it is expected that more people understand what a think tank is and what is its activity. The study is conducted from the perspective of policy analysis and model group, focusing on the role of think tanks in the public policy cycle. Hence, as a study´s result, it is propose an expression, in Portuguese, to replace the use of expression think tank, and a concept that is appropriate to the reality of the phenomena in Brazil. There is also presented a classification of organizations, mapped to support the study as examples. Among the findings, it is consider the significant need to encourage studies about public policy, appropriate to the country s reality, since public policy is consider a "middle range theory", in a sense of a study field still being constructed that, however, directly influences the daily life ‒ and future ‒ of people
Esta dissertação de mestrado representa um esforço de sistematização do conhecimento sobre o fenômeno dos think tanks ‒ atores relevantes do universo da política pública ‒ e sua adequação à realidade nacional, por meio de um estudo teórico-descritivo e uso de pesquisa bibliográfica. Os think tanks, organizações de pesquisa e aconselhamento em políticas públicas, são amplamente estudados em outros países, mas no Brasil o fenômeno não encontra correspondente elucidação. É demonstrada a crescente interligação mundial em rede entre think tanks e o aumento de estudos internacionais sobre essas organizações, enquanto no Brasil o fenômeno permanece alusivo. Como conseqüência, este estudo objetiva conferir visibilidade ao fenômeno, adequando a literatura estrangeira tradicional à realidade brasileira; prover meios para identificação da influência exercida por essas organizações no processo nacional de policy making; estruturar uma base teórica abrangente, que permita a compreensão e o reconhecimento, na realidade empírica, de think tanks em atuação no Brasil. Sua relevância prática reside no esclarecimento do fenômeno, sua contextualização no panorama mundial e nacional e na análise dessa forma de atuação de grupos de interesse sobre a produção de políticas públicas no Brasil ‒ que se restringe atualmente, no cenário acadêmico nacional, a poucos estudiosos com enfoques específicos: espera-se que mais pessoas entendam o que são e o que fazem os think tanks. O estudo é conduzido sobre a ótica da policy analysis e o modelo de grupo, focalizando a atuação dos think tanks no ciclo da política pública. Como resultado do estudo é feita a proposição de uma expressão em português em substituição ao uso do termo think tank e de um conceito apropriado à realidade do fenômeno no Brasil. Apresenta-se ainda uma tipologia de classificação dessas organizações, na qual são enquadradas àquelas mapeadas no estudo, a título de exemplificação. Entre as conclusões obtidas, destaca-se como significativa a necessidade de fomentar estudos sobre a política pública, adequados à realidade do país, uma vez que se trata de uma middle range theory , isto é, um campo de estudo ainda em construção que, contudo influencia diretamente o cotidiano e o futuro ‒ das pessoas em geral
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Ahmed, Hameed, and Ye Xiaohong. "The Role Scenarios of EU in the World of 2020 : a Comparative Perspective Between European and US based Think Tanks." Thesis, Linköping University, Department of Management and Economics, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-2874.
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This paper aims to tell out a “what next” question of the future scenarios that the European Union (EU), as a security community, is most likely to face in its development as a world power up to the year 2020. The EU is a rapidly evolving concept consolidating with comprehensive internal and external dimension and implication. Key concepts such as power, security, global governance and international order have been used as the underlying themes that will determine the course of the future. In this context Neo-realism, Constructivism and Security Community’s model have been used as the theoretical framework to examine how EU’s role in the international system can be analyzed in the conceptual framework of security. This is followed by a discussion on the concurrent ESDP instruments initiated by the new Constitution as a key momentum of security community buildup internally. To broaden understanding and to get a variety of perspectives, research reports and policy papers of some transatlantic think tanks were analyzed. While multi- perspectives of some prominent think tanks were reviewed, analyzed and discussed, the opinions of policy makers at the United Nations Head Quarters, New York, were also explored to reflect on how EU’s role in international affairs is being perceived in the corridors of power, both concurrently and futuristically. These views were consequently augmented by attending General Assembly and Security Council sessions during September 2004 to January 2005 to observe what sorts of power and influence EU exerts in the contemporary international system and what potential it has for the future. Based on these findings, the paper identified three scenarios that EU is most likely to face in its efforts to be an international power. The paper concluded with the understanding that EU has vision and potential to be a power in the international system futuristically, which can be further enhanced if EU enhances its military capabilities and improves its power perception in the eyes of various stakeholders. However since the international system is volatile, its future role cannot be predicted with accuracy, only alternative options can be identified. EU’s roles are dependent upon the degree of success of the integration process internally and the structural interaction with other major actors in the international system. Whereas EU may enhance its role up to the year 2020, it may not enter into a power conflict with other major powers. However it will play its role comparable with the magnitude of its size, power and influence to manage the international system, futuristically in an improvised way.
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Pircher, M. "The effects of weld-induced imperfections on the stability of axially loaded steel silos /." View thesis View thesis, 2000. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20030506.145720/index.html.
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Gaillard, Mathilde. "Contribution à la caractérisation des think tanks américains à travers l'étude de leur discours et de leur culture professionnelle." Thesis, Sorbonne université, 2019. http://www.theses.fr/2019SORUL092.
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Cette thèse porte sur la caractérisation du milieu des think tanks aux États-Unis à travers l’étude de leur discours et de leur culture professionnelle dans le cadre d’une recherche en anglais de spécialité. L’objectif est de démontrer que ce milieu hétérogène peut être envisagé comme un milieu spécialisé, caractérisé par un discours et une culture spécifiques qui répondent aux impératifs de son objectif d’influence. Dans une première partie, nous exposons les fondements épistémologiques de la thèse : nous présentons les défis soulevés par l’étude de cet objet du point de vue de la recherche en anglais de spécialité, et proposons une approche de sa spécialisation fondée sur sa raison d’être. Une définition opératoire est élaborée, et le cadre méthodologique de la thèse à la croisée de l’histoire institutionnelle et politique des États-Unis, de l’ethnographie, de l’analyse du discours et de la pragmatique est exposé. La deuxième partie porte sur les modalités notamment culturelles de la spécialisation ; nous montrons comment le milieu s’est structuré en réponse à un besoin d’expertise dans la société américaine, et s’organise autour de missions, pratiques, valeurs et genres communs. Une topographie de ces genres est proposée pour éclairer les modalités communicationnelles de la stratégie programmatique des think tanks. La troisième partie explore les modalités discursives de cette stratégie dans deux genres, les blogs et les policy briefs, pris individuellement, puis en vis-à-vis ; l’analyse des modules péritextuels, structurels et interpersonnels révèle un ensemble de choix argumentatifs qui reflètent les enjeux de captation et de crédibilité de l’intervention des expertsThis dissertation aims to characterize the milieu of think tanks in the United States through their discourse and professional culture from the French point of view of research on English for Specific Purposes. We contend that this heterogeneous milieu may nonetheless be considered a specialized milieu, as its discourse and its culture are shaped by its programmatic aim. Part 1 presents the epistemological foundations of our research: it sheds light on the theoretical challenges raised by the study of think tanks in the framework of English for Specific Purposes and offers an approach to specialization based on its raison d’être. We suggest a working definition for the milieu and explain the methodology for this study situated at the crossroads between US institutional and political history, ethnography, the French approach to discourse analysis, and pragmatics. Part 2 focuses on the cultural manifestations of think tanks’ programmatic aim; it shows how the milieu has crystallized in response to a need for expertise in US society and is characterized by similar aims, practices, values and genres. A topography of these genres is offered to cast light on the milieu’s discursive strategy. Part 3 explores the discursive manifestations of this strategy and concentrates on two genres, blog posts and policy briefs, which are studied first individually and then jointly. The analysis of peritextual, structural and interpersonal modules reveals the argumentative choices experts make to remain credible and attractive in the marketplace of ideas
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Retaureau, Ghislain J. "Detection of Surface Corrosion by Ultrasonic Backscattering." Thesis, Georgia Institute of Technology, 2006. http://hdl.handle.net/1853/11498.
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Corrosion often occurs in the inner aluminum lining of the HB-53 helicopter external fuel tank, resulting in fuel leaks. This project centers on developing an in-situ ultrasonic inspection technique to detect corroded areas inside the fuel tank. Due to the complexity of the composite structure of the tank, the ultrasonic inspection is carried out from inside the tank using a monostatic backscattering technique. The backscattered field contains information related to the insonified surface properties (surface roughness scales). Numerical predictions are implemented with a simplified model of backscattered intensity (Ogilvy, 1991). Experimental results are obtained on artificially corroded plates, and on the actual fuel tank of the HB-53 helicopter. Signal processing techniques (Envelope Correlation and Inverse Technique) are used to detect corroded surfaces with data obtained with a focused 10 MHz pulsed transducer.
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Šmidtaitė, Rasa. "Netiesinių statistikų taikymas atsitiktinių vektorių pasiskirstymo tankių vertinime." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2008. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2008~D_20080811_151338-22521.
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Statistikoje ir jos taikyme vienas dažniausiai sprendžiamų uždavinių yra daugiamačių tankių vertinimas.Tankių vertinimas skirstomas į parametrinį ir neparametrinį vertinimą. Parametriniame vertinime daroma prielaida, kad tankio funkcija f, apibūdinanti duomenis yi, kai i kinta nuo 1 iki n, priklauso tam tikrai gan siaurai funkcijų šeimai f(•;θ), kuri priklauso nuo nedidelio kiekio parametrų θ=(θ1, θ2, …, θk). Tankis, apskaičiuojamas pagal parametrinį vertinimą, gaunamas iš pradžių apskaičiavus parametro θ įvertį θ0 ir f0=f(•;θ). Toks traktavimas statistiniu požiūriu yra labai efektyvus, tačiau jeigu nei vienas šeimos f(•;θ) narys nėra artimas funkcijai f, rezultatai gali būti gauti labai netikslūs.
Neparametriniam tankio vertinimui jokios parametrinės prielaidos apie f nėra reikalingos, tačiau vietoj to daromos kitos prielaidos, pavyzdžiui, apie funkcijos f tolydumą arba, kad f yra integruojama. Tankio funkcijos forma yra nustatoma iš turimų duomenų.Turint dideles imtis, tankis f gali būti apskaičiuotas pakankamai tiksliai.
Šiuolaikinėje duomenų analizėje naudojama daugybė neparametrinių metodų, skirtų daugiamačių atsitiktinių dydžių pasiskirstymo tankio statistiniam vertinimui. Ypač plačiai paplitę branduoliniai įvertiniai, populiarūs ir splaininiai bei pusiau parametriniai algoritmai. Taikant daugumą populiarių neparametrinio įvertinimo procedūrų praktikoje susiduriama su jų parametrų optimalaus parinkimo problema. Branduolinių įvertinių konstrukcijos svarbiausiu... [toliau žr. visą tekstą]Most algorithms work properly if the probability densities of the multivariate vectors are known. Unfortunately, in reality these densities are usually not available, and parametric or non-parametric estimation of the densities becomes critically needed. In parametric estimation one assumes that the density f underlying the data yi where i varies from 1 to n, belongs to some rather restricted family of functions f(•;θ) indexed by a small number of parameters θ=(θ1, θ2, …, θk). An example is the family of multivariate normal densities which is parameterized by the mean vector and the covariance matrix. A density estimate in the parametric approach is obtained by computing from the data an estimate θ0 of θ and setting f0=f(•;θ). Such an approach is statistically and computationally very efficient but can lead poor results if none of the family members f(•;θ) is close to f. In nonparametric density estimation no parametric assumptions about f are made and one assumes instead that f, for example, has some smoothness properties (e.g. two continuous derivatives) or that it is square integrable. The shape of the density estimate is determined by the data and, in principle, given enough data, arbitrary densities f can be estimated accurately. Most popular methods are the kernel estimator based on local smoothing of the data. Quite popular are histospline, semiparametric and projection pursuit algorithms. While constructing various probability density estimation methods the most... [to full text]
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Soares, Luiz Alberto Lira. "Obtenção de comprimidos contendo alto teor de produto seco por aspersão de Maytenus ilicifolia Mart. ex. Reissek - Celastraceae : desenvolvimento tecnológico de produtos intermediários e final." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2002. http://hdl.handle.net/10183/147612.
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As folhas de Maytenus ilicifolia são amplamente utilizadas na medicina tradicional brasileira no tratamento de distúrbios gástricos. Após a comprovação de sua eficácia e segurança, a referida droga vegetal tem sido objeto de diversos estudos com a finalidade de estabelecer parâmetros para o controle de qualidade. Neste sentido, foi avaliada a viabilidade do desenvolvimento de um método analítico fundamentado na precipitação de taninos empregando povidona como agente seqüestrante. Os dados revelaram que a remoção de taninos em solução é satisfatória quando empregada a povidona insolúvel. Foi observado que o polímero também é capaz de precipitar outros polifenóis além dos taninos presentes na solução extrativa de M. ilicifolia. Entretanto, as interações do polímero ocorrem preferencialmente com os derivados catéquicos. Adicionalmente foi desenvolvido e validado um método por CLAE para separação e quantificação de catequina e epicatequina em produtos derivados de M. ilicifolia. Não foram detectadas variações nas áreas dos picos ou nos tempos de retenção durante os ensaios de avaliação do método, sugerindo que as condições cromatográficas empregadas são satisfatórias. Para viabilizar a preparação de produto seco por aspersäo (PSA) em torre de secagem piloto munida de aspersor rotatório, foi necessária a concentração da solução extrativa. A operação foi realizada sob pressão reduzida até alcangar um teor de sólidos mínimo de 15 % (m/m). A operação de secagem da solução concentrada apresentou rendimento de 90 % e o PSA obtido apresentou partículas esféricas, com superfície rugosa e tamanho médio de 22,5 um. A caracterização tecnológica do material revelou que as suas propriedades tecnológicas, tais como, densidades, compressibilidade e fluxo, foram incrementadas quando comparados com o produto preparado em equipamento de bancada. Entretanto, estas modificações não foram suficientes para viabilizar sua compressão direta. Assim sendo, o material foi compactado em máquina de comprimir e em rolos para produção de grânulos. A análise do perfil compressional do PSA e dos granulados, empregando o modelo de Heckel, revelou que o aumento da força de compactação durante a operação de granulação foi o principal responsável pela diminuição no potencial de deformação plástica dos grânulos. Como conseqüência, a recompressão dos grânulos em máquina de comprimir originou compactos com dureza inferior àqueles preparados diretamente do complexo farmacêutico. Enquanto o PSA apresentou reduzida tendência ao rearranjo particular nos estágios iniciais da compressão, os grânulos sofreram intensa reacomodação decorrente da fragmentação e formação de novas pontes, assumindo comportamento plástico em pressões de compressão mais elevadas. Por fim, comprimidos contendo teor elevado de granulado de PSA foram preparados utilizando como adjuvantes a celulose microcristalina, o dióxido de silício coloidal e a croscarmelose sódica. A influência da concentração de dióxido de silício coloidal e croscarmelose sódica na formulação, sobre as respostas de dureza, friabilidade e tempo de desintegração dos comprimidos foram avaliadas através de um Desenho Composto Central. Os dados experimentais foram analisados estatisticamente, utilizando os modelos matemáticos para gerar superfícies de resposta. Os resultados indicaram que a concentração de dióxido de silício coloidal foi inversamente relacionada às respostas de dureza e friabilidade, enquanto que a concentração de croscarmelose sódica foi a principal responsável pela redução no tempo de desintegração. As condições ótimas foram selecionadas através da sobreposição dos gráficos, buscando uma formulação com menor tempo de desintegração, menor friabilidade e o máximo de dureza. A formulação eleita deve conter 1,2 % (m/m) de dióxido de silício coloidal e 5,0 % (m/m) de croscarmelose sódica. Nestas condições os comprimidos apresentaram dureza de 107,9 N, friabilidade de 0,56 % (m/m) e desintegração máxima em 6,8 min.The leaves of Maytenus ilicifolia are widely used within the traditional Brazilian medicine due to its properties against gastric disorders. Once proved its efficacy and safety, this vegetable drug has been the object of several studies to establish the basis for quality control. Taking this into consideration, it was necessary to evaluate the feasibility of developing an analytical method based on the precipitation of tannins, by using polyvinylpyrrolidone for precipitation. The data have shown that the removal of the tannins in solution was successful when insoluble polyvinylpyrrolidone was used. Hereby it was observed that the polymer has also the ability to precipitate other polyphenols besides the tannins from the aqueous solution of M. ilicifolia. However, the interactions of the polymer happened mostly with the condensed tannins. Further it was developed and also validated an HPLC method to separate and quantify catechin and epicatechin present in aqueous extracts of M. ilicifolia. No significant variations of peak areas or retention times were observed when the evaluation of the method was executed, showing satisfactory chromatographic conditions. In order to make a spray dried extract (SDE) using a spray-drier fitted out with a rotary atomizer, it was necessary to concentrate the extractive solution. This operation had been performed under reduced pressure until a content of 15 % solids was achieved. The drying of the concentrated solution lead to a yield of 90 %, and the obtained SDE presented spherical particles, which have a rough surface and a mean particle size of 22,51.1.m. The technological characterization of the SDE showed that attributes such as densities (tap and bulk), compressibility and flow, have been improved when compared to the product prepared using a mini spray-drier supplied with a pneumatic nozzle. However, these modifications were not enough to enable its direct compression. Therefore, the material was compacted to produce granules after slugging or roller compaction, The compressional analysis of the SDE and each granule batch using the Heckel equation proved that the increase in the compaction force once processing the granulation was the main factor to reduce the material's ability to undergo plastic deformation. Consequently, the recompression of the granules in a tablet press led to compacts with a lower crushing strength compared to that prepared by direct compression of the pharmaceutical complex. Whereas the SDE has shown a reduced tendency to particle rearrangement at early stage of compression, the granules showed an intense fragmentation and rebound behavior, presenting a plastic behavior when higher compaction pressures were used. Finally, tablets containing a high dose of granulated spray dried extract of M. ilicifolia were prepared by using the following excipients: microcrystalline cellulose (filler/binder), colloidal silicon dioxide (glidant and moisture adsorber) and cross-linked carboxymethylcellulose (disintegrant). The influences of colloidal silicon dioxide (CSD) and cross-linked carboxymethylcellulose (CMC-Na) on the tablet hardness, disintegration time and friability were evaluated by a central composite design. The data were analyzed statistically and mathematical models were used to create response surfaces. The results have indicated that the concentration of CSD shows an inverse relation to the responses of hardness and friability, while the concentration of CMC-Na was the most important factor that caused a reduction of the time necessary for total disintegration. The optimal conditions for processing were chosen by the overlapping of graphics, taking into consideration that the formulation should present a minimum of disintegration time, lower friability and a maximum of hardness. Thus, it was found that the best formulation should have a content of 1.2% (w/w) of CSD and 5.0% (w/w) of CMC-Na. Regarding these conditions, the tablets have shown a hardness of 107.9 N, friability of 0.56% (w/w) and a disintegration time of 6.8 min.
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Sjöberg, Edvin. "Herrväskan Primus : produktutveckling för Ödins Garveri." Thesis, Högskolan på Gotland, Institutionen för humaniora och samhällsvetenskap, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:hgo:diva-1124.
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I denna rapport redovisas ett examensarbete av Edvin Sjöberg utfört i samarbete med Ödins Garveri i Visby. Projektets mål har varit att ta fram ett tillverkningsunderlag för en ny axelremsväska i läder för män. Väskan är konstruerad för att vara materialbesparande, hållbar och elegant. Faktainsamlingen ger en översikt av de vanligaste sätten att bereda och garva skinn, sömnadsteknik och tillverkningsteknik. Målgrupps- och marknadsanalys har varit vägledande för utformningen av slutprodukten och stort fokus har lagts på modell- och skissarbete. Slutprodukten är en herrväska med mycket hög materialkvalité, formgivning inspirerad av klassiskt herrmode och med väl planlagda och genomtänka detaljer.This report presents a thesis of Edvin Sjöberg performed in collaboration with Ödins Tannery in Visby. The project's goal has been to develop a manufacturing base for a new leather shoulder bag for men. The bag is designed to be easy to produce without material loss, to be durable and stylish. The essay provides an overview of the most common ways to prepare and tan hides, sewing techniques and manufacturing technologies. Audience and market analysis has aided the design of the finished product and focus has been placed on model and sketch work. The end product is a bag made from high quality materials, design inspired by classic men's fashion and with well-planned and thoughtful details.
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Chvostek, Ida-Maria. "Treacherous Words : How Climate Change Conspiracy Sceptics use Conceptual Metaphors to Extinguish our Future." Thesis, Umeå universitet, Institutionen för språkstudier, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-161233.
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This study examined the metaphors used in contemporary American conservative discourse between October 2018 and March 2019, focusing on material published by conservative think tanks (CTTs) and tweets made by Republican senators in relation to climate change. For the CTTs, a domain-specific corpus (36,388 words) was compiled and a smaller corpus (3967 words) was assembled based on 135 tweets. These datasets showed that conspiracy scepticism was the most common type of scepticism used to discredit climate change data, scientists and environmental policies. In addition, the datasets indicate that conservative agents frequently used metaphors of WAR, RELIGION, HEALTH, BUILDING, JOURNEY, WATER and PRODUCT to convey negative frames. These domains linked to the conceptual key LIFE IS A STRUGGLE FOR SURVIVAL and were presented in a moral context. In response to these findings it is suggested that the scientific community incorporate emotional language, metaphors and moral values when communicating environmental issues.
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Zahradníček, Pavel. "Analýza ztráty integrity nádrže pomocí MKP." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2014. http://www.nusl.cz/ntk/nusl-231310.
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This diploma thesis deals with the storage tanks for petroleum products. The introductory part is devoted to the distribution of the storage tanks, associated standards and maintenance of tanks. Another part goes about the analysis of real case of a tank collapse. There was created a 3D model of the collapsed tank according to the measured values in the tank. It was created a tank shell model by drawing documentation and analysis of the results of the accident investigation using the finite element method (FEM) by program ANSYS. Calculation was performed on the buckling resistance of the structure according to ČSN EN 1993-1-6 and its comparison with the results of FEM analysis in ANSYS. The aim of the study was to find the critical condition of the structure and determination of the critical internal underpressure and overpressure. At the end there were determined measures for safe operation and settings of the valve members.
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Hedmark, Eva. "Conservation Genetics of Scandinavian Wolverines." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Universitetsbiblioteket [distributör], 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-6636.
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Mattos, Fernando Preusser de. "O Brasil no debate estratégico franco-alemão : uma análise do discurso dos principais think tank da Alemanha e da França a respeito da inserção internacional do Brasil (2003-2014)." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2016. http://hdl.handle.net/10183/139360.
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As iniciativas de política externa, de defesa e de segurança que compõem a inserção internacional do Brasil no período 2003-2014 repercutiram nas relações do país com as grandes potências, suscitando uma intensa produção discursiva que busca traduzir o significado da recente inserção internacional do país e informar estratégias que possam dar conta das demandas de um novo relacionamento com o Brasil. Nesse contexto, há um conjunto de instituições de importância crescente, cujas práticas, representações e discursos precedem, informam e pretendem influenciar os processos de definição de agenda, formulação de política e tomada de decisão no âmbito das políticas externa, de defesa e de segurança, sobretudo das grandes potências: os think tanks, instituições dedicadas à produção e à articulação de conhecimentos voltados a políticas públicas de âmbito doméstico e internacional. Assim, o problema de pesquisa que orienta o trabalho refere-se ao seguinte questionamento: como os principais think tanks que buscam influenciar as formulações estratégicas para as políticas externa, de defesa e de segurança na Alemanha e na França têm representado a inserção internacional recente do Brasil e o papel desempenhado pelo país no seu entorno estratégico? O objetivo geral da dissertação é, portanto, fornecer uma análise do discurso acerca da inserção internacional recente do Brasil (2003-2014) veiculado por publicações selecionadas dos quatro principais think tanks da Alemanha e da França. O trabalho lança mão da fundamentação teórico-metodológica da análise de discurso pósestruturalista e busca sustentar a tese de que é possível depreender da análise das publicações selecionadas um conjunto de discursos básicos que estruturam as principais representações desses atores sobre a recente inserção internacional do Brasil, dentre os quais se destacam o discurso da inovação diplomática, do ineditismo e do soft power socioeconômico dos governos Lula; o discurso do reformismo moderado, que associa as identidades de “parceiro responsável” e “líder pragmático” ao Brasil, em contraposição ao “radicalismo” e ao “populismo” da Venezuela; e o discurso da pretensão de projeção global de poder.Brazil’s foreign, defense and security policies between 2003-2014 affected its relations with the great powers, raising a variety of discourses that seek to translate the country’s recent international insertion and inform strategies that may cope with the demands of a new relationship with Brazil. In this context, there is a set of increasingly important institutions whose practices, representations and discourses precede, inform and seek to influence agenda setting, as well as policy and decision making processes in the fields of foreign, defense and security policies, especially of great powers: think tanks, institutions whose activities are mainly focused on producing and engaging knowledge on domestic and international public policies. The research problem orienting this monograph is, thus, the following: how do the most important foreign, defense and security policy think tanks in Germany and France represent Brazil’s recent international insertion and the role it plays in its regional surrounding area? The main aim of this project is, therefore, to offer an analysis of the discourse on Brazil’s recent international insertion (2003-2014) produced and disseminated by four of the most important think tanks in Germany and France. The monograph adopts post-structuralist discourse analysis as its theoretical and methodological foundation and lays out the argument that the analysis of selected think tanks’ publications reveals a set of basic discourses structuring key-representations of identity attributed to Brazil. Among them, three basic discourses are highlighted throughout the discussion: Lula’s diplomatic innovation and socioeconomic soft power discourse; the moderate reformism discourse, which links the identities of a “responsible partner” and “pragmatic leader” to Brazil in opposition to the “radicalism” and “populism” attributed to Venezuela; last but not least, the discourse on Brazil’s alleged claim to global power projection.
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Yoshime, Luciana Tedesco. "Caracterização nutricional do Mangarito (Xanthosoma mafaffa Schott)." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/9/9131/tde-10112017-105442/.
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O mangarito (Xanthosoma mafaffa Schott) ou mangará, é uma das espécies da família Araceae, e está entre inúmeras plantas rústicas encontradas nas regiões tropicais. A grande maioria dessas espécies são amiláceas, classificadas como calóricas e consideradas alimentos de subsistência, porém, não há muitos dados sobre esses alimentos não-convencionais, e o estudo deles, pode ter um papel importante na suplementação da alimentação de populações desnutridas e de baixo poder aquisitivo. Dessa forma, o presente trabalho teve como objetivos avaliar composição centesimal, fibras solúveis e insolúveis, minerais, α-tocoferol e o perfil de carotenóides, no mangarito cru (CR) e cozido (CZ). Foram avaliadas cinco colheitas do mangarito provenientes de um único cultivar no interior do Estado de São Paulo entre os anos de 2005 e 2007. Sua composição centesimal foi de aproximadamente 71,93% de umidade; 1,23% de cinzas; 1,43% de lipídeos e 2,11 % de proteínas e houve perdas estatisticamente significativas após o cozimento. Dessa forma, o valor calórico total do mangarito foi de aproximadamente 103,11 kcal/100g. Os valores de fibras alimentares foram de 3,5% (CR) e 3,62% (CZ), não havendo diferença estatisticamente significativa entre eles. O resultado da análise de minerais revelou valores elevados de cálcio (CR=290,60 e CZ=330,0mg/100g), ferro (CR=3,90 e CZ=1,8mg/100g), potássio (CR=1408,70 e CZ=1300,4mg/100g) e zinco (CR=4,2 e CZ=5,20mg/100g). Foram encontrados 72,04µg/g de α-tocoferol e a perda pelo cozimento foi de 14,28%. Os carotenóides encontrados nas amostras foram α-caroteno (1,26µg/g), ζ-caroteno (3,41µg/g) e traços de mutatocromo e micronona e cozimento ocasionou perda de 20 a 66% para α-caroteno e de 6 a 60% para ζ-caroteno. Apesar da identificação de um carotenóide com atividade pró-vitamínica (α-caroteno), as quantidades encontradas no mangarito não foram suficientes para que o alimento recebesse a designação de fonte dessa vitamina.Tannia (Xanthosoma mafaffa Schott) is characterized as a rustic plant found in tropical countries such as Brazil. Vegetables are sources of carotenoids that could be used in prevention of vitamin A deficiency. Considering the generally higher cost of animal foods, the provitamin A from vegetables should be considered as an expressive and easily obtainable source of vitamin A in developing countries Although tannia are popularly c1assified as a source of carotenoids, studies on its chemical composition are rare. Thus, because of the absence of the cultivation, tannia as well as others non-traditional vegetables may become extinct and still be classified as potential source of nutrients. Therefore the goal of the present study is to characterize the chemical composition and carotenoids profile of raw (RT) and cooked tannia (CT). It was analyzed 5 different crops of tannia from the countryside of Sao Paulo state harvested between years 2005 and 2007. When compared to RT, CT presented less (p<0.05) water, protein and fat. Regarding the total amount of energy, RT presented values between 95,12 to 143,60 Kcal/100g while CT presented values between 100,96 to 142,91 Kcal/100 g. In both RT and CT it was found great quantity of calcium (RT =290,60 mg/100 g; CT =330,0 mg/100 g), iron (RT =3,90 mg/100 g; CT = 1,8 mg/100 g), potassium (RT = 1408,70 mg/100 g; CT = 1300,4 mg/100 g) and zinc (RT = 4,2 mg/100 g; CT = 5,20 mg/100 g). Moreover, in RT it was found 72,04 µg of α-tocopherol while CT presented 14,28 % less. It was found α-carotene and ζ-carotene, mutatochrome and micronone in both RT and CT; however CT presented smaller (p<0.05) concentration of these carotenoids. In conclusion, tannia can not be considered a good source of provitamin A because of the small concentration of α-carotene. Although the great quantity of important minerals, more studies are necessary to elucidate their bioavailability in tannia.
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Tavano, Matteo. "Seismic response of tank-fluid systems: state of the art review and dynamic buckling analysis of a steel tank with the added mass method." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2012. http://amslaurea.unibo.it/3006/.
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Diaz, Calderon Alvaro Emilio, and Ventocilla Brigitte Carolina Meniz. "Evaluación estructural de reservorios apoyados de concreto armado en Lima Metropolitana considerando la norma ACI 350-06 y las normativas peruanas." Bachelor's thesis, Universidad Peruana de Ciencias Aplicadas (UPC), 2019. http://hdl.handle.net/10757/626005.
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En la presente tesis se ha desarrollado la evaluación estructural de cinco reservorios circulares del tipo apoyado, construidos entre los años 1977 y 1997, ubicados en zonas de alto riesgo sísmico en Lima Metropolitana y ubicados en suelos medianamente rígidos, con el objetivo de evidenciar si estas estructuras continúan conservando un diseño sísmico adecuado en base a los requerimientos sísmicos actuales, y por ende si serán capaces de resistir un evento sísmico severo y continuar con el servicio.
Para poder modelar y determinar la respuesta de los se reservorios se empleó el modelo equivalente de Housner, obteniendo así la masa impulsiva y convectiva, modelado en el programa SAP2000 con ayuda de las normas ACI 350.3-06 y E.030. En cuanto a la determinación de las fuerzas resistentes, para poder realizar la evaluación estructural correspondiente, se utilizó la norma peruana E.060-2009 Concreto Armado, con la cual se obtuvo dichas fuerzas y se realizaron las verificaciones estructurales.
Con respecto a los resultados de las verificaciones realizadas, se observó que los reservorios en estudio no mantienen un diseño estructural adecuado en cuanto a las solicitaciones sísmicas actuales. Estas deficiencias se plasman en déficit de refuerzo horizontal por corte en muros, cuantía mínima vertical por corte en muros, refuerzo en la base del muro por momento tangencial, armadura requerida en la viga collarín, y refuerzo en el extremo de la cúpula por tracción radial; por lo que estas estructuras, ante la presencia de un evento sísmico severo, se encuentran expuestas a presentar fallas estructurales.In the present thesis has been carried out the structural assessment of five round ground concrete tanks, built between 1977 and 1997, and located in high seismic risk areas in Lima Metropolitana in moderately rigid soils, with the objective of demonstrating if these structures still preserve an adequate structural design base on the current standards and consequently, if they will be able to withstand a severe seismic event and, hence, continue with their service. In order to model and determine the response of the tanks, the Housner’s rigid equivalent model was used, obtaining this way the impulsive and convective masses, which were modeled in the software SAP2000 with the ACI 350.3-06 standard and the E.030 Peruvian standard. Regarding on the determination of the resistant forces, in order to carry out the corresponding structural evaluation, the Peruvian standard “Concreto Armado E.060” was utilized. With regard to the results of the verifications carried out, it was observed that the reservoirs under study do not maintain an adequate structural design in terms of the current seismic solicitations. These deficiencies are reflected in horizontal reinforcement deficit by shear force on the walls, minimum amount of vertical rebar by shear on the walls, reinforcement in the base of the wall by tangential bending moment, rebar required in the beam by radial tensile force, and rebar in the end of the dome by radial traction; so these structures, in the presence of a severe seismic event, are exposed to structural failures.
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Kovář, Jiří. "Návrh regulace tlaku a průtoku v soustavě čerpadlo-nádrž." Doctoral thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2014. http://www.nusl.cz/ntk/nusl-234215.
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The proposed work is dealing with control design of pump-tank system as part of broader water-supply network with using of mechatronic approach. The main goal of this work is design of control for regulation of flow, pressure and water level in tank in water-supply network of Vsetin city. This water-supply network consists of various components, such as pumps, pipes, valves, tanks etc. For the purpose of the control design is necessary to create a model of pipe network. The solution of hydraulic analysis is obtained by using the gradient method, which was implemented into the software solution named ADAM. The results of hydraulic analysis are compared with measured values of flow and pressure and difference of these values has to be minimalized by proposed calibration and verification process. The calibration and verification process was implemented in software ADAM. The essence of design control is formulating control problem as optimization problem. Therefore the output of higher control layer is set of procedural rules, which determines for example speed of pumps in time etc. Results were verified by using for this purpose developed computational server (ADAM Server).
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Tanistrová, Lucie. "Hodnocení technického stavu a posouzení rizik vodojemů." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2020. http://www.nusl.cz/ntk/nusl-409711.
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The diploma thesis is primarily focused on risk analysis and risk assessment of drinking water supply systems. In the research, the issues of risk analysis and risk assessment considering world and Czech Republic, were summarized. Software WaterRisk, which was developed by BUT Brno, was used for risk assessment of a particular water supply system in Třebom. Next, the thesis is aimed on technical audit of water tanks. There was a research of technical audit in the world and in the Czech Republic. Also assessment of technical audit of a particular water tank, which had to be anonymized, using software TEAwater is described in detail. Two conditions were considered: before and after reconstruction.
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Wenzel, Moritz. "Development of a Metamaterial-Based Foundation System for the Seismic Protection of Fuel Storage Tanks." Doctoral thesis, Università degli studi di Trento, 2020. http://hdl.handle.net/11572/256685.
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Metamaterials are typically described as materials with ’unusual’ wave propagation properties. Originally developed for electromagnetic waves, these materials have also spread into the field of acoustic wave guiding and cloaking, with the most relevant of these ’unusual’ properties, being the so called band-gap phenomenon. A band-gap signifies a frequency region where elastic waves cannot propagate through the material, which in principle, could be used to protect buildings from earthquakes. Based on this, two relevant concepts have been proposed in the field of seismic engineering, namely: metabarriers, and metamaterial-based foundations.
This thesis deals with the development of the Metafoundation, a metamaterial-based foundation system for the seismic protection of fuel storage tanks against excessive base shear and pipeline rupture. Note that storage tanks have proven to be highly sensitive to earthquakes, can trigger sever economic and environmental consequences in case of failure and were therefore chosen as a superstructure for this study. Furthermore, when tanks are protected with traditional base isolation systems, the resulting horizontal displacements, during seismic action, may become excessively large and subsequently damage connected pipelines. A novel system to protect both, tank and pipeline, could significantly augment the overall safety of industrial plants.
With the tank as the primary structure of interest in mind, the Metafoundation was conceived as a locally resonant metamaterial with a band gap encompassing the tanks critical eigenfrequency. The initial design comprised a continuous concrete matrix with embedded resonators and rubber inclusions, which was later reinvented to be a column based structure with steel springs for resonator suspension. After investigating the band-gap phenomenon, a parametric study of the system specifications showed that the horizontal stiffness of the overall foundation is crucial to its functionality, while the superstructure turned out to be non-negligible when tuning the resonators.
Furthermore, storage tanks are commonly connected to pipeline system, which can be damaged by the interaction between tank and pipeline during seismic events. Due to the complex and nonlinear response of pipeline systems, the coupled tank-pipeline behaviour becomes increasingly difficult to represent through numerical models, which lead to the experimental study of a foundation-tank-pipeline setup. Under the aid of a hybrid simulation, only the pipeline needed to be represented via a physical substructure, while both tank and Metafoundation were modelled as numerical substrucutres and coupled to the pipeline. The results showed that the foundation can effectively reduce the stresses in the tank and, at the same time, limit the displacements imposed on the pipeline.
Leading up on this, an optimization algorithm was developed in the frequency domain, under the consideration of superstructure and ground motion spectrum. The advantages of optimizing in the frequency domain were on the one hand the reduction of computational effort, and on the other hand the consideration of the stochastic nature of the earthquake. Based on this, two different performance indices, investigating interstory drifts and energy dissipation, revealed that neither superstructure nor ground motion can be disregarded when designing a metamaterial-based foundation. Moreover, a 4 m tall optimized foundation, designed to remain elastic when verified with a response spectrum analysis at a return period of 2475 years (according to NTC 2018), reduced the tanks base shear on average by 30%. These results indicated that the foundation was feasible and functional in terms of construction practices and dynamic response, yet unpractical from an economic point of view.
In order to tackle the issue of reducing the uneconomic system size, a negative stiffness mechanism was invented and implemented into the foundation as a periodic structure. This mechanism, based on a local instability, amplified the metamaterial like properties and thereby enhanced the overall system performance. Note that due to the considered instability, the device exerted a nonlinear force-displacement relationship, which had the interesting effect of reducing the band-gap instead of increasing it. Furthermore, time history analyses demonstrated that with 50% of the maximum admissible negative stiffness, the foundation could be reduced to 1/3 of its original size, while maintaining its performance.
Last but not least, a study on wire ropes as resonator suspension was conducted. Their nonlinear behaviour was approximated with the Bouc Wen model, subsequently linearized by means of stochastic techniques and finally optimized with the algorithm developed earlier. The conclusion was that wire ropes could be used as a more realistic suspension mechanism, while maintaining the high damping values required by the optimized foundation layouts.
In sum, a metamaterial-based foundation system is developed and studied herein, with the main findings being: (i) a structure of this type is feasible under common construction practices; (ii) the shear stiffness of the system has a fundamental impact on its functionality; (iii) the superstructure cannot be neglected when studying metamaterial-based foundations; (iv) the complete coupled system can be tuned with an optimization algorithm based on calculations in the frequency domain; (v) an experimental study suggests that the system could be advantageous to connected pipelines; (vi) wire ropes may serve as resonator suspension; and (vii) a novel negative stiffness mechanism can effectively improve the system performance.
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Umbel, Matthew R. "Prediction of Turbulent Mixing at the Interface of Density Stratified, Shear Flows Using CFD." Ft. Belvoir : Defense Technical Information Center, 1998. http://handle.dtic.mil/100.2/ADA477048.
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Smith, Mark R. (Mark Robert). "Development of a method to measure protein in red wines : a survey of protein, mannan and tannin in Pinot noir wines." Thesis, 2010. http://hdl.handle.net/1957/17875.
Full textAbstract:
A number of methods to isolate and quantify protein in Pinot noir wines were evaluated. The combination of precipitation by acetone containing 10% w/v trichloroacetic acid followed by quantification with the Bradford assay, reported in yeast invertase equivalents yielded the most accurate results when compared to micro-Kjeldahl analysis. The technique was validated by dialysis and proteolysis experiments and was used to assay protein in 57 Pinot noir wines. These wines were found to contain protein concentrations ranging from 49 to 102 mg/L. The mannan and tannin content of the wines was also measured, but no correlation between protein content and concentrations of these components was found. The
presence of protein in red wines greater than 30 years old at concentrations typically found in white wines contradicts the notion that interactions with tannin severely reduce protein levels.Graduation date: 2011
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Oberholster, Anita. "Investigation of the chemical and sensory properties of red wine pigments." 2008. http://hdl.handle.net/2440/50452.
Full textAbstract:
Phenolic compounds play an important role in red wine colour, bitterness, astringency, as well as a range of other tactile or 'mouth-feel' characteristics. Progressive changes of phenolic compounds, initially extracted from grapes, occur during the storage and aging of red wines. The decrease of astringency occurring during wine aging has been considered as a result of mainly anthocyanin-flavanol condensation either directly or mediated by aldehydes. The contribution of these polymeric pigments formed during wine aging to the unique properties of red wine is an important question still unanswered. Experiments were conducted to synthesise polymeric pigments in model wine solutions under different conditions in the absence of acetaldehyde to provide material for chemical and sensory studies. Only small amounts of polymeric pigments were formed in these experiments confirming that direct polymerisation is a slow process. The low yield of polymers made it necessary to investigate the isolation of polymeric pigments directly from wine. A preparative fractionation protocol was developed to obtain fractions enriched in different red wine pigment combinations for further investigation. A HPLC method was also developed that separated the pigmented and non-pigmented polymers, as well as the monomeric anthocyanins, flavanols (monomeric to trimeric), flavonols and hydroxycinnamic acids from each other. A 6 month old and a 5 year old Shiraz wine were fractionated and further analysed by acid hydrolysis in the presence of a nucleophile, gel permeation chromatography (GPC) and by different mass spectrometry techniques. The various fractions isolated from the 6 month old wine contained combinations of pentameric to dimeric pigments, while those from the 5 year old wine contained pigments with an average degree of polymerisation (DP) of at least 11 but possibly up to 32. Experiments were conducted to determine the sensorial contribution of anthocyanins to wine as well as the effect of anthocyanin-flavanol polymerisation reactions taking place during maturation. It was shown that significant changes occur in the polymeric phenol composition and in the mean degree of polymerisation of Shiraz wine during aging. With aging more skin tannin were incorporated in the pigmented polymers and the percentage of galloylation in these polymers decreased. In order to describe the sensory attributes of the polymeric pigments a refined vocabulary, describing the astringent and other mouth-feel sensations elicited by dry red table wines representing different styles was developed and called the mouth-feel wheel. The developed mouth-feel wheel was used in a study to investigate the contribution of anthocyanins and anthocyanin-proanthocyanidin reaction products to the mouth-feel properties of red wine. Wines were made from both red and white grapes with and without pomace contact, as well as with and without anthocyanin addition to the white grapes. The white wine made like a red wine did not exhibit the same mouth-feel sensory attributes of a red wine: it was lower in viscosity, less particulate in nature and lower in intensity for the astringency descriptors fine emery, dry and grippy. The presence of anthocyanins during fermentation appeared to increase the intensity of astringency related terms. Treatments with added anthocyanins increased the amount of polymeric phenols to twice that when compared to treatments without added anthocyanins.http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1345103
Thesis (Ph.D.) - University of Adelaide, School of Agriculture, Food and Wine, 2008
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Mbongeni, Khanyile. "Nutritive value of Acacia species and response of pigs fed on incremental levels of Acacia tortilis leaf meal-based diets." Thesis, 2013. http://hdl.handle.net/10413/11285.
Full textAbstract:
The broad objective of the current study was to determine the response of pigs fed on incremental levels of Acacia tortilis leaf meal-based diet. Eight trees of each of the following five dominant leguminous leaf meals; A. tortilis, A. robusta, A. nilotica, A. nigrescens and A. xanthophloea, were individually hand harvested from the same grazing camp at Makhathini Research Station, Jozini, South Africa. The leaf meals were harvested between April and May 2013 after the rainy season at advance stage of maturity. Following the nutritive evaluation of Acacia species, A. tortilis was selected for the feeding experiment. Thirty finishing male F1 hybrid (Landrace × Large White) pigs with an initial weight of 60.6 (s.d. = 0.94) kg were randomly allotted to six dietary treatments containing 0, 50, 100, 150, 200, 250 g/kg DM inclusion levels of A. tortilis leaf meal. Each treatment diet was offered ad libitum to five pigs in individual pens for 21 days.
Average daily feed intake (ADFI), body weight, average daily gain (ADG) and gain: feed ratio was measured every week. Blood was collected at the end of the experimental period for the determination of nutritionally-related blood metabolites and activity of Aspartate aminotransferase (AST), alanine amino transferase (ALT) and alkaline phosphatase (ALP). Following a feed withdrawal period of 12 hours, pigs with a mean body weight of 80 ± 15 kg were slaughtered, eviscerated for the collection of livers, kidneys, lungs and heart from each pig and weighed individually using a digital scale.
Acacia tortilis and A. xanthophloea leaf meals had the highest CP and fat content (P < 0.01) among all the Acacia species. The NDF and ADF varied significantly across Acacia species (P < 0.05). Acacia robusta had significantly the highest levels of non-structural carbohydrates, whilst A. tortilis had the lowest. The concentration of proanthocynidins varied across the species. Acacia tortilis had significantly high levels (23 g/kg DM) of phosphorus compared to the other species. Acacia nilotica had the highest (P < 0.001) concentration of iron, but it had the lowest (P < 0.01) level of manganese.
There was a quadratic decrease in both ADFI and ADG (P < 0.001) with as A. tortilis leaf meal increased. The gain: feed ratio was linearly reduced (P < 0.001) with incremental levels of A. tortilis leaf meal in the diets. Serum concentrations of Fe, AST and ALP increased quadratically (P < 0.01) as A. tortilis leaf meal increased. There was a linear increase in ALT activity with increased leaf meal inclusion. Hepatosomatic index (HSI: liver weight/ body weight x 100), scaled kidney weight and scaled heart weight increased linearly (P < 0.001) as A. tortilis leaf meal increased. There was, however, a quadratic increase in the relative weight of lungs (P < 0.001) as leaf meal increased. In conclusion, the piecewise regression (brocken-stick) NLIN procedure (SAS, 2008; SASA institute Inc.) showed that A. tortilis leaf meal can be included in finisher pig diet up to 150 g/kg DM of feed without negatively affecting growth performance, nutritionally-related blood metabolites, liver enzymes and internal organs of pigs.M.Sc.Agric. University of KwaZulu-Natal, Pietermaritzburg 2013.
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Γρηγορίου, Βασίλειος. "Μελέτη συστημάτων σεισμικής προστασίας κρυογενικών δεξαμενών υγροποιημένου φυσικού αερίου με χρήση προσομοιωμάτων πεπερασμένων στοιχείων." 2006. http://nemertes.lis.upatras.gr/jspui/handle/10889/97.
Full textAbstract:
Στην εργασία αυτή διερευνάται η δυναμική απόκριση κρυογενικών δεξαμενών υγροποιημένου φυσικού αερίου υποκείμενων σε δράση σεισμού. Επειδή οι εν λόγω δεξαμενές αποτελούν κρίσιμα στοιχεία για τη λειτουργία ενός συστήματος διανομής φυσικού αερίου και επειδή ενδεχόμενη καταστροφική αστοχία τους θα είχε δυσβάστακτες κοινωνικές επιπτώσεις, ιδιαίτερα αυστηρές απαιτήσεις ισχύουν σχετικά με τον αντισεισμικό σχεδιασμό τους. Για το σχεδιασμό έναντι σεισμού προδιαγράφονται συνήθως ένα σεισμικό γεγονός με μέση περίοδο επαναφοράς της τάξης των 500 ετών, κατά το οποίο οι δεξαμενές απαιτείται να παραμένουν πλήρως λειτουργικές, και ένα σεισμικό γεγονός με μέση περίοδο επαναφοράς της τάξης των 5000 έως 10000 ετών, κατά το οποίο απαιτείται η εξασφάλιση της ασφαλούς διακοπής της λειτουργίας των δεξαμενών, ενώ ελάχιστη μόνο βλάβη είναι αποδεκτή στα δομικά στοιχεία τους. Οι εν λόγω δεξαμενές αποτελούνται από δύο κελύφη: Ένα εξωτερικό κυλινδρικό κέλυφος από προεντεταμένο σκυρόδεμα με θολωτή στέγη από οπλισμένο σκυρόδεμα και ένα εσωτερικό κέλυφος από κρυογενικό χάλυβα, εντός του οποίου αποθηκεύεται το προϊόν. Τα δύο κελύφη εδράζονται σε κοινή πλάκα βάσης. Στην εργασία αυτή εξετάζονται δύο εναλλακτικοί τρόποι έδρασης της πλάκας βάσης: α) απλή έδραση επί του διαμορφωμένου εδάφους και β) εφαρμογή κάποιας διάταξης σεισμικής μόνωσης επί της οποίας εδράζεται η πλάκα βάσης. Ειδικότερα, εξετάζονται τα ακόλουθα συστήματα σεισμικής μόνωσης: α) μόνωση με ελαστομεταλλικά εφέδρανα με ελαστομερές υψηλής απόσβεσης, β) μόνωση με ελαστομεταλλικά εφέδρανα με πυρήνα μόλυβδου, και γ) μόνωση με συνδυασμό γραμμικών ελαστομεταλλικών εφεδράνων και μη γραμμικών αποσβεστήρων. Στις ιδιαιτερότητες του δυναμικού προβλήματος περιλαμβάνονται, εκτός από την επίδραση της μόνωσης, η δυναμική αλληλεπίδραση των δύο κελυφών, η δυναμική αλληλεπίδραση υγρού-δεξαμενής, ο κυματισμός της ελεύθερης επιφάνειας του υγρού και η δυναμική αλληλεπίδραση εδάφους-δεξαμενής. Τα φαινόμενα αυτά λαμβάνονται υπόψη στην ανάλυση. Για τη μελέτη του κυματισμού της ελεύθερης επιφάνειας χρησιμοποιείται η γραμμική θεωρία κυμάτων. Για τη μελέτη της αλληλεπίδρασης υγρού-δεξαμενής εφαρμόζεται μια προσέγγιση τύπου Lagrange με χρήση πεπερασμένων στοιχείων υγρού. Για τη μελέτη της αλληλεπίδρασης εδάφους-δεξαμενής χρησιμοποιούνται συγκεντρωμένα στοιχεία δυσπαραμορφωσιμότητας και απόσβεσης. Επίσης, συγκεντρωμένα στοιχεία δυσπαραμορφωσιμότητας και απόσβεσης χρησιμοποιούνται για την προσομοίωση των συστημάτων μόνωσης. Τα χαρακτηριστικά των παραπάνω συγκεντρωμένων στοιχείων για την προσομοίωση της αλληλεπίδρασης εδάφους-δεξαμενής και των συστημάτων μόνωσης επιλέγονται με βάση ρεαλιστικές υποθέσεις για τις δυναμικές ιδιότητες του εδάφους θεμελίωσης και τα χαρακτηριστικά των συστημάτων μόνωσης. Δύο περιπτώσεις υφιστάμενων δεξαμενών υιοθετούνται προς ανάλυση. Οι δύο δεξαμενές διαφέρουν κυρίως ως προς τη χωρητικότητα και το λόγο ύψους προς ακτίνα. Και οι δύο δεξαμενές έχουν μελετηθεί και κατασκευαστεί χωρίς σεισμική μόνωση. Ένα συνθετικό επιταχυνσιογράφημα συμβατό με το φάσμα απόκρισης σχεδιασμού του Ευροκώδικα 8 για λόγο απόσβεσης 5%, για την αντίστοιχη κατηγορία εδάφους και για μέγιστη εδαφική επιτάχυνση λίγο μεγαλύτερη από αυτή για την οποία είναι σχεδιασμένες οι δεξαμενές περιγράφει τη διέγερση της κατασκευής στην οριζόντια διεύθυνση. Για την ανάλυση αναπτύσσεται ένα συνολικό παραμετρικό προσομοίωμα πεπερασμένων στοιχείων το οποίο περιλαμβάνει τα δύο κελύφη και το αποθηκευμένο υγρό. Στο προσομοίωμα συμπεριλαμβάνονται, ανάλογα με την περίπτωση που κάθε φορά αναλύεται, τα κατάλληλα στοιχεία για την προσομοίωση της αλληλεπίδρασης εδάφους-δεξαμενής ή των διαφόρων συστημάτων μόνωσης. Το προσομοίωμα χρησιμοποιείται για τον υπολογισμό των ιδιομορφών και την πραγματοποίηση δυναμικών γραμμικών και μη γραμμικών αναλύσεων με ολοκλήρωση στο πεδίο του χρόνου. Οι μη γραμμικές αναλύσεις είναι αναγκαίες για να ληφθεί υπόψη με ρεαλιστικό τρόπο η έντονα μη γραμμική συμπεριφορά των συστημάτων μόνωσης. Στην περίπτωση αυτή η μη γραμμικότητα είναι συγκεντρωμένη στα στοιχεία που προσομοιώνουν τη μόνωση ενώ για την υπόλοιπη κατασκευή γίνεται σε κάθε περίπτωση η παραδοχή γραμμικής συμπεριφοράς. Η παραδοχή αυτή προκύπτει από το γεγονός ότι για τις δεξαμενές αυτού του τύπου η αποδεκτή βλάβη, και επομένως μη γραμμικότητα στη συμπεριφορά, των δομικών στοιχείων είναι ελάχιστη. Παρουσιάζονται επιλεγμένα αποτελέσματα των αναλύσεων που πραγματοποιήθηκαν με χρήση του προσομοιώματος πεπερασμένων στοιχείων. Τα αποτελέσματα αυτά αναφέρονται κυρίως στην τέμνουσα βάσης, τη ροπή ανατροπής, τις οριζόντιες μετακινήσεις της κατασκευής σε διάφορες στάθμες και το ύψος του κυματισμού. Έμφαση δίνεται στην ποσοτικοποίηση της επίδρασης της αλληλεπίδρασης εδάφους-δεξαμενής και της σεισμική μόνωσης στα ανωτέρω μεγέθη. Η εργασία τελειώνει με γενικά συμπεράσματα.In the present work the dynamic response of liquefied natural gas cryogenic tanks subjected to earthquake action is investigated. Since these tanks are critical elements for the function of a natural gas distribution system and because a potential failure of them could lead to a major disaster, very severe requirements concerning the seismic design of these tanks are imposed. For the design against earthquake action two seismic events are generally considered: an event with a mean return period of 475 years during which the tanks are expected to remain fully operational and an event with a mean return period of the order of 5000 to 1000 years during which the safe shut down of the tanks is to be ensured, while minimum damage is accepted at the structural parts of the tanks. The type of tanks under consideration is constituted of two shells: an outer one made of prestressed concrete and an inner one made of cryogenic steel in which the product is stored. Both shells rest on a common base slab. In these work two alternative ways for the support of this slab are examined: a) the slab lays directly on the ground and b) the slab lays on a number of devices which provide seismic isolation. The following isolation systems are examined. a) high damping rubber bearings, b) lead core rubber bearings and c) low damping rubber bearing in conjunction with non-linear viscous dampers. The examined structural dynamic problem is characterised by certain particularities, besides the one of the implementation of an isolation system. The most important of them are sloshing of the free surface, dynamic fluid-structure interaction, dynamic soil-structure interaction and interaction between the inner and outer shell. These phenomena are taken into consideration in the preformed analyses. For the modeling of the free surface effect the realistic assumption of small wave height is made and the linear wave theory is employed. For the modeling of the fluid-structure interaction a Lagrangian approach is applied using finite elements for the modeling of the liquid and the solid domain. The soil-structure interaction is simulated by using concentrated stiffness and damping elements. Concentrated stiffness and damping elements are used for the modeling of the isolation systems as well. Two cases of existing tanks are adopted for analysis. The two tanks differ mainly in capacity and in the height to radius ratio. Both tanks are designed and constructed without seismic isolation. A global parametric finite element model is developed for the analyses. In this model the appropriate elements are incorporated for the modeling of the soil-structure interaction and the seismic isolation system, depending on the analysed case. The model is used for the calculation of eigenmodes and eigenfrequencies and for performing linear and non-linear transient analyses in time domain. Non-linear analyses are necessary in order for the highly non-linear behavior of the isolation devices to be properly simulated. In these cases the non-linearity is concentrated at the elements which simulate the seismic isolation system while the rest of the structure is considered elastic in all cases. This consideration is justified by the fact that for these tanks the acceptable damage, and consequently non-linearity in the behavior of the structural part, is minimum. Selective results of the performed analyses are presented. These results refer mainly to base shear forces, overturning moments, horizontal displacements at different levels of the tank and sloshing heights. Emphasis is on the quantification of the impact of the soil-structure interaction and the implementation of the examined seismic isolation systems.