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Ni Luh Putu Kris Monika Yanti, Sukrasno, Defri Rizaldy, and Arif Al Iman. "Profiling Secondary Metabolite of Fresh and Fermented Marigold Flowers and Butterfly Pea Flowers." Ad-Dawaa' Journal of Pharmaceutical Sciences 5, no. 2 (2022): 140–56. http://dx.doi.org/10.24252/djps.v5i2.37714.
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Communities in Bali use "Usada" as an essential plant for traditional medicine. Utilization of a plant as herbal medicine is based on the metabolites contained therein, such as in the marigold flower (Tagetes erecta L.) and butterfly pea flower (Clitoria ternatea L.). Identification of secondary metabolite profiles was carried out by aerobic fermentation. This study aims to identify the secondary metabolite profiles of untreated extracts compared to fermented extracts. Aerobic fermentation is carried out by stacking fresh samples. Extraction by percolation using n-hexane for marigold flowers and methanol for butterfly pea flowers. The extract profile was analyzed by densitometric thin-layer chromatography (TLC). Separation pattern, retention time, and m/z fragmentation with LC-MS/MS. Determined antioxidant activity using the DPPH method, calculating the levels of total phenol (TPC), total flavonoids (TFC), and total carotenoids in each sample. The TLC profiles of control extracts and marigold flower fermentation were similar, However, in the fermented pea flower extract, the intensity of the spots was thicker, and a new compound was formed from the TLC pattern. The TPC of the control and fermented extracts ranged from 0,816 - 1,154 g GAE/100 g extract, the TFC ranged from 0,067 - 0,610 g QE/100 g extract, and the highest total carotenoid was in the fermented marigold flower extract with levels of 215 mg/g simplicia. All control and fermented extracts had antioxidant activity ranging from 46,71 ppm-75,30 ppm. LC-MS/MS analysis showed five compounds from the fermented butterfly pea flower extract, while the control only identified one compound. The fermentation process for each extract experienced changes in the profile of secondary metabolites, starting from UV absorption, spot intensity, compound levels, types of compounds contained, and their antioxidant activity.
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Fortes, Paulo Jordão de Oliveira Cerqueira, Nelson Leal Alencar, Livio Cesar Cunha Nunes, et al. "CANNABIS THIN LAYER CHROMATOGRAPHY (TLC) CHEMO TYPING: HOW ACCURATE IS TLC COMPARED TO HIGH PRESSURE LIQUID CHROMATOGRAPHY?" Revista Contemporânea 3, no. 10 (2023): 19702–24. http://dx.doi.org/10.56083/rcv3n10-164.
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Problem: Brazilian Cannabis Associations (BCA) search for methods of reducing their costs and increasing their quality control of Cannabis spp. Oil and extracts. The significant difficulties presented by ABRACAM (Associação Brasileira Cannabis Medicinal) to UFPI professors were to standardize cannabinoids profiles of its plants and guarantee the same levels of cannabinoids on its artisanal extracts. ABRACAM has difficulty standardizing plant cannabinoids profiles due to Cannabis spp. Phenotypic plasticity, or the ability of Cannabis spp. to change attributes like the size of leaves, levels of secondary metabolites, stems structures, flowering period, among others. The second problem is that artisanal extract combines different production batches of non-tested extractions. It is necessary to quantify and qualify each cannabinoid and how much is on artisanal Cannabis production. Aiming to solve the ABRACAM production problem, UFPI and CANNAPI suggested manual testing practices to test cannabis and its derivates. This article results from validation tests performed at ABRACAM to determine how precise manual methods are to quantify cannabinoids. Methods: This research compared the cannabinoids results of an off-the-shelf Thin Layer Chromatography (TLC) cannabinoids profile test kit with the results of a High-Pressure Liquid Chromatography (HPLC). UFPI perfect the method from the vendor manual after running sixty quantifications from one sample. Results: The TLC error compared to HPLC was between approximately 0,5% and 1%. ABRACAM differentiated the Chemotype Profile of its High THC varieties and High CBD varieties. For High CBD variety, the outdoor cultivation proportionated greater cannabinoids levels. Conclusions: The comparison pointed that TLC may be used as testing equipment for this Brazilian Association due to its artisanal production license. This article concludes that precision is the key to medication production, and the TLC error may be accepted for artisanal production but not as a standard for precision. For scientific purposes, TLC is a strong hypothesis builder, and for agriculture, purposes may be a vital tool point the right time to harvest.
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Pratama, Mohammad Rizki Fadhil, Suratno Suratno, and Evi Mulyani. "Profile of Thin-Layer Chromatography and UV-Vis Spectrophotometry of Akar Kuning Stem Extract (Arcangelisia flava)." Borneo Journal of Pharmacy 1, no. 2 (2018): 72–76. http://dx.doi.org/10.33084/bjop.v1i2.367.
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This study aims to obtain the profile of Thin-Layer Chromatography (TLC) and Ultraviolet-Visible (UV-Vis) spectrophotometry from ethanol extract of akar kuning stems (Arcangelisia flava) from Central Kalimantan. The TLC method is used with the orientation phase of the combination of polar-non-polar solvents resulting from orientation, while ethanol is used as the solvent for UV-Vis spectrophotometers. TLC results showed the formation of 3 stains on a combination of polar solvents chloroform : methanol : water while in a non-polar solvent combination n-hexane : ethyl acetate did not show any stains. Comparison of retention factor (Rf) values show the best combination of polar solvents to separate stains at a ratio of 5 : 2 : 1, respectively. Separation in 2-dimensional TLC with polar solvents showed a similar pattern with 1-dimensional separation in the form of 3 stains. UV-Vis spectrophotometer results showed 4 main peaks with wavelength 227.2; 267.4; 345.2; and 425.3 nm, respectively. The profile of the peak formed is very similar to that shown by berberine, one of the main metabolites of akar kuning. TLC and UV-Vis spectrophotometers profiles obtained are expected to support further research using akar kuning stems, especially those from Central Kalimantan.
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Aswal, Manju, Shiv Prasad, Nishant Kumar, Veenesh Rajpoot, and Mohan Mondal. "The haemato-metabolic profile of high producing dairy cows during the transition period." Veterinarski arhiv 91, no. 3 (2021): 249–59. http://dx.doi.org/10.24099/vet.arhiv.1037.
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The objective of the present study was to assess the haematological [haemoglobin, packed cell volume (PCV), total erythrocyte count (TEC), erythrocytic indices, total leucocyte count (TLC) and differential leucocyte count (DLC)] changes and metabolic adjustment, viz. blood glucose, blood urea nitrogen (BUN), non-esterified fatty acid (NEFA) and cholesterol, in high-yielding Sahiwal and Karan Fries cows during the transition period. For this purpose, blood samples were collected from 8 pregnant Karan Fries crossbred cows and 8 pregnant Sahiwal cows on days -15, -7, 0 (the day of calving), +7 and +15 from calving, and used for estimation of haemoglobin, PCV, TLC, TEC, erythrocytic indices and DLC, and metabolic parameters, such as blood glucose, BUN, NEFA and cholesterol. It was found that haemoglobin concentrations, PCV and TEC increased significantly (P<0.01) up to the day of calving, and gradually decreased thereafter to reach pre-partum levels in the subsequent weeks post-calving. While the TLC, number of neutrophils and monocytes were found to increase (P<0.01), the number of lymphocytes decreased significantly (P<0.01) on the day of calving and then reached pre-partum levels after calving. No significant effect was observed on erythrocytic indices parameters. Blood glucose, BUN and NEFA levels showed an increasing trend (P<0.01) from 2 weeks pre-partum until the day of calving, and then decreased to the pre-partum level after calving. Unlike the other parameters, the plasma cholesterol level increased consistently from 2 weeks pre-partum to 2 weeks post-partum. In conclusion, the results of our study clearly show a definite pattern of the haematological and metabolic adjustment of high-producing dairy cows during the pre-partum to postpartum transition.
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VARUN, VARUN, SUMEET SHARMA, H. S. SANDHU, and NAVJOT S. GOSAL. "Haematological profile of subchronic oral toxicity of molybdenum in buffalo calves." Indian Journal of Animal Sciences 84, no. 5 (2014): 520–22. http://dx.doi.org/10.56093/ijans.v84i5.40664.
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An experimental trial was conducted to assess the influence of excess dietary molybdenum for prolonged period of time on haematological profile of healthy buffalo calves. Repeated oral administration of sodium molybdate @3 mg/kg/day for 90 consecutive days produced profound decline in the mean values of Hb, TLC, TEC, and MCHC in buffalo calves. It is suggested that exposure to excess dietary molybdenum should be avoided in buffalo calves.
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Hartanti, Dwi, and Jirapat Theeravit. "Ekstraksi Kayu Artocarpus lakoocha: Pengaruh Metode Dan Rasio Bahan Tumbuhan-Penyari Terhadap Rendemen Ekstraksi." PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) 15, no. 1 (2018): 50. http://dx.doi.org/10.30595/pharmacy.v15i1.2813.
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Artocarpus lakoocha Roxb. (Ma-haad) is a medicinal plant commonly used in Thai Traditional Medicine (TTM) for a wide array of indications. In this study, we compared yield of extraction and the Thin Layer Chromatography (TLC) profile of the heartwood of A. lakoocha extracted with the different methods and different material-solvent ratios. Extractions were carried out by maceration and reflux extraction with ethanol as the solvent, in material-solvent ratio of 1:5 and 1:3. The TLC profile was obtained from the separation of the extracts with methylene chloride/methanol (85:15) on silica gel F254 plate. The result exhibited that reflux extraction produced the highest yield of extraction and the material-solvent ratio of 1:3 generated more yield than that of 1:5. Nevertheless, the profile of TLC chromatogram of those extracts were similar.
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Tietel, Zipora. "Two-Dimensional Thin-Layer Chromatography as an Accessible, Low-Cost Tool for Lipid-Class Profile Screening." Separations 11, no. 6 (2024): 161. http://dx.doi.org/10.3390/separations11060161.
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The interest in lipid composition profiling is significantly increasing as research reveals the immense importance of lipids in medicine, plant science, food and agriculture. However, lipidomic analysis requires high-end specialty equipment. We used two-dimensional thin-layer chromatography (2D-TLC) as a readily available, low-cost tool for basic lipidomic profiling of lipid classes in algal samples in the models Chlamydomonas reinhardtii, Auxenochlorella protothecoides, and Euglena gracilis, validating lipid class identification using an LC-MS/MS analysis. Algal lipid extracts were separated on a 2D-TLC plate, and TLC analysis was followed by scraping individual TLC spots off the plate, and a subsequent liquid chromatography separation and tandem mass spectrometry (LC-MS/MS) analysis. For comparison, crude lipid extracts were also injected directly to the LC-MS/MS system. Lipid class annotation was achieved by a combination of accurate mass, retention time information, neutral loss and fragment ion analysis by MS2Analyzer, and by matching spectra to LipidBlast MS/MS library. Overall, we were able to identify 15 lipid classes, and to adequately profile the lipid classes in all three organisms. This TLC method is thus suggested as an accessible tool for lipid class profiling of algal, plant, and food lipids, alike, when a rapid and simple analysis is required, e.g., for screening purposes.
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Rafsanjanny, H., MAH Sarker, MZ Islam, et al. "Blood profile of lactating crossbred cows rearing at Bangladesh Agricultural University Dairy Farm." Bangladesh Journal of Animal Science 48, no. 2 (2020): 139–44. http://dx.doi.org/10.3329/bjas.v48i2.46768.
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This study aimed to analyze the hemato profile of crossbred lactating cows reared at Bangladesh Agricultural University (BAU) Dairy Farm. Thirty-two (32) healthy crossbred lactating cows of Holstein-Frisian (HF), Jersey (J), Sindhi Sahiwal (SS) and Red Chittagong (RCC) were selected for the study and divided into four (04) groups. The cows were allowed to feed and manage according to dairy farm own scheduled-ration. The collected blood samples were analyzed for hematological and biochemical parameters viz. Hemoglobin (Hb), Packed cell volume (PCV), Total leukocyte count (TLC), and Total erythrocyte count (TEC) and Serum urea, Glucose, Albumin, Calcium, and Phosphorus, respectively by using an automated chemistry analyzer. The results revealed that the hematological and biochemical parameters non-significantly (p>0.05) differed between the crossbred groups. From the experiment, the higher amount of Hb (11 g/dL) and PCV (40%) was found in HF and RCC cows, respectively. The TLC was higher in SS and RCC but the TEC was recorded stable along with a slight increment in RCC. As far as the biochemical parameters are concerned, the blood urea concentration was found higher (28 mg/dL) in HF and RCC lactating cows. The same pattern was observed in the case of albumin concentration. On the contrary, the lowest value of Calcium was found in RCC but the Phosphorus concentration showed a little higher in RCC. It can be concluded that the overall herd health was satisfactory based on the blood profile parameters.
 Bang. J. Anim. Sci. 2019. 48 (2): 139-144
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Lakshmi, R., K. Vijayakaran, D. T. Kaarthick, et al. "Haematological and Metabolic Profile Test of Subclinical Mastitis Affected Cross Bred Cattle." International Journal of Bio-resource and Stress Management 15, Feb, 2 (2024): 01–07. http://dx.doi.org/10.23910/1.2024.5044.
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The current research was conducted during May–October, 2023 aimed to assess the haemato-biochemical parameters in cattle affected with S. aureus affected subclinical mastitis. Mastitis in dairy cattle is the persistent, inflammatory reaction of the udder tissue. Financial losses due to mastitis occur in the case of both sub-clinically and clinically affected animals. Sub-clinical mastitis exhibits no clinical signs and mostly remains unnoticed by the farmer and can be detected if specific tests are performed in milk samples. Sub-clinical mastitis has an erosive effect on the economy of dairy farmers as it effect directly on the milk quality and quantity. In India prevalence of sub-clinical mastitis was found to be more in cows when compared to clinical mastitis. Blood samples were collected from the jugular vein of 10 healthy and 10 subclinical mastitis affected cattle. Blood samples were analyzed for Hb, PCV, TEC, TLC, while serum samples were examined for ALT, AST, ALP, Glucose, Total protein, Albumin, Cholesterol and Calcium. Haematology report showed significantly (p<0.01) decrease in Hb, PCV and TEC in subclinical affected cattle when compared to healthy animals. TLC was significantly increased in (p<0.01) subclinical affected animal than healthy animal. Biochemical estimate revealed significantly (p<0.05) higher average values of AST, ALP and Ca in subclinical mastitis affected animals compared to healthy animals. Glucose, total protein, albumin, cholesterol and ALT levels are significantly (p<0.05) decrease in subclinical mastitis affected animals when compared to healthy cows. Haemato-biochemical parameters can be used as important for pathological state of subclinical mastitis animals.
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Pandey, Pinky, Bharat Jha, and Anima Shrestha. "Cytological and Biochemical Profile of Cerebrospinal Fluid from Meningitis Patients." Annals of Clinical Chemistry and Laboratory Medicine 1, no. 1 (2015): 2–5. http://dx.doi.org/10.3126/acclm.v1i1.12305.
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BACKGROUND: The term Meningitis is used to describe an inflammatory infection of the membranes surrounding the brain and spinal cord, which occurs as either a primary disease or secondarily to disease in some other part of the body. The diagnosis is primarily confirmed by analyzing cerebrospinal fluid (CSF). Early diagnosis of the cause may be based on the cytological and biochemical parameters. Our objective was to determine the cytological and biochemical profile of CSF from meningitis patients. METHODS: In this cross sectional study, a total of 356 CSF specimens were collected from patients suspected of meningitis and processed microscopically and microbiologically by standard microbiological methods in Emergency Lab of Tribhuvan University Teaching Hospital (TUTH) Kathmandu, Nepal, over a period of six months, from March to August 2014 to determine cytological and biochemical parameters. RESULTS: Out of the 13 confirmed bacterial meningitis cases from 356 processed CSF samples, the mean value of total leukocytes count (TLC) was found to be 337.3 cells/mm3 with predominant neutrophils (73.8%). The mean value of glucose and protein was 28.8 mg/dL and 89.4 mg/dL respectively in the cases of bacterial meningitis. Among the three confirmed cases of fungal meningitis, the mean value of TLC was 11.7 cells/mm3 with lymphocytic predominance. In fungal meningitis glucose level was found to be normal (45.0 mg/dL) with slight increase in protein (48.7 mg/dL). CONCLUSIONS: Bacterial meningitis is generally characterized by increased TLC with predominance of neutrophils, decreased glucose and increased protein levels whereas fungal meningitis displays decreased TLC predominance of lymphocytes, normal or reduced glucose and slight increased protein level. DOI: http://dx.doi.org/10.3126/acclm.v1i1.12305 Ann. Clin. Chem. & Lab. Med. 1(1) 2015: 2-5
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Syarpin, Syarpin, and Wahyu Nugroho. "TLC Profile of Methanol Extrac of Diospyros confertiflora Stem Bark." Jurnal Ilmiah Kanderang Tingang 11, no. 2 (2020): 338–42. http://dx.doi.org/10.37304/jikt.v11i2.106.
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One of the large genus of plants growing in the Borneo forests is the genus Diospyros such as Diospyros siamang Bakh., Diospyros confertiflora (Hiern) Bakh., Diospyros areolata King and Gamble and Diospyros bornensis. Some of these plants are widely used by local people as medicinal purpose such as Diospyros borneensis as a medicine to treat panu by utilizing the bark and some other species have also been reported to have bioactivity as antioxidants, cosmetics, antidiabetes and hyperglukoma. In this study, Diospyros confertiflora bark was extracted by methanol. Then, the extract was evaluated using TLC to find out secondary metabolites content in Diospyros confertiflora. The TLC test showed that the methanol extract of diospyros confertiflora bark contains polar compounds and is predicted as a phenolate compound.
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Paramashivam, Meera Devi Sri, Ramasamy Duraisamy, Arul Vasu C, and Ilavarasan R. "A Comparative study on pytoconstitutents of Punica granatum flowers of Normal and Ornamental variety using TLC/HPTLC methods." Journal of Scientific and Innovative Research 5, no. 3 (2016): 87–91. http://dx.doi.org/10.31254/jsir.2016.5305.
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Punica granatum is a shrub that has a bushy appearance, have a good n umber of varieties, cultivated either as commercial crop for the production of pomegranates or as an ornamental crop for its beautiful flo wers. Among the ornamental crop of Punica granatum, the “Double flower” producing plant gains much significant value as it is used in Unani drug un der the name of Gulnar for treating many ailments. The present study was aimed to investigate and compare the phytoconstitutents present in both the flowers using TLC and HP TLC methods. The normal and double flo wers of P. granatum were extracted with chloroform and alcohol separately using soxhlet apparatus. The TLC and HPTLC finger print profile of the extracts with marker compounds were studied using HPTLC Instrument, CAMAG (Switzerland). The TLC and HPTLC profile of chloroform and alcohol extract of normal and double flowers of P. granatum were exploited. The compound gallic acid and ethyl gallate, chromatographed together served as marker compound. The results revealed th e similar and different phytoconstituents of both the flowers. The TLC and HPTLC finger print chromatograms not only demonstrates the similarities and differences bet ween the flowers but also sho ws the presence of tested marker compounds gallic acid and ethyl gallate in alcohol extracts of both the flower types apart from acting as a quality control tool.
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Sridhar, Nimmakayala, Suguna Lakshmi Duggirala, and Goverdhan Puchchakayala. "Analyzing the phytochemical composition of Justicia neesii Ramam." Journal of Phytopharmacology 3, no. 5 (2014): 348–52. http://dx.doi.org/10.31254/phyto.2014.3508.
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Phytochemical profile is an important aspect as it will give an over view of possible pharmacological properties of the plant. Justicia neesii is a plant belongs to Acanthaceae family, on which no significant phytochemical and pharmacological was done. The objective of the present study is to elucidate the phytochemical profile and analysis of antioxidant properties by TLC method. The phytochemical analysis was done for screening the maximum number of phytochemicals using standard methods. The TLC plates were developed with a solvent system containing methanol: chloroform: hexane at a ratio of 7:2:1. Ascorbic acid was used as positive control and a blank TLC plate was used as negative control in the experiment. The diluted DPPH in methanol was sprayed on the developed plates and observed under UV light. The preliminary phytochemical analysis shows the presence of flavonoids, glycosides, lactones, lignins, phenols, phytosterols, quinins, reducing sugars, saponins and terpinoids. The TLC analysis has shown the higher intensity of yellow color for the test spots which indicating the higher antioxidant potential of plant extract compared to standard ascorbic acid after treatment with DPPH solution. The plant is having good antioxidant potential. The plant was also composed of many significant phytochemicals.
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Rahman, Aziz ur, Sualeha Khan, Qazi Zaid Ahmad, and Huda Nafees. "Phytochemical Fingerprinting of a pharmacopoeial Unani formulation Habb e Asgand by TLC and HPLC for Standardization." Plant Science Archives 9, no. 4 (2024): 9–4. https://doi.org/10.51470/psa.2024.9.4.40.
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Habb-e- Asgandh a pharmacopoeial Unani preparation used for treating joints pain was formulated and then extracted in absolute ethanol and with the 50% alcohol. After drying these extracts, HPLC & TLC fingerprints were developed. TLC studies of Ethanolic and Hydroalcoholic extract of the formulation were carried out using two mobile phases (1) Toluene, Chloroform, Acetone and Acetic acid (70:15:15+2 drops) and (2) Benzene and Ethyl acetate (90:10). Thereafter, the TLC plates were observed in daylight, UV long and short wave length, and also treated with Iodine vapours and Anisaldehyde spray. Different numbers of spots were observed and Rf values alongwith colour were documented. HPLC of Ethanolic and Hydroalcoholic extract of Habb-e- Asgnadh was also carried out at 254 nm wavelength and the chromatogram was obtained. The mobile phase and flow rate was HPLC grade methanol and 1ml/min respectively. The HPLC profile of the Ethanolic extract of the test formulation gives 21 peaks and of the Hydroalcoholic extract of the formulation depicted total 6 peaks The TLC and HPLC profile of the test formulation can be used for standardization and quality control for future batches of Habb-e- Asgnadh.
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Solanke, Sachin N., Abhay S. Pohekar, and Jayshree A. Pohekar. "Clinical and laboratory profile of dengue fever: a retrospective study." International Journal of Advances in Medicine 6, no. 4 (2019): 1254. http://dx.doi.org/10.18203/2349-3933.ijam20193280.
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Background: The global incidence of dengue has grown dramatically in recent decade. Half of world’s population is now at risk. India represents significantly a larger burden, accounting for nearly 34% of the global burden of dengue infection. Dengue infection needs to be addressed as a single disease with different clinical presentations ranging from asymptomatic conditions to severe clinical courses that may lead to high morbidity and mortality.Method: This was retrospective observational study carried out during period of July 2017 to April 2018, to study clinical profile and laboratory parameters in dengue fever patients. Confirmed dengue cases having NS1 positive or IgM positive or having both NS1 and IgM positive or dengue ELISA reactive, having minimum one CBC reports done and not having other confounding factor such as co-infection, bone marrow diseases etc. that may altered clinical and laboratory results are included in study. Statistical analysis was done by SPSS software version 18.0.Results: Out of 48 confirmed dengue cases maximum patients 58.33% was from young age group (21 to 40 years) with M:F ratio was 2.43:1. Fever was found in 100% patients, in order of frequency followed by headache, bodyache, abdominal pain, weakness, retro-orbital pain, anorexia, dry cough, back pain, nausea, diarrhoea, vomiting, rash, joint pain, itching and malena.NS1was positive in 41.67% cases, dengue ELISA in 31.25%, IgM was positive in 20.83% cases, and both NS1 and IgM positive were in 4.17% cases. TLC count was low 35.42%, high in 12.50% of cases and remaining had normal TLC count. Platelet count was ranged between normal platelet counts to thrombocytopenia. One case had platelet count less than 20000. Out of 48 patients, 2 (4.17%) had malena.Conclusion: In this study, fever was found in all patients, and headache, body ache and weakness were common symptoms, but significant number of patients also had gastroentstinal and respiratory symptoms like abdominal pain, nausea, diarrhea, vomiting and dry cough. TLC count ranging from normal TLC, leukopenia to leucocytosis. Large number of patients had low platelet count that shows dengue fever had varied clinical presentation.
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Yenni Pintauli Pasaribu, Yorinda Buyang, Novike Bela Sumanik, La Hisa, and Johanis Wairata. "Antioxidant properties of Carallia brachiata stem bark and leaf." Technium: Romanian Journal of Applied Sciences and Technology 16 (October 29, 2023): 486–90. http://dx.doi.org/10.47577/technium.v16i.10035.
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Carallia brachiata (Lour.) Merr. (Rhizophoraceae) had been used to treat various human disoders. This study reported antioxidant activity of C. brachiata of stem bark and leaf in various solvents. Each sample was macerated in various solvent separately. By using DPPH, ABTS, and FRAP assays, antioxidant activity was determined. The TLC method was created using dichloromethane and ethyl acetate mobile phase. The methanol extract of stem bark contained the highest TPC value (262.73 ± 1.58 mg GAE/ g extract), while the methanol extract of leaf contained the highest TFC value (45.46 ± 0.32 mg QE/ g extract). The total phenolic content of stem bark extract was higher than that of leaf extract, but the flavonoid content of the leaf extract was marginally higher. The methanol extract exhibited the highest antioxidant activity for DPPH (13.06±0.08 μg/mL), ABTS (7.19±0.08 μg/mL), and FRAP assay (155.19±0.34 μM Fe2+/g). In this research, stem bark has greater antioxidant activity than the leaf. In addition, the results of TLC profile were detailed. The TLC chromatogram indicates that C. brachiata stem bark contains more components than leaf. This study indicates that C. brachiata has a rich source of prospective antioxidants.
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Rafi, Mohamad, Eti Rohaeti, Ali Miftahudin, and Latifah K. Darusman. "DIFFERENTIATION OF Curcuma longa, Curcuma xanthorrhiza and Zingiber cassumunar BY THIN LAYER CHROMATOGRAPHY FINGERPRINT ANALYSIS." Indonesian Journal of Chemistry 11, no. 1 (2011): 71–74. http://dx.doi.org/10.22146/ijc.21423.
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Turmeric (Curcuma longa), java turmeric (Curcuma xanthorrhiza) and cassumunar ginger (Zingiber cassumunar) are widely used in traditional Indonesian medicine. These three herbs have relatively similar rhizomes colour so it is difficult to be differentiated especially if they are in powder form. A rapid and reliable method, thin layer chromatography (TLC) fingerprint, has been developed in order to identify, authenticate and differentiate these three herbs through fingerprint profile of chemical compounds. TLC fingerprints of the three herbs were obtained by visualization of separate zones with visible and UV (254 and 366 nm) light. The TLC fingerprint pattern is different each other and showed a specific marker zones respectively. Therefore, TLC fingerprint can be utilized for identification, authentication and differentiation method in quality control of the three herbs tested.
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Ahmad, Islamudin, Rissyelly Rissyelly, Agus Kurniawan, and Abdul Munim. "SCREENING OF EXTRACTION METHOD FOR ALKALOID ENRICHMENT OF PEPEROMIA PELLUCIDA (L.) KUNTH." Asian Journal of Pharmaceutical and Clinical Research 10, no. 7 (2017): 214. http://dx.doi.org/10.22159/ajpcr.2017.v10i7.18426.
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Objective: The aims of this study were to investigate the effect of extraction method modification of Peperomia pellucida (L.) Kunth herbs for alkaloid enrichment and thin layer chromatography (TLC) profile.Methods: The dried herbs were extracted with six different methods (including; method A, B, C, D, and E). The obtained extract solution was evaporated and was analyzed. Alkaloid content identified using Dragendorff spray reagent. The gravimetric method was used to determine of total alkaloid, and TLC profile was analyzed using a densitometer at a wavelength of 254 nm and 366 nm.Results: The results showed a difference from all modification of extraction method for alkaloid enrichment and their TLC profile. The use of eluent dichloromethane (DCM):methanol (95:5) was potential for isolation of the compound. From whole extraction methods, only one that tested positive for alkaloids was method A. Total alkaloids in DCM fraction amounted to 29.59 mg/g piperine.Conclusion: Based on the above results, method A can be applied for isolation of alkaloid compound from this herb.
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Abriyani, Ermi, Lia Fikayuniar, Syifa Fauziah, and Lulu Melinda. "SKRINING FITOKIMIA DAN PROFIL KLT DARI FRAKSI N-HEKSANA DAN ETIL ASETAT PADA KULIT Pithecellobium jiringa (Jack) Prain." Jurnal Buana Farma 2, no. 3 (2022): 8–13. http://dx.doi.org/10.36805/jbf.v2i3.545.
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Jengkol (Pithecellobium jiringa (Jack) Prain) is a plant that is very often used as a popular processed food ingredient. Based on previous research that the seeds and rind of jengkol have a hypoglycemic effect and reduce glucose levels, and other ingredients are alkaloids, saponins, quinone and flavonoids. This study aims to determine the content of secondary metabolite compounds and the TLC profile contained in the rind of jengkol at fractionation of n-hexane and ethyl acetate. The methods are screening phytochemical, column chromatography, and the TLC. The phytochemical test showed that the rind of jengkol contained terpenoid, flavonoid and saponin compounds. Fraction A was shown spot bluish-green with Rf value 0,64 by the TLC test. The result of the TLC test was indicated to- saponin compound which is characterized by the presence of foam. The result of ethyl acetate fractionation was shown as light blue on The TLC chromatogram with an Rf value of 0,47 which is characterized flavonoid test.
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Adewunmi, C. M., and A. F. Jaiyeoba. "Assessment of Therapeutic Lifestyle Changes (TLC) in Lowering High Blood Cholesterol Among Academic and Non-Academic Staff of University of Lagos." International Journal of Advanced Studies in Engineering and Scientific Inventions 5, no. 1 (2024): 93–113. https://doi.org/10.48028/iiprds/ijasesi.v5.i1.09.
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High Blood Cholesterol is prevalent among academic and non-academic staff of University of Lagos from previous data assessment. The purpose of this study was to assess the effect of Therapeutic Lifestyle Changes (TLC) in lowering High blood cholesterol and to investigate the knowledge and practice of Therapeutic Lifestyle Changes among the staff of University of Lagos. Two research methods were adopted in this study; Experimental research method and Descriptive research method using a total sample of one hundred and seventy-nine participants.162members of staff both academic and nonacademic participated in the descriptive survey study while 17 participants (male and female) randomly selected from among the academic and non- academic staff from five faculties of the university participated in the experimental study. The experimental group was divided into two groups (study and control) by randomization 9 participants comprised the study group while 8 participants comprised the control group. The blood samples were taken and the lipid profile was measured in the University health service laboratory by a qualified medical laboratory scientist using spectrophotometer optima 300 machine. The pre-test of the lipid profile of participants measured before the commencement of the TLC intervention of six weeks was compared with the posttest lipid result after the six weeks both among each group and between the two groups using paired sample T-test at a p-value of less 0.05 level of significance. The result obtained revealed that there was a significant improvement in the lipid profile of the study group who went through a six-week TLC intervention while there was no any improvement in the lipid profile of the control group who did not receive any TLC intervention. Hence it is ascertained in this study that Therapeutic lifestyle changes has a lowering effect on high blood cholesterol.
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Janna, Nur, Fitriana Fitriana, and Sitti Amirah. "Potential of Endophyctic Fungi Isolates IFAZ-6 from Bidara Root (Ziziphus mauritiana Lam.) as Antibacterials Againts Digestive Tract Infection." Journal Microbiology Science 4, no. 1 (2024): 22–30. http://dx.doi.org/10.56711/jms.v4i1.995.
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Bidara root (Ziziphus mauritiana Lam.) has activity as an antibacterial against digestive tract infections. This study aims to determine the antibacterial activity of isolate IFAZ-6 endophytic fungi of bidara root against bacteria that cause digestive tract infections using the TLC-Bioautography method. Purification was conducted on the active isolate IFAZ-6 to obtain a pure endophytic fungi isolate. Subsequently, macroscopic and microscopic observations were carried out. Then, fermentation was performed in MYB (Maltose Yeast Broth) medium for 21 days. Screening and evaporation were conducted to produce an extract. The obtained extract was subjected to TLC analysis using a chloroform: methanol eluent (7:1). The TLC-Bioautography test was conducted against Escherichia coli, Salmonella typhi, Shigella dysentriae, and Vibrio cholerae bacteria. The TLC identification results showed one spot with an Rf value of 0.80 for the IFAZ-6 isolate. The chromatogram profile obtained from the TLC-Bioautography method for the IFAZ-6 isolate demonstrated antibacterial activity against bacteria that cause digestive tract infections.
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Wulandari, Lestyo. "EVALUATION OF RE-USED HPTLC PLATE FOR QUALITATIVE AND QUANTITATIVE ANALYSIS." Indonesian Journal of Chemistry 6, no. 3 (2010): 338–40. http://dx.doi.org/10.22146/ijc.21743.
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The eficiency chromatography of HPTLC plate is better than conventional TLC plate but it more expensive. Due to the higher price of HPTLC plate compare to conventional TLC plate, the application of ";re-used"; HPTLC plate has been tested. After being used for analyzing samples, the plates were developed again with the mobile phase, then dried for 60 min before using for the next measurement. The qualitative chromatography profile showed that ";re-used"; HPTLC plate has less contaminant than new HPTLC plate and the RSD of the precision quantitative testing of the plates reused three times showed a value > 2%. Keywords: reused, HPTLC, TLC, quantitative, qualitative
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Salim, Mohammad Fajar Hadi, I. Made Arie Dharma Putra Nugraha, Farah Adilla, and Luh Pratiwi Diva Yanti. "Chromatography Profiles of Terpenoid Compounds in The Extract of Sambiloto (Andrographis paniculata) Herb from Various Solvents." Walisongo Journal of Chemistry 4, no. 2 (2021): 74–80. http://dx.doi.org/10.21580/wjc.v4i2.7783.
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Sambiloto (Andrographis paniculata) is widely used as a medicinal plant for Indonesians. The content of extracted bioactive compounds is affected by the polarity of the extraction solvent. This study aims to determine the effect of extraction solvent on the chromatographic profile of Sambiloto herb, especially terpenoid group using the TLC-Spectrophotodensitometry method. Sambiloto herb was extracted by sonication method using methanol, ethanol, ethyl acetate, and chloroform as the solvents. Chromatographic profiles were identified by stationary phase of TLC silica gel GF254 plate and a mobile phase of chloroform:methanol (10:1 v/v), observed visually, and scanned at 210 nm. The plate was sprayed with anisadehyd-sulfuric acid reagent and observed visually. The compounds that belong to terpenoid compounds, namely S2, S5, S8, S11, and S12, had a greater percentage of peak area than the total area so that they were categorized as major components. The extraction solvent for Sambiloto herb that provides the best chromatographic profiles was found in the solvent of methanol extraction.
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ISA, Hassanatu, Katsayal Umar Adam mni Adam, Abdurahman Ezzeldin Mukhtar Abdurahman, Maje Idris Mohammed Mohammed, and Abdulhamid Zakir Abdulhamid. "Pharmacognostic studies and thin layer chromatography profile of the aerial parts of Vernonia cinerealess. (Asteraceae)." Journal of Current Biomedical Research 3, no. 6, November-December (2023): 1336–63. http://dx.doi.org/10.54117/jcbr.v3i6.3.
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Vernonia cinerea(VC) Less is synonymously known as Cyanthillium cinereum and Vernonia abbreviate belongs to Asteraceae family. Different parts of the plant are extensively used in traditional medicine for treatment of various disease conditions. However, there was no scientific evaluation of the plant’s pharmacognosy and thin layer chromatography (TLC), which necessitate these studies, to bring out characters that will facilitate its proper identification, standardization and, nature of secondary metabolites in aerial parts of the plant. To carry out detailed pharmacognostic studies and TLC evaluation on the aerial parts of Vernonia cinerea. Aerial plant parts of V. cinerea were studied for their organoleptic, macroscopic, microscopic, physicochemical features and bitterness properties, their elemental and nutritional values were evaluated using standard procedures. In the TLC, reagents were used to evaluate presence of various bioactive constituents in the parts studied. Pharmacognostic studies of aerial parts of VC highlighted features that are diagnostic from microscopic characters and their dimensions, different ergastic substances in the chemomicrocopical study. The elemental analysis showed the presence of macro, micro and trace elements. Nutritionally, there are presence of macro molecules such as carbohydrates, protein and fats. All these will serve as criteria to differentiate it from other species in the same and other families. The TLC profile showed spots of different colour shades after spraying with general and specific reagents and, their Rf values determined. Detailed pharmacognostic studies on the aerial parts of VC showed characters which might be useful to provide information with regard to its identification, standardization and types of phytochemicals present.
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LE POGAM, Pierre, Aline PILLOT, Françoise LOHEZIC-LE DEVEHAT, et al. "Mass spectrometry as a versatile ancillary technique for the rapid in situ identification of lichen metabolites directly from TLC plates." Lichenologist 49, no. 5 (2017): 507–20. http://dx.doi.org/10.1017/s0024282917000433.
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AbstractThin-layer chromatography (TLC) still enjoys widespread popularity among lichenologists as one of the fastest and simplest analytical strategies, today remaining the primary method of assessing the secondary product content of lichens. The pitfalls associated with this approach are well known as TLC leads to characterizing compounds by comparison with standards rather than properly identifying them, which might lead to erroneous assignments, accounting for the long-held interest in hyphenating TLC with dedicated identification tools. As such, commercially available TLC/Mass Spectrometry (MS) interfaces can be easily connected to any brand of mass spectrometer without adjustments. The spots of interest are extracted from the TLC plate to retrieve mass spectrometric signals within one minute, thereby ensuring accurate identification of the chromatographed substances. The results of this hyphenated strategy for lichens are presented here by 1) describing the TLC migration and direct MS analysis of single lichen metabolites of various structural classes, 2) highlighting it through the chemical profiling of crude acetone extracts of a set of lichens of known chemical composition, and finally 3) applying it to a lichen of unknown profile, Usnea trachycarpa.
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Santre, Monika G., Maharudra J. Sanap, Nandkumar Z. Gaikwad, et al. "Effect of Moringa oleifera Leaf Powder (MOLP) Supplementation on Haematological Profile in Transition Osmanabadi Goats." International Journal of Biochemistry Research & Review 34, no. 4 (2025): 79–89. https://doi.org/10.9734/ijbcrr/2025/v34i41006.
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Aims: The present study aimed at investigating the effect of feeding Moringa oleifera leaf powder (MOLP) on different haematological parameters during pregnancy in Osmanabadi goats at COVAS, Udgir, dist. Latur (Maharashtra state). Study Design: A total of 16 pregnant goats were randomly divide into two groups, comprising 6 animals each. Control (concentrate mixture without MOLP) and 20% MOLP supplemented group (concentrate mixture + 20% MOLP) /animal/day. Place and Duration of Study: Experimental animals were selected and fed on standard ration and supplements with MOLP at Livestock Farm Complex, College of Veterinary and Animal Sciences, Udgir. Dept of Veterinary Biochemistry, from July- October, 2024. Methodology: The experiment started after confirmation of pregnancy (1 month) till 2 weeks post parturition. Haematological parameters (haemoglobin, PCV, TEC, TLC, DLC, MCV and MCH) were measured and recorded individually at beginning of the experiment, on 60th day and postpartum 7th day. Results: The results of study revealed that haemoglobin, PCV and TEC showed no significant difference in all experimental groups. The MOLP supplementation resulted into significant (P<0.05) improvement in TLC values on 60th day in contrast to the control group. The DLC (granulocytes, lymphocytes) values were found highly significant (P<0.05) in MOLP supplemented group on 60th day and postpartum 7th day, however, the monocytes values did not differ significantly among the experimental groups. The values of MCV showed no significance difference in all experimental groups. The MCH concentration showed no significance, but the values were higher than the normal range in all experimental groups. Conclusion: The significant increase in granulocytes and lymphocyte counts could be attributed to enhanced immune response during pregnancy and 7th day partum stage in animals. The significant increase in total leucocyte count during pregnancy might be associated with enhanced phagocytosis against infectious diseases. Feeding of MOLP did not affect blood profile in pregnant Osmanabadi goats.
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Narwanti, Iin, and Nanik Sulistyani. "TLC-Bioautography Profile of Ethyl Acetate Extract of 5 Bacteria Isolated from Ficus carica L Rhizosphere." International Journal of Public Health Science (IJPHS) 4, no. 2 (2015): 81. http://dx.doi.org/10.11591/ijphs.v4i2.4716.
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Research has been conducted on the 5 isolates of bacteria isolated from the rhizosphere of Ficus carica L as a producer of antibiotics. The previous study showed they have NRPS gene profiles that differ from each other. This study aims to determine the TLC Rf spots having inhibition activity against the growth of Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. The study was conducted using the 5 bacteria isolates, namely T19, T24, T25, T37 and T41. All isolates were fermented at room temperature for 14 days. Further the each broth culture was filtered and extracted using ethyl acetate. Components in the extract were separated by thin layer chromatography (TLC) with the mobile phase of chloroform-methanol (7: 3), followed by bioautography test against the S. aureus and E. coli to determine the chromatogram spots containing antibiotics. TLC results showed all isolates had different chromatogram profiles. The bioautography results showed that only isolate T25 can produce antibiotics against S. aureus. The antibiotic spot was at Rf 0.9 in the use of chloroform-methanol (7: 3) solvent system.
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Narwanti, Iin, and Nanik Sulistyani. "TLC-Bioautography Profile of Ethyl Acetate Extract of 5 Bacteria Isolated from Ficus carica L Rhizosphere." International Journal of Public Health Science (IJPHS) 4, no. 2 (2015): 81. http://dx.doi.org/10.11591/.v4i2.4716.
Full textAbstract:
Research has been conducted on the 5 isolates of bacteria isolated from the rhizosphere of Ficus carica L as a producer of antibiotics. The previous study showed they have NRPS gene profiles that differ from each other. This study aims to determine the TLC Rf spots having inhibition activity against the growth of Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. The study was conducted using the 5 bacteria isolates, namely T19, T24, T25, T37 and T41. All isolates were fermented at room temperature for 14 days. Further the each broth culture was filtered and extracted using ethyl acetate. Components in the extract were separated by thin layer chromatography (TLC) with the mobile phase of chloroform-methanol (7: 3), followed by bioautography test against the S. aureus and E. coli to determine the chromatogram spots containing antibiotics. TLC results showed all isolates had different chromatogram profiles. The bioautography results showed that only isolate T25 can produce antibiotics against S. aureus. The antibiotic spot was at Rf 0.9 in the use of chloroform-methanol (7: 3) solvent system.
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Thatipelli, Sujith, Achintya Kumar Mandal, and Shakila Ramachandran. "Chemical Standards and HPTLC Finger Print Profiles of a Siddha Polyherbal Formulation - Kadukkai Legiyam." International Journal of Ayurvedic Medicine 12, no. 4 (2021): 922–26. http://dx.doi.org/10.47552/ijam.v12i4.2188.
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To study physico-chemical, phytochemical and high performance thin layer chromatography of a Siddha drug “Kadukkai Legiyam” (KL). The prepared Kadukkai Legiyam (KL) was prepared as per the standard operating procedures mentioned in literature. Then the drug was subjected to physicochemical parameters, phytochemical screening, thin layer chromatographic photo documentation (TLC), high performance thin layer chromatographic (HPTLC) finger print profile of hexane, chloroform, ethanol and hydro alcohol (1:1) extracts. Different extracts of the drug showed distinct TLC and HPTLC finger print patterns which will be unique to this drug. This study giving information about physiochemical and phytochemical analysis and HPTLC fingerprint profile of different extracts, the integration spectrum which will useful in standardizing the raw drugs and future comparison studies.
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Khatak, Sunita. "TLC (Thin Layer Chromatography) to construct chemical fingerprints of Ashwagandha (W. Somnifera) – A Rejuvenating Herb." Research Journal of Biotechnology 20, no. 3 (2025): 181–90. https://doi.org/10.25303/203rjbt1810190.
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Withania somnifera has been used in Ayurvedic medicine for the treatment of depression and inflammation and as an aphrodisiac. It contains many phytochemicals such as Withaferin A, Withanine, Withanolides and Anahygrine. Withaferin ‘A’ being the most active compound was estimated in herbal extracts and polyherbal formulation using TLC in number of studies from Withania somnifera. Dried leaves and roots from each of the 30 genotypes were extracted for phytochemicals using methanolic extracts. Chromatograms were developed using solvent system consisting of chloroform: methanol (90:10) for methanolic extracts and a mobile phase consisting of toluene: ethyl acetate: formic acid (75:18:7) on precoated TLC aluminum plates Si60F254 for methanolic leaf extracts from each of the 30 genotypes. Based on the TLC profile of phytochemicals obtained from leaves and roots, all the 30 genotypes of ashwagandha were grouped together in five major clusters irrespective of their geographical conditions. The genotypes did not reveal a clear cut grouping or clustering of genotypes of particular locations but were found to be interspersed with each other. The first cluster revealed 15 genotypes from different locations namely, Udaipur (Raj.), Mandsaur (M.P.) and that of Haryana showing relation among them. The TLC profile of root phytochemicals resulted in grouping of various genotypes in five clusters and here also the genotypes did not form distinct location specific clusters but were interspersed with each other similar to earlier reports.
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Praptiwi, F. I. Windadri, D. Sulistiarini, L. Hafid, D. Sahroni, and D. Surya. "GC-MS profile, total phenolic, and DPPH radical scavenging activity of Saurauia minahassae stembark ethyl acetate extract." IOP Conference Series: Earth and Environmental Science 1312, no. 1 (2024): 012033. http://dx.doi.org/10.1088/1755-1315/1312/1/012033.
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Abstract Several species of the genus Saurauia have been traditionally used as remedies. However, Saurauria minahassae, as an endemic plant, is still understudied. This study aims to reveal the total phenolics, GC-MS profile, and bioactivity as DPPH free radical scavengers of ethyl acetate extract of S.minahassae collected from Banggai Island, Indonesia. Determination of Total Phenolic Content (TPC) was conducted using the colorimetry method by spectrophotometer. Thin layer chromatography (TLC) and TLC-bioautography performed analysis of secondary metabolites and qualitative DPPH free radical scavenging activity, respectively. GC-MS analysis was conducted to identify the chemical compounds of the extract. The extract’s IC50 value and Antioxidant activity index (AAI) were performed by serial microdilution method on the 96-microwell plate. The results showed that TPC was 432.33 mg GAE/g extract. TLC analysis revealed several metabolites, and TLC-bioautography also revealed several compounds active as DPPH free radical scavengers. The ethyl acetate extract of S. minahassae is categorized as a very strong DPPH free radical scavenger or potent antioxidant with an IC50 value of 3.29 ug/ml and AAI of 9.32. GC-MS profile of the ethyl acetate extract of S.minahassae stembark contained several compounds with antioxidant activity. The potential antioxidant activity of S. minahassae stembark extract was contributed by phenolic content and several compounds with antioxidant activity in the extract. This extract could be a good source of natural antioxidants, and S. minahassae sustainability as an endemic plant should be maintained.
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BHARTI, ANU, JYOTI BABU DHAR, and TEJ KRISHAN BHAT. "The content, profile and biological activity of tannins in some tanniniferous plants." Journal of Medicinal and Aromatic Plant Sciences 37, no. 3 (2015): 46–51. http://dx.doi.org/10.62029/jmaps.v37i3.bharti.
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The present study was carried out on the quantification, chromatographic profiling and biological activity assessments of selected tanniniferous forages viz. leaves of oak/ban (Quercus leucotrichophora), robinia (Robinia pseudoacacia), khirk (Celtisaustralis), kachnar (Bauhinia variegata), siris (Albizia lebbeck), pakar (Ficus infectoria), tremal (Ficus roxburghii) and buince (Salix alba), and leaves and fruits of bhera (Terminalia bellerica) and harad (Terminalia chebula). The total tannin content was found high in harad and bhera fruits & leaves. Kachnar, robinia and pakar leaves were rich source of condensed tannins. However, hydrolysable tannins were the predominant component of the total tannin phenols of oak, harad and bhera leaves. The extraction of tannins was followed by TLC fingerprinting. TLC fingerprint analysis of selected plants showed remarkable difference in the overall complexity and the polarity of the tannins. Prospections were also carried out on the protein precipitating capacity and free radical scavenging activity of these tannins. The antioxidant activity of the plant samples was done using 1,1-Diphenyl-2-picryl-hydrazyl spray. The protein precipitation capacity and antioxidant activity – the major indicators of biological activity of tannins, suggested that a fine balance has to be struck between the beneficial and deleterious effects of these tannins by maintaining a control over their concentration vis-à-vis their intake by the animals.
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Syarifah, Anisa Lailatusy, Rurini Retnowati, and Hermin Sulistyarti. "Characterization of the Curcuminoids Fingerprint Profile in Curcuma and Zingiber Genera by TLC – Digital Image Analysis." Journal of Pure and Applied Chemistry Research 8, no. 2 (2019): 147–61. http://dx.doi.org/10.21776/ub.jpacr.2019.008.02.462.
Full textAbstract:
Curcuma longa, C. xanthorrhiza, C. heyneana, and Zingiber cassumunar contain high curcuminoid and have relatively similar yellow color. Therefore, they are potentially adulterated and difficult to differentiate in the form of powder. Hence, it is necessary to characterize the fingerprints compound profile by a simple and rapid method. This research aims to determine fingerprint compound profile of curcuminoid using Thin Layer Chromatography (TLC) and digital image analysis. The result of the research identified that the fingerprint compound profile of curcuminoid on the four rhizomes was obtained by TLC method using silica gel 60 GF254 as the stationary phase, chloroform: dichloromethane: methanol (13:6:1) as the mobile phase, and observation under UV 254 nm light and citroborate reagent. Thereafter, the digital image analysis was carried out using Image J software according to the gray value and % of RGB (red-green-blue) value. Based on gray value and % of RGB, both Curcuma and Zingiber genera were differentiated through curcumin compound (Rf 0.63), demethoxycurcumin (Rf 0.34), bisdemethoxycurcumin (Rf 0.21). The profile of fingerprint compound on Curcuma longa, C. xanthorrhiza, C. heyneana, and Zingiber cassumunar was differentiated through Rf 0.26; Rf 0.17; and Rf 0.10.
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Syarifuddin, Alfian. "PROFIL KLT-BIOAUTOGRAFI DAN DENSITOMETRI SENYAWA TERAKTIF (ISOLAT KP13) DARI RIZOSFER KAYU PUTIH." Jurnal Farmasi Sains dan Praktis 5, no. 1 (2019): 21–25. http://dx.doi.org/10.31603/pharmacy.v5i1.2291.
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The rate of infection, especially in Indonesia is still high. Treatment of infections by using antibiotics is constrained by cases of resistance to antibiotics so that it is necessary to explore microorganisms that can produce antibiotics. Antibiotics produced by KP13 bacterial isolates have the potential as antibiotics against Escherichia coli. The purpose of this study was to determine the TLC-Bioautography profile and Densitometry analysis of the most active fraction of KP13 bacterial isolates which have the potential as antibiotics against Escherichia coli bacteria. TLC using the mobile phase Chloroform: Ethylacetate: Methanol (4: 1: 0.5). Active compounds were analyzed using GC-MS. The results showed active spots during TLC-Biaoautography with a Rf value of 0.78. Compounds that are thought to be responsible for antibiotic activity detected by GC-MS at a Rf value of 0.78 have similarities with the compounds of cycloheptatriene and tetrahydrophyran..
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Pandey, Swati, Divyaansh Singh, Jagat Pal Yadav, and Amita Verma. "Medicinal Potential of Curcuma caesia Roxb.: Phytochemical Composition and TLC Profile of Hydroalcoholic Rhizome Extract." Journal of Drug Delivery and Therapeutics 15, no. 6 (2025): 41–47. https://doi.org/10.22270/jddt.v15i6.7181.
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Curcuma caesia (black turmeric) is a medicinal plant traditionally used in Southeast Asia for the treatment of various ailments, including inflammation and cancer. This study aimed to evaluate the phytochemical profile and chromatographic characteristics of its hydroalcoholic rhizome extract to explore its anticancer potential. The rhizome extract was subjected to preliminary phytochemical screening to identify major classes of bioactive compounds. Thin Layer Chromatography (TLC) was performed using six different solvent systems of varying polarity to achieve comprehensive phytochemical profiling. Chromatograms were visualized under UV light (365 nm), and Rf values were calculated to assess compound diversity and migration behavior. Phytochemical screening revealed the presence of flavonoids, phenolics, alkaloids, terpenoids, saponins, and glycosides. TLC profiling showed distinct separation patterns across all solvent systems, with the most effective resolution observed in Methanol:Ethyl Acetate (2:8) and Toluene:Ethyl Acetate (2:8) systems. A total of 69 phytoconstituent bands were detected across all chromatograms, with Rf values ranging from 0.08 to 0.94, indicating high chemical diversity. Bright fluorescent spots under UV light suggested the presence of conjugated aromatic compounds typical of phenolics and flavonoids. The study highlights the rich phytochemical composition and chromatographic complexity of Curcuma caesia rhizome extract, supporting its potential as a natural source of anticancer agents. The presence of biologically active compounds, especially flavonoids and terpenoids. Keywords: Curcuma caesia, phytochemical screening, TLC profile, rhizome extract.
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Hetal, B. Gavit 1. *. Dr. N.M. Patel 2. "QUANTITATIVE PHYTOCHEMICAL ANALYSIS AND HPTLC FINGERPRINTING OF AMARANTHUS BLITUM L. LEAVES FROM SOUTH GUJARAT." Journal of Pharma Research 8, no. 5 (2019): 288–93. https://doi.org/10.5281/zenodo.3236665.
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<strong><em>ABSTRACT</em></strong> <strong><em>T</em></strong><em>he present investigation was aimed to carry out for the quantification of phytochemicals and HPTLC fingerprinting profile of methanolic extract of Amaranthus blitum L. leaves<strong>. </strong>The pharmacologically important phytochemicals such as alkaloids, flavonoids, saponins, terpenoids, lipid, crude fibre, chlorophyll and carotenoids were determined as per the standard procedures. The leaves were rich in flavonoids followed by saponins and alkaloids. The TLC of flavonoids also showed more number of compounds. The methanolic extract, upon HPTLC study revealed 15 numbers of different constituents with R<sub>f</sub> values ranging from 0.08 to 0.81 in fingerprinting profile. The results of this study will aid to identity and check quality for authenticity of Amaranthus blitum L. leaves.</em> <strong><em>KEYWORDS:</em></strong><em> Amaranthus blitum, Phytochemical, TLC, HPTLC, Methanolic extract.</em>
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Bhattacharjee, Indranil, Anupam Ghosh, Nandita Chowdhury, Soroj Kumar Chatterjee, Goutam Chandra, and Subrata Laskar. "n-Alkane Profile of Argemone mexicana Leaves." Zeitschrift für Naturforschung C 65, no. 9-10 (2010): 533–36. http://dx.doi.org/10.1515/znc-2010-9-1001.
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An n-hexane extract of fresh, mature leaves of Argemone mexicana (Papaveraceae), containing thin-layer epicuticular waxes, has been analysed for the first time by TLC, IR and GLC using standard hydrocarbons. Seventeen long-chain alkanes (n-C18 to n-C34) were identified and quantified. Nonacosane (n-C29) was established as the n-alkane with the highest amount, whilst octadecane (n-C18) was the least abundant component of the extracted wax fraction. The carbon preference index (CPI) calculated for the hydrocarbon sample with the chain lengths between C18 and C34 was 1.2469, showing an odd to even carbon number predominance.
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Susanti, Ni Made Pitri, Luh Putu Mirah Kusuma Dewi, Harlina Setiawati Manurung, and I. Made Agus Gelgel Wirasuta. "IDENTIFIKASI SENYAWA GOLONGAN FENOL DARI EKSTRAK ETANOL DAUN SIRIH HIJAU (PIPER BETLE LINN.) DENGAN METODE KLT-SPEKTROFOTODENSITOMETRI." Metamorfosa: Journal of Biological Sciences 4, no. 1 (2017): 108. http://dx.doi.org/10.24843/metamorfosa.2017.v04.i01.p16.
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Phenol compound in Piper betle leaves has several pharmacology activities such as antibacteria, antifungi and antioxidant. The pharmacology activities of a herbal drug are influenced by the phytochemistry content, so in order to do a quality determination that provides phytochemistry profile and consistent pharmacology activities, a standardization is required. Fingerprint is the main standard to perform quality control for herbal drug. TLC-spectrophotodensitometry was used as the method in order to provide fingerprint profile of phenol compound. In this experiment, Piper betle leaves samples were extracted by reflux method using ethanol 96% as the solvent. Identification of phenol compound was done using TLC-spectrophotodensitometry with Silica gel 60 F254 as the stationary phase, toluena: ethyl acetate (93:7 v/v) as the mobile phase, FeCl3 and Folin-Ciocalteau as the reagent. The fingerprint profile of phenol compund was shown in Rf value 0,19; 0,42; and 0,62. Positive results of phenol compound are black spot on FeCl3 colour test and dark blue spot on Folin-Ciocalteau colour test. Maximum wavelength of phenol compound was 283 nm.
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Owolabi, Tunde, Dominion Osaretin, and Blessing Eyinayan. "Bioactive composition and TLC profile data on Pax Herbal Malatreat Tea." Drug Analytical Research 6, no. 1 (2022): 35–39. http://dx.doi.org/10.22456/2527-2616.125038.
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The biological/pharmacological activities of any herbal drug are determined by the bioactive components contained in the plant(s) used in its formulation, these substances are greatly affected by the quality control system adopted during and after production processes and handling of such product. Thin Layer Chromatography is one of such quality control parameters that demonstrate uniqueness and uniformity between various substances, thus serving as an identity for such products. Pax Herbal Malatreat tea is a polyherbal drug used in the management and treatment of malaria symptoms. This study evaluated the different phytoconstituents present and developed fingerprint profiles (TLC) for the drug (Pax Herbal Malatreat Tea) to serve as a quality control check during the production consistency and market uniqueness. Qualitative phytochemical and chromatographic analyses were carried out using standard methods. The qualitative test revealed the presence of Tannin, Flavonoid, Saponin, Steroids, Reducing sugar, and Terpenoids, and the finger-print chromatograms after development with chloroform-ethyl acetate (9.5:05) showed five distinct components at 365 nm and four spots when sprayed with 20% methanolic sulphuric acid and viewed under visible light. From this present report, an identity card has been developed for the drug (Pax Herbal Malatreat Tea) via bioactive composition and TLC profiles which can be used in accessing the quality and consistency of the product.
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Mawardi, Rizky H., Nanik Sulistyani, Nurkhasanah Nurkhasanah, and Ricke Desyratnaputri. "ANTIBACTERIAL ACTIVITY AND TLC-BIOAUTOGRAPHY ANALYSIS OF THE ACTIVE FRACTIONS OF Muntingia calabura L. LEAVES AGAINST Staphylococcus aureus." Journal of Pharmaceutical Sciences and Community 17, no. 2 (2020): 69–75. http://dx.doi.org/10.24071/jpsc.002362.
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This study aims to determine the antibacterial activity and TLC-bioautography profile of the active fractions of Muntingia calabura L. leaves against Staphylococcus aureus. Muntingia calabura L. leaves were macerated with ethanol 96% then fractionated with n-hexane, ethyl acetate, and methanol solvent, respectively. The antibacterial activity was tested by the Kirby-Bauer method to determine the most active fraction and the lowest concentration that inhibited the growth of Staphylococcus aureus. TLC-bioautography was tested using chloroform: ethyl acetate (2:8) as the mobile phase and silica gel F254 as the stationary phase. Antibacterial activity test of n-hexane and ethyl acetate fractions at a concentration of 10% w/v showed activities with inhibition zone diameter of 0.33±0.288 and 9.66±5.77 mm, respectively. At the same time, the methanol fraction showed no activity. The lowest concentration of ethyl acetate fraction which still showed the inhibition zone was 0.312% w/v. The TLC-bioautography profile showed active spots with an Rf value of 0.82 and had an inhibitory zone diameter of 4.013±0.864 mm. It can be concluded that the ethyl acetate fraction was the most active fraction that inhibited the growth of Staphylococcus aureus and had one active spot on the bioautography test.
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Wahyuningsih, Etik, and Isnaeni. "Identification of Paracetamol and Caffeine in Jamu Powders Simultaneously Using TLC-Densitometry." Berkala Ilmiah Kimia Farmasi 10, no. 1 (2023): 18–22. http://dx.doi.org/10.20473/bikfar.v10i1.44242.
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Paracetamol and caffeine are chemical compounds that are suspected to be illegally added to the traditional herbs claimed as rheumatics drugs. Identification of paracetamol and caffeine has been done on five samples of jamu powder obtained from the Depot Jamu in Surabaya. This study aimed to simultaneously identify paracetamol and caffeine commonly found in traditional medicine one of which is jamu powder using thin-layer chromatography densitometry (TLC-Densitometry). Evaluate the presence of paracetamol and caffeine in the product of jamu was performed by thin layer chromatography with silica gel GF254 and chloroform-ethyl acetate (1:1) as the stationary and mobile phase respectively. The spots on the TLC plate were detected using a UV at 254 nm and the areas were measured by a Camag TLC scanner. The TLC profile demonstrated a good separation of paracetamol, caffein, and others substances that containing in the products. The retardation factor (Rf) of paracetamol and caffeine were 0,42 and 0,26 with a detection limit of 0,0125 µg/spot and 0,05 µg/spot respectively. The simultaneous identification of caffeine and paracetamol by using thin-layer chromatography densitometry revealed that none of the five samples were detected to contain paracetamol and caffeine. Keywords: Simultaneously, Identification, Paracetamol, Caffeine, Jamu Powder, TLC- Densitometry
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Hartanti, Dwi, and Jirapat Theeravit. "Quality Control Of Crude Drugs And Capsules Of Turmeric (Curcuma longa L.)." PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) 15, no. 2 (2018): 257. http://dx.doi.org/10.30595/pharmacy.v15i2.2811.
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Turmeric (Curcuma longa L.) is a medicinal plant commonly used in Thai Traditional Medicine (TTM), both in single use and formula. It has been known for its antioxidant activity and applied for management of many diseases. In this article, we reported some aspects of quality control of crude drugs and capsules of turmeric according to the standards set in Thai Herbal Pharmacopeia (THP). The results of our evaluations demonstrated that turmeric crude drugs met the criteria set in THP for microscopical identification, foreign matter (1.31%), loss on drying (6.89±0.174%), ethanol-soluble extractive (13.56%), water-soluble extractive (15.17%), and the profile of Thin Layer Chromatography (TLC) chromatogram. However, its volatile oil content (5.95%) was below the minimum value set in THP. The turmeric capsules met the criteria set in THP for loss on drying (8.64±0.093%), ethanol-soluble extractive (18.07%), water-soluble extractive (14.95%), and profile of TLC chromatogram.
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Allu, Rajesh, Padmini D, Indhumathi M, et al. "Antimicrobial activity and Quality Control Parameters of Talicati Vatakam - A Classical Siddha Drug." International Journal of Ayurvedic Medicine 13, no. 2 (2022): 463–68. http://dx.doi.org/10.47552/ijam.v13i2.2600.
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Tāḷicāti vaṭakam (TSV), a polyherbal siddha drug was chosen, it was screened for antimicrobial study and also subjected to standardization parameters. The ingredients were procured, authenticated and prepared the drug as per standard operating procedure. The ethanolic extract of TSV was screened for nine bacteria and two fungi. Then the drug was investigated for the phytochemical profile, physicochemical parameters, thin layer chromatographic photo documentation (TLC), high-performance thin-layer chromatography (HPTLC) finger print profile. Antimicrobial assay revealed inhibitory activity against all test pathogens. TLC under UV showed 8 bands at short wavelength, 13 bands at long wavelength; 8 bands showed post derivatization with vanillin Sulphuric acid reagent. The present investigation concluded that the siddha herbal preparation of TSV have great potential on antimicrobial against pathogens. This siddha formulation can be used to prevent the bacterial and fungal infections and the standards could be used for quality control of the drug.
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Joshi, Rhythm, Zehva Khan, Aakriti Garg, et al. "Short-Term Impact of Hematopoietic Stem Cell Transplantation in Leukemia Patients on Bone Bio Markers, Electrolytes and Blood Profile." OBM Transplantation 07, no. 02 (2023): 1–14. http://dx.doi.org/10.21926/obm.transplant.2302183.
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Hematopoietic stem cell transplantation (HSCT) or Bone Marrow Transplantation (BMT) has significantly improved the survival rates of patients suffering from hematological malignancies. However, the cure can only be achieved at the price of morbidity and long-term complications such as bone diseases leading to fractures and osteopenia. Several studies have reported the impact of organ transplants on bone metabolism, electrolytes and blood profile. This prospective observational clinical study aims at elucidating the effects of HSCT on bone metabolism, electrolytes and blood profile in leukemia patients. Sixty patients were included in this study. The current study aimed to evaluate the short-term (30 days) impact of HSCT on bone biomarkers [osteoprotegerin (OPG) and alkaline phosphatase], electrolytes (calcium, sodium, potassium, and magnesium), and blood profile [hemoglobin, absolute neutrophil count (ANC), platelet, and total leucocyte count (TLC)] in leukemia patients undergoing HSCT from their record files before HSCT (TP1) and after 30 days of HSCT (TP2). Further, the correlation among various parameters at TP2 was assessed using Spearman correlation analysis. At TP2, the level of OPG, alkaline phosphatase, calcium, magnesium and TLC increased significantly compared to TP1. However, no significant change was observed in other parameters at TP2 compared to TP1. A statistically significant positive correlation of TLC with hemoglobin and sodium; and magnesium with alkaline phosphatase was observed. Furthermore, a significant negative correlation between hemoglobin and calcium was observed. Even though there was a statistically significant increase in the level of OPG, alkaline phosphatase, calcium, magnesium and hemoglobin after 30 days of HSCT, the level of bone biomarkers, electrolytes and blood profile were in the normal physiological range. However, additional studies are required to get a detailed understanding of the changes in parameters after HSCT.
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Olanrewaju, Timothy O., Patricia O. Odumosu, and Kenneth O. Eyong. "Anti-trypanosomal evaluation of Ximenia americana root bark and chromatographic-mass spectrometric profile." GSC Biological and Pharmaceutical Sciences 7, no. 2 (2019): 108–17. https://doi.org/10.5281/zenodo.4294626.
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Medicinal plants are the richest bio-resource of drugs for traditional systems of medicine, modern medicines, food supplements, pharmaceutical intermediates and chemical entities for synthetic drugs. Human African Trypanosomiasis (HAT) is a challenging and deadly disease due to its complex epidemiology and clinical presentations. This study was conducted to investigate anti-trypanosomal action of <em>Ximenia americana</em> root bark on <em>Trypanosoma brucei brucei</em> using various solvent extracts and to develop thin layer (TLC-MS) and liquid chromatography-mass spectrometric (LC-MS) profiles of the plant. Soxhlet extraction was used to obtain acetone, 70% ethanol total extracts in addition to n-hexane, dichloromethane, ethyl acetate and methanol fractions by sequential extraction. The inhibitory activity of the various extracts was compared by testing against <em>T. b. brucei</em> using isometamidium chloride as standard drug. The most active extract was separated by solid-phase extraction (C18 stationary phase) to obtain fractions which were profiled by TLC-MS (+ESI) and LC-MS. It was observed that anti-trypanosomal activity of acetone (16.83% yield) and 70% ethanol (18.23% yield) were comparable. However, methanol extract exhibited the highest activity with 99.18%, 97.5% and 87.50% inhibition at 3 h incubation (room temperature) using 1000 µg, 500 µg and 250 µg concentrations respectively. The activities at 1000 µg for methanol extract and isometamidium chloride were comparable with 95% CI [-1.10, 1.77]. TLC-MS and LC-MS analyses suggested gallic acid, 2,3,4,5-tetrahydroxybenzoic acid, 2ʹ,5-dimethoxyflavone, quercetin, dihydroquercetin and sesquiterpene when compared with literature database. This study presents data that could be useful in standardisation and preparation of alternative medicine in the treatment of African trypanosomiasis.
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P., Sravan Kumar 1. *. A. Santhosh 2. K. Manasa M. Swapna 3. M. Sreevani 3. M. Bhavusingh 3. M. Mamatha 3. "IN-VITRO ANTIHELMINTHIC ACTIVITY, PHYTOCHEMICAL SCREENING AND TLC STUDIES OF ETHANOL-WATER EXTRACTION ON IPOMEA CARNEA FLOWER USING IN - STATE FESTIVAL OF TELANGANA (BATHUKAMMA)." Journal of Pharma Research 7, no. 4 (2018): 50–55. https://doi.org/10.5281/zenodo.1228663.
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<strong><em>ABSTRACT</em></strong> <strong><em>I</em></strong><em>ndia Being a Rich and Varied Flora of Medicinal Plants. It’s used in Traditional Festivals of India. The Present Study deals with the In-Vitro Antihelminthic Activity, Phytochemical Screening and TLC Studies of Ethanol:Water (80:20) Extraction on Ipomea Carnea Flower using in </em><em>- State Festival of Telangana (Bathukamma).</em><em> Ethanol:Water (80:20) Extraction on Ipomea Carnea Flower was used evaluation of Phytochemical Screening Determination by some chemical tests and Thin Layer Chromatographic Study was carried out by using Various Solvent System of varying Polarity of Hexane, Ethyl Acetate, Acetone. Phytochemical Screening Reflects Presence of like Carbohydrates, Alkalides, Phenols, Tannins, Phytosterols, Glycosides, Flavonoids, Tannies Shows Methanol Solvents Extracts. Thin Layer Chromatographic Studies of the Ipomea Carnea Flower Parts Constituted Different Colored Phytochemical Compounds with different R<sub>f</sub> Values. Ethanol:Water (80:20) Extractions of Ipomea Carnea Flower Various Concentrations (25, 50, 100mg/ml) of all Extracts were Tested and Results were Expressed in Terms of time for Paralysis and time for Death of Worms. Piperazine Citrate (10 mg/ml) was used as a Reference Standard and Distilled Water as a Control Group.</em><em> Treatment with </em><em>Concluded that the Ethanol:Water (80:20) Extract of Ipomea Carnea Flowers showed Potent Anthelmintic Activity and was Equipotent to Standard Anthelmintic drug</em><em>. The </em><em>Potent Anthelmintic Activity</em><em> could be due to Presence of Glycosides, Flavonoids and Sterols. So, from the above Findings, it was Concluded that </em><em>Ethanol:Water (80:20) Extract of Ipomea Carnea Flowers </em><em>Posse’s Significant </em><em>Wormicide Activity</em><em> Property.</em> <strong><em>KEYWORDS:</em></strong><em> Ipomea Carnea, Anthelmintic, Phytochemicals, TLC Profile.</em>
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Ayobami Owolabi, Tunde, and Emmanuel Amodu. "Bioactive composition and TLC profile data on PAX herbal health tea and PAX herbal diatea." International Journal of Advanced Chemistry 10, no. 2 (2022): 46–49. http://dx.doi.org/10.14419/ijac.v10i2.32112.
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Background: The phytoconstituents of herbal drugs are largely influenced by the quality control system used during and post-production processes including the handling of such products. Thin Layer Chromatography is one of such quality control parameters that demonstrate uniqueness and uniformity between various substances, serving as an identity for such substances. Pax Herbal Health tea (PHT) and Pax Herbal Diatea (PDT) are polyherbal drugs, PHT is used as a tonic for general wellness, while, PDT is used in the management and treatment of diabetes. This study evaluated the different phytoconstituents present and developed Thin-layer chromatography (TLC) fingerprint profiles for PHT and PDT to serve as quality control checks during the production for consistency and market uniqueness after production.Material and Methods: Qualitative phytochemical and chromatographic analyses were carried out using standard methods.Results: The phyto-screening revealed the presence of Alkaloid, Flavonoid, Tanin, Terpenoids, Reducing sugar, Steroid, and Cardiac glycoside in PHT while, Saponin, Tanin, Steroids, Reducing sugar, Flavonoid, and Terpenoids were observed in PDT. The TLC finger-print chromatograms of PHT after development with n-Hexane:Ethyl acetate (3:2) showed four distinct components under ultraviolet light at 365 nm, and three spots when sprayed with 20% methanolic sulphuric under visible light, while, PDT developed in n-Hexane:ethyl acetate:methanol (2.5:2:0.5) revealed three fluorescent components at 365 nm and four components after sulphuric acid treatment.Conclusion: From this present study, identity cards have been designed for PHT and PDT through bioactive composition and TLC profiles which can be used in accessing the quality and consistency of the herbal drugs. Â
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Hasan, Akhmad Endang Zainal, Dimas Andrianto, Husnawati, et al. "ISOLATION AND ANTI-YEAST ACTIVITY OF SECONDARY METABOLITES OF SOURSOP LEAF ENDOPHYTIC FUNGI." Indonesian Journal of Applied Research (IJAR) 3, no. 1 (2022): 56–66. http://dx.doi.org/10.30997/ijar.v3i1.184.
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This study aimed to isolate the endophytic fungi of soursop leaves that have the potential as anti-yeast. Soursop leaves were collected from 3 locations in West Java, Indonesia. The secondary metabolites produced by the endophytic fungi were extracted with ethyl acetate and tested for anti-yeast activity against Saccharomyces cerevisiae and Candida tropicalis. Profile of extracts contents characterization was carried out by using thin-layer chromatography (TLC). Twelve monoculture soursop leaves endophytic fungi isolates from Sukabumi (3 isolates), Cianjur (3 isolates), and Garut (6 isolates). It showed different abilities to inhibit C. tropicalis and S. cereviseae. The best extract is isolat from Cianjur (Sir-C1) and Sukabumi (Sir-S1). The stain patterns seen between the nine endophytic fungi extracts were similar. This indicates that the compounds in them may also be the same. But the extracts have a different absorbance for anti-yeast activities. This indicates that the total metabolites of each isolate are different. TLC results showed that ethyl acetate extraction produced almost the same metabolite components from nine endophytic fungi extracts, and all extracts had an anti-yeast activity with various absorbance. There are differences in the ability of 12 soursop leaf endophytic fungi to inhibit yeast model C. tropicalis and S. cerevisiae. Some extracts have similar TLC profiles, but their anti-yeast activity is not similar
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Musial, Beata A., André J. Sommer, and Neil D. Danielson. "Microchannel Thin Layer Chromatography with in situ Plate Scanning Micro-DRIFTS Detection Using Plain and Polybutadiene Modified Zirconia Stationary Phases." Applied Spectroscopy 56, no. 8 (2002): 1059–66. http://dx.doi.org/10.1366/000370202321274971.
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Thin layer chromatography (TLC) of various dyes is compared in microchannels packed with either bare zirconia (normal phase) or polybutadiene (PBD) modified zirconia (reversed phase). In situ micro-diffuse reflectance infrared Fourier transform spectroscopy (μDRIFTS) of the analyte spots is possible due to the low absorption background of both plain and PBD zirconia. An instrument coupling μDRIFTS with a motorized stage that could profile the microchannel TLC plate is developed. The retention order of anthracene and pyrene probes as a function of percent acetonitrile are generally as expected on PBD zirconia but opposite for plain zirconia. Impurities in technical grade methylene blue can be determined after TLC separation on plain zirconia. The separation and infrared identification of various dyes such as dichlorofluorescein and rhodamine B on PBD zirconia is improved using a mobile phase with an additive such as phosphate or dihexylamine that prevents band streaking.
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Kinam, Brigita Olivia Intan, Wisnu Cahyo Prabowo, Supriatno Supriatno, and Rolan Rusli. "Skrining Fitokimia dan Profil KLT Ekstrak dan Fraksi dari Daun Berenuk (Cresentia cujete L.) serta Uji DPPH." Proceeding of Mulawarman Pharmaceuticals Conferences 14 (December 31, 2021): 339–47. http://dx.doi.org/10.25026/mpc.v14i1.600.
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Berenuk or Maja Leaves (Cresentia cujete L.) is a plant that grows in the tropics. In Indonesia, the use of Berenuk Leaves for health is not utilized optimally, and there is still a lack of research on this plant. To be able to be developed as a traditional medicinal ingredient, it is necessary to know the content of secondary metabolites contained in berenuk leaf extract. This study aims to determine the secondary metabolites contained in berenuk leaf extract through phytochemical screening, TLC profile in C. cujete L. leaves. Extraction was carried out using methanol by maceration. After fractionation were carried out by using n-hexane and ethyl acetate as solvents. Then, a phytochemical analysis was screened and a TLC bioautography. Secondary metabolites by the compounds contained in C. cujete L. are alkaloids, tannins, saponins and steroids. C. cujete L. which has antioxidant activity was marked with yellow spots on TLC plates that had been sprayed with DPPH.