Academic literature on the topic 'Voltage-clamp studies'

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Journal articles on the topic "Voltage-clamp studies"

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Claydon, T. W., and D. Fedida. "Voltage clamp fluorimetry studies of mammalian voltage-gated K+ channel gating." Biochemical Society Transactions 35, no. 5 (2007): 1080–82. http://dx.doi.org/10.1042/bst0351080.

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VCF (voltage clamp fluorimetry) provides a powerful technique to observe real-time conformational changes that are associated with ion channel gating. The present review highlights the insights such experiments have provided in understanding Kv (voltage-gated potassium) channel gating, with particular emphasis on the study of mammalian Kv1 channels. Further applications of VCF that would contribute to our understanding of the modulation of Kv channels in health and disease are also discussed.
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Lynn, John W., David H. McCulloh, and Edward L. Chambers. "Voltage clamp studies of fertilization in sea urchin eggs." Developmental Biology 128, no. 2 (1988): 305–23. http://dx.doi.org/10.1016/0012-1606(88)90294-1.

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Schuster, Bernhard, Dietmar Pum, and Uwe B. Sleytr. "Voltage clamp studies on S-layer-supported tetraether lipid membranes." Biochimica et Biophysica Acta (BBA) - Biomembranes 1369, no. 1 (1998): 51–60. http://dx.doi.org/10.1016/s0005-2736(97)00206-x.

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Mleux, Benoit Saint, and L. E. Moore. "Active Dendritic Membrane Properties of XenopusLarval Spinal Neurons Analyzed With a Whole Cell Soma Voltage Clamp." Journal of Neurophysiology 83, no. 3 (2000): 1381–93. http://dx.doi.org/10.1152/jn.2000.83.3.1381.

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Voltage- and current-clamp measurements of inwardly directed currents were made from the somatic regions of Xenopus laevisspinal neurons. Current-voltage ( I-V) curves determined under voltage clamp, but not current clamp, were able to indicate a negative slope conductance in neurons that showed strong accommodating action potential responses to a constant current stimulation. Voltage-clamp I-V curves from repetitive firing neurons did not have a net negative slope conductance and had identical I-V plots under current clamp. Frequency domain responses indicate negative slope conductances with
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Díaz-Ríos, Manuel, Daniel A. Dombeck, Watt W. Webb, and Ronald M. Harris-Warrick. "Serotonin Modulates Dendritic Calcium Influx in Commissural Interneurons in the Mouse Spinal Locomotor Network." Journal of Neurophysiology 98, no. 4 (2007): 2157–67. http://dx.doi.org/10.1152/jn.00430.2007.

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Commissural interneurons (CINs) help to coordinate left–right alternating bursting activity during fictive locomotion in the neonatal mouse spinal cord. Serotonin (5-HT) plays an active role in the induction of fictive locomotion in the isolated spinal cord, but the cellular targets and mechanisms of its actions are relatively unknown. We investigated the possible role of serotonin in modifying dendritic calcium currents, using a combination of two-photon microscopy and patch-clamp recordings, in identified CINs in the upper lumbar region. Dendritic calcium responses to applied somatic voltage
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Bolton, T. B., R. J. Lang, T. Takewaki, and C. D. Benham. "Patch and Whole-Cell Voltage-Clamp Studies on Single Smooth Muscle Cells." Journal of Cardiovascular Pharmacology 8 (1986): S20—S24. http://dx.doi.org/10.1097/00005344-198600088-00005.

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Ye, Q., G. Heck, and J. DeSimone. "The anion paradox in sodium taste reception: resolution by voltage-clamp studies." Science 254, no. 5032 (1991): 724–26. http://dx.doi.org/10.1126/science.1948054.

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Klitzner, Thomas, and William F. Friedman. "Excitation-Contraction Coupling in Developing Mammalian Myocardium: Evidence from Voltage Clamp Studies." Pediatric Research 23, no. 4 (1988): 428–32. http://dx.doi.org/10.1203/00006450-198804000-00018.

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Sims, S. M., and S. J. Dixon. "Inwardly rectifying K+ current in osteoclasts." American Journal of Physiology-Cell Physiology 256, no. 6 (1989): C1277—C1282. http://dx.doi.org/10.1152/ajpcell.1989.256.6.c1277.

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Membrane properties of freshly isolated rat osteoclasts were studied using the whole cell patch-clamp recording technique. The membrane potential could switch between two stable levels, approximately -70 and -15 mV. Voltage-clamp studies indicated that osteoclasts exhibited marked inward rectification, with hyperpolarizing voltage commands from -70 mV activating large inward currents. No voltage-dependent currents were observed in response to depolarization. An increase in external K+ concentration shifted the current-voltage relationship positive in a manner predicted for K+ current. Furtherm
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Zottoli, Steven J. "How the Early Voltage Clamp Studies of José del Castillo Inform “Modern” Neuroscience." Neuroscientist 18, no. 5 (2012): 415–21. http://dx.doi.org/10.1177/1073858412446971.

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Dissertations / Theses on the topic "Voltage-clamp studies"

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Ward, Thomas Anthony. "Voltage and patch clamp studies of the ionic currents in snail neurones and frog skeletal muscle." Thesis, University of Leicester, 1985. http://hdl.handle.net/2381/33620.

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The inactivation of the calcium permeability in Helix aspersa neurones was studied in relation to [Ca]. Injection of calcium ions was shown to reduce the voltage activated calcium current by increasing [Ca]. This was proved to be by a process of inactivation of the calcium current, rather than a direct effect of increased [Ca] on the driving force for calcium across the membrane. [Ca] was measured using ion sensitive electrodes, and a mean resting level of 2.66+/-0.65 x 10-7K was obtained. The inactivation of the calcium current was found to fit 1 to 1 binding between calcium and the receptor
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Rosano, Giulio. "Structure-function studies of TRPV1 alcohol modulation." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2019. http://amslaurea.unibo.it/19298/.

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The TRPV1 channel is a member of the Transient Receptor Potential (TRP) family of cation channels and is responsible for the perception of different noxious stimuli. It is mainly found in the peripheral terminals of primary sensory neurons where pain is produced and in various brain regions such as hypothalamus, cerebellum and cerebralcortex. It can be activated by a large number of physical and chemical stimuli and themost known ones include capsaicin (the active component of hot chili peppers), noxiousheat (greater than 42◦C), voltage and acidity. There is now evi
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Lin, Bor-Ruei. "Electrophysiological Studies on Escherichia coli Protein-conducting Channel." Digital Archive @ GSU, 2008. http://digitalarchive.gsu.edu/biology_diss/52.

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We have developed a novel, sensitive and less time-consuming method to detect activity of the SecA-dependent protein-conducting channels. Nanogram levels of E. coli inverted membrane vesicles were injected into Xenopus oocytes, and ionic currents were recorded using the two-electrode voltage clamp. Currents were observed only in the presence of E. coli SecA in conjunction with E. coli membranes. The observed currents showed outward rectification in the presence of KCl as permeable ions and were significantly enhanced by coinjection with the precursor protein, proOmpA, or active LamB signal pep
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Liang, Guihua. "K⁺ channels in the inner ear : electrophysiological and molecular studies /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7349-971-4/.

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Chui, Jonathan Ka Wang. "A new paradigm for voltage-clamp studies of synthetic ion channels." Thesis, 2011. http://hdl.handle.net/1828/3494.

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Two classes of ion-channels comprising 22 members were prepared. Three members were linear oligo-esters with terephthalate core designed to span both leaflets of the bilayer; these were prepared in a modular synthesis in three linear steps. 19 half-channels based on cyclodextrins with functionalized primary-rims were prepared by the Huisgen Cu+-catalyzed [3+2]-cyclization; three distinct synthetic protocols were established to be applicable to these substrates. The voltage-clamp experiment was used to characterize the ion transport properties of these 22 compounds as well as 5 oligo-esters pre
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Dürr, Katharina Luise [Verfasser]. "Functional significance of Na+,K+- and H+,K+-ATPase β-subunits [beta-subunits] studied by Voltage-Clamp Fluorometry / vorgelegt von Katharina Luise Dürr". 2009. http://d-nb.info/998816698/34.

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Book chapters on the topic "Voltage-clamp studies"

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DeSimone, John A., Krishna C. Persaud, and Gerard L. Heck. "Voltage-Clamp Studies of the Isolated Olfactory Mucosa." In Molecular Neurobiology of the Olfactory System. Springer US, 1988. http://dx.doi.org/10.1007/978-1-4613-0989-5_8.

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Bouteau, François, and Daniel Tran. "Plant Response to Stress: Microelectrode Voltage-Clamp Studies." In Plant Electrophysiology. Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-29119-7_3.

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Kitamura, Kenji, Noriyoshi Teramoto, Masahiro Oike, et al. "Characteristics of the Voltage-Dependent Calcium Channel in Smooth Muscle: Patch-Clamp Studies." In Advances in Experimental Medicine and Biology. Springer US, 1991. http://dx.doi.org/10.1007/978-1-4684-6003-2_18.

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VOGEL, WERNER, and JÜRGEN R. SCHWARZ. "Voltage-clamp studies in axons: Macroscopic and single-channel currents." In The Axon. Oxford University Press, 1995. http://dx.doi.org/10.1093/acprof:oso/9780195082937.003.0013.

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Levitan, Irwin B., and Leonard K. Kaczmarek. "Ion Channels, Membrane Ion Currents, and the Action Potential." In The Neuron. Oxford University Press, 2015. http://dx.doi.org/10.1093/med/9780199773893.003.0006.

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The flow of ions down their electrochemical gradients, through populations of ion channels in the neuronal plasma membrane, gives rise to transmembrane ion currents. It is the sum of the various currents flowing at any point in time that determines the neuron’s membrane potential. Thus the normal firing pattern of a neuron, and its response to different kinds of stimulation, can be seen as a play of interactions among the currents flowing through the different kinds of ion channels in its membrane. The activities of the sodium and potassium channels responsible for axonal action potentials are themselves dependent on voltage. Voltage clamp studies, which allow the measurement of the current flowing through these channels at fixed voltage, have provided a detailed understanding of the sequence of changes in sodium and potassium channel activity that give rise to action potentials.
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Forster, Ian C., and Nikolaus G. Greeff. "An improved voltage clamp for gating current recording from the squid giant axon." In Cephalopod NeurobiologyNeuroscience Studies in Squid, Octopus and Cuttlefish. Oxford University Press, 1995. http://dx.doi.org/10.1093/acprof:oso/9780198547907.003.0070.

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Conference papers on the topic "Voltage-clamp studies"

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Sherman, Christopher, Eli S. Leland, Andrew Pullin, Richard M. White, and Paul K. Wright. "Proximity-Based Passive Current Sensors for Real-Time Monitoring of Power Usage in Low and Mid-Voltage Applications." In ASME 2009 International Mechanical Engineering Congress and Exposition. ASMEDC, 2009. http://dx.doi.org/10.1115/imece2009-11674.

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Traditional monitoring of current flow in power lines involves breaking the circuit for through-the-sensor type monitoring or separating individual wires for around-the-conductor “clamp” metering. Such techniques for measurement are invasive and disruptive for many applications. Using 0.9 cm diameter permanent magnets attached to piezoelectric bimorph elements in conjunction with the fluctuating magnetic fields generated by alternating currents, sensors have been developed that enable in-situ, self-powered measurement of current flow without the need to encircle or disconnect the wire being me
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Nguyen, Mary-Anne, Nima Tamaddoni, and Stephen A. Sarles. "Interrogation of Bilayers in a Multi-Droplet Cluster for Membrane-Based Sensing." In ASME 2015 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/smasis2015-8970.

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The long-term vision of our work is to create a new class of smart material that utilizes networks of active, synthetic cell membranes for sensing, actuation, and energy harvesting. Having multiple membrane structures is specifically targeted because a higher density of functional membranes is expected to enable amplification and collective utility, similar to how living tissues and organisms utilize networks of highly connected cells to accomplish large tasks. While there are several known methods for assembling droplet-based networks of synthetic lipid bilayers, there has been much less effo
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Mojahedi, Mahdi, Keikhosrow Firoozbakhsh, Ahmad Barari, and Mohammad Taghi Ahmadian. "Static and Dynamic Analysis of a Clamp-Clamp Nano-Beam Under Electrostatic Actuation and Detection Considering Intermolecular Forces." In ASME 2013 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/imece2013-62216.

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Micro/nano gyroscopes which can measure angular rate or angle are types of merging gyroscope technology with MEMS/NEMS technology. They have extensively used in many fields of engineering, such as automotive, aerospace, robotics and consumer electronics. There are many studies of a variety of gyroscopes with various drive and detect methods and different resonator structures in last years. In case of electrostatically actuated and detected beam micro/nano-gyroscopes, DC voltages are applied in driving and sensing directions and AC voltage is utilized in driving direction in order to excite dri
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