Bibliografías temáticas / 2D-DIGE ( Two Dimensional Differential In Gel Electrophoresis)

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Literatura académica sobre el tema "2D-DIGE ( Two Dimensional Differential In Gel Electrophoresis)"

Autor: Grafiati
Publicado: 10 de marzo de 2023
Última modificación: 31 de julio de 2025

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Artículos de revistas sobre el tema "2D-DIGE ( Two Dimensional Differential In Gel Electrophoresis)"

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Ercan, Huriye, Ulrike Resch, Felicia Hsu, et al. "A Practical and Analytical Comparative Study of Gel-Based Top-Down and Gel-Free Bottom-Up Proteomics Including Unbiased Proteoform Detection." Cells 12, no. 5 (2023): 747. http://dx.doi.org/10.3390/cells12050747.

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Proteomics is an indispensable analytical technique to study the dynamic functioning of biological systems via different proteins and their proteoforms. In recent years, bottom-up shotgun has become more popular than gel-based top-down proteomics. The current study examined the qualitative and quantitative performance of these two fundamentally different methodologies by the parallel measurement of six technical and three biological replicates of the human prostate carcinoma cell line DU145 using its two most common standard techniques, label-free shotgun and two-dimensional differential gel e
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Oliva, Karen, Gillian Barker, Clyde Riley, et al. "The effect of pre-existing maternal obesity on the placental proteome: two-dimensional difference gel electrophoresis coupled with mass spectrometry." Journal of Molecular Endocrinology 48, no. 2 (2012): 139–49. http://dx.doi.org/10.1530/jme-11-0123.

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Our aim was to study the protein expression profiles of placenta obtained from lean and obese pregnant women with normal glucose tolerance at the time of term Caesarean section. We used two-dimensional difference gel electrophoresis (2D-DIGE), utilising narrow-range immobilised pH gradient strips that encompassed the broad pH range of 4–5 and 5–6, followed by MALDI-TOF mass spectrometry of selected protein spots. Western blot and quantitative RT-PCR (qRT-PCR) analyses were performed to validate representative findings from the 2D-DIGE analysis. Eight proteins were altered (six down-regulated a
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Tajima, Takashi, Fusako Kito, Akihiko Yoshida, Akira Kawai, and Tadashi Kondo. "Calreticulin as A Novel Potential Metastasis-Associated Protein in Myxoid Liposarcoma, as Revealed by Two-Dimensional Difference Gel Electrophoresis." Proteomes 7, no. 2 (2019): 13. http://dx.doi.org/10.3390/proteomes7020013.

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Myxoid liposarcoma (MLS) is a mesenchymal malignancy. To identify innovate seeds for clinical applications, we examined the proteomes of primary tumor tissues from 10 patients with MLS with different statuses of postoperative metastasis. The protein expression profiles of tumor tissues were created, and proteins with differential expression associated with postoperative metastasis were identified by two-dimensional difference gel electrophoresis (2D-DIGE) and mass spectrometry. The validation was performed using specific antibodies and in vitro analyses. Using 2D-DIGE, we observed 1726 protein
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Dhingra, V., Q. Li, A. B. Allison, D. E. Stallknecht, and Z. F. Fu. "Proteomic Profiling and Neurodegeneration in West-Nile-Virus-Infected Neurons." Journal of Biomedicine and Biotechnology 2005, no. 3 (2005): 271–79. http://dx.doi.org/10.1155/jbb.2005.271.

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West Nile virus, a mosquito-borne flavivirus, is a human, equine, and avian pathogen. High-resolution two-dimensional differential-gel electrophoresis (2D-DIGE) was used to characterize protein expression in primary rat neurons and to examine the proteomic profiling to understand the pathogenesis of West-Nile-associated meningoencephalitis. Three pH ranges, 3–10, 4–7, and 5–6, were used to analyze the protein spots. The proteins are labeled with fluorescent dyes Cy3 and Cy5 before being separated on the basis of charge and size respectively on a two-dimensional platform. About 55 proteins show
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Di Carli, Mariasole, Anita Zamboni, Mario Enrico Pè, et al. "Two-Dimensional Differential in Gel Electrophoresis (2D-DIGE) Analysis of Grape Berry Proteome during Postharvest Withering." Journal of Proteome Research 10, no. 2 (2011): 429–46. http://dx.doi.org/10.1021/pr1005313.

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Wang, X., A. Kaya, and E. Memili. "195 SPERMATOZOAL PROTEIN MARKERS FOR ANGUS BULL FERTILITY." Reproduction, Fertility and Development 23, no. 1 (2011): 198. http://dx.doi.org/10.1071/rdv23n1ab195.

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Fertility is one of the most economically important traits controlling animal reproduction. Despite its significant economic impact, there are no reliable markers to predict semen quality. The objective of this study was to identify spermatozoal proteins associated with bull fertility, the ability of the sperm to fertilize the oocyte and support embryonic development. To accomplish our objectives, we isolated total spermatozoal proteins from 4 Angus bulls with different fertility phenotypes. Next, differentially expressed proteins were determined using two-dimensional differential in-gel elect
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Orenes-Piñero, Esteban, Marta Cortón, Pilar González-Peramato, et al. "Searching Urinary Tumor Markers for Bladder Cancer Using a Two-Dimensional Differential Gel Electrophoresis (2D-DIGE) Approach." Journal of Proteome Research 6, no. 11 (2007): 4440–48. http://dx.doi.org/10.1021/pr070368w.

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Subong, Bryan John J., Arturo O. Lluisma, Rhodora V. Azanza, and Lilibeth A. Salvador-Reyes. "Differentiating Two Closely Related Alexandrium Species Using Comparative Quantitative Proteomics." Toxins 13, no. 1 (2020): 7. http://dx.doi.org/10.3390/toxins13010007.

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Alexandrium minutum and Alexandrium tamutum are two closely related harmful algal bloom (HAB)-causing species with different toxicity. Using isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics and two-dimensional differential gel electrophoresis (2D-DIGE), a comprehensive characterization of the proteomes of A. minutum and A. tamutum was performed to identify the cellular and molecular underpinnings for the dissimilarity between these two species. A total of 1436 proteins and 420 protein spots were identified using iTRAQ-based proteomics and 2D-DIGE, resp
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Martínez-Gomariz, M., M. L. HernÁez, D. GutiÉrrez, P. XimÉnez-EmbÚn, and G. PrÉstamo. "Proteomic Analysis by Two-Dimensional Differential Gel Electrophoresis (2D DIGE) of a High-Pressure Effect in Bacillus cereus." Journal of Agricultural and Food Chemistry 57, no. 9 (2009): 3543–49. http://dx.doi.org/10.1021/jf803272a.

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Gemoll, Timo, Svitlana Rozanova, Christian Röder, et al. "Protein Profiling of Serum Extracellular Vesicles Reveals Qualitative and Quantitative Differences after Differential Ultracentrifugation and ExoQuick™ Isolation." Journal of Clinical Medicine 9, no. 5 (2020): 1429. http://dx.doi.org/10.3390/jcm9051429.

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Solid tumor biopsies are the current standard for precision medicine. However, the procedure is invasive and not always feasible. In contrast, liquid biopsies, such as serum enriched for extracellular vesicles (EVs) represent a non-invasive source of cancer biomarkers. In this study, we compared two EV isolation methods in the context of the protein biomarker detection in inflammatory bowel disease (IBD) and colorectal cancer (CRC). Using serum samples of a healthy cohort as well as CRC and IBD patients, EVs were isolated by ultracentrifugation and ExoQuick™ in parallel. EV associated protein
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Tesis sobre el tema "2D-DIGE ( Two Dimensional Differential In Gel Electrophoresis)"

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Guterres, Sheila Barreto. "Busca de biomarcadores para esquizofrenia em plaquetas utilizando eletroforese diferencial em gel bidimensional (2D-DIGE) e espectrometria de massas." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/75/75132/tde-16112011-150931/.

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A esquizofrenia é uma doença crônica, grave e incapacitante que afeta cerca 24 milhões de pessoas em âmbito mundial. É caracterizada por uma desorganização no pensamento que prejudica a funcionalidade do indivíduo. Existem intervenções que são efetivas e contribuem para a diminuição da prevalência do transtorno, pois ajudam o portador a levar uma vida produtiva e integrada à sociedade, porém devem ser ministradas nos estágios iniciais da doença. No entanto, existe uma grande dificuldade em se diagnosticar a esquizofrenia precocemente devido a sua complexidade e às sutilezas dos seus sintomas a
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Kultima, Kim. "Transcriptomics and Proteomics Applied to Developmental Toxicology." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7921.

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Marroncini, Giada. "Ambiente e Genetica: due attori nello sviluppo e progressione del danno epatico." Doctoral thesis, 2018. http://hdl.handle.net/2158/1125023.

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Lo scopo di questo studio è stato quello di indagare i meccanismi dello sviluppo della malattia epatica analizzandone i vari steps che culminano nella cirrosi e nell` epatocarcinoma. Avendo a disposizioni alcuni dati preliminari che individuavano la sirtuina 1 come possibile regolatore della fibrogenesi epatica il mio lavoro ha cercato di approfondire tale argomento. Ho modulato l’attività di questo enzima attraverso l’uso di farmaci inibitori selettivi (EX527) e attivatori (Resveratrolo) per valutare il suo ruolo nella regolazione delle cellule stellate epatiche mediante l’uso di linee cellul
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Capítulos de libros sobre el tema "2D-DIGE ( Two Dimensional Differential In Gel Electrophoresis)"

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Meleady, Paula. "Two-Dimensional Gel Electrophoresis and 2D-DIGE." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2831-7_1.

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Meleady, Paula. "Two-Dimensional Gel Electrophoresis and 2D-DIGE." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7268-5_1.

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Aquino, Adriano, Paul C. Guest, and Daniel Martins-de-Souza. "Simultaneous Two-Dimensional Difference Gel Electrophoresis (2D-DIGE) Analysis of Two Distinct Proteomes." In Multiplex Biomarker Techniques. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6730-8_17.

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Hariharan, Deepak, Mark E. Weeks, and Tatjana Crnogorac-Jurcevic. "Application of Proteomics in Cancer Gene Profiling: Two-Dimensional Difference in Gel Electrophoresis (2D-DIGE)." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-545-9_11.

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Guest, Paul C. "A Two-Dimensional Difference Gel Electrophoresis (2D-DIGE) Protocol for Studies of Neural Precursor Cells." In Advances in Experimental Medicine and Biology. Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-52479-5_14.

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Minden, Jonathan S. "Two-Dimensional Difference Gel Electrophoresis (2D DIGE)." In Methods in Cell Biology. Elsevier, 2012. http://dx.doi.org/10.1016/b978-0-12-405914-6.00006-8.

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"2D-DIGE A Powerful Tool for Proteome Analysis." In Protocols used in Molecular Biology, edited by Sudhir K. Shekhar, Jai Godheja, and Dinesh Raj Modi. BENTHAM SCIENCE PUBLISHERS, 2020. http://dx.doi.org/10.2174/9789811439315120010010.

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In the recent past, two dimensional gel electrophoresis has emerged as a powerful molecular biology tool for the comparative expression profiling of complex protein sample. It involves the separation as well as the resolution of diverse proteins sample on the basis of isoelectric points and molecular mass of protein in two dimension ways. In this way, it reflects the view of overall proteome status including differentiation in protein expression levels, post-translational modifications etc. Moreover, this allows the identification of novel biological signatures, which may give a particular ide
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Actas de conferencias sobre el tema "2D-DIGE ( Two Dimensional Differential In Gel Electrophoresis)"

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Kikuchi, Ryoko, Kei-ichi Iwaya, and Osamu Matsubara. "Abstract 3948: Proteome analysis of ovarian cancer cell lines under hypoxic conditon by two-dimensional difference gel electrophoresis (2D-DIGE)." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-3948.

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