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Tesis sobre el tema "3D cell culture, spheroids"

Autor: Grafiati
Publicado: 4 de junio de 2021
Última modificación: 12 de febrero de 2022

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1

Timmins, Nicholas E. "Extending the third dimension : novel methods and applications for 3D multicellular spheroids /." [St. Lucia, Qld.], 2004. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe18289.pdf.

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2

Pyne, Emily Seton. "The Impact of Stromal Cells on the Metabolism of Ovarian Cancer Cells in 3D Culture." Thesis, Virginia Tech, 2017. http://hdl.handle.net/10919/74931.

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Academic: Ovarian cancer is the leading cause of death among female gynecologic cancers. Current treatments include surgical debulking, and chemotherapy. However, better interventions are needed to reduce the mortality rate of metastatic disease. Ovarian cancer cells have displayed the ability to aggregate and form 3D homogeneous and heterogeneous spheroids, which can function as micrometastases. Ovarian cancer spheroids survive independently prior to adhering to an endothelial tissue. Since aggregation has been shown to provide a survival advantage to the spheroids and increased their aggres
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3

Yang, Mandy. "An investigation of the antifungal and antitumor activity of ajoene." Thesis, University of Canterbury. Biological sciences, 2013. http://hdl.handle.net/10092/8201.

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The garlic extract ajoene is considered to have antimicrobial and antitumor effects against a variety of cell types, and it is suggested to have the potential to be used as an antifungal or antitumor drug clinically. The underlying mechanism of its inhibitory effects is still uncertain. In this project, the effects of ajoene on the growth of fungal and oomycete cells were studied on Candida albicans, Neurospora crassa and Achlya bisexualis. Endometrial cancer is the most common gynecologic cancer. A 3D spheroid model of endometrial cancer cells were for the first time used to i
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4

Kashtl, Ghasaq J. "Differential membrane-type matrix metalloproteinase expression in phenotypically defined breast cancer cell lines: Comparison of MT-MMP expression in environmentally-challenged 2D monolayer cultures and 3D multicellular tumour spheroids." Thesis, University of Bradford, 2018. http://hdl.handle.net/10454/17346.

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Matrix metalloproteinases (MMPs) are a family of zinc endopeptidases capable of digesting the extracellular matrix (ECM), which is essential for tissue structure and transmitting messages between cells. MMPs play an important role in cancer, controlling cell migration, proliferation, apoptosis, regulation of tumour expansion, angiogenesis and invasion. Previous research has indicated high expression of MT1-MMP in breast cancers suggesting a potential role in tumour progression. Our results confirm that 3D multicellular tumour spheroids (MCTS) using phenotype-specific breast cancer cell lines a
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5

Chamayou, Léo. "LiverPearls, une méthode de culture multicellulaire miniaturisée et à haut débit reproduisant l’environnement physiologique et la structure tridimensionnelle du foie humain." Thesis, Université Paris sciences et lettres, 2020. http://www.theses.fr/2020UPSLS005.

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L’intérêt pour de nouveaux modèles de foie, plus proches physiologiquement du foie in vivo, est élevé, en particulier en provenance de l’industrie pharmaceutique. En effet, les systèmes standards, tels que la culture en 2D ne sont pas très prédictifs pour certaines études et de meilleurs modèles sont nécessaires, à la fois dans le cadre des études ADME/Tox pour le développement de médicaments ou pour modéliser les nombreuses maladies hépatiques. Afin de reproduire le foie humain, un modèle doit imiter sa structure de façon plus proche que les systèmes en 2D et refléter sa composition cellulair
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6

Senkowski, Wojciech. "High-throughput screening using multicellular tumor spheroids to reveal and exploit tumor-specific vulnerabilities." Doctoral thesis, Uppsala universitet, Cancerfarmakologi och beräkningsmedicin, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-320598.

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High-throughput drug screening (HTS) in live cells is often a vital part of the preclinical anticancer drug discovery process. So far, two-dimensional (2D) monolayer cell cultures have been the most prevalent model in HTS endeavors. However, 2D cell cultures often fail to recapitulate the complex microenvironments of in vivo tumors. Monolayer cultures are highly proliferative and generally do not contain quiescent cells, thought to be one of the main reasons for the anticancer therapy failure in clinic. Thus, there is a need for in vitro cellular models that would increase predictive value of
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7

Christakou, Athanasia. "Ultrasound-assisted Interactions of Natural Killer Cells with Cancer Cells and Solid Tumors." Doctoral thesis, KTH, Biomedicinsk fysik och röntgenfysik, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-158522.

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In this Thesis, we have developed a microtechnology-based method for culturing and visualizing high numbers of individual cells and cell-cell interactions over extended periods of time. The foundation of the device is a silicon-glass multiwell microplate (also referred as microchip) directly compatible with fluorescence microscopy. The initial microchip design involved thousands of square wells of sizes up to 80 µm, for screening large numbers of cell-cell interactions at the single cell level. Biocompatibility and confinement tests proved the feasibility of the idea, and further investigation
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8

Ohlin, Mathias. "Ultrasonic Fluid and Cell Manipulation." Doctoral thesis, KTH, Biomedicinsk fysik och röntgenfysik, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-166779.

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During the last decade, ultrasonic manipulation has matured into an important tool with a wide range of applications, from fundamental cell biological research to clinical and industrial implementations. The contactless nature of ultrasound makes it possible to manipulate living cells in a gentle way, e.g., for positioning, sorting, and aggregation. However, when manipulating cells using ultrasound, especially using high acoustic amplitudes, a great deal of heat can be generated. This constitutes a challenge, since the viability of cells is dependent on a stable physiological temperature aroun
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9

Dubois, Clémence. "Optimisation du traitement du cancer du sein Triple-Négatif : développement des modèles de culture cellulaire en trois dimensions, efficacité de l'Olaparib (anti-PARP1) en combinaison avec la radiothérapie et chimiorésistance instaurée par les protéines Multi Drug Résistance." Thesis, Université Clermont Auvergne‎ (2017-2020), 2018. http://www.theses.fr/2018CLFAS018/document.

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Le cancer du sein est une maladie complexe et difficile à caractériser. Parmi les différents sous-types moléculaires, les tumeurs du sein Triple-Négatives (TN) sont particulièrement agressives et de mauvais pronostic. Elles sont caractérisées par une absence d’expression des récepteurs aux œstrogènes (ER), à la progestérone (PR), l’absence de surexpression du récepteur Human Epidermal growth factor 2 (HER2) et de fréquentes mutations sur les gènes BRCA1/2 (profil « BRCAness »). En absence de thérapies ciblées efficaces, de nombreux traitements ciblés notamment les inhibiteurs de poly-ADP-ribos
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10

Pasqua, Mattia. "Preclinical studies on an extracorporeal bioartificial liver." Thesis, Compiègne, 2020. http://www.theses.fr/2020COMP2557.

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Pour tous les patients souffrant d'une insuffisance hépatique aigue, il existe un besoin urgent de solutions alternatives à la transplantation du foie. En raison de divers facteurs, ces patients n'ont pas toujours accès à un organe et meurent dans l’attente d’une greffe. Il est donc impératif de trouver des alternatives viables à la transplantation du foie. Parmi les différentes alternatives apparues ces dernières années, notre groupe a principalement étudié le concept de foie bioartificiel. Il s'agit d'un dispositif de circulation extracorporelle équipé d'éléments artificiels (charbon actif e
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11

Liu, Lu. "The effect of hypoxia and 3D culture conditions on heterogeneous ovarian cancer spheroids." Thesis, Virginia Tech, 2017. http://hdl.handle.net/10919/74235.

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Epithelial ovarian cancer (EOC) is the leading cause of death from gynecological malignancy due to the insufficient accurate screening programs for the early detection of EOC. To improve the accuracy of the early detection, there is a need to deeply understand the mechanism of EOC progression and the interaction between cancer cells with their unique microenvironment. Therefore, this work investigated the metabolic shift in the mouse model for progressive ovarian cancer, and evaluated the effects of hypoxic environment, spheroid formation as well as stromal vascular fractions (SVF) on the meta
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12

Löhr, Janine [Verfasser]. "Stem Cell Features in Spheroids and Standard Culture of a Renal Cell Carcinoma Cell Line / Janine Löhr." Berlin : Freie Universität Berlin, 2020. http://d-nb.info/1212435508/34.

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13

Atefi, Ehsan. "Aqueous Biphasic 3D Cell Culture Micro-Technology." University of Akron / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=akron1443112692.

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14

Morrone, Luigi. "The Influence of 3D Cell Organization in Tumor Spheroid on Natural Killer Cell Infiltration and Migration." Thesis, KTH, Skolan för kemi, bioteknologi och hälsa (CBH), 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-286605.

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Natural Killer cells are a type of lymphocyte belonging to the innate immune system and they operate cell-mediated cytotoxicity and release of pro-inflammatory cytokines against cancerous cells. However, in vivo testings have shown a reduced activity of NK cells against solid tumors probably due to the negative influence of the immunosuppressive tumor microenvironment. Multicellular tumor spheroids may constitute an advantageous model in cancer biology for studying the mechanisms behind cancer immune editing since it more closely mimics the complexity of the human body compared with the 2D mod
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15

Ham, Stephanie Lemmo. "Engineering Tumor Models Using Aqueous Biphasic 3D Culture Microtechnology." University of Akron / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=akron150470381711759.

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16

Pasturel, Aurélien. "Tailoring common hydrogels into 3D cell culture templates." Thesis, Bordeaux, 2019. http://www.theses.fr/2019BORD0302.

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L’ingénierie d’hydrogels ; leur structuration et fonctionnalisation à l’échelle cellulaire, est une étape clé pour aboutir à de modèles in-vitro plus physiologiques. À ce jour, elle reste difficile car ces matériaux polymères, mous et riches en eau, sont souvent trop fragiles pour la micro-fabrication traditionnelle. Pour pallier à ce fait, nous avons combinée illumination ultraviolette structurée et chambres de réaction perméables au gaz nous offrant la maitrise sur la distribution de photons, les réactifs et les gaz présents à chaque instant et en tout point d’un champ d’illumination. Nous p
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17

Chetty, Avashnee Shamparkesh. "Thermoresponsive 3D scaffolds for non-invasive cell culture." Thesis, University of Pretoria, 2012. http://hdl.handle.net/2263/25463.

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Conventionally, adherent cells are cultured in vitro using flat 2D cell culture trays. However the 2D cell culture method is tedious, unreliable and does not replicate the complexity of the 3D dynamic environment of native tissue. Nowadays 3D scaffolds can be used to culture cells. However a number of challenges still exist, including the need for destructive enzymes to release confluent cells. Poly(Nisopropylacrylamide) (PNIPAAm), a temperature responsive polymer, has revolutionised the cell culture fraternity by providing a non-invasive means of harvesting adherent cells, whereby confluent c
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18

Tang, Claire. "Self-assembled peptide gels for 3D cell culture." Thesis, University of Manchester, 2010. https://www.research.manchester.ac.uk/portal/en/theses/selfassembled-peptide-gels-for-3d-cell-culture(035b395c-ccaf-4874-b2dc-6330c33db1c5).html.

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Under specific conditions short peptides modified with an N-terminal fluorenyl-9-methoxycarbonyl (Fmoc) group can self-assemble into hydrogel scaffolds similar in properties to the natural extracellular matrix. Fmoc-diphenylalanine (Fmoc-FF) for instance, has been shown to form hydrogels at physiological pH that have the ability to support 2D and 3D cell culture. The aim of this investigation is to provide further understanding of the self-assembly mechanism of such systems in order to progress towards the establishment of design rules for the preparation of scaffolds with tuneable properties.
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19

Aladdad, Afnan. "Dynamic patterned electrospun fibres for 3D cell culture." Thesis, University of Nottingham, 2016. http://eprints.nottingham.ac.uk/33895/.

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Current culture methods to generate large quantities of cells destined for tissue engineering and regenerative medicine commonly use enzymatic digestion. However, this method is not desirable for subsequent cell transfer to the body due to the destruction of important cell-surface proteins and the risk of enzymatic contamination [1]. Therefore, research has led to the development of thermo-responsive surfaces for the continued culture of mammalian cells, with passaging achieved via a drop in the culture temperature. Recognising that the three-dimensional (3D) culture environment influences the
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20

Papas, Klearchos Kyriacos. "Characterization of the metabolic and secretory behavior of suspended free and entrapped AtT-20 spheroids in fed-batch and perfusion cultures." Thesis, Georgia Institute of Technology, 1992. http://hdl.handle.net/1853/11002.

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21

Sprague, Leslee W. "Dendritic Cell Culture With 2D and 3D Collagen Substrates." Ohio University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1311616312.

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22

Zhao, Huizhi. "3D Cell Culture Model Synthesized By Polycaprolactone Nanofiber Electrospinning." Ohio University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1531319675295094.

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23

Marin, Paya Juan Carlos. "3D Culture o Multiple Myeloma Cell Line Using Microgel Environments." Doctoral thesis, Universitat Politècnica de València, 2021. http://hdl.handle.net/10251/167427.

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[ES] El mieloma múltiple es una neoplasia hematológica caracterizada por una expansión descontrolada de células plasmáticas monoclonales (mPCs) en medula ósea que producen, en la mayoría de los casos, un componente monoclonal secretado en el suero y/o en orina. En la actualidad, se sigue considerando una enfermedad incurable con la constante aparición de recaídas en los pacientes. Una de las causas que condicionan esta situación, radica en la generación de resistencia frente a fármacos por parte de las mPCs. Este mecanismo de resistencia a fármacos (DR) se ha visto que no solo depende de facto
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24

Berdeu, Anthony. "Imagerie sans lentille 3D pour la culture cellulaire 3D." Thesis, Université Grenoble Alpes (ComUE), 2017. http://www.theses.fr/2017GREAS036/document.

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Ce travail de thèse se situe à l’interface de deux domaines : la culture cellulaire en trois dimensions et l’imagerie sans lentille.Fournissant un protocole de culture cellulaire plus réaliste sur le plan physiologique, le passage des cultures monocouches (2D) à des cultures tridimensionnelles (3D) - via l’utilisation de gels extracellulaires dans lesquels les cellules peuvent se développer dans les trois dimensions - permet de faire de grandes avancées dans de nombreux domaines en biologie tels que l’organogénèse, l’oncologie et la médecine régénérative. Ces nouveaux objets à étudier crée un
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25

Nakajima, Aya. "Radiation sensitivity assay with a panel of patient-derived spheroids of small cell carcinoma of the cervix." Kyoto University, 2015. http://hdl.handle.net/2433/199178.

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26

Capra, J. (Janne). "Differentiation and malignant transformation of epithelial cells:3D cell culture models." Doctoral thesis, Oulun yliopisto, 2018. http://urn.fi/urn:isbn:9789526218236.

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Abstract The epithelial cells form barriers that compartmentalize the organs. Carcinomas are cancers stemming from epithelial cells and are the most common cancer type. The aim of this study was to understand the differentiation and malignant transformation of epithelial Madin-Darby canine kidney (MDCK) cells and to analyse the electrophysiological parameters which regulate their transport capacity. Emphasis was placed on comparing different culture environments, both in 2D and 3D. First, the effects of drugs or basal extracellular fluid composition on MDCK cell, cyst and lumen volumes were an
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27

Marrazzo, Pasquale <1986&gt. "Evaluation of 3D cell culture systems for host-pathogen interaction studies." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2015. http://amsdottorato.unibo.it/7010/.

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Traditional cell culture models have limitations in extrapolating functional mechanisms that underlie strategies of microbial virulence. Indeed during the infection the pathogens adapt to different tissue-specific environmental factors. The development of in vitro models resembling human tissue physiology might allow the replacement of inaccurate or aberrant animal models. Three-dimensional (3D) cell culture systems are more reliable and more predictive models that can be used for the meaningful dissection of host–pathogen interactions. The lung and gut mucosae often represent the first site o
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28

Amaral, Jonatas Bussador do. "Células MCF-7 como modelo 3D no estudo de câncer de mama humano." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/42/42134/tde-21072011-134443/.

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O diferencial da cultura de células em 3-dimensões é permitir que as células explorem as 3-dimensões do espaço, aumentando assim as interações com o ambiente e entre as células. Em estudos relacionados à biologia do câncer de mama, vem ganhando espaço a utilização de esferóides para estudos que visam à compreensão da morfogênese do espaço luminal. Neste trabalho foi mostrado que as células MCF-7 reorganizam-se em estruturas tubulares e acinares. Em ambas as situações, a formação do lúmen veio acompanhada pelo estabelecimento de uma camada de células polarizadas, arranjo este muito semelhante a
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29

Tomasi, Raphaël. "Multiscale cytometry of 3D cell cultures in microfluidic hydrogel arrays." Thesis, Université Paris-Saclay (ComUE), 2016. http://www.theses.fr/2016SACLX114/document.

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Les conditions du corps humain ne sont pas reproduites fidèlement par la culture cellulaire traditionnelle en 2D. Dans cette thèse, des cultures cellulaires 3D sont réalisées dans une plateforme microfluidique hautement intégrée. Des cellules mammifères adhérentes sont encapsulées dans des gouttes immobilisées dans un tableau de pièges capillaires à haute densité. Dans chaque goutte, les cellules se réorganisent pour former un unique microtissu 3D et fonctionnel appelé sphéroïde. L'utilisation d'un hydrogel permet d'alonger le temps de culture et de perfuser le tableau avec des solutions aqueu
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30

Walker, Matthew. "Dynamic Mechanical Regulation of Cells in 3D Microtissues." Thesis, Université d'Ottawa / University of Ottawa, 2020. http://hdl.handle.net/10393/40555.

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It has been well established that the fundamental behaviors of mammalian cells are influenced by the physical cues that they experience from their surrounding environment. With respect to cells in our bodies, mechanically-driven morphological and phenotypic changes to our cells have been linked to responses critical to both normal development and disease progression, including lung, heart, muscle and bone disorders, and cancer. Although significant advancements to our understanding of cell behavior have been made using 2D cell culture methods, questions regarding how physical stretch guides ce
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31

Dohns, Edgardo Abelardo. "Rationally designed proteinogenic hydrogels as extracellular matrix mimics for 3D cell culture." Thesis, University of Bristol, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.573928.

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Rationally designed polypeptide-based materials have advantages over other biomaterials for their ability to be engineered from the bottom-up, and to be tailored to the needs of specific potential applications. Based on previous works in the Woolfson laboratory on self- assembling peptide fibres (SAF), this research project focused on developing coiled-coil- based fibrillar hydrogel networks called hydrogelating SAFs (hSAFs), and applying them to more-complex biological systems, notably in cell culture. The hSAFs were tested as a three- dimensional cell-culture scaffold with the aim of mimicki
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32

Mahboubian, AliReza. "Development of thermoresponsive polymeric hydrogels as in vitro 3D cell culture matrices." Thesis, University of Nottingham, 2015. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.727120.

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Historically, the examinations of cell and tissue physiology and pathophysiology have been carried out in 2D cell cultures outside of the human body. However, recently, it has been demonstrated that the functionality of cells cultured in two dimensions is very different to that of similar cells cultured in three dimensional cell cultures. Also cells cultured in three dimensions can represent better the native tissue. Therefore, it is important to create 3D growth conditions that can mimic a native tissue closely in vitro. Some 3D models have been developed, however, they are typically costly t
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33

Venturato, Andrea. "2D and 3D applications of polymeric biomaterials." Thesis, University of Edinburgh, 2018. http://hdl.handle.net/1842/31045.

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The field of biomaterials has seen huge development over the past decade with enormous efforts invested in discovering materials with improved biocompatibility, application and versatility. Polymers can display many properties that make them ideal biomaterials, such as their potential flexibility, low weight, low cost and biodegradability. Moreover, they can be prepared in a wide variety of compositions and forms and be readily fabricated into various shapes and structures. Polymer microarrays represent an efficient high-throughput platform for the screening and discovery of new materials comp
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34

Bell, Alex E. "Microscale Additive Manufacturing of Collagen Cell Culture Scaffolds." University of Cincinnati / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1448037063.

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35

Höfner, Christiane [Verfasser], Torsten [Gutachter] Blunk, Matthias [Gutachter] Becker, and Norbert [Gutachter] Schütze. "Human Adipose-derived Mesenchymal Stem Cells in a 3D Spheroid Culture System - Extracellular Matrix Development, Adipogenic Differentiation, and Secretory Properties / Christiane Höfner ; Gutachter: Torsten Blunk, Matthias Becker, Norbert Schütze." Würzburg : Universität Würzburg, 2020. http://d-nb.info/121086231X/34.

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36

Thomas, Robert James. "Formation, morphology and function of a 3D hepatocyte-hepatic stellate cell co-culture system." Thesis, University of Nottingham, 2006. http://eprints.nottingham.ac.uk/11964/.

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In these studies the ability of a three-dimensional hepatocyte-stellate cell co-culture system to preserve some key aspects of differentiated hepatocyte function in vitro is demonstrated. A poly(DL-lactic Acid) surface allows dynamic and rapid interaction of hepatocytes and stellate cells to form co-culture spheroids in a complex multistage process (shown by time lapse microscopy). After five days the spheroids have developed a substantial extracellular matrix support and hepatic ultra-structure including bile canaliculi, tight junctions, desmosomes and lipid storage. The distribution of the s
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37

Bruyneel, Arne A. N. "Stem cell culture on 3D-scaffolds for cardiac regenerative medicine and in vitro studies." Thesis, University of Oxford, 2015. https://ora.ox.ac.uk/objects/uuid:b9c1aac3-7328-4322-8cc6-0d5667fe944a.

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Most life years lost due to death and disability in the Western world arise from non-communicable diseases, such as cancer and cardiovascular disease. Myocardial infarction (MI) and heart failure (HF) still have a particular poor prognosis, despite the development of improved pharmacological therapy and surgical interventions. No cure, other than heart transplant, is available for patients with HF. As a result, further research into new treatment strategies is paramount. Many potential avenues have been suggested, but this thesis aimed to work towards using stem cells and tissue engineering st
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38

Dolega, Monika Elzbieta. "Developement of microtechnologies for 3D cell culture to study prostate acini formation and carcinogenesis." Thesis, Grenoble, 2014. http://www.theses.fr/2014GRENS022/document.

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Tout épithélium glandulaire sécrétoire est constitué d'une unité structurale et fonctionnelle commune, l'acinus. C'est une architecture sphérique pluricellulaire parfaitement différentiée et polarisée qui, reconstruite en culture 3D, mime l'organisation réelle du tissu. Etudier les déterminants environnementaux et génétiques qui gouvernent la transformation d'un acinus en sphéroïde s'apparentant à une tumeur est l'un des enjeux majeurs des modèles in vitro. Un des défis actuels est d'adapter ces modèles in vitro à des conditions de culture 3D qui soient compatibles avec la réalisation de cribl
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39

Shi, Xinglong. "DIFFERENTIATION OF NEURAL STEM CELL USING SMALL MOLECULES IN 2D AND 3D CULTURE SYSTEM." Master's thesis, Temple University Libraries, 2015. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/363660.

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Bioengineering<br>M.S.<br>The neuronal differentiation of neural stem cells (NSCs) has received much attention due to its potential for the treatment of neurodegenerative diseases (i.e., Parkinson’s and Alzheimer’s diseases). In this regard, discovering compounds that direct differentiation of NSCs is highly required to facilitate therapeutic applications. In this study, we examined various bioactive compounds (SA1, SA2, LiCl, compound B, and DHED) to induce the neuronal differentiation of human neural stem cells (hNSCs). The study was conducted on the cells grown in three dimensional (3D) hyd
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40

Li, Quan. "Synthetic Hydrogel-Based 3D Culture System for Maintenance of Human Induced Pluripotent Stem Cell." Thesis, Kansas State University, 2017. http://hdl.handle.net/2097/36189.

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Master of Science<br>Department of Grain Science and Industry<br>X. Susan Sun<br>Human induced pluripotent stem cells (hiPSCs) are generated from human somatic cells using defined transcription factors. These cells possess characteristics very similar to that of human embryonic stem cells including the ability to differentiate into cell types of all three germ layers. HiPSCs show great potential in clinical researches like drug screening and regenerative medicine, that all require large amount of cells cultured under well-defined conditions. The most common culture methods used for hiPSCs are
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41

Dumbleton, Jenna K. "Miniaturized 3D culture of stem cells with biomaterials derived from alginate." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1431029490.

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42

Zhu, Dan [Verfasser]. "Investigations towards 2D and 3D cell culture devices based on nanocrystalline diamond layers / Dan Zhu." Ulm : Universität Ulm, 2016. http://d-nb.info/1103572911/34.

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43

Vickerman, Vernella V. V. (Vernella Velonie Verlin). "Microfluidic-based 3D cell culture for studies of biophysical and biochemical regulation of endothelial function." Thesis, Massachusetts Institute of Technology, 2012. http://hdl.handle.net/1721.1/76487.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 2012.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (p. 210-229).<br>New and more biologically relevant in vitro models are needed for use in drug development, regenerative medicine, and fundamental scientific investigations. The ultimate challenge lies in replicating the native cell/tissue environment ex vivo. Certain key features of living tissues such as the three dimensionality, biophysical and biochemical microenvironment cannot be readily replicated in traditional cult
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44

Modulevsky, Daniel. "Plant Derived Cellulose Scaffolds as a Novel Biomaterial for 3D Cell Culture and Tissue Regeneration." Thesis, Université d'Ottawa / University of Ottawa, 2021. http://hdl.handle.net/10393/42184.

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This work presents an alternative approach to the production of cellulose-based biomaterials. Instead of extracting, processing and regenerating plant and or bacteria-derived cellulose into a biomaterial, my work established a decellularization protocol to remove cellular plant content from plant tissue resulting in a scaffold composed of cellulose with the evolved architecture of the plant cell wall. Tracheophyte plants, including clubmosses, horsetails, and ferns, gymnosperms and angiosperms, have evolved distinct vascular structures that support the transport of water and nutrients in xyle
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45

Liang, Wanjun [Verfasser]. "Polyglycerol-based, Chemically Defined Artificial Stem Cell Scaffold Engineering for iPSCs’ 3D Culture / Wanjun Liang." Berlin : Freie Universität Berlin, 2021. http://d-nb.info/1239645627/34.

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46

Huang, Haishui. "Microfluidic Generation and Manipulation of Hydrogel Microcapsules for Biomimetic 3D Tissue Culture and Cell Cryopreservation." The Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu1461095928.

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47

Westergren, Elisabeth. "Analysis of hydrogels for immobilisation of hepatocytes (HepG2) in 3D cell culturing systems." Thesis, Linköpings universitet, Teknisk biologi, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-145392.

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In pharmaceutical development cell cultures are used as in vitro models to evaluate the function of drug candidates. In such research it is vital to have models that resemble the in vivo environment to get reliable results. In 3D models with hydrogels ECM like scaffolds are supporting the cells in a more in vivo like environment than flat 2D cultures. In this project PEG-peptide based hydrogels with cell binding RGD incorporated on one PEG-peptide type has been evaluated for culturing of HepG2 cells. Structure and viscoelastic properties were evaluated with techniques like circular dichroism s
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48

Schmittlein, Carina [Verfasser], and Joachim [Akademischer Betreuer] Wegener. "Sensors and Actuators for 2D and 3D Cell Culture Models based on Oxygen Sensitive Culture Substrates / Carina Schmittlein ; Betreuer: Joachim Wegener." Regensburg : Universitätsbibliothek Regensburg, 2018. http://d-nb.info/1170955738/34.

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49

Cahill, Fiona. "The role of LKB1 (STK11) in non-small cell lung cancer." Thesis, University of Oxford, 2017. https://ora.ox.ac.uk/objects/uuid:a3162d1b-96d3-4420-82eb-e261c9732f33.

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LKB1 is the second most commonly altered tumour suppressor gene in lung adenocarcinoma, the most prevalent form of lung cancer. LKB1 is a "master kinase" that has been shown to phosphorylate up to 13 downstream targets. We hypothesised that LKB1 loss is associated with an increased dependency on alternative, targetable pathways. The overall aims of this project were to better understand the role of LKB1 loss in lung cancer and to identify novel approaches to selectively target LKB1 mutated cells. We generated isogenic cells with or without LKB1 and used these to study the effect of LKB1 on cel
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Senthivel, Vivek Raj 1983. "Engineering ultrasensivity and differential diffusion to build spatial patterns in a 3D mammalian cell culture system." Doctoral thesis, Universitat Pompeu Fabra, 2017. http://hdl.handle.net/10803/482223.

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Engineering self-organised spatial patterns based on reaction-diffusion models can be the stepping stone for tissue engineering. Previous reports based on both theoretical and experimental studies have implied the importance of ultrasensitivity and differential diffusion as key requirements for building self-organised spatial patterns on mammalian cells with extracellular signalling systems. In the sender-receiver system developed by our team using Madin-Darby canine kidney (MDCK) cysts and hepatocyte growth factor (HGF) signalling, I have explored the sensitivity of different genes to varying
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