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Artículos de revistas sobre el tema "3D cell culture, spheroids"

Autor: Grafiati

Publicado: 4 de junio de 2021

Última modificación: 12 de febrero de 2022

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1

Cesarz, Zoe, and Kenichi Tamama. "Spheroid Culture of Mesenchymal Stem Cells." Stem Cells International 2016 (2016): 1–11. http://dx.doi.org/10.1155/2016/9176357.

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Compared with traditional 2D adherent cell culture, 3D spheroidal cell aggregates, or spheroids, are regarded as more physiological, and this technique has been exploited in the field of oncology, stem cell biology, and tissue engineering. Mesenchymal stem cells (MSCs) cultured in spheroids have enhanced anti-inflammatory, angiogenic, and tissue reparative/regenerative effects with improved cell survival after transplantation. Cytoskeletal reorganization and drastic changes in cell morphology in MSC spheroids indicate a major difference in mechanophysical properties compared with 2D culture. E

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Srisongkram, Tarapong, Natthida Weerapreeyakul, and Kanjana Thumanu. "Evaluation of Melanoma (SK-MEL-2) Cell Growth between Three-Dimensional (3D) and Two-Dimensional (2D) Cell Cultures with Fourier Transform Infrared (FTIR) Microspectroscopy." International Journal of Molecular Sciences 21, no. 11 (2020): 4141. http://dx.doi.org/10.3390/ijms21114141.

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Fourier transform infrared (FTIR) microspectroscopy was used to evaluate the growth of human melanoma cells (SK-MEL-2) in two-dimensional (2D) versus three-dimensional (3D) spheroid culture systems. FTIR microspectroscopy, coupled with multivariate analysis, could be used to monitor the variability of spheroid morphologies prepared from different cell densities. The characteristic shift in absorbance bands of the 2D cells were different from the spectra of cells from 3D spheroids. FTIR microspectroscopy can also be used to monitor cell death similar to fluorescence cell staining in 3D spheroid

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Lee, Dongjin, and Chaenyung Cha. "The Combined Effects of Co-Culture and Substrate Mechanics on 3D Tumor Spheroid Formation within Microgels Prepared via Flow-Focusing Microfluidic Fabrication." Pharmaceutics 10, no. 4 (2018): 229. http://dx.doi.org/10.3390/pharmaceutics10040229.

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Tumor spheroids are considered a valuable three dimensional (3D) tissue model to study various aspects of tumor physiology for biomedical applications such as tissue engineering and drug screening as well as basic scientific endeavors, as several cell types can efficiently form spheroids by themselves in both suspension and adherent cell cultures. However, it is more desirable to utilize a 3D scaffold with tunable properties to create more physiologically relevant tumor spheroids as well as optimize their formation. In this study, bioactive spherical microgels supporting 3D cell culture are fa

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Shrestha, Sunil, Vinod Kumar Reddy Lekkala, Prabha Acharya, Darshita Siddhpura, and Moo-Yeal Lee. "Recent advances in microarray 3D bioprinting for high-throughput spheroid and tissue culture and analysis." Essays in Biochemistry 65, no. 3 (2021): 481–89. http://dx.doi.org/10.1042/ebc20200150.

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Abstract Three-dimensional (3D) cell culture in vitro has proven to be more physiologically relevant than two-dimensional (2D) culture of cell monolayers, thus more predictive in assessing efficacy and toxicity of compounds. There have been several 3D cell culture techniques developed, which include spheroid and multicellular tissue cultures. Cell spheroids have been generated from single or multiple cell types cultured in ultralow attachment (ULA) well plates and hanging droplet plates. In general, cell spheroids are formed in a relatively short period of culture, in the absence of extracellu

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Kitano, Otome, and Kohji Nakazawa. "Neuronal Differentiation of NT2 Cells in Monolayer and Spheroid Cultures." MATEC Web of Conferences 333 (2021): 07008. http://dx.doi.org/10.1051/matecconf/202133307008.

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Metabolism and differentiation of cultured cells are influenced by changes in cellular morphology. In this study, we investigated the differences in cell proliferation and neuronal differentiation of NT2 cells in monolayer (2D) and spheroid (3D) cultures. In the monolayer culture, the cells adhered and extended on a tissue culture plate. For the spheroid culture, we fabricated a microwell chip comprising 195 circular microwells (600 ìm in diameter) on a cutture plate, and the surface was modified with polyethylene glycol to promote spheroid formation. The cells were aggregated in each microwel

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Kitano, Otome, and Kohji Nakazawa. "Neuronal Differentiation of NT2 Cells in Monolayer and Spheroid Cultures." MATEC Web of Conferences 333 (2021): 07008. http://dx.doi.org/10.1051/matecconf/202133307008.

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Metabolism and differentiation of cultured cells are influenced by changes in cellular morphology. In this study, we investigated the differences in cell proliferation and neuronal differentiation of NT2 cells in monolayer (2D) and spheroid (3D) cultures. In the monolayer culture, the cells adhered and extended on a tissue culture plate. For the spheroid culture, we fabricated a microwell chip comprising 195 circular microwells (600 ìm in diameter) on a cutture plate, and the surface was modified with polyethylene glycol to promote spheroid formation. The cells were aggregated in each microwel

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Son, Young-Bum, Dinesh Bharti, Saet-Byul Kim, et al. "Comparison of Pluripotency, Differentiation, and Mitochondrial Metabolism Capacity in Three-Dimensional Spheroid Formation of Dental Pulp-Derived Mesenchymal Stem Cells." BioMed Research International 2021 (July 13, 2021): 1–10. http://dx.doi.org/10.1155/2021/5540877.

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Mesenchymal stem cells (MSCs) are valuable candidates in tissue engineering and stem cell-based therapy. Traditionally, MSCs derived from various tissues have been successfully expanded in vitro using adherent culture plates commonly called as monolayer two-dimensional (2D) cultures. Recently, many studies demonstrated that stemness and multilineage differentiation potential could be enhanced to greater extent when MSCs are cultured as suspended aggregates by means of three-dimensional (3D) culturing techniques. However, there are limited reports on changed mitochondrial metabolism on 3D spher

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Borzenok, S. A., I. A. Popov, I. N. Saburina, and P. M. Arbukhanova. "IN VITRO INVESTIGATION OF THE TRANSPLANTATION PROSPECTS OF MULTICELLULAR SPHEROID MICROAGGREGATES OF DONOR RETINAL PIGMENT EPITHELIUM." Russian Journal of Transplantology and Artificial Organs 17, no. 3 (2015): 58–64. http://dx.doi.org/10.15825/1995-1191-2015-3-58-64.

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Aim. To study in experiment the criteria for transplantability of multicellular spheroid microaggregates of retinal pigment epithelium (RPE), prepared by the method of 3D cell culture. Materials and Methods. 11 donor eyes (6 of adrenaline index «A», 5 of index «B») were used as a source of RPE cell cultures (group «A» – 6 cultures, group «B» – 5 cultures), of which over 2000 RPE spheroids were obtained by the method of three-dimensional cell culture. 1760 spheroids of them were selected for transplantability investigation (960 – group «A», 800 – group «B»). Among the selected spheroids were eq

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Ko, J. Y., E. Lee, J. Kim, and G. I. Im. "THU0058 ENHANCEMENT OF CARTILAGE REGENERATION EFFICIENCY WITH HUMAN ADIPOSE STEM CELL THREE-DIMENSIONAL SPHEROID." Annals of the Rheumatic Diseases 79, Suppl 1 (2020): 241.2–241. http://dx.doi.org/10.1136/annrheumdis-2020-eular.4022.

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Background:3D (three-dimensional) cell culture technology has been researched steadily because of its high potential of biocompatibility compared to single cells since 1990s, and is being developed to 3D spheroids recently. Spheroids are considered to reflect the natural organization of cells better than 2D cell cultures, and stem cells spheroids have been studied extensively in therapeutic transplantation. Stem cells were considered as a method of replacing autologous chondrocyte in regenerative treatment of articular cartilage. Compared to conventional single cells, 3D cell culture is artifi

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Lee, Mason A., Kensey N. Bergdorf, Courtney J. Phifer, et al. "Novel three-dimensional cultures provide insights into thyroid cancer behavior." Endocrine-Related Cancer 27, no. 2 (2020): 111–21. http://dx.doi.org/10.1530/erc-19-0374.

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Thyroid cancer has the fastest growing incidence of any cancer in the United States, as measured by the number of new cases per year. Despite advances in tissue culture techniques, a robust model for thyroid cancer spheroid culture is yet to be developed. Using eight established thyroid cancer cell lines, we created an efficient and cost-effective 3D culture system that can enhance our understanding of in vivo treatment response. We found that all eight cell lines readily form spheroids in culture with unique morphology, size, and cytoskeletal organization. In addition, we developed a high-thr

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Min, Tae-Jun, Min Ji Kim, Kyung-Jung Kang, Yeoung Jo Jeoung, Se Heang Oh, and Young-Joo Jang. "3D Spheroid Formation Using BMP-Loaded Microparticles Enhances Odontoblastic Differentiation of Human Dental Pulp Stem Cells." Stem Cells International 2021 (August 23, 2021): 1–12. http://dx.doi.org/10.1155/2021/9326298.

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Human dental pulp stem cells (hDPSCs) are the primary cells responsible for dentin regeneration. Typically, in order to allow for odontoblastic differentiation, hDPSCs are cultured over weeks with differentiation-inducing factors in a typical monolayered culture. However, monolayered cultures have significant drawbacks including inconsistent differentiation efficiency, require a higher BMP concentration than should be necessary, and require periodic treatment with BMPs for weeks to see results. To solve these problems, we developed a 3D-cell spheroid culture system for odontoblastic differenti

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Ivascu, Andrea, and Manfred Kubbies. "Rapid Generation of Single-Tumor Spheroids for High-Throughput Cell Function and Toxicity Analysis." Journal of Biomolecular Screening 11, no. 8 (2006): 922–32. http://dx.doi.org/10.1177/1087057106292763.

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Spheroids are widely used in biology because they provide an in vitro 3-dimensional (3D) model to study proliferation, cell death, differentiation, and metabolism of cells in tumors and the response of tumors to radiotherapy and chemotherapy. The methods of generating spheroids are limited by size heterogeneity, long cultivation time, or mechanical accessibility for higher throughput fashion. The authors present a rapid method to generate single spheroids in suspension culture in individual wells. A defined number of cells ranging from 1000 to 20,000 were seeded into wells of poly-HEMA-coated,

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Choi, So-Young, Soo Hyun Kang, Su Young Oh, et al. "Differential Angiogenic Potential of 3-Dimension Spheroid of HNSCC Cells in Mouse Xenograft." International Journal of Molecular Sciences 22, no. 15 (2021): 8245. http://dx.doi.org/10.3390/ijms22158245.

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The experimental animal model is still essential in the development of new anticancer drugs. We characterized mouse tumors derived from two-dimensional (2D) monolayer cells or three-dimensional (3D) spheroids to establish an in vivo model with highly standardized conditions. Primary cancer-associated fibroblasts (CAFs) were cultured from head and neck squamous cell carcinoma (HNSCC) tumor tissues and co-injected with monolayer cancer cells or spheroids into the oral mucosa of mice. Mice tumor blood vessels were stained, followed by tissue clearing and 3D Lightsheet fluorescent imaging. We comp

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Grayson, Korie A., Nidhi Jyotsana, Nerymar Ortiz-Otero, and Michael R. King. "Overcoming TRAIL-resistance by sensitizing prostate cancer 3D spheroids with taxanes." PLOS ONE 16, no. 3 (2021): e0246733. http://dx.doi.org/10.1371/journal.pone.0246733.

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Three-dimensional spheroid cultures have been shown to better physiologically mimic the cell-cell and cell-matrix interactions that occur in solid tumors more than traditional 2D cell cultures. One challenge in spheroid production is forming and maintaining spheroids of uniform size. Here, we developed uniform, high-throughput, multicellular spheroids that self-assemble using microwell plates. DU145 and PC3 cells were cultured as 2D monolayers and 3D spheroids to compare sensitization of TRAIL-resistance cancer cells to TRAIL mediated apoptosis via chemotherapy based on dimensionality. Monocul

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Boyer, Jean Zheng, Gail D. Lewis Phillips, Hiro Nitta, et al. "Activity of trastuzumab emtansine (T-DM1) in 3D cell culture." Breast Cancer Research and Treatment 188, no. 1 (2021): 65–75. http://dx.doi.org/10.1007/s10549-021-06272-x.

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Abstract Background Cell spheroids and aggregates generated from three-dimensional (3D) cell culture methods are similar to in vivo tumors in terms of tissue morphology, biology, and gene expression, unlike cells grown in 2D cell cultures. Breast cancer heterogeneity is one of the main drug resistant mechanisms and needs to be overcome in order to increase the efficacy of drug activity in its treatments. Methods We performed a unique 3D cell culture and drug efficacy study with trastuzumab emtansine (Kadcyla®, T-DM1) across five breast cancer cell lines (BT-474, SK-BR-3, MDA-MB-361, MDA-MB-175

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Herheliuk, Tetiana, Olena Perepelytsina, Natalia Yurchenko, Mykhailo Sydorenko, and Lyudmila Ostapchenko. "EXPRESSION OF TUMOR ASSOSIATED AND EPITHELIAL-MESENCHYMAL TRANSITION MARKERS IN 2D AND 3D CELL CULTURES OF MCF-7." EUREKA: Health Sciences 6 (November 30, 2016): 37–44. http://dx.doi.org/10.21303/2504-5679.2016.00231.

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The target effects on the expression of epithelial-mesenchymal transition regulation molecules are promising for cancer therapy, including breast cancer. 3D cell culture is a model for studying epithelial-mesenchymal transition in vitro and may become a test system for anticancer therapy. Aim of research. The aim of this research was to evaluate and compare the expression of tumor associated and epithelial-mesenchymal transition markers in tumor cells of breast adenocarcinoma (MCF-7 cell line) in 2D and 3D cell culture. Methods. For realization of the aim MCF-7 cell line (breast adenocarcinoma

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Lim, Wanyoung, and Sungsu Park. "A Microfluidic Spheroid Culture Device with a Concentration Gradient Generator for High-Throughput Screening of Drug Efficacy." Molecules 23, no. 12 (2018): 3355. http://dx.doi.org/10.3390/molecules23123355.

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Three-dimensional (3D) cell culture is considered more clinically relevant in mimicking the structural and physiological conditions of tumors in vivo compared to two-dimensional cell cultures. In recent years, high-throughput screening (HTS) in 3D cell arrays has been extensively used for drug discovery because of its usability and applicability. Herein, we developed a microfluidic spheroid culture device (μFSCD) with a concentration gradient generator (CGG) that enabled cells to form spheroids and grow in the presence of cancer drug gradients. The device is composed of concave microwells with

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Xie, Lili, Mao Mao, Liang Zhou, Lusi Zhang, and Bing Jiang. "Signal Factors Secreted by 2D and Spheroid Mesenchymal Stem Cells and by Cocultures of Mesenchymal Stem Cells Derived Microvesicles and Retinal Photoreceptor Neurons." Stem Cells International 2017 (2017): 1–13. http://dx.doi.org/10.1155/2017/2730472.

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We aim to identify levels of signal factors secreted by MSCs cultured in 2D monolayers (2D-MSCs), spheroids (spheroids MSCs), and cocultures of microvesicles (MVs) derived from 2D-MSCs or spheroid MSCs and retinal photoreceptor neurons. We seeded 2D-MSCs, spheroid MSCs, and cells derived from spheroids MSCs at equal numbers. MVs isolated from all 3 culture conditions were incubated with 661W cells. Levels of 51 signal factors in conditioned medium from those cultured conditions were quantified with bead-based assay. We found that IL-8, IL-6, and GROαwere the top three most abundant signal fact

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N’deh, Kaudjhis Patrick Ulrich, Gyeong-Ji Kim, Kang-Hyun Chung, et al. "Surface-Modified Industrial Acrylonitrile Butadiene Styrene 3D Scaffold Fabrication by Gold Nanoparticle for Drug Screening." Nanomaterials 10, no. 3 (2020): 529. http://dx.doi.org/10.3390/nano10030529.

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Biocompatibility is very important for cell growth using 3D printers, but biocompatibility materials are very expensive. In this study, we investigated the possibility of cell culture by the surface modification of relatively low-cost industrial materials and an efficient three-dimensional (3D) scaffold made with an industrial ABS filament for cell proliferation, spheroid formation, and drug screening applications. We evaluated the adequate structure among two-layer square shape 3D scaffolds printed by fused deposition modeling with variable infill densities (10–50%). Based on the effects of t

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Restle, Luciana, Daniela Costa-Silva, Emanuelle Stellet Lourenço, et al. "A 3D OsteoblastIn VitroModel for the Evaluation of Biomedical Materials." Advances in Materials Science and Engineering 2015 (2015): 1–8. http://dx.doi.org/10.1155/2015/268930.

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Biomedical materials for bone therapy are usually assessed for their biocompatibility and safety employing animal models orin vitromonolayer cell culture assays. However, alternativein vitromodels may offer controlled conditions closer to physiological responses and reduce animal testing. In this work, we developed a 3D spheroidal cell culture with potential to evaluate simultaneously material-cell and cell-cell interactions. Different cell densities of murine MC3T3-E1 preosteoblasts or human primary osteoblasts (HOb) were used to determine the ideal procedure of spheroidal cultures and their

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Kaminska, Agnieszka, Aleksandra Wedzinska, Marta Kot, and Anna Sarnowska. "Effect of Long-Term 3D Spheroid Culture on WJ-MSC." Cells 10, no. 4 (2021): 719. http://dx.doi.org/10.3390/cells10040719.

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The aim of our work was to develop a protocol enabling a derivation of mesenchymal stem/stromal cell (MSC) subpopulation with increased expression of pluripotent and neural genes. For this purpose we used a 3D spheroid culture system optimal for neural stem cells propagation. Although 2D culture conditions are typical and characteristic for MSC, under special treatment these cells can be cultured for a short time in 3D conditions. We examined the effects of prolonged 3D spheroid culture on MSC in hope to select cells with primitive features. Wharton Jelly derived MSC (WJ-MSC) were cultured in

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Fröhlich, Eleonore. "Issues with Cancer Spheroid Models in Therapeutic Drug Screening." Current Pharmaceutical Design 26, no. 18 (2020): 2137–48. http://dx.doi.org/10.2174/1381612826666200218094200.

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In vitro screening for anti-cancer agents currently uses mainly cell lines in 2D culture. It is generally assumed that 3D culture, namely spheroids, represents physiologically more relevant models for tumors. Unfortunately, drug testing in spheroids is not as easy and reproducible as in 2D culture because there are factors that limit the universal use of spheroids as screening platforms. Technical problems in the generation of uniform spheroids, cell/tumor-specific differences in the ability to form spheroids, and more complex readout parameters are the main reasons for differences between sph

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Gögele, Clemens, Christina Hoffmann, Jens Konrad, et al. "Cyclically stretched ACL fibroblasts emigrating from spheroids adapt their cytoskeleton and ligament-related expression profile." Cell and Tissue Research 384, no. 3 (2021): 675–90. http://dx.doi.org/10.1007/s00441-021-03416-9.

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AbstractMechanical stress of ligaments varies; hence, ligament fibroblasts must adapt their expression profile to novel mechanomilieus to ensure tissue resilience. Activation of the mechanoreceptors leads to a specific signal transduction, the so-called mechanotransduction. However, with regard to their natural three-dimensional (3D) microenvironment cell reaction to mechanical stimuli during emigrating from a 3D spheroid culture is still unclear. This study aims to provide a deeper understanding of the reaction profile of anterior cruciate ligament (ACL)-derived fibroblasts exposed to cyclic

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Borzenok, S. A., M. Kh Khubetsova, I. N. Saburina, et al. "Cellular neuroprotection as a modern treatment approach for optic neuropathy." Russian Journal of Transplantology and Artificial Organs 19, no. 1 (2017): 63–73. http://dx.doi.org/10.15825/1995-1191-2017-1-63-73.

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Aim.To develop technology to create 3D-spheroid multipotent mesenchymal stem cells (MMSC) of limbal cadaveric human eyes, capable of safe and long-term secretion of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF).Materials and methods.MMSC were obtained by cultivation of limbal fragments, released from cadaveric donor human eye. Cultivation was carried out in DMEM/F12 medium, supplemented with L-glutamine, penicillin, streptomycin, amphotericin B, HEPES, insulin, dexamethasone and 10 vol.% FBS under standard conditions (5% СО2, 37 °C), medium change was performed every 3

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Wassmer, Charles-Henri, Kevin Bellofatto, Lisa Perez, et al. "Engineering of Primary Pancreatic Islet Cell Spheroids for Three-dimensional Culture or Transplantation: A Methodological Comparative Study." Cell Transplantation 29 (January 1, 2020): 096368972093729. http://dx.doi.org/10.1177/0963689720937292.

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Three-dimensional (3D) cell culture by engineering spheroids has gained increasing attention in recent years because of the potential advantages of such systems over conventional two-dimensional (2D) tissue culture. Benefits include the ability of 3D to provide a more physiologically relevant environment, for the generation of uniform, size-controlled spheroids with organ-like microarchitecture and morphology. In recent years, different techniques have been described for the generation of cellular spheroids. Here, we have compared the efficiency of four different methods of islet cell aggregat

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Balmaña, Meritxell, Stefan Mereiter, Francisca Diniz, Tália Feijão, Cristina Barrias, and Celso Reis. "Multicellular Human Gastric Cancer Spheroids Mimic the Glycosylation Phenotype of Gastric Carcinomas." Molecules 23, no. 11 (2018): 2815. http://dx.doi.org/10.3390/molecules23112815.

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Cellular glycosylation plays a pivotal role in several molecular mechanisms controlling cell–cell recognition, communication, and adhesion. Thus, aberrant glycosylation has a major impact on the acquisition of malignant features in the tumor progression of patients. To mimic these in vivo features, an innovative high-throughput 3D spheroid culture methodology has been developed for gastric cancer cells. The assessment of cancer cell spheroids’ physical characteristics, such as size, morphology and solidity, as well as the impact of glycosylation inhibitors on spheroid formation was performed a

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Dong, Guoyi, Shengpeng Wang, Yuping Ge, et al. "Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation." Stem Cells International 2019 (October 15, 2019): 1–12. http://dx.doi.org/10.1155/2019/6041816.

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Human mesenchymal stem cells (hMSCs) are widely used in clinical research because of their multipotential, immunomodulatory, and reparative properties. Previous studies determined that hMSC spheroids from a three-dimensional (3D) culture possess higher therapeutic efficacy than conventional hMSCs from a monolayer (2D) culture. To date, various 3D culture methods have been developed to form hMSC spheroids but most of them used culture medium containing fetal bovine serum (FBS), which is not suitable for further clinical use. Here, we demonstrate that dissociated single MSCs seeded in induced pl

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Nie, Yan, Xun Xu, Weiwei Wang, Nan Ma, and Andreas Lendlein. "Spheroid formation of human keratinocyte: Balancing between cell-substrate and cell-cell interaction." Clinical Hemorheology and Microcirculation 76, no. 2 (2020): 329–40. http://dx.doi.org/10.3233/ch-209217.

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BACKGROUND: The formation of spheroids is tightly regulated by intrinsic cell-cell and cell-substrate interactions. OBJECTIVE: The chitosan (CS)-coating was applied to investigate the driven force directed the spheroid formation. METHODS: The effects of CS on cell functions were studied. Atomic force microscopy was employed to measure the cell- biomaterial interplay at single cell level. RESULTS: HaCaT cells shifted from their flattened sheet to a compact 3D spheroidal morphology when increasing CS-coating concentration. The proliferative capacity of HaCaT was preserved in the spheroid. The ex

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Svablova, T., N. Ternerova, M. Houdova Megova, P. Busek, and A. Sedo. "P10.01 Effect of mesenchymal cells on infiltration and phenotype of T cells in a 3D glioblastoma spheroid model." Neuro-Oncology 23, Supplement_2 (2021): ii27—ii28. http://dx.doi.org/10.1093/neuonc/noab180.094.

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Abstract BACKGROUND Glioblastoma (GBM), the most malignant primary brain tumor with a median survival less than two years, is characterized by aggressive invasive growth, presence of hypoxic and necrotic regions, and T-cell dysfunction. Mesenchymal cells with characteristics of cancer associated fibroblasts are present in GBMs, but it remains to be established whether they contribute to T cell sequestration in the tumor stroma and functional inactivation similarly to extracranial tumors. Murine models do not reflect several aspects of human immune system and 2D cell culture systems often fail

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Han, Hao-Wei, Shigetaka Asano, and Shan-hui Hsu. "Cellular Spheroids of Mesenchymal Stem Cells and Their Perspectives in Future Healthcare." Applied Sciences 9, no. 4 (2019): 627. http://dx.doi.org/10.3390/app9040627.

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Intrinsic cellular properties of several types of cells are dramatically altered as the culture condition shifts from two-dimensional (2D) to three-dimensional (3D) environment. Currently, several lines of evidence have demonstrated the therapeutic potential of mesenchymal stem cells (MSCs) in regenerative medicine. MSCs not only replenish the lost cells, they also promote the regeneration of impaired tissues by modulating the immune responses. Following the development of 3D cell culture, the enhanced therapeutic efficacy of spheroid-forming MSCs have been identified in several animal disease

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Kozhina, K. V., E. N. Volkova, I. N. Saburina, et al. "The influence of peptide bioregulators on skin aging in 3D culture model." Russian Journal of Skin and Venereal Diseases 19, no. 1 (2016): 58–63. http://dx.doi.org/10.18821/1560-9588-2016-19-1-58-63.

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He effect of mesotherapy injection (Meso-Wharton R199TM) on the dermal fibroblasts culture, simulating condition of (mature) aging skin cells are studied. Material and methods. The culture of 4th passage fibroblasts (P4), that corresponds to young skin fibroblasts (control) and the culture of 18th passage fibroblasts (P18), that has all the signs of aging dermal fibroblasts (predominance of large cells, slow cell division) were used. Bioactivity was assessed by cell morphology, epithelium-mesenchyme plasticity and expression of fibroblasts markers: cytokeratin 19, elastin, a-smooth muscle acti

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Sargenti, Azzurra, Francesco Musmeci, Carola Cavallo, et al. "A new method for the study of biophysical and morphological parameters in 3D cell cultures: Evaluation in LoVo spheroids treated with crizotinib." PLOS ONE 16, no. 6 (2021): e0252907. http://dx.doi.org/10.1371/journal.pone.0252907.

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Three-dimensional (3D) culture systems like tumor spheroids represent useful in vitro models for drug screening and more broadly for cancer biology research, but the generation of uniform populations of spheroids remains challenging. The possibility to properly characterize spheroid properties would increase the reliability of these models. To address this issue different analysis were combined: i) a new device and relative analytical method for the accurate, simultaneous, and rapid measurement of mass density, weight, and size of spheroids, ii) confocal imaging, and iii) protein quantificatio

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Schmal, Olga, Jan Seifert, Tilman E. Schäffer, Christina B. Walter, Wilhelm K. Aicher, and Gerd Klein. "Hematopoietic Stem and Progenitor Cell Expansion in Contact with Mesenchymal Stromal Cells in a Hanging Drop Model Uncovers Disadvantages of 3D Culture." Stem Cells International 2016 (2016): 1–13. http://dx.doi.org/10.1155/2016/4148093.

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Efficientex vivoexpansion of hematopoietic stem cells with a concomitant preservation of stemness and self-renewal potential is still an unresolved ambition. Increased numbers of methods approaching this issue using three-dimensional (3D) cultures were reported. Here, we describe a simplified 3D hanging drop model for the coculture of cord blood-derived CD34+hematopoietic stem and progenitor cells (HSPCs) with bone marrow-derived mesenchymal stromal cells (MSCs). When seeded as a mixed cell suspension, MSCs segregated into tight spheroids. Despite the high expression of niche-specific extracel

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St-Georges-Robillard, Amélie, Maxime Cahuzac, Benjamin Péant, et al. "Long-term fluorescence hyperspectral imaging of on-chip treated co-culture tumour spheroids to follow clonal evolution." Integrative Biology 11, no. 4 (2019): 130–41. http://dx.doi.org/10.1093/intbio/zyz012.

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Abstract Multicellular tumour spheroids are an ideal in vitro tumour model to study clonal heterogeneity and drug resistance in cancer research because different cell types can be mixed at will. However, measuring the individual response of each cell population over time is challenging: current methods are either destructive, such as flow cytometry, or cannot image throughout a spheroid, such as confocal microscopy. Our group previously developed a wide-field fluorescence hyperspectral imaging system to study spheroids formed and cultured in microfluidic chips. In the present study, two subclo

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Mišković Špoljarić, Katarina, Marijana Jukić, Teuta Opačak-Bernardi, and Ljubica Glavaš-Obrovac. "3D Cell Technology in Biomedical Research." Collegium antropologicum 44, no. 3 (2020): 171–74. http://dx.doi.org/10.5671/ca.44.3.10.

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Traditional two dimensional cell culture has enabled great strides in biomedicine but needs to be improved to be able to keep up with the demands of modern biomedical research. 2D monolayer culture cannot replicate tissue responses and needs to be supplemented with extensive animal research. Growing cells in three dimensional scaffolds provides a more functional model for biomedical research than traditional monolayer culture. Depending on the needs and the complexity of the model there are several ways that 3D models can be initiated. Simple spheroids can be grown in low adherence plates and

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Leary, Elizabeth, Claire Rhee, Benjamin T. Wilks, and Jeffrey R. Morgan. "Quantitative Live-Cell Confocal Imaging of 3D Spheroids in a High-Throughput Format." SLAS TECHNOLOGY: Translating Life Sciences Innovation 23, no. 3 (2018): 231–42. http://dx.doi.org/10.1177/2472630318756058.

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Accurately predicting the human response to new compounds is critical to a wide variety of industries. Standard screening pipelines (including both in vitro and in vivo models) often lack predictive power. Three-dimensional (3D) culture systems of human cells, a more physiologically relevant platform, could provide a high-throughput, automated means to test the efficacy and/or toxicity of novel substances. However, the challenge of obtaining high-magnification, confocal z stacks of 3D spheroids and understanding their respective quantitative limitations must be overcome first. To address this

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Dubois, Clémence, Pierre Daumar, Corinne Aubel, et al. "The New Synthetic Serum-Free Medium OptiPASS Promotes High Proliferation and Drug Efficacy Prediction on Spheroids from MDA-MB-231 and SUM1315 Triple-Negative Breast Cancer Cell Lines." Journal of Clinical Medicine 8, no. 3 (2019): 397. http://dx.doi.org/10.3390/jcm8030397.

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Triple-negative breast cancers are particularly aggressive. In vitro cultures are one of the major pathways for developing anticancer strategies. The effectiveness and reproducibility of the drug screenings depend largely on the homogeneity of culture media. In order to optimize the predictive responses of triple-negative breast cancer 3D cell culture models, these works were focused on the development of SUM1315 and MDA-MB-231 cell lines in OptiPASS medium, a new serum-free formulation (BIOPASS). In monolayer cell culture, OptiPASS medium was more suitable for MDA-MB-231 than SUM1315 cell lin

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Liu, Xiaoli, Huichao Lin, Jiaao Song, et al. "A Novel SimpleDrop Chip for 3D Spheroid Formation and Anti-Cancer Drug Assay." Micromachines 12, no. 6 (2021): 681. http://dx.doi.org/10.3390/mi12060681.

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Cell culture is important for the rapid screening of anti-cancer drug candidates, attracting intense interest. Traditional 2D cell culture has been widely utilized in cancer biological research. However, 3D cellular spheroids are able to recapitulate the in vivo microenvironment of tissues or tumors. Thus far, several 3D cell culture methods have been developed, for instance, the hanging drop method, spinner flasks and micropatterned plates. Nevertheless, these methods have been reported to have some disadvantages, for example, medium replacement is inconvenient or causes cellular damage. Here

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Ishikawa, Shohei, Kazutoshi Iijima, Kohei Sasaki, Masaaki Kawabe, and Hidenori Otsuka. "Improvement of Hepatic Functions by Spheroids Coculture with Fibroblasts in 3D Silica Nonwoven Fabrics." Journal of Nanoscience and Nanotechnology 19, no. 6 (2019): 3326–33. http://dx.doi.org/10.1166/jnn.2019.16103.

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In order to realize organ-on-a-chip as an effective tool for regenerative medicine and drug development, tissue-mimic cell culture methods which promote liver-specific function for long period have been developed. We have previously demonstrated that coculture of hepatocyte spheroids on fibroblasts using micropatterned substrate improved the hepatic functions due to the heterotypic cell–cell interactions and paracrine signaling from each other. In addition, hepatocyte function cultured as monolayer was also promoted in separately coculture with fibroblasts cultured as monolayer, and it is more

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Miyamoto, Yoshitaka, Masashi Ikeuchi, Hirofumi Noguchi, Tohru Yagi, and Shuji Hayashi. "Enhanced Adipogenic Differentiation of Human Adipose-Derived Stem Cells in an in vitro Microenvironment: The Preparation of Adipose-Like Microtissues Using a Three-Dimensional Culture." Cell Medicine 9, no. 1-2 (2017): 35–44. http://dx.doi.org/10.3727/215517916x693096.

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The application of stem cells for cell therapy has been extensively studied in recent years. Among the various types of stem cells, human adipose tissue-derived stem cells (ASCs) can be obtained in large quantities with relatively few passages, and they possess a stable quality. ASCs can differentiate into a number of cell types, such as adipose cells and ectodermal cells. We therefore focused on the in vitro microenvironment required for such differentiation and attempted to induce the differentiation of human stem cells into microtissues using a microelectromechanical system. We first evalua

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Białkowska, Kamila, Piotr Komorowski, Maria Bryszewska, and Katarzyna Miłowska. "Spheroids as a Type of Three-Dimensional Cell Cultures—Examples of Methods of Preparation and the Most Important Application." International Journal of Molecular Sciences 21, no. 17 (2020): 6225. http://dx.doi.org/10.3390/ijms21176225.

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Cell cultures are very important for testing materials and drugs, and in the examination of cell biology and special cell mechanisms. The most popular models of cell culture are two-dimensional (2D) as monolayers, but this does not mimic the natural cell environment. Cells are mostly deprived of cell–cell and cell–extracellular matrix interactions. A much better in vitro model is three-dimensional (3D) culture. Because many cell lines have the ability to self-assemble, one 3D culturing method is to produce spheroids. There are several systems for culturing cells in spheroids, e.g., hanging dro

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Eroğlu, Erdal. "In vitro 3D Spheroid Culture Developed on the Parafilm Surface Using HEK-293 Cells." Academic Perspective Procedia 3, no. 1 (2020): 220–27. http://dx.doi.org/10.33793/acperpro.03.01.48.

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Preclinical research to predict the effects of drugs and chemicals on humans is commonly carried out either by cell culture studies in vitro condition or on animals in vivo condition. While drug studies tested on cells cultured as a monolayer in plastic flasks are incompatible with realistic results, falsifying findings can also be achieved from in vivo studies performed on different species. In recent years, research on drug tests using spheroid cultures formed by growing cells in three-dimensional (3D) in vitro has attracted great interest. 3D spheroid structures are formed by growing the ce

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Uchida, Satoshi, Kayoko Yanagihara, Akitsugu Matsui, Kazunori Kataoka, and Keiji Itaka. "mRNA as a Tool for Gene Transfection in 3D Cell Culture for Future Regenerative Therapy." Micromachines 11, no. 4 (2020): 426. http://dx.doi.org/10.3390/mi11040426.

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A combination of three-dimensional (3D) cell culturing and non-viral gene transfection is promising in improving outcomes of cell transplantation therapy. Herein, gene transfection profiles in 3D cell culture were compared between plasmid DNA (pDNA) and messenger RNA (mRNA) introduction, using mesenchymal stem cell (MSC) 3D spheroids. Green fluorescence protein (GFP) mRNA induced GFP protein expression in 77% of the cells in the spheroids, whereas only 34% of the cells became GFP positive following pDNA introduction. In mechanistic analyses, most of the cells in MSC spheroids were non-dividing

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Xie, Bailu, Jan Hänsel, Vanessa Mundorf, et al. "Pseudopterosin and O-Methyltylophorinidine Suppress Cell Growth in a 3D Spheroid Co-Culture Model of Pancreatic Ductal Adenocarcinoma." Bioengineering 7, no. 2 (2020): 57. http://dx.doi.org/10.3390/bioengineering7020057.

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Current therapies for treating pancreatic ductal adenocarcinoma (PDAC) are largely ineffective, with the desmoplastic environment established within these tumors being considered a central issue. We established a 3D spheroid co-culture in vitro model using a PDAC cell line (either PANC-1 or Capan-2), combined with stellate cells freshly isolated from pancreatic tumors (PSC) or hepatic lesions (HSC), and human type I collagen to analyze the efficiency of the chemotherapeutic gemcitabine (GEM) as well as two novel drug candidates derived from natural products: pseudopterosin (PsA-D) and O-methyl

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Bartosh, Thomas J., and Joni H. Ylostalo. "Efficacy of 3D Culture Priming is Maintained in Human Mesenchymal Stem Cells after Extensive Expansion of the Cells." Cells 8, no. 9 (2019): 1031. http://dx.doi.org/10.3390/cells8091031.

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The use of non-optimal preparations of mesenchymal stem cells (MSCs), such as extensively expanded cells, might be necessary to obtain the large numbers of cells needed for many clinical applications. We previously demonstrated that minimally expanded (early passage) MSCs can be pre-activated as spheroids to produce potentially therapeutic factors in 3D cultures. Here, we used extensively expanded (late passage) MSCs and studied their 3D-culture activation potential. MSCs were culture-expanded as 2D monolayers, and cells from various passages were activated by 3D culture in hanging drops with

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Ott, Lindsey M., Karthik Ramachandran, and Lisa Stehno-Bittel. "An Automated Multiplexed Hepatotoxicity and CYP Induction Assay Using HepaRG Cells in 2D and 3D." SLAS DISCOVERY: Advancing the Science of Drug Discovery 22, no. 5 (2017): 614–25. http://dx.doi.org/10.1177/2472555217701058.

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Drug-induced liver injury (DILI) and drug–drug interactions (DDIs) are concerns when developing safe and efficacious compounds. We have developed an automated multiplex assay to detect hepatotoxicity (i.e., ATP depletion) and metabolism (i.e., cytochrome P450 1A [CYP1A] and cytochrome P450 3A4 [CYP3A4] enzyme activity) in two-dimensional (2D) and three-dimensional (3D) cell cultures. HepaRG cells were cultured in our proprietary micromold plates and produced spheroids. HepaRG cells, in 2D or 3D, expressed liver-specific proteins throughout the culture period, although 3D cultures consistently

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Nguyen, Lam-Huyen. "ID:2051 Generation of Microtumors Using 3D Hanging drop culture system and breast cancer cell MCF7 for testing anticancer drug response." Biomedical Research and Therapy 4, S (2017): 81. http://dx.doi.org/10.15419/bmrat.v4is.285.

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Cancer is one of the most leading causes of death all over the world. Great deal of effort has been made to find out new agents for cancer treatment. Cancer cell models for drug testing play a critical role in this process. In recent years, 3D cell models were proved to be more effective mimic structure and character of cancer cells in tumors than 2D cell culture, hence they present the effect of drug more accurate to in vivo result. Vietnam which is a tropical country has tremendous potential in cancer drug screening due to its diversity of herbals. However, 3D cancer cell model for testing t

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Sim, Jooyoung, Hyun Jung Lee, Byeongmoon Jeong, and Min Hee Park. "Poly(Ethylene Glycol)-Poly(l-Alanine)/Hyaluronic Acid Complex as a 3D Platform for Understanding Cancer Cell Migration in the Tumor Microenvironment." Polymers 13, no. 7 (2021): 1042. http://dx.doi.org/10.3390/polym13071042.

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Cancer progression and migration in the tumor microenvironment are related to cell types and three-dimensional (3D) matrices. Therefore, developing biomimetic tumor models, including co-culture systems and a tunable 3D matrix, could play an essential role in understanding the cancer environment. Here, multicellular spheroids using human adipose-derived mesenchymal stem cells (hADSCs) and breast cancer cells (MDA-MB-231) within the 3D matrix were used as a tumor microenvironment (TME) mimicking platform. The amphiphilic peptide block copolymer and hyaluronic acid (HA) formed a self-assembled st

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Zhang, Xuan, Ming-Gen Hu, Ke Pan, Chong-Hui Li, and Rong Liu. "3D Spheroid Culture Enhances the Expression of Antifibrotic Factors in Human Adipose-Derived MSCs and Improves Their Therapeutic Effects on Hepatic Fibrosis." Stem Cells International 2016 (2016): 1–8. http://dx.doi.org/10.1155/2016/4626073.

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Three-dimensional (3D) cell culture has been reported to increase the therapeutic potentials of mesenchymal stem cells (MSCs). However, the action mechanisms of 3D MSCs vary greatly and are far from being thoroughly investigated. In this study, we aimed to investigate the therapeutic effects of 3D spheroids of human adipose-derived MSCs for hepatic fibrosis. Our results showed that 3D culture enhanced the expression of antifibrotic factors by MSCs, including insulin growth factor 1 (IGF-1), interleukin-6 (IL-6), and hepatocyte growth factor (HGF).In vitrostudies indicated conditioned medium of

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Hundsberger, Harald, Anna Stierschneider, Victoria Sarne, et al. "Concentration-Dependent Pro- and Antitumor Activities of Quercetin in Human Melanoma Spheroids: Comparative Analysis of 2D and 3D Cell Culture Models." Molecules 26, no. 3 (2021): 717. http://dx.doi.org/10.3390/molecules26030717.

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Quercetin, a dietary flavonoid found in fruits and vegetables, has been described as a substance with many anti-cancer properties in a variety of preclinical investigations. In the present study, we demonstrate that 2D and 3D melanoma models exhibit not only different sensitivities to quercetin, but also opposite, cancer-promoting effects when metastatic melanoma spheroids are treated with quercetin. Higher concentrations of quercetin reduce melanoma growth in three tested cell lines, whereas low concentrations induce the opposite effect in metastatic melanoma spheroids but not in the non-meta

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