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1

Hu, Ming Ming, and Shu Xu. "Discussion on the Contents of 5S Activities in Universities." Advanced Materials Research 219-220 (March 2011): 1427–30. http://dx.doi.org/10.4028/www.scientific.net/amr.219-220.1427.

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Regarding the shortcomings of 5S activities mainly carried out by local in universities, this article puts forward the idea of the full implementation of 5S activities within the universities. From the classroom 5S, dormitories 5S, library 5S, laboratory 5S, etc, we made researches on the contents of 5S activities in universities, which can provide a better reference for the implementation of 5S activities in universities.
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2

Kmiec, E. B., and A. Worcel. "The positive transcription factor of the 5S RNA gene proteolyses during direct exchange between 5S DNA sites." Journal of Cell Biology 103, no. 3 (1986): 673–81. http://dx.doi.org/10.1083/jcb.103.3.673.

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We have examined the association, dissociation, and exchange of the 5S specific transcription factor (TFIIIA) with somatic- and oocyte-type 5S DNA. The factor associates faster with somatic than with oocyte 5S DNA, and the rate of complex formation is accelerated by vector DNA. Once formed, the TFIIIA-5S DNA complex is stable for greater than 4 h in the absence of free 5S DNA, and its dissociation is identical for somatic and for oocyte 5S DNA. In the presence of free 5S DNA, the factor transfers promptly from the complex to the free 5S DNA site. Unexpectedly, the direct exchange of factor bet
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3

Randhawa, Jugraj Singh, and Inderpreet Singh Ahuja. "5S – a quality improvement tool for sustainable performance: literature review and directions." International Journal of Quality & Reliability Management 34, no. 3 (2017): 334–61. http://dx.doi.org/10.1108/ijqrm-03-2015-0045.

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Purpose The purpose of this paper is to present the comprehensive literature review on 5S and suggest possible gaps from the point of view of researchers and practitioners. The manuscript presents the overview of 5S implementation and highlights the achievements realized from deployment of 5S initiatives for sustainable performance of organizations. Design/methodology/approach The paper critically examines the literature on 5S, analyzes and reviews it systematically. The study highlights the critical barriers and success factors for sustainable 5S implementation in the organizations in the com
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4

Chipev, C. C., and A. P. Wolffe. "Chromosomal organization of Xenopus laevis oocyte and somatic 5S rRNA genes in vivo." Molecular and Cellular Biology 12, no. 1 (1992): 45–55. http://dx.doi.org/10.1128/mcb.12.1.45-55.1992.

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We describe the chromosomal organization of the major oocyte and somatic 5S RNA genes of Xenopus laevis in chromatin isolated from erythrocyte nuclei. Both major oocyte and somatic 5S DNA repeats are associated with nucleosomes; however, differences exist in the organization of chromatin over the oocyte and somatic 5S RNA genes. The repressed oocyte 5S RNA gene is protected from nuclease digestion by incorporation into a nucleosome, and the entire oocyte 5S DNA repeat is assembled into a loosely positioned array of nucleosomes. In contrast, the potentially active somatic 5S RNA gene is accessi
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5

Chipev, C. C., and A. P. Wolffe. "Chromosomal organization of Xenopus laevis oocyte and somatic 5S rRNA genes in vivo." Molecular and Cellular Biology 12, no. 1 (1992): 45–55. http://dx.doi.org/10.1128/mcb.12.1.45.

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We describe the chromosomal organization of the major oocyte and somatic 5S RNA genes of Xenopus laevis in chromatin isolated from erythrocyte nuclei. Both major oocyte and somatic 5S DNA repeats are associated with nucleosomes; however, differences exist in the organization of chromatin over the oocyte and somatic 5S RNA genes. The repressed oocyte 5S RNA gene is protected from nuclease digestion by incorporation into a nucleosome, and the entire oocyte 5S DNA repeat is assembled into a loosely positioned array of nucleosomes. In contrast, the potentially active somatic 5S RNA gene is accessi
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6

Pereira da Silva, Amanda, Amanda Pereira da Luz, Catiussa Maiara Pazuch, and Lisandra Maria Kovaliczn Nadal. "Programa 5S e suas contribuições para instituições de ensino com caráter social." Revista Tecnologia e Sociedade 19, no. 58 (2023): 144. http://dx.doi.org/10.3895/rts.v19n58.16143.

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Oriunda no Japão, a ferramenta 5S é um método para implantação de conceitos de gestão da qualidade em organizações. Apesar disso, ainda são escassas aplicações em instituições de ensino de cunho social, haja visto que pode atuar como fator de melhoria de vida da comunidade e construção de conhecimento. O objetivo principal foi realizar um levantamento dos trabalhos publicados que relacionam o Programa 5S e instituições de ensino de cunho social para comparar com as ações extensionistas desenvolvidas pelas autoras. Para o levantamento bibliográfico foram selecionados trabalhos acadêmicos que ap
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7

Bogenhagen, D. F. "Proteolytic footprinting of transcription factor TFIIIA reveals different tightly binding sites for 5S RNA and 5S DNA." Molecular and Cellular Biology 13, no. 9 (1993): 5149–58. http://dx.doi.org/10.1128/mcb.13.9.5149-5158.1993.

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Transcription factor IIIA (TFIIIA) employs an array of nine N-terminal zinc fingers to bind specifically to both 5S RNA and 5S DNA. The binding of TFIIIA to 5S RNA and 5S DNA was studied by using a protease footprinting technique. Brief treatment of free or bound TFIIA with trypsin or chymotrypsin generated fragments which were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Fragments retaining the N terminus of TFIIA were identified by immunoblotting with an antibody directed against the N terminus of TFIIIA. Proteolytic footprinting of TFIIIA complexed with 5S DNA der
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8

Bogenhagen, D. F. "Proteolytic footprinting of transcription factor TFIIIA reveals different tightly binding sites for 5S RNA and 5S DNA." Molecular and Cellular Biology 13, no. 9 (1993): 5149–58. http://dx.doi.org/10.1128/mcb.13.9.5149.

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Transcription factor IIIA (TFIIIA) employs an array of nine N-terminal zinc fingers to bind specifically to both 5S RNA and 5S DNA. The binding of TFIIIA to 5S RNA and 5S DNA was studied by using a protease footprinting technique. Brief treatment of free or bound TFIIA with trypsin or chymotrypsin generated fragments which were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Fragments retaining the N terminus of TFIIA were identified by immunoblotting with an antibody directed against the N terminus of TFIIIA. Proteolytic footprinting of TFIIIA complexed with 5S DNA der
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9

Nogaroto, Viviane, Larissa Glugoski, Orlando Moreira Filho, and Marcelo Ricardo Vicari. "rDNA 5S e rearranjos cromossômicos em Rineloricaria (Siluriformes: Loricariidae)." Semina: Ciências Biológicas e da Saúde 38, no. 1supl (2018): 230. http://dx.doi.org/10.5433/1679-0367.2017v38n1suplp230.

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Dados citogenéticos de Loricariidae (Siluriformes) revelam uma extensiva variação de número diploide e plasticidade cromossômica no grupo. Nestes peixes, a localização in situ de sítios de rDNA 5S em regiões co-localizadas a sítios teloméricos intersticiais (ITS) evidenciaram o envolvimento de múltiplos clusters de rDNA 5S em rearranjos cromossômicos nas subfamílias Loricariinae e Ancistrinae. Vestígios de sequências (TTAGGG)n em sítios internos podem corresponder à locais de rearranjos cromossômicos e também são considerados como pontos quentes para quebras cromossômicas, os “sítios frágeis”.
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10

Steitz, J. A., C. Berg, J. P. Hendrick, et al. "A 5S rRNA/L5 complex is a precursor to ribosome assembly in mammalian cells." Journal of Cell Biology 106, no. 3 (1988): 545–56. http://dx.doi.org/10.1083/jcb.106.3.545.

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A novel 5S RNA-protein (RNP) complex in human and mouse cells has been analyzed using patient autoantibodies. The RNP is small (approximately 7S) and contains most of the nonribosome-associated 5S RNA molecules in HeLa cells. The 5S RNA in the particle is matured at its 3' end, consistent with the results of in vivo pulse-chase experiments which indicate that this RNP represents a later step in 5S biogenesis than a previously described 5S*/La protein complex. The protein moiety of the 5S RNP has been identified as ribosomal protein L5, which is known to be released from ribosomes in a complex
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11

Wich, Günter, Lionel Sibold, and August Böck. "Divergent Evolution of 5 S rRNA Genes in Methanococcus." Zeitschrift für Naturforschung C 42, no. 4 (1987): 373–80. http://dx.doi.org/10.1515/znc-1987-0408.

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Abstract The organization of genes for 5S rRNA in the methanogenic archaebacterium Methanococcus (M .) voltae and their nucleotide sequences have been determined. M. voltae possesses three 5S rRNA genes, one of them is organized in an rRNA transcriptional unit coding for 16S-23S-5S rRNA. The other two are associated with seven tRNA genes in a putative transcriptional unit composed of 5′-tRNAThr-tRNAPro-tRNATyr-tRNALys - 5S rRNA-tRNAAsp-tRNALys - 5S rRNA-tRNAAsp-3′. Coding regions plus spacers of the tRNALys-5S rRNA-tRNAAsp block of this gene cluster occur twice with identical sequence. The 5S
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12

Indarti, Caecilia, Endah Wahjuningsih, and Arya Brahmanta. "Analysis Of 5R Implementation at Nala Husada Dental Hospital Surabaya." Interdental Jurnal Kedokteran Gigi (IJKG) 20, no. 3 (2024): 474–82. https://doi.org/10.46862/interdental.v20i3.9660.

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Introduction. The 5S culture (Sort, Set Order, Shine, Standardize, Sustain) is a culture adopted from Japan, a way of building and maintaining a clean, comfortable and set order environment. The implementation of the 5S culture at the Nala Husada Dental Hospital will have an impact on the process of improving safety and service quality which is an important component of service to patients. Initial survey results show that the 5S culture is not yet running, so it is a problem that needs to be solved. Materials and Methods. This type of research is experimental research. The 5S socialization wa
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13

Hidayat, Rizal Taufiq, Dwi Maryono, and Dwi Esti Wardani. "Pengaruh Faktor-Faktor Internal dan Eksternal terhadap Keberhasilan Penerapan Budaya Kerja 5S di SMK." IJIE (Indonesian Journal of Informatics Education) 7, no. 2 (2024): 111. http://dx.doi.org/10.20961/ijie.v7i2.81711.

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<span id="docs-internal-guid-f25bb65c-7fff-3925-fd37-976c4977f61c"><span>Budaya kerja 5S (Seiri, Seiton, Seiso, Seiketsu, dan Shitsuke) merupakan salah satu metode manajemen yang dapat diterapkan untuk meningkatkan efisiensi dan produktivitas kerja. Penerapan budaya kerja 5S di SMK memiliki potensi untuk meningkatkan kualitas lulusan SMK yang siap kerja. Namun, masih ada beberapa gap dalam pemahaman tentang penerapan budaya kerja 5S di SMK, salah satunya adalah kurangnya penelitian tentang faktor-faktor yang mempengaruhi keberhasilan penerapan budaya kerja 5S. Penelitian ini bertuj
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14

Samson, M. L., and M. Wegnez. "Bipartite structure of the 5S ribosomal gene family in a Drosophila melanogaster strain, and its evolutionary implications." Genetics 118, no. 4 (1988): 685–91. http://dx.doi.org/10.1093/genetics/118.4.685.

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Abstract Knowledge of multigenic family organization should provide insight into their mode of evolution. Accordingly, we characterized the 5S ribosomal gene family in the Drosophila melanogaster strain ry506. The 5S genes in this strain display a striking HindIII restriction difference compared to the "standard" D. melanogaster 5S genes. The sequence of three ry506 5S genes was determined. We show that the HindIII restriction site heterogeneity within the ry506 5S family most probably results from the same point mutation, suggesting that a single 5S variant was propagated into the 5S cluster
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15

Wasko, Adriane Pinto, Cesar Martins, Jonathan M. Wright, and Pedro Manoel Galetti Jr. "Molecular organization of 5S rDNA in fishes of the genus Brycon." Genome 44, no. 5 (2001): 893–902. http://dx.doi.org/10.1139/g01-067.

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There are few reports on the genomic organization of 5S rDNA in fish species. To characterize the 5S rDNA nucleotide sequence and chromosomal localization in the Neotropical fishes of the genus Brycon, 5S rDNA copies from seven species were generated by PCR. The nucleotide sequences of the coding region (5S rRNA gene) and the nontranscribed spacer (NTS) were determined, revealing that the 5S rRNA genes were highly conserved, while the NTSs were widely variable among the species analyzed. Moreover, two classes of NTS were detected in each species, characterized by base substitutions and inserti
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16

Choi, Young A., Ryutaro Tao, Keizo Yonemori, and Akira Sugiura. "Simultaneous Visualization of 5S and 45S rDNAs in Persimmon (Diospyros kaki) and Several Wild Relatives (Diospyros spp.) by Fluorescent in situ Hybridization (FISH) and MultiColor FISH (MCFISH)." Journal of the American Society for Horticultural Science 128, no. 5 (2003): 736–40. http://dx.doi.org/10.21273/jashs.128.5.0736.

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5S ribosomal DNA (rDNA) was visualized on the somatic metaphase chromosome of persimmon (Diospyros kaki) and ten wild Diospyros species by fluorescent in situ hybridization (FISH). The digoxigenin (DIG)-labeled 5S rDNA probe was hybridized onto the chromosomes and visualized by incubation with anti-DIG-fluorescein isothiocyanate (FITC). Strong signals of 5S rDNA probe were observed on several chromosomes of Diospyros species tested. Furthermore, multicolor FISH using 5S and 45S rDNA probes differently labeled with DIG and biotin, revealed separate localization of the two rDNA genes on differen
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17

Choi, Young A., Ryutaro Tao, Keizo Yonemori, and Akira Sugiura. "Simultaneous Visualization of 5S and 45S rDNAs in Persimmon (Diospyros kaki) and Several Wild Relatives (Diospyros spp.) by Fluorescent in situ Hybridization (FISH) and MultiColor FISH (MCFISH)." Journal of the American Society for Horticultural Science 128, no. 5 (2003): 736–40. https://doi.org/10.21273/jashs.128.5.736.

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5S ribosomal DNA (rDNA) was visualized on the somatic metaphase chromosome of persimmon (Diospyros kaki) and ten wild Diospyros species by fluorescent in situ hybridization (FISH). The digoxigenin (DIG)-labeled 5S rDNA probe was hybridized onto the chromosomes and visualized by incubation with anti-DIG-fluorescein isothiocyanate (FITC). Strong signals of 5S rDNA probe were observed on several chromosomes of Diospyros species tested. Furthermore, multicolor FISH using 5S and 45S rDNA probes differently labeled with DIG and biotin, revealed separate localization of the two rDNA genes on differen
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18

Peck, L. J., L. Millstein, P. Eversole-Cire, J. M. Gottesfeld, and A. Varshavsky. "Transcriptionally inactive oocyte-type 5S RNA genes of Xenopus laevis are complexed with TFIIIA in vitro." Molecular and Cellular Biology 7, no. 10 (1987): 3503–10. http://dx.doi.org/10.1128/mcb.7.10.3503-3510.1987.

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An extract from whole oocytes of Xenopus laevis was shown to transcribe somatic-type 5S RNA genes approximately 100-fold more efficiently than oocyte-type 5S RNA genes. This preference was at least 10-fold greater than the preference seen upon microinjection of 5S RNA genes into oocyte nuclei or upon in vitro transcription in an oocyte nuclear extract. The approximately 100-fold transcriptional bias in favor of the somatic-type 5S RNA genes observed in vitro in the whole oocyte extract was similar to the transcriptional bias observed in developing Xenopus embryos. We also showed that in the wh
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19

Akram, Muhammad Waseem, Ansar Abbas, Irfan Ahmad Khan, and Muhammad Farooq Ahmad. "The Impact of Effective Implementation of the 5S Concept on Company Performance: A Case Study of a Manufacturing Company." NICE Research Journal 16, no. 2 (2023): 119–40. http://dx.doi.org/10.51239/nrjss.v16i2.428.

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Purpose- This study examines how 5S affects a Pakistani manufacturing company’s operation. The investigation was quantitative. A Pakistani electrical manufacturer received the poll. At least 180 replies were collected from 200 samples. Methodology-This study investigated hypotheses from a conceptual model of the 5S concept and productivity using partial least squares structural equation modelling (PLS-SEM). The statistics showed that 5S improved the organization's productivity. Results-The results also demonstrated that the 5S technique increased role productivity and organization success. Suc
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20

Fukui, K., Y. Kamisugi, and F. Sakai. "Physical mapping of 5S rDNA loci by direct-cloned biotinylated probes in barley chromosomes." Genome 37, no. 1 (1994): 105–11. http://dx.doi.org/10.1139/g94-013.

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5S rDNA loci have been mapped on barley chromosomes by in situ hybridization using five reciprocal translocation lines. Two kinds of DNA probes covering either the 5S rDNA coding region or the 5S rDNA coding and flanking noncoding regions were used. They were prepared by direct cloning from interphase nuclei and simultaneous direct labeling in PCR. Four 5S rDNA loci were detected in a haploid genome by the 5S rDNA coding region, whereas in addition, the four or six 5S rDNA related sites, depending on the variety used, were revealed by the probe covering the flanking region. The four 5S rDNA lo
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21

Peck, L. J., L. Millstein, P. Eversole-Cire, J. M. Gottesfeld, and A. Varshavsky. "Transcriptionally inactive oocyte-type 5S RNA genes of Xenopus laevis are complexed with TFIIIA in vitro." Molecular and Cellular Biology 7, no. 10 (1987): 3503–10. http://dx.doi.org/10.1128/mcb.7.10.3503.

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An extract from whole oocytes of Xenopus laevis was shown to transcribe somatic-type 5S RNA genes approximately 100-fold more efficiently than oocyte-type 5S RNA genes. This preference was at least 10-fold greater than the preference seen upon microinjection of 5S RNA genes into oocyte nuclei or upon in vitro transcription in an oocyte nuclear extract. The approximately 100-fold transcriptional bias in favor of the somatic-type 5S RNA genes observed in vitro in the whole oocyte extract was similar to the transcriptional bias observed in developing Xenopus embryos. We also showed that in the wh
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22

Dyomin, Alexander, Svetlana Galkina, Arina Ilina, and Elena Gaginskaya. "Single Copies of the 5S rRNA Inserted into 45S rDNA Intergenic Spacers in the Genomes of Nototheniidae (Perciformes, Actinopterygii)." International Journal of Molecular Sciences 24, no. 8 (2023): 7376. http://dx.doi.org/10.3390/ijms24087376.

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In the vast majority of Animalia genomes, the 5S rRNA gene repeats are located on chromosomes outside of the 45S rDNA arrays of the nucleolar organiser (NOR). We analysed the genomic databases available and found that a 5S rDNA sequence is inserted into the intergenic spacer (IGS) between the 45S rDNA repeats in ten species of the family Nototheniidae (Perciformes, Actinopterigii). We call this sequence the NOR-5S rRNA gene. Along with Testudines and Crocodilia, this is the second case of a close association between four rRNA genes within one repetitive unit in deuterostomes. In both cases, NO
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23

Khoryanton, Ampala, Sri Harmanto, and Ignatius W. Gunawan. "Assessment Standards for 5S Implementation on SMEs of Ship Component." Journal of Southwest Jiaotong University 56, no. 2 (2021): 32–41. http://dx.doi.org/10.35741/issn.0258-2724.56.2.4.

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This article describes a new idea in applying the 5S concept (Seiri, Seiton, Seiso, Seiketsu, Shitsuke) to create a quality work environment for small and medium-sized enterprises of ship components. The success rate of implementing the 5S concept must be known based on assessing the effectiveness of the 5S concept application. Assessment of the effectiveness of the application of the 5S concept requires measurement indicators that are with the characteristics work environment of small and medium-sized enterprises of ship components. Determination of standard indicators for the implementation
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24

Christian, Rio Surya. "PENERAPAN EVALUASI RINGKAS, RAPI, RESIK, RAWAT, RAJIN PT. INKA (PERSERO) MADIUN." Indonesian Journal of Occupational Safety and Health 7, no. 1 (2018): 11. http://dx.doi.org/10.20473/ijosh.v7i1.2018.11-19.

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Sort, Set, Shine, Standardize, and Sustain (5S) program is an attempt to minimize or eliminate harm by creating a good workplace culture in the workplace. A comfortable working environment will improve efficiency, productivity, quality, and safety. The 5S program is one of the administrative controls used to prevent accidents. 5S implementation will be reached and applied with support from all sides involved, start from top management, middle management, and low management. The study aims to evaluate the application of 5S in PT. INKA (Persero) Madiun. This study was observational and descripti
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25

Drouin, Guy. "Expressed retrotransposed 5S rRNA genes in the mouse and rat genomes." Genome 43, no. 1 (2000): 213–15. http://dx.doi.org/10.1139/g99-100.

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The analyses of previously described 5S rRNA gene sequences show that some of the expressed 5S rRNA genes present in the mouse and rat genomes were derived from the retrotransposition of 5S rRNA transcripts. These analyses demonstrate that new 5S rRNA gene copies can originate by retrotransposition and that some of these retrotranscribed genes are expressed. Key words: 5S ribosomal RNA genes, retrotransposition, retroposons.
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26

Gunawan, Muhkhammat Sahrul. "ANALISIS PENERAPAN 5R PADA PT SUKUN TRANSPORT LOGISTIK." Journal of Industrial Engineering and Technology 1, no. 2 (2021): 27–37. http://dx.doi.org/10.24176/jointtech.v1i2.6495.

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5S yang dikembangkan di jepang, disebut 5S (Seiri, Seiton, Seiso, Seiketsu, dan Shitsuke) yang merupakan sarana mencapai Efisiensi, Produktivitas, Kualitas, dan Keselamatan Kerja. Pengambilan topik 5S pada laporan ini dilakukan berdasar penghayatan bahwa tenaga kerja plus budaya 5S merupakan harta utama bagi perusahaan. Tujuan dari laporan ini adalah untuk mengetahui kondisi area kerja di perusahaan PT Sukun Transport logistik dengan penerapan 5S. Kelebihan dari adanya implementasi tersebut antara lain membantu perusahaan dalam membenahi pola pikir pekerja terhadap pembiasaan 5S di area kerja.
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27

MORENO-CAMPOS, RODRIGO, LUIS E. FLORENCIO-MARTÍNEZ, TOMÁS NEPOMUCENO-MEJÍA, et al. "Molecular characterization of 5S ribosomal RNA genes and transcripts in the protozoan parasite Leishmania major." Parasitology 143, no. 14 (2016): 1917–29. http://dx.doi.org/10.1017/s0031182016001712.

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SUMMARYEukaryotic 5S rRNA, synthesized by RNA polymerase III (Pol III), is an essential component of the large ribosomal subunit. Most organisms contain hundreds of 5S rRNA genes organized into tandem arrays. However, the genome of the protozoan parasite Leishmania major contains only 11 copies of the 5S rRNA gene, which are interspersed and associated with other Pol III-transcribed genes. Here we report that, in general, the number and order of the 5S rRNA genes is conserved between different species of Leishmania. While in most organisms 5S rRNA genes are normally associated with the nucleol
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28

Gottlob-McHugh, S. G., M. Lévesque, K. MacKenzie, M. Olson, O. Yarosh, and D. A. Johnson. "Organization of the 5S rRNA genes in the soybean Glycine max (L.) Merrill and conservation of the 5S rDNA repeat structure in higher plants." Genome 33, no. 4 (1990): 486–94. http://dx.doi.org/10.1139/g90-072.

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The 5S rRNA gene of the soybean Glycine max (L.) Merr. has been cloned on a 556-bp fragment of DNA and sequenced. This fragment contains two copies of the soybean 5S rDNA sequence, one intact and one truncated, separated by noncoding DNA. We have used this clone to investigate the organization of the 5S genes within the soybean genome and the extent of their methylation. Our results demonstrate that soybean 5S genes are clustered, organized into tandem repeats of 330 bp, and extensively methylated. Hybridization of the 5S sequence to Southern transfers of soybean DNA digested with BamHI reveal
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29

Stratiichuk, A. S., T. O. Derevenko, and Y. O. Tynkevych. "Organization of 5S rDNA repeated unit of Quercus imbricaria Michx." Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv 17, no. 2 (2020): 179–86. http://dx.doi.org/10.7124/visnyk.utgis.17.2.1219.

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Aim. The 5S rDNA repeats represent a universal model for the investigation of molecular evolution of repeated sequences. Also, comparison of 5S rDNA was successfully applied for the elucidation of phylogenetic relationships between the closely related plant species. However, there is practically no data regarding the molecular organization of 5S rDNA repeats in members of the section Lobatae, one of the largest groups of the genus Quercus. Accordingly, our aim was to investigate the 5S rDNA organization for Q. imbricaria, a species that belongs to this section. Methods. DNA extraction, PCR amp
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30

Gupta, Shaman, and Sanjiv Kumar Jain. "An application of 5S concept to organize the workplace at a scientific instruments manufacturing company." International Journal of Lean Six Sigma 6, no. 1 (2015): 73–88. http://dx.doi.org/10.1108/ijlss-08-2013-0047.

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Purpose – The purpose of this paper is to use the 5S tool to assist a small-scale manufacturing organization to become more productive and more efficient. Design/methodology/approach – A simple approach has been adopted to create the teams for implementing 5S. Cause-and-effect diagram has been studied for shop floor analysis. Later, four data collection methods have been used to ensure right implementation of the 5S. Findings – In the frames of this case study, it has been analyzed that implementation of “5S” resulted in overall improvement of the organization. With the implementation of “5S”,
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Rusmiati, Emi, Laksmi Ambarwati, and Dede Santoni. "Edukasi 5S dalam Upaya Continuous Improvement Melalui Audit 5S Pada PT Inti Ganda Perdana (IGP)." Journal of Community Services in Sustainability 1, no. 1 (2023): 9–18. http://dx.doi.org/10.52330/jocss.v1i1.144.

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The good industrial development in Indonesia gave rise to new industries aimed at increasing production capacity. Efforts are made to increase capacity by carrying out continuous improvement. One that can be done is to provide an understanding of 5S and 5S audits. PT IGP has so far implemented 5S, but sometimes employees still need to be reminded of the 5S implementation. Therefore it is necessary to carry out 5S audits and internalize 5S understanding for employees and management. This Community Service Activity (PkM) was carried out. Beginning with observation, problem identification, litera
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32

Ishchenko, O. O., V. V. Kozub, and I. I. Panchuk. "Organization of 5S ribosomal DNA of Litchi chinensis Sonn." Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv 18, no. 1-2 (2021): 3–8. http://dx.doi.org/10.7124/visnyk.utgis.18.1-2.1348.

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Aim. 5S ribosomal DNA (5S rDNA) represents a universal model for studying the evolution of repeated sequences in eukaryotic organisms. Taking into account that this region of the genome still remains almost undescribed in species of the family Sapindaceae, we investigated the molecular organization of a repeated unit of 5S rDNA in a member of this family, Litchi chinensis. Methods. PCR amplification, cloning and sequencing of 5S rDNA. Results. It was found that the length of the repeated unit of the 5S rDNA of L. chinensis is 321 -323 bp. The level of intragenomic similarity of 5S rDNA repeats
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33

Darby, M. K., and K. E. Joho. "Differential binding of zinc fingers from Xenopus TFIIIA and p43 to 5S RNA and the 5S RNA gene." Molecular and Cellular Biology 12, no. 7 (1992): 3155–64. http://dx.doi.org/10.1128/mcb.12.7.3155-3164.1992.

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Zinc fingers are usually associated with proteins that interact with DNA. Yet in two oocyte-specific Xenopus proteins, TFIIA and p43, zinc fingers are used to bind 5S RNA. One of these, TFIIIA, also binds the 5S RNA gene. Both proteins have nine zinc fingers that are nearly identical with respect to size and spacing. We have determined the relative affinities of groups of zinc fingers from TFIIIA for both 5S RNA and the 5S RNA gene. We have also determined the relative affinities of groups of zinc fingers from p43 for 5S RNA. The primary protein regions for RNA and DNA interaction in TFIIIA ar
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34

Darby, M. K., and K. E. Joho. "Differential binding of zinc fingers from Xenopus TFIIIA and p43 to 5S RNA and the 5S RNA gene." Molecular and Cellular Biology 12, no. 7 (1992): 3155–64. http://dx.doi.org/10.1128/mcb.12.7.3155.

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Zinc fingers are usually associated with proteins that interact with DNA. Yet in two oocyte-specific Xenopus proteins, TFIIA and p43, zinc fingers are used to bind 5S RNA. One of these, TFIIIA, also binds the 5S RNA gene. Both proteins have nine zinc fingers that are nearly identical with respect to size and spacing. We have determined the relative affinities of groups of zinc fingers from TFIIIA for both 5S RNA and the 5S RNA gene. We have also determined the relative affinities of groups of zinc fingers from p43 for 5S RNA. The primary protein regions for RNA and DNA interaction in TFIIIA ar
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35

Bogdanov, Alexey A., Olga A. Dontsova, Svetlana S. Dokudovskaya, and Inna N. Lavrik. "Structure and function of 5S rRNA in the ribosome." Biochemistry and Cell Biology 73, no. 11-12 (1995): 869–76. http://dx.doi.org/10.1139/o95-094.

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5S rRNA is a small RNA molecule that is a component of a ribosome from almost all living organisms. In this review, we discuss the biogenesis of 5S rRNA and its properties as an independent structural domain of a ribosome as well as the current concepts concerning the higher order structure of 5S rRNA in free state and in its complexes with ribosomal proteins and its folding in the ribosome. Special attention is paid to recent experimental approaches that have been useful in 5S rRNA studies. Our own data on topography of 5S rRNA in the ribosomes are discussed in detail. The hypothesis describi
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36

Chandra, Huki, Yashmine Mela Ardianto, Norma Novika, Febrin Claudia Angeli, and Nilna Agnia. "Implementation of 5S and Safety Awareness in the Classroom Environment at Telkom University Surabaya." IJIEM - Indonesian Journal of Industrial Engineering and Management 5, no. 1 (2024): 107. http://dx.doi.org/10.22441/ijiem.v5i1.22804.

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The application of 5S culture is now an obligation that must be implemented by every place for the convenience of users or building occupants. The implementation of 5S that has been designed can produce an effective, efficient, productive work culture and uphold work safety. 5S culture is a culture of one of the concepts of industrial engineering. This concept is simple, easy to understand, and is the first step in the dissemination of industrial engineering culture. 5S itself is not only applied to office buildings but can also be applied to classes where various activities are carried out an
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37

Unadiroh, Lulu', Wawan Shokib Rondli, and M. Syafruddin Kuryanto. "Analisis Pelaksanaan Budaya 5s Untuk Menguatkan Karakter Peduli Sosial Di SDN Tambirejo." Jurnal Citizenship Virtues 5, no. 1 (2025): 70–77. https://doi.org/10.37640/jcv.v5i1.2311.

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Penelitian ini dilaksanakan karena banyaknya siswa yang belum peduli terhadap sekitar. Oleh karena itu perlu diadakannya penguatan karakter peduli sosial melalui budaya 5S di SDN Tambirejo. Dalam membentuk karakter peduli sosial pada siswa menjadi lebih baik, guru SDN Tambirejo melaksanakan budaya 5S dan memberikan contoh bagi siswa. Penelitian ini bertujuan untuk mengetahui pelaksanakan budaya 5S (Senyum, Sapa, Salam, Sopan, Santun) pada siswa SDN Tambirejo, dengan rumusan masalah mendeskripsikan pelaksanaan budaya 5S pada siswa SD dan menganalisis faktor pendukung dan penghambat dalam pelaks
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38

Mutiara, Amaliyah, and Siti Aisyah. "Implementasi Pengarsipan Dokumen Menggunakan Metode 5S di Kantor Dinas Perikanan Lubuk Pakam." Jurnal Minfo Polgan 12, no. 2 (2023): 2146–50. http://dx.doi.org/10.33395/jmp.v12i2.13179.

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Dokumen-dokumen yang berkaitan dengan departemen ini disimpan di gudang kantor dinas perikanan Lubuk Pakam kabupaten Deli Serdang. Akibat belum lengkapnya penerapan 5S Seiri (Sort), Seiton (Halus), Seiso (Polandia), Seiketsu (Standarisasi), dan Shitsuke (Maintain), maka gudang penyimpanan catatan dokumen digudang mengalami kendala. Tujuan dari penelitian ini adalah menerapkan 5S pada penyimpanan arsip dokumen di kantor dinas agar mendapatkan hasil yang lebih baik. Metode yang digunakan adalah metode 5S dengan menggunakan kuesioner dalam mengidentifikasi, menganalisa serta mengetahui dampak pen
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39

Wang, Lei, Martin Ciganda, and Noreen Williams. "Defining the RNA-Protein Interactions in the Trypanosome Preribosomal Complex." Eukaryotic Cell 12, no. 4 (2013): 559–66. http://dx.doi.org/10.1128/ec.00004-13.

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ABSTRACT In eukaryotes, 5S rRNA is transcribed in the nucleoplasm and requires the ribosomal protein L5 to deliver it to the nucleolus for ribosomal assembly. The trypanosome-specific proteins P34 and P37 form a novel preribosomal complex with the eukaryotic conserved L5-5S rRNA complex in the nucleoplasm. Previous results suggested that P34 acts together with L5 to bridge the interaction with 5S rRNA and thus to stabilize 5S rRNA, an important role in the early steps of ribosomal biogenesis. Here, we have delineated the domains of the two protein components, L5 and P34, and regions of the RNA
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40

Volarić, Marin, Evelin Despot-Slade, Damira Veseljak, et al. "The Genome Organization of 5S rRNA Genes in the Model Organism Tribolium castaneum and Its Sibling Species Tribolium freemani." Genes 15, no. 6 (2024): 776. http://dx.doi.org/10.3390/genes15060776.

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5S ribosomal DNAs (rDNAs) are arranged in tandem and are often under-represented in genome assemblies. In the present study, we performed a global and in-depth analysis of the 5S rDNAs in the model insect Tribolium castaneum and its closely related species Tribolium freemani. To accomplish this goal, we used our recently published genome assemblies based on Nanopore and PacBio long-read sequencing. Although these closely related species share the 5S rRNA gene sequence with high homology, they show a different organization of the 5S rDNA locus. Analysis of 5S rDNA arrays in T. castaneum reveale
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41

Qowim, Miftahul, Nina Aini Mahbubah, and M. Zainuddin Fathoni. "PENERAPAN 5S PADA DIVISI GUDANG (STUDI KASUS PT. SUMBER URIP SEJATI)." JUSTI (Jurnal Sistem dan Teknik Industri) 1, no. 1 (2020): 49. http://dx.doi.org/10.30587/justicb.v1i1.2032.

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Dalam meningkatkan mutu, selalu ada banyak cara dan alat yang dapat digunakan, mutu untuk lingkungan tempat kerja 5S (Seiri, Seiton, Seiso, Seketsu, Shitsuke) merupakan salah salah satu metode yang telah efektif dalam manajemen penataan gudang. PT. sumber urip sejati telah mengimplementasikan pendekatan 5S pada seluruh devisi. Namun pada devisi gudang tiga tidak dilakukan evaluasi dan assessment 5S. untuk mengetahui hasil implementasi 5S, maka dilakukan evaluasi dan assesment 5S (Seiri, Seiton, Seiso, Seketsu, Shitsuke) pada divisi gudang tiga. selain hal tersebut peneliti juga memberikan usul
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42

Dilargo, M. Iqbal, Adiguna Sasama Wahyu Utama, and Hindra Kurniawan. "Peningkatan Efisiensi Ruang Kantor Melalui Metode 5S: Analisis Kualitatif Ruang Kantor Unit Taman Rekreasi PT. Selecta." PANDITA : Interdisciplinary Journal of Public Affairs 6, no. 2 (2023): 121–29. http://dx.doi.org/10.61332/ijpa.v6i2.70.

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Penelitian ini bertujuan untuk mengevaluasi sejauh mana penerapan metode 5S di PT. Selecta. Penelitian ini menggunakan pendekatan kualitatif deskriptif untuk menganalisis ruang kantor. Hasil penelitian menunjukkan bahwa penerapan metode 5S belum ditemukan dalam penataan ruang kantor di PT. Selecta. Ketidakteraturan dan kekacauan lingkungan kantor menjadi indikasi ketiadaan praktik 5S. Kesimpulan penelitian ini adalah PT. Selecta belum menerapkan metode 5S dalam penataan ruang kantornya. Temuan ini menunjukkan perlunya PT. Selecta untuk mempertimbangkan penerapan metode 5S guna meningkatkan efi
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43

Nguyễn, Quỳnh Trúc. "KIẾN THỨC VỀ 5S VÀ MỘT SỐ YẾU TỐ LIÊN QUAN ĐẾN KIẾN THỨC 5S CỦA NHÂN VIÊN Y TẾ TẠI MỘT SỐ BỆNH VIỆN TUYẾN HUYỆN, THÀNH PHỐ CẦN THƠ, NĂM 2022". Tạp chí Y Dược học Cần Thơ, № 50 (20 серпня 2022): 150–56. http://dx.doi.org/10.58490/ctump.2022i50.137.

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Đặt vấn đề: Sàng lọc, Sắp xếp, Sạch sẽ, Săn sóc, Sẵn sàng (5S) là nền tảng của phương pháp chăm sóc sức khỏe tinh gọn. Hiện nay, 5S được áp dụng cho lĩnh vực chăm sóc sức khỏe như một phương pháp tổ chức và chuẩn hóa tại Bệnh viện. Mục tiêu nghiên cứu: Xác định tỷ lệ đáp ứng 5S và phân tích một số yếu tố liên quan đến kiến thức 5S của nhân viên y tế (NVYT) tại Bệnh viện tuyến huyện thuộc thành phố Cần Thơ. Đối tượng và phương pháp nghiên cứu: Nghiên cứu mô tả cắt ngang trên 164 NVYT hiện đang làm việc tại hai bệnh viện tuyến huyện, thành phố Cần Thơ năm năm 2020. Các khảo sát được thực hiện tr
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44

Lysenko, Oleksandr, Dmytro Lebedev, Volodymyr Davydenko, and Anatoliy Miroshnychenko. "Secrets of successful implementation of 5S." Electronic Scientific Journal Intellectualization of Logistics and Supply Chain Management #1 2020, no. 14 (October 2022): 31–39. http://dx.doi.org/10.46783/smart-scm/2022-14-3.

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The article is devoted to the analysis of the possibilities of implementing 5S at the organization. The component of the 5S system is considered. The article describes the theoretical and practical aspects of the transition to the 5S system. Classical approaches are highlighted, the main reasons that lead to the occurrence of resource losses are considered. An analysis of the basic tools and methods that can be applied during the implementation of the transition to the 5S system was carried out. The possibilities of obtaining benefits from the introduction of the 5S system are considered. Appr
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45

Martins, Cesar, and Pedro Manoel Galetti Jr. "Organization of 5S rDNA in species of the fish Leporinus: two different genomic locations are characterized by distinct nontranscribed spacers." Genome 44, no. 5 (2001): 903–10. http://dx.doi.org/10.1139/g01-069.

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To address understanding the organization of the 5S rRNA multigene family in the fish genome, the nucleotide sequence and organization array of 5S rDNA were investigated in the genus Leporinus, a representative freshwater fish group of South American fauna. PCR, subgenomic library screening, genomic blotting, fluorescence in situ hybridization, and DNA sequencing were employed in this study. Two arrays of 5S rDNA were identified for all species investigated, one consisting of monomeric repeat units of around 200 bp and another one with monomers of 900 bp. These 5S rDNA arrays were characterize
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46

Faradilla, Arnes, Winnie Septiani, Nora Azmi, and Syafa Kansa. "PERANCANGAN RUANG KERJA DOSEN DAN PENATAAN DOKUMEN MENGGUNAKAN METODE 5S DI JURUSAN TEKNIK INDUSTRI, UNIVERSITAS TRISAKTI." J@ti Undip : Jurnal Teknik Industri 14, no. 2 (2019): 81. http://dx.doi.org/10.14710/jati.14.2.81-86.

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Setiap Dosen memiliki tugas utama terkait dengan tridharma yaitu Pendidikan dan Pengajaran, Penelitian, Pengabdian Kepada Masyarakat (PKM), dan Penunjang Tridharma. Dokumen yang digunakan banyak dan harus disimpan dengan rapi di ruang kerja untuk keperluan kegiatan Dosen seperti Beban Kerja Dosen (BKD) dan kepangkatan. Hasil penelitian pendahuluan menunjukkan sebanyak 62,5% Dosen menyatakan kesulitan dalam menyimpan dokumen di ruang kerja dan sebanyak 81,25% menyatakan perlu adanya perbaikan di ruang kerja. Penelitian ini bertujuan untuk merancang ruang kerja Dosen dan penataan dokumen menggun
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47

Oktafiani, Ilmiah Sholikhah, and Subiyantoro Subiyantoro. "Application of 5S Management in Improving the Quality of Furniture Production in Meubel Karya Agung of Ponorogo Regency." PALAPA 10, no. 1 (2022): 45–60. http://dx.doi.org/10.36088/palapa.v10i1.1658.

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5S (Sort, Set in order, Shine, Standardize, dan Self Discipline) is a work culture that must be applied in all workplaces both in the formal and informal sectors. The implementation of 5S aims to make workers feel comfortable and safe while working. In addition, the application of 5S can make work more effective and efficient because it can eliminate wasted time due to more time to search for goods, work areas that look full due to excess equipment and un needed goods or dirty environments. Furniture Works In Semanding District, Kauman, Ponorogo is one of the informal sectors that have not imp
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48

Guinta, D. R., and L. J. Korn. "Differential order of replication of Xenopus laevis 5S RNA genes." Molecular and Cellular Biology 6, no. 7 (1986): 2536–42. http://dx.doi.org/10.1128/mcb.6.7.2536-2542.1986.

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In Xenopus laevis there are two multigene families of 5S RNA genes: the oocyte-type 5S RNA genes which are expressed only in oocytes and the somatic-type 5S RNA genes which are expressed throughout development. The Xenopus 5S RNA replication-expression model of Gottesfeld and Bloomer (Cell 28:781-791, 1982) and Wormington et al. (Cold Spring Harbor Symp. Quant. Biol. 47:879-884, 1983) predicts that the somatic-type 5S RNA genes replicate earlier in the cell cycle than do the oocyte-type genes. Hence, the somatic-type 5S RNA genes have a competitive advantage in binding the transcription factor
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49

Srinivasan, Siddarth, Laura Hughes Ikuma, Mahmoud Shakouri, Isabelina Nahmens, and Craig Harvey. "5S impact on safety climate of manufacturing workers." Journal of Manufacturing Technology Management 27, no. 3 (2016): 364–78. http://dx.doi.org/10.1108/jmtm-07-2015-0053.

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Purpose – 5S is a commonly used Lean tool that focusses on creating an organized work environment, but the effects of 5S on safety climate are not as well studied. The purpose of this paper is to determine the impact of a 5S event on safety climate. Design/methodology/approach – This pre-test post-test study examines the effect of implementing 5S on safety climate of the packaging area of a manufacturing plant. Two groups of employees (case and control groups) completed a safety climate questionnaire (Safety Climate Assessment Toolkit) prior to the 5S event, one month after, and two months aft
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50

Guinta, D. R., and L. J. Korn. "Differential order of replication of Xenopus laevis 5S RNA genes." Molecular and Cellular Biology 6, no. 7 (1986): 2536–42. http://dx.doi.org/10.1128/mcb.6.7.2536.

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In Xenopus laevis there are two multigene families of 5S RNA genes: the oocyte-type 5S RNA genes which are expressed only in oocytes and the somatic-type 5S RNA genes which are expressed throughout development. The Xenopus 5S RNA replication-expression model of Gottesfeld and Bloomer (Cell 28:781-791, 1982) and Wormington et al. (Cold Spring Harbor Symp. Quant. Biol. 47:879-884, 1983) predicts that the somatic-type 5S RNA genes replicate earlier in the cell cycle than do the oocyte-type genes. Hence, the somatic-type 5S RNA genes have a competitive advantage in binding the transcription factor
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