Bibliografías temáticas / ADN recombiné – Embryons

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Literatura académica sobre el tema "ADN recombiné – Embryons"

Autor: Grafiati
Publicado: 21 de diciembre de 2024
Última modificación: 31 de julio de 2025

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Artículos de revistas sobre el tema "ADN recombiné – Embryons"

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Liu, J., C. Long, M. Westhusin, and D. Kraemer. "44 ATTEMPTS TO USE SOMATIC CELLS ISOLATED FROM FROZEN BOVINE SEMEN FOR NUCLEAR TRANSFER." Reproduction, Fertility and Development 21, no. 1 (2009): 122. http://dx.doi.org/10.1071/rdv21n1ab44.

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Somatic cells in semen are a potential source of nuclei for nuclear transfer to produce genetically identical animals. This is especially important when an animal has died and the only viable genetic material available is frozen semen. In our previous studies, epithelial cells were cultured from fresh ovine and bovine ejaculates and blastocyst stage embryos were produced using these cells (Liu J et al. 2007 Biol. Reprod. special issue, 177; Liu J et al. 2008 Reprod. Fertil. Dev. 20, 102). However, growing cells from frozen semen can be difficult. We hypothesized that nuclei of the somatic cell
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2

Gong-Jin, Wang, Tan Xiao-Dong, Zhou Xiao-Long, Xu Xiao-Bo, and Fan Bi-Qin. "In vitro fertilization and cleavage of mouse oocytes recombined with the first polar body." Chinese Journal of Agricultural Biotechnology 5, no. 2 (2008): 169–73. http://dx.doi.org/10.1017/s1479236208002143.

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AbstractThe developmental functions of oocytes of three strains of mice (Kunming, ICR and C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb) recombined with the nuclei of first polar bodies (Pbs I) were explored. Cumulus oocyte complexes (COCs) from the mice were collected after superovulation, then Pbs I were obtained from the COCs by 2% pronase treatment. The survival of Pbs I under different temperatures was identified by morphology and trypan blue staining. Later, the polar body I (Pb 1) nucleus and a little cytoplasm was injected into each oocyte, the nuclei of which had been enucleated by micromanipu
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Tominaga, Kentaro, Dan Kechele, Guillermo Sanchez, et al. "GENERATION OF HUMAN INTESTINAL ORGANOIDS CONTAINING TISSUE-RESIDENT IMMUNE CELLS." Inflammatory Bowel Diseases 28, Supplement_1 (2022): S57. http://dx.doi.org/10.1093/ibd/izac015.090.

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Abstract BACKGROUND Human gastrointestinal (GI) organoid technologies have transformed GI disease research. By recapitulating the essential steps that occur during embryonic organ development, we could generate in vitro human colonic organoids (HCOs) (Munera et al, Cell Stem Cell. 2017) and human intestinal organoids (HIOs) (Spence et al, Nature. 2011) from iPSCs. Interestingly, HCOs contain both epithelial and surrounding mesenchymal derivatives, including myofibroblasts and tissue-resident immune cells. Our preliminary data demonstrate that HCOs co-develop CD163 positive macrophages derived
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Minokawa, Takuya, and Shonan Amemiya. "Mesodermal Cell Differentiation in Echinoid Embryos Derived from the Animal Cap Recombined with a Quartet of Micromeres." Zoological Science 15, no. 4 (1998): 541–45. http://dx.doi.org/10.2108/0289-0003(1998)15[541:mcdiee]2.0.co;2.

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Minokawa, Takuya, and Shonan Amemiya. "Mesodermal Cell Differentiation in Echinoid Embryos Derived from the Animal Cap Recombined with a Quartet of Micromeres." ZOOLOGICAL SCIENCE 15, no. 4 (1998): 541–45. http://dx.doi.org/10.2108/zsj.15.541.

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Toren, Eliana, Yanping Liu, and Chad Hunter. "The SSBP3 Co-Regulator Is a Novel Driver of Islet Cell Structure and Function." Journal of the Endocrine Society 5, Supplement_1 (2021): A327. http://dx.doi.org/10.1210/jendso/bvab048.667.

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Abstract The activities of transcriptional complexes drive the proper development and function of insulin producing beta-cells, ultimately required for the regulation of glucose homeostasis. Our prior work helped to establish that the LIM-homeodomain transcription factor (TF), Islet-1 (Isl1), directly interacts with the Ldb1 co-regulator in developing and adult beta-cells. We further found that a member of the Single Stranded DNA-Binding Protein (SSBP) co-regulator family, SSBP3, interacts with the Isl1:Ldb1 complex in beta-cells and primary islets to impact critical target genes MafA and Glp1
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7

Martinez, Julien, Lisa Klasson, John J. Welch, and Francis M. Jiggins. "Life and Death of Selfish Genes: Comparative Genomics Reveals the Dynamic Evolution of Cytoplasmic Incompatibility." Molecular Biology and Evolution 38, no. 1 (2020): 2–15. http://dx.doi.org/10.1093/molbev/msaa209.

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Abstract Cytoplasmic incompatibility is a selfish reproductive manipulation induced by the endosymbiont Wolbachia in arthropods. In males Wolbachia modifies sperm, leading to embryonic mortality in crosses with Wolbachia-free females. In females, Wolbachia rescues the cross and allows development to proceed normally. This provides a reproductive advantage to infected females, allowing the maternally transmitted symbiont to spread rapidly through host populations. We identified homologs of the genes underlying this phenotype, cifA and cifB, in 52 of 71 new and published Wolbachia genome sequenc
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Nakayama, Takuya, Amanda Cox, Mary Howell, and Robert M. Grainger. "Gynogenetic Production of Embryos inXenopus tropicalisUsing a Cold Shock Procedure: Rapid Screening Method for Gene Editing Phenotypes." Cold Spring Harbor Protocols 2022, no. 12 (2022): pdb.prot107648. http://dx.doi.org/10.1101/pdb.prot107648.

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Gynogenesis is a form of parthenogenesis in which eggs require sperm for fertilization but develop to adulthood without the contribution of paternal genome information, which happens naturally in some species. InXenopus, gynogenetic diploid animals can be made experimentally. In mutagenesis strategies that only generate one allele of a recessive mutation, as might occur during gene editing, gynogenesis can be used to quickly reveal a recessive phenotype in eggs carrying a recessive mutation, thereby skipping one generation normally required to screen by conventional genetics.Xenopusoocytes do
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Ye, L., R. Mayberry, E. Stanley, A. Elefanty, and C. Gargett. "134. DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS TO MULLERIAN TISSUE." Reproduction, Fertility and Development 22, no. 9 (2010): 52. http://dx.doi.org/10.1071/srb10abs134.

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The human uterus develops from the distal Mullerian Duct, a derivative of the mesoderm germ layer. Unlike other mammalian species (eg. mouse) the endometrium of the human uterus develops prenatally during gestation. Little is known about the developmental process involved. A better understanding of human endometrial development may shed light on the mechanisms involved in endometrial regeneration and pathogenesis of adult proliferative endometrial diseases. Mouse neonatal uterine mesenchyme (mNUM) is inductive and can maintain the phenotype of normal adult human endometrial epithelial cells [1
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Álvarez-Aznar, A., I. Martínez-Corral, N. Daubel, C. Betsholtz, T. Mäkinen, and K. Gaengel. "Tamoxifen-independent recombination of reporter genes limits lineage tracing and mosaic analysis using CreERT2 lines." Transgenic Research 29, no. 1 (2019): 53–68. http://dx.doi.org/10.1007/s11248-019-00177-8.

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Abstract The CreERT2/loxP system is widely used to induce conditional gene deletion in mice. One of the main advantages of the system is that Cre-mediated recombination can be controlled in time through Tamoxifen administration. This has allowed researchers to study the function of embryonic lethal genes at later developmental timepoints. In addition, CreERT2 mouse lines are commonly used in combination with reporter genes for lineage tracing and mosaic analysis. In order for these experiments to be reliable, it is crucial that the cell labeling approach only marks the desired cell population
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Tesis sobre el tema "ADN recombiné – Embryons"

1

Pijoff, Yannicke. "Colonisation embryonnaire et compétence chimérique des cellules souches pluripotentes : étude chez la souris, le lapin et le chimpanzé." Electronic Thesis or Diss., Lyon 1, 2024. http://www.theses.fr/2024LYO10255.

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Les cellules souches pluripotentes (PSC) naïves ont la capacité de réintégrer le développement embryonnaire normal et de produire des fœtus chimériques chez les rongeurs. Cependant, les PSC naïves provenant d'espèces autres que les rongeurs présentent une capacité nettement moins efficace à coloniser les embryons. Actuellement, notre compréhension des mécanismes impliqués dans la formation des chimères est limitée. Le projet avait pour objectif de mieux comprendre les mécanismes impliqués dans la capacité des PSC à coloniser l’embryon pré-implantatoire. Dans un premier temps, nous nous sommes
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