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Tesis sobre el tema "Affinity labeling"

Autor: Grafiati

Publicado: 4 de junio de 2021

Última modificación: 25 de febrero de 2023

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1

Kuzmich, Oleksandra. "Metal Labeling for Low Affinity Binding Biomolecules." Doctoral thesis, Humboldt-Universität zu Berlin, 2018. http://dx.doi.org/10.18452/18862.

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Unter den Techniken der chemischen Proteomik hat Capture Compound – Massenspektrometrie (CCMS) den Vorteil, Interaktionen von Molekülen mit geringer Affinität zueinander effektiv untersuchen zu können. CCMS beruht auf kleinen molekularen Sonden (Capture Compounds, CCs), die aus drei funktionalen Bestandteilen bestehen: die Selektivitätsfunktion, ist ein kleines Molekül, das mit einem Zielprotein eine schwache Wechselwirkung eingeht. Die zweite Funktionalität erlaubt kovalente Anhaftung der molekularen Sonde an Proteine. Der dritte Anteil erlaubt Detektion mit sehr guten Sensitivität; allerding

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2

Attiya, Said. "Antibody labeling methods for automated affinity electrophoresis on microchips." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0010/NQ59926.pdf.

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3

Seebregts, Christopher J. "Photoaffinity labeling the nucleotide sites of the sarcoplasmic reticulum Ca²⁺-ATPase." Doctoral thesis, University of Cape Town, 1989. http://hdl.handle.net/11427/27167.

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We have synthesized a new class of photoaffinity analogs, 2',3'-O-(2,4,6-trinitrophenyl)-8-azido-ATP, -ADP and -AMP (TNP- 8N₃ATP, -ADP and -AMP), and their radiolabeled derivatives, and characterized their interaction with the sarcoplasmic reticulum Ca²⁺-ATPase. The TNP-8N₃-nucleotides were synthesized from ATP in three steps involving bromination in the 8-position of the adenine ring followed by displacement with an azido group and then trinitrophenylation of the resulting 8N₃-nucleotide with TNBS. Inclusion of the oxidizing agent, DTNB, in the final reaction was found to be necessary to prev

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4

Perols, Anna. "Site-specific labeling of affinity molecules for in vitro and in vivo studies." Doctoral thesis, KTH, Proteinteknologi, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-152349.

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The thesis is focused on site-specific labeling of affinity molecules for different applications where two types of binding proteins, Affibody molecules and antibodies, have been used. For the purpose of improving the properties of Affibody molecules for in vivo imaging, novel bi-functional chelators for radiolabeling using the radionuclide 111In were evaluated. In a first study, two chelators denoted NOTA and DOTA, respectively, were separately conjugated via maleimide chemistry to a C-terminal cysteine residue in a HER2-binding Affibody molecule (ZHER2:2395). In vivo evaluation using mice wi

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5

Lui, James Kwok Ching. "A fluorescent labelling technique to detect changes in the thiol redox state of proteins following mild oxidative stress." University of Western Australia. School of Biomedical, Biomolecular and Chemical Sciences, 2008. http://theses.library.uwa.edu.au/adt-WU2008.0056.

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There is increasing evidence that hydrogen peroxide (H2O2) can act as a signalling molecule capable of modulating a variety of biochemical and genetic systems. Using Jurkat T-lymphocytes, this study initially investigated the involvement of H2O2 in the activation of a specific signalling protein extracellular signal-regulated protein kinase (ERK). It was found that as a result of H2O2 treatment, mitochondrial complex activities decreased which led to subsequent increase of mitochondrial reactive oxygen species (ROS) production. The increase of ROS resulted in higher cellular H2O2 as well as i

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6

Tran, Hang T. "Photocleavable Linker for Protein Affinity Labeling to Identify the Binding Target of KCN-1." Digital Archive @ GSU, 2010. http://digitalarchive.gsu.edu/chemistry_theses/35.

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KCN-1 is known to reduce tumor growth 6-fold in mice implanted with LN229 glioma cells. Although this inhibitor is effective, the mechanism of action for KCN-1 is not well understood. Based on preliminary studies, KCN-1 reduces tumor growth by disrupting the HIF 1 (hypoxia-induced factor-1) pathway. The binding target of KCN-1 needs to be investigated in order to develop KCN-1 or its analogs for therapeutic applications. In this research, a molecule was designed and synthesized for the identification of the binding target of KCN-1. Specifically, this molecule contains the inhibitor (KCN-1

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7

Song, Zhi-Ning. "Development of novel affinity-guided catalysts for specific labeling of endogenous proteins in living systems." Kyoto University, 2017. http://hdl.handle.net/2433/228238.

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8

Kuzmich, Oleksandra [Verfasser], Michael [Gutachter] Linscheid, Hubert [Gutachter] Köster, and Michael [Gutachter] Weller. "Metal Labeling for Low Affinity Binding Biomolecules / Oleksandra Kuzmich ; Gutachter: Michael Linscheid, Hubert Köster, Michael Weller." Berlin : Humboldt-Universität zu Berlin, 2018. http://d-nb.info/1185579265/34.

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9

Bagchi, Pritha. "Expanding the metallomics toolbox: Development of chemical and biological methods in understanding copper biochemistry." Diss., Georgia Institute of Technology, 2013. http://hdl.handle.net/1853/52160.

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Copper is an essential trace element and required for various biological processes, but free copper is toxic. Therefore, copper is tightly regulated in living cells and disruptions in this homeostatic machinery are implicated in numerous diseases. The current understanding of copper homeostasis is substantial but incomplete, particularly in regard to storage and exchange at the subcellular level. Intracellular copper is primarily present in the monovalent oxidation state. Therefore, copper(I) selective fluorescent probes can be utilized for imaging exchangeable copper ions in live cells, but t

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10

Barnett, Derek W. "PART 1. SYNTHESIS OF STABLE-ISOTOPE LABELED AMINO ACIDS PART 2. SYNTHESIS OF MECHANISTIC PROBES OF RETINOID ACTION." The Ohio State University, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=osu1038951598.

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11

Ciccotosto, Silvana. "The preparation and evaluation of N-acetylneuraminic acid derivatives as probes of sialic acid-recognizing proteins." Monash University, Dept. of Medicinal Chemistry, 2004. http://arrow.monash.edu.au/hdl/1959.1/9649.

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12

Brown, Robert Gareth Sumser. "The affinity labelling of gibberellin hydroxylases." Thesis, University of Bristol, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.295169.

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13

Gupta, Neetu. "Inhibitors of intracellular trafficking active against plant and bacterial toxins." Thesis, Paris 11, 2014. http://www.theses.fr/2014PA112328.

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Les toxines Shiga (Stx) sont produites par Shigella dysenteriae et certaines espèces d’E. coli transmisent aux humains par la consommation d'aliments contaminés et causant des maladies graves. La toxine Stx est libérée par les bactéries dans l'intestin et par la suite, traverse les vaisseaux sanguins en aval pour atteindre leurs principaux organes cibles, notamment les reins. Les dommages causés aux reins peuvent entraîner des complications graves notamment Le syndrome hémolytique urémique (SHU). A ce jour, il n’existe aucun traitement disponible contre le SHU. Les toxines Stx usent du transpo

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14

Edwards, Andrew John. "An NMR isotope labelling analysis of calmodulin interactions with high affinity chiral inhibitors." Thesis, University of Hertfordshire, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.267964.

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15

Liu, Wei. "Semiochemistry of Orgyia and Diatraea lepidopteran species and affinity labelling of 2,3-oxidosqualene cyclase." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/NQ51891.pdf.

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16

Kaminska, Monika. "New activity-based probes to detect matrix metalloproteases." Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS538/document.

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Les Métallo Protéases Matricielles (MMP) en tant qu'endopeptidases à zinc ont une large gamme de fonctions biologiques allant du remodelage tissulaire à la modulation de la réponse cellulaire. Une modification de leur activité protéolytique est souvent associée à de nombreux désordres biologiques. In vivo, ces protéases sont soumises à de nombreuses modifications post-traductionnelles. Elles sont sécrétées sous formes latentes à l'extérieur des cellules pour être ensuite transformées en forme fonctionnelles. Ces dernières sont ensuite inhibées par des inhibiteurs endogènes. En raison de leur s

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17

Cigler, Marko [Verfasser], Kathrin [Akademischer Betreuer] Lang, Kathrin [Gutachter] Lang, and Stephan [Gutachter] Hacker. "Genetically encoding unnatural amino acids: Novel tools for protein labelling and chemical stabilisation of low-affinity protein complexes / Marko Cigler ; Gutachter: Kathrin Lang, Stephan Hacker ; Betreuer: Kathrin Lang." München : Universitätsbibliothek der TU München, 2019. http://d-nb.info/1220322318/34.

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18

Bouthier, de la Tour Claire. "Contribution a l'etude de deux enzymes bacteriennes : la peptidyltransferase ribosomale et la beta cystathionase." Paris 6, 1987. http://www.theses.fr/1987PA066044.

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19

Guillaumot, Nina. "Nouvelles applications et opportunités en protéomique." Thesis, Strasbourg, 2017. http://www.theses.fr/2017STRAF040/document.

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Les objectifs de mes travaux de thèse étaient de développer de nouvelles méthodes d’identification, de caractérisation et de quantification de protéines, mieux adaptées à la diversité des études en protéomique, ce dont la biologie a besoin aujourd’hui. L’analyse protéomique par spectrométrie de masse est apparue comme un outil précieux et pertinent pour évaluer la qualité de l’isolement d’un complexe spécifique, et pour guider les biologistes dans les choix de la stratégie à adopter. La stratégie de marquage de N-terminomique développée a permis de caractériser un processus de maturation biolo

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20

M'Batchi, Bertrand. "Le transporteur de saccharose des tissus foliaires : marquage différentiel, solubilisation et sélectivité." Poitiers, 1987. http://www.theses.fr/1987POIT2028.

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L'utilisation de l'acide para-chloromercuribenzene sulfonique (pcmbs) (reactif peu permeant des groupements thiols) comme marqueur differentiel du transporteur de saccharose des tissus foliaires de feve (vicio faba) revele une forte inhibition selective de l'absorption du saccharose. Cette inhibition est due au blocage du transporteur et non a celui de la pompe a protons fournissant l'energie necessaire au transport. Le point d'impact du pcmbs se situe au niveau du site actif de l'enzyme. Ce marquage differentiel a permis de mettre au point un test de reconnaissance des substrats par le transp

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21

Goulas, Philippe. "Etude de déshydrogénases a NAD(P) : Utilisation en synthèse organique." Université Louis Pasteur (Strasbourg) (1971-2008), 1986. http://www.theses.fr/1986STR13005.

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Etude des problèmes liés à l'utilisation des déshydrogénases a NAD(P) en synthèse organique. Etude, à l'aide d'analogues structuraux du NAD(P), de la 6-phosphogluconate déshydrogénase de Candida Utilis et de l'oestradiol déshydrogénase de placenta humain. Synthese d'un complexe covalent NAD-alcool déshydrogénase du foie de cheval, catalytiquement actif. Purification de la carnitine déshydrogénase de Pseudomonas Putida et la mise en oeuvre pour la synthèse stéréospécifique de la l-carnitine. Mise au point d'une électrode à enzyme spécifique de la l-carnitine

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22

Leelasvatanakij, Leena. "Synthetic strategies for the preparation of affinity label dynorphin A(1-11)NH�� analogues." Thesis, 1996. http://hdl.handle.net/1957/34628.

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23

Maeda, Dean Yoshimasa. "Synthesis and evaluation of affinity labels based on peptide antagonists for delta opioid receptors." Thesis, 1997. http://hdl.handle.net/1957/34507.

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24

Krusemark, Casey J. "Synthetic chemical approaches to proteomics : affinity labeling and protein functional group modification /." 2007. http://www.library.wisc.edu/databases/connect/dissertations.html.

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25

Warth, Rainer K. "Large subunit of vaccinia cirus ribonucleotide reductase : affinity chromatography-based purification and photoaffinity labeling." Thesis, 1993. http://hdl.handle.net/1957/37304.

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Ribonucleoside diphosphate reductase (RR) from vaccinia virus was recently cloned and overexpressed rn Escherichia coli. The amino acid sequence identities of the small and large subunits between the mouse and the vaccinia virus reductase are approximately 80 and 72 percent, respectively. In addition, vaccinia virus RR displays similar complex allosteric regulation to the mouse enzyme and other eukaryotic reductases. The overall activity of the enzyme, which has two subunits (Rl and R2), is regulated through binding to ATP, which activates the enzyme, and dATP which seryes as an inhibitor. Bot

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26

Goulding, Ann Marie. "Biochemical applications of DsRed-monomer utilizing fluorescence and metal-binding affinity." 2011. http://hdl.handle.net/1805/2480.

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Indiana University-Purdue University Indianapolis (IUPUI)<br>The discovery and isolation of naturally occurring fluorescent proteins, FPs, have provided much needed tools for molecular and cellular level studies. Specifically the cloning of green fluorescent protein, GFP, revolutionized the field of biotechnology and biochemical research. Recently, a red fluorescent protein, DsRed, isolated from the Discosoma coral has further expanded the pallet of available fluorescent tools. DsRed shares only 23 % amino acid sequence homology with GFP, however the X-ray crystal structures of the two prot

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27

Dagenais, Pierre. "Purification par affinité et marquage isotopique spécifique pour études d’ARN fonctionnels." Thèse, 2012. http://hdl.handle.net/1866/10197.

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Il existe un lien étroit entre la structure tridimensionnelle et la fonction cellulaire de l’ARN. Il est donc essentiel d’effectuer des études structurales de molécules d’ARN telles que les riborégulateurs afin de mieux caractériser leurs mécanismes d’action. Une technique de choix, permettant d’obtenir de l’information structurale sur les molécules d’ARN est la spectroscopie RMN. Cette technique est toutefois limitée par deux difficultés majeures. Premièrement, la préparation d’une quantité d’ARN nécessaire à ce type d’étude est un processus long et ardu. Afin de résoudre ce problème, notre l

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28

Grilo, Jorge Henrique Ferreira. "Synthesis of photo-affinity labelling reagent to probe HSP90 C-terminal structure-activity relationships." Master's thesis, 2014. http://hdl.handle.net/10451/39277.

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Trabalho Final de Mestrado Integrado, Ciências Farmacêuticas, Universidade de Lisboa, Faculdade de Farmácia, 2014<br>The 90 kDa Heat Shock Protein (Hsp90) is a chaperone protein responsible for regulating the activity of hundreds of structurally diverse client proteins in the cytosol, by providing assistance to their correct folding. In tumours Hsp90 is frequently found to be overexpressed assisting metastable proteins to remain active by stabilizing their conformation and helping them evade the biological degradation mechanisms. Over the past two decades, Hsp90 has become the focus of intens

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