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Artículos de revistas sobre el tema "Agroinfiltratio"

Autor: Grafiati

Publicado: 21 de diciembre de 2024

Última modificación: 31 de julio de 2025

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1

Liu, Pei-Feng, Yanhan Wang, Robert G. Ulrich, et al. "Leaf-Encapsulated Vaccines: Agroinfiltration and Transient Expression of the AntigenStaphylococcal EndotoxinB in Radish Leaves." Journal of Immunology Research 2018 (2018): 1–9. http://dx.doi.org/10.1155/2018/3710961.

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Transgene introgression is a major concern associated with transgenic plant-based vaccines. Agroinfiltration can be used to selectively transform nonreproductive organs and avoid introgression. Here, we introduce a new vaccine modality in which Staphylococcal enterotoxin B (SEB) genes are agroinfiltrated into radishes (Raphanw sativusL.), resulting in transient expression and accumulation of SEBin planta. This approach can simultaneously express multiple antigens in a single leaf. Furthermore, the potential of high-throughput vaccine production was demonstrated by simultaneously agroinfiltrati

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Ambrós, Silvia, Choaa El-Mohtar, Susana Ruiz-Ruiz, et al. "Agroinoculation of Citrus tristeza virus Causes Systemic Infection and Symptoms in the Presumed Nonhost Nicotiana benthamiana." Molecular Plant-Microbe Interactions® 24, no. 10 (2011): 1119–31. http://dx.doi.org/10.1094/mpmi-05-11-0110.

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Citrus tristeza virus (CTV) naturally infects only some citrus species and relatives and within these it only invades phloem tissues. Failure to agroinfect citrus plants and the lack of an experimental herbaceous host hindered development of a workable genetic system. A full-genome cDNA of CTV isolate T36 was cloned in binary plasmids and was used to agroinfiltrate Nicotiana benthamiana leaves, with or without coinfiltration with plasmids expressing different silencing-suppressor proteins. A time course analysis in agroinfiltrated leaves indicated that CTV accumulates and moves cell-to-cell fo

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3

Wang, Zhiquan, Xiaoyang Xu, Longjie Ni, Jinbo Guo, and Chunsun Gu. "Efficient virus-induced gene silencing in Hibiscus hamabo Sieb. et Zucc. using tobacco rattle virus." PeerJ 7 (August 12, 2019): e7505. http://dx.doi.org/10.7717/peerj.7505.

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Background Hibiscus hamabo Sieb. et Zucc. is a semi-mangrove plant used for the ecological restoration of saline-alkali land, coastal afforestation and urban landscaping. The genetic transformation H. hamabo is currently inefficient and laborious, restricting gene functional studies on this species. In plants, virus-induced gene silencing provides a pathway to rapidly and effectively create targeted gene knockouts for gene functional studies. Methods In this study, we tested the efficiency of a tobacco rattle virus vector in silencing the cloroplastos alterados 1 (CLA1) gene through agroinfilt

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Bridgeland, Aya, Sudip Biswas, Nikolaos Tsakirpaloglou, Michael J. Thomson, and Endang M. Septiningsih. "Optimization of gene editing in cowpea through protoplast transformation and agroinfiltration by targeting the phytoene desaturase gene." PLOS ONE 18, no. 4 (2023): e0283837. http://dx.doi.org/10.1371/journal.pone.0283837.

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Cowpea (Vigna unguiculata) is a legume staple widely grown across Sub-Saharan Africa and other tropical and sub-tropical regions. Considering projected climate change and global population increases, cowpea’s adaptation to hot climates, resistance to drought, and nitrogen-fixing capabilities make it an especially attractive crop for facing future challenges. Despite these beneficial traits, efficient varietal improvement is challenging in cowpea due to its recalcitrance to transformation and long regeneration times. Transient gene expression assays can provide solutions to alleviate these issu

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5

Debler, Johannes W., Bernadette M. Henares, and Robert C. Lee. "Agroinfiltration for transient gene expression and characterisation of fungal pathogen effectors in cool-season grain legume hosts." Plant Cell Reports 40, no. 5 (2021): 805–18. http://dx.doi.org/10.1007/s00299-021-02671-y.

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Abstract Key message Modified pEAQ-HT-DEST1 vectors were used for agroinfiltration in legumes. We demonstrate protein expression and export in pea, lentil, and faba bean; however, the method for chickpea was not successful. Abstract Agroinfiltration is a valuable research method for investigating virulence and avirulence effector proteins from pathogens and pests, where heterologous effector proteins are transiently expressed in plant leaves and hypersensitive necrosis responses and other effector functions can be assessed. Nicotiana benthamiana is widely used for agroinfiltration and the char

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6

Dickinson, Christopher C., Alexandra J. Weisberg, and John G. Jelesko. "Transient Heterologous Gene Expression Methods for Poison Ivy Leaf and Cotyledon Tissues." HortScience 53, no. 2 (2018): 242–46. http://dx.doi.org/10.21273/hortsci12421-17.

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Poison ivy [Toxicodendron radicans (L.) Kuntz] is a widely recognized native plant species because of its production of urushiol, which is responsible for delayed contact dermatitis symptoms in humans. Poison ivy is predicted to become both more prevalent and more noxious in response to projected patterns of climate change. Future studies on poison ivy chemical ecology will require reverse genetics to investigate urushiol metabolism. A prerequisite for reverse genetic procedures is the introduction and expression of recombinant DNA into poison ivy tissues. Poison ivy leaves and cotyledons were

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7

Chong, Xinran, Yue Wang, Xiaoyang Xu, et al. "Efficient Virus-Induced Gene Silencing in Ilex dabieshanensis Using Tobacco Rattle Virus." Forests 14, no. 3 (2023): 488. http://dx.doi.org/10.3390/f14030488.

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Ilex dabieshanensis is not only an important ornamental plant, but can also be used to produce Kuding tea, owing to its lipid-lowering and anti-inflammatory medicinal properties. The genetic transformation of I. dabieshanensis is currently difficult, which restricts functional gene studies and molecular breeding research on this species. Virus-induced gene silencing (VIGS) is a powerful tool for determining gene functions in plants. The present study reports the first application of VIGS mediated by a tobacco rattle virus (TRV) vector in I. dabieshanensis. We tested the efficiency of the VIGS

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8

Chiba, Sotaro, Kamal Hleibieh, Alice Delbianco, et al. "The Benyvirus RNA Silencing Suppressor Is Essential for Long-Distance Movement, Requires Both Zinc-Finger and NoLS Basic Residues but Not a Nucleolar Localization for Its Silencing-Suppression Activity." Molecular Plant-Microbe Interactions® 26, no. 2 (2013): 168–81. http://dx.doi.org/10.1094/mpmi-06-12-0142-r.

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The RNA silencing-suppression properties of Beet necrotic yellow vein virus (BNYVV) and Beet soil-borne mosaic virus (BSBMV) cysteine-rich p14 proteins have been investigated. Suppression of RNA silencing activities were made evident using viral infection of silenced Nicotiana benthamiana 16C, N. benthamiana agroinfiltrated with green fluorescent protein (GFP), and GF-FG hairpin triggers supplemented with viral suppressor of RNA silencing (VSR) constructs or using complementation of a silencing-suppressor-defective BNYVV virus in Chenopodium quinoa. Northern blot analyses of small-interfering

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9

Tu, Liqin, Shuhua Wu, Danna Gao, Yong Liu, Yuelin Zhu, and Yinghua Ji. "Synthesis and Characterization of a Full-Length Infectious cDNA Clone of Tomato Mottle Mosaic Virus." Viruses 13, no. 6 (2021): 1050. http://dx.doi.org/10.3390/v13061050.

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Tomato mottle mosaic virus (ToMMV) is a noteworthy virus which belongs to the Virgaviridae family and causes serious economic losses in tomato. Here, we isolated and cloned the full-length genome of a ToMMV Chinese isolate (ToMMV-LN) from a naturally infected tomato (Solanum lycopersicum L.). Sequence analysis showed that ToMMV-LN contains 6399 nucleotides (nts) and is most closely related to a ToMMV Mexican isolate with a sequence identity of 99.48%. Next, an infectious cDNA clone of ToMMV was constructed by a homologous recombination approach. Both the model host N. benthamiana and the natur

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10

Sindarovska, Yana, and Mykola Kuchuk. "Long-Term Potato Virus X (PVX)-Based Transient Expression of Recombinant GFP Protein in Nicotiana benthamiana Culture In Vitro." Plants 10, no. 10 (2021): 2187. http://dx.doi.org/10.3390/plants10102187.

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Plant molecular farming has a great potential to produce valuable proteins. Transient expression technology provides high yields of recombinant proteins in greenhouse-grown plants, but every plant must be artificially agroinfiltrated, and open greenhouse systems are less controlled. Here, we propose to propagate agrobacteria-free plants with high-efficient long-term self-replicated transient gene expression in a well-controlled closed in vitro system. Nicotiana benthamiana plant tissue culture in vitro, with transient expression of recombinant GFP, was obtained through shoot induction from lea

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11

Fan, Xudong, Zunping Zhang, Fang Ren, et al. "Development of a Full-Length Infectious cDNA Clone of the Grapevine Berry Inner Necrosis Virus." Plants 9, no. 10 (2020): 1340. http://dx.doi.org/10.3390/plants9101340.

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Grapevine berry inner necrosis virus (GINV) belongs to the genus Trichovirus in the family Betaflexiviridae. The GINV isolate LN_BETA_RS was obtained from a “Beta” grapevine (Vitis riparia × Vitis labrusca) exhibiting chlorotic mottling and ring spot in Xingcheng, Liaoning Province, China. To verify the correlation between GINV and grapevine chlorotic mottling and ring spot disease, we constructed an infectious cDNA clone of GINV isolate LN_BETA_RS using the seamless assembly approach. Applied treatments of agroinfiltration infectious cDNA confirmed systemic GINV infection of the Nicotianaocci

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Malla, Ashwini, Balamurugan Shanmugaraj, Ashutosh Sharma, and Sathishkumar Ramalingam. "Production of Genistein in Amaranthus tricolor var. tristis and Spinacia oleracea by Expression of Glycine max Isoflavone Synthase." Plants 10, no. 11 (2021): 2311. http://dx.doi.org/10.3390/plants10112311.

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Isoflavonoids, the diverse group of secondary metabolites derived from the phenylpropanoid pathway, are distributed predominantly in leguminous plants. It has received considerable attention in recent days due to its health promoting benefits and is known to prevent certain diseases in humans. These isoflavonoids are synthesized from flavonoid intermediates of phenylpropanoid pathway by the enzyme isoflavone synthase. Metabolic engineering of isoflavonoid biosynthesis in non-legume crop plants could offer the health benefits of these compounds in diverse plant species further contributing for

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13

Chen, Qiang, and Huafang Lai. "Gene Delivery into Plant Cells for Recombinant Protein Production." BioMed Research International 2015 (2015): 1–10. http://dx.doi.org/10.1155/2015/932161.

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Recombinant proteins are primarily produced from cultures of mammalian, insect, and bacteria cells. In recent years, the development of deconstructed virus-based vectors has allowed plants to become a viable platform for recombinant protein production, with advantages in versatility, speed, cost, scalability, and safety over the current production paradigms. In this paper, we review the recent progress in the methodology of agroinfiltration, a solution to overcome the challenge of transgene delivery into plant cells for large-scale manufacturing of recombinant proteins. General gene delivery m

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14

Fuhrmann-Aoyagi, Martina Bianca, Saki Igarashi, and Kenji Miura. "Comparative Evaluation of Transient Protein Expression Efficiency in Tissues across Soybean Varieties Using the Tsukuba System." Plants 13, no. 6 (2024): 858. http://dx.doi.org/10.3390/plants13060858.

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Transient protein expression is a versatile tool with diverse applications and can be used in soybeans to study gene function, obtain mutants, and produce proteins for commercial use. However, soybeans are considered recalcitrant for agroinfiltration. Subsequent studies on soybeans have demonstrated a green fluorescent protein (GFP) expression in seedpods, but not in leaves, using syringe agroinfiltration. To evaluate agroinfiltration-based transient protein expression levels in plant cells, we used the transient expression vector pTKB3 harboring the GFP gene. Using Agrobacterium tumefaciens,

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15

Primasiwi, Dionysia Heviarie, Yekti Asih Purwestri, and Endang Semiarti. "Improving transient gene expression and agroinfiltration‐based transformation effectiveness in Indonesian orchid Phalaenopsis amabilis (L.) Blume." Indonesian Journal of Biotechnology 29, no. 3 (2024): 111. http://dx.doi.org/10.22146/ijbiotech.80555.

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Transient gene expression is an approach used to study transient genes across various species, with infiltration by Agrobacterium tumefaciens (agroinfiltration) being a commonly used method. Agroinfiltration offers a simple and effective means of delivering transgenes into the plant genome. An alternative method for enhancing the quality and productivity of orchids as ornamental plants is genetic modification through agroinfiltration. Although Agrobacterium‐mediated genetic transformation by immersion has been used on the Phalaenopsis amabilis (L.) Blume species of orchid, transformation effic

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16

Vlot, A. Corina, Aymeric Menard, and John F. Bol. "Role of the Alfalfa Mosaic Virus Methyltransferase-Like Domain in Negative-Strand RNA Synthesis." Journal of Virology 76, no. 22 (2002): 11321–28. http://dx.doi.org/10.1128/jvi.76.22.11321-11328.2002.

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ABSTRACT RNAs 1 and 2 of the tripartite genome of alfalfa mosaic virus (AMV) encode the replicase proteins P1 and P2, respectively. P1 contains a methyltransferase-like domain in its N-terminal half, which has a putative role in capping the viral RNAs. Six residues in this domain that are highly conserved in the methyltransferase domains of alphavirus-like viruses were mutated individually in AMV P1. None of the mutants was infectious to plants. Mutant RNA 1 was coexpressed with wild-type (wt) RNAs 2 and 3 from transferred DNA vectors in Nicotiana benthamiana by agroinfiltration. Mutation of H

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17

Angel, Carlos A., Yi-Cheng Hsieh, and James E. Schoelz. "Comparative Analysis of the Capacity of Tombusvirus P22 and P19 Proteins to Function as Avirulence Determinants in Nicotiana species." Molecular Plant-Microbe Interactions® 24, no. 1 (2011): 91–99. http://dx.doi.org/10.1094/mpmi-04-10-0089.

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We have used an agroinfiltration assay for a comparative study of the roles of tombusvirus P22 and P19 proteins in elicitation of hypersensitive response (HR)-like necrosis and the role of P19 in silencing suppression in Nicotiana species. The advantage of agroinfiltration rather than expression in plant virus vectors is that putative viral avirulence proteins can be evaluated in isolation, eliminating the possibility of synergistic effects with other viral proteins. We found that tombusvirus P22 and P19 proteins elicited HR-like necrosis in certain Nicotiana species but, also, that Nicotiana

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18

Annamalai, Padmanaban, Fady Rofail, Darleen A. DeMason, and A. L. N. Rao. "Replication-Coupled Packaging Mechanism in Positive-Strand RNA Viruses: Synchronized Coexpression of Functional Multigenome RNA Components of an Animal and a Plant Virus in Nicotiana benthamiana Cells by Agroinfiltration." Journal of Virology 82, no. 3 (2007): 1484–95. http://dx.doi.org/10.1128/jvi.01540-07.

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ABSTRACT Flock house virus (FHV), a bipartite RNA virus of insects and a member of the Nodaviridae family, shares viral replication features with the tripartite brome mosaic virus (BMV), an RNA virus that infects plants and is a member of the Bromoviridae family. In BMV and FHV, genome packaging is coupled to replication, a widely conserved mechanism among positive-strand RNA viruses of diverse origin. To unravel the events that modulate the mechanism of replication-coupled packaging, in this study, we have extended the transfer DNA (T-DNA)-based agroinfiltration system to express functional g

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Prudhomme, N., R. Pastora, B. Muselius, M. D. McLean, D. Cossar, and J. Geddes-McAlister. "Exposure of Agrobacterium tumefaciens to agroinfiltration medium demonstrates cellular remodelling and may promote enhanced adaptability for molecular pharming." Canadian Journal of Microbiology 67, no. 1 (2021): 85–97. http://dx.doi.org/10.1139/cjm-2020-0239.

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Agroinfiltration is used to treat plants with modified strains of Agrobacterium tumefaciens for the purpose of transient in planta expression of genes transferred from the bacterium. These genes encode valuable recombinant proteins for therapeutic or industrial applications. Treatment of large quantities of plants for industrial-scale protein production exposes bacteria (harboring genes of interest) to agroinfiltration medium that is devoid of nutrients and carbon sources for prolonged periods of time (possibly upwards of 24 h). Such conditions may negatively influence bacterial viability, inf

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20

Demone, Jordan, Mariam Maltseva, Maryam Nourimand, et al. "Scalable agroinfiltration-based production of SARS-CoV-2 antigens for use in diagnostic assays and subunit vaccines." PLOS ONE 17, no. 12 (2022): e0277668. http://dx.doi.org/10.1371/journal.pone.0277668.

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Agroinfiltration is a method used in biopharming to support plant-based biosynthesis of therapeutic proteins such as antibodies and viral antigens involved in vaccines. Major advantages of generating proteins in plants is the low cost, massive scalability and the rapid yield of the technology. Herein, we report the agroinfiltration-based production of glycosylated SARS-CoV-2 Spike receptor-binding domain (RBD) protein. We show that it exhibits high-affinity binding to the SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) and displays folding similar to antigen produced in mammalian ex

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Van der Hoorn, Renier A. L., Franck Laurent, Ronelle Roth, and Pierre J. G. M. De Wit. "Agroinfiltration Is a Versatile Tool That Facilitates Comparative Analyses of Avr9/Cf-9-Induced and Avr4/Cf-4-Induced Necrosis." Molecular Plant-Microbe Interactions® 13, no. 4 (2000): 439–46. http://dx.doi.org/10.1094/mpmi.2000.13.4.439.

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The avirulence genes Avr9 and Avr4 from the fungal tomato pathogen Cladosporium fulvum encode extracellular proteins that elicit a hypersensitive response when injected into leaves of tomato plants carrying the matching resistance genes, Cf-9 and Cf-4, respectively. We successfully expressed both Avr9 and Avr4 genes in tobacco with the Agrobacterium tumefaciens transient transformation assay (agroinfiltration). In addition, we expressed the matching resistance genes, Cf-9 and Cf-4, through agroinfiltration. By combining transient Cf gene expression with either transgenic plants expressing one

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Kaur, Maninder, Pooja Manchanda, Anu Kalia, et al. "Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants." International Journal of Molecular Sciences 22, no. 19 (2021): 10882. http://dx.doi.org/10.3390/ijms221910882.

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Agrobacterium-mediated transformation is one of the most commonly used genetic transformation method that involves transfer of foreign genes into target plants. Agroinfiltration, an Agrobacterium-based transient approach and the breakthrough discovery of CRISPR/Cas9 holds trending stature to perform targeted and efficient genome editing (GE). The predominant feature of agroinfiltration is the abolishment of Transfer-DNA (T-DNA) integration event to ensure fewer biosafety and regulatory issues besides showcasing the capability to perform transcription and translation efficiently, hence providin

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Xiang, Yu, Kishore Kakani, Ron Reade, Elizabeth Hui, and D'Ann Rochon. "A 38-Amino-Acid Sequence Encompassing the Arm Domain of the Cucumber Necrosis Virus Coat Protein Functions as a Chloroplast Transit Peptide in Infected Plants." Journal of Virology 80, no. 16 (2006): 7952–64. http://dx.doi.org/10.1128/jvi.00153-06.

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ABSTRACT Experiments to determine the subcellular location of the coat protein (CP) of the tombusvirus Cucumber necrosis virus (CNV) have been conducted. By confocal microscopy, it was found that an agroinfiltrated CNV CP-green fluorescent protein (GFP) fusion targets chloroplasts in Nicotiana benthamiana leaves and that a 38-amino-acid (aa) region that includes the complete CP arm region plus the first 4 amino acids of the shell domain are sufficient for targeting. Western blot analyses of purified and fractionated chloroplasts showed that the 38-aa region directs import to the chloroplast st

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Muthusamy, Saraladevi, Ramesh R. Vetukuri, Anneli Lundgren та ін. "Transient expression and purification of β-caryophyllene synthase in Nicotiana benthamiana to produce β-caryophyllene in vitro". PeerJ 8 (28 квітня 2020): e8904. http://dx.doi.org/10.7717/peerj.8904.

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The sesquiterpene β-caryophyllene is an ubiquitous component in many plants that has commercially been used as an aroma in cosmetics and perfumes. Recent studies have shown its potential use as a therapeutic agent and biofuel. Currently, β-caryophyllene is isolated from large amounts of plant material. Molecular farming based on the Nicotiana benthamiana transient expression system may be used for a more sustainable production of β-caryophyllene. In this study, a full-length cDNA of a new duplicated β-caryophyllene synthase from Artemisia annua (AaCPS1) was isolated and functionally characteri

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Pillay, Priyen, Karl J. Kunert, Stefan van Wyk, Matome Eugene Makgopa, Christopher A. Cullis, and Barend J. Vorster. "Agroinfiltration contributes to VP1 recombinant protein degradation." Bioengineered 7, no. 6 (2016): 459–77. http://dx.doi.org/10.1080/21655979.2016.1208868.

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King, Jessica L., John J. Finer, and Leah K. McHale. "Development and optimization of agroinfiltration for soybean." Plant Cell Reports 34, no. 1 (2014): 133–40. http://dx.doi.org/10.1007/s00299-014-1694-4.

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Beihaghi, Maria, Hasan Marashi, Abdolreza Bagheri, and Mojtaba Sankian. "Transient Expression of CCL21Chemokine in Tobacco via Agroinfiltration." International Journal of Scientific & Engineering Research 7, no. 10 (2016): 430–35. http://dx.doi.org/10.14299/ijser.2016.10.009.

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Suzaki, Takuya, Mai Tsuda, Hiroshi Ezura, Brad Day, and Kenji Miura. "Agroinfiltration-based efficient transient protein expression in leguminous plants." Plant Biotechnology 36, no. 2 (2019): 119–23. http://dx.doi.org/10.5511/plantbiotechnology.19.0220b.

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Ma, Zhenguo, Jun-Jun Liu, Arezoo Zamany, and Holly Williams. "Transient gene expression in western white pine using agroinfiltration." Journal of Forestry Research 31, no. 5 (2019): 1823–32. http://dx.doi.org/10.1007/s11676-019-00938-5.

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Duc Tien, Nguyen Quang. "Transient Expression of Chi42 Genes from Trichoderma asperellum in Nicotiana benthamiana by Agroinfiltration." International Journal of Agriculture and Biology 26, no. 01 (2021): 177–84. http://dx.doi.org/10.17957/ijab/15.1822.

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The present study reports the transient expression of chi42 genes encoding 42 kDa chitinase from T. asperellum SH16 in N. benthamiana via agroinfiltration. The efficacy of agroinfiltration for chi42 genes including a wild-type gene (Chi42) and two synthetic genes (syncodChi42-1 and syncodChi42-2) was determined. Accordingly, coinfiltration of two vectors pMYV719 carrying one of three genes chi42 and pMYV508 carrying gene p19 expedited the higher expression of recombinant enzymes whose genes were optimized for codon usage in plant tissues. The highest chitinolytic activity of about 290 U/mL was

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Do, Van Giap, Youngsuk Lee, Jeong-Hee Kim, et al. "The Synergistic Effects of Environmental and Genetic Factors on the Regulation of Anthocyanin Accumulation in Plant Tissues." International Journal of Molecular Sciences 24, no. 16 (2023): 12946. http://dx.doi.org/10.3390/ijms241612946.

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Anthocyanin accumulation is responsible for the coloration of apple fruit, and their accumulation depends on the expression of anthocyanin biosynthesis-related genes. Light is an environmental stimulus that induces fruit color by regulating genes involved in the anthocyanin biosynthesis pathway. In this study, the roles of light and genetic factors on fruit coloration and anthocyanin accumulation in apple fruit were investigated. Three genes in the anthocyanin biosynthesis pathway, MdCHS, MdANS, and MdUFGT1, were synthesized and cloned into a viral-based expression vector system for transient

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Ghoshal, Kankana, Jane Theilmann, Ron Reade, Ajay Maghodia, and D'Ann Rochon. "Encapsidation of Host RNAs by Cucumber Necrosis Virus Coat Protein during both Agroinfiltration and Infection." Journal of Virology 89, no. 21 (2015): 10748–61. http://dx.doi.org/10.1128/jvi.01466-15.

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ABSTRACTNext-generation sequence analysis of virus-like particles (VLPs) produced during agroinfiltration of cucumber necrosis virus (CNV) coat protein (CP) and of authentic CNV virions was conducted to assess if host RNAs can be encapsidated by CNV CP. VLPs containing host RNAs were found to be produced during agroinfiltration, accumulating to approximately 1/60 the level that CNV virions accumulated during infection. VLPs contained a variety of host RNA species, including the major rRNAs as well as cytoplasmic, chloroplast, and mitochondrial mRNAs. The most predominant host RNA species encap

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Liu, Lijing, Yiyue Zhang, Sanyuan Tang, et al. "An efficient system to detect protein ubiquitination by agroinfiltration inNicotiana benthamiana." Plant Journal 61, no. 5 (2010): 893–903. http://dx.doi.org/10.1111/j.1365-313x.2009.04109.x.

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Heidari-Japelaghi, Reza, Mostafa Valizadeh, Raheem Haddad, Ebrahim Dorani-Uliaie та Mokhtar Jalali-Javaran. "Production of bioactive human IFN-γ protein by agroinfiltration in tobacco". Protein Expression and Purification 173 (вересень 2020): 105616. http://dx.doi.org/10.1016/j.pep.2020.105616.

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Deng, Xianbao, Jani Kelloniemi, Tuuli Haikonen, et al. "Modification of Tobacco rattle virus RNA1 to Serve as a VIGS Vector Reveals That the 29K Movement Protein Is an RNA Silencing Suppressor of the Virus." Molecular Plant-Microbe Interactions® 26, no. 5 (2013): 503–14. http://dx.doi.org/10.1094/mpmi-12-12-0280-r.

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Tobacco rattle virus (TRV) has a bipartite, positive-sense single-stranded RNA genome and is widely used for virus-induced gene silencing (VIGS) in plants. RNA1 of TRV that lacks the gene for the cysteine-rich 16K silencing-suppression protein infects plants systemically in the absence of RNA2. Here, we attempted to engineer RNA1 for use as a VIGS vector by inserting heterologous gene fragments to replace 16K. The RNA1 vector systemically silenced the phytoene desaturase (PDS) gene, although less efficiently than when the original VIGS vector system was used, which consists of wild-type RNA1 a

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Acanda, Yosvanis, Stacy Welker, Vladimir Orbović, and Amit Levy. "A simple and efficient agroinfiltration method for transient gene expression in Citrus." Plant Cell Reports 40, no. 7 (2021): 1171–79. http://dx.doi.org/10.1007/s00299-021-02700-w.

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Kopertekh, Lilya, and Joachim Schiemann. "Agroinfiltration as a Tool for Transient Expression of cre Recombinase in vivo." Transgenic Research 14, no. 5 (2005): 793–98. http://dx.doi.org/10.1007/s11248-005-8293-7.

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38

Matsuo, Kouki, Noriho Fukuzawa, and Takeshi Matsumura. "A simple agroinfiltration method for transient gene expression in plant leaf discs." Journal of Bioscience and Bioengineering 122, no. 3 (2016): 351–56. http://dx.doi.org/10.1016/j.jbiosc.2016.02.001.

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Carvalho, Raquel F., Sofia D. Carvalho, Kevin O’Grady, and Kevin M. Folta. "Agroinfiltration of Strawberry Fruit — A Powerful Transient Expression System for Gene Validation." Current Plant Biology 6 (October 2016): 19–37. http://dx.doi.org/10.1016/j.cpb.2016.09.002.

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40

van Poppel, Pieter M. J. A., Jun Guo, Peter J. I. van de Vondervoort, et al. "The Phytophthora infestans Avirulence Gene Avr4 Encodes an RXLR-dEER Effector." Molecular Plant-Microbe Interactions® 21, no. 11 (2008): 1460–70. http://dx.doi.org/10.1094/mpmi-21-11-1460.

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Resistance in potato against the oomycete Phytophthora infestans is conditioned by resistance (R) genes that are introgressed from wild Solanum spp. into cultivated potato. According to the gene-for-gene model, proteins encoded by R genes recognize race-specific effectors resulting in a hypersensitive response (HR). We isolated P. infestans avirulence gene PiAvr4 using a combined approach of genetic mapping, transcriptional profiling, and bacterial artificial chromosome marker landing. PiAvr4 encodes a 287-amino-acid-protein that belongs to a superfamily of effectors sharing the putative host-

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41

Wang, Yanfang, Yanzi Zhang, Chongjing Dai, et al. "The Establishment of Two Efficient Transformation Systems to Manipulate and Analyze Gene Functions in Quinoa (Chenopodium quinoa Willd.)." Journal of Advances in Biology & Biotechnology 26, no. 6 (2023): 20–31. http://dx.doi.org/10.9734/jabb/2023/v26i6639.

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Quinoa (C. quinoa) is considered a gluten-free food with abundant nutrients and high tolerance to multiple abiotic stresses, which is the potential to become a major crop in future. Genetic manipulation will provide powerful tools to investigate the function and mechanism of those important genes in the regulation of quinoa development and stress responses, and further improve the quinoa in the field. However, the efficient plant transformation system for quinoa has not been well developed yet. Here, we established two rapid and efficient transformation systems for quinoa by using hairy roots

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42

Wang, Yaqin, Yu Song, Yongzhi Wang, Mengji Cao, Tao Hu, and Xueping Zhou. "Discovery and Characterization of a Novel Ampelovirus on Firespike." Viruses 12, no. 12 (2020): 1452. http://dx.doi.org/10.3390/v12121452.

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A novel RNA virus was identified in firespike (Odontonema tubaeforme) plants exhibiting leaf curling and chlorosis. The molecular features of the viral genomic RNA and proteins resemble those of ampeloviruses. Based on sequence comparisons and phylogenetic analysis, we propose a new species in the genus Ampelovirus, which we have tentatively named Firespike leafroll-associated virus (FLRaV). Bioassays showed that the virus is mechanically transmissible to Nicotiana benthamiana. In addition, a full-length cDNA clone of FLRaV could successfully infect N. benthamiana via agroinfiltration.

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43

Fischer, Rainer, Carmen Vaquero‐Martin, Markus Sack, Jürgen Drossard, Neil Emans, and Ulrich Commandeur. "Towards molecular farming in the future: transient protein expression in plants." Biotechnology and Applied Biochemistry 30, no. 2 (1999): 113–16. http://dx.doi.org/10.1111/j.1470-8744.1999.tb00900.x.

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Molecular farming in plants can be achieved by stable or transient expression of a recombinant protein. Transient expression of recombinant proteins in plants can rapidly provide large amounts of the proteins for detailed characterization. It is fast, flexible and can be carried out at field scale using viral vectors, but it lacks the increases in production volume that can be achieved easily with stable transgenic crops. This review article focuses on discussing the applications of transient expression using viral vectors, biolistic methods or agroinfiltration.

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Minh Hang, Nguyen Thi, Ho Thi Thuong, Nguyen Thu Giang, Pham Bich Ngoc, Nguyen Trung Nam, and Chu Hoang Ha. "OPTIMIZATION OF EXPRESSION CONDITIONS OF GENE ENCODING ANTIGEN M OF PRRSV IN LEAVES OF NICOTIANA BENTHAMIANA BY AGROINFILTRATION METHOD." Vietnam Journal of Biotechnology 16, no. 2 (2018): 293–300. http://dx.doi.org/10.15625/1811-4989/16/2/13440.

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Reproductive disorders and respiratory swine (Porcine Reproductive and Respiratory Syndrome - PRRS) is an infectious disease caused by the PRRS virus (PRRSV), spread fast speeds, causing mass death when infected pigs. M protein is one of the major structural protein of the PRRSV, has a molecular weight of about 19 kDa, encoded by the open reading frame 6 (ORF6), which is used to design a subunit vaccine against back PRRSV. In this study, conditions for transient expression of the gene encoded the M protein of PRRSV in leaves of the Nicotiana benthamiana by agroinfiltration method were determin

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45

SONG, Guo-qing, and Ken-ichi YAMAGUCHI. "Efficient Agroinfiltration-mediated Transient GUS Expression System for Assaying Different Promoters in Rice." Plant Biotechnology 20, no. 3 (2003): 235–39. http://dx.doi.org/10.5511/plantbiotechnology.20.235.

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Park, Sang-Ho, and Kook-Hyung Kim. "Agroinfiltration-based Potato Virus X Replicons to Dissect the Requirements of Viral Infection." Plant Pathology Journal 22, no. 4 (2006): 386–90. http://dx.doi.org/10.5423/ppj.2006.22.4.386.

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Xiong, Yongao, Qiongyu Li, Muchena Kailemia, Carlito Lebrilla, Somen Nandi, and Karen McDonald. "Glycoform Modification of Secreted Recombinant Glycoproteins through Kifunensine Addition during Transient Vacuum Agroinfiltration." International Journal of Molecular Sciences 19, no. 3 (2018): 890. http://dx.doi.org/10.3390/ijms19030890.

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Palanichelvam, Karuppaiah, Anthony B. Cole, Monir Shababi, and James E. Schoelz. "Agroinfiltration of Cauliflower mosaic virus Gene VI Elicits Hypersensitive Response in Nicotiana Species." Molecular Plant-Microbe Interactions® 13, no. 11 (2000): 1275–79. http://dx.doi.org/10.1094/mpmi.2000.13.11.1275.

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Cauliflower mosaic virus strain W260 induces hypersensitive response (HR) in Nicotiana edwardsonii and systemic cell death in N. clevelandii. In contrast, the D4 strain of Cauliflower mosaic virus evades the host defenses in Nicotiana species; it induces chlorotic primary lesions and a systemic mosaic in both hosts. Previous studies with chimeric viruses had indicated that gene VI of W260 was responsible for elicitation of HR or cell death. To prove conclusively that W260 gene VI is responsible, we inserted gene VI of W260 and D4 into the Agrobacterium tumefaciens binary vector pKYLX7. Agroinf

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Wieczorek, Przemysław, Marta Budziszewska, and Aleksandra Obrępalska-Stęplowska. "Construction of infectious clones of tomato torrado virus and their delivery by agroinfiltration." Archives of Virology 160, no. 2 (2014): 517–21. http://dx.doi.org/10.1007/s00705-014-2266-1.

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Natorajan, D., HY Yong, and I. Zainal. "Feasibility analysis of leaf disc samples produced via agroinfiltration for promoter trapping studies." Emirates Journal of Food and Agriculture 22, no. 6 (2010): 448. http://dx.doi.org/10.9755/ejfa.v22i6.4662.

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