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1
Georges, Anne Marie. "Le processus astériforme : une clé pour appréhender la clinique du sujet victime de violence". Thesis, Lyon 2, 2011. http://www.theses.fr/2011LYO20020.
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Une situation de violence intentionnelle infligée de l’extérieur, propulse le sujet sur une scène qui infiltre le présent d’une actualisation du passé et provoque un vertige abyssal qui invite à une figurabilité interne de traumas primordiaux conjugués à l’évènementiel. Cette expérience a valeur d’épreuve et force la mise au travail psychique.En s’appuyant sur la clinique des violences conjugales et de celle des victimes d’agression « sur la voie publique », trois axes de recherche sont proposés :- Les processus de répétition agis, cauchemardés ou oralisés, agents de changement.- Le corps et la psyché en tant que surfaces de réception et de figuration des violences- La prégnance du détail comme moteur essentiel du traitement du trauma.A la suite d’une agression, le processus astériforme fournit des points d’accroche pour faire bord au risque d’anéantissement grâce à un mouvement de reprise, par diffraction, focalisation, et unification-différenciation. Faute de pouvoir se représenter l’irreprésentable, à savoir le Réel de la mort, le sujet trace une esquisse, et met en place les prémices d’une figuration dont il va pouvoir se saisir. Après l’effondrement se dessine une possibilité de reconstructionIn situations where violence is deliberately inflicted from outside, the victim is propelled into a position where the present is infiltrated by an actualisation of the past and provokes a deep confusion linked to internal experiences of past events and primordial traumas. This experience has a test value and forces the beginning of the psychical work.Three axes of research are proposed in considering the victim experience in domestic as well as public violence:- To consider using acted, nightmares and verbalisation process of repetition as agents of change.- The body and the psyche as surfaces for reception and figuration of violence.- The use of expressed salient details as a driving force of the trauma treatment.Following an attack, the psychic asterism approach provides reference points and is able to promote the recovery process by way of diffraction, focusing an reunifying. In an attempt to represent the unrepresentable together with the reality of death, the victim will start to sketch the beginning of a figuration which he will be able to grab.Following a collapse, there begins to appear a possibility of rebuilding
2
Kenick, Erica N. "Asterisms". FIU Digital Commons, 2015. http://digitalcommons.fiu.edu/etd/1935.
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ASTERISMS is a collection of lyric poetry that seeks to express a sense of awe for the natural world by exploring themes of science, art, and the self. By combining physics and metaphysics, scientific terminology and musings on love, ASTERISMS argues that these seemingly-disparate fields of knowledge can harmonize in unexpected ways.
In its style, the collection draws from the works of Dorianne Laux, Pablo Neruda, and Annie Dillard. Most of the poems are written in free-version and are tied together by images of astronomy and wilderness, both modern and prehistoric. Poems about classical music appear as interludes meant to complement others concerned with science and technology, as music too has its own invented language.
Like asterisms - ancient inventions meant to personalize the expansive mystery of the night sky - this collection seeks to admire, if not completely understand, our place in the natural world and cosmos beyond.
3
Guerra, Carlos Humberto Martins Chagas. "Gateway SIP - Asterisk". Master's thesis, Universidade de Évora, 2012. http://hdl.handle.net/10174/15430.
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Esta tese consiste num estudo realizado sobre a tecnologia VoIP (Voice over IP) com aplicação
dentro da área dos PBX(Private Branch eXchange) e Gateways VoIP Open Source.
Em primeiro lugar foram abordados os conceitos, requisitos e elementos associados a esta
tecnologia bem como a sua interligação com outras tecnologias de comunicação como a PSTN
(Public Switched Telephone Network) , ISDN (Integrated Services Digital Network) ou GSM
(Groupe Special Mobile).Al ém disso, foi efectuado um estudo teórico e prático sobre o software
Open Source Asterisk, tendo com objectivo explorar o seu modo de funcionamento e funcionalidades
disponíveis, passiveis de serem utilizadas em ambiente empresarial.
Por fim, foi desenvolvida uma solução assente neste tipo de tecnologia na empresa Clidis
- Laboratório de Análises Clinicas de Sines, onde ficou provado que a implementação de
PBX/Gateways VoIP através de software Open Source e uma alternativa viável às reais necessidades
de comunicação das empresas; ABSTRACT: This thesis is a study on VoIP (Voice over IP) application within the area of PBX (Private
Branch eXchange) and VoIP Open Source Gateways.
Firstly were addressed the concepts, requirements and elements associated with this technology
and its interconnection with other communications technologies such as PSTN (Public
Switched Telephone Network), ISDN (Integrated Services Digital Network) or GSM (Groupe
Special Mobile). Furthermore, a study was carried out on the theoretical and practical Asterisk
Open Source software, with the aim to explore its operation and features available, liable to be
used in business environment.
Finally, we developed in the company Clidis (Laboratory of Clinical Analyses in Sines) a
solution based on this technology, where it was proved that the implementation of PBX / VoIP
Gateways through Open Source software is a viable alternative to the real needs of business
communication.
4
Hörlin, Sara. "ToIP functionality in Asterisk". Thesis, Linköping University, Department of Computer and Information Science, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-9761.
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In the thesis the advantages with Text over IP (ToIP) is explained and it is motivated why it is a good idea to integrate this in Asterisk. It also presents an implementation of a ToIP extension in Asterisk.
ToIP means communicating over a network based on Internet protocols with real-time text. Real-time text means a character is sent to the receiving terminal as soon the sender has typed it or with a small delay.
In the thesis IM and ToIP is compared in a survey. The result point at IM is not better than ToIP even though it is much more commonly used. VoIP can not replace ToIP either because there are occasions when ToIP is better for instance if the person using it is deaf or if a person want to make a private conversation in a noisy room.
Asterisk is an IP-PBX. PBX stands for Private Branch Exchange which means a private telephone system which is part of a larger network system that exchange information.
An IP-PBX is a PBX based on the Internet. Asterisk and many other IP-PBX can also exchange calls between the PSTN ant the Internet. By including ToIP in Asterisk it will be possible to exchange ToIP calls.
The implementation described is not only including ToIP in Asterisk but also a translation function between the text format called t140 and another text format called t140 with redundancy.
The idea is to extend the translation function in the future to more text formats.
5
Vlk, Bronislav. "Možnosti videokonferencí v PBX Asterisk". Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2012. http://www.nusl.cz/ntk/nusl-219738.
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This thesis deals with the possibilities of video conferencing in Asterisk PBX and their use in practice. They also described the contingencies and how its configuration. Particular attention is paid to the protocols SIP, IAX and H.323, which are described in one of the chapters. The thesis was created by the Asterisk PBX, which demonstrates cooperation with videoconferencing clients. The thesis describes the configuration files so that the central set. Conclusion the work assesses the use of codecs for different clients.
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Binder, Tomáš. "Správa a konfigurace VoIP ústředny Asterisk". Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2008. http://www.nusl.cz/ntk/nusl-217252.
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This diploma dissertation is dealing with the VoIP software exchange Asterisk. In the dissertation there are described its abilities and possible ways of its configuration. Special attention is given to the signalling protocol SIP, which is described in one of the chapters. Within this dissertation a dial plan, which demonstrates the technique of dial plan creating, was created. Within the boundaries of the dialplan following services could be found: a voicemail, conference, Interactive Voice Response and call queues. Configuration files, with the help of which the exchange is configurated, are described in my dissertation as well. Finally, three laboratory assignments for purposes of the subject Multimedia Services are mentioned. Their main aim is to familiarise students with the creation of SIP accounts in the exchange, their mutual connections, defining the Interactive Voice Response and forming a new call centre.
7
Benýšek, Jiří. "Vazba GSM modemu na PBX Asterisk". Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2010. http://www.nusl.cz/ntk/nusl-218255.
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Short Message Service (shortly SMS) is the most widely used type of communication systems. The main advantages are that allow a fast exchange of messages between devices, a very good availability through GSM and a reasonable price. Nowadays the SMS service support has expanded to include other technologies such as a service of the information navigation and the remote connection. The master‘s thesis concentrates on the Short Message Service, deals with basic principles and statements using by this service. The topic of the thesis is software PBX Asterisk and its possibility of SMS implementation, especially verification of SMS processing goes through the PSTN. After the basic introduction the master‘s work deals with the installation and configuration of the server. The main focus is on an installation of the operating system with an additional pack including necessary libraries and modules for a correct working of the server. The following section is paying attention to the Asterisk server configuration, especially a hardware card installation which is necessary for a connection with analog telephones, done by Bluetooth connections, set up user’s profiles of the SIP protocol and create a dial plan. This is followed by a verification of SMS option of the implementation and communication with GSM modem which is used as a gate for an exchange SMS between PSTN and GSM network. The last chapter of this master‘s thesis comes with the aimed results.
8
Bednář, Vít. "Implementace protokolu SIP v PBX Asterisk". Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2017. http://www.nusl.cz/ntk/nusl-317016.
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The thesis compares native SIP stack with PJSIP stack in the open source telephone private branch exchange (PBX) Asterisk. First, there are described both SIP protocol and Asterisk application. Subsequently, the architecture, new function support and the stacks setting possibilities are explored. For different exchange scenarios several commented configuration files are presented. The stacks are tested using Spirent TestCenter C1 software thereafter. In conclusion, selected properties are assessed and new PJSIP stack benefits are summarized. In addition, the laboratory assignment is attached.
9
Melichar, Ondřej. "Pobočková VoIP ústředna Asterisk a její nástavby". Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2018. http://www.nusl.cz/ntk/nusl-377036.
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This master’s thesis delves into the possibilities of the open-source Private Branch Exchange Asterisk, elaborates on its features and compares it with several other distros. The term SIP stack is explained here with the mention of two of its representatives. Further in the thesis, the security risks of the VoIP technology are explained, and specific attacks are described and then realized. As a part of the testing process, the possibilities of a custom module and its following implementation are explored, as well as the portability between the individual distros and its proper functioning.
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Depiak, Petr. "Bilingový systém a monitorování hovorů pro PBX Asterisk". Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2010. http://www.nusl.cz/ntk/nusl-218271.
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This master's thesis is focused on developement of billing system with the options of monitoring individual calls for software exchange Asterisk. Billing of calls is adaptible with the help of group of individual rules, consisting of tariff impulses, numerical prefix, with help of outgoing trunk and cost of the billed unit. The first part of this work is focused on instalation, configuration and preparation of individual components of the billing system. In this work is explained the architecture of the billing system and highlighted the purpose of work of the model database. Next we focused on the purpose and the principal system invidual function of the system including solution. At last there is a simple manual to operate the system with the help of created web interface.
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Bílek, Petr. "Měření kvality telefonních hovorů u pobočkové ústředny Asterisk". Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2011. http://www.nusl.cz/ntk/nusl-218970.
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This thesis contains description of the present methods and algoritms for measuring the quality of telephone call on the PBX Asterisk. Further develop the concept of a system for measuring and implementing the call embbeded system VOIPAC PXA270M. Based on theoretical analysis is carried out discussions between C, Java and PHP,which results in the selection of appropriate programming language suitable for implementation in embedded system. The concept is realized by creating an application written in a particular programming language. Part of this work is verification created application using experimental measurments of telecommunications system. The results of experimental measurements are discussed in the final evaluation.
12
Jakubíček, Michal. "Zvukový kodek s podporou zabezpečení pro PBX Asterisk". Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2015. http://www.nusl.cz/ntk/nusl-220422.
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This thesis is focused on the design of secured audio codec for Asterisk PBX. The first chapter is focused on the basic division of traditional PBX producers and the open source PBX. The second chapter explains the structure of Asterisk PBX and its fundamental difference from a traditional PBX. Asterisk is based on components called modules, therefore the work also deals with the most important modules for operation of exchanges and their division of terms of support and dividing by the type of application and their properties. In this chapter there are described in more detail audio codec A-law and u-law. The third chapter contains simple instructions to get your orientation in the construction of the module for Asterisk PBX and this guide is accompanied by a simple example of creating a module demonstration of his method of translation, commissioning and loaded into Asterisk. Simulation of voice security is in the fourth chapter which provides a description of the proposed security solutions with subsequent implementation in Simulink. This simulation verifies the functionality of the solution proposed security phone call. In the fifth chapter outlines the historical use of encryption techniques primarily mirroring the spectrum and time division signal and comparing them with current modern digital technics. In the last sixth chapter is the actual implementation audio codec module with encryption.
13
Ruddy, Conal. "The structure of spectral isometries on C[asterisk]-algebras". Thesis, Queen's University Belfast, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.437542.
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NAPOLITANO, FILLIPE MACHADO PINTO. "A SIMULATION–BASED VALIDATION STRATEGY TO I ASTERISK MODELS". PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO, 2009. http://www.maxwell.vrac.puc-rio.br/Busca_etds.php?strSecao=resultado&nrSeq=15000@1.
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PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIROO entendimento da organização antes de iniciar o desenvolvimento dos sistemas organizacionais tem se mostrado bastante eficaz na elicitação de requisitos. Neste contexto, a utilização dos conceitos de intencionalidade através do Framework i asterisco tem sido amplamente empregada por pesquisadores e em algumas empresas. Porém, o uso do framework i asterisco para a modelagem da intencionalidade organizacional carrega a complexidade intrínseca dos modelos. Sendo assim, este trabalho tem como principal objetivo elaborar uma estratégia baseada em simulação para ajudar o engenheiro de requisitos a validar os modelos em i asterisco elicitados junto aos interessados, sem que os mesmos precisem absorver a complexidade inerente a esses modelos. São apresentados também os resultados obtidos com a aplicação desta estratégia em um estudo de caso.
The understanding of the organization before starting the development of organizational systems has been very effective in requirements elicitation. In this context, the use of intentionality concepts through the i asterisk framework has been widely used by researchers and by some companies. However, the use of i asterisk framework to modeling organizational intentionality brings the inherent complexity of the models. Thus, this work has the main objective to develop a simulation–based strategy to help the requirements engineer to validate the i asterisk models elicitated with the stakeholders, without absorbing the inherent complexity of these models. We also present the results of the strategy implementation in a case study.
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Halamík, Zdeněk. "Možnosti vazby softswitche Asterisk na pobočkové ústředny 4. generace". Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2008. http://www.nusl.cz/ntk/nusl-217329.
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This master’s thesis dissertate the possibilities of the linkage between Asterisk softswitch and the 4th generation private branch exchange. This should create a new generation’s network, so-called NGN, by the convergence of existing telecommunication networks with an IP computer network. This master’s thesis is divided into several chapters. In introduction is described the evolution of the private branch exchanges as well as the principles of the voice digitizing, codecs and signaling commonly used in both TDM and VoIP networks. The main aim of this project is the configuration of Asterisk software exchange for connection with PBX Alcatel 4400 as well as public phone network PSTN. Another goal of this master’s thesis was the configuration of Alcatel PBX and diagnostics of CCS and CAS signaling on E1 interface. In conclusion there are summarized advantages of NGN networks and their utilization in the future.
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Janíček, Martin. "Možnosti implementace signalizačního systému číslo 7 v PBX Asterisk". Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2009. http://www.nusl.cz/ntk/nusl-218068.
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Semestral project describes signaling system number 7, it's settings options and options of signaling over IP networks, especially two transport protocols SCTP and BICC for signaling SS7 over IP. Analyses kinds of implemetations of this signaling system to the Asterisk PBX with TDM E1 card support. Further part of this work is dedicated to the open source implementations libss7 of Digium and chan_ss7 which is currently developed by Dicea. Describes in detail their installation to the open source PBX Asterisk including testing of both and comparing these two open source solutions. Last part is focused on realization of gateway which converts communication from TDM network to IP network. For this part, three computers are used. First as SS7 signalling end softswitch, second as SIP signalling end softswitch and last as gateway between them. This gate works as interface between SS7 signalling and SIP signalling. Testing call was realized successfully for both directions.
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Stračár, Ivan. "Implementace jednoduché pobočkové ústředny na OpenWRT". Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2014. http://www.nusl.cz/ntk/nusl-220608.
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The diploma thesis deals with the system OpenWRT. Installing this system on the router Siemens Gigaset SX762. Describes how to compile and upload the simple package helloworld into this system. The package was tasked invitation simple phrase "Hello World" to the system console of OpenWRT. Package only serve to show that the system OpenWRT allows users to customize it according to their needs. After that it was installed PBX Asterisk into the system OpenWRT. Proper functioning of PBX Asterisk has been verified to make a call between two software phones ZoiPer. Furthermore, the work described telephony application programming interface (TAPI). Some of its fun- ctions, interfaces and packages needed to communicate with the system OpenWRT. In conclusion, the presented test topology and verify the operation of making calls between analog as well as softphones.
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Vinzent, Markus. "Asterius von Kappadokien : die theologischen Fragmente /". Leiden ; New-York ; Köln : E. J. Brill, 1993. http://catalogue.bnf.fr/ark:/12148/cb37427047s.
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Kulakovskis, Darius. "Balso kokybės vertinimo modulio „Asterisk" VoIP žinybinei stočiai kūrimas ir tyrimas". Master's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2014~D_20140611_081442-70060.
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Baigiamajame magistro darbe sukurtas balso kokybės vertinimo modulis „Asterisk“ VoIP žinybinei stočiai ir atliktas sukurto modulio tyrimas. Atlikta SIP protokolo architektūros apžvalga, išnagrinėti balso kokybės vertinimo būdai bei įrankiai. Apžvelgtas balso kokybės vertinimo būdų palyginimas mokslinėje literatūroje. Pasinaudojus „libpcap“ biblioteka sukurtas balso kokybės vertinimo modulis. Sukurtas modulis analizuoja RTCP paketus ir vertina balso kokybę pagal ITU-T E modelį. Atliktas balso kokybės tyrimas naudojant sukurtą modulį, o rezultatai palyginti su PESQ algoritmu gautais rezultatais. Atliekant balso kokybės tyrimą buvo nagrinėjami trys pagrindiniai veiksniai - vėlinimas, paketų praradimas ir vėlinimo laikų švytavimas. Nustatyta, kad daugeliu atvejų balso kokybės vertinimo moduliu gauti parametrai gali būti laikomi patikimais balso kokybės indikatoriais. Darbą sudaro 7 dalys: įvadas ir užduoties analizė, SIP architektūros apžvalga, balso kokybės vertinimo būdų bei įrankių apžvalga, balso kokybės vertinimo modulis, balso kokybės tyrimas naudojant sukurtą modulį, rezultatų apibendrinimas, literatūros ir informacinių šaltinių sąrašas.In this master thesis research of voice quality estimation system for Asterisk PBX was performed. SIP architecture was reviewed and voice quality estimation methods and tools were described. Scientific literature about voice quality estimation methods was reviewed. Voice quality estimation system was created by using “libpcap” library. The created system uses RTCP packets to estimate voice quality based on ITU-T E-model. Research of voice quality was performed by using the created system and the results were compared to PESQ algorithm results. Three most important parameters were considered during voice quality estimation - delay, packet loss and jitter. Research has confirmed that in most cases measurements performed with the created system can be considered reliable voice quality parameters. Structure consists of 7 chapters: introduction and task analysis, review of SIP architecture, review of voice quality estimation methods and tools, voice quality estimation system, research of voice quality by using the created system, summary of thesis results, references.
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Wiley, Jeffrey A. "Functionality minimization analysis of Asterisk for future use in secure environments". Thesis, Monterey, California : Naval Postgraduate School, 2009. http://edocs.nps.edu/npspubs/scholarly/theses/2009/Sep/09Sep%5FWiley.pdf.
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Thesis (M.S. in Computer Science)--Naval Postgraduate School, September 2009.Thesis Advisor(s): Irvine, Cynthia E. "September 2009." Description based on title screen as viewed on November 5, 2009. Author(s) subject terms: Asterisk, MYSEA, Open-source PBX, VoIP, Voice over Internet Protocol, Voice mail. Includes bibliographical references (p. 84-87). Also available in print.
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Kårehed, Jesper. "Evolutionary Studies in Asterids Emphasising Euasterids II". Doctoral thesis, Uppsala University, Department of Systematic Botany, 2002. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2696.
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This thesis deals with evolutionary relationships within the asterids, a group of plants comprising about one-third of all flowering plants.
Two new families are recognised: Pennantiaceae and Stemonuraceae. The woody Pennantia from New Zealand and Australia is the sole genus of Pennantiaceae. Stemonuraceae consist of a dozen woody genera with a pantropical distribution and a centre of diversity in South East Asia and the Malesian islands. They are characterised by long hairs on their stamens and/or fleshy appendages on their fruits. Both families were formerly included in Icacinaceae. While Pennantiaceae are unrelated to any of the former Icacinaceae and placed in the order Apiales, other former Icacinaceae genera are related to Cardiopteris, a twining herb from South East Asia and Malesia. The monogeneric family Cardiopteridaceae is enlarged as to include also these. Cardiopteridaceae and Stemonuraceae are sister groups and placed in Aquifoliales. The three other families of Aquifoliales are monogeneric and closely related. The Asian Helwingiaceae and the Central/South American Phyllonomaceae are suggested to be merged into Aquifoliaceae (hollies). The genera of Icacinaceae in the traditional sense not placed in any of the above families (all euasterids II) are members of early diverging lineages of the euasterids I and possibly included in the order Garryales.
The three woody Australasian families Alseuosmiaceae, Argophyllaceae, and Phellinaceae are confirmed as members of Asterales, despite traditional placements not close to that order. They are, moreover, supported as each other’s closest relatives.
The results are based mainly on parsimony analysis of DNA sequence data, but morphological studies have revealed characters in support for the molecularly based conclusions. The gene that has provided most new information is the chloroplast ndhF gene. The results are, however, drawn from combined analyses of sequences from one or several additional genes (atpB, matK, rbcL, 18S rDNA). The data have also been explored with Bayesian analysis, a statistical, model-based method that most recently has been developed for phylogeny reconstruction.
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Rouvière, Nicolas Ory Pascal. "Asterix ou Les lumières de la civilisation". Paris : Presses universitaires de France : "Le Monde, 2006. http://catalogue.bnf.fr/ark:/12148/cb40090103n.
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Brighi, Giada. "Host mi? Asterix und Obelix auf bairisch". Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2014. http://amslaurea.unibo.it/7071/.
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La tesi analizza il dialetto bavarese sulla base di un confronto fra il fumetto di Asterix e Obelix in bavarese e tedesco standard.
Die vorliegende Bachelorarbeit ist eine Analyse des bairischen Dialekts. Der Ausgangspunkt ist der Comic von Asterix und Obelix auf Bairisch.
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Chengegowda, Venkatesh. "Analysis of Queues for Interactive Voice and Video Response Systems : Two Party Video Calls". Thesis, KTH, Kommunikationssystem, CoS, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-102451.
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Video conversation on mobile devices is popularizing with the advent of 3G. The enhanced network capacity thus available enables transmission of video data over the internet. It has been forecasted by several VOIP service organizations that the present IVR systems will evolve into Voice and Video Response (IVVR) Systems. However, this evolution has many technical challenges on the way. Architectures to implement queuing systems for video data and standards for inter conversion of video data between the formats supported by calling parties are two of these challenges. This thesis is an analysis of queues and media transcoding for IVVRs. A major effort in this work involves constructing a prototype IVVR queuing system. The system is constructed by using an open source server named Asterisk and MySql database. Asterisk is a SIP based Public Exchange Server (PBX) and also a development environment for VOIP based IVRs. Functional scenarios for SIP session establishment and the corresponding session setup times for this queueing model are measured. The results indicate that the prototype serves as a sufficient model for a queue, although a significant delay is introduced for session establishment. The work also includes analysis of integrating DiaStar™, is a SIP based media transcoding engine to this queue. However, this system is not complete to function with DiaStar for media translation. The study concludes with a mention of the areas for future work on this particular system and the general state of IVVR queuing systems in the industry.Videosamtal på mobila enheter är popularisera med tillkomsten av 3G. Den förbättrade nätkapacitet så tillgänglig möjliggör överföring av videodata över Internet. Det har prognos av flera VOIP serviceorganisationer att de nuvarande IVR-system kommer att utvecklas till röst och video Response (IVVR) System. Dock har denna utveckling många tekniska utmaningar på vägen. Arkitekturer för att genomföra kösystem för videodata och standarder för bland konvertering av videodata mellan format som stöds för uppringande är två av dessa utmaningar. Denna avhandling är en analys av köer och media kodkonvertering för IVVRs. En stor insats i detta arbete innebär att bygga en prototyp IVVR kösystem. Systemet är konstruerat med hjälp av en öppen källkod-server som heter Asterisk och MySQL-databas. Asterisk är en SIP-baserad Public Exchange Server (PBX) och även en utvecklingsmiljö för VOIP-baserade IVRs. Funktionella scenarier för SIP session etablering och motsvarande sessionen inställningar för den föreslagna kö modell mäts. Resultaten indikerar att prototypen tjänar som en tillräcklig modell för en kö, även om en betydande fördröjning införs för sessionsupprättandebegäran. Arbetet omfattar även analys av integrering DiaStar™ är en SIP-baserad media kodkonvertering motor till denna kö. Emellertid är detta system inte helt att fungera med DiaStar för media translation. The studie avslutas med ett omnämnande av de områden för framtida arbete med detta system och det allmänna tillståndet i IVVR kö-system i branschen.
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Hitchcock, Jonathan. "Decorating Asterisk : experiments in service creation for a multi-protocol telephony environment using open source tools". Thesis, Rhodes University, 2006. http://hdl.handle.net/10962/d1006539.
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As Voice over IP becomes more prevalent, value-adds to the service will become ubiquitous. Voice over IP (VoIP) is no longer a single service application, but an array of marketable services of increasing depth, which are moving into the non-desktop market. In addition, as the range of devices being generally used increases, it will become necessary for all services, including VoIP services, to be accessible from multiple platforms and through varied interfaces. With the recent introduction and growth of the open source software PBX system named Asterisk, the possibility of achieving these goals has become more concrete. In addition to Asterisk, a number of open source systems are being developed which facilitate the development of systems that interoperate over a wide variety of platforms and through multiple interfaces. This thesis investigates Asterisk in terms of its viability to provide the depth of services that will be required in a VoIP environment, as well as a number of other open source systems in terms of what they can offer such a system. In addition, it investigates whether these services can be made available on different devices. Using various systems built as a proof-of-concept, this thesis shows that Asterisk, in conjunction with various other open source projects, such as the Twisted framework provides a concrete tool which can be used to realise flexible and protocol independent telephony solutions for a small to medium enterprise.
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Berezhnaia, Veronika. "Synthesis and characterization of helicenes, dendrimers and molecular asterisks". Thesis, Aix-Marseille, 2018. http://www.theses.fr/2018AIXM0341.
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Projet de thèse consiste de plusieurs parties:-synthèse de nanographène chirale, caractérisation de ses propriétés photo-physiques (publication en JACS acceptée);-synthèse des astérisques moléculaires, qui combinent les propriétés luminescences du noyau benzène persulfuré et des solubilité aqueuse et biocompatibilité des dendrons avec des branches de PEG; une application de ces dendrons pour couvrir les nanoparticules de silice ;-synthèse des astérisques moléculaires avec noyau de benzène persulfuré et branches des heterocycles, pour explorer leurs auto-assemblage et propriétés chelatantes vers métauxThe synthesis of molecular asterisks as "smart sensors" with a persulfurated benzene core and various ligands was explored. Asterisks with heteroaromatic ligands were synthesized for exploring their metal ions complexing ability. PEG dendrons were tested for increasing the water solubility of the asterisks, and to verify if PEG asterisks could encapsulate some hydrophobic molecules. The PEG dendrons were synthesized for making smart asterisks sensors, and for another purpose: the coating of silica nanoparticles to prevent their fast degradation in water. Unfortunately, this protection from degradation was ineffective, but we could make PEG asterisks to encapsulate molecules (Nile Red) toward new drug nanocarriers.The synthesis of chiral nanographenes embedding [5]- or [7]helicenes is reported. Usually [5]helicenes are configurationally labile, while higher helicene homologs are stable. We synthesized a stable chiral nanographene with six embedded [5]helicene units (a [5]helicene trimer). We found a strong distortion of benzene rings, especially in the triphenylene core. The enantiomers were separated and the chiroptical properties were measured.The synthesis of [7]helicene trimer was also performed with a great enantio- and diastereoselectivity. When a Ni(0)- mediated cyclotrimerization is achieved with an enantiopure [7]helicene substrate, only one enantiomer of the trimer is obtained, while keeping intact the configuration of the substrate. On a racemic substrate, three diastereomers were obtained. One of them demonstrated the highest values of a benzene ring distortion reported to date (mean puckering angle and twisting angle)
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Clayton, Bradley. "Securing media streams in an Asterisk-based environment and evaluating the resulting performance cost". Thesis, Rhodes University, 2007. http://eprints.ru.ac.za/851/.
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BORGES, FILHO Estillac Lins Maciel. "Uma ferramenta para projeto de sistemas de diálogos para call center baseados em asterisk". Universidade Federal do Pará, 2013. http://repositorio.ufpa.br/jspui/handle/2011/5615.
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Este trabalho apresenta o aplicativo DialogBuilder, uma ferramenta de código aberto escrita em Java que disponibiliza ao usuário uma interface para projeto de sistemas de diálogos e exportação destes para implantação no software Asterisk, o mais popular framework VoIP. O DialogBuilder disponibiliza um wizard para que o usuário leigo possa projetar seu sistema sem precisar aprender a programar para Asterisk. O software separa a fase de concepção do diálogo de sua codificação e se posiciona para tornar técnica e economicamente viável, mesmo para pequenas empresas, construir e manter sistemas de diálogo para aplicações telefônicas.
This work presents DialogBuilder, an open-source Java tool that provides to its user an interface for designing dialog systems and exporting them to code that can be deployed in Asterisk, the most popular VoIP framework. DialogBuilder offers to the novice users a wizard so that they can design their own dialog system without learning the intricacies of programming for Asterisk. The software separates the stages of dialog conception and its programming and is positioned to make technically and economically viable, even for small businesses, to construct and maintain dialog systems for telephony applications.
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Šalko, Jaroslav. "Implementace WebRTC v Open source PBX". Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2018. http://www.nusl.cz/ntk/nusl-377319.
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The topic of this work is verification of support WebRTC communication through selected Open Source PBX. This work examine demands for WebRTC communications and describes configuration of branch centers for this type of communication. In the theoretical part is reader acquainted with the term WebRTC and with protocols related to this kind of communications. The purpose of this part of the work is to bring the reader closer look to the principles of functioning to ensuring support for this kind of communications. This is also connected with Description of basic interfaces of WebRTC applications. Further the reader finds the configuration of the selected Open Source PBX so that they can make audio-video call between WebRTC clients. This section is divided into three subchapters, each of it deals with the same problems for one of the aforementioned PBX. At the end of each chapter where the PBX PBX is configured step-by-step, test calls are made. These calls are captured by the Wireshark packet analyzer and serve as a demonstration of the WebRTC configuration functionality. At the end of this section, PBXs are compared against each other about WebRTC support. Practical part is dealing with laboratory task for students which are studying subject telecommunication and information systems. In the task students will be configuring WebRTC for PBX Asterisk. The task contains brief description of WebRTC and comments for all steps for configuration. All steps and facts are demonstrated by exemplary configuration files.
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Gallagher, Tom. "The impact of Asterias rubens on a commercial mussel bed". Thesis, Bangor University, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.516879.
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Dubois, Philippe. "Tissu squelettique et calcification chez Asterias rubens L.(Echinodermata: Asteroidea)". Doctoral thesis, Universite Libre de Bruxelles, 1988. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/213314.
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Schildt, Holger. "VoIP mit IAX". Universitätsbibliothek Chemnitz, 2004. http://nbn-resolving.de/urn:nbn:de:swb:ch1-200400518.
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Workshop "Netz- und Service-Infrastrukturen"
Das Inter-Asterisk eXchange (IAX)-Protokoll ermöglicht eine unproblematische Kommunikation zwischen IAX-fähigen VoIP-Systemen. In der Präsentation zu dem Vortrag werden das Protokoll vorgestellt und die Vorteile von IAX skizziert.
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Sandström, Kristoffer. "Migrering till IP-baserad telefonilösning". Thesis, KTH, Skolan för informations- och kommunikationsteknik (ICT), 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-175390.
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Användandet av IP-telefoni har de senaste åren ökat och marknaden förutspås fortsätta att växa globalt. Många vill ta del av de fördelar som den nya tekniken har i form av ny funktionalitet och minskade kostnader. Men att migrera telefoni till datanät medför både nya möjligheter men också nya utmaningar. I den här rapporten undersöks hur Asterisk kan användas som ett bra IP-PBX alternativ. Rapporten behandlar även säkerheten i att ansluta ett system med Asterisk till internet genom intrusionstester som utförs på systemet i grundkonfiguration. Dessa tester resulterar i rekommendationer om hur systemet kan konfigureras för att hålla en hög säkerhetsnivå.The usage of IP-telephony has increased in recent past and the market is expected to continue to grow globally. Many want to take part in the advantages that the new technology brings in form of functionality and reduced costs. But to migrate telephony to data networks brings both new possibilities but also new challenges. This report examines how Asterisk can be used as a good IP-PBX alternative. The report also addresses the security aspect of connecting a system based on Asterisk to the internet through conducting intrusion tests on the system in standard configuration. These tests result in recommendations on how the system can be configured to keep a high security standard.
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Vassaux, Antoine. "Mécanisme de biosynthèse et production de l’astine, un pentapeptide cyclique non-ribosomique de Cyanodermella asteris". Thesis, Lille 1, 2019. http://www.theses.fr/2019LIL1R027/document.
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L’astine C est un peptide cyclique assemblé au cours d’un mécanisme nonribosomique par une synthétase dite NRPS (NonRibosomal Peptide Synthetase). Ce peptide nonribosomique possède des propriétés thérapeutiques intéressantes avec notamment des activités anti-tumorale et anti-inflammatoire. Jusqu’ici ce composé était exclusivement extrait à partir des racines d’Aster tataricus, une plante utilisée traditionnellement en médecine japonaise et chinoise. Récemment, une production d’astine C a été mise en évidence chez Cyanodermella asteris, un champignon endophyte de cette plante. Cette découverte ouvre de nouvelles perspectives en matière de production d’astine C, qui reste néanmoins très limitée en raison du faible taux de croissance du champignon endophyte. Au cours de cette étude, deux approches ont été développées parallèlement afin d’augmenter les taux de production de l’astine C. La première stratégie consistait à augmenter les rendements en optimisant la production homologue à partir de C. asteris. Dans cette optique, un système de culture a été établi afin de cultiver le champignon exclusivement sur un support en acier inoxydable. Ce mode de culture a favorisé à la fois le développement de la biomasse fongique et la production du composé d’intérêt. En vue d’optimiser ce procédé, l’impact de plusieurs paramètres de culture (modalité de préparation du support, type d’inoculum, pH de culture, et composition du milieu de culture) sur la production d’astine C a été évalué. Les paramètres de culture optimisés ont permis d’améliorer de nouveau les rendements en astine C, ce qui constitue une première étape dans le développement d’un procédé de production à l’échelle industrielle. En parallèle, des travaux ont été menés afin de développer un système de production hétérologue d’astine C chez la levure. Cette approche n’a pu être considérée qu’après avoir identifié, par le biais d’analyses bioinformatiques, les gènes impliqués dans la voie de biosynthèse de l’astine. Une fois ces gènes identifiés, une revue de la littérature a permis, entre autres, de dresser un bilan des outils moléculaires disponibles pour le clonage des larges séquences nucléiques codant pour les NRPSs, et de sélectionner des hôtes hétérologues appropriés. Des séquences complète ou partielle du gène codant pour l’astine synthétase ont été clonées respectivement chez Saccharomyces cerevisiae et Yarrowia lipolytica. Pour les deux levures considérées, une expression hétérologue a été constatée. Chez S. cerevisiae, la synthèse de la NRPS d’astine n’a pas pu être démontrée. En revanche, pour la première fois, la production d’une structure de type NRPS chez Y. lipolytica a pu être mise en évidence. Bien qu’aucun peptide nonribosomique n’ait été détecté, cette étude a permis de lever une partie des verrous limitant le développement d’un mode de production hétérologue d’astine chez la levureAstin C is a cyclic peptide assembled through a nonribosomal mechanism by a NonRibosomal Peptide Synthetase (NRPS). This nonribosomal peptide displays promising therapeutic properties including anti-tumor and anti-inflammatory activities. For decades, this compound was only extracted from the roots of Aster tataricus, a well-known plant in traditionnal japanese and chinese medicine. Recently, Cyanodermella asteris, a fungal endophyte of this plant, was demonstrated to be able to synthesize astin C. This discovery offers new opportunities for the production of this compound of interest. Nonetheless the very low growth rate of this endophytic fungus is an obstacle limiting the astin C production. In this study, two distinct approaches were conjointly considered to upscale the production rate of this compound. The first strategy was related to an optimization of the homologous production from C. asteris. For this purpose, an innovative cultivation system has been developed enabling to grow the fungus exclusively on a stainless steel support. This cultivation condition turned out to be favorable both for the fungal biomass development and for the astin C production. In order to further optimize this process, the effects of several culture parameters (i.e. support pre-treatment procedure, inoculum type, pH, medium composition) on the astin C production rates was investigated. Under optimized conditions, astin C yields were further enhanced, constituting a first step towards the development of an astin C production process at industrial scale. Meanwhile, a study was conducted in order to develop a heterologous expression system for astin C production in yeast. The identification, through bioinformatics analyses, of the genes involved in the astin biosynthesis was a precondition for the development of this approach. Once these genes have been identified, a literature review has enabled to compile the molecular tools applicable for the cloning of NRPS long lenght sequence, and to select a proper host to heterologously express them. The whole sequence or a truncated one have been transfered respectively to Saccharomyces cerevisiae or Yarrowia lipolytica. In boh considered yeasts, a heterologous expression of the foreign sequences was confirmed. In S. cerevisiae, the synthesis of the astin NRPS could not be demonstrated. On the other hand, in Y. lipolytica, for the first time, the production of a NRPS-type structure was detected. Although no nonribosomal peptide was detected, this study enabled to overcome several of the hurdles limiting the development of a astin heterologous production way in yeast
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Bořuta, Petr. "Princip hlasové komunikace v IP sítích a její bezpečnost". Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2008. http://www.nusl.cz/ntk/nusl-217548.
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This master’s thesis deals with security properties of protocols used for VoIP systems. In the first part, there is a description of most commonly used protocols and structure of VoIP systems. This part also discuss signaling and transport protocols. The second part of this paper describes techniques of ensuring quality of services. The next part presents SIP messages and communication. Last part of this paper overviews security risks of VoIP protocol. Practical part of this thesis describes creation of a testing VoIP network, on which several attacks has been made, fallowed by securing of mentioned VoIP network. Result of this thesis is evaluation of security risks connected to VoIP communication.
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Chalás, Jaroslav. "Metody zajištění bezpečnosti VoIP provozu Open source PBX". Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2010. http://www.nusl.cz/ntk/nusl-218574.
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Main goal of creating the Open Source project and GPL licence are free sources and applications available for a wide public. Competent communities are responsible for support and upgrade of Open source based applications and softwares, which are created on a voluntary bases. Due to this fact an implementation depends on plenty others publicly available libraries and applications, which sometimes complicate the installation process itself. Successfully created VoIP connection is two-phase based process. Signalization is necessary in the first place, which might be supported with H.323 or SIP. After call parameter negotiation – voice codec, cipher code, ports etc, the second phase takes over to transfer voice. Theoretical part of this thesis describes SIP, H.323, MGCP, RTP and IAX protocols, as well as secure ways of signalization and voice stream part of the call. These might be SIPS, SRTP, ZRTP and IPsec. In thesis Open Source Asterisk PBX is well described, when mentioning its options, features and community support. I put near options available for particular releases and introduce attacks and abuses which are possible to perform on the VoIP system in general, together with available, no cost and working tools to perform the attacks with. Practical part focuses on possibilities to generate experimental attacks on individual systen parts with exact definition of what the consequences are. Based on the overall analyse of achieved results I conclude three solutions as autoinstallation linux packages. These „deb“ packages consist of specific Asterisk release required to meet the security needs, ready-to-test configuration and guide to follow with correct options to set. Final security possibilities requires hardening on application layer, where Iptables takes its part. „Linux firewall“ as some express Iptables are configured to reflect VoIP system parameters and protect from DoS attacks.
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Jahn, Linda. "Characterization of a new endophytic astinproducer, Pelliciarosea asterica, from Aster tataricus". Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-190281.
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Aster tataricus (Asteraceae) is a plant native to Northern Asia and known for its use in the Traditional Chinese and Japanese Medicine. Beside many other secondary metabolites, it contains pentapeptides called astins from which some show an antitumor activity against different human cell lines. Astins are chlorinated, cyclic pentapeptides consisting of proteinogenic and non-proteinogenic amino acids. The astin structure indicates the involvement of non ribosomal peptide synthetases as well as flavin-dependent halogenases. Both enzymes are currently only known from bacteria and fungi.
A new endophytic fungus Pelliciarosea asterica was isolated from A. tataricus which produces some of the astins found in the different plant organs. The nearest neighbors of P. asterica are ostropalean fungi from the Stictidaceae lineage (Stictidaceae, Ostropales, Lecanoromycetes, Pezizomycetes, Ascomycota).
P. asterica is located in all plant organs of A. tataricus but the highest accumulation of the fungus is found in rhizomes and above-ground organs like leaves or inflorescences. In contrast, the highest astin concentration was found in the roots where nearly no fungus was detectable. P. asterica produces only one of the dichlorinated astins (astin C) in liquid culture, but in A. tataricus all three forms of the dichlorinated astins (A/B and C) were found. This indicates that either the plant is “using” the fungal astin C and metabolize it into one of the other astins or that the fungus, once living inside the plant, is itself producing the other astins.
It was also searched for a candidate gene of a halogenase which is essential for the dichlorination of the astins with an antitumor activity. No halogenase could be found by PCR or Southern as well as colony blot, neither in A. tataricus nor in P. asterica. Even the genome sequencing of P. asterica revealed no candidate gene for a halogenase.
Endophytes support the plant by suppressing pathogens (antibiosis) or by providing additional nutrients like phosphates or iron to the plant. P. asterica can solubilize different phosphate sources on agar plates. Different fungi are inhibited in growth by P. asterica on agar plates.
The endophyte P. asterica from A. tataricus supports its host in different ways and produces secondary metabolites. These secondary metabolites seem to be fungal metabolites either used or degraded by the plant. P. asterica is therefore a good alternative for a possible large-scale production of such antitumor acting astins.
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Jayasuriya, Kariyawasam Marthinna Gamage Gehan. "COMPARATIVE BIOLOGY OF SEED DORMANCY-BREAK AND GERMINATION IN CONVOLVULACEAE (ASTERIDS, SOLANALES)". UKnowledge, 2008. http://uknowledge.uky.edu/gradschool_diss/639.
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The biology of seed dormancy and germination of 46 species representing 11 of the 12 tribes in Convolvulaceae were compared in laboratory (mostly), field and greenhouse experiments. Seeds were tested for kind of dormancy and storage behavior; artificial or simulated natural treatments were applied to break physical dormancy (PY); the initial route of water entry (“water gap”) into seeds was identified; the morphoanatomy of the water gap was compared in seeds of 17 species; ontogenetical differences between water gap and seed coat away from the hilum were described in Ipomoea lacunosa seeds; cycling of sensitivity to dormancy break was elucidated in seeds of I. lacunosa, I. hederacea, Cuscuta australis and Jaquemontia ovalifolia; and mechanism of opening of the water gap was determined for seeds of I. lacunosa and of I hederacea.
Seeds of only three of the 46 species were nondormant. Two of these were recalcitrant (Maripa panamensis and Erycibe henryi), and the other one was orthodox (Bonamia menziesii). Seeds of the other 43 species were orthodox and had PY except those of Cuscuta europea, which also had physiological dormancy (PD) i.e. combinational dormancy (PY + PD). Two bulges adjacent to the micropyle were identified as the water gap in all seeds with PY except those of Cuscuta, in which the hilar fissure is the water gap. Anatomy of the bulges (water gap) adjacent to the micropyle differs from that of seed coat away from the bulges. A different sequence and phase of anticlinal and periclinal cell divisions during development created weak transitional zones between bulge - hilum and bulge - seed coat away from hilum. Water vapor pressure changes below the bulges caused formation of the opening(s) in water gap. Seeds of I. lacunosa I. hederacea, C. australis and J. ovalifolia cycle between sensitive and insensitive states to dormancy break, but not between PY and nondormancy. Seed dormancy and storage characteristics and anatomy and morphology of dormancy of seeds of Convolvulaceae closely follow the molecular phylogeny of the family. I suggest that PY in seeds of subfamily Convolvuloideae evolved from nondormant recalcitrant seeds of an ancestor closely related to Erycibeae.
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Terry, Martin Kilman. "A tale of two cacti : studies in Astrophytum asterias and Lophophora williamsii /". Diss., View online, 2005. http://repository.tamu.edu/bitstream/handle/1969.1/5023/etd-tamu-2005C-BOTN-Terry.pdf?sequence=1.
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Villa, Marco. "Smart and highly phosphorescent asterisks for (bio)sensors, antennae and molecular imaging". Thesis, Aix-Marseille, 2018. http://www.theses.fr/2018AIXM0540.
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Cette thèse porte sur des matériaux luminescents sulfurés, notamment des astérisques moléculaires persulfurés phosphorescents, basés sur l'hexakis(p-tolylthio)benzène (HTTB), et d'un thiosphérulène (balle moléculaire soufrée). Non émissif en solution, HTTB est un des émetteurs phosphorescents les plus puissants à l'état solide, avec une émission induite par agrégation (AIE). Un des objectifs est de synthétiser des dérivés portant des acides carboxyliques en périphérie pour augmenter la solubilité aqueuse et mettre à profit la coordination sélective d'ions Pb(II) et Cd(II) afin de former des édifices supramoléculaires rigides luminescents et des capteurs intelligents. Ces fonctions permettent d’ancrer les astérisques à l'intérieur de nanoparticules de silice (NPs) pour activer la phosphorescence. Ces nouvelles NPs, dont l’émission est sensible à l’oxygène, sont un colorant pour l'imagerie in vivo par microscopie à deux photons d'excitationThis thesis focuses on sulfurated luminescent materials, including phosphorescent persulfurated molecular asterisks, based on hexakis(p-tolylthio) benzene (HTTB), and a thiospherulene (a sulfurated molecular ball). Non-emissive in solution, HTTB is one of the most powerful phosphorescent emitters in the solid state, from an aggregation-induced emission (AIE). One of the objectives is to synthesize some asterisk derivatives bearing carboxylic acids at their periphery to increase their aqueous solubility, and to take advantage of the selective coordination of Pb (II) and Cd (II) ions in order to form luminescent rigid supramolecular edifices and intelligent sensors. These functions allow to anchor the asterisks inside some silica nanoparticles (NPs) to activate phosphorescence. These new NPs, whose emission is sensitive to oxygen, are dyes for in vivo imaging by two-photon excitation microscopy
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Božek, Martin. "Open IMS Core a IP Multimedia Subsystem". Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2011. http://www.nusl.cz/ntk/nusl-218969.
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This thesis describes architecture of IMS and shows possibilities of IMS platform testing. Theoretical part describes layer model of the IMS as a whole and then describes it’s individual layers. Next chapters analyse key entities of the IMS, interconnection between reference points and features of protocols used in the IMS. Practical part deals with the introduction of Open IMS Core, which was chosen for the IMS technology testing. Settings necessary to carry out testing and interconnection between PBX Asterisk are shown in next chapters. After introduction of IMS desktop clients is carried out an instant messaging communication within the IMS network. The communication is captured and analysed by Wireshark application. Afterwards there is described how SIP protocol sends messages within the IMS. After a brief introduction to the PBX Asterisk, there are discussed assumptions for the interconnection between Asterisk and IMS. There are also described necessary settings needed for implementation and communication testing itself. The first test is an audio session carried out between the desktop IMS client and IP phone registered to the PBX Asterisk. Communication is captured for the analysis of preparation, conduction and termination of the session. After the successful realization of the audio call, video session has been made. The session was analysed in detail, including statistics of control signals and transmitted packets. There are two laboratory excercises in attachement of this thesis, which will help students to understand the IMS technology and communication options within the IMS network
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Hýbner, Lukáš. "Využití SIP serveru na FIT pro IP telefonii". Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2008. http://www.nusl.cz/ntk/nusl-235882.
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Master's Thesis is engaged on possibilities connect SIP server to telephone network on FIT. The main reason is that employees can call to university, when they are out of faculty. For resolution we will use SIP server Asterisk, which will be serving as authorization server for users. Next Asterisk will ensure transmission numbers to SIP address with ENUM. In the practical part we will verify the functionality.
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Chombard, Catherine. "Les demospongiae à asters : phylogénie moléculaire et homologie morphologique". Paris, Muséum national d'histoire naturelle, 1998. http://www.theses.fr/1998MNHN0025.
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Avec le développement de la systématique moléculaire, il est maintenant possible de tester a posteriori des hypothèses sur l'homologie morphologique et les hypothèses évolutives qui en découlent. Les phylogénies moléculaires permettent de tester la classification. En positionnant les caractères non moléculaires sur ces phylogénies, il est donc possible d'inférer leur schéma évolutif et donc de réévaluer leur utilisation en systématique. La systématique des spongiaires s'est révélée particulièrement difficile de ses débuts, car ces organismes ne présentent qu'un faible nombre de caractères morphologiques et les hypothèses évolutives publiées depuis la fin du XIXème siècle sont presque entièrement subjectives. La systématique des spongiaires n'est donc pas encore phylogénétique. Une quarantaine d'espèces de demospongiae ont été séquencées pour un fragment de l'ADNr 28s. L’échantillonnage a été centre sur les demospongiae présentant le type spiculaire aster, la plupart appartenant soit à l'ordre des astrophorida, soit à celui des hadromerida. Ces taxons sont relativement riches en caractères morphologiques et certains de ces caractères ont pu être réévalues d'après les données moléculaires. À l'intérieur de l'ordre des astrophorida, les résultats moléculaires sont en contradiction avec la classification familiale et ne permettent pas de soutenir l'hypothèse de deux sous-ordres monophylétiques distingues par la présence exclusive de microscleres de type streptosclere et euaster respectivement. L’étude des Hadromerida montre en revanche une très bonne corrélation entre phylogénie moléculaire, systématique familiale et répartition des types d'asters. Trois genres de demospongiae a asters ne montrant aucune affinité avec les ordres étudies ci-dessus, ont nécessité d'être replaces dans une phylogénie générale des demospongiae. L’analyse de l'ensemble des séquences d'ARNr 28s disponibles pour les diploblastiques laisse supposer une émergence très ancienne de cinq groupes distincts de demospongiae, deux de ces groupes constituant des assemblages entièrement nouveaux et contenant chacun des demospongiae a asters. Ces résultats posent probablement les bases d'une future révolution dans la classification des demospongiae.
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Li, Zhang. "Service Improvements for a VoIP Provider". Thesis, KTH, Kommunikationssystem, CoS, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-91493.
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This thesis project is on helping a Voice over Internet Protocol (VoIP) service provider by improving server side of Opticall AB's Dial over Data solution. Nowadays, VoIP is becoming more and more popular. People use VoIP to call their family and friends every day. It is cheap, especially when users are abroad, because that they do need to pay any roaming fee. Many companies also like their employees to use VoIP, not only because the cost of calling is cheap, but using VoIP means that the company does not need a hardware Private Branch eXchange (PBX) -- while potentially offering all of the same types of services that such a PBX would have offered. As a result the company can replace their hardware PBX with a powerful PC which has Private Branch eXchange PBX software to connect all the employees and their VoIP provider. At the VoIP provider’s side, the provider can provide cheap calls for all users which are connected by Internet. The users can initialize and tear down a session using a VoIP user agent, but how can they place a VoIP call from a mobile device or other devices without a VoIP user agent? Users want to place cheap VoIP call everywhere. VoIP providers want to provide flexible solution to attract and keep users. So they both want to the users to be able to place cheap VoIP call everywhere. Although VoIP user agent are available for many devices as a software running on a computer, a hardware VoIP phone, and even in some mobile devices. However, there are some practical problems with placing a VoIP call from everywhere. The first problem is that not every device can have a VoIP user agent. But if you do not have a VoIP user agent on your device, then it would seem to be difficult to place a VoIP call. The second problem is that you have to connect to a network (probably Internet) to signal that you want to place a call. Thus at a minimum your device has to support connecting to an appropriate network. If your device is connecting to a mobile network, you can send signaling to set up a VoIP call through General Packet Radio Service (GPRS). However, the bandwidth and delay of the GPRS networks of some mobile operators is not suitable for the transfer of encoded voice data, additionally, some mobile operators charge high fees for using GPRS. All of these problems make placing VoIP calls via a mobile device difficult. However, if your mobile device has a VoIP user agent and you have suitable connectivity, then you can easily use VoIP from your mobile device[.] To provide a flexible solution to VoIP everywhere -- even to devices that do not or can not have a VoIP user agent, Opticall AB has designed Dial over Data (DoD) solution. By using this solution, you can place a VoIP call from your mobile device or even fixed phone -- without requiring that the device that you use have a VoIP user agent. This solution also provides a central Internet Protocol-Private Branch eXchange (IP-PBX) which can connect call to and from to Session Initiation Protocol (SIP) phones. Both individuals and companies can use this solution for call cost savings. Max Weltz created the existing DoD solution in an earlier thesis project. This thesis [1] provides a good description of the existing DoD solution. As a result of continued testing and user feedback, Opticall AB has realized that their DoD solution needs to be improved in several area. This thesis project first identified some of the places where improvement was needed, explains why these improvements are necessary, and finally designs, implements, and evaluates these changes to confirm that they are improvements. An important result of this thesis project was a clear demonstration of improvements in configuration of the solution, better presentation of call data records, correct presentation of caller ID, and the ability to use a number of different graphical user interfaces with the improve DoD solution. These improvements should make this solution more attractive to the persons who have to maintain and operate the solution.Detta examensarbete behandlar förbättringar i serversidan av OptiCall ABs “Dial over Data” (DoD) lösning som tillhandahålls för tjänsteleverantörer av VoIP. VoIP blir mer och mer populärt. Människor använder VoIP för att ringa till sin familj och vänner varje dag. Det är billigt, särskilt när användaren är utomlands, eftersom de inte behöver betala någon roamingavgift. Många företag vill också att deras anställda skall använda IP-telefoni, inte bara därför att kostnaden för att ringa oftast är lägre, utan för att bolaget kan ersätta sin traditionella företagsväxel (PBX) med en kraftfull dator som har PBX programvara för att även ansluta alla anställda till deras VoIP leverantör. VoIP leverantören kan erbjuda billiga samtal till alla användare som också är anslutna via Internet. Användarna kan hantera VoIP samtal med en VoIP user agent, men hur kan de ringa ett VoIP-samtal från en mobil enhet eller andra enheter utan VoIP user agent? Användare vill kunna ringa billiga VoIP-samtal överallt. VoIP-leverantörer vill erbjuda en flexibel lösning för att locka och behålla användare. Även VoIP user agent finns utvecklade till många enheter som en programvara som körs på en dator, en hårdvara VoIP-telefon, och även i vissa mobila enheter. Men det finns vissa praktiska problem med att ringa ett VoIP-samtal från alla platser. Det första problemet är att inte varje enhet kan ha en VoIP user agent. Det andra problemet är att den måste ansluta till ett nätverk (troligen Internet) för att signalera att den vill ringa ett samtal. Om din enhet ansluter till ett mobilnät, kan du skicka signalerar att upprätta ett VoIP-samtal via General Packet Radio Service (GPRS). Dock är bandbredden och fördröjningen i GPRS-nät i vissa operatörers nät inte lämpliga för överföring av tal, dessutom tar vissa mobiloperatörer ut höga avgifter för att använda GPRS. Alla dessa problem gör det svårt att hantera VoIP-samtal via en mobil enhet. Men om din mobila enhet har en VoIP user agent och du har lämplig nätanslutning så kan du enkelt använda VoIP från din mobiltelefon[.] För att erbjuda en flexibel VoIP lösning överallt - även på enheter som inte kan ha en VoIP user agent har OptiCall AB utformad “Dial over Data” (DoD). Genom att använda denna lösning kan du initiera ett VoIP-samtal från din mobiltelefon eller fast telefon - utan att kräva att den enhet som du använder har en VoIP user agent. Denna lösning inkluderar också en central Internet Protocol-Private Branch Exchange (IP-PBX) som kan koppla samtal till och från Session Initiation Protocol (SIP) telefoner. Både privatpersoner och företag kan använda denna lösning för att minska samtalskostnader. Max Weltz vidareutvecklade den befintliga DoD lösning i ett tidigare examensarbete. Denna avhandling [1] ger en god beskrivning av den befintliga DoD lösning. Som ett resultat av fortsatt testning samt synpunkter från användarna har OptiCall AB insett att deras DoD lösning måste förbättras på flera områden. Detta examensarbete har i första hand identifierat några områden där förbättringar behövdes, förklarat varför dessa förbättringar är nödvändiga, och slutligen utvecklat och utvärderat dessa förändringar. Ett viktigt resultat av detta examensarbete visades av en tydlig demonstration av förbättrad utformning av lösningen. Gränssnittet fick bla en bättre presentation av samtalshistorik, mer korrekt nummerpresentation. Dessa förbättringar bör göra denna lösning mer attraktivt för de personer som skall använda och underhålla lösningen.
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Weltz, Max. "Dial over Data solution". Thesis, KTH, Kommunikationssystem, CoS, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-91874.
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The increased use of computer networks has lead to the adoption of Internet-based solutions for reducing telephony costs. This has proved to be a boon to callers who can reach the other party directly via the Internet. Unfortunately numerous business persons still need to call to and from mobile phones which are currently a domain where the customers are generally tightly bound to their operators. To provide a simple solution to this problem for companies, Opticall AB has designed an integrated system called the Dial over Data solution, coupling a mobile interface with a low-rate communication channel, which allows calls to be originated remotely at the best price, exploiting the customer company's existing network. This scheme allows the customer company to easily control telecommunications costs, to monitor their employees' efficiency, and more generally speaking to claim a central role in the communications of their employees. The proposed solution allows distant callers (usually employees of the customer company) to benefit from the company's internal network, which is usually more cost effective and offering connectivity to more networks than a cell phone. The Dial over Data solution enables communication between any phone accessible from the customer company's telephony network (such as SIP clients, landline phones, and mobile phones) at a lower cost.</p> This thesis project analyzes existing technologies and compares them to the pre-existing prototype to ascertain the validity of the method and of the components used. This project also explains the improvements brought to the features offered by the DoD solution: the initial prototype has been developed into a stable and functional product, and has been tested internally. Prompted by a need for scalability and additional features, the replacement of Asterisk for the handling of SIP calls by other SIP servers has also been considered and tested.Den nuvarande ökningen av datanätverk har lett till adoptionen av Internetbaserade lösningar för att förminskar kostnader inom telefoni. Tyvärr behöver åtskilliga affärsmän fortfarande ringa till och ifrån mobiler som återstår som ett område där kunderna är fastkedjade till deras operatörer. För att tillföra en enkel lösning till detta problem för kundföretag har Opticall AB planlagt ett integrerat system som kallas Dial over Data som kopplar ihop ett mobilt gränssnitt, med en billig kommunikationsmedel, som tillåter telefonsamtal påbörjas avlägset på det billigaste priset tack vare företagets nätverk. Det ger möjligheten till kundföretaget att vara centralt för sina personals kommunikationer. Det medger ett enkelt sätt att kontrollera kostnader samt övervaka personalens effektivitet. Den Dial over Data lösningen är en lösning som tillåter avlägsen besökarna med kundföretagets inre nätverk kommer att dra nytta av eftersom det är mer kostnadseffektiv och flexibel än en blott mobiltelefon. Denna möjliggör kommunikation mellan SIP-klienter, fast telefoni och mobiltelefoni för en lägre kostnad till företaget utan att framkalla besvär för sina anställda. Konnektiviteten till företagets inre nätverk samt en låg besvärlighetsnivå är garanterade respektive genom konfigurationsförmågan av produkten och ett praktiskt gränssnitt som är redo för korporativkontaktlistor och visar alla informationen som är relevanta till förbrukarnas erfarenhet.</p> Det här avhandlingsprojektet analyserar existerande teknologier och sätter dem i relation till den sedan tidigare framtagna prototypen för att utröna validiteten hos metoden och beståndsdelar. Projektet förklarar även de förbättringar som gjorts till de egenskaper som erbjuds av DOD-lösningen: prototypen har utvecklats till en stabil och funktionell produkt och har testats internt. Driven av behovet för skalabilitet och ytterligare egenskaper har ersättandet av Asterisk för hantering av SIP samtal av andra SIP servers övervägts och testats.
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Cavalcante, Anselmo de Vasconcelos. "AN?LISE DOS EFEITOS DE CODECS DE ?UDIO NA AVALIA??O DE DESVIOS VOCAIS". reponame:Repositório Institucional do IFPB, 2018. http://repositorio.ifpb.edu.br/jspui/handle/177683/344.
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Este trabalho apresenta um estudo sobre as implica??es no uso de diferentes codecs de ?udio na an?lise perceptiva e ac?stica da voz. Um cen?rio de transmiss?o baseado em VoIP foi criado, empregando o Asterisk e o softphone Microsip, para auxiliar no diagn?stico de desvios vocais ? dist?ncia. Para este prop?sito, foram utilizados 36 sinais de vozes sintetizadas e 36 sinais de vozes reais, classificados como normais, com o desvio rugosidade e com o desvio soprosidade. Cada sinal foi submetido a seis transmiss?es, cada uma delas utilizando um codec espec?fico (G.711 Lei A, Speex32, GSM Full Rate, LPCM16, Opus24 e SILK16). Antes e ap?s cada transmiss?o, um especialista em voz realizou a classifica??o dos sinais quanto ao tipo de desvio e, com aux?lio do software VoxMetria, extraiu-se as medidas ac?sticas frequ?ncia fundamental, jitter, shimmer, GNE e desvio padr?o da frequ?ncia fundamental. Observou-se que, dentre os codecs analisados, o Opus24 foi aquele se mostrou o mais promissor para avalia??o da qualidade vocal, por ter sido o ?nico a apresentar taxa de acur?cia acima de 70%, tanto para os sinais sintetizados quanto para os sinais reais analisados. Este codec conseguiu manter os desvios vocais inalterados para avalia??o em mais de 85% dos sinais reais.
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Krieger-Knieja, Jolanta. "Paraverbale Ausdrücke als Gestaltungsmittel der Textsorte Comic am Beispiel der Reihe Asterix /". Lublin : Lubelskie Towarzystwo Nauwoke, 2003. http://catalogue.bnf.fr/ark:/12148/cb39265632p.
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Silva, Adriano Clayton da 1978. "A tradução de imagens e palavras nas histórias em quadrinhos de Asterix". [s.n.], 2015. http://repositorio.unicamp.br/jspui/handle/REPOSIP/269514.
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Orientador: Maria Viviane do Amaral VerasDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Estudos da Linguagem
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Resumo: Ainda há poucos trabalhos acadêmicos explorando as possibilidades da tradução em ambientes multissemióticos como as Histórias em Quadrinhos (HQs), embora estas sejam cada vez mais consumidas, o que inclui as traduzidas. Nas HQs, as imagens, como as palavras, têm significados diferentes em cada sociedade e, por esse motivo, precisam ser consideradas na tradução. Imagens, palavras e elementos quadrinísticos (EQ) ¿ como o balão ou a onomatopeia ¿ representam um grande desafio ao tradutor, especialmente quando as imagens e os EQ não podem ser modificados por questões de direitos autorais. Esta dissertação pesquisou a tradução nas HQs, tendo como objetivo entender como os elementos não verbais afetam as escolhas do tradutor. A metodologia utilizada foi qualitativa, documental, descritiva e interpretativa, ou seja, dependente da sensibilidade e interpretação do pesquisador. O corpus compreendeu quatro álbuns impressos de HQ do personagem francês Asterix, com suas versões na língua original e as respectivas traduções para o público brasileiro. A pesquisa mostrou-se profícua e proveitosa, pois exigiu, além da fundamentação teórica no campo dos estudos de Tradução, a busca por outras teorias nos campos das Artes, da Linguística Aplicada, da Semiótica entre outros. Especial destaque à Multimodalidade e à Paratradução, ferramentas teóricas que permitem repensar as imagens e a tradução. Como resultado, concluiu-se que as imagens e os EQ influenciam muito as escolhas do tradutor, pois seu trabalho e liberdade ficam restritos ao interior dos balões, ao título, às notas de rodapé e a outros raros locais dentro de um quadrinho. Mais do que restrita, a tradução tem de se encaixar em espaços originalmente não previstos para ela, e ainda deve estar em "harmonia" com o que as imagens e EQs "dizem" em cada vinheta e no contexto geral do álbum, tira, cartum ou qualquer um dos gêneros possíveis do supergênero HQ. O tradutor então deve conhecer não apenas as línguas/culturas de chegada e de partida, mas também os elementos imagéticos, e outros sistemas semióticos e simbólicos, que afetam os diversos contextos sociais que vão surgindo na interação entre imagens, EQs e palavras
Abstract: There are few academic papers exploring the possibilities of the translation in multisemiotic environments, like the Comics, although the consumption of them is increasing, including the translated ones. In the comics, the images, as the words, have different meanings in each society and, therefore, need to be considered in translation. Images, words and exclusive comic elements (ECE) - as the balloon or onomatopoeia - represent a major challenge to the translator, especially when the images and the ECE cannot be modified by copyright issues. The aim of this study was to understand how non-verbal elements affect the translator's choices. The methodology was qualitative, documentary, descriptive and interpretive, i.e. dependent on the sensibility and interpretation of the researcher. Data analysis was conducted using four printed comic albums of the French character Asterix, with their versions in the original language and their translations for the Brazilian public. The research has been proved fruitful and profitable because it required, in addition to the theoretical foundation in the field of translation studies, the search for other theories in the fields of Arts, Applied Linguistics, Semiotics and others. Special emphasis was given to Multimodality and Paratranslation, theoretical tools that allow rethinking of the images and translations. As a result, it was concluded that the images and ECE really influence the translator's choices, since their work and freedom are limited to places such as interior of balloons, the title, the footnotes and other rare places in a frame. More than limited, the translation has to fit into spaces originally not planned for it. And it must be in "harmony" with the images and what the ECE are "saying" in each frame and in the general context of the album, strip, cartoon or any of the possible genres of the Comic Supergenre. The translator, therefore, must not only know the source/target languages and cultures, but must also know the pictorial elements and other semiotic and symbolic systems that affect the numerous social contexts that arise in the interplay between images, ECE and words
Mestrado
Linguagem e Sociedade
Mestre em Linguística Aplicada
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Santiago, Gisele. "Nas cultivares de batata asterix e atlantic por marcadores morfológicos e microssatélites". Universidade Federal de Santa Maria, 2011. http://repositorio.ufsm.br/handle/1/3198.
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Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorThe potato (Solanum tuberosum L.), Solanaceae, ranked fourth in quantity food production, exceeded only by wheat, rice and corn. The potato crop is propagated vegetative form, which requires high quality plant for income maintenance in the field. However, culture is susceptible to diseases, mainly viruses so it is necessary to use techniques such as culture of shoot tips for cleaning and subsequent clonal propagation in a system that maintains the quality of plant propagation material such as hydroponics. However, it is known that tissue culture induced genetic variability in micropropagated material. Changes collectively termed somaclonal variation. As a result, strategies are needed to control and monitor this phenomenon in potato cultivars due to the possibility of loss of genetic traits for which have been improved. Given the above, this study aimed to evaluate the in vitro behavior of ramets, evaluate characteristics of the tubers produced hydroponically, evaluate the phenotypic stability of ramets from tissue culture through 26 minimum description of potato compare the phenotypic stability of ramets of each cultivar according to the degree of differentiation of the explants that originated ramets, indicating potential minimum descriptors to monitor the phenotypic stability, to analyze the variability over the subcultures of the growing season and the field through microsatellite markers from potato cultivar Asterix and Atlantic. The results showed that ramets both of Asterix as Atlantic differ on in vitro behavior for the variables. There is variability in characteristics of tuber-seed in the ramets of potato cultivars Atlantic and Asterix, from the cultivation of basic generation zero (G0) in a closed hydroponic system. Ramets of Asterix have a different behavior of ramets Atlantic in relation to minimum descriptors. There is great variability and intraclonal divergence in relation to the phenotypic varieties in minimum descriptors in ramets of Asterix and Atlantic. The degree of differentiation of explants that form these ramets has no effect on the phenotypic stability of Asterix and Atlantic. Growth habit and pigmentation of the main stem descriptors can be useful in monitoring the genetic purity of Asterix. Growth habit, vegetative and Tuber shape have potential use in monitoring the intraclonal variability in Atlantic. The potato cultivars Asterix and Atlantic show high variability in intraclonal microsatellite markers. The pattern of instability in molecular phenotypes is different among potato cultivars Asterix and Atlantic. Ramets regenerated from shoot tip derived and indirect organogenesis are also unstable. The subculture time, alone, seems to have no effect on the incidence of somaclonal variation in potato cultivar Asterix and Atlantic.
A batata (Solanum tuberosum L.) ocupa o quarto lugar em nível mundial em produção, sendo superada apenas pelo trigo, arroz e milho. A cultura da batata é propagada de forma vegetativa e, devido à suscetibilidade a doenças, é imprescindível o emprego da cultura de ápices caulinares para efetuar limpeza clonal e, na sequência, da micropropagação das partes aéreas para a obtenção de mudas livres de patógenos para satisfazer a demanda dos agricultores. Entretanto, a cultura de tecidos pode induzir variabilidade genética no material micropropagado, a qual é denominada variação somaclonal. Face ao exposto, o objetivo geral do presente estudo consistiu na análise da variação somaclonal nas cultivares de batata Asterix e Atlantic, com o intuito de contribuir para uma melhor compreensão e, também, para o desenvolvimento de estratégias direcionadas ao controle e monitoramento desse fenômeno, e, simultaneamente, para a obtenção de variantes de utilidade para programas de melhoramento genético. Com esta finalidade, as diferentes etapas do sistema de produção de batata-semente foram avaliadas por descritores morfológicos, morfoagronômicos e marcadores microssatélites. Os resultados revelaram que os rametes de Asterix e Atlantic apresentam extensiva variabilidade intraclonal, observando-se padrões diferenciados nas duas cultivares em resposta às fontes de variação estudadas. Há variabilidade intraclonal na estabilidade fenotípica in vitro e em características de tubérculo-semente nos rametes das cultivares de batata Atlantic e Asterix, provenientes do cultivo de plantas básicas da geração inicial (G0) em sistema hidropônico. Plantas e tubérculos de primeira geração clonal apresentam grande variabilidade intraclonal e divergência em relação ao padrão fenotípico das cultivares nos descritores mínimos nos rametes de Asterix e Atlantic. O grau de diferenciação dos explantes que originam os rametes não tem efeito na estabilidade fenotípica de Asterix e Atlantic. Os rametes das duas cultivares apresentam elevada variabilidade genética intraclonal em marcadores microssatélites. O padrão de instabilidade nos fenótipos moleculares é diferenciado em Asterix e Atlantic. Rametes regenerados a partir de ápices caulinares e derivados de organogênese indireta são igualmente instáveis em nível molecular. O tempo de subcultivo pode ser usado como ferramenta de controle da variação somaclonal nas cultivares de batata Asterix e Atlantic, pela análise dos fenótipos moleculares.
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Meaders, Johnathan Lee. "Growth, Morphology, and Positioning of Microtubule Asters in Large Zygotes:". Thesis, Boston College, 2020. http://hdl.handle.net/2345/bc-ir:109018.
Texto completoResumen
Thesis advisor: David R. BurgessMicrotubule (MT) asters are radial arrays of MTs nucleated from a microtubule organizingcenter (MTOC) such as the centrosome. Within many cell types, which display highly diverse size and shape, MT asters orchestrate spatial positioning of organelles to ensure proper cellular function throughout the cell cycle and development. Therefore, asters have adopted a wide variety of sizes and morphologies, which are directly affects how they migrate and position within the cell. In large cells, for example during embryonic development, asters growth to sizes on the scales of hundreds of microns to millimeters. Due to this relatively enormous size scale, it is widely accepted that MT asters migrate primarily through pulling mechanisms driven by dynein located in the cytoplasm and/or the cell cortex. Moreover, prior to this dissertation, significant contributions from pushing forces as a result of aster growth and expansion against the cell cortex have not been detected in large cells. Here we have reinvestigated sperm aster growth, morphology, and positioning of MT asters using the large interphase sperm aster of the sea urchin zygote, which is historically a powerful system due to long range migration of the sperm aster to the geometric cell center following fertilization. First, through live-cell quantification of sperm aster growth and geometry, chemical manipulation of aster geometry, inhibition of dynein, and targeted chemical ablation, we show that the sperm aster migrates to the zygote center predominantly through a pushing-based mechanism that appears to largely independent of proposed pulling models. Second, we investigate the fundamental principles for how sperm aster size is determined during growth and centration. By physically manipulating egg size, we obtain samples of eggs displaying a wide range of diameters, all of which are at identical developmental stages. Using live-cell and fluorescence microscopy, we find strong preliminary evidence that aster diameter and migration rates show a direct, linear scaling to cell diameter. Finally, we hypothesize that a collective growth model for aster growth, or centrosome independent MT nucleation, may explain how the sperm aster of large sea urchin zygotes overcomes the proposed physical limitations of a pushing mechanism during large aster positioning. By applying two methods of super resolution microscopy, we find support for this collective growth model in the form of MT branching. Together, we present a model in which growth of astral MTs, potentially through a collective growth model, pushes the sperm aster to the zygote center
Thesis (PhD) — Boston College, 2020
Submitted to: Boston College. Graduate School of Arts and Sciences
Discipline: Biology