Tesis sobre el tema "Beef cattle Kikuyu grass"

Thesis (Ph. D.)--West Virginia University, 2007.
Title from document title page. Document formatted into pages; contains vi, 180 p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 154-169).

Flavor lexicons are used in sensory evaluation to determine the flavor profile of a food product. The objective of this study was to develop a flavor lexicon for cooked beef, which can then be used in various projects relating to beef quality such as studies investigating animal diet, marinating, ageing, or other enhancements. A descriptive panel of 10 people was used to develop a flavor lexicon of 18 attributes, including astringent, barny, bloody, brothy, browned, gamey, grassy, juicy, fatty, livery, metallic, oxidized, roast beef, and the five basic tastes (bitter, salty, sour, sweet, and umami). In contrast to other studies on beef, this lexicon was developed to include both positive and negative attributes. The lexicon was able to show that rib eye steaks from the Longissimus dorsi muscle in grass-fed animals were significantly (p<0.05) higher in barny, bitter, gamey, and grassy flavors, and lower in juicy and umami flavors. The steaks were also rated by consumers, who showed a preference for grain-fed beef over grass-fed beef. The ratings of the descriptive panel were related to the consumer panel scores to equate the lexicon terms with a positive or negative consumer degree of liking score. Those terms that were considered positive in this study due to their positive correlation with consumer liking include brothy, umami, roast beef, juicy, browned, fatty, and salty. The terms that were inversely associated with consumer liking were barny, bitter, gamey and grassy, among others. A separate descriptive panel was conducted on the Spinalis dorsi (or “cap” muscle) of the rib eye steak, with similar results. Additionally, descriptive and consumer evaluations found no difference between two types of grass diets, namely alfalfa and sainfoin. Different mixtures of beef and chicken were also evaluated to determine flavor differences between the two meats. Chicken was found to be more closely correlated to brothy, juicy, sweet, and umami, among others, while beef was found to be more closely correlated to terms such as gamey, bloody, oxidized, metallic, roast beef, and astringent. Throughout these tests, the newly developed lexicon was shown to be an effective tool for profiling fresh meat samples.

Two trials were conducted simultaneously to evaluate the effects of energy supplementation for cattle grazing tropical pastures managed with different initial sward heights on DMI and ruminal fermentation of cattle grazing intensively managed tropical grass during the rainy season. Eight 24-month-old rumen-cannulated Nellore steers were used per trial (Trial 1: 300 kg BW ± 5.97, Trial 2: 343 kg BW ± 7.40) allocated in two 4x4 Latin squares. Treatments corresponded to 0 (mineral supplementation) and a 0.3 (0.3% of BW of ground corn as fed basis) combined with 2 pre-grazing sward heights (25 and 35 cm). The stubble height was 15 cm. In the second trial the level of supplementation was 0.6% BW of ground corn as fed basis. Steers were managed in 2 ha of Palisadegrass pasture (Brachiaria brizantha marandu). Pastures were fertilized with 120 kg nitrogen/ha and averaged 13.8 and 11.0% CP and 58.8 and 63.4% NDF, for 25 and 35 cm, respectively The forage and the diet DM and CP digestibility were greater (P<0.05) for 25 then for 35 cm grazing management. For both trials 1 and 2, cattle grazing the pastures with 25 cm initial sward height consumed more forage DMI, more total DMI and more energy (P<0.05) and at the same time steers spent less time grazing (P<0.05) and more time resting (P<0.05), presented greater bite rates (P<0.05), less steps per day and less steps between feeding stations (P<0.05), when compared with cattle grazing the 35 cm pastures. Rumen pH values were less (P<0.05 in trial 1; P<0.1 in trial 2) and concentrations of rumen N-NH3 and retention of N were greater (P<0.05) for cattle grazing the 25 cm pastures while rumen VFA and microbial synthesis were not affected (P>0.05) by pasture management. Supplementing energy at 0.3% (trial 1) increased (P<0.05) diet DM digestibility while feeding energy at 0.6% (trial 2) decreased forage CP digestibility, increased (P<0.05) forage NDF digestibility and increased diet DM (P<0.05) and diet NDF (P<0.1) digestibility. Supplementing energy at 0.3% (trial 1) or at 0.6% (trial 2) decreased forage DMI (P<0.05) and substitution rates were 1.63 and 0.72, respectively. The total DMI and energy intake were not increased (P>0.05) by supplementing energy at 0.3% while increasing energy supplementation to 0.6% was effective to increase total DMI and energy intake of cattle grazing tropical forage, independent of initial sward height. Energy supplementation decreased (P<0.05) grazing time, but it did not affect (P>0.05) any other grazing behavior parameter. Supplementing grazing cattle with 0.3% had no effect (P>0.05) on rumen pH, N retention and microbial synthesis, increased (P<0.05) rumen propionate and decreased (P<0.05) rumen N-NH3, rumen acetate and acetate:propionate ratio. Supplementing grazing cattle with 0.6% decreased (P<0.05) rumen pH, rumen N-NH3, rumen acetate and acetate:propionate ratio, while it increased (P<0.05) rumen propionate, N retention and microbial synthesis. Plasma glucose was not affected by treatments (P>0.05). The pre-grazing sward height of 25 cm and feeding energy supplement at 0.6% of BW were efficient strategies to increase energy intake of cattle grazing Palisadegrass.
Dois experimentos foram conduzidos simultaneamente, para avaliar o uso da suplementação energética para bovinos manejados em diferentes alturas de entrada na pastagem. Foram usados 8 novilhos Nelore canulados no rumen por experimento (Exp. 1: 300 kg de PC ± 5,97, Exp. 2: 343 kg PC ± 7,40) distribuídos em 2 quadrados latinos 4x4. Os tratamentos para o Exp. 1 foram 0 (suplementação mineral) e 0,3 (0,3% do PC em milho moído) combinados com 2 alturas de entrada (25 e 35 cm). A altura de saída foi 15 cm. No Exp. 2 o nível de suplementação foi 0,6% do PC em milho moído. Os animais foram manejados em 2 ha de Capim Marandu, os quais foram adubados com 120 kg de N/há, apresentando valores médios de 13,8 e 11,0% de PB e 58,8 e 63,4% de FDN para pastos de 25 and 35 cm, respectivamente. A DMS e DPB da forragem e da dieta foram maiores (P<0,05) para o manejo da pastagem de 25 cm do que 35. Em ambos os experimentos, o CMS de forragem, energia e total foi maior (P<0,05) para o tratamento de 25 cm, que ao mesmo tempo promoveu menor tempo de pastejo (P<0,05), maior tempo em ócio (P<0,05) e taxa de bocado (P<0,05), menor número de passos por dia e passos entre estações de pastejo (P<0,05), quando comparados com animais mantidos no tratamento de 35 cm. O pH ruminal foi menor (P<0,05 no Exp. 1; P<0,10 no Exp. 2), a N-NH3 ruminal e retenção do N foram maiores (P<0,05) para animais manejados na altura de entrada de 25 cm. Os AGVs e a síntese microbiana não foram afetados (P>0,05) pelo manejo da pastagem. A suplementação em 0,3% (Exp. 1) aumentou (P<0,05) a DMS da dieta, enquanto a suplementação de 0,6% (Exp. 2) reduziu a DPB da forragem (P<0,05), aumentou a digestibilidade da FDN da forragem (P<0,05) e a DMS (P<0,05) e da FDN da dieta (P<0,01). A suplementação em 0,3% (Exp. 1) ou em 0,6% (Exp. 2) reduziu o CMS de foragem (P<0,05) e as taxas de substituição foram 1.63 and 0.72, respectivamente. O CMS total e de energia não foram aumentados (P>0,05) pela suplementação em 0,3%, enquanto o nível de 0,6% foi efetivo em aumentar o CMS total e de energia de bovinos mantidos em pastagem tropical, independente do manejo da pastagem. A suplementação reduziu o tempo de pastejo (P<0,05). Animais suplementados com 0,3% não alteraram (P>0,05) o pH ruminal, a retenção de N e síntese microbia, mas aumentaram (P<0,05) propionato no rumen e diminuíram (P<0,05) N-NH3 ruminal, acetato e relação acetato:propionato. A suplementação com 0,6% diminuiu (P<0,05) o pH ruminal, N-NH3 ruminal, acetato e relação acetato:propionato no rumen, aumentaram (P<0,05) o propionato no rumen, a retenção de N e a síntese microbiana. A glicose plasmática não foi alterada (P>0,05). A altura de pré-pastejo de 25 cm e a suplementação energética de 0,6% do PC foram estratégias eficientes para aumentar o consumo de energia de bovinos mantidos em pastagens de Capim Marandu.

This study investigated the hypothesis that cattle fed to produce a lean carcass during a winter restriction period will subsequently grow faster and remain leaner than fatter steers during the summer, exploiting cheap grazed grass. Three studies were carried out; in the first study steers were restricted at three levels of growth (300, 600 and 900 g/day) during winter and turned out to graze grass during summer. At each level of growth during winter steers were fed on one of two diets aimed at producing steers of either a lean or fat carcass composition. At the end of the winter restriction period differences in lean composition were observed at the 300 and 600 g/day growth rates. Steers were slaughtered when they attained the target slaughter weight at the end of summer; but there was no difference between treatments in meat quality characteristics or fat composition. Liveweight gain during the summer period was inversely correlated to winter liveweight gain. Two further studies were carried out to assess the effect of altering carcass composition during winter, fed to a predicted growth rate of 600g/day to investigate the underlying physiological and endocrinological mechanism regulating the growth characteristics. In the first study at the end of winter steers that were fatter had higher glucose and insulin concentrations. No differences between diet treatments were detected in muscle protein synthesis or breakdown. At the end of the winter in the second experiment there were no differences in carcass composition, metabolite or hormone profiles between treatments. For both studies, at the end of summer, steers on different dietary treatments had similar carcass compositions and metabolic parameters. It was concluded that by altering the diet of steers during a winter restriction period the composition of carcass gain can be manipulated. Since steers altered the deposition of carcass protein and fat during the subsequent summer period at grass resulting in similar body compositions at slaughter it was deduced the that composition of steers at the end of winter remains unimportant. Reductions in beef production costs may be attained by restricting the growth rates during winter when commercial feeds are expensive.

O presente trabalho teve por objetivo avaliar o efeito do teor de matéria seca (MS), do tamanho de partícula e da adição de inoculante bacteriano sobre as características químico-físicas e perdas no processo de conservação da silagem de capim Tanzânia (Panicum maximum, Jacq. cv. Tanzânia), assim como avaliou o comportamento ingestivo, digestão de nutrientes e o desempenho em bovinos de corte. No experimento I analisou-se o efeito dos tratamentos sobre a fermentação e as perdas na ensilagem. Os índices de recuperação de matéria seca (RMS) e as perdas na forma de efluente e gases foram de 90,58%; 53,66 kg.t MV-1 e 6,40% da MS nas silagens nãoemurchecidas, 93,62%; 16,81 kg.t MV-1 e 4,97% em silagens adicionadas com milheto grão e 92,19%; 3,55 kg.t MV-1 e 6,16% nas silagens emurchecidas, respectivamente. O teor de MS influenciou a densidade de massa verde e MS, resultando em 346 e 105 kg.m-3 para silagens emurchecidas, 454 e 144 kg.m-3 naquelas adicionadas com milheto e 442 e 97 kg.m-3 com umidade original, respectivamente O uso do inoculante bacteriano diminuiu a RMS, não alterando o teor de PB e produção de amônia Silagens com adição do milheto tiveram suas frações fibrosas diluídas (FDN, FDA, CEL e HEMI). O experimento II avaliou o comportamento ingestivo e a digestibilidade dos nutrientes em bovinos Nelore providos de cânulas ruminal e duodenal recebendo rações com 87% de silagem de capim. Não houve efeito de tratamento sobre os valores médios de digestibilidade da MS (61,4%), FDA (59,7%), PB (43,9%) e FDN (60,3%), desaparecimento intestinal de MS (5,9%), FDN (3,0%) e FDA (4,4%), comportamento ingestivo, parâmetros de degradabilidade ruminal, parâmetros de cinética de passagem de sólidos e líquidos (2,4% e 3,2% hora-1), nem sobre o consumo médio (1,1% PV). O experimento III avaliou o efeito dos tratamentos sobre as propriedades físicas e perdas por deterioração da silagem de capim Tanzânia, sobre a ingestão de MS (IMS), o ganho de peso (GPV) e a conversão alimentar (CA) de novilhas Nelore alimentadas com rações completas contendo silagem de capim. A redução no tamanho da partícula não elevou as densidades de matéria seca (DMS) e de matéria verde (DMV) nem reduziu as perdas (P=0,60). O emurchecimento tendeu a diminuir a DMV (460 kg.m-3), porém não diminui a DMS, devido maior teor de MS, mas elevou as perdas em comparação ao tratamento com adição de milheto grão (29,1 vs 17,9%). Houve tendência (P=0,09) de aumento nas perdas na presença do inoculante bacteriano. Não houve efeito de tratamentos sobre a IMS (2,36% PV), GPV (1,03 kg.animal.dia-1) e CA (7,91 kg MS.kg GPV-1). Como os procedimentos adotados na ensilagem não tiveram efeito sobre a RMS, nem sobre os parâmetros ingestivos, digestivos e de desempenho dos animais, a opção pelas práticas a serem adotadas na ensilagem dependerá da ponderação de fatores que facilite a operacionalidade e reduza os custos na confecção da silagem. Sugere-se que a recomendação de práticas na ensilagem considere o controle de perdas integrado, desde a colheita da forragem até o desempenho de animais.
The present trial aimed to study the effect of dry matter content (DM), particle size and bacterial inoculant on the chemical-physical composition and conservation losses of Tanzania grass silage (Panicum maximum, Jacq. cv. Tanzânia), as well evaluated the ingestive behavior, nutrient digestion and performance of beef cattle. The experiment I analyzed the effects of treatments on silage fermentation and losses. DM recovery rate, effluent and gases losses were 90.58%; 53.66 kg.t wet forage-1 and 6.40% DM for wet silages, 93.62%; 16.81 kg.t wet forage-1 and 4.97% for millet added silages and 92.19%; 3.55 kg.t wet forage-1 and 6.16% for wilted silages, respectivelly. DM content influenced wet and dry silo bulk densities, resulting in 346 and 105 kg.m-3 for wilted silages, 454 and 144 kg.m-3 for millet aded silages and 442 and 97 kg.m-3 for wet silages, respectivelly. Bacterial inoculant led to a lower DM recovery and did not alter either CP content or the ammonia yield in the silages. Pearl millet addition determined lower fiber content in the silages (NDF, ADF, CEL and HEMI) due dilution effect. The experiment II acessed the ingestive behavior and nutrient digestion of Nellore beef steers ruminally and duodenally cannulated fed 87% silage TMR diets. The DM (61.4%), ADF (59.7%), CP (43.9%) and NDF (60.3%) total digestibilities, and the intestinal digestibilities for DM (5.9%), NDF (3.0%) and ADF (4.4%), ingestive behavior, in situ degradability, ruminal passage rate of solid and liquid phases (2,4% and 3,2% hour -1), and the mean DM intake (1,1% BW) were not significantly changed by treatments. Experiment III evaluated the effect of treatments on physical parameters and unloading losses of Tanzania grass silage, DM intake (DMI), performance (BWG) and feed conversion (FC) of Nellore heifers fed TMR containig grass silagen. By reducing the particle size no changes were observed neither in DM (DMD) and wet silo bulk (WD) nor on the unloading losses of silages (P=0.60). The forage wilting tended to reduce WD (460 kg.m-3), but did not changed the DMD, due to the compensatory higher DM content. However, wilting increased losses when compared to the addition of pearl millet (29.1 vs 17.9%). The addition of bacterial inoculant showed a trend (P=0.09) for increased losses. No significantlly differences were observed for the treatments in the DMI (2.4% BW), BWG (1.03 kg.animal.d-1) and FC (7.91 kg DM.kg BWG-1). The ensiling strategies established in Tanzânia grass silages did not result in significant changes neither in DMR nor in ingestive, digestive and animal performance parameters. According to the observed trend it might be concluded that the strategies adopted during the ensiling of Tanzania grass will be dependent on operational and costs restrictions. Some of these data are in conflict with the updated literature and because of this it might be suggested a decision based on the overall control of losses from the forage harvesting stage through the animal performance.

O conhecimento dos aspectos relacionados à interface planta:animal em sistemas de produção em pastagens ganha enorme relevância quando se tem como objetivo principal trabalhar dentro de princípios baseados no equilíbrio e racionalidade do uso dos recursos inerentes ao processo produtivo. Dessa forma, caracterizar os componentes da estrutura do dossel forrageiro e o efeito que os mesmos exercem sobre o comportamento ingestivo de animais em pastejo assume grande importância, uma vez que podem influenciar de forma relevante o consumo de forragem. Dentro desse contexto, o presente trabalho teve como objetivo procurar elucidar aspectos pertinentes à interface planta:animal através da quantificação do tamanho e da taxa de bocado, tempo gasto nas atividades de pastejo, ruminação e ócio pelos animais, e o consumo diário de forragem em pastos de Brachiaria brizantha cv. Marandu pastejados por bovinos em regime de lotação contínua e taxa de lotação variável. O experimento foi realizado em área do Departamento de Zootecnia da USP/ESALQ, em Piracicaba, SP, entre 01 de novembro de 2001 e 14 de fevereiro de 2003. Os tratamentos corresponderam a quatro alturas de dossel forrageiro (10, 20, 30 e 40 cm) mantidas em steady state, e foram alocados às unidades experimentais segundo um delineamento de blocos completos c casualizados, com quatro repetições. Os resultados revelaram uma redução em consumo de forragem com a diminuição da altura do dossel forrageiro (1,3; 1,8, 1,8 e 2,0 kg MS.kg PV -1 para 10, 20, 30 e 40 cm, respectivamente), conseqüência da redução em tamanho de bocado (0,5; 0,8; 1,2 e 1,5 g MS.bocado -1 para 10, 20, 30 e 40 cm, respectivamente). Os animais, na tentativa de tentar compensar a redução em consumo, aumentaram a taxa de bocado (46,3; 30,3; 23,8 e 17,5 bocados.minuto -1 para 10, 20, 30 e 40 cm, respectivamente) e, na condição de 10 cm de dossel forrageiro, aumentaram, também, o tempo de pastejo (11,4; 10,7; 10,6 e 10,5 horas.dia -1 para 10, 20, 30 e 40 cm, respectivamente). Concluiu-se que a ingestão de forragem pode ser controlada através de variações em condição e estrutura do dossel forrageiro, situação essa que demonstra o potencial de planejamento e monitoramento de estratégias de pastejo baseadas em metas de condição de dossel para níveis variáveis de exigências nutricionais, épocas do ano e espécie animal.
Knowledge of aspects related to plant:animal interface in pastoral systems acquire greater importance when the main objective is to rationalize and optimize the use of any resource available. In that scenario, characterization of structural sward components and their effect on ingestive behaviour of grazing animals is a key element, since they can have a relevant influence on herbage intake. Against this background, the objective of this experiment was to study aspects of the plant:animal interface quantifying bite size, bite rate, time spent on grazing, rumination and rest as well as herbage intake of cattle grazing Brachiaria brizantha cv Marandu pastures submitted to continuous stocking managements. The experiment was carried out at Departamento de Zootecnia, USP/ESALQ, Piracicaba, SP, from 01 November 2001 until 14 February 2003. Treatments corresponded to four steady state conditions characterized by sward surface heights (SSH) of 10, 20, 30 and 40 cm, assigned to experimental units according to a complete randomized block design, with four replications. The results revealed a reduction in herbage intake with decreasing SSH (1.3, 1.8, 1.8 and 2.0 kg DM.kg LW -1 for 10, 20, 30 and 40 cm, respectively), consequence of a reduction in bite size (0.5, 0.8, 1,2 and 1.5 g DM.bite -1 for 10, 20, 30 and 40 cm, respectively). Animals, in an attempt to compensate the decrease in herbage intake, increased their bite rate (46.3, 30.3, 23.8 and 17.5 bites.minute -1 for 10, 20, 30 and 40 cm, respectively) and, for the 10 cm SSH, increased, also, their grazing time (11.4, 10.7, 10.6 and 10.5 hours.day -1 for 10, 20, 30 and 40 cm, respectively). It was concluded that herbage intake can be controlled by variations in sward structure and condition, which points to the potential for planning and monitoring grazing strategies based on sward targets for variable levels of animal requirements, time of the year and animal species.

Num ecossistema de pastagem a interceptação da luz incidente é o passo inicial de uma seqüência de eventos que resulta na produção de forragem. Vários são os fatores que interferem no processo de interceptação da luz e, dentre eles, os mais expressivos são aqueles relacionados com o índice de área foliar, com as características estruturais e com o arranjo espacial de componentes do dossel forrageiro. O presente experimento teve como objetivo estudar a estrutura do dossel, a interceptação luminosa e o acúmulo de forragem em pastos de Brachiaria brizantha cv. Marandu submetidos a lotação contínua por bovinos de corte. O experimento foi conduzido no Departamento de Zootecnia da USP/ESALQ. Os tratamentos corresponderam a quatro alturas de dossel (10, 20, 30 e 40 cm) mantidas constantes por meio de lotação contínua e taxa de lotação variável. O delineamento experimental foi o de blocos completos casualizados com quatro repetições. As variáveis estudadas foram: índice de área foliar (IAF), ângulo foliar, interceptação de luz, composição botânica/morfológica da forragem, distribuição vertical dos componentes do dossel forrageiro e acúmulo de forragem. A composição da massa de forragem dos pastos não apresentou grande variação entre tratamentos, principalmente no que diz respeito à porcentagem de hastes (27%) e de folhas (21%). A quantidade absoluta e a porcentagem de material morto em relação aos outros componentes morfológicos do dossel aumentaram do verão (30%) até o início da primavera (61%). Quanto maior a altura do dossel, maior foi o deslocamento dos componentes morfológicos ao longo de seu perfil vertical durante o ano. Reduções acentuadas nos valores de IAF dos pastos mantidos a 20 (5,2 para 1,6), 30 (7,0 para 1,5) e 40 cm (7,5 para 2,1) do verão ao início da primavera, respectivamente, não foram acompanhadas por reduções em interceptação luminosa, que se manteve consistentemente acima de 98%. O componente material morto interferiu de foma significativa na interceptação de luz, além de ter apresentado um padrão “dinâmico” de posicionamento no perfil vertical do dossel ao longo do ano. Essa variação na estrutura vertical do dossel foi tão maior quanto mais altos foram mantidos os pastos, e seu efeito sobre os padrões de interceptação luminosa provocou grandes variações na produção de forragem ao longo do ano. De toda a forragem produzida, 65 e praticamente 100% foi acumulada durante o final da primavera e verão nos pastos mantidos a 10 e 40 cm, respectivamente. A produção total de forragem não diferiu entre os pastos mantidos a 10, 20 e 30 cm (aproximadamente 24 ton.ha-1.ano-1), sugerindo haver grande flexibilidade de manejo para essa espécie forrageira. As modificações no sentido vertical e horizontal na estrutura do dossel forrageiro tiveram impacto maior sobre a distribuição estacional do que propriamente sobre a produção total de forragem.
In a pastoral ecossystem the interception of incident radiation is the first step of a sequence of events that results in herbage production. Several are the factors interfering on the light interception process including sward leaf area index, structure and architecture. The objective of this experiment was to study sward struture, light interception and herbage acummulation of Brachiaria brizantha cv. Marandu pastures submitted to continuous stocking by beef cattle. The experiment was carried out at Departamento de Zootecnia, USP/ESALQ. Treatments correspondended to four sward heights (10, 20, 30 and 40 cm) kept constant through continuous stocking and variable stocking rate, allocated to experimental units according to a complete randomized block design with four replications. The response variables evaluated were sward leaf area index (LAI), foliage angle, light interception, botanical and morphological composition and hebarge production. There was no significant change in hebarge mass composition with stems representing 27% and leaves 21% of the sward. Quantity and proportion of dead material relative to the other sward morphological components increased from Summer (30%) to early Spring (61%). The taller the swards, the larger was the shift in the position of the morphological components across the vertical sward profile throughout the year. Significant reductions in LAI values for the 20 cm (5,2 to 1,6), 30 cm (7,0 to 1,5) and 40 cm swards (7,5 to 2,1) from Summer to early Spring were not followed by reduction in light interception, which remained above 98% consistent by through out the year around. The dead material component played an important role on sward light interception, and its placement across the vertical sward profile exhibited a “dynamic” pattern of variation. This variation was more evident in taller swards and affected sward light interception, resulting in varying seasonalities of herbage production. From the total herbage yield, 65 and 100% was produced during late Spring and Summer on the 10 and 40 cm swards, respectively. There was no difference in total herbage production among swards maintained at 10, 20 and 30 cm (24 ton.ha-1.year-1), sugesting that there is a large flexibilitty in grazing management for Brachiaria brizantha cv. Marandu. Modifications in both vertical and horizontal structure of the sward were more effective in changing the seasonal pattern of herbage production than determining herbage yield.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
O objetivo deste trabalho foi avaliar os DDGS (do inglês “distillers dried grains with solubles”) como fonte de proteína não degradável no rúmen (PNDR) em substituição ao farelo de algodão em suplementos para bovinos em pastagens de Brachiaria brizantha cv. Marandu na fase de recria durante o período das águas. Foram utilizados 8 piquetes de Brachiaria brizantha cv. Marandu, manejados em lotação contínua, com taxa de lotação variável. Oito bovinos Bos indicus fistulados no rúmen foram utilizados para avaliar quatro estratégias de suplementação: Tratamento 1) mistura mineral (ad libitum) (MM); Tratamento 2) suplemento proteico e energético com milho como fonte energética e farelo de algodão como fonte proteica (FA); Tratamento 3) suplemento com substituição de 50% da fonte proteica do Tratamento 2 por DDGS (50DDG) e Tratamento 4) suplemento com substituição de 100% da fonte proteica do Tratamento 2 por DDGS (100DDG). Com exceção do MM (MM), os suplementos foram fornecidos na quantidade de 0,3% do peso corporal (PC). O delineamento estatístico adotado foi quadrado latino duplo, com oito animais, quatro tratamentos, e quatro períodos experimentais. Foi aplicado o teste de contrastes (MM vs (FA+50DDG+100DDG); efeito linear entre FA, 50DDG e 100DDG; efeito quadrático entre FA, 50DDG e 100DDG) (P<0,10). Houve diferença no consumo de matéria seca (MS) (P= 0,0130), de nutrientes (P≤0,099) e do balanço de nitrogênio (P=0,019) entre MM e os demais tratamentos. Observou-se efeito linear decrescente (P=0,081) entre FA, 50DDG e 100DDG nos teores de nitrogênio amoniacal ruminal. Nos demais parâmetros avaliados não houve diferença significativa (P≥0,10). A suplementação proteica energética aumentou o consumo total de MS e de nutrientes, porém sem alterar a digestibilidade. Os tratamentos avaliados não influenciaram os parâmetros ruminais, assim como a síntese de proteína microbiana, mas a suplementação proteico energética com 0,3% PC aumentou a eficiência de uso do nitrogênio.
The aim of this study was to evaluate the intake, apparent total diet digestibility, ruminal parameters, nitrogen balance and microbial protein synthesis of beef cattle supplemented with different protein sources (cottonseed meal or DDG “distillers dried grains with solubles") on pasture during the wet season. Eight paddock of Brachiaria brizantha cv. Marandu (Palisade grass), were managed in a continuous grazing, with a variable stocking rate. Eight rumen cannulated steers were used for evaluations of the four supplementation strategies: Treatment 1) mineral mixed (ad libitum) (MM); Treatment 2) energetic/protein supplement with corn as energetic source and cottonseed meal as protein source (FA); Treatment 3) supplement with substitution of 50%of the protein source of treatment 2forDDGS (50DDG), and Treatment 4) supplement with replacement of 100% of the protein source of treatment 2 for DDGS (100DDG). Except for MM, supplements were provided in amount of 0.3% of body weight (BW). The statistical design adopted was a double Latin square, with eight cannulated animals, four treatments, and four experimental periods. The test of contrasts (MM vs. FA + 50DDG + 100DDG), linear effect among FA, 50DDG and 100DDG) and quadratic effect among FA, 50DDG and 100DDG) was performed (P <0, 10). There was a significant difference in dry matter (DM) and nutrient intake, and nitrogen balance among MM and other treatments. There was a linear decrease effect among FA, 50DDG and 100DDG in ruminal ammonia nitrogen levels. In the other parameters evaluated there was no significant difference. It is concluded that the evaluated treatments did not affected the ruminal parameters, as well as the synthesis of microbial protein, but the supplementation with 0.3% CP increased the efficiency of nitrogen utilization.
CNPq 132885/2016-6

O estudo teve como objetivo avaliar os efeitos de diferentes níveis de suplementação energética sobre os parâmetros metabólicos (ruminais e sanguíneos), consumo de forragem, comportamento animal e cinética de fermentação ruminal de novilhos Nelore recriados em pastagem tropical na época das águas. Os tratamentos foram: T1) controle (somente suplementação mineral); T2) 0,3% PC; T3) 0,6% PC; e T4) 0,9% PC em milho moído fino e monensina sódica. Foram utilizados 8 novilhos Nelore, fistulados no rúmen, com aproximadamente 410 kg e 48 meses de idade. O delineamento estatístico utilizado foi o de quadrado latino, com 2 quadrados latinos 4x4. Os animais foram alocados em 1 ha de pastagem de capim Brachiaria brizantha cv. Marandu. Os parâmetros avaliados foram o consumo de forragem, comportamento animal, pH e amônia ruminais, concentrações de ácidos graxos de cadeia curta (AGCC), teores plasmáticos de glicose e uréia, cinética de degradação ruminal, síntese de proteína microbiana e excreção de nitrogênio. Dois marcadores foram testados para estimar o consumo, LIPE e óxido crômico, e o escolhido para estimar o consumo foi o óxido crômico. A suplementação energética reduziu linearmente (P<0,05) o consumo de forragem, mas aumentou o consumo total de MS e de NDT (P<0,05). As taxas de substituição foram mais altas no nível 0,3%, intermediária no nível 0,6% e mais baixa no nível 0,9% do PC (P<0,05). Entre as variáveis do comportamento animal houve efeito apenas para o tempo de pastejo (P<0,05), que foi reduzido, e para o tempo de ócio (P<0,10) que foi aumentado com a suplementação. Houve efeito da suplementação apenas na produção de propionato, entre os AGCC, sendo aumentada linearmente (P<0,05) em função da suplementação. O pH não foi alterado em função dos níveis de suplementação, estando entre valores recomendados para manutenção dos microrganismos celulolíticos. A concentração ruminal de N-NH3 e a excreção de nitrogênio foram reduzidas com a suplementação (P<0,05). A síntese de proteína microbiana foi aumentada (P<0,05) em função dos níveis crescentes de suplementação, o que demonstra o efeito da sincronia de energia e proteína no rúmen. Não houve efeito significativo para os parâmetros sanguíneos (glicose e uréia). A suplementação melhorou a degradação da fração fibrosa, aumentando a fração potencialmente degradável da FDN (P<0,05), e reduzindo a fração indigestível da FDN. Não houve efeito (P<0,05) no lag time para a degradação da fibra. A redução no consumo de forragem em função da suplementação energética não está associada a prejuízos no pH ruminal e na degradação da fração fibrosa. A suplementação energética de bovinos de corte mantidos em pastagens de bom valor nutritivo e manejadas intensivamente no período das águas promove aumentos na síntese de proteína microbiana e melhorias na degradação da fibra.
The objective of this trial was to evaluate the effects of different levels of energy supplementation on metabolic parameters (blood and ruminal), forage intake, animal behavior and ruminal kinetics of Nelore steers raised in tropical pasture during the rainy season. Each level of supplementation was considered a treatment and consisted of: T1) control (only mineral supplementation); T2) supply of 0.3% BW of energetic concentrate; T3) supply of 0.6% of BW of energetic concentrate; T4) supply of 0.9% of BW of energetic concentrate. Eight Nelore rumen-cannulated steers, averaging 410 kg BW and 48 months of age were assigned to two 4x4 Latin square and allotted in 1 ha of Brachiaria brizantha cv. Marandu pasture. The parameters evaluated were forage intake, ruminal pH and concentration of NH3 and short chain fatty acids (SCFA), blood nitrogen urea and blood glucose, ruminal kinetics, microbial synthesis, nitrogen excretion and animal behavior. The marker that better estimated the intake was chromium oxide. Forage intake (% BW) decreased (P<0.05) and total DM intake increased (P<0.05) as levels of supplementation increased. Substitution rates decreased (P<0.05) as the supplementation level increased, explaining the higher total DM intake. Among the ingestive behavior variables, only grazing time, that decreased (P<0.05), and rest time, that increased (P<0.10), as the supplement level increased, were affected by supplementation. Among the SCFA, only the propionate concentration was affected (P<0.05) by the treatments, increasing as the levels increased. The ruminal pH was not affected by supplementation and was among the recommended values for maintenance of cellulolytic microorganisms. The concentration of NH3 and nitrogen excretion decreased (P<0.05), and the microbial synthesis increased (P<0.05) as levels of supplement increased, as a result of the better synchronization between energy and protein in the rumen. Blood variables (glucose and urea nitrogen content) were not affected by treatments. Supplementation improved fiber degradation, by increasing the potential degradable fraction of NDF (P<0.05). There was no effect on the lag time of fiber degradation, confirming that the supplementation had no negative effects on the ruminal environment. The decreasing on forage intake as the supplement level increased was not associated neither to inadequate ruminal pH nor to impairment of fiber degradation. Energetic supplementation for beef cattle grazing tropical pastures intensively managed during rainy season improves microbial synthesis and fiber degradation.

In KwaZulu-Natal the production of beef in summer from veld is a common enterprise. Many techniques are available to optimise the productivity of this enterprise, from improving the quality of the grazing resource (planted pastures) to improving the diet of animals using nutritional supplements. To gain an insight into the production potential and financial returns possible from such improvements a trial was established at the Ukulinga Research Farm during two consecutive summer growing seasons (1997-1998 and 1998-1999). The aim was to determine the optimum beef production system for the area from both veld and kikuyu pastures. To evaluate the benefits of supplying supplementary nutrition, four alternate feed supplements, namely: 1) a Standard commercially available molasses-based protein/mineral/energy supplement; 2) a Brewers grain based protein/mineral/energy supplement, and two supplements consisting of the Brewers grain ration with either; 3) Avoparcin (an additive that improves dietary energy) or 4) Bentonite (an additive that increases the bypass of protein) were compared. As stocking rate has been shown to influence the quality of the diet consumed, the kikuyu pasture was grazed at both the recommended (1.92 LSD ha¯¹) and half the recommended stocking rate for the region. In addition, a commercial hormonal implant was applied to half of the cattle in each treatment. Grazing was monitored using the falling plate disc meter to measure pasture bulk density and laboratory analyses of herbage grab samples for digestibility and crude protein percentage. Cattle were weighed on a weekly basis and their condition was scored prior to slaughter. All enterprise costs and returns were recorded to facilitate financial analyses of the five treatments. Low rainfall and high midsummer temperatures had a detrimental effect on the productivity of the grazing and hence it was difficult to optimise production in either season. In the first season, a midsummer drought decreased the quality and quantity of both veld and kikuyu, limiting mass gain during the latter part of the season. A delay in the onset of rain at the start of the second season limited the available grazing season to 121 days as opposed to 154 days, though fodder production during the season was not limited. Trends in herbage production (quality and quantity) from veld showed moderate quality (Crude protein 7.02%; digestibility 50.2%) with an average available herbage of 1670 kg DM ha¯¹. As anticipated, kikuyu had higher quality (Crude protein 10.84%; digestibility 53.5%) and available herbage (2730 kg DM ha¯¹). These results were similar to regional benchmarks. The variable rainfall highlighted both the drought tolerance of veld and the minimum water requirements of kikuyu pastures. Lighter stocking rates tended to reduce the negative impact of moisture stress on Kikuyu pastures. The best method of producing beef (averaged over two seasons) was from heavily stocked Kikuyu pastures using the Standard supplement (1107.63 kg livemass ha¯¹). Cattle grazing veld and utilising the Avoparcin supplement produced beef at a rate of 95.96 kg ha¯¹. In comparison, the unsupplemented cattle grazing Kikuyu produced 834.87 kg ha¯¹, whilst veld grazing produced 64.43 kg ha¯¹. Hormonal implants significantly (P≤0.05) improved beef production from all sources of grazing. A lack of rain limited grazing time, causing all the cattle to be marketed whilst too lean - this negatively affected live mass gain and, hence, net financial. Although improved biological production is desirable, it is important to ensure that these gains are financially sustainable. Within the trial environment, implanted cattle fed the Standard supplement and grazing Kikuyu pastures at a high stocking rate provided the highest average gross margin of R 859.59 ha¯¹. Changing to this production system from unsupplemented veld improved expected profit by R 632.58 ha¯¹ (averaged over both seasons). Further financial analyses indicated that beef purchase price had the greatest influence on the added profit from switching from the control treatment. From a scientific standpoint these data are conclusive but it is important to remember that consumer pressure and concerns can often limit the introduction of production improvements. Such is the case with both hormonal implants and antibiotic feed additives (Avoparcin) although considering the impact of such limitations is speculative and beyond the scope of this trial.
Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2004.

Kikuyu pasture (Pennisetum clandestinum) is potentially the most important source of roughage used to feed dairy heifers in summer in KwaZulu-Natal. It is commonly believed that on kikuyu pasture beef breed females grow at a faster rate than those from dairy breeds when no supplementation is given. Little conclusive evidence is, however, available to support this. Explanations as to why such differences may exist are also limited. Eight Hereford and eight Holstein heifers of similar age and maturity stage were used in a trial. The trial was run over a twenty week period. For the first ten weeks all the animals in the trial grazed ad libitum kikuyu pasture with no supplementation except for a mineral lick. Over this (grass only) period the two breed groups formed the two treatments. During the second ten week period of the trial all of the Holsteins and four of the Herefords were fed a restricted but equivalent amount (1 .7 kg) of a maize meal based concentrate. The use of a computerized, mobile feeding system allowed concentrate intake of individual animals to be measured. Animal height, weight and condition score readings were taken weekly over the grass only and the concentrate (final seven weeks) periods of the trial. Herbage intake and digestibility were estimated using n-alkanes as indigestible markers in two experiments conducted during the grass only and concentrate periods. The Herefords had a significantly higher ADG than the Holsteins (0.82 vs. 0.04 kg/day; P < 0.01) over the grass only period. During the concentrate period the rate of mass gain of the Holstein treatment did not differ significantly (P >0.05) from the Hereford treatment receiving concentrate. The Herefords receiving concentrate were also not significantly different (P > 0.05) in rate of mass gain from the Herefords not receiving concentrate. Rate of height gain was not significantly different (P> 0.05) between treatments over either the concentrate or the grass only periods. During the grass only period the Holsteins lost condition (0.07 condition score units per week) whilst the Herefords gained condition at an equivalent rate. The voluntary intake of concentrates was not significantly different (P > 0.05) between the Herefords and Holsteins (19.19 vs. 16.40 g/kg/L.W(liveweight) (0.75)). Regression coefficients relating level of concentrate intake to rate of mass gain were also not significant (P > 0.05) for either of the treatments receiving concentrate. The use of n-alkanes as indigestible markers showed the intake of the Holstein treatment to have an intake 55% (P < 0.0 1) higher than the Herefords (185.4 vs. 120.5 g/kg L.W(0.75)) over the first experiment where both treatments were grazing ad lib. kikuyu alone (grass only period). During the concentrate period intake of the Herefords receiving concentrate exceeded that of the Holsteins (P < 0.01) by 23% (139.1 vs. 113.1 g/kg L.W(0.75)). Review of the literature, suggests that the double alkanes technique greatly over-estimated intake. Errors in herbage sampling (accentuated by pasture rotation in the first experiment), a low daily dose of the synthetic alkane (C(32)) and incorrect estimation of the C(32) content in the daily doses are identified as possible causes of the over-estimation of intake. Faecal recoveries of the herbage n-alkanes were demonstrated to increase with increasing chain length and hence C(35) was proposed as the most reliable herbage alkane for dry matter digestibility determination. Digestibility differences between treatments estimated using the C(35) alkane were not significantly different (P > 0.05) in either the first or second experiments. The mean digestibility estimates (using the C(35) alkane) for the first and second experiments were 64.9 and 56.61 %, respectively. In conclusion, higher growth rates of Herefords on kikuyu pasture would seem to be primarily due to differences in the dry matter intake of the grazed herbage. Further work using other breeds of dairy and beef animals is required. The underlying cause of differences in dry matter intake between breeds also requires investigation.
Thesis (M.Sc.Agric.)-University of Natal, Pietermaritzburg, 1995.

South African dairy farmers have generally used kikuyu pasture to tide them over from one ryegrass season to the next, and as a result of its resilient nature, have assumed careful management of it to be unnecessary. This has resulted in its mismanagement which is unaffordable in current times where the profitability of dairy farming is increasingly dependent on low input, pasture-based systems. Kikuyu pasture may play a larger role in supplying nutrients to dairy cattle over the summer months in future as the alternative home produced feed sources such as silage and perennial ryegrass become increasingly unaffordable. Improving animal production from kikuyu is difficult as there is little information relating kikuyu pasture management to dairy cow performance. Efficient utilization and quality of temperate pasture have been more comprehensibly researched. The relations discovered between the chemical compounds in temperate grass species have been applied to tropical pastures such as kikuyu with limited success and often confusing results. For example, crude fibre in kikuyu was found to be positively related to digestibility. In South Africa, much research has been done on the use of kikuyu in beef production systems. This information has been applied to dairy farming systems with limited success, owing to the higher metabolic demands of dairy animals. Pasture farming needs to become more precise to improve pasture quality and hence milk yields as research trials focussing on stocking rate and grazing system comparisons have yielded results that are too general with little application at the farming level. A need for integrated and flexible management of animals and pastures has been recognised. The grazing interval is a key aspect in improving pasture and animal performance and fixed rotation lengths and stocking rates have been identified as being detrimental to performance. The relation between growth stage and pasture quality has lead researchers to identify plant growth characteristics, such as pasture height and leaf stage, as signs of grazing readiness. At the four and a half leaves per tiller stage of regrowth, the chemical composition ofthe kikuyu plant is more in line with the requirements ofthe dairy cow, with the leaf to stem ratio at its highest. The primary limitation of kikuyu pasture is a lack of energy, particularly readily fermentable carbohydrate, which makes the fermentation of structural carbohydrates difficult and dry matter intakes are reduced. Other limitations to animal performance include high cell wall constituents, low calcium, magnesium and sodium content and antinutritional factors such as nitrate and insoluble oxalate. These deficiencies and antinutritional factors are in some cases unique to 5 kikuyu pasture, meaning that kikuyu specific supplementation may be the key to improving performance from dairy cattle grazing kikuyu pasture. The objectives are to evaluate current kikuyu management systems in South Africa and their impact on dairy cow performance and to evaluate the use of pasture height and burning as quality control tools.
Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2007.