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Artículos de revistas sobre el tema "Biofilm cultures"

Autor: Grafiati

Publicado: 4 de junio de 2021

Última modificación: 10 de febrero de 2022

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1

SHEFFIELD, C. L., T. L. CRIPPEN, K. ANDREWS, R. J. BONGAERTS, and D. J. NISBET. "Planktonic and Biofilm Communities from 7-Day-Old Chicken Cecal Microflora Cultures: Characterization and Resistance to Salmonella Colonization†." Journal of Food Protection 72, no. 9 (September 1, 2009): 1812–20. http://dx.doi.org/10.4315/0362-028x-72.9.1812.

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Information implicating bacterial biofilms as contributory factors in the development of environmental bacterial resistance has been increasing. There is a lack of information regarding the role of biofilms within the microbial ecology of the gastrointestinal tract of food animals. This work used a continuous-flow chemostat model derived from the ceca of 7-day-old chicks to characterize these communities and their ability to neutralize invasion by Salmonella enterica serovar Typhimurium. We characterized and compared the biofilm and planktonic communities within these microcosms using automate

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Schooling, S. R., U. K. Charaf, D. G. Allison, and P. Gilbert. "A role for rhamnolipid in biofilm dispersion." Biofilms 1, no. 2 (April 2004): 91–99. http://dx.doi.org/10.1017/s147905050400119x.

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Biofilms are often considered as localized zones of high cell density. Quorum sensing provides a means for control of population processes and has been implicated in the regulation of biofilm activities. We present a role for quorum sensing in programmed detachment and dispersal processes. Biofilms of Pseudomonas aeruginosa PAO1 and its isogenic homoserine lactone (HSL) mutant P. aeruginosa PAO-JP2 were grown in batch culture on glass substrata; differences were found in the rate and extent of formation of biofilm. Climax communities were observed for PAO1 at 24 h. These were later accompanied

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Frederick, Jesse R., James G. Elkins, Nikki Bollinger, Daniel J. Hassett, and Timothy R. McDermott. "Factors Affecting Catalase Expression in Pseudomonas aeruginosa Biofilms and Planktonic Cells." Applied and Environmental Microbiology 67, no. 3 (March 1, 2001): 1375–79. http://dx.doi.org/10.1128/aem.67.3.1375-1379.2001.

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ABSTRACT Previous work with Pseudomonas aeruginosa showed that catalase activity in biofilms was significantly reduced relative to that in planktonic cells. To better understand biofilm physiology, we examined possible explanations for the differential expression of catalase in cells cultured in these two different conditions. For maximal catalase activity, biofilm cells required significantly more iron (25 μM as FeCl3) in the medium, whereas planktonic cultures required no addition of iron. However, iron-stimulated catalase activity in biofilms was still only about one-third that in planktoni

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MINEI, CLÁUDIA C., BRUNA C. GOMES, REGIANNE P. RATTI, CARLOS E. M. D'ANGELIS, and ELAINE C. P. DE MARTINIS. "Influence of Peroxyacetic Acid and Nisin and Coculture with Enterococcus faecium on Listeria monocytogenes Biofilm Formation." Journal of Food Protection 71, no. 3 (March 1, 2008): 634–38. http://dx.doi.org/10.4315/0362-028x-71.3.634.

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Biofilm formation is a matter of concern in food industries because biofilms facilitate the survival of pathogenic bacteria such as Listeria monocytogenes, which may contaminate food-processing equipment and products. In this study, nisin and two Enterococcus faecium strains were evaluated for their effect on biofilm formation by L. monocytogenes cultured in brain heart infusion broth and on stainless steel coupons. Elimination of preformed L. monocytogenes biofilms by peroxyacetic acid also was tested. Adhesion control experiments were performed with pure cultures of L. monocytogenes after sw

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Vidal, Jorge E., Joshua R. Shak, and Adrian Canizalez-Roman. "The CpAL Quorum Sensing System Regulates Production of Hemolysins CPA and PFO To Build Clostridium perfringens Biofilms." Infection and Immunity 83, no. 6 (March 30, 2015): 2430–42. http://dx.doi.org/10.1128/iai.00240-15.

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Clostridium perfringensstrains produce severe diseases, including myonecrosis and enteritis necroticans, in humans and animals. Diseases are mediated by the production of potent toxins that often damage the site of infection, e.g., skin epithelium during myonecrosis. In planktonic cultures, the regulation of important toxins, such as CPA, CPB, and PFO, is controlled by theC. perfringensAgr-like (CpAL) quorum sensing (QS) system. Strains also encode a functional LuxS/AI-2 system. AlthoughC. perfringensstrains form biofilm-like structures, the regulation of biofilm formation is poorly understood

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Wolyniak, E. A., B. R. Hargreaves, and K. L. Jellison. "Retention and Release of Cryptosporidium parvum Oocysts by Experimental Biofilms Composed of a Natural Stream Microbial Community." Applied and Environmental Microbiology 75, no. 13 (May 15, 2009): 4624–26. http://dx.doi.org/10.1128/aem.02916-08.

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ABSTRACT Cryptosporidium parvum oocysts accumulate on biofilm surfaces. The percentage of oocysts attached to biofilms remained nearly constant while oocysts were supplied to the system but decreased to a new steady-state level once oocysts were removed from the feed. More oocysts attached to summer biofilm cultures than winter biofilm cultures.

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Rahmani-Badi, Azadeh, Shayesteh Sepehr, Parisa Mohammadi, Mohammad Reza Soudi, Hamta Babaie-Naiej, and Hossein Fallahi. "A combination of cis-2-decenoic acid and antibiotics eradicates pre-established catheter-associated biofilms." Journal of Medical Microbiology 63, no. 11 (November 1, 2014): 1509–16. http://dx.doi.org/10.1099/jmm.0.075374-0.

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The catheterized urinary tract provides ideal conditions for the development of biofilm populations. Catheter-associated urinary tract infections (CAUTIs) are recalcitrant to existing antimicrobial treatments; therefore, established biofilms are not eradicated completely after treatment and surviving biofilm cells will carry on the infection. Cis-2-decenoic acid (CDA), an unsaturated fatty acid, is capable of inhibiting biofilm formation by Pseudomonas aeruginosa and of inducing the dispersion of established biofilms by multiple types of micro-organisms. Here, the ability of CDA to induce disp

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Bryers, James D., and Huang Ching-Tsan. "Recombinant plasmid retention and expression in bacterial biofilm cultures." Water Science and Technology 31, no. 1 (January 1, 1995): 105–15. http://dx.doi.org/10.2166/wst.1995.0025.

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Any exposure of plasmid recombinant microorganisms to an open system environment, either inadvertently or intentionally, mandates research into those fundamental organism:plasmid processes that influence plasmid retention, transfer and expression. In open environmental systems a majority of the microbial activity occurs associated with an interface, within thin biological layers consisting of the cells and their insoluble extracellular polymer, layers known as biofilms. Thus any study regarding the fate of recombinant DNA sequences in an open system must consider processes that affect plasmid

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Chinnici, Jennifer, Lisa Yerke, Charlene Tsou, Sujay Busarajan, Ryan Mancuso, Nishanth D. Sadhak, Jaewon Kim, and Abhiram Maddi. "Candida albicans cell wall integrity transcription factors regulate polymicrobial biofilm formation with Streptococcus gordonii." PeerJ 7 (October 11, 2019): e7870. http://dx.doi.org/10.7717/peerj.7870.

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Polymicrobial biofilms play important roles in oral and systemic infections. The oral plaque bacterium Streptococcus gordonii is known to attach to the hyphal cell wall of the fungus Candida albicans to form corn-cob like structures in biofilms. However, the role of C. albicans in formation of polymicrobial biofilms is not completely understood. The objective of this study was to determine the role of C. albicans transcription factors in regulation of polymicrobial biofilms and antibiotic tolerance of S. gordonii. The proteins secreted by C. albicans and S. gordonii in mixed planktonic culture

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Kay, Matthew K., Thomas C. Erwin, Robert J. C. McLean, and Gary M. Aron. "Bacteriophage Ecology inEscherichia coliandPseudomonas aeruginosaMixed-Biofilm Communities." Applied and Environmental Microbiology 77, no. 3 (December 3, 2010): 821–29. http://dx.doi.org/10.1128/aem.01797-10.

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ABSTRACTPhage therapy is being reexamined as a strategy for bacterial control in medical and other environments. As microorganisms often live in mixed populations, we examined the effect ofEscherichia colibacteriophage λW60 andPseudomonas aeruginosabacteriophage PB-1 infection on the viability of monoculture and mixed-species biofilm and planktonic cultures. In mixed-species biofilm communities,E. coliandP. aeruginosamaintained stable cell populations in the presence of one or both phages. In contrast,E. coliplanktonic populations were severely depleted in coculture in the presence of λW60. Bo

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Chao, Jerry, Gideon M. Wolfaardt, and Michael T. Arts. "Characterization of Pseudomonas aeruginosa fatty acid profiles in biofilms and batch planktonic cultures." Canadian Journal of Microbiology 56, no. 12 (December 2010): 1028–39. http://dx.doi.org/10.1139/w10-093.

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The fatty acid composition of Pseudomonas aeruginosa PAO1 was compared between biofilm and batch planktonic cultures. Strain PAO1 biofilms were able to maintain a consistent fatty acid profile for up to 6 days, whereas strain PAO1 batch planktonic cultures showed a gradual loss of cis-monounsaturated fatty acids over 4 days. Biofilms exhibited a greater proportion of hydroxy fatty acids but a lower proportion of both cyclopropane fatty acids and saturated fatty acids (SAFAs). SAFAs with ≥16 carbons, in particular, decreased in biofilms when compared with that in batch planktonic cultures. A re

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Vacheva, Anna, Ralitsa Georgieva, Svetla Danova, Radka Mihova, Mariana Marhova, Sonia Kostadinova, Krasimira Vasileva, Maria Bivolarska, and Stoyanka Stoitsova. "Modulation of Escherichia coli biofilm growth by cell-free spent cultures from lactobacilli." Open Life Sciences 7, no. 2 (April 1, 2012): 219–29. http://dx.doi.org/10.2478/s11535-012-0004-9.

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AbstractE. coli biofilms cause serious problems in medical practice by contaminating surfaces and indwelling catheters. Due to the rapid development of antibiotic resistance, alternative approaches to biofilm suppression are needed. This study addresses whether products released by antagonistic bacteria — Lactobacillus isolates from vaginal and dairy-product samples could be useful for controlling E. coli biofilms. The effects of diluted cell-free supernatants (CFS) from late-exponential Lactobacillus cultures on the growth and biofilm production of Escherichia coli were tested. Most of the CF

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Rosca, Aliona S., Joana Castro, and Nuno Cerca. "Evaluation of different culture media to support in vitro growth and biofilm formation of bacterial vaginosis-associated anaerobes." PeerJ 8 (September 10, 2020): e9917. http://dx.doi.org/10.7717/peerj.9917.

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Background Bacterial vaginosis (BV) is one of the most common vaginal infections worldwide. It is associated with the presence of a dense polymicrobial biofilm on the vaginal epithelium, formed mainly by Gardnerella species. The biofilm also contains other anaerobic species, but little is known about their role in BV development. Aim To evaluate the influence of different culture media on the planktonic and biofilm growth of six cultivable anaerobes frequently associated with BV, namely Gardnerella sp., Atopobium vaginae, Lactobacillus iners, Mobiluncus curtisii, Peptostreptococcus anaerobius

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Behnke, Sabrina, Albert E. Parker, Dawn Woodall, and Anne K. Camper. "Comparing the Chlorine Disinfection of Detached Biofilm Clusters with Those of Sessile Biofilms and Planktonic Cells in Single- and Dual-Species Cultures." Applied and Environmental Microbiology 77, no. 20 (August 19, 2011): 7176–84. http://dx.doi.org/10.1128/aem.05514-11.

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ABSTRACTAlthough the detachment of cells from biofilms is of fundamental importance to the dissemination of organisms in both public health and clinical settings, the disinfection efficacies of commonly used biocides on detached biofilm particles have not been investigated. Therefore, the question arises whether cells in detached aggregates can be killed with disinfectant concentrations sufficient to inactivate planktonic cells.Burkholderia cepaciaandPseudomonas aeruginosawere grown in standardized laboratory reactors as single species and in coculture. Cluster size distributions in chemostats

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Nuryastuti, Titik, Henny C. van der Mei, Henk J. Busscher, Susi Iravati, Abu T. Aman, and Bastiaan P. Krom. "Effect of Cinnamon Oil on icaA Expression and Biofilm Formation by Staphylococcus epidermidis." Applied and Environmental Microbiology 75, no. 21 (September 11, 2009): 6850–55. http://dx.doi.org/10.1128/aem.00875-09.

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ABSTRACT Staphylococcus epidermidis is notorious for its biofilm formation on medical devices, and novel approaches to prevent and kill S. epidermidis biofilms are desired. In this study, the effect of cinnamon oil on planktonic and biofilm cultures of clinical S. epidermidis isolates was evaluated. Initially, susceptibility to cinnamon oil in planktonic cultures was compared to the commonly used antimicrobial agents chlorhexidine, triclosan, and gentamicin. The MIC of cinnamon oil, defined as the lowest concentration able to inhibit visible microbial growth, and the minimal bactericidal conce

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Villemur, Richard, Geneviève Payette, Valérie Geoffroy, Florian Mauffrey, and Christine Martineau. "Dynamics of a methanol-fed marine denitrifying biofilm: 2—impact of environmental changes on the microbial community." PeerJ 7 (August 13, 2019): e7467. http://dx.doi.org/10.7717/peerj.7467.

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Background The biofilm of a methanol-fed, marine denitrification system is composed of a multi-species microbial community, among which Hyphomicrobium nitrativorans and Methylophaga nitratireducenticrescens are the principal bacteria involved in the denitrifying activities. To assess its resilience to environmental changes, the biofilm was cultivated in artificial seawater (ASW) under anoxic conditions and exposed to a range of specific environmental conditions. We previously reported the impact of these changes on the denitrifying activities and the co-occurrence of H. nitrativorans strain NL

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Roberts, Mark E., and Philip S. Stewart. "Modelling protection from antimicrobial agents in biofilms through the formation of persister cells." Microbiology 151, no. 1 (January 1, 2005): 75–80. http://dx.doi.org/10.1099/mic.0.27385-0.

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A mathematical model of biofilm dynamics was used to investigate the protection from antimicrobial killing that could be afforded to micro-organisms in biofilms based on a mechanism of ‘persister’ cell or phenotypic variant formation. The persister state is a hypothetical, highly protected state adopted by a small fraction of the cells in a biofilm. Persisters were assumed to be generated at a fixed rate, independent of the presence of substrate or antimicrobial agent. Cells were assumed to revert from the persister state when exposed to the growth substrate. Persister cells were assumed to be

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Vieira, M. J., and L. F. Melo. "Effect of clay particles on the behaviour of biofilms formed by Pseudomonas fluorescens." Water Science and Technology 32, no. 8 (October 1, 1995): 45–52. http://dx.doi.org/10.2166/wst.1995.0260.

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This paper presents results obtained with biofilms formed under turbulent flow by a mixed suspension of Pseudomonas fluorescens and kaolin particles and compares them with biofilms formed in the absence of clay particles. The results show that the presence of kaolin particles leads to: higher accumulation of biomass in biofilm systems; higher stability of the biofilms when substrate is suppressed; higher values of the respiratory coefficient of the suspended cell cultures; increase in the mass transfer rates throughout the biofilm thickness. The results suggest that the kaolin particles enhanc

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Navarrete, Fernando, and Leonardo De La Fuente. "Response of Xylella fastidiosa to Zinc: Decreased Culturability, Increased Exopolysaccharide Production, and Formation of Resilient Biofilms under Flow Conditions." Applied and Environmental Microbiology 80, no. 3 (November 22, 2013): 1097–107. http://dx.doi.org/10.1128/aem.02998-13.

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ABSTRACTThe bacterial plant pathogenXylella fastidiosaproduces biofilm that accumulates in the host xylem vessels, affecting disease development in various crops and bacterial acquisition by insect vectors. Biofilms are sensitive to the chemical composition of the environment, and mineral elements being transported in the xylem are of special interest for this pathosystem. Here,X. fastidiosaliquid cultures were supplemented with zinc and compared with nonamended cultures to determine the effects of Zn on growth, biofilm, and exopolysaccharide (EPS) production under batch and flow culture condi

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Thomas, Vinai Chittezham, Lance R. Thurlow, Dan Boyle, and Lynn E. Hancock. "Regulation of Autolysis-Dependent Extracellular DNA Release by Enterococcus faecalis Extracellular Proteases Influences Biofilm Development." Journal of Bacteriology 190, no. 16 (June 13, 2008): 5690–98. http://dx.doi.org/10.1128/jb.00314-08.

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ABSTRACT Enterococci are major contributors of hospital-acquired infections and have emerged as important reservoirs for the dissemination of antibiotic resistance traits. The ability to form biofilms on medical devices is an important aspect of pathogenesis in the hospital environment. The Enterococcus faecalis Fsr quorum system has been shown to regulate biofilm formation through the production of gelatinase, but the mechanism has been hitherto unknown. Here we show that both gelatinase (GelE) and serine protease (SprE) contribute to biofilm formation by E. faecalis and provide clues to how

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Darmasiwi, Sari, Oktaviana Herawati, and Endah Retnaningrum. "Edible biofilm formation from guava seed waste fermentation." Digital Press Physical Sciences and Engineering 1 (2018): 00005. http://dx.doi.org/10.29037/digitalpress.11244.

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Guava seed is by-product from the consumption of guava fruits. We interested to explore further the potential of guava seed waste using fermentation method. The purpose of this research was to determine the ability of biofilm formation produced from fermentation of guava seed. Fermented guava seed was prepared by solid-state fermentation method using banana leaves wrap at 37 °C for 72 h. It were then continued with isolation and screening of bacteria from the fermentation products, preparation of bacteria cultures to be used in biofilm formation, and formation of biofilm by glass slides and br

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Verkholyuk, Mykola, Ruslan Peleno, and Iaromyr Turko. "RESISTANCE OF S. AUREUS ATCC 25923, E. COLI 055K59 No. 3912/41 AND P. AERUGINOSA 27/99 TO THE WASH-DISINFECTANT «MILKODEZ»." EUREKA: Health Sciences 1 (January 31, 2020): 55–60. http://dx.doi.org/10.21303/2504-5679.2020.001100.

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The aim of the work – the article presents the results of determining of the resistance of S. aureus ATCC 25923, E. coli 055K59 No. 3912/4 and P. aeruginosa 27/99 test cultures in planktonic form and in biofilm to our developed «Milkodez» acid detergent. Materials and methods. Microbial biofilms were grown on MPB in 5 cm disposable plastic Petri dishes. To determine the effect of disinfectants on microbial biofilms, 3 Petri dishes with biofilms of each of the test cultures were used. One of the Petri dishes served as control and she had for 15 minutes made 5 cm3 of saline NaCl solution, in the

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Webb, Jeremy S., Mathew Lau, and Staffan Kjelleberg. "Bacteriophage and Phenotypic Variation in Pseudomonas aeruginosa Biofilm Development." Journal of Bacteriology 186, no. 23 (December 1, 2004): 8066–73. http://dx.doi.org/10.1128/jb.186.23.8066-8073.2004.

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ABSTRACT A current question in biofilm research is whether biofilm-specific genetic processes can lead to differentiation in physiology and function among biofilm cells. In Pseudomonas aeruginosa, phenotypic variants which exhibit a small-colony phenotype on agar media and a markedly accelerated pattern of biofilm development compared to that of the parental strain are often isolated from biofilms. We grew P. aeruginosa biofilms in glass flow cell reactors and observed that the emergence of small-colony variants (SCVs) in the effluent runoff from the biofilms correlated with the emergence of p

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West-Barnette, Shayla, Andrea Rockel, and W. Edward Swords. "Biofilm Growth Increases Phosphorylcholine Content and Decreases Potency of Nontypeable Haemophilus influenzae Endotoxins." Infection and Immunity 74, no. 3 (March 2006): 1828–36. http://dx.doi.org/10.1128/iai.74.3.1828-1836.2006.

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ABSTRACT Nontypeable Haemophilus influenzae (NTHI) is a common respiratory commensal and opportunistic pathogen. NTHI is normally contained within the airways by host innate defenses that include recognition of bacterial endotoxins by Toll-like receptor 4 (TLR4). NTHI produces lipooligosaccharide (LOS) endotoxins which lack polymeric O side chains and which may contain host glycolipids. We recently showed that NTHI biofilms contain variants with sialylated LOS glycoforms that are essential to biofilm formation. In this study, we show that NTHI forms biofilms on epithelial cell layers. Confocal

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Dohare, Suhaga, Devendra Singh, Deepmala Sharma, and Vishnu Agarwal. "EFFECT OF Staphylococcus epidermidis ON Pseudomonas aeruginosa BIOFILM IN MIXED-SPECIES CULTURE." Journal of Experimental Biology and Agricultural Sciences 9, no. 3 (June 25, 2021): 325–34. http://dx.doi.org/10.18006/2021.9(3).325.334.

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Staphylococcus epidermidis and Pseudomonas aeruginosa, are clinically relevant pathogens that often produce biofilms. To investigate the co-survivability of S. epidermidis and P. aeruginosa in mixed cultures biofilm and planktonic form, it is important to understand more about the interspecies interaction of both species. The interspecies interaction was analyzed using streak and drop agar plate assay, cell viability assay (CFU), spectrophotometry-based method, and microscopic analysis. The findings suggest that both cells and supernatant of P. aeruginosa inhibit the planktonic growth of S. ep

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Henry-Stanley, Michelle J., Donavon J. Hess, and Carol L. Wells. "Aminoglycoside inhibition of Staphylococcus aureus biofilm formation is nutrient dependent." Journal of Medical Microbiology 63, no. 6 (June 1, 2014): 861–69. http://dx.doi.org/10.1099/jmm.0.068130-0.

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Biofilms represent microbial communities, encased in a self-produced matrix or extracellular polymeric substance. Microbial biofilms are likely responsible for a large proportion of clinically significant infections and the multicellular nature of biofilm existence has been repeatedly associated with antibiotic resistance. Classical in vitro antibiotic-susceptibility testing utilizes artificial growth media and planktonic microbes, but this method may not account for the variability inherent in environments subject to biofilm growth in vivo. Experiments were designed to test the hypothesis tha

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Koch, John A., Taylor M. Pust, Alex J. Cappellini, Jonathan B. Mandell, Dongzhu Ma, Neel B. Shah, Kimberly M. Brothers, and Kenneth L. Urish. "Staphylococcus epidermidis Biofilms Have a High Tolerance to Antibiotics in Periprosthetic Joint Infection." Life 10, no. 11 (October 24, 2020): 253. http://dx.doi.org/10.3390/life10110253.

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Both Staphylococcus aureus and Staphylococcus epidermidis are commonly associated with periprosthetic joint infections (PJIs). The treatment of PJI can be challenging because biofilms are assumed to have an increased intolerance to antibiotics. This makes the treatment of PJI challenging from a clinical perspective. Although S. aureus has been previously demonstrated to have increased biofilm antibiotic tolerance, this has not been well established with Staphylococcus epidermidis. A prospective registry of PJI S. epidermidis isolates was developed. The efficacy of clinically relevant antibioti

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Liu, Zhi, Fiona R. Stirling, and Jun Zhu. "Temporal Quorum-Sensing Induction Regulates Vibrio cholerae Biofilm Architecture." Infection and Immunity 75, no. 1 (October 30, 2006): 122–26. http://dx.doi.org/10.1128/iai.01190-06.

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ABSTRACT Vibrio cholerae, the pathogen that causes cholera, also survives in aqueous reservoirs, probably in the form of biofilms. Quorum sensing negatively regulates V. cholerae biofilm formation through HapR, whose expression is induced at a high cell density. In this study, we show that the concentration of the quorum-sensing signal molecule CAI-1 is higher in biofilms than in planktonic cultures. By measuring hapR expression and activity, we found that the induction of quorum sensing in biofilm-associated cells occurs earlier. We further demonstrate that the timing of hapR expression is cr

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Cholo, Moloko C., Sipho S. M. Rasehlo, Eudri Venter, Chantelle Venter, and Ronald Anderson. "Effects of Cigarette Smoke Condensate on Growth and Biofilm Formation by Mycobacterium tuberculosis." BioMed Research International 2020 (August 19, 2020): 1–7. http://dx.doi.org/10.1155/2020/8237402.

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Background and Objectives. Cigarette smoke (CS) is a major risk factor contributing to the burden of tuberculosis. Little is known, however, about the effects of CS exposure on growth and persistence of Mycobacterium tuberculosis (Mtb) organisms. This issue has been addressed in the current study, which is focused on the effects of cigarette smoke condensate (CSC) on the growth and viability of Mtb planktonic and biofilm-forming cultures. Materials and Methods. The planktonic and biofilm-forming cultures were prepared in Middlebrook 7H9 and Sauton broth media, respectively, using Mtb strain, H

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Maggio, Francesca, Chiara Rossi, Clemencia Chaves-López, Annalisa Serio, Luca Valbonetti, Francesco Pomilio, Alessio Pio Chiavaroli, and Antonello Paparella. "Interactions between L. monocytogenes and P. fluorescens in Dual-Species Biofilms under Simulated Dairy Processing Conditions." Proceedings 70, no. 1 (November 9, 2020): 80. http://dx.doi.org/10.3390/foods_2020-07625.

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In dairy processing environments, many bacterial species form biofilms on surfaces and equipment. Among them, Listeria monocytogenes and Pseudomonas spp. could be present in mixed-species biofilms with increased resistance to disinfectants. This study aimed to evaluate the interactions between L. monocytogenes and P. fluorescens in dual-species biofilms simulating dairy processing conditions as well as the capability of P. fluorescens to produce the blue pigment. The biofilm-forming capability of single- and mixed-cultures was evaluated on polystyrene (PS) and stainless steel (SS) surfaces at

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Ramalingam, B., R. Sekar, J. B. Boxall, and C. A. Biggs. "Aggregation and biofilm formation of bacteria isolated from domestic drinking water." Water Supply 13, no. 4 (August 1, 2013): 1016–23. http://dx.doi.org/10.2166/ws.2013.115.

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The objective of this study was to investigate the autoaggregation, coaggregation and biofilm formation of four bacteria namely Sphingobium, Xenophilus, Methylobacterium and Rhodococcus isolated from drinking water. Auto and coaggregation studies were performed by both qualitative (DAPI staining) and semi-quantitative (visual coaggregation) methods and biofilms produced by either pure or dual-cultures were quantified by crystal violet method. Results from the semi-quantitative visual aggregation method did not show any immediate auto or coaggregation, which was confirmed by the 4′,6 diamidino-

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Schafer, Mark E., and Tessie McNeely. "Combining Visible Light and Non-Focused Ultrasound Significantly Reduces Propionibacterium acnes Biofilm While Having Limited Effect on Host Cells." Microorganisms 9, no. 5 (April 26, 2021): 929. http://dx.doi.org/10.3390/microorganisms9050929.

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Bacterial biofilms are highly resistant to antibiotics and have been implicated in the etiology of 60%–80% of chronic microbial infections. We tested a novel combination of low intensity ultrasound and blue light against biofilm and planktonic bacteria. A laboratory prototype was built which produced both energies uniformly and coincidently from a single treatment head, impinging upon a 4.45 cm2 target. To demonstrate proof of concept, Propionibacterium acnes biofilms were cultured on Millicell hanging inserts in 6-well plates. Hanging inserts with biofilms were treated in a custom exposure ch

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Kuznetsova, Marina V., Irina L. Maslennikova, Tamara I. Karpunina, Larisa Yu Nesterova, and Vitaly A. Demakov. "Interactions of Pseudomonas aeruginosa in predominant biofilm or planktonic forms of existence in mixed culture with Escherichia coli in vitro." Canadian Journal of Microbiology 59, no. 9 (September 2013): 604–10. http://dx.doi.org/10.1139/cjm-2013-0168.

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Pseudomonas aeruginosa and Escherichia coli are known to be involved in mixed communities in diverse niches. In this study we examined the influence of the predominant form of cell existence of and the exometabolite production by P. aeruginosa strains on interspecies interactions, in vitro. Bacterial numbers of P. aeruginosa and E. coli in mixed plankton cultures and biofilms compared with their numbers in single plankton cultures and biofilms changed in a different way, but were in accordance with the form of P. aeruginosa cell existence. The mass of a mixed-species biofilm was greater than t

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Payette, Geneviève, Valérie Geoffroy, Christine Martineau, and Richard Villemur. "Dynamics of a methanol-fed marine denitrifying biofilm: 1-Impact of environmental changes on the denitrification and the co-occurrence of Methylophaga nitratireducenticrescens and Hyphomicrobium nitrativorans." PeerJ 7 (August 13, 2019): e7497. http://dx.doi.org/10.7717/peerj.7497.

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Background The biofilm of a methanol-fed denitrification system that treated a marine effluent is composed of multi-species microorganisms, among which Hyphomicrobium nitrativorans strain NL23 and Methylophaga nitratireducenticrescens strain JAM1 are the principal bacteria involved in the denitrifying activities. Here, we report the capacity of the denitrifying biofilm to sustain environmental changes, and the impact of these changes on the co-occurrence of H. nitrativorans and M. nitratireducenticrescens. Methods In a first set of assays, the original biofilm (OB) was cultivated in an artific

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Spoering, Amy L., and Kim Lewis. "Biofilms and Planktonic Cells of Pseudomonas aeruginosa Have Similar Resistance to Killing by Antimicrobials." Journal of Bacteriology 183, no. 23 (December 1, 2001): 6746–51. http://dx.doi.org/10.1128/jb.183.23.6746-6751.2001.

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ABSTRACT Biofilms are considered to be highly resistant to antimicrobial agents. Strictly speaking, this is not the case—biofilms do not grow in the presence of antimicrobials any better than do planktonic cells. Biofilms are indeed highly resistant to killing by bactericidal antimicrobials, compared to logarithmic-phase planktonic cells, and therefore exhibit tolerance. It is assumed that biofilms are also significantly more tolerant than stationary-phase planktonic cells. A detailed comparative examination of tolerance of biofilms versus stationary- and logarithmic-phase planktonic cells wit

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HARGREAVES, D. G., A. PAJKOS, A. K. DEVA, K. VICKERY, S. L. FILAN, and M. A. TONKIN. "The Role of Biofilm Formation in Percutaneous Kirschner-Wire Fixation of Radial Fractures." Journal of Hand Surgery 27, no. 4 (August 2002): 365–68. http://dx.doi.org/10.1054/jhsb.2002.0756.

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This study examines the formation of bacterial biofilms on percutaneous wires used for fracture fixation. Twelve control (clinically uninfected) wires and ten infected wires were collected and examined using broth culture and scanning electron microscopy. Three of the 12 control wires grew Staphylococcus spp. with very low bacterial counts in their percutaneous portions. In the clinically infected wires, six wires in four subjects had positive cultures in their percutaneous portions and four of these also had positive cultures in their deep portions with much higher bacterial counts than the c

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Elkins, James G., Daniel J. Hassett, Philip S. Stewart, Herbert P. Schweizer, and Timothy R. McDermott. "Protective Role of Catalase in Pseudomonas aeruginosa Biofilm Resistance to Hydrogen Peroxide." Applied and Environmental Microbiology 65, no. 10 (October 1, 1999): 4594–600. http://dx.doi.org/10.1128/aem.65.10.4594-4600.1999.

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ABSTRACT The role of the two known catalases in Pseudomonas aeruginosa in protecting planktonic and biofilm cells against hydrogen peroxide (H2O2) was investigated. Planktonic cultures and biofilms formed by the wild-type strain PAO1 and the katA and katB catalase mutants were compared for their susceptibility to H2O2. Over the course of 1 h, wild-type cell viability decreased steadily in planktonic cells exposed to a single dose of 50 mM H2O2, whereas biofilm cell viability remained at approximately 90% when cells were exposed to a flowing stream of 50 mM H2O2. The katB mutant, lacking the H2

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Saginur, Raphael, Melissa StDenis, Wendy Ferris, Shawn D. Aaron, Francis Chan, Craig Lee, and Karam Ramotar. "Multiple Combination Bactericidal Testing of Staphylococcal Biofilms from Implant-Associated Infections." Antimicrobial Agents and Chemotherapy 50, no. 1 (January 2006): 55–61. http://dx.doi.org/10.1128/aac.50.1.55-61.2006.

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ABSTRACT Standardized susceptibility testing fails to predict in vivo resistance of device-related infections to antimicrobials. We assessed agents and combinations of antimicrobials against clinical isolates of Staphylococcus epidermidis and S. aureus (methicillin-resistant S. aureus and methicillin-sensitive S. aureus) retrieved from device-associated infections. Isolates were grown planktonically and as biofilms. Biofilm cultures of the organisms were found to be much more resistant to inhibitory and bactericidal effects of single and combination antibiotics than planktonic cultures (P <

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Zannier, Federico, Luciano Raúl Portero, Omar Federico Ordoñez, Luciano José Martinez, María Eugenia Farías, and Virginia Helena Albarracin. "Polyextremophilic Bacteria from High Altitude Andean Lakes: Arsenic Resistance Profiles and Biofilm Production." BioMed Research International 2019 (February 21, 2019): 1–11. http://dx.doi.org/10.1155/2019/1231975.

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High levels of arsenic present in the High Altitude Andean Lakes (HAALs) ecosystems selected arsenic-resistant microbial communities which are of novel interest to study adaptations mechanisms potentially useful in bioremediation processes. We herein performed a detailed characterization of the arsenic tolerance profiles and the biofilm production of two HAAL polyextremophiles, Acinetobacter sp. Ver3 (Ver3) and Exiguobacterium sp. S17 (S17). Cellular adherence over glass and polypropylene surfaces were evaluated together with the effect of increasing doses and oxidative states of arsenic over

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Niemira, Brendan A., and Ethan B. Solomon. "Sensitivity of Planktonic and Biofilm-Associated Salmonella spp. to Ionizing Radiation." Applied and Environmental Microbiology 71, no. 5 (May 2005): 2732–36. http://dx.doi.org/10.1128/aem.71.5.2732-2736.2005.

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ABSTRACT Salmonella enterica forms biofilms that are relatively resistant to chemical sanitizing treatments. Ionizing radiation has been used to inactivate Salmonella on a variety of foods and contact surfaces, but the relative efficacy of the process against biofilm-associated cells versus free-living planktonic cells is not well documented. The radiation sensitivity of planktonic or biofilm-associated cells was determined for three food-borne-illness-associated isolates of Salmonella. Biofilms were formed on sterile glass slides in a coincubation apparatus, using inoculated tryptic soy broth

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Maggio, Francesca, Chiara Rossi, Clemencia Chaves-López, Annalisa Serio, Luca Valbonetti, Francesco Pomilio, Alessio Pio Chiavaroli, and Antonello Paparella. "Interactions between L. monocytogenes and P. fluorescens in Dual-Species Biofilms under Simulated Dairy Processing Conditions." Foods 10, no. 1 (January 16, 2021): 176. http://dx.doi.org/10.3390/foods10010176.

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In dairy processing environments, many bacterial species adhere and form biofilms on surfaces and equipment, leading to foodborne illness and food spoilage. Among them, Listeria monocytogenes and Pseudomonas spp. could be present in mixed-species biofilms. This study aimed to evaluate the interactions between L. monocytogenes and P. fluorescens in biofilms simulating dairy processing conditions, as well as the capability of P. fluorescens in co-culture to produce the blue pigment in a Ricotta-based model system. The biofilm-forming capability of single- and mixed-cultures was evaluated on poly

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Valieva, R. I., S. A. Lisovskaya, K. A. Mayanskaya, D. V. Samigullin, and G. Sh Isaeva. "Features of antifungal therapy during long-lasting infectious process: a clinical case of fungal keratitis and profile of antifungal sensitivity based on assessing biofilm formation." Russian Journal of Infection and Immunity 11, no. 4 (September 20, 2021): 789–97. http://dx.doi.org/10.15789/2220-7619-foa-1495.

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Among infectious diseases, opportunistic mycoses hold a special place. There has been accumulating a lot of evidence regarding the clinical and epidemiological aspects of infection caused by Fusarium spp., which global incidence rate among microbial keratitis ranges from 2 to 40% depending on the geographical location of the country. Colonizing mucous membranes, fungi can exist not only in the form of plankton, but form biofilms after surface attachment, which leads to elevated resistance to multiple antifungal agents. Here we describe a clinical case of fungal keratitis due to Fusarium solani

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Hunt, Stephen M., Erin M. Werner, Baochuan Huang, Martin A. Hamilton, and Philip S. Stewart. "Hypothesis for the Role of Nutrient Starvation in Biofilm Detachment." Applied and Environmental Microbiology 70, no. 12 (December 2004): 7418–25. http://dx.doi.org/10.1128/aem.70.12.7418-7425.2004.

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ABSTRACT A combination of experimental and theoretical approaches was used to investigate the role of nutrient starvation as a potential trigger for biofilm detachment. Experimental observations of detachment in a variety of biofilm systems were made with pure cultures of Pseudomonas aeruginosa. These observations indicated that biofilms grown under continuous-flow conditions detached after flow was stopped, that hollow cell clusters were sometimes observed in biofilms grown in flow cells, and that lysed cells were apparent in the internal strata of colony biofilms. When biofilms were nutrient

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Gerner, Erik, Sofia Almqvist, Peter Thomsen, Maria Werthén, and Margarita Trobos. "Sodium Salicylate Influences the Pseudomonas aeruginosa Biofilm Structure and Susceptibility Towards Silver." International Journal of Molecular Sciences 22, no. 3 (January 21, 2021): 1060. http://dx.doi.org/10.3390/ijms22031060.

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Hard-to-heal wounds are typically infected with biofilm-producing microorganisms, such as Pseudomonas aeruginosa, which strongly contribute to delayed healing. Due to the global challenge of antimicrobial resistance, alternative treatment strategies are needed. Here, we investigated whether inhibition of quorum sensing (QS) by sodium salicylate in different P. aeruginosa strains (QS-competent, QS-mutant, and chronic wound strains) influences biofilm formation and tolerance to silver. Biofilm formation was evaluated in simulated serum-containing wound fluid in the presence or absence of sodium

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Cushion, Melanie T., and Margaret S. Collins. "Susceptibility of Pneumocystis to Echinocandins in Suspension and Biofilm Cultures." Antimicrobial Agents and Chemotherapy 55, no. 10 (July 25, 2011): 4513–18. http://dx.doi.org/10.1128/aac.00017-11.

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ABSTRACTThe targeted inhibition of cyst but not trophic development by anidulafungin, caspofungin, and micafungin onPneumocystis murinaandPneumocystis cariniiin rodent models ofPneumocystis cariniipneumonia (PCP) was recently reported by us (M. T. Cushion et al., PLoS One 5:e8524, 2010). To better understand the effects of echinocandins onP. carinii, the same three compounds were evaluated in standard suspension and biofilm cultures supplemented with various concentrations of sera using the measurement of ATP as the indicator. In suspension cultures with 1 and 5% serum, anidulafungin was the m

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Hathroubi, Skander, Francis Beaudry, Chantale Provost, Léa Martelet, Mariela Segura, Carl A. Gagnon, and Mario Jacques. "Impact ofActinobacillus pleuropneumoniaebiofilm mode of growth on the lipid A structures and stimulation of immune cells." Innate Immunity 22, no. 5 (May 25, 2016): 353–62. http://dx.doi.org/10.1177/1753425916649676.

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Actinobacillus pleuropneumoniae (APP), the etiologic agent of porcine pleuropneumonia, forms biofilms on biotic and abiotic surfaces. APP biofilms confers resistance to antibiotics. To our knowledge, no studies have examined the role of APP biofilm in immune evasion and infection persistence. This study was undertaken to (i) investigate biofilm-associated LPS modifications occurring during the switch to biofilm mode of growth; and (ii) characterize pro-inflammatory cytokines expression in porcine pulmonary alveolar macrophages (PAMs) and proliferation in porcine PBMCs challenged with planktoni

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Wijesinghe, Gayan, Ayomi Dilhari, Buddhika Gayani, Nilwala Kottegoda, Lakshman Samaranayake, and Manjula Weerasekera. "Influence of Laboratory Culture Media on in vitro Growth, Adhesion, and Biofilm Formation of Pseudomonas aeruginosa and Staphylococcus aureus." Medical Principles and Practice 28, no. 1 (October 23, 2018): 28–35. http://dx.doi.org/10.1159/000494757.

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Objective: Pseudomonas aeruginosa and Staphylococcus aureus dual-species biofilm infections are notoriously difficult to manage. This study aimed at investigating the influence of four different culture media on the planktonic growth, adhesion, and biofilm formation of P. aeruginosa and S. aureus. Materials and Methods: We monitored four different culture media including Nutrient Broth, Brain Heart Infusion (BHI) broth, Luria-Bertani broth, and RPMI 1640 medium on the planktonic growth, adhesion, and biofilm formation of P. aeruginosa (ATCC 27853) and S. aureus (ATCC 25923) using MTT assay and

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Ghani, M., and J. S. Soothill. "Ceftazidime, gentamicin, and rifampicin, in combination, kill biofilms of mucoidPseudomonas aeruginosa." Canadian Journal of Microbiology 43, no. 11 (November 1, 1997): 999–1004. http://dx.doi.org/10.1139/m97-144.

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In continuous flow biofilm cultures in medium resembling cystic fibrosis bronchial secretions, Pseudomonas aeruginosa was not eradicated from biofilms by 1 week of treatment with high concentrations of ceftazidime and gentamicin, to which the strains were sensitive on conventional testing. The addition of rifampicin, which has little activity against the strains as measured by the minimum inhibitory concentration, led to the apparent elimination of the bacteria from the biofilms. The effect was not strain specific.Key words: Pseudomonas aeruginosa, biofilm, rifampicin.

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Kononenko, A. B., D. A. Bannikova, I. B. Pavlova, S. V. Britova, and E. P. Savinova. "FORMATION OF BIOLOGICAL FILMS OF MICROORGANISMS ON VARIOUS SURFACES OF THE ENVIRONMENT." Problems of Veterinary Sanitation, Hygiene and Ecology 1, no. 3 (2020): 333–40. http://dx.doi.org/10.36871/vet.san.hyg.ecol.202003008.

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The article presents material on the results of experimental studies on the formation of biofilms by microorganisms of individual taxonomic groups on various surfaces of objects of the environment. The time and degree of biofilm formation, as well as the dependence on the type of surface and bacteria, were established. The cultures of the genera Salmonella, E. coli, Yersinia, Proteus, Pseudomonas and Staphylococcus were used in the work. To study the formation of biofilms, 24-hour cultures of S-form microorganisms grown on solid or in liquid nutrient media were used. Test objects were stained

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Geoffroy, Valérie, Geneviève Payette, Florian Mauffrey, Livie Lestin, Philippe Constant, and Richard Villemur. "Strain-level genetic diversity ofMethylophaga nitratireducenticrescensconfers plasticity to denitrification capacity in a methylotrophic marine denitrifying biofilm." PeerJ 6 (April 23, 2018): e4679. http://dx.doi.org/10.7717/peerj.4679.

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BackgroundThe biofilm of a methanol-fed, fluidized denitrification system treating a marine effluent is composed of multi-species microorganisms, among whichHyphomicrobium nitrativoransNL23 andMethylophaga nitratireducenticrescensJAM1 are the principal bacteria involved in the denitrifying activities. Strain NL23 can carry complete nitrate (NO${}_{3}^{-}$) reduction to N2, whereas strain JAM1 can perform 3 out of the 4 reduction steps. A small proportion of other denitrifiers exists in the biofilm, suggesting the potential plasticity of the biofilm in adapting to environmental changes. Here, w

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