Literatura académica sobre el tema "Calcium transient duration"

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Artículos de revistas sobre el tema "Calcium transient duration"

1

Ratan, R. R., F. R. Maxfield, and M. L. Shelanski. "Long-lasting and rapid calcium changes during mitosis." Journal of Cell Biology 107, no. 3 (1988): 993–99. http://dx.doi.org/10.1083/jcb.107.3.993.

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A more complete understanding of calcium's role in cell division requires knowledge of the timing, magnitude, and duration of changes in cytoplasmic-free calcium, [Ca2+]i, associated with specific mitotic events. To define the temporal relationship of changes in [Ca2+]i to cellular and chromosomal movements, we have measured [Ca2+]i every 6-7 s in single-dividing Pt K2 cells using fura-2 and microspectrophotometry, coupling each calcium measurement with a bright-field observation. In the 12 min before discernable chromosome some separation, 90% of metaphase cells show at least one transient of
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2

Liu, Y., S. L. Carroll, M. G. Klein, and M. F. Schneider. "Calcium transients and calcium homeostasis in adult mouse fast-twitch skeletal muscle fibers in culture." American Journal of Physiology-Cell Physiology 272, no. 6 (1997): C1919—C1927. http://dx.doi.org/10.1152/ajpcell.1997.272.6.c1919.

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Skeletal muscle fibers enzymatically dissociated from adult mouse flexor digitorum brevis muscles were maintained in culture for up to 8 days. After various times in culture, fibers were loaded with fura 2, and Ca2+ transients for trains of 1, 5, and 10 action potentials (100 Hz) triggered by external electrical stimulation were calculated from fluorescence ratio records corrected for noninstantaneous reaction of fura 2 with Ca2+. The decay rate constants of Ca2+ transients decreased with increasing stimulation duration, indicating a slowing of the Ca(2+)-removal properties with increased stim
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3

Vyshedskiy, Andrey, and Jen-Wei Lin. "Presynaptic Ca2+ Influx at the Inhibitor of the Crayfish Neuromuscular Junction: A Photometric Study at a High Time Resolution." Journal of Neurophysiology 83, no. 1 (2000): 552–62. http://dx.doi.org/10.1152/jn.2000.83.1.552.

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Presynaptic calcium influx at the inhibitor of the crayfish neuromuscular junction was investigated by measuring fluorescence transients generated by calcium-sensitive dyes. This approach allowed us to correlate presynaptic calcium influx with transmitter release at a high time resolution. Systematic testing of the calcium indicators showed that only low-affinity dyes, with affinities in the range of micromolar, should be used to avoid saturation of dye binding and interference with transmitter release. Presynaptic calcium influx was regulated by slowly increasing the duration of the action po
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4

Gidö, Gunilla, Kenichiro Katsura, Tibor Kristian, and Bo K. Siesjö. "Influence of Plasma Glucose Concentration on Rat Brain Extracellular Calcium Transients during Spreading Depression." Journal of Cerebral Blood Flow & Metabolism 13, no. 1 (1993): 179–82. http://dx.doi.org/10.1038/jcbfm.1993.21.

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The objective of this study was to establish whether tissues that are energy compromised, but not energy depleted, demonstrate exaggerated calcium transients when subjected to membrane depolarizations of the spreading depression (SD) type. Anesthetized and artificially ventilated rats were given insulin in order to induce progressively lower plasma glucose concentrations. Spreading depression was elicited by local application of KCl; extracellular calcium concentration (Ca2+e) as well as direct current (DC) potential were recorded. When plasma glucose concentration fell below ∼3 m M, the durat
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5

Wang, C., and Z. Machaty. "189 CHARACTERIZATION OF THE FIRST SPERM-INDUCED CALCIUM TRANSIENT IN PIG OOCYTES." Reproduction, Fertility and Development 28, no. 2 (2016): 225. http://dx.doi.org/10.1071/rdv28n2ab189.

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Fertilization in mammals is associated with repetitive elevations in the oocytes’ intracellular free calcium concentration. The elevations are triggered by the fertilizing sperm and are responsible for stimulating embryo development. In mouse oocytes, the sperm-induced calcium signal starts with a calcium rise that is larger and longer in duration than any succeeding transients. It also has unique characteristics: it begins with a rapid increase for 2–3 s followed by a shoulder, which is an inflection point that represents a brief decline in the rise of calcium levels. Once calcium level reach
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6

Hartmann, T., M. Kondo, H. Mochizuki, A. S. Verkman, and J. H. Widdicombe. "Calcium-dependent regulation of Cl secretion in tracheal epithelium." American Journal of Physiology-Lung Cellular and Molecular Physiology 262, no. 2 (1992): L163—L168. http://dx.doi.org/10.1152/ajplung.1992.262.2.l163.

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In primary cultures of dog tracheal epithelium, isoproterenol produced a transient increase in short-circuit current (Isc) (duration 30 s; maximal increase, 32 +/- 5 microA/cm2). This was followed by a more slowly developing sustained increase (9 +/- 3 microA/cm2), which mimicked the response to N6, 2'-O-dibutyryladenosine 3',5'-cyclic monophosphate (DBcAMP). The transient and sustained responses had dissociation constants for isoproterenol of 2 x 10(-8) and 2 x 10(-9) M, respectively. Bradykinin (in the presence of indomethacin), substance P, histamine, and thrombin produced only transient in
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7

Chaigne, Sebastien, Guillaume Cardouat, Julien Louradour, et al. "Transient receptor potential vanilloid 4 channel participates in mouse ventricular electrical activity." American Journal of Physiology-Heart and Circulatory Physiology 320, no. 3 (2021): H1156—H1169. http://dx.doi.org/10.1152/ajpheart.00497.2020.

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Transient receptor potential vanilloid 4 (TRPV4) is expressed at the membrane of mouse ventricular myocytes and colocalizes with non-T-tubular L-type calcium channels. Deletion of trpv4 gene in mice results in shortened QT interval on electrocardiogram and reduced action potential duration of ventricular myocytes. Pharmacological activation of TRPV4 channel leads to increased action potential duration and increased calcium transient amplitude in trpv4−/− but not in trpv4−/− ventricular myocytes. To the contrary, TRPV4 channel pharmacological inhibition reduces action potential duration in trpv
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8

Schneider, Eve R., Eugene F. Civillico, and Samuel S. H. Wang. "Calcium-based dendritic excitability and its regulation in the deep cerebellar nuclei." Journal of Neurophysiology 109, no. 9 (2013): 2282–92. http://dx.doi.org/10.1152/jn.00925.2012.

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The deep cerebellar nuclei (DCN) convey the final output of the cerebellum and are a major site of activity-dependent plasticity. Here, using patch-clamp recording and two-photon calcium imaging in rat brain slices, we demonstrate that DCN dendrites exhibit three hallmarks of active amplification of electrical signals. First, they produce calcium transients with rise times of tens of milliseconds, comparable in amplitude and duration to calcium spikes in other neurons. Second, calcium signal amplitudes are undiminished along the length of dendrites to the farthest distances from the soma. Thir
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9

Roome, Chris J., Emmet M. Power, and Ruth M. Empson. "Transient reversal of the sodium/calcium exchanger boosts presynaptic calcium and synaptic transmission at a cerebellar synapse." Journal of Neurophysiology 109, no. 6 (2013): 1669–80. http://dx.doi.org/10.1152/jn.00854.2012.

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The sodium/calcium exchanger (NCX) is a widespread transporter that exchanges sodium and calcium ions across excitable membranes. Normally, NCX mainly operates in its “forward” mode, harnessing the electrochemical gradient of sodium ions to expel calcium. During membrane depolarization or elevated internal sodium levels, NCX can instead switch the direction of net flux to expel sodium and allow calcium entry. Such “reverse”-mode NCX operation is frequently implicated during pathological or artificially extended periods of depolarization, not during normal activity. We have used fast calcium im
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10

Katra, Rodolphe P., Etienne Pruvot, and Kenneth R. Laurita. "Intracellular calcium handling heterogeneities in intact guinea pig hearts." American Journal of Physiology-Heart and Circulatory Physiology 286, no. 2 (2004): H648—H656. http://dx.doi.org/10.1152/ajpheart.00374.2003.

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Regional heterogeneities of ventricular repolarizing currents and their role in arrhythmogenesis have received much attention; however, relatively little is known regarding heterogeneities of intracellular calcium handling. Because repolarization properties and contractile function are heterogeneous from base to apex of the intact heart, we hypothesize that calcium handling is also heterogeneous from base to apex. To test this hypothesis, we developed a novel ratiometric optical mapping system capable of measuring calcium fluorescence of indo-1 at two separate wavelengths from 256 sites simult
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