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Artículos de revistas sobre el tema "Chimeric competency"

Autor: Grafiati
Publicado: 21 de diciembre de 2024
Última modificación: 31 de julio de 2025

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1

Strell, Phoebe, Anala Shetty, Clifford J. Steer, and Walter C. Low. "Interspecies Chimeric Barriers for Generating Exogenic Organs and Cells for Transplantation." Cell Transplantation 31 (January 2022): 096368972211105. http://dx.doi.org/10.1177/09636897221110525.

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A growing need for organs and novel cell-based therapies has provided a niche for approaches like interspecies chimeras. To generate organs from one donor species in another host species requires techniques such as blastocyst complementation and gene editing to successfully create an embryo that has cells from both the donor and the host. However, the task of developing highly efficacious and competent interspecies chimeras is met by many challenges. These interspecies chimeric barriers impede the formation of chimeras, often leading to lower levels of chimeric competency. The barriers that ne
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2

Lacadena Calero, Juan Ramón. "BIOÉTIC AS MACAQUE-HUMAN CHIMERAS: SCIENTIFIC ASPECTS AND BIOETHICAL REFLECTIONS." Anales de la Real Academia Nacional de Farmacia, no. 87(02) (2021): 117–21. http://dx.doi.org/10.53519/anaesranf.2021.87.02.01.

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The obtention by Izpisua and collaborators (2021) of macaque-human chimeric embryos by microinjection of human pluripotent stem cells into early blastocysts of cynomolgus monkey is described. They studied the competency of human pluripotent stem cells in macaque embryos cultured ex vivo until 19 days post-fertilization. A reflection on these experiments is made from the bioethical point of view.
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3

Afanassieff, Marielle, Florence Perold, Wilhelm Bouchereau, Antoine Cadiou, and Nathalie Beaujean. "Embryo-derived and induced pluripotent stem cells: Towards naive pluripotency and chimeric competency in rabbits." Experimental Cell Research 389, no. 2 (2020): 111908. http://dx.doi.org/10.1016/j.yexcr.2020.111908.

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4

Hirabayashi, M., T. Goto, C. Tamura, M. Sanbo, and S. Hochi. "202 EFFECT OF LEUKEMIA INHIBITORY FACTOR AND FORSKOLIN ON ESTABLISHMENT OF RAT EMBRYONIC STEM CELL LINES." Reproduction, Fertility and Development 26, no. 1 (2014): 215. http://dx.doi.org/10.1071/rdv26n1ab202.

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Rat embryonic stem (ES) cell lines can be established in culture medium containing inhibitors for glycogen synthase kinase 3 (GSK3) and mitogen-activated protein kinase (MEK). We confirmed reproducibility of the 2i culture system in establishing rat ES cell lines (Hirabayashi et al. 2010 Mol. Reprod. Dev. 77, 94) and the likelihood of successful germline transmission (genuine) of rat ES cell lines established in leukemia inhibitory factor (LIF)- and forskolin (FK)-supplemented 2i medium (Hirabayashi et al. 2013 Transgenic Res. 22, 411–416). This study was designed to investigate whether LIF an
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5

Tapponnier, Yann, Marielle Afanassieff, Irène Aksoy, et al. "Reprogramming of rabbit induced pluripotent stem cells toward epiblast and chimeric competency using Krüppel-like factors." Stem Cell Research 24 (October 2017): 106–17. http://dx.doi.org/10.1016/j.scr.2017.09.001.

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6

Whitaker, Neal, Trista M. Berry, Nathan Rosenthal, et al. "Chimeric Coupling Proteins Mediate Transfer of Heterologous Type IV Effectors through the Escherichia coli pKM101-Encoded Conjugation Machine." Journal of Bacteriology 198, no. 19 (2016): 2701–18. http://dx.doi.org/10.1128/jb.00378-16.

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ABSTRACTBacterial type IV secretion systems (T4SSs) are composed of two major subfamilies, conjugation machines dedicated to DNA transfer and effector translocators for protein transfer. We show here that theEscherichia colipKM101-encoded conjugation system, coupled with chimeric substrate receptors, can be repurposed for transfer of heterologous effector proteins. The chimeric receptors were composed of the N-terminal transmembrane domain of pKM101-encoded TraJ fused to soluble domains of VirD4 homologs functioning inAgrobacterium tumefaciens,Anaplasma phagocytophilum, orWolbachia pipientis.
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7

Kondoh, Gen, Yoichi Yamamoto, Kayo Yoshida, et al. "Easy assessment of ES cell clone potency for chimeric development and germ-line competency by an optimized aggregation method." Journal of Biochemical and Biophysical Methods 39, no. 3 (1999): 137–42. http://dx.doi.org/10.1016/s0165-022x(99)00008-1.

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8

Fields, Chris, and Michael Levin. "Competency in Navigating Arbitrary Spaces as an Invariant for Analyzing Cognition in Diverse Embodiments." Entropy 24, no. 6 (2022): 819. http://dx.doi.org/10.3390/e24060819.

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One of the most salient features of life is its capacity to handle novelty and namely to thrive and adapt to new circumstances and changes in both the environment and internal components. An understanding of this capacity is central to several fields: the evolution of form and function, the design of effective strategies for biomedicine, and the creation of novel life forms via chimeric and bioengineering technologies. Here, we review instructive examples of living organisms solving diverse problems and propose competent navigation in arbitrary spaces as an invariant for thinking about the sca
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9

Zaslavsky, Alexander, Mackenzie Adams, Sandra Wissmueller, et al. "Glypican-1 as a novel immunotherapeutic target in prostate cancer." Journal of Clinical Oncology 36, no. 6_suppl (2018): 174. http://dx.doi.org/10.1200/jco.2018.36.6_suppl.174.

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174 Background: New effective therapies for men with prostate cancer are desperately needed. Recently, cancer immunotherapy has emerged as an important new treatment strategy for prostate cancer and for castrate resistant prostate cancer (CRPC). Multiple studies have identified the heparan sulfate proteoglycan-1 Glypican 1 (GPC-1) as being overexpressed in different cancers, and also as being a possible marker of poor prognosis in several solid tumor cancers. GPC-1 has been recently identified as a potential marker for prostate cancer. The MIL-38 monoclonal antibody detects GPC-1 and an IgG1 c
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10

Ugale, Amol Sanjay, Gudmundur Logi Norddahl, Martin Wahlestedt, et al. "Hematopoietic Stem Cells Are Intrinsically Protected Against MLL-ENL Mediated Transformation." Blood 124, no. 21 (2014): 839. http://dx.doi.org/10.1182/blood.v124.21.839.839.

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Abstract Studies on the developmental pathways of hematopoietic stem cells (HSCs) have led to roadmaps of differentiation and resulted in key information concerning lineage relationships and restriction points in the blood system. This knowledge is also central to understand the etiology of acute myeloid leukemia (AML), where recent work has proposed that the heterogeneity and aggressiveness of AML can associate with the developmental stage of transformation. Balanced chromosomal translocations that result in fusion proteins with aberrant transcriptional regulatory activities are frequent init
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11

Nakano, K., M. Watanabe, H. Matsunari, et al. "297 PRODUCTION OF CHIMERIC PORCINE FETUSES BY AGGREGATION METHOD USING PARTHENOGENETIC EMBRYOS." Reproduction, Fertility and Development 25, no. 1 (2013): 296. http://dx.doi.org/10.1071/rdv25n1ab297.

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Porcine induced pluripotent stem (iPS) cells are considered to be an invaluable research tool in translational research with pigs as a large animal model. Pluripotency of the iPS cells needs to be verified by their competence to contribute to chimera formation. The aim of the present study is to establish feasible system to create chimeric pig fetuses using parthenogenetic embryos. In Experiment 1, inner cell mass (ICM) was isolated by immunosurgery from Day 6 blastocysts obtained by parthenogenetic activation of in vitro matured (IVM) oocytes. Isolated ICM were used as the donor cells after s
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12

Matsunari, H., K. Nakano, T. Kanai, et al. "26 IN VIVO EXOGENIC ORGAN GENERATION WITH ORGANOGENESIS-DISABLED CLONED PIGS AS A PLATFORM." Reproduction, Fertility and Development 26, no. 1 (2014): 127. http://dx.doi.org/10.1071/rdv26n1ab26.

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The generation of organs from pluripotent stem cells (PSC) is one of the ultimate goals of regenerative medicine. We have demonstrated that functional organs can be generated in vivo from xenogenic PSC in the body of organogenesis-disabled mice using blastocyst complementation. To apply this principle in generating human organs, a technical platform using large non-rodent mammals is essential. The aim of the present study was to establish a blastocyst complementation system using cloned pig embryos. We generated transgenic-cloned pigs with an apancreatic phenotype via the overexpression of Hes
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13

Wei, Hairong, and Michael Brown. "MHC Class I Dk expression in hematopoietic and non-hematopoietic cells is essential to NK cell licensing and murine CMV resistance (P6308)." Journal of Immunology 190, no. 1_Supplement (2013): 182.6. http://dx.doi.org/10.4049/jimmunol.190.supp.182.6.

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Abstract Ly49G2 inhibitory receptor-bearing NK cells are essential to viral control in murine (M)CMV infected MA/My and MHC class I Dk-expressing C57L mice. In bone marrow chimeric (BMT) mice, highly effective NK cell-mediated viral control requires Dk expression in both hematopoietic and non-hematopoietic cells. Because Dk is a licensing ligand for Ly49G2, we reasoned that Dk expression in discrete cell lineages modulates NK responsiveness and viral control. To address the question, we generated additional BMT mice and measured the effect of Dk expression on NK effector competency and virus c
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14

Haqshenas, G., X. Dong, H. Netter, J. Torresi, and E. J. Gowans. "A chimeric GB virus B encoding the hepatitis C virus hypervariable region 1 is infectious in vivo." Journal of General Virology 88, no. 3 (2007): 895–902. http://dx.doi.org/10.1099/vir.0.82467-0.

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Two GB virus B (GBV-B) chimeric genomes, GBV-HVR and GBV-HVRh (with a hinge), containing the coding region of the immunodominant hypervariable region 1 (HVR1) of the E2 envelope protein of Hepatitis C virus (HCV) were constructed. Immunoblot analysis confirmed that HVR1 was anchored to the GBV-B E2 protein. To investigate the replication competence and in vivo stability of in vitro-generated chimeric RNA transcripts, two naïve marmosets were inoculated intrahepatically with the transcripts. The GBV-HVR chimeric genome was detectable for 2 weeks post-inoculation (p.i.), whereas GBV-HVRh reverte
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15

Kim, Kee-Pyo, You Wu, Juyong Yoon, et al. "Reprogramming competence of OCT factors is determined by transactivation domains." Science Advances 6, no. 36 (2020): eaaz7364. http://dx.doi.org/10.1126/sciadv.aaz7364.

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OCT4 (also known as POU5F1) plays an essential role in reprogramming. It is the only member of the POU (Pit-Oct-Unc) family of transcription factors that can induce pluripotency despite sharing high structural similarities to all other members. Here, we discover that OCT6 (also known as POU3F1) can elicit reprogramming specifically in human cells. OCT6-based reprogramming does not alter the mesenchymal-epithelial transition but is attenuated through the delayed activation of the pluripotency network in comparison with OCT4-based reprogramming. Creating a series of reciprocal domain-swapped chi
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16

Geiger, B., D. Salomon, M. Takeichi, and R. O. Hynes. "A chimeric N-cadherin/beta 1-integrin receptor which localizes to both cell-cell and cell-matrix adhesions." Journal of Cell Science 103, no. 4 (1992): 943–51. http://dx.doi.org/10.1242/jcs.103.4.943.

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To study the molecular mechanisms involved in formation of cell contacts, we have transfected cultured cells with a chimeric cDNA encoding the cytoplasmic and transmembrane domains of beta 1 integrin and the extracellular region of N-cadherin and determined the subcellular distribution of the chimeric molecule. We show that the chimeric receptor associates preferentially with cell-matrix focal contacts, suggesting that its distribution is directed by its beta 1 integrin segment, presumably via interactions of the cytoplasmic domain with cytoskeletal elements characteristic of focal contacts. T
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17

Sánchez-Moguel, Ignacio, Carmina Montiel, and Ismael Bustos-Jaimes. "Therapeutic Potential of Engineered Virus-like Particles of Parvovirus B19." Pathogens 12, no. 8 (2023): 1007. http://dx.doi.org/10.3390/pathogens12081007.

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Virus-like particles (VLPs) comprise one or many structural components of virions, except their genetic material. Thus, VLPs keep their structural properties of cellular recognition while being non-infectious. VLPs of Parvovirus B19 (B19V) can be produced by the heterologous expression of their structural proteins VP1 and VP2 in bacteria. These proteins are purified under denaturing conditions, refolded, and assembled into VLPs. Moreover, chimeric forms of VP2 have been constructed to harbor peptides or functional proteins on the surface of the particles without dropping their competence to fo
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18

Leventhal, Joseph, Larry D. Bozulic, Mark D. Badder, et al. "Evaluation Of Immunocompentence In Tolerant Chimeric Recipients Of Hematopoietic Stem Cell/Renal Transplants." Blood 122, no. 21 (2013): 4483. http://dx.doi.org/10.1182/blood.v122.21.4483.4483.

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A phase 2 protocol was developed to attempt to induce donor-specific tolerance to renal allografts in related and unrelated donor/recipient combinations (IND 13947 phase 2). Subjects were conditioned with fludarabine (days -5, -4, -3), cyclophosphamide (50 mg/ky days -3 and +3), and 200 cGy total body irradiation. The kidney transplant was performed day 0. G-CSF mobilized peripheral blood stem cells processed to remove GVHD-producing cells and retain graft facilitating cells (FC) was administered on day +1. The conditioning was well tolerated and the subjects were managed as outpatients after
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19

Matatall, Katie, Ching-Chieh Shen, Yayun Zheng, and Katherine Y. King. "Chronic Mycobacterium Avium Infection Leads to Cell Autonomous Exhaustion of Hematopoietic Stem Cells." Blood 124, no. 21 (2014): 2947. http://dx.doi.org/10.1182/blood.v124.21.2947.2947.

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Abstract Background: Chronic infections and inflammatory conditions can cause bone marrow suppression and pancytopenia, a dangerous condition that impairs recovery from infection. In some chronic infections such as tuberculosis, marrow suppression has been ascribed to myelofibrosis. However, inflammatory conditions also affect hematopoietic stem cells (HSCs) themselves. HSCs are activated to proliferate and differentiate in the setting of a Mycobacterium avium infection. These responses are mediated by interferon gamma (IFNg) signaling. We hypothesized that sustained interferon signaling durin
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20

Willis, Lauren, Sara R. Fagerlie, and Sattva S. Neelapu. "Evaluating Hematologist's Knowledge of CAR T-Cell Therapy in Hematologic Malignancies." Blood 132, Supplement 1 (2018): 2269. http://dx.doi.org/10.1182/blood-2018-99-115036.

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Abstract Background: The objective of this study was to assess current clinical practices of hematologist/oncologist (hem/onc) specialists related to chimeric antigen receptor (CAR) T-cell therapy in hematologic malignancies, in order to identify knowledge, competency, and practice gaps and barriers to optimal care. Methods: A continuing medical education (CME)-certified clinical practice assessment consisting of 25 multiple choice questions was developed to measure knowledge, skills, attitudes, and competence of hem/onc specialists regarding CAR T-cell therapy. The survey instrument was made
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21

Gatenbee, Chandler D., Mark Robertson-Tessi, Maximilian Strobl, et al. "Abstract A011: Modeling the coevolution of native and CAR T-cells in large B cell lymphoma reveals a potential biomarker for response to therapy." Cancer Research 84, no. 3_Supplement_2 (2024): A011. http://dx.doi.org/10.1158/1538-7445.canevol23-a011.

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Abstract Application of CD19 targeted Chimeric Antigen Receptor (CAR) T-cell therapy to large B cell lymphoma (LBCL) has yielded unprecedented clinical outcomes in a cancer largely resistant to conventional chemotherapy. This is highlighted in the recent ZUMA-7 trial, where axicabtagene ciloleucel (axi-cel), a CAR T-cell therapy, yielded a complete response (CR) rate in 65% of patients, an improvement over the 32% CR rate when using standard chemotherapy. The ZUMA-7 trial highlights the potential of CAR T-cell therapy to treat traditionally resistant cancers, but work remains to be done to ide
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22

Sawaisorn, Piamsiri, Korakot Atjanasuppat, Usanarat Anurathapan, Somchai Chutipongtanate, and Suradej Hongeng. "Strategies to Improve Chimeric Antigen Receptor Therapies for Neuroblastoma." Vaccines 8, no. 4 (2020): 753. http://dx.doi.org/10.3390/vaccines8040753.

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Chimeric antigen receptors (CARs) are among the curative immunotherapeutic approaches that exploit the antigen specificity and cytotoxicity function of potent immune cells against cancers. Neuroblastomas, the most common extracranial pediatric solid tumors with diverse characteristics, could be a promising candidate for using CAR therapies. Several methods harness CAR-modified cells in neuroblastoma to increase therapeutic efficiency, although the assessment has been less successful. Regarding the improvement of CARs, various trials have been launched to overcome insufficient capacity. However
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23

Bosch, Berend Jan, Cornelis A. M. de Haan, and Peter J. M. Rottier. "Coronavirus Spike Glycoprotein, Extended at the Carboxy Terminus with Green Fluorescent Protein, Is Assembly Competent." Journal of Virology 78, no. 14 (2004): 7369–78. http://dx.doi.org/10.1128/jvi.78.14.7369-7378.2004.

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ABSTRACT Due to the limited ultrastructural information about the coronavirion, little is known about the interactions acting at the interface between nucleocapsid and viral envelope. Knowing that subtle mutations in the carboxy-terminal endodomain of the M protein are already lethal, we have now probed the equivalent domain of the spike (S) protein by extending it terminally with a foreign sequence of 27 kDa: the green fluorescent protein (GFP). When expressed individually in murine cells, the S-GFP chimeric protein induced the formation of fluorescent syncytia, indicating that it was synthes
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24

Immidisetti, Amanda, Sean Munier, and Nitesh Patel. "COVD-18. POTENTIAL TO HARNESS SARS-COV-2 NEUROTROPISM IN THE DELIVERY OF ONCOLYTIC VIROTHERAPY FOR THE TREATMENT OF HIGH-GRADE GLIOMA." Neuro-Oncology 22, Supplement_2 (2020): ii24—ii25. http://dx.doi.org/10.1093/neuonc/noaa215.101.

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Abstract BACKGROUND High-grade gliomas (HGG) pose therapeutic challenges stemming from blood brain barrier, infiltrative growth, suppressed immune function, and tumor heterogeneity. Oncolytic viruses (OVs) are gaining traction for addressing these challenges. There is evidence that the SARS-CoV-2 glycoprotein spike binds the ACE-2 receptor in nasal epithelium and reaches the brainstem and thalamus via axonal transport through the olfactory pathway, making it an attractive candidate for OV therapy. Prior studies on chimerization of pathogenic virus-derived glycoprotein spikes with non-pathogeni
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25

Jeong, Pil-Soo, Seung-Bin Yoon, Mun-Hyeong Lee, et al. "Embryo aggregation regulates in vitro stress conditions to promote developmental competence in pigs." PeerJ 7 (December 13, 2019): e8143. http://dx.doi.org/10.7717/peerj.8143.

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Embryo aggregation is a useful method to produce blastocysts with high developmental competence to generate more offspring in various mammals, but the underlying mechanism(s) regarding the beneficial effects are largely unknown. In this study, we investigated the effects of embryo aggregation using 4-cell stage embryos in in vitro developmental competence and the relationship of stress conditions in porcine early embryogenesis. We conducted aggregation using the well of the well system and confirmed that aggregation using two or three embryos was useful for obtaining blastocysts. Aggregated em
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26

MCCARTHY, SUSAN A., IRWIN J. GRIFFITH, PHILLIP GAMBEL, et al. "IMMUNOLOGICAL COMPETENCE AND HOST-SPECIFIC TOLERANCE OF ANTIBODY-FACILITATED BONE MARROW CHIMERAS." Transplantation 44, no. 1 (1987): 97–105. http://dx.doi.org/10.1097/00007890-198707000-00021.

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27

Yang, Yifang, Jingjun Lin, Anthony Harrington, Gabriel Cornilescu, Gee W. Lau, and Yftah Tal-Gan. "Designing cyclic competence-stimulating peptide (CSP) analogs with pan-group quorum-sensing inhibition activity in Streptococcus pneumoniae." Proceedings of the National Academy of Sciences 117, no. 3 (2020): 1689–99. http://dx.doi.org/10.1073/pnas.1915812117.

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Streptococcus pneumoniae is an opportunistic human pathogen that utilizes the competence regulon, a quorum-sensing circuitry, to acquire antibiotic resistance genes and initiate its attack on the human host. Interception of the competence regulon can therefore be utilized to study S. pneumoniae cell−cell communication and behavioral changes, as well as attenuate S. pneumoniae infectivity. Herein we report the design and synthesis of cyclic dominant negative competence-stimulating peptide (dnCSP) analogs capable of intercepting the competence regulon in both S. pneumoniae specificity groups wit
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28

KITO, Seiji, Yoshiko NOGUCHI, Yuki OHTA, et al. "Evaluation of Developmental Competence of Vitrified-warmed Early Cleavage Stage Embryos and their Application for Chimeric Mouse Production." Experimental Animals 52, no. 2 (2003): 179–83. http://dx.doi.org/10.1538/expanim.52.179.

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Lee, Joohyeong, Lian Cai, Mirae Kim, et al. "Developmental competence of chimeric porcine embryos through the aggregation of parthenogenetic embryos and somatic cell nuclear transfer embryos." Korean Journal of Veterinary Research 63, no. 1 (2023): e3. http://dx.doi.org/10.14405/kjvr.20230003.

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The efficiency of somatic cell nuclear transfer (NT) in pigs is low and requires enhancement. We identified the most efficient method for zona pellucida (ZP) removal and blastomere aggregation in pigs and investigated whether the aggregation of NT and parthenogenetic activation (PA) of blastomeres could reduce embryonic apoptosis and improve the quality of NT-derived embryos by investigating. Embryonic developmental competence after ZP removal using acid Tyrode's solution or protease (pronase E). The embryonic developmental potential of NT-derived blastomeres was also investigated using well-o
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30

Świerczek-Lasek, Barbara, Jacek Neska, Agata Kominek, et al. "Interleukin 4 Moderately Affects Competence of Pluripotent Stem Cells for Myogenic Conversion." International Journal of Molecular Sciences 20, no. 16 (2019): 3932. http://dx.doi.org/10.3390/ijms20163932.

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Pluripotent stem cells convert into skeletal muscle tissue during teratoma formation or chimeric animal development. Thus, they are characterized by naive myogenic potential. Numerous attempts have been made to develop protocols enabling efficient and safe conversion of pluripotent stem cells into functional myogenic cells in vitro. Despite significant progress in the field, generation of myogenic cells from pluripotent stem cells is still challenging—i.e., currently available methods require genetic modifications, animal-derived reagents, or are long lasting—and, therefore, should be further
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Loskutoff, N. M., and D. C. Kraemer. "Factors influencing the developmental competence of intraspecific murine chimeras produced by multiple embryo aggregation." Theriogenology 33, no. 1 (1990): 276. http://dx.doi.org/10.1016/0093-691x(90)90700-4.

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32

Gustems, Montse, Andreas Busche, Martin Messerle, Peter Ghazal, and Ana Angulo. "In Vivo Competence of Murine Cytomegalovirus under the Control of the Human Cytomegalovirus Major Immediate-Early Enhancer in the Establishment of Latency and Reactivation." Journal of Virology 82, no. 20 (2008): 10302–7. http://dx.doi.org/10.1128/jvi.01255-08.

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ABSTRACT The human cytomegalovirus (HCMV) major immediate-early enhancer has been postulated to play a pivotal role in the control of latency and reactivation. However, the absence of an animal model has obstructed a direct test of this hypothesis. Here we report on the establishment of an in vivo, experimentally tractable system for quantitatively investigating physiological functions of the HCMV enhancer. Using a neonate BALB/c mouse model, we show that a chimeric murine CMV under the control of the HCMV enhancer is competent in vivo, replicating in key organs of mice with acute CMV infectio
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33

Lee, Myeong S., Brian A. Dougherty, Anne C. Madeo, and Donald A. Morrison. "Construction and Analysis of a Library for Random Insertional Mutagenesis in Streptococcus pneumoniae: Use for Recovery of Mutants Defective in Genetic Transformation and for Identification of Essential Genes." Applied and Environmental Microbiology 65, no. 5 (1999): 1883–90. http://dx.doi.org/10.1128/aem.65.5.1883-1890.1999.

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ABSTRACT To explore the use of insertion-duplication mutagenesis (IDM) as a random gene disruption mutagenesis tool for genomic analysis ofStreptococcus pneumoniae, a large mutagenic library of chimeric plasmids with 300-bp inserts was constructed. The library was large enough to produce 60,000 independent plasmid clones inEscherichia coli. Sequencing of a random sample of 84 of these clones showed that 85% of the plasmids had inserts which were scattered widely over the genome; 80% of these plasmids had 240- to 360-bp inserts, and 60% of the inserts targeted internal regions of apparent open
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34

Peters, Okimi, and W. Allan King. "The detection of female cell activity in male sex chromosome chimeric Rideau Arcott sheep, using the Xist gene product as a marker." SURG Journal 1, no. 2 (2008): 20–25. http://dx.doi.org/10.21083/surg.v1i2.414.

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The detection of the SRY (Sex-determining region on the Y chromosome) gene is a popular method used for the identification of freemartins (XX/XY female chimeras). This method relies on the fact that the SRY gene is a Y chromosome specific gene and is thus normally only present in males therefore detecting its presence in a female indicates the presence of male cells (XY cells) within the female. This concept can be extrapolated to the male counterparts of freemartins with regards to the Xist gene. This gene is normally only widely expressed in females and can be used as a marker for identifyin
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35

Carstea, Ana Claudia. "Germline competence of mouse ES and iPS cell lines: Chimera technologies and genetic background." World Journal of Stem Cells 1, no. 1 (2009): 22. http://dx.doi.org/10.4252/wjsc.v1.i1.22.

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36

Klco, Jeffery M., Saurabh Sen, Jakob L. Hansen, et al. "Complement factor 5a receptor chimeras reveal the importance of lipid-facing residues in transport competence." FEBS Journal 276, no. 10 (2009): 2786–800. http://dx.doi.org/10.1111/j.1742-4658.2009.07002.x.

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37

Rüedi, E., M. Sykes, S. T. Ildstad, et al. "Antiviral T cell competence and restriction specificity of mixed allogeneic (P1 + P2 → P1) irradiation chimeras." Cellular Immunology 121, no. 1 (1989): 185–95. http://dx.doi.org/10.1016/0008-8749(89)90016-6.

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38

Peranteau, William H., Masayuki Endo, Obinna O. Adibe, and Alan W. Flake. "Evidence for an immune barrier after in utero hematopoietic-cell transplantation." Blood 109, no. 3 (2006): 1331–33. http://dx.doi.org/10.1182/blood-2006-04-018606.

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Abstract The competence of the immune system of the developing fetus to act as a barrier to in utero hematopoietic-cell transplantation (IUHCT) has been a source of debate. Until now, comparisons of allogeneic and congenic engraftment have been inconclusive due to methodologic limitations resulting in minimal and inefficient engraftment. In this study, E14 fetal mice received transplants of either allogeneic or congenic bone marrow using a new intravascular technique that allows definitive administration of much higher doses of donor cells. Our results demonstrate that 100% of surviving recipi
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39

Peranteau, William H., Masayuki Endo, Obinna O. Adibe, and Alan W. Flake. "Evidence for an Adaptive Immune Barrier after in Utero Hematopoietic Cell Transplantation." Blood 108, no. 11 (2006): 3179. http://dx.doi.org/10.1182/blood.v108.11.3179.3179.

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Abstract In utero hematopoietic cell transplantation (IUHCT) is a nonmyeloablative approach that takes advantage of normal immunologic development to achieve donor specific tolerance. Despite the many potential advantages of the fetal recipient, IUHCT across MHC barriers has been limited by low levels of engraftment and the inability to consistently achieve allochimerism. Although the immature immune system of the developing fetus has long been appreciated as a principal advantage of IUHCT, the competence of the fetal immune system to act as a barrier to IUHCT has been a source of debate. Unti
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40

Krüger, Nadine, Christian Sauder, Sarah Hüttl, et al. "Entry, Replication, Immune Evasion, and Neurotoxicity of Synthetically Engineered Bat-Borne Mumps Virus." Cell Reports 25, no. 2 (2018): 312–20. https://doi.org/10.5281/zenodo.13452751.

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(Uploaded by Plazi for the Bat Literature Project) Bats harbor a plethora of viruses with an unknown zoonotic potential. In-depth functional characterization of such viruses is often hampered by a lack of virus isolates. The genome of a virus closely related to human mumps viruses (hMuV) was detected in African fruit bats, batMuV. Efforts to characterize batMuV were based on directed expression of the batMuV glycoproteins or use of recombinant chimeric hMuVs harboring batMuV glycoprotein. Although these studies provided initial insights into the functionality of batMuV glycoproteins, the host
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41

Krüger, Nadine, Christian Sauder, Sarah Hüttl, et al. "Entry, Replication, Immune Evasion, and Neurotoxicity of Synthetically Engineered Bat-Borne Mumps Virus." Cell Reports 25, no. 2 (2018): 312–20. https://doi.org/10.5281/zenodo.13452751.

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(Uploaded by Plazi for the Bat Literature Project) Bats harbor a plethora of viruses with an unknown zoonotic potential. In-depth functional characterization of such viruses is often hampered by a lack of virus isolates. The genome of a virus closely related to human mumps viruses (hMuV) was detected in African fruit bats, batMuV. Efforts to characterize batMuV were based on directed expression of the batMuV glycoproteins or use of recombinant chimeric hMuVs harboring batMuV glycoprotein. Although these studies provided initial insights into the functionality of batMuV glycoproteins, the host
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42

Krüger, Nadine, Christian Sauder, Sarah Hüttl, et al. "Entry, Replication, Immune Evasion, and Neurotoxicity of Synthetically Engineered Bat-Borne Mumps Virus." Cell Reports 25, no. 2 (2018): 312–20. https://doi.org/10.5281/zenodo.13452751.

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(Uploaded by Plazi for the Bat Literature Project) Bats harbor a plethora of viruses with an unknown zoonotic potential. In-depth functional characterization of such viruses is often hampered by a lack of virus isolates. The genome of a virus closely related to human mumps viruses (hMuV) was detected in African fruit bats, batMuV. Efforts to characterize batMuV were based on directed expression of the batMuV glycoproteins or use of recombinant chimeric hMuVs harboring batMuV glycoprotein. Although these studies provided initial insights into the functionality of batMuV glycoproteins, the host
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43

Krüger, Nadine, Christian Sauder, Sarah Hüttl, et al. "Entry, Replication, Immune Evasion, and Neurotoxicity of Synthetically Engineered Bat-Borne Mumps Virus." Cell Reports 25, no. 2 (2018): 312–20. https://doi.org/10.5281/zenodo.13452751.

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(Uploaded by Plazi for the Bat Literature Project) Bats harbor a plethora of viruses with an unknown zoonotic potential. In-depth functional characterization of such viruses is often hampered by a lack of virus isolates. The genome of a virus closely related to human mumps viruses (hMuV) was detected in African fruit bats, batMuV. Efforts to characterize batMuV were based on directed expression of the batMuV glycoproteins or use of recombinant chimeric hMuVs harboring batMuV glycoprotein. Although these studies provided initial insights into the functionality of batMuV glycoproteins, the host
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44

Krüger, Nadine, Christian Sauder, Sarah Hüttl, et al. "Entry, Replication, Immune Evasion, and Neurotoxicity of Synthetically Engineered Bat-Borne Mumps Virus." Cell Reports 25, no. 2 (2018): 312–20. https://doi.org/10.5281/zenodo.13452751.

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Resumen
(Uploaded by Plazi for the Bat Literature Project) Bats harbor a plethora of viruses with an unknown zoonotic potential. In-depth functional characterization of such viruses is often hampered by a lack of virus isolates. The genome of a virus closely related to human mumps viruses (hMuV) was detected in African fruit bats, batMuV. Efforts to characterize batMuV were based on directed expression of the batMuV glycoproteins or use of recombinant chimeric hMuVs harboring batMuV glycoprotein. Although these studies provided initial insights into the functionality of batMuV glycoproteins, the host
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45

Liu, J., M. P. Ashton, H. Sumer, T. C. Brodnicki, M. K. O'Bryan, and P. J. Verma. "221 GENERATION OF GERM-LINE COMPETENT EMBRYONIC STEM CELLS FROM NON-OBESE DIABETIC (NOD) MICE USING A SINGLE INHIBITOR." Reproduction, Fertility and Development 24, no. 1 (2012): 222. http://dx.doi.org/10.1071/rdv24n1ab221.

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The use of inbred mouse strains, where selective mating and tissue availability can be used to study the role of specific genes during disease pathogenesis is a synergistic approach to human type 1 diabetes (T1D) studies, which are hindered by genetic heterogeneity and inability to obtain relevant tissue biopsies. An excellent model for T1D is the non-obese diabetic (NOD) mouse strain, which spontaneously develops autoimmunity with lymphocytic infiltration of pancreatic islets. Autoimmune disease in NOD mice is multigenic and more than 20 susceptibility loci have been identified. Thus, genetic
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46

Chen, Song-Lin, Zhen-Xia Sha, Han-Qing Ye, et al. "Pluripotency and Chimera Competence of an Embryonic Stem Cell Line from the Sea Perch (Lateolabrax japonicus)." Marine Biotechnology 9, no. 1 (2006): 82–91. http://dx.doi.org/10.1007/s10126-006-6050-1.

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47

Diego-Mantecón, José Manuel, Elena Haro, Teresa F. Blanco, and Avenilde Romo-Vázquez. "The chimera of the competency-based approach to teaching mathematics: a study of carpentry purchases for home projects." Educational Studies in Mathematics 107, no. 2 (2021): 339–57. http://dx.doi.org/10.1007/s10649-021-10032-5.

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48

Labosky, P. A., D. P. Barlow, and B. L. Hogan. "Mouse embryonic germ (EG) cell lines: transmission through the germline and differences in the methylation imprint of insulin-like growth factor 2 receptor (Igf2r) gene compared with embryonic stem (ES) cell lines." Development 120, no. 11 (1994): 3197–204. http://dx.doi.org/10.1242/dev.120.11.3197.

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Primordial germ cells of the mouse cultured on feeder layers with leukemia inhibitory factor, Steel factor and basic fibroblast growth factor give rise to cells that resemble undifferentiated blastocyst-derived embryonic stem cells. These primordial germ cell-derived embryonic germ cells can be induced to differentiate extensively in culture, form teratocarcinomas when injected into nude mice and contribute to chimeras when injected into host blastocysts. Here, we report the derivation of multiple embryonic germ cell lines from 8.5 days post coitum embryos of C57BL/6 inbred mice. Four independ
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49

Boyd, Nicholas, Kellie Cartledge, Huimin Cao, et al. "‘Off-the-Shelf’ Immunotherapy: Manufacture of CD8+ T Cells Derived from Hematopoietic Stem Cells." Cells 10, no. 10 (2021): 2631. http://dx.doi.org/10.3390/cells10102631.

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Cellular immunotherapy is revolutionizing cancer treatment. However, autologous transplants are complex, costly, and limited by the number and quality of T cells that can be isolated from and expanded for re-infusion into each patient. This paper demonstrates a stromal support cell-free in vitro method for the differentiation of T cells from umbilical cord blood hematopoietic stem cells (HSCs). For each single HSC cell input, approximately 5 × 104 T cells were created with an initial five days of HSC expansion and subsequent T cell differentiation over 49 days. When the induced in vitro differ
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50

Sato, Hideaki, Ayumi Wakayama, Kyoko Ito, Ikuo Kashiwakura, and Koichi Ito. "Functional Adaptive Immune Responses in Hematopoietic Chimeric Mice After Umbilical Cord Blood Cell Transplantation." Blood 120, no. 21 (2012): 2995. http://dx.doi.org/10.1182/blood.v120.21.2995.2995.

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Abstract Abstract 2995 Introduction: An increasing number of clinical trials have demonstrated the usefulness of cord blood as a source of hematopoietic stem cells for reconstitution of the hematopoietic system. Nevertheless, due to a lack of convenient animal models, information about the immunological competence of umbilical cord blood cell (UCBC)-derived lymphocytes has been relatively limited. Recently, we have established a murine model of UCBC transplantation, which reconstitutes the hematopoietic system of immunodeficient mice, and studied the correct immunological functions of UCBC-der
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