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Tesis sobre el tema "Confocal fluorescence microscopy"

Autor: Grafiati

Publicado: 4 de junio de 2021

Última modificación: 24 de enero de 2023

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1

Eigenbrot, Ilya Vladimirovich. "A time-resolved confocal fluorescence microscope." Thesis, Imperial College London, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342331.

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Alawadhi, Fahimah. "Statistical image analysis and confocal microscopy." Thesis, University of Bath, 2001. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.341639.

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3

Jiang, Shihong. "Non-scanning fluorescence confocal microscopy using laser speckle illumination." Thesis, University of Nottingham, 2005. http://eprints.nottingham.ac.uk/10139/.

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Confocal scanning microscopy (CSM) is a much used and advantageous form of microscopy. Although CSM is superior to conventional microscopy in many respects, a major disadvantage is the complexity of the scanning process and the sometimes long time to perform the scan. In this thesis a novel non-scanning fluorescence confocal microscopy is investigated. The method uses a random time-varying speckle pattern to illuminate the specimen, recording a large number of independent full-field frames without the need for a scanning system. The recorded frames are then processed in a suitable way to give

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4

Wang, Xiao. "Confocal angle resolved linear dichroism microscopy for structural fluorescence imaging." Ecole centrale de Marseille, 2013. http://tel.archives-ouvertes.fr/docs/00/87/10/10/PDF/Wang-Thesis.pdf.

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La microscopie de fluorescence a récemment été complétée par une technique appelée dichroïsme linéaire résolu angulairement, basé sur le fait que l'absorption de la lumière est un processus sensible à l'orientation moléculaire. En analysant la réponse d'émission de fluorescence en fonction de l'orientation de la polarisation de la lumière excitatrice, cette technique permet de remonter à l'information d'orientation sur un ensemble de molécules fluorescentes, plus précisément son angle d'orientation moyenne et l'amplitude de ses fluctuations angulaires autour de cette moyenne. Dans cette thèse,

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5

Gösch, Michael. "Microfluidic analysis and parallel confocal detection of single molecules /." Stockholm, 2003. http://diss.kib.ki.se/2003/91-7349-663-4/.

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6

Risi, Matthew D. "Advances In Combined Endoscopic Fluorescence Confocal Microscopy And Optical Coherence Tomography." Diss., The University of Arizona, 2014. http://hdl.handle.net/10150/332772.

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Confocal microendoscopy provides real-time high resolution cellular level images via a minimally invasive procedure. Results from an ongoing clinical study to detect ovarian cancer with a novel confocal fluorescent microendoscope are presented. As an imaging modality, confocal fluorescence microendoscopy typically requires exogenous fluorophores, has a relatively limited penetration depth (100μm), and often employs specialized aperture configurations to achieve real-time imaging in vivo. Two primary research directions designed to overcome these limitations and improve diagnostic capability a

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7

Slimani, Amel. "Photonic approach for the study of dental hard tissues and carious lesion detection." Thesis, Montpellier, 2017. http://www.theses.fr/2017MONTT125.

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Les propriétés photoniques des tissus durs dentaires nous ont permis d’étudier l’email et la dentine a un niveau moléculaire (in vitro) en utilisant des techniques de microscopie optique non linéaires. La microscopie confocale Raman est technique d’imagine de haute résolution permettant d’analyse d’échantillon sans préparation spécifique ni marquage. Cette méthode nous a permis de reconstituer une cartographie de la réticulation du collagène et de la cristallinité au niveau de la jonction émail-dentine et cela avec une résolution spatiale non atteinte jusque-là. Cette analyse c

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8

Tsutae, Fernando Massayuki. "Espectroscopia de correlação de fluorescência aplicada em estudos de sistemas moleculares, biológicos e celulares." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/76/76132/tde-14102016-101124/.

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A espectroscopia de correlação de fluorescência (FCS) é uma das diferentes técnicas de análise por imagens de alta resolução espacial e temporal de biomoléculas em concentrações extremamente baixas. Ela se tornou uma técnica extremamente poderosa e sensível em áreas como bioquímica e biofísica. Como uma técnica bem estabelecida, ela é utilizada para medir concentrações locais de biomoléculas, através da marcação com moléculas fluorescentes. Coeficientes de difusão e constantes cinéticas também podem ser medidos através de FCS assim como detecção de molécula única. Ela também pode dar informaçã

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9

Kakade, Rohan. "Improved resolution and signal-to-noise ratio performance of a confocal fluorescence microscope." Thesis, University of Nottingham, 2016. http://eprints.nottingham.ac.uk/33699/.

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A culmination of theory, techniques and devices stemming from a wide variety of sources and disciplines, optical microscopy presents vast possibilities for visualisation of small structures. One of the most fundamental yet significant optical microscopy techniques is Confocal Fluorescence Microscopy (CFM). CFM is studied here by analysing its performance with respect to the two most important metrics - Signal-to-noise ratio and 3D optical resolution. Several authors have commented on the inherent inefficiency of imaging systems such as CFM to utilise the available light when providing resoluti

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10

Ferro, Daniela Peixoto 1981. "Aplicação da biofotônica para o estudo de cicatrizes." [s.n.], 2015. http://repositorio.unicamp.br/jspui/handle/REPOSIP/312786.

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Orientador: Konradin Metze<br>Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas<br>Made available in DSpace on 2018-08-26T20:44:11Z (GMT). No. of bitstreams: 1 Ferro_DanielaPeixoto_D.pdf: 2964245 bytes, checksum: 202d309cf65f632d47c7811d4958535d (MD5) Previous issue date: 2015<br>Resumo: A aplicação integrada de técnicas modernas, como a Geração do Segundo Harmônico (SHG) e os tempos de vida da fluorescência (FLIM), com análise de imagens matemáticas nos permitem visualizar detalhes não vistos por microscopia de luz convencional. O objetivo deste estudo foi

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11

Maggiano, Corey. "CONFOCAL LASER SCANNING MICROSCOPY AS A TOOL FOR THE INVESTIGATION OF TETRACYCLINE FLUORESCENCE IN ARCHAEOLOGICALHUMAN BONE." Master's thesis, University of Central Florida, 2005. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/2752.

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Fluorochromes such as tetracycline have been used to label bone for histomorphometric analysis, measuring bone formation, growth, maintenance, and pathology. More recently, similar fluorescence has been observed in ancient human bone. Attributed to tetracycline (TC) exposure, this phenomenon could affect various aspects of health during life and/or preservation of remains postmortem. Standard epifluorescence microscopy is the most common tool employed in the analysis of these labels. Though valuable, this technique is limited by its inability to penetrate bone three-dimensionally and its inclu

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12

Hu, Yan. "Quantitative confocal imaging of nanoporous silica." Diss., University of Iowa, 2016. https://ir.uiowa.edu/etd/3106.

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Nanoporous materials have been widely used in the fields of biological and chemical sensing, chemical separation, heterogeneous catalysis and biomedicine due to their merits of high surface area-to-volume ratio, chemical and thermal stabilities, and flexible surface modification. However, as the nature of nanoporous materials, they are inherently heterogeneous in the micro- and nanoenvironments. The environmental heterogeneity plays a decisive role in determining the performance of various applications of nanoporous materials. In order to provide an in-depth understanding of the nanoporous mat

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13

Hermann, Martin, Oliver Nussbaumer, Ralf Knöfler, Paul Hengster, Walter Nussbaumer, and Werner Streif. "Real-Time Live Confocal Fluorescence Microscopy as a New Tool for Assessing Platelet Vitality." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-136619.

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Background: Assessment of platelet vitality is important for patients presenting with inherited or acquired disorders of platelet function and for quality assessment of platelet concentrates. Methods: Herein we combined live stains with intra-vital confocal fluorescence microscopy in order to obtain an imaging method that allows fast and accurate assessment of platelet vitality. Three fluorescent dyes, FITC-coupled wheat germ agglutinin (WGA), tetramethylrhodamine methyl ester perchlorate (TMRM) and acetoxymethylester (Rhod-2), were used to assess platelet morphology, mitochondrial activity an

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14

Hermann, Martin, Oliver Nussbaumer, Ralf Knöfler, Paul Hengster, Walter Nussbaumer, and Werner Streif. "Real-Time Live Confocal Fluorescence Microscopy as a New Tool for Assessing Platelet Vitality." Karger, 2010. https://tud.qucosa.de/id/qucosa%3A26667.

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Background: Assessment of platelet vitality is important for patients presenting with inherited or acquired disorders of platelet function and for quality assessment of platelet concentrates. Methods: Herein we combined live stains with intra-vital confocal fluorescence microscopy in order to obtain an imaging method that allows fast and accurate assessment of platelet vitality. Three fluorescent dyes, FITC-coupled wheat germ agglutinin (WGA), tetramethylrhodamine methyl ester perchlorate (TMRM) and acetoxymethylester (Rhod-2), were used to assess platelet morphology, mitochondrial activity an

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15

Clarke, Oliver J. "Isothiocyanato porphyrins for bioconjugation : synthesis and applications in targeted photochemotherapy and fluorescence imaging." Thesis, University of Essex, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.327076.

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16

Kaldaras, Leonora. "Single Molecule Studies of Enzymes Horseradish Peroxidase and Alkaline Phosphatase Using Total Internal Reflection Fluorescence Microscopy and Confocal Microscopy." Bowling Green State University / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1374686174.

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17

Dubaj, Vladimir, and n/a. "Novel optical fluorescence imaging probe for the investigation of biological function at the microscopic level." Swinburne University of Technology, 2005. http://adt.lib.swin.edu.au./public/adt-VSWT20060905.084615.

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Existing optic fibre-bundle based imaging probes have been successfully used to image biological signals from tissue in direct contact with the probe tip (Hirano et al. 1996). These fibre-bundle probe systems employed conventional fluorescence microscopy and thus lacked spatial filtering or a scanned light source, two features used by laser scanning confocal microscopes (LSCMs) to improve signal quality. Improving the methods of imaging tissue in its natural state, deep in-vivo and at cellular resolution is an ever-present goal in biological research. Within this study, a novel (580 μm diamete

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18

Araújo, Cíntia Tereza Pimenta de 1968. "Use of fluorescence in the performance evaluation of adhesive systems = Uso da fluorescência na avaliação do comportamento de sistemas adesivos." [s.n.], 2013. http://repositorio.unicamp.br/jspui/handle/REPOSIP/289658.

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Orientador: Luis Alexandre Maffei Sartinni Paulillo<br>Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba<br>Made available in DSpace on 2018-08-21T22:09:59Z (GMT). No. of bitstreams: 1 Araujo_CintiaTerezaPimentade_D.pdf: 9367664 bytes, checksum: 6c7161dae0f158a143f829e530249b20 (MD5) Previous issue date: 2013<br>Resumo: A microscopia confocal de varredura a laser (MCVL) é um recurso de visualização microscópica que permite a análise de materiais ou estruturas com requisitos mínimos de preparação de amostras de modo não destrutivo. Assim, os objetivo

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19

Beaufort, Sandra. "Développement d'outils et de méthodologies pour l'étude de l'organisation et de la localisation in vivo de micro-organismes dans des structures biologiques complexes." Thesis, Toulouse, INSA, 2010. http://www.theses.fr/2010ISAT0037/document.

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Ce projet concerne l’analyse de l’organisation de populations microbiennes au sein de structures complexescomme des dépôts ou des biofilms.Si différentes méthodes sont couramment utilisées pour étudier la structure globale ou l’organisation localed’agrégats microbiens, peu d’entre elles, permettent de réaliser simultanément ces deux analyses et nécessitentsouvent des étapes de préparation qui pénalisent un approche in-vivo et en dynamique.La stratégie proposée repose sur la mise en oeuvre de micro-organismes modèles autofluorescents (levures etbactéries) qui peuvent sans aucun traitement être

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20

Doroshenko, Mikheil [Verfasser]. "Diffusion in heterogeneous systems studied by laser scanning confocal microscopy and fluorescence correlation spectroscopy / Mikheil Doroshenko." Mainz : Universitätsbibliothek Mainz, 2014. http://d-nb.info/104870758X/34.

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21

Sirdeshmukh, Ranjani. "Biological functionalization of single-wall carbon nanotubes." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file 0.97Mb, 59 p, 2005. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pqdiss&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft_dat=xri:pqdiss:1428206.

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22

Vaillancourt, Benoit. "Novel biophysical appliations [sic] of STICS." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=111550.

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The object of this thesis is to present two novel applications of Spatiotemporal Image Correlation Spectroscopy (STICS) to biological systems. STICS is a technique which uses the correlations in pixel intensity fluctuations of an image time series, captured under fluorescence microscopy, to measure the speed and direction of a flowing population of fluorescently labeled molecules. The method was first applied to measure the dynamics of transport vesicles inside growing pollen tubes of lily flowers. The measured vector maps allowed to confirm the presence of actin filaments along the periphery

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23

Grunwald, Matthias. "Molekulare Orientierung als Kontrastmechanismus in der Fluoreszenzmikroskopie und konfokale Multidetektor-Scanning-Mikroskopie." Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2015. http://hdl.handle.net/11858/00-1735-0000-0028-87C4-9.

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Die vorliegende Arbeit befasst sich mit zwei neuen methodischen Ansätzen auf dem Gebiet der Fluoreszenzmikroskopie. Im ersten Teil der Arbeit wir eine Methode vorgestellt, mit der die Winkelselektivität der Fluoreszenzanregung verbessert werden kann. Die ExPAN (excitation polarization angle narrowing) genannte Technik nutzt stimulierte Emission, um den Effekt der Photoselektion zu vergrößern. ExPAN lässt sich potentiell für verschiedene Methoden einsetzen, in denen fluoreszenzmarkierte Proben untersucht werden und ist insbesondere im Kontext von Fluoreszenzanisotropie-Messungen oder der Besti

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24

Gao, Yongxiang. "Direct observation of correlated motions in colloidal gels and glasses." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=115677.

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Dynamical heterogeneity (DH) has been observed in many systems approaching the glass or jamming transition. Whether DH has a structural origin is under heated debate. To provide a deeper understanding, in this thesis I investigate the microscopic dynamics in weakly attractive colloidal systems by confocal fluorescence microscopy. The van Hove density-density correlation function is applied to our systems. Separable fast and slow populations emerge in the self part (svH), while the distinct part shows a strong signature of DH close to the gel transition. At intermediate time, svH shows a purely

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25

Falvo, Maurício. "Método de mapeamento espaço-espectral em imagens multi-espectrais e sua aplicação em tecidos vegetais." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/76/76132/tde-15012016-164547/.

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Imagens multiespectrais são utilizadas em diferentes aplicações, que vão desde sensoriamento remoto a processos médicos. No caso de imagens multiespectrais oriundas de microscopia confocal de varredura à laser (Confocal Laser Scanning Microscopy-CLSM), a extração da informação se inicia pela conversão das assinaturas espectrais, em uma imagem RGB. Esta imagem é a referência para a seleção da região de interesse, da qual se obtém a assinatura espectral média, originada do arquivo multiespectral (LSM). Mesmo utilizando um padrão muito bem estabelecido de conversão, alguns pontos devem ser consid

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26

Zdankowski, Piotr. "Adaptive optics stimulated emission depletion microscope for thick sample imaging." Thesis, University of Dundee, 2018. https://discovery.dundee.ac.uk/en/studentTheses/90e27151-f51c-4c12-b9dd-2bc78beb2321.

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Over the past few decades, fluorescence microscopy has proven to become the most widely used imaging technique in the field of life sciences. Unfortunately, all classical optical microscopy techniques have one thing in common: their resolution is limited by the diffraction. Thankfully, due to the very strong interest, development of fluorescent microscopy techniques is very intense, with novel solutions surfacing repeatedly. The major breakthrough came with the appearance of super-resolution microscopy techniques, enabling imaging well below the diffraction barrier and opening the new era of n

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27

Makhlouf, Houssine. "Integrated Multi-Spectral Fluorescence Confocal Microendoscope and Spectral-Domain Optical Coherence Tomography Imaging System for Tissue Screening." Diss., The University of Arizona, 2011. http://hdl.handle.net/10150/202761.

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A multi-modality imaging system intended for clinical utilization has been developed. It is constructed around an existing fiber-bundle-based fluorescence confocal microendoscope. Additional imaging modalities have been implemented to expand the capabilities of the system and improve the accuracy of disease diagnosis. A multi-spectral mode of operation is one such modality. It acquires fluorescence images of a biological sample across a spectral range of sensitivity and explores the collected image data at any specified wavelength within that spectral range. Cellular structures can be differen

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28

Junior, Odair Bim. "Estratégias para adição de rodamina B em sistemas adesivos." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/25/25148/tde-14082013-102351/.

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A rodamina B e outros marcadores fluorescentes vêm sendo empregados na avaliação morfológica da interface de adesão com o auxílio de microscopia confocal de varredura a laser. Acrescentando-se rodamina B a sistemas adesivos, é possível observar as características de espessura da camada de adesivo, micromorfologia da camada híbrida, extensão e quantidade de tags, bem como diagnosticar defeitos ou alterações na interface de adesão. A literatura revela, entretanto, uma falta de precedentes sobre as quantidades de marcadores nos componentes resinosos. Além disso, ainda não se avaliou o efeito da c

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29

King, Mathias. "Development of new bioorthogonal ligation reactions." Thesis, Strasbourg, 2013. http://www.theses.fr/2013STRAF021.

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Le principal objectif de cette thèse a consisté au développement d’une méthode de screenning pour la découverte de nouvelles réactions de ligations bioorthogonales ainsi que son application sur une bibliothèque développée pour cette étude. Par conséquent, un système de screening a été conçu en trois étapes consistant au départ en une analyse HPLC, puis une évaluation basée sur la fluorescence de haute résolution et finalement un test de microscopie confocal in cellulo. Puis, nous avons standardisé toutes les analyses avec les réactions CuAAC et SpAAC. En outre, nous avons synthétisés 18 réacti

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30

Jones, Christopher Wynne. "Laser scanning confocal arthroscopy in orthopaedics : examination of chondrial and connective tissues, quantification of chondrocyte morphology, investigation of matirx-induced autologous chondrocyte implantation and characterisation of osteoarthritis." University of Western Australia. School of Mechanical Engineering, 2007. http://theses.library.uwa.edu.au/adt-WU2008.0061.

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[Truncated abstract] Articular cartilage (AC) covers the surface of synovial joints providing a nearly frictionless bearing surface and distributing mechanical load. Joint trauma can damage the articular surface causing pain, loss of mobility and deformation. Currently there is no uniform treatment protocol for managing focal cartilage defects, with most treatment options targeted towards symptomatic relief but not limiting the progression into osteoarthritis (OA). Autologous chondrocyte implantation (ACI) and more recently matrix-induced autologous chondrocyte implantation (MACI), have emerge

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31

Reitan, Nina Kristine. "Methods for studying critical barriers to the delivery of nanomedicine : The potential of fluorescence correlation spectroscopy, confocal microscopy and MRI." Doctoral thesis, Norges teknisk-naturvitenskapelige universitet, Institutt for fysikk, 2009. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-5760.

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32

Schumacher, William Charles. "Development of Novel Fluorescence-Based Methods for Detection of Bacillus Anthracis Spores." The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1222046829.

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33

Wenzel, Margot. "Synthèse de complexes organobimétalliques à activité biologique." Thesis, Dijon, 2013. http://www.theses.fr/2013DIJOS089.

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Un axe de recherche particulièrement développé en chimie organométallique moderneconcerne la recherche d’agents chimiothérapeutiques alternatifs au cisplatine dans lestraitements contre le cancer. Dans ce domaine, plusieurs stratégies ont été développées depuisplusieurs dizaines d’années, parmi lesquelles nous pouvons citer la multinucléarité, consistanten la création d’édifices polymétalliques. L’objectif de la première partie de ce manuscrits’inscrit dans cette thématique, par la conception, l’obtention et l’étude des propriétésbiologiques de complexes hétérobimétalliques "early-late" (Ti-Ru

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34

Romano, Renan Arnon. "Aumento da eficácia da inativação fotodinâmica pela incorporação de fotossensibilizador induzida por luz em Candida Albicans." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/76/76132/tde-21102016-103320/.

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Devido à grande preocupação com a geração de novas tecnologias mais ágeis, eficientes e de baixo custo, as reações fotodinâmicas (RF) têm ganhado destaque. Estas envolvem a ativação de um fármaco fotossensível (fotossensibilizador (FS)), pela iluminação em uma faixa específica de comprimentos de onda, gerando assim espécies reativas altamente citotóxicas levando as células à morte. As RF são divididas em duas categorias: terapia fotodinâmica (TFD) e inativação fotodinâmica (IFD). A primeira é utilizada para tratamentos oncológicos, dermatológicos, entre outros. A segunda é utilizada para desco

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35

Sadler, Emma Elizabeth. "Single-molecule fluorescence studies of KirBac1.1." Thesis, University of Oxford, 2015. http://ora.ox.ac.uk/objects/uuid:214fcd74-7384-4ade-ac17-7cac5c44a05c.

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Inwardly rectifying potassium (Kir) channels are essential for controlling the excitability of eukaryotic cells, forming a key part of the inter-cellular signalling system in multi-cellular organisms. However, as prokaryotic (KirBac) channels are less technically challenging to study in vitro and have been shown to be directly homologous to eukaryotic channels, they are often studied in lieu of their mammalian counterparts. A vital feature of Kir and KirBac channels is their mechanism for opening and closing, or their gating: this study predominantly features observations of open and/or closed

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36

Ying, Jia. "Structural Change and Its Assessment by Fluorescence Spectroscopy in Functional Polymers." 京都大学 (Kyoto University), 2014. http://hdl.handle.net/2433/192187.

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37

Garcia, Pérez José Antonio. "Microscopies Optiques et Spectroscopies de Matériaux Épais : Mesures et Simulations Appliquées à des Photosensibilisateurs de l'Oxygène Singulet en Matrice de Silice." Thesis, Pau, 2013. http://www.theses.fr/2013PAUU3015/document.

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Ce travail présente une étude, par microscopie optique et de fluorescence, de matériaux hybrides, basés sur des monolithes de silice contenant des dérivés du cyano-anthracène : 9,10-dicyano-anthracène (DCA) ou 9,14-dicyano-benzo(b)triphénylène (DBTP), qui sont des photo-sensibilisateurs de l'oxygène singulet. Alors que ces matériaux sont bien caractérisés du point de vue de la photo-oxydation des sulfures sous des conditions hétérogènes par des études macroscopiques, certaines propriétés concernant l'association du photosensibilisateur avec l'absorbant peuvent être masquées, dominées ou encore

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38

Barsan, Cristina Ioana. "Caractérisation du chromoplaste de tomate par approche protéomique." Thesis, Toulouse, INPT, 2010. http://www.theses.fr/2010INPT0046/document.

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La maturation des fruits est un processus complexe, principalement régulé par l'hormone végétal éthylène, qui entraîne d'importants changements métaboliques et physiologiques, ayant pour résultat la dispersion des graines. Le changement le plus visible qui se produit pendant la maturation des fruits est le changement de couleur. L'organite responsable de ce phénomène est le chromoplaste, lieu d’accumulation des caroténoïdes. Toutefois, ce n'est pas son unique rôle. Il a été montré qu’il est aussi impliqué dans la biosynthèse des lipides, de l’amidon, des vitamines et des arômes. Parce que la p

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Wang, Huan. "Multiphotonic study of a new NADPH-derivative compound targeting NO-synthase." Phd thesis, École normale supérieure de Cachan - ENS Cachan, 2013. http://tel.archives-ouvertes.fr/tel-00958076.

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Dans cette étude, nous avons développé un composé dérivé du NADPH, nommé Nanoshutter (NS). NS a été conçu pour inhiber l'activité catalytique de la NOS, c'est à dire la synthèse de NO, en occupant la place du NADPH dans le domaine réductase du NOS. La voie de synthèse de NO chez les mammifère correspond à l'oxydation de la L-arginine catalysée par la NOS, qui se produit dans son domaine oxygénase. Basée sur des données de modélisation moléculaire, la structure de NS est composée deux sous-unités: (i) le motif nucléotidique de reconnaissance du NADPH a été retenu, permettant au composé NS un ci

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Larsson, Mina. "Application of Raman and Fluorescence Spectroscopy to Single Chromatographic Beads." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5741.

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Chimenez, Tiago Andrade. "Estudos de sistemas poliméricos naturais e sintéticos utilizando técnicas avançadas de microscopia." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/75/75134/tde-26072016-152852/.

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O bagaço de cana-de-açúcar é um abundante coproduto obtido a partir da produção convencional de etanol. No entanto, o bagaço vem se mostrando como uma importante fonte para a produção de etanol de segunda geração. No primeiro capítulo da tese é apresentado um estudo referente à distribuição espacial dos compostos na matriz de bagaço de cana-de-açúcar. A investigação foi realizada utilizando microscopia de fluorescência confocal e espectroscopia por excitação com um e dois fótons. Imagens de autofluorescência em combinação com as medidas de fluorescência e tempos de vida forneceram uma gama de

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42

Fujita, Alessandra Keiko Lima. "Avaliação do efeito fotodinâmico a partir da associação dos precursores da PPIX (ALA e MAL) em epitélio suíno." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/18/18158/tde-03102016-160420/.

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A terapia fotodinâmica (TFD) utilizando ácido 5-aminolevulinico (ALA) e derivados em aplicação tópica e, como precursor da protoporfirina IX (PPIX) apresenta alguns limitantes relativos a baixa permeação das substâncias na pele. Comportamento este que afeta a produção e homogeneidade da distribuição da PPIX na superfície e camadas mais profundas da pele. Para resolver essa limitação muitos autores propõem alternativas modificando a molécula do ALA e derivados, bem como modificando as propriedades químicas da fase externa da emulsão (mais hidrofílica ou hidrofóbica) ou então o sistema de entreg

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Walter, Vivien. "Lipid membrane interaction with self-assembling cell-penetrating peptides." Thesis, Strasbourg, 2017. http://www.theses.fr/2017STRAE032/document.

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Les peptides pénétrateurs de cellule (CPP) sont des oligopeptides cationiques faisant parti des vecteurs les plus étudiés dans le cadre du développement du transport ciblé de médicament à l’intérieur de l’organisme. Les applications principales sont par exemple le traitement des cancers ou la thérapie génique. Néanmoins, certaines caractéristiques des CPPs rendent leur utilisation médicale compliquée, tels que leur manque de spécificité à l’égard des cellules cibles ou la perte de leurs propriétés pénétrantes lorsqu’un cargo moléculaire leur est greffé. L’une des solutions envisagées pour réso

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Torella, Joseph Peter. "Confocal single-molecule fluorescence as a tool for investigating biomolecular dynamics in vitro and in vivo." Thesis, University of Oxford, 2011. http://ora.ox.ac.uk/objects/uuid:f57d1984-8db9-4d79-b333-f1be507ca3bf.

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Confocal single-molecule fluorescence is a powerful tool for monitoring conformational dynamics, and has provided new insight into the enzymatic activities of complex biological molecules such as DNA and RNA polymerases. Though useful, such studies are typically qualitative in nature, and performed almost exclusively in highly purified, in vitro settings. In this work, I focus on improving the methodology of confocal single-molecule fluorescence in two broad ways: (i) by enabling the quantitative identification of molecular dynamics in proteins and nucleic acids in vitro, and (ii) developing t

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Kao, Min-Tzu. "Nano-rubans et cristaux anisotropes d’anthracènes et tétracènes à émission accordable : étude de la photophysique et des transferts d’énergie par microscopie confocale de fluorescence." Thesis, Bordeaux 1, 2012. http://www.theses.fr/2012BOR14647/document.

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De nouveaux nano-objets anisotropes fluorescents sont obtenus par l’assemblage d’acènes spécifiquement conçus. Dans des cristaux, nano-rubans et nanoparticules anisotropes de 2,3-dialkyldiphenylanthracènes, les efficacités et la polarisation de l’émission bleue sont remarquables. La couleur de l’émission est accordée par le dopage avec des émetteurs verts et oranges (di- et tétra-phényltétracènes). La microscopie confocale de fluorescence permet d’étudier les cinétiques des états excités et des transferts d’énergie photo-induits, ainsi que la dispersion et les orientations des émetteurs. Pour

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Bridier, Arnaud. "Architecture des biofilms et résistance à la désinfection : apport de l'imagerie de fluorescence multimodale." Phd thesis, AgroParisTech, 2011. http://pastel.archives-ouvertes.fr/pastel-01002653.

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Dans les environnements naturels, industriels ou médicaux, les microorganismes sont majoritairement présents en étant associés aux surfaces dans des communautés hautement organisées appelées biofilms. Ces édifices biologiques constituent une stratégie de survie étonnement efficace témoignant d'une grande capacité de résistance à différent stress environnementaux tels que les traitements de nettoyage et de désinfection. L'impact des biofilms d'un point de vue sanitaire est donc considérable du fait qu'ils permettent la persistance et la transmission de germes pathogènes dans l'environnement. Da

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Zuo, Li. "Molecular Mechanisms of Stress-induced Reactive Oxygen Species Formation in Skeletal Muscle." The Ohio State University, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=osu1038853894.

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Solař, Jan. "Hodnocení migrace značených buněk v tkáni." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2016. http://www.nusl.cz/ntk/nusl-241961.

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This diploma thesis deals with analysing of modern methods for cell detection, visualization and quantification in 3D space. The first section deals with optical methods for cells detection. There is detailed discussion about cell labeling and detection on confocal microscopy. There is also description about developed algorithm for whole cell volume quantification from microscopy images. This could made a comparsion of fluorescence signal according to time of cell labeling and according to cell shapes. There was also optimalization of handmade tissue phantoms visualization. It could be compare

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Do, Le Duy. "Relation entre l’annexine A6 et la phospholipase D1 pendant le processus d’exocytose dans les cellules PC12." Thesis, Lyon 1, 2014. http://www.theses.fr/2014LYO10160/document.

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L'exocytose régulée, est un processus qui permet la communication entre les cellules à travers la sécrétion des hormones et des neurotransmetteurs. Dans les neurones et les cellules neuroendocrines, l'exocytose est strictement contrôlée par des signaux extracellulaires tels que le potentiel trans-membranaire et la fixation des ligands sur des récepteurs. Des progrès substantiels ont été effectués afin de comprendre le mécanisme moléculaire de l'exocytose. Les composants majeurs de la machinerie de sécrétion ont été dévoilés. Maintenant, la question qui émerge concerne le rôle de la plateforme

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Travascio, Francesco. "Modeling Molecular Transport and Binding Interactions in Intervertebral Disc." Scholarly Repository, 2009. http://scholarlyrepository.miami.edu/oa_dissertations/322.

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Low back pain represents a significant concern in the United States, with 70% of individuals experiencing symptoms at some point in their lifetime. Although the specific cause of low back pain remains unclear, symptoms have been strongly associated with degeneration of the intervertebral disc. Insufficient nutritional supply to the disc is believed to be a major mechanism for tissue degeneration. Understanding nutrients' transport in intervertebral disc is crucial to elucidate the mechanisms of disc degeneration, and to develop strategies for tissue repair (in vivo), and tissue engineering (in

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