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Tesis sobre el tema "Consensus sequence"

Autor: Grafiati

Publicado: 4 de junio de 2021

Última modificación: 25 de julio de 2025

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1

Wilson, Lindsay Anne. "The enterobacterial repeated intergenic consensus (ERIC) sequence." Thesis, University of Nottingham, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342441.

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2

Mokin, Sergey. "Measuring deviation from a deeply conserved consensus in protein multiple sequence alignments." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=21956.

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Proteins across species show variable degrees of conservation. Different patterns of conservation in the columns of an alignment indicate different evolutionary pressures on sequences. Protein conservation analysis is useful for a wide variety of applications, including disease mutation assessment, pseudogene analysis and functional residue prediction. This study describes a novel measure of column conservation in protein multiple sequence alignments (‘MSA'), and the application of this measure to calculate statistical deviation from alignment consensus (‘SDAC'). We have assessed SDAC for two

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3

Kamura, Eri. "Exploring the Methylation Status of RAI1 and the RAI1 Consensus Binding Sequence." VCU Scholars Compass, 2009. http://scholarscompass.vcu.edu/etd/1891.

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Smith Magenis Syndrome (SMS) is a multiple congenital anomalies/ mental retardation disorder caused by deletion or mutation of the RAI1 gene on chromosome 17p11.2. The majority of patients with SMS phenotypes have a deletion or mutation of RAI1. However, some patients have been observed with SMS-like phenotypes and yet have no deletions or mutations in the RAI1 gene. One possible explanation could be aberrant methylation of RAI1 since RAI1 is present and yet may be silenced. In order to study this possibility, patient cell lines were treated with 5-Aza-2’-deoxycytidine. RNA was extracted a

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4

Babst, Scheán. "Mitochondrial DNA consensus sequence for the Tswana population of South Africa / Scheán Babst." Thesis, North-West University, 2012. http://hdl.handle.net/10394/9110.

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Evolutionary studies are critical in eliciting the fundamental phylogeny within and among populations of living organisms. Genetic diversity is displayed in human mitochondrial DNA (mtDNA) as haplogroups that consist of shared mutations, which are carried to the following generation through the maternal lineage. The current haplogroup hierarchies commonly used to describe and compare the genetic diversity of global human populations are based on the available mtDNA sequence variation datasets of numerous continent-specific populations. The description of mtDNA variation in human populations is

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5

Mouton, Christa. "Mitochondrial genome consensus sequence for the South African Khoi-San population / Christa Mouton." Thesis, North-West University, 2003. http://hdl.handle.net/10394/9618.

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Maternal inheritance and the absence of recombination have contributed to mitochondrial deoxyribonucleic acid (mtDNA) being utilised to study human evolution. This, together with an increased mutation rate in mtDNA, provides information about the most recent common ancestor of modern humans. Previous studies suggested that Africa harbours the highest mtDNA diversity, supporting an out-of-Africa hypothesis for modern human evolution. From subsequent studies it was suggested that the Khoi-San population, in particularly the !Kung, cluster at the deepest root of the global phylogenetic tree. The

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6

Wynn, Anna. "Four differentially expressed cDNAs containing the Rebers-Riddiford consensus sequence in Callinectes sapidus /." Electronic version (PDF), 2004. http://dl.uncw.edu/etd/2004/wynna/annawynn.pdf.

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7

Ng, Harald. "Distributed Consensus: Performance Comparison of Paxos and Raft." Thesis, KTH, Skolan för elektroteknik och datavetenskap (EECS), 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-281973.

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With the growth of the internet, distributed systems have become increasingly important in order to provide more available and scalable applications. Con- sensus is a fundamental problem in distributed systems where multiple pro- cesses have to agree on the same proposed value in the presence of partial failures. Distributed consensus allows for building various applications such as lock services, configuration manager services or distributed databases.Two well-known consensus algorithms for building distributed logs are Multi-Paxos and Raft. Multi-Paxos was published almost three decades befo

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8

Wen, Meimei. "Structural studies of a consensus sequence peptide (CSP) ABAB of apolipoproteins through NMR spectroscopy." Thesis, Boston University, 2013. https://hdl.handle.net/2144/11084.

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Thesis (Ph.D.)--Boston University<br>The apolipoproteins play critical roles in lipid transport, lipid metabolism and the pathophysiology of dyslipoproteinemias, most importantly atherosclerosis. ApoA-1 is a representative member of the family of exchangeable apolipoproteins and the major apolipoprotein of high density lipoprotein (HDL). HDL is responsible for the pathway of reverse cholesterol transport and the only particle capable of removing cholesterol from peripheral cells for transport to the liver. The sequences ofthe exchangeable apolipoproteins contain 11/22 residue tandem sequence

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9

McMillen, Lyle, and l. mcmillen@sct gu edu au. "Isolation and Characterisation of the 5'-Nucleotidase from Escherichia coli." Griffith University. School of Biomolecular and Biomedical Science, 2001. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20030226.153545.

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Escherichia coli 5'-nucleotidase is a periplasmically localised enzyme capable of hydrolysing a broad range of substrates, including all 5'-ribo- and 5'-deoxyribonucleotides, uridine diphosphate sugars, and a number of synthetic substrates such as bis (r-nitrophenyl) phosphate. The enzyme has been shown to contain at least one zinc ion following purification, and to have two metal binding sites in the catalytic cleft. 5'-Nucleotidase activity is significantly stimulated by the addition of particular divalent metal ions, most notably cobalt which results in a 30-50 fold increase in activity.

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10

McMillen, Lyle. "Isolation and Characterisation of the 5'-Nucleotidase from Escherichia coli." Thesis, Griffith University, 2001. http://hdl.handle.net/10072/366487.

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Escherichia coli 5'-nucleotidase is a periplasmically localised enzyme capable of hydrolysing a broad range of substrates, including all 5'-ribo- and 5'-deoxyribonucleotides, uridine diphosphate sugars, and a number of synthetic substrates such as bis (r-nitrophenyl) phosphate. The enzyme has been shown to contain at least one zinc ion following purification, and to have two metal binding sites in the catalytic cleft. 5'-Nucleotidase activity is significantly stimulated by the addition of particular divalent metal ions, most notably cobalt which results in a 30-50 fold increase in activity.

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11

Brown, Andrew S. "Identification of a phospho-hnRNP E1 Nucleic Acid Consensus Sequence Mediating Epithelial to Mesenchymal Transition." Kent State University / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=kent1437943957.

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12

Mohan, Sidharth. "Consensus, Correlation And Combinatorics Based Approaches In Engineering And Exploring Triosephosphate Isomerase Stability." The Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1503054678218166.

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13

Li, Chunjie. "Development of a FRET biosensor for ROCK based on a consensus substrate sequence identified by KISS technology." 京都大学 (Kyoto University), 2017. http://hdl.handle.net/2433/225755.

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14

Koekemoer, Michelle. "Construction of a mitochondrial consensus sequence for the Khoi-San population of Southern Africa / by Michelle Koekemoer." Thesis, North-West University, 2010. http://hdl.handle.net/10394/4397.

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The revised Cambridge Reference Sequence (rCRS) is used as a standard for the human mitochondrial DNA (mtDNA) sequence in studies of human evolution and the identification of disease-causing mutations. Due to the large number of differences observed between the rCRS and mitochondrial sequences obtained from individuals of African descent, it is frequently difficult to differentiate between alterations that are population-specific or have possible pathological significance. To address this problem, two human consensus sequences consisting of mitochondrial sequences from different continents and

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15

Barozzi, I. G. "OVERLAPPING SEQUENCE FEATURES OF MAMMALIAN ENHANCERS COORDINATELY CONTROL ENGAGEMENT OF TRANSCRIPTION FACTOR CONSENSUS SITES AND NUCLEOSOMAL OCCUPANCY." Doctoral thesis, Università degli Studi di Milano, 2014. http://hdl.handle.net/2434/234131.

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In mammalian cells transcription factors (TFs) bind only to a small fraction of the available consensus sites in the genome. In particular, they prefer sites embedded in regions of computationally predicted high nucleosomal occupancy. This is compatible with non-exclusive mechanisms of nucleosome-driven TF-binding and nucleosome-mediated masking of TF binding sites, suggesting that TFs, and in particular pioneers, must overcome a strong barrier in order to engage binding. Exploiting the available information for the hematopoietic master regulator Pu.1, we applied machine learning approaches an

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16

Rossl, Anthony. "A Synthetic Acetylation Substrate to Study Gcn5 Targeting and Function in Yeast." Thesis, Université d'Ottawa / University of Ottawa, 2018. http://hdl.handle.net/10393/38300.

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Acetylation was previously thought to occur exclusively on histones, but recent high-throughput screens have identified thousands of non-histone substrates. Despite the identification of these sites, little is known about how these acetyltransferase enzymes target their substrates. Gcn5 is the catalytic acetyltransferase found within the highly conserved SAGA complex. Recently, a member of this complex, Ada2, was found to impact Gcn5 substrate selection. In the yeast model organism Saccharomyces cerevisiae, a synthetic substrate developed from a proposed Gcn5-specific consensus sequence is use

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17

Tanaka, Emi. "Statistical Methods for Improving Motif Evaluation." Thesis, The University of Sydney, 2014. http://hdl.handle.net/2123/13922.

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Gene regulation, especially cis-regulation of gene expression by the binding of transcription factors, is a critical component of cellular physiology. Transcription regulation is heavily influenced by the binding of transcription factors, and as such, it is of great interest to characterise these binding sites. The binding sites of a transcription factor are collectively referred to as a regulatory motif. Recent advancement in sequencing technology generated vast amounts of biological data. Thus computational tools are required to process and analyse this massive information. In particular, c

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18

CHALAOUX, FRANCOIS-REGIS. "Etude conformationnelle d'oligopeptides porteurs de la sequence consensus rxvrg, site d'action d'une endoprotease de la peau de xenopus laevis." Paris 6, 1994. http://www.theses.fr/1994PA066783.

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Le but de l'etude presentee dans ce document est de montrer l'apport de la determination de la structure tridimensionnelle en solution d'une serie d'oligopeptides lineaires contenant la sequence consensus rxvrg a la comprehension de leur maturation par l'endoprotease-rxvrg de la peau de xenopus laevis. En effet, les proteases impliquees dans les processus de maturation des precurseurs et porteurs de ces oligopeptides necessitent un site de coupure tres specifique. Cette specificite doit etre necessairement associee a l'existence de caracteristiques sequentielles et/ou conformationnelles permet

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19

Nilsson, Johan. "Membrane protein topology : prediction, experimental mapping and genome-wide analysis /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7349-963-3/.

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20

DANCKAERT, PERRUCHOT ANNE. "Traitement informatique des autoradiographies de gels de sequences : conception et mise en oeuvre d'un systeme automatique d'interpretation d'autoradiographies, elaboration d'un algorithme de construction de la sequence consensus a partir de fragments." Paris 7, 1988. http://www.theses.fr/1988PA077045.

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21

MILHE, CATHERINE. "Flexibilite de l'adn : etude par rmn d'oligonucleotides en solution. structure et dynamique de points chauds de mutagenese et dynamique de la sequence consensus d'un promoteur." Université Louis Pasteur (Strasbourg) (1971-2008), 1994. http://www.theses.fr/1994STR13137.

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Ce travail porte sur l'etude structurale et dynamique, par resonance magnetique nucleaire (rmn), de sequences d'adn en solution. L'acetylaminofluorene (aaf) est un puissant cancerigene qui se fixe principalement en position c8 des guanines. Il induit, avec des frequences elevees, des mutations par deletion de 1 ou 2 paires de bases en des points chauds de mutagenese particuliers. La mise en place de la mutation pourrait intervenir soit par l'action d'un groupe d'enzymes qui reconnaitrait, sur l'adn endommage, une structure particuliere, soit au cours de la replication de l'adn avec formation d

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22

Malaby, Heidi L. H. "Identification of Molecular Determinants that Shift Co- and Post-Translational N-Glycosylation Kinetics in Type I Transmembrane Peptides: A Dissertation." eScholarship@UMMS, 2014. https://escholarship.umassmed.edu/gsbs_diss/702.

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Asparagine (N)-linked glycosylation occurs on 90% of membrane and secretory proteins and drives folding and trafficking along the secretory pathway. The N-glycan can be attached to an N-X-T/S-Y (X,Y ≠ P) consensus site by one of two oligosaccharyltransferase (OST) STT3 enzymatic isoforms either during protein translation (co-translational) or after protein translation has completed (post-translational). While co-translational N-glycosylation is both rapid and efficient, post-translational N-glycosylation occurs on a much slower time scale and, due to competition with protein degradation and fo

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23

Malaby, Heidi L. H. "Identification of Molecular Determinants that Shift Co- and Post-Translational N-Glycosylation Kinetics in Type I Transmembrane Peptides: A Dissertation." eScholarship@UMMS, 2004. http://escholarship.umassmed.edu/gsbs_diss/702.

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Asparagine (N)-linked glycosylation occurs on 90% of membrane and secretory proteins and drives folding and trafficking along the secretory pathway. The N-glycan can be attached to an N-X-T/S-Y (X,Y ≠ P) consensus site by one of two oligosaccharyltransferase (OST) STT3 enzymatic isoforms either during protein translation (co-translational) or after protein translation has completed (post-translational). While co-translational N-glycosylation is both rapid and efficient, post-translational N-glycosylation occurs on a much slower time scale and, due to competition with protein degradation and fo

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24

Rajbhandari, Rajita [Verfasser], and Mathias [Akademischer Betreuer] Herrmann. "Studies on the role of the putative phosphorylation consensus sequence YXNX in the tandem repeat domains of the Staphylococcus aureus Mu50 Extracellular Adherence Protein (Eap) / Rajita Rajbhandari. Betreuer: Mathias Herrmann." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2012. http://d-nb.info/1052222625/34.

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25

Vázquez-Figueroa, Eduardo. "Development of a novel dehydrogenase and a stable cofactor regeneration system." Diss., Georgia Institute of Technology, 2008. http://hdl.handle.net/1853/31685.

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The first goal of this work focused on the development of an amine dehydrogenase (AmDH) from a leucine dehydrogenase using site-directed mutagenesis. We aimed at reductively aminating a prochiral ketone to a chiral amine by using leucine dehydrogenase (LeuDH) as a starting template. This initial work was divided into two stages. The first focused mutagenesis to a specific residue (K68) that we know is key to developing the target functionality. Subsequently, mutagenesis focused on residues known to be in close proximity to a key region of the substrate (M65 and K68). This approach allowed

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26

Vargas, Jesse. "Protein-DNA interactions : characterization of a definitive DNA binding sequence consensus for full length mammalian DEAF-1 and determination of the DNA binding potential of the tumor metastasis related protein, MTA1 /." Available to subscribers only, 2007. http://proquest.umi.com/pqdweb?did=1483331711&sid=2&Fmt=2&clientId=1509&RQT=309&VName=PQD.

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27

Liu, Chong. "EVIDENCE FOR THE MATURATION OF CELLULAR IMMUNE RESPONSES IN EQUINE INFECTIOUS ANEMIA VIRUS-INFECTED PONIES." UKnowledge, 2013. http://uknowledge.uky.edu/gluck_etds/10.

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Equine infectious anemia virus (EIAV) has been used as a model to investigate protective mechanisms against lentiviruses. Unlike other lentiviruses, EIAV replication can be eventually controlled in most infected horses leading to an inapparent carrier state free of overt clinical signs which can last for many years. Maintenance of this carrier state is absolutely dependent on active immune responses as evidenced by the fact that immunosuppressive drugs can induce the recurrence of disease. However, the immune mechanisms that are responsible for this control of infection are not yet identified.

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28

Kao, Daniel Joseph. "Development of a synthetic peptide vaccine and antibody therapeutic for the prevention and treatment of Pseudomonas Aeruginosa infection /." Connect to full text via ProQuest. Limited to UCD Anschutz Medical Campus, 2007.

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Thesis (Ph.D. in Pharmacology) -- University of Colorado Denver, 2007.<br>Typescript. Includes bibliographical references (leaves 203-212; 260-261). Free to UCD affiliates. Online version available via ProQuest Digital Dissertations;

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29

Bellora, Pereyra Nicolás. "In silico analysis of regulatory motifs in gene promoters." Doctoral thesis, Universitat Pompeu Fabra, 2010. http://hdl.handle.net/10803/7202.

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Regulation of gene transcription is a complex process involving many different proteins, some of which bind in a sequence-specific manner to DNA motifs in the gene promoter. The need to maintain specific interactions between transcription factors and proteins involved in the RNA polymerase II complex is expected to impose constrains on the relative position and spacing of the interacting DNA motifs. The present work includes the development of a novel approach to identify motifs that show a preferential location in DNA sequences and the implementation of a public web application called PEAKS.

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30

Mlera, Luwanika. "Preparatory investigations for developing a transcript-based rotavirus reverse genetics system / Luwanika Mlera." Thesis, North-West University, 2012. http://hdl.handle.net/10394/9523.

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Reverse genetics systems that are based on either viral transcripts or cDNA genome segments cloned in plasmids have recently been reported for some of the dsRNA viruses of the Reoviridae family, namely African horsesickness virus, bluetongue virus and orthoreovirus. For rotaviruses, three reverse genetics systems which only allow the manipulation of a single genome segment have been described. These rotavirus single genome segment reverse genetics systems are not true stand-alone systems because they require a helper virus and a recombinant virus selection step. A true selection-free, plasmid-

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31

Liang, Chengzhi. "COPIA: A New Software for Finding Consensus Patterns in Unaligned Protein Sequences." Thesis, University of Waterloo, 2001. http://hdl.handle.net/10012/1050.

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Consensus pattern problem (CPP) aims at finding conserved regions, or motifs, in unaligned sequences. This problem is NP-hard under various scoring schemes. To solve this problem for protein sequences more efficiently,a new scoring scheme and a randomized algorithm based on substitution matrix are proposed here. Any practical solutions to a bioinformatics problem must observe twoprinciples: (1) the problem that it solves accurately describes the real problem; in CPP, this requires the scoring scheme be able to distinguisha real motif from background; (2) it provides an efficient algorithmt

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32

Almeida, Rafael Ribeiro. "Reconhecimento entre clados e efeito supressor induzido por vacinas de DNA codificando peptídeos conservados e promíscuos do grupo M do HIV-1." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/5/5146/tde-29102014-160344/.

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A busca por uma construção vacinal contra o HIV-1 é urgente. Os linfócitos T CD4+ têm assumido um papel de destaque no campo de vacinas por participar no controle da replicação do HIV-1, seja auxiliando as funções efetoras de linfócitos T CD8+ e a produção de anticorpos por linfócitos B ou mesmo agindo de forma citotóxica sobre macrófagos infectados. A utilização de sequências consenso do grupo M do HIV-1 é apontada como uma das maneiras de se contornar os problemas relacionados à diversidade viral. Além disso, é preciso construir vacinas que apresentem potencial de induzir respostas imunes co

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33

Clarke, Andrew, Stefan Prost, Jo-Ann Stanton, et al. "From cheek swabs to consensus sequences: an A to Z protocol for high-throughput DNA sequencing of complete human mitochondrial genomes." BioMed Central, 2014. http://hdl.handle.net/10150/610024.

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BACKGROUND:Next-generation DNA sequencing (NGS) technologies have made huge impacts in many fields of biological research, but especially in evolutionary biology. One area where NGS has shown potential is for high-throughput sequencing of complete mtDNA genomes (of humans and other animals). Despite the increasing use of NGS technologies and a better appreciation of their importance in answering biological questions, there remain significant obstacles to the successful implementation of NGS-based projects, especially for new users.RESULTS:Here we present an 'A to Z' protocol for obtaining comp

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34

Moore, Siobhan E. "Fertility practitioners' experience of the psychological sequelae of unmet parental goals after unsuccessful fertility treatment : a Delphi Consensus Study." Thesis, Cardiff University, 2018. http://orca.cf.ac.uk/115247/.

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This thesis investigates the adjustment processes to involuntary childlessness and the psychological distress associated with unmet parental goals. Paper one reports a systematic review of quantitative studies looking at how trauma theory informs the clinical understanding of adjustment to involuntary childlessness. This yielded eight studies which were reviewed and rated using a quality appraisal tool. The data extracted, focused on the prevalence of trauma and post traumatic growth to infertility. No studies included, focused their research on cohorts of women who identified as being involun

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35

Chu, Huan-Lin, and 朱煥霖. "Mining Consensus Patterns Across Heterogeneous Sequence Databases." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/zf9kav.

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碩士<br>國立中正大學<br>資訊管理系研究所<br>104<br>Many modern enterprise collect large quantities of data, such as customer preference or their temporal purchasing behaviors, and utilize data mining as their competitive advantages. However, some conflicts in the data may exist, and determining how to aggregate many different opinions into a consensus is a traditional core problem in recommendation systems and decision support systems. Taking the preference ranking problem as an example, u_1 : (A ≥ B ≻ C) indicates that for the user u_1, (1)A is at least favorable than B; (2)B is more favorable compared to C.

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36

劉玉山. "Using Tabu Search for Finding Consensus Sequence." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/07094808649625506348.

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碩士<br>樹德科技大學<br>資訊管理研究所<br>92<br>A consensus sequence captures the common features of a set of sequences. Consensus sequences are important in various DNA sequencing applications and are a convenient way to characterize a family of molecules. The search of such a sequence is an NP-Complete problem and, therefore, no efficient algorithms to search the sequence can be designed. Tabu Search has been used in a wide variety of applications to find solutions for NP-Complete problems. In this paper, we use a variant of Tabu search to find a consensus sequence. The method may be viewed as consisting o

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37

Chang, Yi-Feng, and 張益峰. "Human Promoter Prediction with Extracted Consensus Sequence Patterns." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/73014006863284314043.

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碩士<br>朝陽科技大學<br>資訊管理系碩士班<br>91<br>Promoter region is a DNA sequence that is usually located on the upstream of the transcriptional starting site (TSS) of a gene. If the hallmarks of the known promoter sequences can be extracted, we can use these hallmarks to recognize unknown promoter regions from un-labeled genome sequences. Furthermore, we can indirectly identify the potential TSSs of genes, and then the estimated thirty to fifty thousand of genes can be predicted and explored. Up to present, there are many announced promoter sequences that were discovered by molecular biologists with biolog

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38

Chan, Viann Wendy. "Comparative sequence analysis : prediction of consensus Xist RNA secondary structure." Thesis, 2003. http://hdl.handle.net/2429/14473.

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The typical female mammal has two X chromosomes. However, only one copy remains active, while the other copy is silenced. The Xist gene has been found to remain active on the silenced X chromosome. Other lab experiments have determined that the Xist RNA plays a significant role in the process of X - chromosome inactivation. Our goal in this thesis is to predict a possible consensus structure for the Xist RNA using comparative sequence analysis. Since the Xist RNA structure is not known, we evaluate our approaches on sequences with known structures. There are three main phases for this approach

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黃自健. "Identification Of A Consensus Sequence Motif For Autophosphorylation-Dependent Protein Kinase." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/30520107396656820090.

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碩士<br>國立清華大學<br>生物醫學研究所<br>82<br>In a previous report (Yang et al., (1987a) J. Biol. Chem. 262, 7034 - 7040), a cyclic AMP and calcium - independent brain kinase which requires autophosphorylation for activity was identified as a very potent myelin basic protein (MBP) kinase. In this report, the phosphorylation sites of MBP by this autophosphorylation - dependent protein kinase (auto - kinase) were further determined by two - dimensional electrophoresis / thin - layer chromatography, phosphoamino acid analysis, high performance liquid chromatography, tryptic peptide mapping, sequential manual

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40

Kolychev, Dmitri. "Microsatellite detection and consensus sequence verification by virtual PCR and machine learning." 2003. http://purl.galileo.usg.edu/uga%5Fetd/kolychev%5Fdmitri%5Fs%5F200308%5Fms.

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41

Woodworth, AM. "Gene regulation by RUNX1 in the absence of consensus sequences." Thesis, 2020. https://eprints.utas.edu.au/35253/1/Woodworth_whole_thesis.pdf.

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Runt-related transcription factor 1 (RUNX1) is a transcription factor that has an important role in haematopoietic cell development and function and is frequently disrupted in leukaemia. RUNX1 is commonly described as a sequence-specific DNA binding factor which recognises the consensus sequence TG(T/C)GGT in the promoter and enhancer regions of its target genes to affect changes in gene expression. However, the advent of techniques to study DNA-protein interactions on a genome-wide scale has provided the opportunity to re-assess RUNX1 localisation and function, and this analysis suggests that

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42

Sung, Wen-Long, and 孫文隆. "Identification of the consensus sequence of the SOS box in Xanthomonas axonopodis pv. citri." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/58471540096145062408.

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碩士<br>輔仁大學<br>生命科學系碩士班<br>93<br>There are two lexA genes in Xanthomonas axonopodis pv. citri. LexA1 and LexA2 proteins, which are supposed to function as a repressor and modulate many DNA repair genes. By analyzing two sequences of LexA proteins, we discover they are not the same as the relative sequences (α domain) that bind to DNA. Therefore, we assume that the two proteins bind to different DNA sequences. In order to get further evidence, Electrophoretic mobility shift assay (EMSA) is adopted. The result shows that LexA1 protein can bind to lexA1 promoter (nucleotides -40 to +155, relative

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Cheng, Li-chen, and 鄭麗珍. "Mining maximum consensus sequences from group ranking data." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/58303646266345835813.

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博士<br>國立中央大學<br>資訊管理研究所<br>96<br>In the last decade, the problem of getting a consensus group ranking from users’ ranking data has received increased attention due to its widespread applications. Previous research solved this problem by consolidating the opinions of all users, thereby obtaining an ordering list of all items that represent the achieved consensus. The weakness of this approach, however, is that it always produces a ranking list of all items, regardless of how many conflicts exist among users. This work rejects the forced agreement of all items. Instead, we define a new concept,

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Chang, Jerry, and 張立群. "Consensus Cis-sequences of Purified Trans-factors from Yeast Expression System." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/97186947453766587193.

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碩士<br>中國文化大學<br>生物科技研究所<br>93<br>The goal of this thesis is to establish fast and global analysis platform of differential regulomics , it continue futher study with probe of labeling human tumor cell line to screening human liver complementary nucleic acid from high expressing and specific yeast yield to obtain Trans -factors, and then utilize existing Trans -factors selection regulation relevantly to the Cis element of antitumor gene p53 by building and constructing the screening regulomics technological integrality entirely. This research is purification transcription factor p53 protein fr

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Wang, Pei-Hsueh, and 王碧雪. "Yeast One-Hybrid Screening System forFinding Consensus Cis-sequences of Expressed Trans-factors." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/39649472719114590968.

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碩士<br>中國文化大學<br>生物科技研究所<br>93<br>Summary The goal in research of this thesis, in order to use known p53 protein to prove that innovate the technological platform, combine the saccharomycete form and mix the system of shutting (Yeast One-Hybrid System) increase and choose with the circulation type goal (Cyclic Amplification and Selection of Targets, the mutual experiment application that CASTing ), fetch its safe effective advantage and obtain specific p53 protein uniqueness and combine the gene order, use the transcription factor activating area of p53 to form the characteristic with the singl

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Hsu, Chien-Hsiu, and 徐千琇. "Identification of the consensue sequence of LexA binding site in Xanthomonas campestris pv. citri." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/61257460262365186230.

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碩士<br>輔仁大學<br>生物學系<br>90<br>Abstract A Xanthomonas campestris gene cluster consisted of lexA, recA and recX genes was identified and characterized. Electrophoretic mobility shift analysis indicated that the LexA protein of X. c. pv. citri bind to the lexA and recA promoters of X. c. pv. campestris and X. oryzae. pv. oryzae and repress the Xanthomonas campestris recA gene, indicating that the X. c. pv. citri LexA protein was functional in different Xanthomonas campestris pathovars. It was confirmed that a symmetrical sequence of TTAGTAGTAATACTACTAA within the lexA promoter region is

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Wentzel, Johannes Frederik. "Investigating the importance of co-expressed rotavirus proteins in the development of a selection-free rotavirus reverse genetics system / Johannes Frederik Wentzel." Thesis, 2014. http://hdl.handle.net/10394/12270.

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Reverse genetics is an innovative molecular biology tool that enables the manipulation of viral genomes at the cDNA level in order to generate particular mutants or artificial viruses. The reverse genetics system for the influenza virus is arguably one of the best illustrations of the potential power of this technology. This reverse genetics system is the basis for the ability to regularly adapt influenza vaccines strains. Today, reverse genetic systems have been developed for many animal RNA viruses. Selection-free reverse genetics systems have been developed for the members of the Reoviridae

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