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1

Cutarelli, Anna, Francesca Carella, Francesca De Falco, et al. "Detection and Quantification of Nocardia crassostreae, an Emerging Pathogen, in Mytilus galloprovincialis in the Mediterranean Sea Using Droplet Digital PCR." Pathogens 12, no. 8 (2023): 994. http://dx.doi.org/10.3390/pathogens12080994.

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Nocardia crassostreae is a novel pathogen responsible for infections in oysters (Crassostrea gigas) and mussels (Mytilus galloprovincialis). N. crassostreae is also responsible for nocardiosis both in immunocompetent and immunocompromised patients. We investigated N. crassostreae DNA in mussels grown in marine sites of the Mediterranean Sea in the Campania Region. We examined 185 mussel pooled samples by droplet digital PCR (ddPCR) and real-time quantitative PCR (qPCR), each pool composed of 10 mussels and 149 individual mussels. ddPCR detected N. crassostreae DNA in 48 mussel pooled samples a
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2

Elston, Ralph A. "Infectious diseases of the Pacific oyster, Crassostrea gigas." Annual Review of Fish Diseases 3 (January 1993): 259–76. http://dx.doi.org/10.1016/0959-8030(93)90038-d.

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3

Schlenk, Daniel, and Donald R. Buhler. "Xenobiotic biotransformation in the pacific oyster (Crassostrea gigas)." Comparative Biochemistry and Physiology Part C: Comparative Pharmacology 94, no. 2 (1989): 469–75. http://dx.doi.org/10.1016/0742-8413(89)90100-x.

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4

Lacoste, Arnaud, Shelagh K. Malham, Florence Gélébart, Anne Cueff, and Serge A. Poulet. "Stress-induced immune changes in the oyster Crassostrea gigas." Developmental & Comparative Immunology 26, no. 1 (2002): 1–9. http://dx.doi.org/10.1016/s0145-305x(01)00067-2.

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5

Sillanpää, J. Kirsikka, Henrik Sundh, and Kristina S. Sundell. "Calcium transfer across the outer mantle epithelium in the Pacific oyster, Crassostrea gigas." Proceedings of the Royal Society B: Biological Sciences 285, no. 1891 (2018): 20181676. http://dx.doi.org/10.1098/rspb.2018.1676.

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Calcium transport is essential for bivalves to be able to build and maintain their shells. Ionized calcium (Ca 2+ ) is taken up from the environment and eventually transported through the outer mantle epithelium (OME) to the shell growth area. However, the mechanisms behind this process are poorly understood. The objective of the present study was to characterize the Ca 2+ transfer performed by the OME of the Pacific oyster, Crassostrea gigas, as well as to develop an Ussing chamber technique for the functional assessment of transport activities in epithelia of marine bivalves. Kinetic studies
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6

Potts, Robert W. A., Alejandro P. Gutierrez, Yennifer Cortés-Araya, Ross D. Houston, and Tim P. Bean. "Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas." PeerJ 8 (June 1, 2020): e9180. http://dx.doi.org/10.7717/peerj.9180.

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Cell culture provides useful model systems used in a wide range of biological applications, but its utility in marine invertebrates is limited due to the lack of immortalised cell lines. Primary cell and tissue cultures are typically used but remain poorly characterised for oysters, which can cause issues with experimental consistency and reproducibility. Improvements to methods of repeatable isolation, culture, and characterisation of oyster cells and tissues are required to help address these issues. In the current study, systematic improvements have been developed to facilitate the culture
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7

Bachère, E., D. Hervio, E. Mialhe, and H. Grizel. "Evidence of neutralizing activity against T3 coliphage in oyster Crassostrea gigas hemolymph." Developmental & Comparative Immunology 14, no. 3 (1990): 261–68. http://dx.doi.org/10.1016/0145-305x(90)90017-9.

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8

Tirapé, Ana, Coralie Bacque, Raphaël Brizard, Franck Vandenbulcke, and Viviane Boulo. "Expression of immune-related genes in the oyster Crassostrea gigas during ontogenesis." Developmental & Comparative Immunology 31, no. 9 (2007): 859–73. http://dx.doi.org/10.1016/j.dci.2007.01.005.

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9

Wang, Weilin, and Linsheng Song. "The diverse functions of DM9CPs in immune response of oyster Crassostrea gigas." Developmental & Comparative Immunology 148 (November 2023): 105006. http://dx.doi.org/10.1016/j.dci.2023.105006.

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10

Waechter, Magali, Frédérique Le Roux, Jean-Louis Nicolas, Éric Marissal, and Franck Berthe. "Caractérisation de bactéries pathogènes de naissain d’huître creuse Crassostrea gigas." Comptes Rendus Biologies 325, no. 3 (2002): 231–38. http://dx.doi.org/10.1016/s1631-0691(02)01428-2.

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11

Song, Ying, Xiaorui Song, Dan Zhang, Ying Yang, Lingling Wang, and Linsheng Song. "An HECT domain ubiquitin ligase CgWWP1 regulates granulocytes proliferation in oyster Crassostrea gigas." Developmental & Comparative Immunology 123 (October 2021): 104148. http://dx.doi.org/10.1016/j.dci.2021.104148.

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12

Qiao, Xue, Yanan Zong, Zhaoqun Liu, et al. "A novel CgIFNLP receptor involved in regulating ISG expression in oyster Crassostrea gigas." Developmental & Comparative Immunology 124 (November 2021): 104206. http://dx.doi.org/10.1016/j.dci.2021.104206.

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13

Bachère, Evelyne, Dominique Chagot, and Henri Grizel. "Separation of Crassostrea gigas hemocytes by density gradient centrifugation and counterflow centrifugal elutriation." Developmental & Comparative Immunology 12, no. 3 (1988): 549–59. http://dx.doi.org/10.1016/0145-305x(88)90071-7.

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14

Jia, Yunke, Bin Yang, Wenjing Dong, et al. "A serotonin receptor (Cg5-HTR-1) mediating immune response in oyster Crassostrea gigas." Developmental & Comparative Immunology 82 (May 2018): 83–93. http://dx.doi.org/10.1016/j.dci.2017.12.029.

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15

Jin, Yuhao, Xue Qiao, Xiaojing Lv, et al. "A conserved RNAi molecule Ago2 involved in antiviral immunity of oyster Crassostrea gigas." Developmental & Comparative Immunology 142 (May 2023): 104668. http://dx.doi.org/10.1016/j.dci.2023.104668.

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16

Terahara, Kazutaka, Keisuke G. Takahashi, and Katsuyoshi Mori. "Apoptosis by RGD-containing peptides observed in hemocytes of the Pacific oyster, Crassostrea gigas." Developmental & Comparative Immunology 27, no. 6-7 (2003): 521–28. http://dx.doi.org/10.1016/s0145-305x(02)00159-3.

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17

Barreau-Roumiguière, Catherine, Caroline Montagnani, and Jean-Michel Escoubas. "Characterization of a Tal/SCL-like transcription factor in the pacific oyster Crassostrea gigas." Developmental & Comparative Immunology 27, no. 9 (2003): 793–800. http://dx.doi.org/10.1016/s0145-305x(03)00075-2.

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18

Hervio, D., E. Bachere, E. Mialhe, and H. Grizel. "10.3 Chemiluminescent responses of Ostrea edulis and Crassostrea gigas hemocytes to Bonamia ostreae (Ascetospora)." Developmental & Comparative Immunology 13, no. 4 (1989): 449. http://dx.doi.org/10.1016/0145-305x(89)90176-6.

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19

Olafsen, Jan A., Thelma C. Fletcher, and Patrick T. Grant. "Agglutinin activity in pacific oyster (Crassostrea gigas) hemolymph following in vivo Vibrio anguillarum challenge." Developmental & Comparative Immunology 16, no. 2-3 (1992): 123–38. http://dx.doi.org/10.1016/0145-305x(92)90013-3.

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20

Thomas-Guyon, Hélène, Béatrice Gagnaire, Anne Bado-Nilles, Karine Bouilly, Sylvie Lapègue, and Tristan Renault. "Detection of phenoloxidase activity in early stages of the Pacific oyster Crassostrea gigas (Thunberg)." Developmental & Comparative Immunology 33, no. 5 (2009): 653–59. http://dx.doi.org/10.1016/j.dci.2008.11.011.

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21

Sun, Ying, Zhi Zhou, Lingling Wang, Chuanyan Yang, Shuai Jianga, and Linsheng Song. "The immunomodulation of a novel tumor necrosis factor (CgTNF-1) in oyster Crassostrea gigas." Developmental & Comparative Immunology 45, no. 2 (2014): 291–99. http://dx.doi.org/10.1016/j.dci.2014.03.007.

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22

Li, Yiqun, Shuai Jiang, Meijia Li, et al. "A cytokine-like factor astakine accelerates the hemocyte production in Pacific oyster Crassostrea gigas." Developmental & Comparative Immunology 55 (February 2016): 179–87. http://dx.doi.org/10.1016/j.dci.2015.10.025.

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23

Song, Kai, Yingxiang Li, Baoyu Huang, Li Li, and Guofan Zhang. "Genetic and evolutionary patterns of innate immune genes in the Pacific oyster Crassostrea gigas." Developmental & Comparative Immunology 77 (December 2017): 17–22. http://dx.doi.org/10.1016/j.dci.2017.07.012.

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24

Yang, Yuehong, Xue Qiao, Simiao Yu, et al. "A trace amine associated receptor mediates antimicrobial immune response in the oyster Crassostrea gigas." Developmental & Comparative Immunology 156 (July 2024): 105171. http://dx.doi.org/10.1016/j.dci.2024.105171.

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25

Leung, P. S. C., and K. H. Chu. "cDNA cloning and molecular identification of the major oyster allergen from the Pacific oyster Crassostrea gigas." Clinical & Experimental Allergy 31, no. 8 (2001): 1287–94. http://dx.doi.org/10.1046/j.1365-2222.2001.01165.x.

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26

Seo, Jung-Kil, Min Jeong Lee, Hye-Jin Go, et al. "Purification and antimicrobial function of ubiquitin isolated from the gill of Pacific oyster, Crassostrea gigas." Molecular Immunology 53, no. 1-2 (2013): 88–98. http://dx.doi.org/10.1016/j.molimm.2012.07.003.

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27

Bachere, E., D. Hervio, E. Mialhe, and H. Grizel. "4.6 Evidence for a neutralizing activity against T3 coliphage in the Crassostrea gigas oyster hemolymph." Developmental & Comparative Immunology 13, no. 4 (1989): 384. http://dx.doi.org/10.1016/0145-305x(89)90096-7.

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28

Roberts, Steven, Yannick Gueguen, Julien de Lorgeril, and Frederick Goetz. "Rapid accumulation of an interleukin 17 homolog transcript in Crassostrea gigas hemocytes following bacterial exposure." Developmental & Comparative Immunology 32, no. 9 (2008): 1099–104. http://dx.doi.org/10.1016/j.dci.2008.02.006.

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29

Itoh, Naoki, Ryo Kamitaka, Keisuke G. Takahashi та Makoto Osada. "Identification and characterization of multiple β-glucan binding proteins in the Pacific oyster, Crassostrea gigas". Developmental & Comparative Immunology 34, № 4 (2010): 445–54. http://dx.doi.org/10.1016/j.dci.2009.12.003.

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30

Liu, Zhaoqun, Zhi Zhou, Lingling Wang, et al. "The immunomodulation mediated by a delta-opioid receptor for [Met5]-enkephalin in oyster Crassostrea gigas." Developmental & Comparative Immunology 49, no. 2 (2015): 217–24. http://dx.doi.org/10.1016/j.dci.2014.11.017.

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31

Xu, Jiachao, Shuai Jiang, Yiqun Li, et al. "Caspase-3 serves as an intracellular immune receptor specific for lipopolysaccharide in oyster Crassostrea gigas." Developmental & Comparative Immunology 61 (August 2016): 1–12. http://dx.doi.org/10.1016/j.dci.2016.03.015.

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32

Lu, Guangxia, Zichao Yu, Mengmeng Lu, et al. "The self-activation and LPS binding activity of executioner caspase-1 in oyster Crassostrea gigas." Developmental & Comparative Immunology 77 (December 2017): 330–39. http://dx.doi.org/10.1016/j.dci.2017.09.002.

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33

Liu, Zhaoqun, Lingling Wang, Yunchen Yan, et al. "D1 dopamine receptor is involved in shell formation in larvae of Pacific oyster Crassostrea gigas." Developmental & Comparative Immunology 84 (July 2018): 337–42. http://dx.doi.org/10.1016/j.dci.2018.03.009.

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34

Lv, Zhao, Limei Qiu, Zhaoqun Liu, et al. "Molecular characterization of a cathepsin L1 highly expressed in phagocytes of pacific oyster Crassostrea gigas." Developmental & Comparative Immunology 89 (December 2018): 152–62. http://dx.doi.org/10.1016/j.dci.2018.08.014.

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35

Wang, Weilin, Xiaojing Lv, Zhaoqun Liu, et al. "The sensing pattern and antitoxic response of Crassostrea gigas against extracellular products of Vibrio splendidus." Developmental & Comparative Immunology 102 (January 2020): 103467. http://dx.doi.org/10.1016/j.dci.2019.103467.

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36

Li, Meijia, Miren Dong, Weilin Wang та ін. "A membrane-bound dopamine β-hydroxylase highly expressed in granulocyte of Pacific oyster Crassostrea gigas". Developmental & Comparative Immunology 104 (березень 2020): 103563. http://dx.doi.org/10.1016/j.dci.2019.103563.

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37

Lv, Xiaojing, Weilin Wang, Qi Zhao, et al. "A truncated intracellular Dicer-like molecule involves in antiviral immune recognition of oyster Crassostrea gigas." Developmental & Comparative Immunology 116 (March 2021): 103931. http://dx.doi.org/10.1016/j.dci.2020.103931.

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38

Liu, Yu, Weilin Wang, Qi Zhao, et al. "A DM9-containing protein from oyster Crassostrea gigas (CgDM9CP-3) mediating immune recognition and encapsulation." Developmental & Comparative Immunology 116 (March 2021): 103937. http://dx.doi.org/10.1016/j.dci.2020.103937.

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39

Terahara, Kazutaka, Keisuke G. Takahashi, Akifumi Nakamura, et al. "Differences in integrin-dependent phagocytosis among three hemocyte subpopulations of the Pacific oyster “Crassostrea gigas”." Developmental & Comparative Immunology 30, no. 8 (2006): 667–83. http://dx.doi.org/10.1016/j.dci.2005.09.009.

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40

Bachere, Evelyne, Yannick Gueguen, Marcelo Gonzalez, Julien de Lorgeril, Julien Garnier, and Bernard Romestand. "Insights into the anti-microbial defense of marine invertebrates: the penaeid shrimps and the oyster Crassostrea gigas." Immunological Reviews 198, no. 1 (2004): 149–68. http://dx.doi.org/10.1111/j.0105-2896.2004.00115.x.

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41

Li, Jiaxin, Weilin Wang, Qi Zhao, et al. "A haemocyte-expressed Methyltransf_FA domain containing protein (MFCP) exhibiting microbe binding activity in oyster Crassostrea gigas." Developmental & Comparative Immunology 122 (September 2021): 104137. http://dx.doi.org/10.1016/j.dci.2021.104137.

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42

Zhang, Dan, Miren Dong, Xiaorui Song, et al. "ROS function as an inducer of autophagy to promote granulocyte proliferation in Pacific oyster Crassostrea gigas." Developmental & Comparative Immunology 135 (October 2022): 104479. http://dx.doi.org/10.1016/j.dci.2022.104479.

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43

Sun, Wending, Xiaorui Song, Miren Dong, et al. "DNA binding protein CgIkaros-like regulates the proliferation of agranulocytes and granulocytes in oyster (Crassostrea gigas)." Developmental & Comparative Immunology 124 (November 2021): 104201. http://dx.doi.org/10.1016/j.dci.2021.104201.

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44

Lacoste, A., S. K. Malham, A. Cueff та S. A. Poulet. "Noradrenaline modulates hemocyte reactive oxygen species production via β-adrenergic receptors in the oyster Crassostrea gigas". Developmental & Comparative Immunology 25, № 4 (2001): 285–89. http://dx.doi.org/10.1016/s0145-305x(00)00067-7.

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45

Liu, Dongyang, Qilin Yi, Yichen Wu, et al. "A hypervariable immunoglobulin superfamily member from Crassostrea gigas functions as pattern recognition receptor with opsonic activity." Developmental & Comparative Immunology 86 (September 2018): 96–108. http://dx.doi.org/10.1016/j.dci.2018.05.007.

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46

Chen, Honglin, Xiaohui Cai, Jing Fang, et al. "TLR mediated innate immunity was essential in defense against Vibrio alginolyticus for Crassostrea gigas digestive gland." Developmental & Comparative Immunology 148 (November 2023): 105027. http://dx.doi.org/10.1016/j.dci.2023.105027.

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47

Xin, Lusheng, Chang Liu, Huan Zhang, Limei Qiu, Lingling Wang, and Linsheng Song. "The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas." Developmental & Comparative Immunology 123 (October 2021): 104145. http://dx.doi.org/10.1016/j.dci.2021.104145.

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48

Wang, Jihan, Xue Qiao, Zhaoqun Liu, et al. "A tripartite motif protein (CgTRIM1) involved in CgIFNLP mediated antiviral immunity in the Pacific oyster Crassostrea gigas." Developmental & Comparative Immunology 123 (October 2021): 104146. http://dx.doi.org/10.1016/j.dci.2021.104146.

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49

Chen, Xiaowei, Zhaoqun Liu, Yifan Gu, et al. "A hexokinase from the oyster Crassostrea gigas is involved in immune recognition as a pattern recognition receptor." Developmental & Comparative Immunology 122 (September 2021): 104083. http://dx.doi.org/10.1016/j.dci.2021.104083.

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50

Cao, Wanqing, Weilin Wang, Siqi Fan, et al. "The receptor CgIL-17R1 expressed in granulocytes mediates the CgIL-17 induced haemocytes proliferation in Crassostrea gigas." Developmental & Comparative Immunology 131 (June 2022): 104376. http://dx.doi.org/10.1016/j.dci.2022.104376.

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