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Tesis sobre el tema "Cucumoviruses"
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1
Sackey, Sammy Tawiah. "Interactions of two cucumoviruses." Title page, table of contents and summary only, 1990. http://hdl.handle.net/2440/19167.
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Ligat, Julio S. "Pathology and distribution in the host of pea seed-borne mosaic virus." Title page, contents and summary only, 1993. http://web4.library.adelaide.edu.au/theses/09PH/09phl723.pdf.
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Includes bibliographical references (leaves 82-92). Five isolates of pea seed-borne mosaic virus were compared by host range and symptomatology on 16 pisum sativum cultivars lines, 21 lines of Lathyrus and Lens spp. and several indicator species
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Hajimorad, Mohammad Reza. "Variation in alfalfa mosaic virus with special reference to its immunochemical properties." Title page, contents and summary only, 1990. http://web4.library.adelaide.edu.au/theses/09PH/09phh154.pdf.
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Includes Appendix listing other publications by the author. Includes bibliographical references (leaves 134-181). Alfalfa mosaic virus was isolated from lucerne (Medicago sativa) plants with a variety of disease symptoms. Experiments showed that each isolate was biologically distinct and that the host range and symptomatology of each isolate was affected by the environmental condition.
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Wahyuni, Wiwiek Sri. "Variation among cucumber mosaic virus (CMV) isolates and their interaction with plants." Title page, contents and summary only, 1992. http://web4.library.adelaide.edu.au/theses/09PH/09phw137.pdf.
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Includes appendix containing journal publications co-authored by the author. Includes bibliographical references (leaves 130-151). Eighteen strains of Cucumber mosaic virus, including forteen from Australia, two from the USA, and two from Japan were used in this study.
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Williams, Rhys Harold Verdon George. "Further studies on the structure and function of the cucumber mosaic virus genome : a thesis submitted to the University of Adelaide, South Australia for the degree of Doctor of Philosophy." 1988, 1988. http://web4.library.adelaide.edu.au/theses/09PH/09phw7261.pdf.
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Shi, Bu-Jun. "Expression and function of cucumoviral genomes." Title page, contents and summary only, 1997. http://web4.library.adelaide.edu.au/theses/09PH/09phs5546.pdf.
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Bibliography: leaves 104-130. The aim of this thesis is to characterise subgenomic RNAs of cucumoviruses and the functions of their encoding genes. Strains of cucumber mosaic virus (CMV) are classified into two major subgroups (I and II) on the basis of nucleotide sequence homology. The V strain of tomato aspermy virus (V-TAV) and a subgroup I CMV strain (WAII) are chosen to determine whether the 2b genes encoded by these viruses are expressed 'in vivo'. For further investigation of the 2b gene function, cDNA clones of three genomic RNAs of V-TAV are constructed. Using the infectious cDNA clones of V-TAV, a mutant virus containing only one of the two repeats is constructed.
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Geering, Andrew D. W. "The epidemiology of cucumber mosaic virus in narrow-leafed lupins (Lupinus angustifolius) in South Australia." Title page, table of contents and summary only, 1992. http://web4.library.adelaide.edu.au/theses/09PH/09phg298.pdf.
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Wispelaere, Mélissanne de. "Etude de la recombinaison chez les Cucumovirus." Paris 11, 2004. http://www.theses.fr/2004PA112270.
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La recombinaison entre génomes viraux est un processus qui participe à la conservation et l’évolution du génome viral. L’objet du travail présenté dans cette thèse était de détecter l’apparition de molécules recombinantes entre deux cucumovirus, le virus de la mosaïque du concombre (CMV) et le virus de l’aspermie de la tomate (TAV). Au cours d’une coinfection sur des plants de tabac, nous avons pu identifier par RT-PCR des molécules recombinantes dans la région 3’ non codante de l’ARN 3 de ces virus. L’observation des différents sites de recombinaison nous a permis d’identifier deux points chauds de recombinaison. L’un d’eux était situé au niveau du promoteur de synthèse du brin négatif, dans une région qui a souvent été impliquée dans la recombinaison. Le second était situé dans une région impliquée dans la génération de l’ARN 5 subgénomique. Il a été proposé que l’ARN 5 soit généré par une reconnaissance d’un promoteur subgénomique putatif. Il était ensuite intéressant de savoir si l’apparition de molécules recombinantes pouvait avoir un impact sur le développement de l’infection virale. Cette question a été adressée par l’étude des propriétés biologiques de virus possédant un ARN 3 recombiné. Lorsque les virus recombinants ont été inoculés seuls sur les deux plantes hôtes utilisées, le tabac et l’arabette, aucun symptome particulier n’a été développé. Il était intéressant de constater que ces virus avaient été générés de façon détectable au cours d’une coinfection, et qu’ils étaient capables d’infecter de façon efficace les hôtes testés. Leur impact au niveau de la population virale pourrait acquérir une importance dans le cas d’infection sur d’autres plantes hôtes<br>Recombination between viruses contributes to genome conservation and evolution. The goal of this thesis subject was to detect RNA 3 recombinant molecules between two cucumoviruses, the cucumber mosaic virus (CMV) and the tomato aspermy virus (TAV). Recombinant molecules have been detected by RT-PCR in tobacco plants coinfected with these two viruses. The recombination sites were localised within the 3’ non coding region of RNA 3. We identified two hot spots for recombination. One of them was located in the tRNA like structure, that is part of the promoter for minus strand synthesis. The other hot spot was located in the region leading to RNA 5 production. This region has been proposed to act as a promoter for RNA 5 synthesis. We then wondered if the recombinant molecules could have an influence on the viral infection. To adress this question, we studied the biological properties of viruses possessing a recombinant RNA 3. When we inoculated tobacco and Arabidopsis thaliana with these viruses, we saw no major differences in the development of symptoms. This suggests that these viruses have been generated in an efficient manner during coinfection and they were able to infect plants. Their impact on viral population could be important in other environemental conditions or during infection of other host plants
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Chen, Baoshan. "Encapsidation of nucleic acids by cucumovirus coat proteins /." Title page, contents and summary only, 1991. http://web4.library.adelaide.edu.au/theses/09PH/09phc5183.pdf.
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PORTA, CLAUDINE. "Utilisation d'anticorps monoclonaux pour l'etude des cucumovirus, des tobamovirus et des comovirus." Université Louis Pasteur (Strasbourg) (1971-2008), 1989. http://www.theses.fr/1989STR13157.
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Production d'anticorps monoclonaux diriges contre des virus de plantes pour le diagnostic d'infections virales et pour l'etude des determinants antigeniques des capsides virales. Mise au point d'un test de detection pour le virus de la mosaique du concombre, le virus des taches annulaires de l'odontoglossum et le virus de la mosaique de la tomate. Cartographie de epitopes du virus de la mosaique du tabac
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Sackey, Sammy Tawiah. "Interactions of two cucumoviruses." Thesis, 1990. http://hdl.handle.net/2440/19167.
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Hajimorad, Mohammad Reza. "Variation in alfalfa mosaic virus with special reference to its immunochemical properties / Mohammad Reza Hajimorad." Thesis, 1990. http://hdl.handle.net/2440/19057.
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Includes Appendix listing other publications by the author.<br>Includes bibliographical references (leaves 134-181).<br>vi, 182 leaves : ill., photos ; 30 cm.<br>Alfalfa mosaic virus was isolated from lucerne (Medicago sativa) plants with a variety of disease symptoms. Experiments showed that each isolate was biologically distinct and that the host range and symptomatology of each isolate was affected by the environmental condition.<br>Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Pathology, 1990
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Geering, Andrew D. W. "The epidemiology of cucumber mosaic virus in narrow-leafed lupins (Lupinus angustifolius) in South Australia / Andrew D.W. Geering." Thesis, 1992. http://hdl.handle.net/2440/21628.
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Includes bibliographical references (leaves 147-171).<br>xx, 171 leaves : ill. (some col.), photos ; 30 cm.<br>Studies factors affecting the rate of epidemic progress of cucumber mosaic virus in Lupinus angustifolius.<br>Thesis (Ph.D.)--Dept. of Crop Protection, University of Adelaide,1992
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Liu, Yu-Chin, and 劉于菁. "Molecular and biological characterization of potyvirus and cucumovirus isolated from Gloriosa superba." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/53471386533555197552.
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碩士<br>國立高雄師範大學<br>生物科技系<br>95<br>Plants of Gloriosa superba L. with yellow mosaic symptom which collected from Taichung and Pingtung showed positive reaction in indirect ELISA test with Lily mottle virus antibody. However, the diseased G. superba collected in Pingtung also reacted with Cucumber mosaic virus antibody in indirect ELISA indicatory the plants collected in Pingtung were mix infected with viruses. Under electron microscope in negatively stained of the virus of glory lily in Taichung, and the particles that were 760-860 nm in length and 10-15 nm in width. Under electron microscope purified virus with the single lesion of Chenopodium quinoa L. were infected by glory lily in Pingtung were only observed icosaheral particles with 29-31 nm diameter and the virus purification inoculated C. quinoa had a maximum and minimum absorption at 258 nm and 221 nm, respectively. Amax/Amin and A280/A260 ratios were 4.71 and 0.591, respectively. There were three bands, 4.0, 3.8 and 2.5 kb observed in the electrophoresis of double strand RNA of virus. The molecular weight of coat protein of the virus was estimated 27.5 kDa in the SDS-PAGE. Besides, with cDNA synthesis and PCR amplification, the gene of coat protein was cloned and sequenced. 657 nucleotides encoded a 24.1 kDa protein of 218 amino acid. When inquired the nucleotide and amino acid sequences of CP gene with NCBI database, it showed a high identity of 92～97% and 97～100% , respectively with other strains of Cucumber mosaic virus. Cucumber mosaic virus isolated from Gloriosa superba was first report in Taiwan. Furthermore, the virus purified from diseased leaves of G. superba in Taichung with a white opaque circular band in the centrifuge tube. The purified virus had a maximum and minimum absorption at 260 nm and 245 nm, respectively. Amax/Amin and A280/A260 ratios were 1.1 and 0.83, respectively. Electrophoretic analysis revealed that a 10 kb size of viral RNA of virus purified from G. superba. The molecular weight of coat protein of the virus was estimated 28.3 kDa in the SDS-PAGE. Besides, with cDNA synthesis and PCR amplification, the gene of coat protein was cloned and sequenced, which was found to contain 798 nucleotides encoded a 29.8 kDa protein of 266 amino acid residues. In indirect ELISA test, the LMoV antiserum reacted with the leaves of virus-infected G. superba. When inquired the nucleotide and amino acid sequences of CP gene with NCBI database, it showed the nucleotide and amino acid identities of 61% and 58% with LMoV (NC_005288), respectively. The result indicated that the virus of G. superba in Taichung and LMoV were different viruses; and throught RT-PCR which were amplified from total RNA of diseased leaves with primers which designed by CP sequences of the virus and LMoV, respectively. The result also indicated this virus and LMoV were different viruses,and the virus may only have serological relationship with the LMoV. Since the evidence showed that this virus was a new virus on glory lily named as Glory lily mosaic virus（GLMV）temporaily.
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Hung, Yi-Fang, and 洪怡芳. "Mutational studies on the replication of satellite RNAs associated with cucumber mosaic cucumovirus." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/78795951894544150965.
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碩士<br>國立中興大學<br>農業生物科技學研究所<br>90<br>Abstract
Cucumber mosaic virus (CMV), the type member of the Cucumovirus, is a plant virus with a tripartite genome. Some CMV strains contain a small RNA that has been demonstrated to be a satellite RNA (satRNA). SatRNAs are dependent on helper virus for replication, encapsidation, and transmission, but share little or no sequence similarity to the helper virus. The specific aim of this study is to elucidate the minimum required sequence signals on satRNAs for high efficiency replication to gain further insight into the biological functions of satRNAs. Deletion mutations of satRNA were created around the BamHI and NcoI sites. The transcripts of mutants were synthesized in an in vitro transcription system and were used to inoculate Nicotiana tabacum and Nicotiana benthamiana with the helper virus, CMV-NT9, which is free of satRNAs. The viability of mutants was analyzed by double-stranded RNA analyses. The nucleotide sequences of the mutant satRNAs progenies were investigated using RT-PCR followed by DNA sequencing. The first-generation progenies that contain mutations near the BamHI site could maintain 3 to 20 nucleotides deletions. The mutants with 31 nucleotides deletions near the NcoI site maintained the replication ability. It is found that 30%~70% of the first-generation progenies have reversed to wild type, while the others still maintain deletion sites between nucleotide positions 47 to 55. For the second and third-generation progenies, 30%~100% have reversed to wild type and 3 to 4 different nucleotide insertions were found between nucleotide positions 45 to 47, indicating that the nucleotides between positions 47 to 57 are not required for efficient replication with in N. tabacum. However, from N. benthamiana inoculation test, the first-generation progenies maintained similar deletion sites of the inoculum, and it is found that 55%~100% of progenies contained exactly the same mutation sites. The longest deletions allowed were 87 nucleotides around BamHI site and 31 nucleotides around NcoI site. These results suggested that the host plants played an important role in the evolution and selection of satRNA quasispecies. By mutation and recombination, satRNA may adapt to different hosts. These results might be applied in the understanding of helper virus replication and development of satRNA-based transient expression vector systems.
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Le, Roux Karin 1974. "Characterisation and detection of viruses (Cucumovirus, Potyvirus) infecting vanilla in Réunion Island and Polynesian Islands." 2005. http://hdl.handle.net/2292/1289.
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Natural vanilla (Vanilla planifolia, V. tahitensis) is economically important for a number of producing countries, but viral diseases are prejudicial to its successful cultivation. Techniques are required to detect viruses in vanilla plants in order to establish quarantine procedures and sources of virus-free planting material. This study contributed to the progress in managing viral diseases of vanilla by firstly identifying Cucumber mosaic virus (CMV, cucumovirus) as causing severe distortion, stunting, sterility and sometimes death of vanilla plants. Vanilla CMV isolates from French Polynesia (Pacific Ocean) and Réunion Island (Indian Ocean) were classified in CMV subgroup IB, adding to the short list of subgroup IB isolates detected outside of Asia. Two isolates were putatively classified in subgroup IA, suggesting that CMV in vanilla is still evolving and/or that vanilla is infected from different sources. Subgroup II CMV from New Zealand was also experimentally infectious to V. planifolia, showing that vanilla crops should be protected from all potential sources of CMV inoculum. Existing serological and molecular detection rests were performant for the detection of CMV directly from vanilla tissue. Secondly, this study provided the first coat protein sequence information for Vanilla mosaic virus (VanMV, Potyvirus). A Cook Islands isolate (VanMV-CI) and a French Polynesia isolate (VanMV-FP) had distinctly different coat proteins. The VanMV-FP CP N-terminus contained a stretch of amino-acid repeats (GTN) typical of natively unfolded proteins. This GTN stretch was located downstream of a DVG motif (which replaced the more common aphid transmission DAG motif), suggesting a role in improving aphid transmission, or regulating formation of the HC-virus complex. CP core nucleotide sequence identities indicated VanMV-CI and VanMV-FP were strains of Dasheen mosaic virus (DsMV). In contrast, CP amino-acid sequence homologies between VanMV- CI and DsMV were intermediate between strains and species, and CP amino-acid homologies between VanMV-FP and DsMV were typical of distinct species. In addition, VanMv-CI and VanMv-FP had characteristic 3'NTR sequences and NIb/CP cleavage sites, and only infected vanilla. Hence, it is proposed that VanMV-CI and VanMV-FP are considered new Potyvirus species and named Vanilla mosaic Cook Islands virus and Vanilla mosaic French Polynesia virus. Alternatively, the two isolates may be grouped under the name Dasheen mosaic virus-Vanilla (DsMV-V) and distinguished from Dasheen mosaic virus-Dasheen(DsMV-D). Primers to VanMV-CI and VanMV-FP were designed and permitted RT-PCR detection of the viruses directly from vanilla tissue. VanMV-Cl and VanMV-FP could be differentiated from DsMV and Watermelon mosaic virus (WMV-Tonga), and differentiated from each other by comparison of amplicon size. Long-term specific potyvirus diagnosis is however expected to be difficult due to potyviral variability in vanilla. Future research should concentrate on techniques such as microarrays to permit simultaneous detection combined with specific identification of potyvirus species. Such techniques would be beneficial to viral disease management in vanilla and many other crops.
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Mao, Yie-Chie, and 毛乙智. "The Study on the Interaction between Coat Proteins and Satellite RNA of Cucumber Mosaic Cucumovirus." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/55327153997726051916.
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碩士<br>國立中興大學<br>農業生物科技學研究所<br>89<br>The interactions between satellite RNAs (satRNAs) and coat proteins of the helper virus, cucumber mosaic cucumovirus (CMV), were studied to elucidate the corresponding regions in coat proteins and satRNAs responsible for recognition. This work is of vital importance for the successful development of satRNA-based transient expression system. To facilitate efficient detection and analysis of interactions between nucleic acids and protein molecules, a system based on enzyme linked immunosorbent assay (nucleoprotein binding-ELISA, NB-ELISA) was developed and applied to the fast and efficient analysis of interactions between satRNAs and coat proteins of CMV. The satRNA from CMV-C strain (C-sat) and coat proteins of CMV-NT9 strain were used to optimize and standardize the NB-ELISA procedures. A positive, linear correlation was observed between the concentrations of coat proteins of CMV and the observed OD405 value. The C-sat showed binding preference for CMV coat proteins over non-related proteins, such as tobacco mosaic virus coat proteins, bovine serum albumin, and healthy plant extracts. Sucrose density gradient centrifugation assay indicated that the satRNA might attach to the outer surface of the virus particles, besides being encapsidated inside. The result of time course study suggested the binding of satRNAs to coat proteins proceeded in a cooperative manner as a function of time. The coat protein genes of CMV-NT9, CMV-Gem and CMV-M48 strains were cloned and sequenced for further investigation of interactions with satRNAs. The N-, C-terminal and internal fragments (from amino acid number 1 to 76, 176 to 218, and 77 to 169, respectively) of CMV-NT9 coat proteins were subcloned into plasmid pETblue2 for efficient expression in Escherichia coli to study the regions on the coat proteins responsible for the interaction with satRNAs. The N-terminal fragment of coat protein was shown to be the sole required region to recognize satRNAs. Terminal and serial internal deletion mutants of C-satRNA were used to investigate the nucleotide sequences that are required for the proper recognition by the CMV coat proteins. The result indicated that CMV coat proteins interacted with both DNA and RNAs, single- or double-stranded, without evident specificity. These results provided further insight into the biological functions of satRNAs to facilitate the better design of efficient gene-transfer vectors and disease management strategies.
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Chun-Nan, Chou. "Biological, molecular and symptom determinater analysis of Cucumber mosaic cucumovirus isolates associated with banana mosaic disease." 2007. http://www.cetd.com.tw/ec/thesisdetail.aspx?etdun=U0001-2401200710424600.
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Chou, Chun-Nan, and 周俊男. "Biological, molecular and symptom determinater analysis of Cucumber mosaic cucumovirus isolates associated with banana mosaic disease." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/99481844049366127120.
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碩士<br>國立臺灣大學<br>植物病理與微生物學研究所<br>95<br>Abstract
Banana samples with mosaic symptoms were collected from southern Taiwan, and 12 type isolates of Cucumber mosaic cucumovirus associated with banana mosaic disease were established. The 12 CMV isolates were characterized into three types, (I-III), by the symptoms induced by CMV on bananas. The type I-infected bananas initially showed chlorosis with little necrosis streak, and later the full expanded leaves showed severe green mosaic with distortion either becoming slender leaf. Type II induced yellow mosaic with severe necrosis on leaves. The severe necrosis on pseudostem will eventually cause heart rot in field. Type III induced leaf chlorosis, however, the full expanded leaves show severe green mosaic without leaf deformation. For biological characterization of the collected CMV, we followed the methods described by Wahyuni (1992) and Wu (1994) using cowpea and N. glutinosa as differential hosts. When Nicotiana. glutinosa was used as differential hosts, Type I CMV induce severe green mosaic and parkling, or fern leaf-like leaf distortion. Type II CMV induce chlorosis on newly emerging leaves, and later the newly expanded leaves were deformed and caused necrosis spot on shoot tip and leaves. Type III only induce mild mottling and did not induce leaf distortion. When cowpea was used as the differential hosts, most Type I and Type II isolates can not systematically infect cowpea (belonging to as Solanaceae common strains). One isolate of type I (sm1) and the only isolate of type III (PL) can systematically infect the cowpea showing mosaic, (belonging to Legume strain). The phylogenetic analysis using CMV-CP sequences revealed that Legume and Solanaceae common strains were distinct groups. In addition to previously identified subgroups CMV IA, IB and II, new subgroups IS, IC and IT were confined. Members within these groups were geographic related, which suggest founder effect play roles in CMV evolution. One satellite RNA associated with isolate 22 without causing attenuate symptom on banana and N, glutinosa was identified. During characterization of CMV by differential host, isolates 20 caused stunting by repressing the internodes growth of N. benthamiana. By pseudorecombination between CMV 20 and 25 isolates the symptom determinater was found locating on RNA 2 of CMV 20. Following express the CMV proteins 2a and 2b protein individually or in combination, we found that the symptom determinater was controlled synergistically by both 2a and 2b encoded by RNA2 of 20.